Gastric Surface Cells Research Articles

Studies on the Effects of Histamine upon the Secretory Function of the Gastric Gland Cells

The author injected subcutaneously the histamine liberators-Sinomenine-HCl, Trypsin, Dextran - in an attempt to study the effects of histamine released in vivo on the secretory function of the gastric gland cells.In the gastric peptic cells, the production and discharge of secretion granules similar to those of histamine injection were observed after the injections of histamine liberators. But it is noticed that the productin vacuoles in the gastric surface cells, which have never decreased by the injection of histamine, decrease positively after the injections of histamine liberators and that the secretory function of the peptic cells seems to be effected by productin discharged besides by histamine released. Here the gastric hormone productin (FUJIE) which was contained in the gastric surface cells is considered to be the substance which is discharged by the histamine liberators, and it remains to demonstrate the similarity between productin and histamine.In the gastric parietal cells, the active secretory function can be observed after the injections of histamine liberators, but compared with those of the peptic cells, they have no characteristics.Histamine values in the blood are highest in case of Sinomenine-HCl and are lowest in case of Dextran. Contrasting the low value of histamine with the secretory function of the gastric peptic cells, a strong anti-histamic action in vivo can be presumed.

ヒスチヂンが胃腺細胞分泌機能に及ぼす影響に関する実験的研究

Histidine hydrochloride, 1mg and 5mg, was added to non-protein diet so that the effect of histidine on the secretory function of the gastric gland cells might be ascertained, and the results obtained have been compared with each together in cases of 1. giving histidine diet, 2. non-protein diet and 3. normal artificial diet for 10 and 20 days. In case of giving histidine diet, though the absolute quantity of protein was lacking as in case of giving non-protein diet, the fluctuation of the productin vacuoles after the administration of the diet was similar to that in the normal case. The secretory function of the gastric peptic and parietal cells showed ont only an almost similar activity to that of the normal case but sometimes an activity superior to that of the latter case, and these activities considerably differed from activities discerned in case where a non-protein diet was given.In order to ascertain whether the gastric gland cells have a faculty to perform the secretory function or not, after giving non-protein diet, histamine hydrochloride was injected first into the still unfed rats after they had been fed with non-protein diet for 10 and 20 days. The results obtained here demonstrated that the secretory function with the exception of the discharge of secretory substance from the peptic cells, were as sound or almost as sound as normal. And the mixture of acetylcholine and eserine was, secondarily, injected into the same conditioned animals so that the gastric hormone productin might be artificially discharged into the blood. The results obtained in this case have demonstrated only a little discharge of productin vacuoles from the gastric surface cells and a little transformation of plastosomes in the peptic cells, which can hardly be considered the functional movement in the production of secretion granules.According to these results, it may be concluded that the lack of protein in diet brings about the functional inhibition of the discharge of the secretory substance from the gastric peptic cells and also of the discharge of productin into blood from the gastric surface cells and that histidine in the diet can supplement these inhibitions.

Histochemical Research on the Content in the ‘Productin’ Vacuoles (FUJIE) of the Gastric Surface Cells. II

Histochemical Research on the Content in the ‘Productin’ Vacuoles (FUJIE) of the Gastric Surface Cells. II

Histochemical Research on the Content in the‘Productin’Vacuoles (FUJIE) of the Gastric Surface Cells. I

Histochemical Research on the Content in the‘Productin’Vacuoles (FUJIE) of the Gastric Surface Cells. I

Studies on the Dietetic Characteristics of the Secrsetory Functional Images in the Pancreatic Cells

Rats were fed with experimental artificial diet which contained constant quantity of fat, different quantities of protein and carbohydrate as represented in table 1, then the secretory function of the pancreatic cells were observed morphologically.In the feeding with non-protein diet (0% protein and more than 80% carbohydrate), the cell function was extremely low, and it was demonstrated that the secretory function of the pancreatic cells for carbohydrate-digestion might be slight. In the prolonged feeding many degenerated cells appeared, which might be owing to a lack of protein, not to a functional degeneration. In the feeding with high protein diet (80% protein and less than 10% carbohydrate) the cell function was extremely vigorous and continued for a long time after the administration. In the prolonged feeding the degeneration could be seen in many cells, which might be owing to the functional overwork. In the other feeding with 20%, 40% or 60% protein diet, the secretory function of the pancreatic cells became more vigorous and more continuous according to an increment of the protein content. These characteristics of the cell function, especially the dietetic characteristics in the production of the zymogen granules in the cells are explainable with the superior or inferior secretion of productin at each time, which can be presumed from the fluctuation of productin vacuoles in the gastric surface cells. It was concluded that the dietetic characteristics in the production of zymogen granules must be attendant to the characteristic secretion of productin due to the diet content.The discharge of zymogen granules was almost concomitant with the production-performance. Therefore it was also characteristic in each case. The author compared this with the results of other investigators and reached the conclusion that the characteristic secretion owing to the diet content could be also applicable to the secretin secretion (BAYLISS and STARLING) as well as to the productin secretion.

Stimulation of cell-free gastric mucus by the topical application of acetylcholine.

SummaryThe topical application of acetylcholine or related parasympathomimetic drugs to the mucosa of canine fundic pouches results in the secretion of alkaline opalescent viscid mucus which is free of columnar cells. These studies confirm our previous conclusion, that the exfoliation of gastric surface cells is not an essential part of the process of mucus secretion.