This study investigated the antibiotic resistance profile of the L. garvieae isolated strains from Jordanian dairy products. Disk diffusion method was used. In particular, different antibiotics were tested against L. garvieae; the antibiotic disks were Trimethoprim (5 µg), Clindamycin (2 µg), Nitrofurantoin (300 µg), Erythromycin (15 µg), Ampicillin (15 µg), Trimethoprim/Sulfamethoxazole (1.25/23.75 µg Polymyxin B (300U) and Tetracycline (30 µg). The results indicated high and statistically significant effects of Trimethoprim, Nitrofurantoin and Trimethoprim/Sulfamthoxazole on L. garvieae. Trimethoprim had the greatest antimicrobial effect on L. garvieae strains. All of the strains were sensitive to this antibiotic. Trimethoprim showed 20 mm inhibition zone in some strains. On the other hand, there were no antibiotic effects of Clindamycin and Polymyxin B on L. garvieae. Slight and statistically insignificant effects were found of other tested antibiotics. The study recommended using Trimethoprim antibiotic against L. garvieae because it inhibited its growths in all isolated cultures.