Garlic Research Articles

In vitro and In silico Network Pharmacology Analysis of Eugenol from Syzygium aromaticum Against Escherichia coli

ABSTRACT Background: In this era, Escherichia coli is an ambivalent microbe and commonly causes fecal contamination. Eugenol from clove oil has also demonstrates antibacterial effects that may aid in combating against the microorganisms that cause tooth infections. Aim and Objectives: The current study provides a comprehensive analysis of the pharmacological characteristics of eugenol, a significant bioactive substance extracted from clove (Syzygium aromaticum). The present study aimed to target the genes from E. coli identified through network pharmacology analysis of eugenol and antibiofilm effect of eugenol from clove oil against E. coli. Materials and Methods: In the present study, eugenol was isolated from clove identified through Fourier transform infrared spectroscopy analysis. The antibacterial and antibiofilm activity of eugenol was performed against E. coli were investigated. Targets were identified for Eugenol from clove oil through string database and mapped with E. coli pathway. Molecular docking analysis was carried out for the identified targets and eugenol. Results: The best antibacterial activity of eugenol from clove oil was found against E. coli and evaluated by zone of inhibition and minimum inhibitory concentration analysis at 10 μL. The antibiofilm assay against resistant E. coli was determined by concentration of 10 μL and inhibited in 100% dilution. The top five active targets from E. coli was identified by network pharmacology and docking analysis shows the good binding interaction between targets and eugenol. Conclusion: The research provided in this study lays the foundation for eugenol’s anti-bacterial property with various biofilm forming targets namely Acetyl-CoA acetyltransferase, Acetyl-CoA carboxylase-B, Acetyl-CoA carboxylase-C, Enoyl-[acyl-carrier-protein] reductase [NADH], and 3-oxoacyl-[acyl-carrier-protein] synthase 1. Additional in vivo approach validation is required in future to support the current study.

Extraction and Identification of the Main Components of Cloves (Syzygium aromaticum L.) Oil Extract and its Antimicrobial Activity against Methicillin-resistant Staphylococcus aureus strain

خلفية: تزايد استخدام الأدوية العشبية بدلاً من الأدوية التقليدية نتيجة مقاومة المضادات الحيوية ، مما يشكل خطراً على الصحة العامة. الأهداف: دراسة التركيب الكيميائي واخنبارالنشاط المضاد للبكتيريا في المختبر لمستخلص زيت القرنفل ضد جرثومة المكورات العنقودية الذهبية المقاومة للميثيسيلن MRSA)) . الطريقة: تم الحصول على مستخلص زيت القرنفل باستخدام التقطير المائي بواسطة جهاز Clevenger. بعد ذلك ، تم إجراء التحليل الكيميائي النباتي عن طريق التحليل الكروماتوجرافي-مطياف الكتلة. تم إجراء فحص في المختبر لمضادات الميكروبات لزيت القرنفل باستخدام طريقة انتشار القرص المزدوج ، وطريقة التخفيف الدقيق ، وحركة منحنى وقت القتل في المختبر. النتائج: بلغت نسبة استخلاص مستخلص القرنفل 50٪. وقد أظهر التحليل الكيميائي النباتي وجود مكونات مختلفة وبشكل رئيسي هي Caryophyllen و Humulene و Eugenol. حيث تبين ان المكورات العنقودية الذهبية كانت حساسة لمستخلص القرنفل بتركيزات مختلفة علاوة على ذلك ، وجد أن أقل تركيز مثبط (MIC) وأقل تركيز مبيد للجراثيم (MBC) كان 2.5 ميكروغرام / مل ، 5.0 ميكروغرام / مل على التوالي. الاستنتاجات: أظهرت الدراسة الحالية أن مستخلص القرنفل أظهر نشاطًا جيدًا مضادًا للبكتيريا في المختبر

Response of plant growth regulators on growth and yield traits of garlic (Allium sativum L.) Cv. Yamuna Safed

Response of plant growth regulators on growth and yield traits of garlic (Allium sativum L.) Cv. Yamuna Safed

Effect of Nitrogen in Premature Sprouting of Garlic (Allium sativum)

A field experiment was carried out at Regional Spices Research Centre, Bangladesh Agricultural Research Institute, Magura during 2021-22 and 2022-23 to find out the effect of nitrogen in premature sprouting of garlic. The two factor experiment was designed in Randomized Complete Block Design (RCB) with three replications. The treatments comprised of three varieties of garlic (BARI Rashun-1, BARI Rashun- 3 and Advance line AS Mag-001) and four dose of nitrogen. Significant differences regarding yield and yield attributes were observed among different treatments. The highest plant height (92.00 cm in 2021-22 and 94.08 in 2022-23), individual bulb weight (30.33 g in 2021-22 and 31.58 g in 2022-23) and yield per hectare (13.30 t/ha in 2021-22 and 13.48 in 2022-23) were obtained from the treatment T4V3 (AS Mag-001 line with nitrogen@ 235kg/ha). The lowest plant height (69.67 cm), number of leaves per plant (7.67), individual bulb weight (23.67 g) and yield per hectare (8.31 t/ha) from the treatment T2V1 (BARI Rashun-3 with nitrogen @ 185kg/ha). The highest number of sprouted plant (43) with maximum incidence (22.88%) were observed in T4V3 (AS Mag-001 line with nitrogen @ 235kg/ha)) where the lowest number of sprouted plant (7) and with minimum incidence (4.55%) were observed in T1V2 (BARI Rashun-3 with nitrogen @ 160kg/ha).

Characterization of pectolytic bacteria causing soft rot on sugar beet in the west of Iran

AbstractBacterial soft rots are amongst the most economically important diseases that cause significant yield losses in various crops. A clear identification of the causal agents is required to develop management strategies. In 2022 and 2023, after the widespread occurrence of soft rot disease in sugar beet fields in the Firuzan region, Hamedan Province, Iran, the current study was conducted to identify and characterize the causal agents. A total of 38 bacterial isolates were obtained from sugar beet plants with soft rot symptoms and subjected to biochemical and phenotypical characterization and pathogenicity tests on healthy slices from potato tubers and sugar beet taproots. Twelve isolates from this study along with the type strain Pectobacterium carotovorum ICMP 5702 were selected for subsequent screening by BOX‐PCR. Six isolates were chosen for sequencing the 16SrRNA gene and phylogenetic analysis. In BLAST analyses, the pathogenic isolates S2, S9, S22, S11, S23 and 111 showed the highest similarity values (≥99%) with the reference sequences of Raoultella terrigena, Klebsiella michiganensis, Erwinia (Winslowiella) iniecta, Pantoea agglomerans and P. carotovorum, respectively. These isolates induced soft rot and maceration on plant materials from five species (bell pepper fruits, garlic cloves and slices from sugar beet taproot, potato tubers and carrot roots) 72 h after inoculation. In the greenhouse experiment, they caused soft rot symptoms in the taproots of 2‐month‐old sugar beet plants 3 weeks after inoculation. These soft rot agents had Gram‐negative, catalase‐positive and oxidase‐negative reactions, grew under both aerobic and anaerobic conditions, tolerated and grew at 37°C and 5% NaCl. In general, the results of this research showed that in addition to Pectobacterium, four other genera from the order Enterobacterales including Erwinia, Pantoea, Klebsiella and Raoultella, are amongst the causes of soft rot in sugar beet in Firuzan region of Hamedan.

Synthesis of Silver Nanoparticles Using Azadirachta indica and Syzygium aromaticum Extract and Its Antibacterial Action Against Enterococcus faecalis: An In Vitro Study.

Introduction Nanotechnology is the study of manipulating matter at the atomic scale involving particles smaller than 100 nm. Silver nanoparticles (AgNPs) are gaining popularity across diverse sectors including medical, food, healthcare, consumer goods, and industrial fields due to their distinctive physical and chemical characteristics. The eco-friendly synthesis of AgNPs offers a straightforward, cost-effective, and environmentally benign method devoid of hazardous chemicals. Methodology Eighty milliliters (mL) of silver nitrate mixed with 20 mL of Azadirachta indica and Syzygium aromaticum plant extract underwent two days of magnetic stirring for AgNP synthesis. Characterization was done via ultraviolet-visible (UV-vis)-spectroscopy (300-700 nm), and antimicrobial properties, which were checked with Enterococcus faecalis, were assessed using the agar-well diffusion method. Results The change in color and peak observed in the UV-vis spectrum confirmed the successful synthesis of AgNPs. Both neem and clove extract-mediated synthesis of AgNPs exhibited antibacterial activity against E. faecalis. However, neem extract synthesized AgNPs displayed a larger inhibitory zone diameter and lower minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values compared to those synthesized using clove extract. Conclusion Incorporating neem and clove extracts in AgNP synthesis offers a practical, eco-friendly, and cost-efficient method with notable efficacy. These AgNPs exhibit antibacterial activity against E. faecalis, suggesting their viability as potent antibacterial agents for addressing oral pathogens. Their sustainable synthesis underscores a promising avenue for developing effective antimicrobial solutions in oral healthcare.

Antioxidant and neuroregenerative roles of Allium sativum L. in promoting central nervous health

Antioxidant and neuroregenerative roles of Allium sativum L. in promoting central nervous health

Comparação das distribuições Normal, Logística, Laplace e t de Student para o erro experimental na descrição bayesiana do acúmulo de matéria seca de Allium sativum

ABSTRACT: This study assessed distributions associated with Bayesian nonlinear modeling error in the description of total plant dry matteraccumulation (TDMA) of Allium sativumas a function of days after planting (DAP). According to the DIC criterion, Logistic and Gompertzmodels that use student’s t distribution error exhibited the highest DIC with logistic error distribution. In general, the difference of DIC in all the scenarios was not more than 5.The Bayes factor (BF) criterion showed no difference in the Logistic and Gompertzmodel fit when four distributions are used for the errors, where BF values do not exceed 2. Posterior distributions and the usual estimators of Logistic and Gompertz model parameters were similar even forvaried error distribution. In summary, there was no difference in the use of 4 distributions associated with the modeling error of garlic plant growth by the Bayes factor, whereby the results showed that alternating between error distributions significantly changes the number of Markov Chain Monte Carlo (MCMC) iterations.

Efek Ekstrak Bawang Putih (Allium sativum L.) dalam Menekan Pertumbuhan Bakteri Salmonella typhi secara In vitro

Salmonella typhi is a Gram-negative bacterium that has no spores, moves with peritrichic flagellum, is facultative intracellular and facultative anaerobic. Garlic (Allium sativum) has been known for a long time to be used as a seasoning and medicine. The antibacterial activity of garlic can control pathogenic bacteria, both gram-negative and positive. To determine the effectiveness of Salmonella typhi bacteria growth given garlic extract (Allium sativum). This research is a true experiment post test using disc diffusion method. The higher the concentration of garlic extract (Allium sativum), the greater the inhibition zone obtained. The highest concentration of 100% garlic extract against Salmonella typhi bacteria has an inhibition zone of 14.4 mm with sensitive interpretation. Garlic extract (Allium sativum) has antibacterial effectiveness against the growth of Salmonella typhi bacteria

Chronic stress-induced neurodegenerative changes in Wistar rats: Hippocampal evidence and the mitigating effects of Allium Sativum

Objective: Stress triggers a biological response perceiving a threat, chemicals and hormones surge throughout the body, after long-term exposure, stress accelerates cell ageing, immune senescence, and some age-related diseases such as neurodegenerative disorders and osteoporosis. This study finds possible effects of Allium sativum ethanolic extract on the hippocampus of chronic stress-induced neurodegenerative changes in male Wistar rats. Methods: Forty Wistar rats were grouped into eight of five (n=5). Group A - control group (no stress and treatment), Group B - 100mg/kg of Allium sativum (No stress), Group C - 250mg/kg of Allium sativum (No stress), Group D - 500 mg/kg Allium sativum (No stress), Group E - Stress alone + No treatment, Group F - 100mg/kg of Allium sativum (Stressed), Group G - 250mg/kg of Allium sativum (Stressed) and Group H - 500mg/kg of Allium sativum (Stressed), all for 14 days. Animals were euthanized humanely and the hippocampus was excised and fixed in 10% formalin solution for histological processing while the part used for biochemical assay was homogenized in phosphate buffer before centrifugation. Results: Chronic exposure of rats to stress elicited reduced body and brain weights, altered neurotransmitter levels and overall neurobehavioural functions, while also resulting in marked histomorphological distortions in the hippocampus. Interestingly, supplementation with Allium sativum significantly (p<0.05) mitigated biochemical, neurobehavioural, and hippocampal aberrations. Conclusion: Notably, data obtained from this study showed that Allium sativum may be a very effective adaptogen in curtailing the deleterious neurodegenerative changes associated with chronic stress.

Supplementation species effect on the phenolic content and biological bioactivities of the decocted green tea

Background: Green tea, produced from the leaves of Camellia sinensis, is the most widely consumed beverage in the world after water, and it is widely sold and popular owing to its flavor, and its health benefits. Spices have been used by consumers worldwide to improve flavors of food including tea. Phenolics are the most common phytochemical found in herbal teas and are beneficial in promoting health or preventing diseases. Aims: This study aims to investigate the impact of the addition of mint (Mentha piperita L.), cinnamon (Cinnamomum verum), star anise (Illicium verum), ginger (Zingiber officinale), white mugwort (Artemisia herba alba) and clove (Syzygium aromaticum) on green tea biological properties, and on consumers’ acceptance. Subjects and Methods: Different green tea blends were formulated with 10% of each proposed spice. Total phenolic and flavonoid contents of formulated blends were measured by Folin-Ciocalteu and aluminum chloride methods, respectively. The effect of these herbs supplementation on antioxidant activity of green tea was determined using three different methods, namely DPPH assay, ferric reducing antioxidant power (FRAP), and total antioxidant capacity (TAC). In vitro antibacterial activity was assessed by the disc-diffusion method. Consumers’ acceptance of tea blends was evaluated by a sensory hedonic analysis performed by 127 panelists. Results: The results revealed significant variability among tea blends in terms of both total polyphenols (from 591.951 to 1027.387 mg GAE/L) and flavonoids (from 140.78 to 279.329 mg QE/L). As regards the antioxidant activity, control (no supplemented) green tea exhibited the highest ferric reducing antioxidant power (RC0.5 = 27.25 mg AAE/g DE). Interestingly tea blends supplemented with white mugwort and ginger demonstrated the highest DPPH• scavenging effect with IC50 of about 3.37 and 3.38 mg AAE/g DE, respectively, while when supplemented with star anise, it showed the highest TAC (66.143 mg AAE/g DE). In the assessment of the antibacterial effect, control green tea showed the best efficacy, except for Listeria innocua which was particularly sensitive to samples flavored with star anise and clove. The sensory evaluation revealed that clove-flavored tea was the most preferred by the panelists (31.75%), closely followed by ginger green tea (30.95%), green tea and star anise flavored green tea (25.39%). Conclusion: The obtained results indicate that adding some species to green tea significantly enhance its phenolic content, boost its antioxidant capacity and improve its sensory properties. Keywords: Flavored green tea; polyphenols; antioxidant activity; antibacterial activity; sensory characteristic.

Antifungal activity test of single clove garlic and single clove black garlic (Allium sativum L.) against Trichophyton rubrum

Antifungal activity test of single clove garlic and single clove black garlic (Allium sativum L.) against Trichophyton rubrum

Analysis of the Effect of Black Garlic (Allium sativum) Extract on Ovarian Follicular Atresia, Endometrial VEGF Expression, and Fallopian Tube Epithelial Cell Count in Rats (Rattus norvegicus) Exposed to Cigarette Smoke

Cigarette smoke exposure significantly impairs reproductive function in Rattus norvegicus. This study evaluated the protective effects of black garlic extract against such damage. Using a post-test-only control group design, 25 female Wistar rats were divided into five groups and exposed to cigarette smoke with or without varying doses of black garlic extract. After four weeks, results indicated that black garlic extract significantly increased Vascular Endothelial Growth Factor expression, enhanced fallopian tube secretory epithelial cell counts, and reduced ovarian follicular atresia in rats exposed to cigarette smoke. The group receiving 50 mg/kgBW of black garlic extract showed the most significant improvements. Statistical analysis, including One-way ANOVA, revealed significant differences between groups. Normality was assessed using the Shapiro-Wilk test, and homogeneity was confirmed with the Levene test. Significant decreases in ovarian follicular atresia (p < 0.05) and increases in Vascular Endothelial Growth Factor expression (p < 0.05) and secretory epithelial cell counts (p < 0.05) were observed in the 50 mg/kgBW treatment group compared to controls. In conclusion, black garlic extract offers dose-dependent protection against cigarette smoke-induced reproductive damage, with 50 mg/kgBW being the optimal dose. Further research should explore molecular mechanisms, long-term toxicity, and clinical applications in humans.

The Effects of Black Garlic on Ovarian Malondialdehyde, Oviduct Muscle, and Endometrial Arterioles in Smoke-Exposed Rats

The effect of cigarette smoke exposure on reproductive health has been widely studied, showing various physiological disorders induced by free radicals and oxidative stress. This study aimed to assess the protective effect of ethanol extract of black garlic (Allium sativum) on some reproductive parameters of female rats Rattus norvegicus exposed to subacute cigarette smoke. A proper experimental method with a post-test-only control group design was used, involving 25 rats divided into five groups with three different doses of the extract. The rats were exposed to cigarette smoke and then treated for 28 days. Results showed a significant decrease in ovarian Malondialdehyde levels and increased fallopian tube smooth muscle thickness in the extract-treated group. In addition, black garlic extract successfully increased the number of endometrial arterioles in the group exposed to cigarette smoke and extract, in contrast to the group exposed to cigarette smoke only. This study concludes that black garlic extract has the potential to be a protective agent against oxidative damage in the reproductive system of rats exposed to cigarette smoke, with effectiveness that depends on the dose of extract given. Further studies should investigate the long-term effects and optimal dosing of black garlic extract in humans. Additionally, exploring the molecular mechanisms behind its protective properties could enhance its application in reproductive health management.

Impact of Organic Manures and Slow-Release Nitrogen Fertilizer (Enciabein) on Productivity and Quality of Garlic (Allium Sativum, L.)

Impact of Organic Manures and Slow-Release Nitrogen Fertilizer (Enciabein) on Productivity and Quality of Garlic (Allium Sativum, L.)

Evaluation of Garlic Germplasm for Resistance to Leaf Blight Caused by Stemphylium vesicarium

Leaf blight caused by Stemphylium vesicarium is one of the most important fungal diseases of garlic (Allium sativum L.) worldwide, which results in a reduction of quality and yield. The breeding of resistant cultivars is an efficient approach to decrease the use of chemical fungicides and minimize crop losses. In this study, to find the resistant garlic resources against S. vesicarium, we evaluated the resistance degree of 20 garlic germplasms. To do this, garlic seedlings at four-leaf stage were rubbed with nonabsorbent cotton and then inoculated with spore suspension (3.0×105 spores/ml of potato dextrose broth) of S. vesicarium by spray method. Three to seven days after inoculation, the infected leaf area (%) of garlic seedling was measured. ‘Daeseo’ and ‘Namdo’ were included as susceptible and resistant control cultivars, respectively. After 3 to 7 days of incubation, the infected leaf area (%) of garlic seedling was measured. Our results showed that IT245512, IT245528, and IT244068 lines exhibited the highest resistance against S. vesicarium, whereas IT257134 and IT253043 lines were more susceptible than the susceptible cultivar ‘Daeseo’. Based on the results, the resistant genetic resources selected in this study can be used a basic material for resistant garlic breeding system against leaf blight.

An Image Identification System for Agricultural Items Based on Machine Vision

The purpose of this work was to use machine learning classification models and hyperspectral camera technologies to create a model of surface damage to garlic. 140 of the 184 garlic plants of which 44 were used for test validation were pre-treated for surface damage. First, we examined the data in ENVI under various damage scenarios using the normalised vegetation index (NDVI) approach. 579 pixels were then chosen for the training of the logistic regression model. Finally, we used 54 garlic bulbs to practically validate the model. Although tiny regions could not be precisely identified, the mouldy portion of the garlic’s surface could be identified using the NDVI technique. 90% accuracy was attained using the 90% classification model constructed using the logistic regression approach. Garlic’s surface damage, even at first mild ones, was correctly identified. The creation of this model for identifying garlic damage lowers the cost of detecting garlic damage and broadens the use of hyperspectral technologies in garlic detection.

Targeting the Coronavirus SARS‑CoV‑2’s Envelope, Nucleocapsid, and Spike/ Spike RBD Protein: Computational Insight from Multiple Bioactive Compounds as Potential Anti-Viral Drug Candidates

Global health, social, and economic systems have been seriously threatened by the coronavirus disease (COVID-19) pandemic. In addition to the increasing number of deaths, thousands of COVID-19 survivors continue to experience life-altering illness. This study aimed to evaluate multiple bioactive compounds from various indigenous medicinal plants against the structural proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), including the envelope, nucleocapsid, and spike/spike receptor-binding domain (RBD) proteins, in search of potential antiviral drug candidates. Computational analysis was used to screen for binding affinities and assess chemical interactions between ligands and target proteins. The findings revealed the top three potential compounds to bind to the envelope protein (cafestol, kahweol, and ledene), nucleocapsid protein (cafestol, kahweol, and thearubigin), spike protein (tannic acid, eugeniin, and kahweol), and spike RBD protein (kahweol, cafestol, and tannic acid). Moreover, the study identified four types of plants that contain potential bioactive compounds against SARS-CoV-2 structural proteins, including black tea (Camellia sinensis), clove (Syzygium aromaticum), common bean (Phaseolus vulgaris), and star anise (Illicium verum). Interestingly, kahweol exhibited possible binding activity against all four target proteins. This result suggests that bioactive compounds from the listed medicinal plants could potentially be developed into antiviral drugs against COVID-19.

A Green Approach: Syzygium aromaticum Mediated Synthesis of CuO Nanoparticles with Potential for Wastewater Treatment

A Green Approach: Syzygium aromaticum Mediated Synthesis of CuO Nanoparticles with Potential for Wastewater Treatment

Pseudomonas aeruginosa is an Effective Indicator for Screening of Quorum Sensing Inhibition by Plant Extracts

Quorum sensing is a well-known mechanism used by many bacteria to control important virulence factors through the production and subsequent response to N-acylhomoserine lactones (AHLs). Quorum sensing inhibition (QSI), targeting AHL-dependent signalling, has been reported as a strategy for the control of bacterial pathogenicity. Thus, this study aimed at investigating the capability of Pseudomonas aeruginosa as an indicator to rapidly screen for potential QSI caused by plant extracts based on pigment inhibition. Four ethanolic plant extracts including: dried flower buds of clove (Syzygium aromaticum L), bark of cinnamon (Cinnamomum cassia L), leaves of moringa (Moringa oleifera Lam), and Al-Gutub (Tribulus terrestris L) dried fruits were investigated for their QSI ability in comparison with furanone (the known QS inhibitor). Agar well diffusion assay was used, in which the indicator isolate, P. aeruginosa, was inoculated onto Pseudomonas special agar medium containing 8 mm wells to load different concentrations of the plant extracts along with furanone or phosphate buffered saline (negative control). The results showed that furanone was successful in inhibiting the pyocyanin pigment of P. aeruginosa without interfering with the bacterial growth, this verified the ability of this bacterium as a potent QS indicator organism. Importantly, clove extract at different concentrations caused complete inhibition of the pyocyanin pigment with little impact on the bacterial growth. On the use of cinnamon, only the high concentrations showed clear dye inhibition of the indicator organism. Moringa also caused certain degree of dye inhibition but only at high concentrations. Conversely, Al-Gutub neither affected on the pyocyanin production nor on the bacterial viability. In conclusion, P. aeruginosa isolate, pyocyanin producer, was successful in the screening of QS inhibitors. This simple study recommends the use of such isolate without relying on the import of certain strains of Chromobacterium violaceum, Pseudomonas aureofaciens, or Agrobacterium tumefaciens‎‎‎‎‎.