Garlic Seeds Research Articles

Evaluation of Iron Chlorin e6 disappearance and hydrolysis in soil and garlic using salting-out assisted liquid-liquid extraction coupled with high-performance liquid chromatography and ultraviolet-visible detection

Iron Chlorin e6 (ICE6), a star plant growth regulator (PGR) with independent intellectual property rights in China, has demonstrated its efficacy through numerous field experiments. We innovatively employed salting-out assisted liquid-liquid extraction (SALLE) with HPLC-UV/Vis to detect ICE6 residues in water, soil, garlic seeds, and sprouts. Using methanol and a C18 column with acetonitrile: 0.1% phosphoric acid mobile phase (55:45, v:v), we achieved a low LOQ of 0.43 to 0.77 μg kg−1. Calibration curves showed strong linearity (R2 > 0.992) within 0.01 to 5.00 mg kg−1. Inter-day and intra-day recoveries (0.05 to 0.50 mg kg−1) demonstrated high sensitivity and accuracy (recoveries: 75.36% to 107.86%; RSD: 1.03% to 8.78%). Additionally, density functional theory (DFT) analysis aligned UV/Vis spectra and indicated ICE6's first-order degradation (2.03 to 4.94 days) under various environmental conditions, mainly driven by abiotic degradation. This study enhances understanding of ICE6's environmental behavior, aids in risk assessment, and guides responsible use in agroecosystems.

Different Preharvest Diseases in Garlic and Their Eco-Friendly Management Strategies.

garlic reproduces mainly through clove planting, as sexual reproduction via seeds is uncommon. Growers encounter challenges with pathogens due to the larger size and vegetative nature of seed cloves, as well as the storage conditions conducive to fungal growth. Some Phyto-pathogenic fungi, previously unrecognized as garlic infections, can remain latent within bulb tissues long after harvest. Although outwardly healthy, these infected bulbs may develop rot under specific conditions. planting diseased seed cloves can contaminate field soil, with some fungal and bacterial infections persisting for extended periods. The substantial size of seed cloves makes complete eradication of deeply ingrained infections difficult, despite the use of systemic fungicides during the preplanting and postharvest phases. Additionally, viruses, resistant to fungicides, persist in vegetative material. They are prevalent in much of the garlic used for planting, and their host vectors are difficult to eliminate. To address these challenges, tissue-culture techniques are increasingly employed to produce disease-free planting stock. Key scientific concepts of the review: garlic faces a concealed spectrum of diseases that pose a global challenge, encompassing fungal threats like Fusarium's vascular wilt and Alternaria's moldy rot, bacterial blights, and the elusive garlic yellow stripe virus. The struggle to eliminate deeply ingrained infections is exacerbated by the substantial size of seed cloves. Moreover, viruses persist in garlic seeds, spreading through carrier vectors, and remain unaffected by fungicides. This review emphasizes eco-friendly strategies to address these challenges, focusing on preventive measures, biocontrol agents, and plant extracts. Tissue-culture techniques emerge as a promising solution for generating disease-free garlic planting material. The review advocates for ongoing research to ensure sustainable garlic cultivation, recognizing the imperative of safeguarding this culinary staple from an array of fungal and viral threats.

DETERMINAN PRODUKSI BENIH BAWANG PUTIH DI KECAMATAN SEMBALUN

This research aims to describe the characteristics of farmers and seed stockmen and analyze the determinants of garlic seed production in Sembalun District. The research was conducted through observations and interviews using questionnaires to farmers and garlic seed stockmen. The qualitative analysis method was used to identify the characteristics of farmers and seed stockmen and then quantitative analysis using multiple regression to identify the determinants of garlic seed production in Sembalun District. Based on the analysis conducted on 35 respondents (30 farmers and 5 seed stockmen) there were four variables that affect the production of garlic seeds in Sembalun District, namely wet garlic production (X1), the price of garlic seeds (X2), the price of wet garlic (X3), and the price of organic fertilizer (X4). Wet garlic production (X1) and the price of garlic seeds (X2) were found to have a positive effect while the price of wet garlic (X3) and the price of organic fertilizer (X4) had a negative effect on the production of garlic seeds in Sembalun District.

Parameter Optimization of Finger Clip Plate Garlic Seed-Metering Device

In order to improve the success rate of garlic sowing and the qualification rate of garlic seeds, a fingerboard-type garlic seed metering device was designed, and its parameters were optimized. First, the structure and working principles of seed-metering devices were studied. Subsequently, its critical component parameters were determined using theoretical calculations; then, discrete element method multi-body dynamics (DEM-MBD)-coupling simulation software was used to explore the seed-metering device process and analyze the effects of the opening angle of the clamping plate, the diameter of the seed scoop, and rotational speed of the seeding plate on the single-seed rate, missing rate, and reply rate of the device. Finally, a quadratic regression orthogonal rotation simulation experiment was conducted using the splint opening angle, scoop opening diameter, and rotation speed of the seeding plate as experimental factors, with the single-seed filling rate, qualified percentage, and missing rate as evaluation indicators. A three-factor, five-level orthogonal test was conducted to determine the mathematical regression model of experiment factors and evaluation indicators and to realize parameter optimization. A bench validation test was conducted using a 63° splint opening angle, a 24 mm seed-collecting scoop opening diameter, and 34 r/min seed-metering plate rotation speed. The average qualified rate, missing rate, and reply rate of single seeds were 91.86%, 2.71%, and 5.43%, respectively, which was consistent with the regression model results. This indicates that the method proposed in this paper achieved parameter optimization of a fingerboard garlic seed metering device.

Design and experiment of a type hole wheel garlic seed metering device

Aiming at the problems of low single-seed rate, high rate of missing seeds, high rate of multiple seeds, and low rate of upright seeds in the mechanized sowing of garlic seeds, this paper proposes a structural design scheme of a type hole wheel garlic seed metering device based on the characteristics of garlic species. Based on the agronomic requirements of garlic planting and the characteristics of garlic species, the mechanism of the hole wheel structure to improve the seed filling performance of the seed metering device was clarified, a structural parameter design of the key components of the device was established through theoretical calculations, and a single-factor simulation test was conducted using the Engineering Discrete Element Modelling (EDEM) software. Using the Design-Expert 11 data analysis software, a mathematical regression model of each test factor and test index was established, and the test factors were analyzed. The parameters were optimized, and the following was found to be the optimum parameter combination: seeding wheel speed of 30 rpm, profile hole depth of 18 mm, and adjustment length of the profile hole of 41 mm. The EDEM simulation test results showed a single-seed rate of 91.67% and a missing seed rate of 4.17%. The bench test results showed a single-seed rate of 89.2%, a missing seed rate of 5.0%, and a multiple seed rate of 5.8%. The field test results showed a single-seed rate of 88.44%, a multiple seed rate of 4.11%, a missing seed rate of 7.44%, a breakage rate of 1.11%, and an upright rate of 62.36%. The errors between the bench test/field test and the discrete element simulation test results were 2.7% and 3.5%, respectively, which were consistent with the simulation results, thus meeting the requirements of single-seed sowing using the garlic seed metering device.

Comparison of Different Physical Methods and Preservatives for Control of Fusarium proliferatum Rot in Garlic

Dry rot is an emerging issue for garlic production worldwide and Fusarium proliferatum is its major causal agent. Since the disease is seed-transmitted, sowing healthy cloves is crucial. In this study, some disinfection strategies were tested on garlic seeds, including steam, dry heat, chemical disinfectants and gaseous ozone (O3). Steam reduced the Colony Forming Units·g−1 (CFUs·g−1) by up to 92% in garlic seeds, but, at the same time, it affected their germination (−36%). Similarly, hydrogen peroxide (H2O2) and peracetic acid (C2H4O3) reduced the CFUs·g−1 by up to 83%; however, these methods also severely impaired germination (−40%). Dry heat did not negatively impact germination, but fungal contamination was not significantly reduced. The most promising strategy was gaseous O3 treatment; it decreased CFUs·g−1 by up to 96%, without causing any reduction of germination. The treatments applied were partially effective because the fungus is predominantly located in the outer layer of the seed, although it is also found in the inner portions. Some of these treatments can contribute to garlic protection from seed-borne pathogens and possibly reduce the occurrence of garlic dry rot.

Garlic Potyviruses Are Translocated to the True Seeds through the Vegetative and Reproductive Systems of the Mother Plant.

Garlic lost its ability to produce true seeds millennia ago, and today non-fertile commercial cultivars are propagated only vegetatively. Garlic viruses are commonly carried over from one generation of vegetative propagules to the other, while nematodes and arthropods further transmit the pathogens from infected to healthy plants. A recent breakthrough in the production of true (botanical) garlic seeds resulted in rapid scientific progress, but the question of whether viruses are transmitted via seeds remains open and is important for the further development of commercial seed production. We combined morpho-physiological analysis, fluorescence in situ hybridization (FISH), and PCR analysis to follow potyvirus localization and translocation within garlic fertile plants and seeds. Spatial distribution was recorded in both vegetative and reproductive organs. We conclude that garlic potyviruses are translocated to the seeds from the infected mother plant during flower development and post-fertilization, while pollen remains virus-free and does not contribute to seed infection. Therefore, the main practical goal for virus-clean seed production in garlic is the careful maintenance of virus-free mother plants. Although garlic pollen is free of potyviral infection, the male parents’ plants also need to be protected from contamination, since viral infection weakens plants, reducing flowering ability and pollen production.

Contour Resampling-Based Garlic Clove Bud Orientation Recognition for High-Speed Precision Seeding

Achieving fast and accurate recognition of garlic clove bud orientation is necessary for high-speed garlic seed righting operation and precision sowing. However, disturbances from actual field sowing conditions, such as garlic skin, vibration, and rapid movement of garlic seeds, can affect the accuracy of recognition. Meanwhile, garlic precision planters need to realize a recognition algorithm with low-delay calculation under the condition of limited computing power, which is a challenge for embedded computing platforms. Existing solutions suffer from low recognition rate and high algorithm complexity. Therefore, a high-speed method for recognizing garlic clove bud direction based on deep learning is proposed, which uses an auxiliary device to obtain the garlic clove contours as the basis for bud orientation classification. First, hybrid garlic breeds with the largest variation in shape were selected randomly and used as research materials, and a binary image dataset of garlic seed contours was created through image sampling and various data enhancement methods to ensure the generalization of the model that had been trained on the data. Second, three lightweight deep-learning classifiers, transfer learning based on MobileNetV3, a naive convolutional neural network model, and a contour resampling-based fully connected network, were utilized to realize accurate and high-speed orientation recognition of garlic clove buds. Third, after the optimization of the model’s structure and hyper-parameters, recognition models suitable for different levels of embedded hardware performance were trained and tested on the low-cost embedded platform. The experimental results showed that the MobileNetV3 model based on transfer learning, the naive convolutional neural network model, and the fully connected model achieved accuracy of 98.71, 98.21, and 98.16%, respectively. The recognition speed of the three including auxiliary programs was 19.35, 97.39, and 151.40 FPS, respectively. Theoretically, the processing speed of 151 seeds per second achieves a 1.3 hm2/h planting speed with single-row operation, which outperforms state-of-the-art methods in garlic-clove-bud-orientation recognition and could meet the needs of high-speed precise seeding.

The Utilization of Off Grade Garlic Bulb Into Garlic Powder and Its Characteristics

During the production process of garlic seeds, about 15 - 20% are not suitable for seed use because of the small size of the cloves (<2.0 cm). Garlic powder has a potential to be developed as a seasoning in cooking, medicine, and industrial raw material. This research aimed to determine the effect of size reduction method and sodium bisulphite concentration on the characteristics of garlic powder from off-grade garlic. The raw material used was off grade garlic of Sangga Sembalun variety. The study was designed using a completely randomized design, which consisted of two factors, namely the size reduction method (x1: chopped and x2: sliced) and concentration of sodium bisulphite solution (y1: 0, y2: 0.5%, y3: 1.0%; y4: 1.5%), with three replication. The results showed that off grade garlic can be used as garlic powder with high lightness and allicin content. The garlic powder produced by the slice method and soaking in 0.5% of sodium bisulphite had a yield of 39.83%, moisture of 8.37%, ash of 3.47%, fat of 0.21%, protein of 18.30%, carbohydrate of 69.63%, allicin of 1.59%, chroma of 13.80, degree of Hue of 71.04, and degree of Lightness (L*) of 89.56.

PARTNERSHIP OF GARLIC FARMERS AND IMPORTERS IN KARANGANYAR REGENCY

Garlic planting program is the implementation of Republic of Indonesia Minister of Agriculture’s Regulation Number 16 of 2017 about Horticultural Product Import Recommendation (RIPH). This garlic planting program was conducted by importer along with partner farmer group in Sepanjang and Nglebak Villages, Karanganyar Regency in 2018. The objectives of research are to describe the implementation of Republic of Indonesia Minister of Agriculture’s Regulation Number 16 of 2017 in the program conducted by importer in Karanganyar Regency and to study the implementation of garlic planting partnership program conducted by importer based on CIPP evaluation. The research method used was descriptive qualitative one. The result of research shows that the implementation of Republic of Indonesia Minister of Agriculture’s Regulation Number 16 of 2017 by importer along with farmer group in Sepanjang and Nglebak Villages was conducted with 128 participants and 18.1 Ha land width. Evaluation on the garlic planting partnership program between importer and farmers in Sepanjang and Nglebak Villages using CIPP does not achieve result as targeted. The implementation of garlic planting is not as planned. Poor quality of garlic seeds and planting period incompatible to the condition of Tawangmangu Sub District area made this program failed and unsustainable.

Productivity and Quality of Garlic Produced Using Below-Zero Temperatures When Treating Seed Cloves

Garlic cultivation has increased in Brazil in recent years primarily due to the adoption of appropriate technologies, such as the use of low temperatures during the maintenance of garlic seeds to overcome dormancy. However, there is no information on the effects of below-zero temperatures when treating seed cloves on garlic development. Therefore, this study’s objective was to evaluate the effects of below-zero temperatures and different visual indices of overcoming dormancy (VIDs) on garlic performance in Cristalina County, Goias State, Brazil. The experiment was conducted in a randomized block design with four replicates in a 2 × 3 factorial scheme: with two VIDs (40% and 60%), and three temperature ranges (−1 to −3 °C, 1 to 3 °C, and 2 to 4 °C). Vegetative characteristics, bulbar ratios, and commercial bulb yields were evaluated. The results showed that below-zero temperatures resulted in better vegetative characteristics. The yield increased after using below-zero temperatures to treat seed cloves with a VID of 60%. The garlic produced had a higher market value. We concluded that there is an enormous potential for using below-zero temperatures to improve the performance of the “Ito” garlic variety, and more studies should be conducted with other varieties of economic importance to enhance Brazilian garlic production.

The effect of NAA and coconut water combination on garlic (Allium sativum L.) tissue culture

Tissue culture techniques can increase the number of garlic seedlings. The purpose of this research is to determine the effect of NAA and coconut water in increasing the number of garlic seeds. This research used a Completely Randomized Design of two factors. The treatment used is NAA with concentrations of 0 ppm, 0.5 ppm, 1 ppm, 1.5 ppm, and coconut water concentrations of 0%, 10%, 20%. The variables observed were shoot emergence time, root emergence time, number of shoots, number of roots, number of leaves, shoot height, root length, and number of plantlets. The results showed that the addition of coconut water 20% without the addition of NAA in 1 bulb can produce 3.33 planlets and the results of explant propagation in 1 bulb can produce the number of shoots as many as 15.33 shoots. Giving coconut water with concentrations of 10% and 20% can increase the number of leaves, shoot height, and some planlets. The concentration of NAA 0.5 ppm can accelerate the root emergence time on garlic explant.

Double Infection of Onion yellow dwarf virus and Shallot latent virus in Garlic from Several Regions in Indonesia

Viruses have been a problem on garlic cultivations in various countries. There are several viruses reported infecting garlic. Genera Potyvirus and Carlavirus are the most common viruses found infecting garlic. Mixed infection on garlic is often designated as a “garlic viral complex”. These viruses can be transmitted through imported garlic seeds. Therefore, it is necessary to conduct early detection of garlic seeds to prevent the epidemic of these viruses. This study aimed to detect Onion yellow dwarf virus (OYDV) and Shallot latent virus (SLV) on garlic. Garlic samples were obtained from Enrekang, Magelang, Temanggung, Tawangmangu, and Yogyakarta. Total RNA was extracted from the samples and subsequently used for RT-PCR using two pairs of specific primers SLV-F/SLV-R and OYDV-F/OYDV-R. Primary pair SLV-F/SLV-R in amplicons sized 276 bp, while OYDV-F/OYDV-R in amplicons sized 112 bp. RT-PCR results showed that OYDV was found in all samples tested in this study. Meanwhile, double infections (OYDV and SLV) were found in eight out of ten samples tested. These results indicated that double infections on garlic were common in Indonesia.

Degenerescence of virus-free garlic cultivars in altitude conditions in the Semiarid region of the Northeast of Brazil

ABSTRACT It is necessary to use virus-free garlic seeds to reach high bulb yields; however, information is needed on the number of generations that these seeds can be grown in field conditions without decreasing bulb yield and quality due to degeneration. Degeneration is dependent on the production season and locality; thus, the objective of this work was to evaluate the degeneration of virus-free garlic cultivars grown in the Semiarid region of the Northeast of Brazil, through three experiments conducted in Portalegre, state of Rio Grande do Norte. Virus-free garlic cultivars were used in the first generation (G1) and second-generation (G2) and cultivars without clonal cleaning (infected) in the third experiment. The experiments were conducted in randomized blocks experimental design, with four replications. The treatments consisted of five cultivars: Amarante, Branco Mossoró, Cateto Roxo, Gravatá, and Hozan. The use of G2 garlic seeds in field conditions is viable, mainly for the cultivars Branco Mossoró and Hozan, which showed lower production losses. However, further evaluations, considering at least four generations, are necessary for these cultivars, since the degeneration level depends on the crop environment.

Performance Evaluation of Tractor Operated Garlic (Allium sativum L.) Clove Planter

Garlic (Allium sativum L.) is an important foreign exchange earner for India. The farmers generally plant garlic by manual method, which is labour intensive and time consuming. A tractor-operated garlic clove planter was developed and evaluated for its performance in the field. The average wheel slip, draft and average depth of seed placement by the developed planter were 6.93 %, 289.83 kg, and 42.1 mm, respectively. The average seed spacing, miss index, multiple index, quality of feed index, seed rate and seed metering efficiency for GG-4 and GG-5 garlic varieties were 99.4 mm, 6.12 %, 7.91 %, 85.96 %, 377 kg.ha-1; and 96.35 % and 99.9 mm, 4.58 %, 6.07 %, 89.34 %, 443 kg.ha-1, and 96.75 %, respectively during field tests. The effective field capacity and field efficiency of the developed planter were 0.33 ha.h-1 and 80.33; 0.32 ha.h-1 and 79.02 %, respectively, for GG-4 and GG-5 garlic seeds. The cost of planting by the developed planter with operational cost of tractor was ` 553.63 per hour (` 1677.67 per ha), and was less as compared to available planting machineries for garlic.

The dormancy breaking of garlic seeds through thermal shock storage methods and soaking in gibberellin acid

Garlic seed production is constrained by a long dormancy period, which is 5-6 months. This study aimed to analyses the effect of thermal shock storage methods and soaking in gibberellin acid on the dormancy breaking of garlic seeds. Raw material used in this study was garlic bulbs variety Sangga Sembalun. The experiment was conducted in randomized block design with treatments of storage temperature (low temperature / 12-14°C, high temperature / 38-42°C + low temperature / 12-14°C, high temperature / 38-42°C + room temperature / 28-30°C, and room temperature) and gibberellin acid concentration (0, 125, 250, 375 ppm), with two replications. Total storage duration was 4, 8, and 12 weeks. The results of analysis of variance (ANOVA) showed that storage temperature was significantly different of their germination (p<0.05). Storage of garlic seeds by the thermal shock method can shorten the dormancy period from 5-6 months to 12 weeks. Germination capacity resulting from combination of high temperature (38-42°C) storage for 6 weeks and low temperature (12-14°C) for 6 weeks (a total of 12 weeks) is higher than continuous storage of low temperature and room temperature without requiring the addition gibberellin acid.

THE EFFECT OF CORONA INCANDESCENT PLASMA RADIATION AND STORAGE TEMPERATURE ON THE VIABILITY OF GARLIC SEEDS (ALLIUM SATIVUM L. LUMBU KUNING VARIETY)

Garlic (Allium sativum L.) is an agricultural commodity which is widely consumed by the community. The obstacle experienced in cultivation is that a relatively long dormancy period of about 6-7 months. The dormancy of garlic seeds occurs because the embryo has not yet been fully formed, and the growth hormone and inhibitory hormone are not balanced. The methods used to break dormancy and accelerate germination are by using corona incandescent plasma radiation and storage temperature. The research utilized factorial Completely Randomized Design (CRD), consisting of six treatments with five replications. The first factor was the corona incandescent plasma radiation carried out on the garlic seeds ‘Lumbu Kuning’ variety with time duration of 0, 15, and 30 minutes. The second factor was storage temperature comprising of room temperature and cold temperature of 7oC stored for 30 days. Viability parameters observed are germination percentage dan the length of sprouts. The data were analyzed by using ANOVA followed by the DMRT test at the 95% confidence level. The results showed that corona incandescent plasma radiation influenced the germination percentage using plasma radiation for 15- 30 minutes.

ВЛИЯНИЕ РАСТИТЕЛЬНЫХ ЭКСТРАКТОВ НА УРОВЕНЬ СВОБОДНО РАДИКАЛЬНЫХ ПРОЦЕССОВ В КЛЕТКАХ ЧЕЛОВЕКА

We evaluated the effects of extracts from the pomegranate (Punica granatum), vine (Vitis vinifera) seeds and garlic (Allium sativum) on free-radical reactions in human cells in relation to SNPs in the SOD2and СAT genes. The object was human peripheral leukocytes cultured in the presence of extracts from pomegranate (1.2; 2.4 %), garlic (0.5; 1.2 %) and vine seeds (1.2; 2.4 %). Intensity of free-radical reactions was assessed by a fast flash and flashlight sum of the luminol-dependent chemiluminescence (LDCL). Alleles of SOD2and СAT were analyzed using allele-specific PCR. The results showed that the pomegranate extract decreases LDCL intensity reliably in comparison with the control. Extracts of the garlic (1.2 %) and vine seeds (1.2 %) increase LDCL intensity. Correlation was established between the genotype determined by polymorphism of SOD2 gene Ala16Val (rs4880) and fast flash intensity during human cell cultivation with the extract of vine seeds (1.2 %).

Empowering certified garlic seed producers to increase local production in Sembalun highlands of Eastern Lombok, Indonesia

Garlic demand in Indonesia reaches 500,000 tons yearly and 82.5% supplied from importation. This problem could be addressed by increasing national production. However, garlic production is very dependent on seeds availability, therefore efforts to produce high quality seeds is a must. One of the efforts is through developing certified garlic seed producers. This research aims to study efforts by Assessment Institute for Agricultural Technology (AIAT) West Nusa Tenggara Province, Indonesia in empowering growth of new certified seed producers in Lombok Island. Data obtained through in-depth interviews with existing seed producers, new seed producers, community leaders and garlic farmers during 2017-2018. The results showed that formation of two new certified seed producers could increase the coverage of farmers who are assisted from the previous 10 to 20 farmers per group who have a commitment to work together to sell their garlic seeds. Seed producers can be a communication channel that increases farmers' knowledge on garlic cultivation because of an increasingly extensive network and sources of information. Supervising process to new certified seed producers enabling them to produce high quality seeds. In addition, certified garlic seeds produced are easier to recognize by buyers and able to penetrate national market with competitive prices.

Performance Evaluation of Tractor Operated Garlic (Allium sativum L.) Clove Planter

Garlic (Allium sativum L.) is an important foreign exchange earner for India. The farmers generally plant garlic by manual method, which is labour intensive and time consuming. A tractor-operated garlic clove planter was developed and evaluated for its performance in the field. The average wheel slip, draft and average depth of seed placement by the developed planter were 6.93 %, 289.83 kg, and 42.1 mm, respectively. The average seed spacing, miss index, multiple index, quality of feed index, seed rate and seed metering efficiency for GG-4 and GG-5 garlic varieties were 99.4 mm, 6.12 %, 7.91 %, 85.96 %, 377 kg.ha-1; and 96.35 % and 99.9 mm, 4.58 %, 6.07 %, 89.34 %, 443 kg.ha-1, and 96.75 %, respectively during field tests. The effective field capacity and field efficiency of the developed planter were 0.33 ha.h-1 and 80.33; 0.32 ha.h-1 and 79.02 %, respectively, for GG-4 and GG-5 garlic seeds. The cost of planting by the developed planter with operational cost of tractor was ` 553.63 per hour (` 1677.67 per ha), and was less as compared to available planting machineries for garlic.