Garlic Essential Oil Research Articles

Physicochemical comparison of chitin characteristics in three major stored-product beetle pests: Implications for biofumigant toxicity

The present study investigates the chitin properties of stored-product insect pests and their association with the fumigant toxicity of garlic essential oil. Chitin isolates of Callosobruchus maculatus, Sitophilus oryzae, and Tribolium castaneum adults were characterized using FT-IR, XRD, EA, SEM-EDS, and NMR techniques. Fumigant toxicity assay was performed under airtight condition in glass vial. The S. oryzae contains highest chitin content (19 %), followed by T. castaneum (10 %) and C. maculatus (8 %). The degree of crystallinity was lower in C. maculatus (67.13 %) than in S. oryzae (77.05 %) and T. castaneum (76.56 %). Morphologically, C. maculatus chitin displayed a flat lamellar surface with pores, while S. oryzae and T. castaneum exhibited densely arranged microfibrils based surfaces. Fumigant toxicity assays revealed varied susceptibility levels, C. maculatus exhibited higher susceptibility (0.27 μL/L air of LC50) compared to S. oryzae and T. castaneum (14.35 and 3.74 μL/L air of LC50, respectively) to garlic essential oil. The higher chitin content, greater crystallinity, and densely arranged structures in S. oryzae might contribute to its tolerance towards fumigant. Additionally, physico-chemical properties and penetration potentiality of the bioactive constituents might be linked to the toxicity in insects. Understanding these relations can enrich knowledge of chitin's role in fumigant toxicity mechanism.

Influence of Different Plant Extracts on CYP-Mediated Skatole and Indole Degradation in Pigs.

One of the primary substances responsible for the unpleasant odor in boar meat is skatole. Enzymes belonging to the cytochrome P450 (CYP) family play a pivotal role in the hepatic clearance of skatole. This study aimed to investigate the impact of oregano essential oil (OEO), Schisandra chinensis extract (SC), and garlic essential oil (GEO) on hepatic CYP2E1 and CYP2A activity in pigs. In three consecutive trials, cannulated castrated male pigs were provided with a diet containing 0.2-0.3% of one of these plant extracts. Following a 14-day feeding period, the animals were slaughtered, and liver and fat samples were collected. The findings indicate that the activities of CYP2E1 were unaffected by any treatment. However, GEO treatment demonstrated a significant reduction in CYP2A activity (p < 0.05). Pigs treated with GEO also exhibited a notable increase in skatole concentrations in both plasma and adipose tissue. In contrast, animals fed SC displayed elevated skatole concentrations in plasma but not in fat tissue. OEO did not influence skatole concentrations in either blood or fat. Furthermore, the study revealed that a supplementation of 6 g GEO per animal per day induced a significant increase in skatole concentrations in blood plasma within 24 h.

Evaluating the hypolipidemic effect of garlic essential oil encapsulated in a novel double-layer delivery system

The limited application of garlic essential oil (GEO) is attributed to its pungent taste, poor water solubility and low bioavailability. Liposomes are nontoxic, biodegradable and biocompatible, and β-cyclodextrin can inhibit undesirable odors and improve the stability and bioavailability. Thus a promising dual-layer GEO β-cyclodextrin inclusion compound liposome (GEO-DCL) delivery system with both advantages was designed and prepared in this study. Experimental results indicated that the encapsulation efficiency of GEO-DCLs was 5% higher than that of GEO liposomes (GEO-CLs), reaching more than 88%. In vitro release experiment showed that the release rate of GEO in GEO-DCLs was 40% lower than that of GEO-CLs after incubation in gastric juice for 6-h, indicating that the stability of GEO-DCLs was better than GEO-CLs. Evaluation of the effects of GEO-DCLs on lowering blood lipid levels in hypercholesterolemia mice. GEO-DCLs could reduce the weight and fat deposition in hypercholesterolemia mice. Inhibiting the increase of TC, LDL-C, and decrease of HDL-C in mice. The degree of liver injury was decreased, the number of round lipid droplets in liver cytoplasm was reduced, and the growth of fat cells was inhibited. The lipid-lowering effects of GEO-DCLs were dose-dependent. GEO-DCL can improve the bioavailability of GEO and improve dyslipidemia. Based on GEO's efficacy in lowering blood lipids, this study developed a kind of GEO-DCL compound pomegranate juice beverage with good taste, miscibility and double effect of reducing blood lipids. This study lays a foundation for the application of GEO in the field of functional food.

Bacteriolytic activity of ruminal protozoa is affected by rate and type of common essential oils: Effect of thyme oil

Over the past two decades, research into the effects of essential oils (EOs) on rumen microorganisms as an alternative to antibiotics has intensified. Yet, there are few investigations on rumen protozoa. To evaluate if EOs have antiprotozoal efficacy against rumen protozoa, the present investigation was conducted. Using the potentiality of rumen protozoa to digest 14C-labeled bacteria, the influence of EOs on in vitro protozoal activity was determined. For the study, orange peel, cinnamon, laurel, oleaster, garlic, and thyme essential oils were selected. Control (without essential oil), 500 mg/kg, 1000 mg/kg, and 5000 mg/kg dosages were used. Except for garlic oil, all essential oils showed a notable effect on antiprotozoal activity. The highest antiprotozoal impact was reported at 500 mg/kg and 1000 mg/kg doses of thyme oil, with respective reductions of 89.1% and 84.8% in bacteria degraded by protozoa. Cinnamon and oleaster oils had the strongest antiprotozoal efficacy at a concentration of 5000 mg/kg. At 5000 mg/kg doses of oleaster and cinnamon oils, the quantity of 14C-labeled bacteria degraded by protozoa was reduced by 96% compared to the group that served as the control. In conclusion, orange peel, cinnamon, laurel, oleaster, and thyme oils demonstrated remarkable in vitro antiprotozoal activity; however, more comprehensive research is required to analyse the effects of the EOs used in this study on rumen living groups such as protozoa, bacteria, and yeast and on rumen dynamics.

Antibacterial, anti-biofilm activity and underlying mechanism of garlic essential oil in water nanoemulsion against Listeria monocytogenes

Garlic essential oil (GEO) in water nanoemulsion (GEON) has been reported to possess good stability and broad-spectrum antibacterial activity, but its effect on Listeria monocytogenes is rarely studied. In the present study, the antibacterial, anti-biofilm formation activity and underlying mechanism of GEON against L. monocytogenes were investigated. Results showed that GEON (mean particle size = 136.43 nm) significantly inhibited the growth of planktonic L. monocytogenes, and the minimum inhibitory concentration (MIC) of GEO in the nanoemulsion was 512 μg/mL. GEON could effectively kill L. monocytogenes in a time- and concentration-dependent manner. After GEON treatment, the cell membrane was severely damaged, which was proven by remarkable membrane depolarization and considerable loss of intracellular substances (nucleic acid, protein, and ATP). Moreover, decreased membrane integrity and collapsed cell morphology further confirmed the cell membrane damage. Additionally, GEON significantly attenuated the biofilm formation, enhanced the surface hydrophobicity, and reduced the swimming motility of L. monocytogenes. These results indicate that GEON has the potential to inhibit the planktonic growth and biofilm formation of L. monocytogenes.

A Review of Potential Feed Additives Intended for Carbon Footprint Reduction through Methane Abatement in Dairy Cattle.

Eight rumen additives were chosen for an enteric methane-mitigating comparison study including garlic oil (GO), nitrate, Ascophyllum nodosum (AN), Asparagopsis (ASP), Lactobacillus plantarum (LAB), chitosan (CHI), essential oils (EOs) and 3-nitrooxypropanol (3-NOP). Dose-dependent analysis was carried out on selected feed additives using a meta-analysis approach to determine effectiveness in live subjects or potential efficacy in live animal trials with particular attention given to enteric gas, volatile fatty acid concentrations, and rumen microbial counts. All meta-analysis involving additives GO, nitrates, LAB, CHI, EOs, and 3-NOP revealed a reduction in methane production, while individual studies for AN and ASP displayed ruminal bacterial community improvement and a reduction in enteric CH4. Rumen protozoal depression was observed with GO and AN supplementation as well as an increase in propionate production with GO, LAB, ASP, CHI, and 3-NOP rumen fluid inoculation. GO, AN, ASP, and LAB demonstrated mechanisms in vitro as feed additives to improve rumen function and act as enteric methane mitigators. Enzyme inhibitor 3-NOP displays the greatest in vivo CH4 mitigating capabilities compared to essential oil commercial products. Furthermore, this meta-analysis study revealed that in vitro studies in general displayed a greater level of methane mitigation with these compounds than was seen in vivo, emphasising the importance of in vivo trials for final verification of use. While in vitro gas production systems predict in vivo methane production and fermentation trends with reasonable accuracy, it is necessary to confirm feed additive rumen influence in vivo before practical application.

The Effect of Casein-Chitosan Edible Coating with Garlic Essential Oil Addition on pH, Haugh Unit and Egg Yolk Index Fresh Chicken Egg on Different Storage

Eggs are a source of animal protein needed by the body and contain complete essential amino acids. Chicken eggs are easily damaged or changed so the quality of the eggs decreases with the length of storage. This damage can be prevented by coating. Past studies concluded that edible coating can maintain the quality of chicken eggs. The purpose of this study was to determine the effect of coating chicken eggs using chitosan and casein edible coating with the addition of garlic essential oil. The materials used are fresh chicken eggs, edible coating solution and garlic essential oil. The method used in this study was a laboratory experiment with Completely Randomized Nested Design with 2 treatments and 3 replications. Edible coating with the addition of garlic essential oil is expected to improve egg quality with a long shelf life. The result showed that chicken eggs coated with chitosan casein edible coating with the addition of garlic essential oil 1% for storage time of 0 days, 7 days, 14 days, 21 days and 28 days gave a very significant effect (P&lt;0,01) on egg yolk pH, egg albumen pH, haugh unit and egg yolk index.

Effects of turmeric, ginger, cinnamon, and garlic essential oils on HSP70, NFκB, oxidative DNA damage, inflammatory cytokines, and oxidative markers in broiler chickens.

In recent years, the use of natural bioactive compounds derived from spices has garnered significant interest in poultry production due to their potential to modulate immune responses and oxidative stress. An investigation into the roles of spices essential oils (EO) on inflammatory cytokines, HSP70 and oxidative markers of broiler chickens was conducted in this study. Four spices consisting of garlic, ginger, turmeric, and cinnamon were processed to obtain their respective EO. Two hundred 1-d-old arbo acre broilers were allotted to 5 treatments consisting of B1 (control), B2 (garlic EO), B3 (ginger EO), B4 (Turmeric EO), and B5 (cinnamon EO), with EOs administered to drinking water at 30% (v/v) in a 49-d trial. Blood was sampled for assessment of hematological parameters, and serum obtained were assayed for inflammatory cytokines, antioxidant activities, nuclear factor kappa B (NFκB), 8-hydroxydeoxyguanosine (8-OHdG), and heat shock protein 70 (HSP70) levels using standard procedures. Results obtained revealed that cinnamon EO enhanced erythrocytic indices, leukocyte profile, catalase, glutathione peroxidase and interleukin 10, lowers interleukin 1 beta (IL-1β) and interferon gamma (IFN-γ), enhanced HSP70 and higher 8-OHdG levels in chicken. Garlic EO enhanced monocytes and superoxide dismutase, while reduced IFN-γ and HSP70, but increased IL-1β and tumor necrosis factor alpha (TNF-α) NFκB in broiler chickens. Ginger EO also enhanced erythrocytic indices, total antioxidant activity, lowered IFN-γ and lipid peroxidation, while turmeric EO enhanced total antioxidant activity, catalase and lowered IFN-γ and increased 8ohdg in broiler chickens. In conclusion, this study revealed that ginger and turmeric EO were more beneficial in preventing oxidative DNA damage, cinnamon EO enhanced serum oxidative status and lowered pro-inflammatory cytokines, while garlic EO reduced HSP70 in broiler chickens.

Thyme, Oregano, and Garlic Essential Oils and Their Main Active Compounds Influence Eimeria tenella Intracellular Development.

Coccidiosis poses a significant challenge in poultry production and is typically managed with ionophores and chemical anticoccidials. However, the emergence of drug resistance and limitations on their use have encouraged the exploration of alternative solutions, including botanical compounds and improvements in in vitro screening methods. Prior research focused only on the impact of these alternatives on Eimeria invasion, with intracellular development in cell cultures receiving limited attention. This study assessed the impact of thyme (Thymus vulgaris), oregano (Origanum vulgare), and garlic (Allium sativum) essential oils, as well as their bioactive compounds, on the initial phase of schizogony in Madin-Darby bovine kidney cells, comparing their effectiveness to two commercially used anticoccidial drugs. Using image analysis and quantitative PCR, the study confirmed the efficacy of commercial anticoccidials in reducing invasion and schizont formation, and it found that essential oils were equally effective. Notably, thymol and carvacrol exhibited mild inhibition of intracellular replication of the parasite but significantly reduced schizont numbers, implying a potential reduction in pathogenicity. In conclusion, this research highlights the promise of essential oils and their bioactive components as viable alternatives to traditional anticoccidial drugs for mitigating coccidiosis in poultry, particularly by disrupting the intracellular development of the parasites.

Garlic essential oil confers shielding against nephrotoxicity elicited by lead nitrate in Swiss albino mice

Lead is an immensely poisonous metal that can infiltrate the human body through various natural processes and human activities, therefore it possesses a significant risk to human health. Garlic (Allium sativum), a widely recognized medicinal plant, is employed to diminish a diverse array of health issues. While investigating the potential curative properties of the garlic essential oil (GEO) derived from fresh garlic bulbs, researchers explored its impact on the mice renal tissue subjected to lead nitrate. In the present research work, a sum of 36 healthy male Swiss albino mice were randomized into one control group (I) and five treatment groups: lead nitrate (II a), lead nitrate + low dose of GEO (II b), lead nitrate + high dose of GEO (II c), lead nitrate + silymarin (II d) and lead nitrate + vehicle olive oil (II e). Lead nitrate exposure resulted in elevated levels of alanine transferase (ALT), aspartate aminotransferase (AST), lipid peroxidation (LPO) and decreased levels of antioxidant enzymes, thus contributing to the oxidative stress and adversely affected the normal structure of renal tissues. Conversely, treatment with garlic essential oil (GEO) resulted in upsurge in these antioxidant levels and depletion in ALT, AST, and LPO levels. The findings support the notion that a higher dosage of garlic essential oil is more effective in mitigating lead nitrate-induced nephrotoxicity than a lower dosage. Consequently, garlic essential oil holds promise as a novel therapeutic agent for alleviating nephrotoxicity induced by lead nitrate exposure.

Assessing the In Vitro and In Vivo Effect of Supplementation with a Garlic (Allium sativum) and Oregano (Origanum vulgare) Essential Oil Mixture on Digestibility in West African Sheep.

This study assessed the impact of a mixture of garlic (Allium sativum) and oregano (Origanum vulgare) essential oils (EOGOs) on in vitro dry matter digestibility (IVDMD) and in vivo apparent nutrient digestibility. Different EOGO inclusion levels were evaluated to assess the dose response and potential effects of the mixture. Three EOGO inclusion levels (0.5, 0.75, and 1 mL/kg of incubated dry matter) were evaluated in vitro, while four treatments (0.5, 0.75, and 1 mL/day of EOGO and a control group) were tested in vivo on 12 West African sheep. A randomized controlled trial was conducted using a 4 × 4 design. Blood parameters (glucose, blood urea nitrogen, and β-hydroxybutyrate) were measured to observe the effect of EOGO on the metabolism. The results showed that the inclusion of EOGO significantly enhanced IVDMD at low levels (p < 0.052) compared with the highest levels in treatments containing 0.5 and 0.75 mL/kg of EOGO dry matter. A higher intake of dry matter (DM), crude protein (CP), and neutral detergent fiber (NDF) (p < 0.05) was observed in the in vivo diets with the inclusion of EOGO. In terms of in vivo apparent digestibility, significant differences were found among treatments in the digestibility coefficients of DM, CP, and NDF. EOGO inclusion increased the digestibility of DM. CP digestibility displayed a cubic effect (p < 0.038), with the lowest values of digestibility observed at 1 mL EOGO inclusion. Additionally, NDF digestibility showed a cubic effect (p < 0.012), with the highest value obtained at 0.75 mL of EOGO inclusion. The inclusion levels above 0.75 mL EOGO showed a cubic effect, which indicates that higher concentrations of EOGO may not be beneficial for the digestibility of CP and NDF. Although no significant difference was observed in total digestible nutrients, a linear trend was observed (p < 0.059). EOGO improved the intake of DM, CP, and NDF. EOGO supplementation improved the digestibility of DM and NDF, with optimal levels observed at 0.5 mL/day. No significant effects were observed in the blood parameters. These results suggest that EOGO has the potential as an additive in ruminal nutrition to improve food digestibility and serve as an alternative to antibiotic additives. The use of EOGO potentially improves fiber digestion and may reduce the use of antibiotics in livestock production. Garlic (A. sativum) and oregano (O. vulgare) essential oils effectively modulated fiber digestibility at 0.75 mL/day. Garlic (A. sativum) and oregano (O. vulgare) essential oils have the potential to improve digestibility at low inclusion levels and serve as an alternative to antibiotic additives. The effectiveness of essential oils is greater in a mixture and at lower doses.

Garlic and peppermint essential oils elicit plant defensive responses in sweet peppers

Garlic and peppermint essential oils elicit plant defensive responses in sweet peppers

Bioactivity of Allium sativum essential oil-based nano-emulsion against Planococcus citri and its predator Cryptolaemus montrouzieri

Botanical extracts, in particular essential oils (EOs), could be the ideal candidates for the development of biopesticides as an alternative to synthetic pesticides. However, some limitations of EOs (high flammability, volatility, degradability, poor solubility in water) prevent their use under real operational conditions. Nanotechnologies are useful tools to overcome the above-mentioned limitations of these natural substances. Furthermore, encapsulation in nano-delivery systems (nanoparticles and nano-emulsions) can improve the functional properties of EOs. In this context, this study aimed to develop a highly stable, concentrated garlic nano-emulsion (15%) and to evaluate the acute toxicity with different exposure routes towards Planococcus citri and its predator Cryptolaemus montrouzieri. First, garlic EO was used to develop a nano-emulsion (15% EO; 5% Tween 80; 80% water) using a high-pressure microfluidizer; then both the crude EO and EO in nano-emulsion were chemically investigated by Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry (SPME-GC-MS) technique. The nano-emulsion was physically characterized by dynamic light scattering analysis over time (24 h, 3 months and 1 year after preparation) and used in bioassays involving both the target and non-target organisms. Results showed that the garlic EO consisted of over 95% sulphur compounds with diallyl disulphide as the most abundant component, and the developed nano-emulsion remained stable even after 1 year, with droplets’ dimension within the nanometric range (221.4 nm). The nano-formulation was effective against the target pest after 48 h from the treatment (Direct: LC90 = 0.967%; Indirect: LC90 = 1.088%), while it had no effect on C. montrouzieri. These promising results highlight the potential of garlic-based nano-emulsion as effective and environmentally friendly insecticide for pest control.

Incorporation of garlic (Allium sativum) essential oil into cellulose acetate films: effect on the preservation of sliced cooked ham

AbstractGarlic essential oil (GEO) has been recognized as a natural antimicrobial agent with a broad spectrum of action that can be associated with polymeric sustainable matrices to provide active packaging capable of improving the preservation of ready‐to‐eat meat products. In this sense, the present study aimed to evaluate the effect of cellulose acetate (CA) films incorporated with GEO on the growth of spoilage microbial groups (psychrotrophic (PSY) and lactic acid bacteria (LAB)) and physicochemical characteristics (pH, acidity index, color and index of thiobarbituric acid) of vacuum‐packed cooked sliced ham. Four treatments were evaluated (no film, CA‐film without GEO, CA‐film with 1.5% of GEO and CA‐film with 3% of GEO, applied as interfold packaging) over time (12 days). There was a significant reduction (ca 2.0 log cycle) in PSY and LAB growth when the active films were applied between the ham slices. Also, the lag phase of PSY was extended from around 2 to 6 days, which might positively impact the product's shelf life. However, the active films showed no significant effect on the food physicochemical characteristics. Therefore, the active CA‐films incorporated with small amounts of GEO could be an interesting alternative to reduce the impact of microbial spoilage on sliced cooked ham. © 2023 Society of Industrial Chemistry.

Sulphides from garlic essential oil dose-dependently change the distribution of glycerophospholipids and induce N6-tuberculosinyladenosine formation in mycobacterial cells

The antimicrobial properties of garlic are widely known, and numerous studies confirmed its ability to inhibit the growth of Mycobacterium tuberculosis. In this work, we explored the molecular mechanism of action of sulphides present in garlic essential oil against mycobacteria. The targeted transcriptomics and untargeted LC–MS metabolomics were applied to study dose- and time-dependent metabolic changes in bacterial cells under the influence of stressing agent. Expression profiles of genes coding stress-responsive sigma factors regulatory network and metabolic observations proved that sulphides from garlic essential oil are an efficient and specific agent affecting glycerophospholipids levels and their distribution within the cell envelope. Additionally, sulphides induced the Dimroth rearrangement of 1-Tuberculosinyladenosine to N6-tuberculosinyladenosine in mycobacterial cells as a possible neutralization mechanism protecting the cell from a basic nucleophilic environment. Sulphides affected cell envelope lipids and formation of N6-tuberculosinyladenosine in M. tuberculosis.

Physicochemical characterization, antioxidant and antifungal activities of essential oils of Urginea maritima and Allium sativum

Abstract This study aims to contribute to the valorizing of aromatic and medicinal plants of Morocco by studying the antioxidant and antifungal properties of the essential oils (EOs) of Allium sativum (AS) (garlic) and Urginea maritima (Syn. Drimia maritima) tested in vitro. After the hydrodistillation of EOs, we determined their physicochemical indices according to an international standard (Moroccan ISO standard), and the chemical composition of the oils was studied by gas chromatography coupled with mass spectrometry (GC-MS). Then, the antioxidant properties were determined using the free radical scavenging capacity of 2,2-diphenyl-1-picrylhydrazyl and the ferric-reducing antioxidant power of these oils. The evaluation of the antifungal activity was carried out by the direct contact method against two fungal strains, Fusarium oxysporum and Fusarium equiseti. GC-MS analysis revealed a significant percentage of terpenes in U. maritima EO, with terpinolene (21.82%) and estragole (6.24%) as the main compounds. Biological tests have demonstrated the remarkable antioxidant activities of this EO compared with AS EO, with an IC50 of 1.03 mg/mL and an RP0.5 of 1.60 mg/mL. On the other hand, while A. sativum (garlic) EO showed exceptional antifungal activities, with 100% inhibition and MICs of 0.5 µL/mL for F. equiseti and 2.5 µL/mL for F. oxysporum. Also, regardless of its antioxidant properties being moderate, this EO stands out for its efficacy against fungal infections of the Fusarium genus. In summary, the results suggest that U. maritima EO could be used as a natural source of antioxidants, while garlic EO could be considered a potentially effective treatment against fungal infections caused by Fusarium fungi.

Fabrication and release property of self-assembled garlic essential oil-amylose inclusion complex by pre-gelatinization coupling with high-speed shear

Our aim was to investigate the preparation of self-assembled garlic essential oil-amylose inclusion complexes (SGAs) using garlic essential oil (GEO) and corn starch (CS), and evaluated their release properties. SGAs were fabricated by pre-gelatinization coupling with high-speed shear at different GEO-CS mass ratios. When the mass ratio of GEO to pre-gelatinized corn starch was set at 15 % (SGA-15 %), with a fixed shear rate of 9000 rpm and a shear time of 30 min, the allicin content was 0.573 ± 0.023 mg/g. X-ray diffraction (XRD) results revealed a starch V-type crystalline structure in SGAs with peaks at 13.0°, 18.0°, and 20.0° (2θ). Fourier Transform Infrared (FTIR) spectra of SGAs displayed a shift in the characteristic peak of diallyl trisulfide from 987.51 cm−1 to 991.45 cm−1. Scanning electron microscope (SEM) images revealed that SGAs exhibited lamellar structures covered with small granules. SGAs exhibited higher residual mass (approximately 12 %) than other samples. The resistant starch content of SGAs increased from 10.1 % to 18.4 % as GEO contents varied from 5 % to 15 %. In vitro digestion tests showed that about 53.21 % of allicin remained in SGA-15 % after 8 h. Therefore, this dual treatment can be a new method for fabricating controlled-release inclusion complexes of guest molecules.

A novel aspect on different preservation methods for tomato paste by examining the protective effect of herb and spice oils.

In this study, the effect of essential oils of herbs and spices on the protection of homemade tomato paste without additives was investigated. Garlic oil was used as plant essential oil and thyme oil was used as spice essential oil. Samples were kept without adding essential oils at the specified holding times in determined light and dark ambient conditions. After the test period of the prepared setups was completed, the amount of mold formed in the tomato paste was observed, and the optimum samples were determined as K4A4, K4K7, K4K13, K6K10, S6K4, S6K7, S6K10, S6A13 by weighing again and creating a %-time graph by mass. It was decided that thyme essential oil had a more protective effect in foods than garlic essential oil, with the analysis methods performed on optimum samples (Physical analysis, microbiological analysis, FT-IR, and TG DTA).

Diallyl Trisulfide Causes Male Infertility with Oligoasthenoteratospermia in Sitotroga cerealella through the Ubiquitin-Proteasome Pathway.

Essential oils extracted from plant sources along with their biologically active components may have negative effects on insects. Diallyl trisulfide (DAT) is an active component of garlic essential oil, and it exhibits multi-targeted activity against many organisms. Previously we reported that DAT induces male infertility and leads to apyrene and eupyrene sperm dysfunction in Sitotroga cerealella. In this study, we conducted an analysis of testis-specific RNA-Seq data and identified 449 downregulated genes and 60 upregulated genes in the DAT group compared to the control group. The downregulated genes were significantly enriched in the ubiquitin-proteasome pathway. Furthermore, DAT caused a significant reduction in mRNA expression of proteasome regulatory subunit particles required for ATP-dependent degradation of ubiquitinated proteins as well as decreased the expression profile of proteasome core particles, including β1, β2, and β5. Sperm physiological analysis showed that DAT decreased the chymotrypsin-like activity of the 20S proteasome and formed aggresomes in spermatozoa. Overall, our findings suggest that DAT impairs the testis proteasome, ultimately causing male infertility characterized by oligoasthenoteratospermia due to disruption in sperm proteasome assembly in S. cerealella.

ANTIFUNGAL ACTIVITY AND SYNERGISM OF Allium sativum, Zingiber officinale, AND Citrus limon peel ESSENTIAL OILS AGAINST Tinea capitis

The burden of Tinea infections, common dermatologic fungal diseases remains high globally, especially in Africa. This is sustained mainly by the emergence of resistance to current antifungal drugs in use. Alternative treatment and preventive measures need to be sought to eradicate these infections. Antifungal activity and synergism of garlic in combination with ginger and lemon peel essential oils against Tinea capitis is assessed using inhibition assays. Ginger, lemons, and garlic samples were randomly collected from the Nakuru town market in Nakuru County, Kenya. They were identified by a botanist and then extracted and, analyzed. Antifungal activity against Tinea capitis and synergism among extracts were assessed by the disk diffusion method and minimum inhibitory concentration (MIC), respectively. The average essential oil yield for ginger, lemon, and garlic was 22%, 20%, and 16%, respectively. Ginger, lemon, and garlic essential oils depicted a comparable antifungal activity against Tinea capitis isolates (P value &gt; 0.05). The combined essential oils formulation of ginger, lemon, and garlic showed the highest activity compared to single extracts (P &gt; 0.05). The MIC for the combined essential oils was 6.25 mg/ml. A combination of the three extracts acts in synergy against the Tinea isolates. The rate of inhibition was directly proportional to the concentration of the extracts. Ginger, lemon, and garlic are sources of molecules that act in synergy in controlling Tinea spp infections. They can be leveraged to support the Ministry of Health in achieving its universal healthcare goal.