Aqueous Garlic Extract Research Articles

Emulsification Properties of Garlic Aqueous Extract: Effect of Heat Treatment and pH Modification.

Despite the broad research available in the literature dealing with garlic health benefits, little information is found regarding the functional properties of garlic components. The aim of this study was to evaluate the emulsification properties of garlic water-soluble compounds (GWSC), encompassing proteins, saponins, and carbohydrates, after heat treatment (10 min at 95 °C) or pH adjustments (2.5, 3.5, and 7.8). After the various treatments, the extracts were used as such or filtrated (0.45 µm), and 10% soybean oil-in-water emulsions were prepared using low (0.48%) or high (6.55% wt/wt) extract concentrations. Results showed that whereas at low GWSC concentrations, both heating and acidifying resulted in the formation of bigger oil droplet sizes (i.e., from d32 = 0.36 µm using unmodified extract to d32 = 7-22 µm at pH 2.5 with or without extract filtration), the effects were opposite at the highest GWSC concentration. In the latter, heat treatment clearly reduced the droplet size as observed from the micrographs as well as the degree of creaming, though the occurrence of depletion and/or bridging flocculation was still strong. The acidification of the extract at this high GWSC concentration significantly reduced the droplet size, as observed from the micrographs; however, a strong flocculation was observed. Removal of protein aggregates, and possibly also saponin micelles, from the extract resulted in an obvious increase in emulsion droplet size. This research brings valuable insights on this study and utilisation of novel natural food emulsifiers from plant sources.

Phytochemistry, Invitro Anti-Trypanosomal Efficacy and Acute Toxicity of ‎Allium sativum Linn. Bulb Aqueous Extract

Allium sativum Linn. is a bulbos perennial plant of the Family Liliaceae has been reported as having potential medicinal properties. This study was on the phytochemistry, in vitro anti-trypanosomal efficacy and acute toxicity of Allium sativum Linn bulb aqueous extract using standard procedures. Qualitative phytochemistry indicated high scores (+++) for reducing sugars, moderate scores (++) for alkaloids, steriods and carbohydrates with low scores (+) for tannins, flavonoids and saponins. The median lethal dose of the aqueous extract in albino rats was determined to be 2400 mg/kg body weight following intraperitoneal administration. The albino rats exhibited weakness, lordosis, starry hair coat, awkward posture, loss of apetite. All the albino rats died within 24 hours. There was a significant (p<0.05) reduction in mean parasites count (x106) on exposure in vitro to all the graded extract concentrations compared with the normal, and parasite count also decreasedwith time post-inoculation. Similarly, a positive correlation existed between extract concentrations and inhibition rates of the parasites post-inoculation. In conclusion, the aqueous extract of Allium sativum has in vitro anti-trypanosomal activity.

Biochemical and cellular (liver and kidney) restorative properties of garlic (Allium sativum) aqueous extract in cow brain-induced hypercholesterolemic model Swiss albino mice

Introduction and aim. Garlic is one of the most popular traditional medicinal herbs which has a number of desirable health benefits. The study was designed to depict the improvement of serum biochemical parameters as well as the histomorphological recovery potential of garlic aqueous extract in hypercholesterolemic mice. Material and methods. A total of thirty Swiss albino mice weighing 24±5g and aged 5 weeks were randomly divided into three groups. Group A: supplied standard mice pellet and water; Group B: standard mice pellet + hypercholesterolemic diet (cow brain: 2 g/kg b.w.t.); and Group C: standard mice pellet + hypercholesterolemic diet (cow brain: 2 g/kg b.w.t.) + garlic extract (25 ml/kg b.w.t.). After four weeks of experimental tenure, samples (blood, liver, and kidney) were collected from each group of mice for serum biochemical analysis and histomorphological study. Results. Compared with hypercholesterolemic mice, total cholesterol (TC), triglyceride (TG) concentration, and low-density lipoprotein (LDL) levels significantly decreased respectively by 7%, 20% and 48% along with high-density lipoprotein (HDL) levels significantly increased by 47% in garlic extract supplemented group. Based on the histological evaluation in the liver sample of group C, both portal and central veins were normal, and fat droplets were not found in the hepatocytes which were found in the liver of group B. On the other hand, unchanged renal cortex, glomerulus, Bowman’s space, and kidney tubules were seen in group C. Conclusion. Therefore, the above findings of the present research would assist to provide affirmation about the cholesterol-decreasing and cellular restoration potentiality of garlic aqueous extract.

Effect of different concentrations of garlic extract on dental plaque isolates obtained from patients attending Federal Medical Center Makurdi

Instead of the traditional microbiological pathogens, the microbes that make up the mouth's resident micro-flora are what cause oral diseases like dental caries/plaque and periodontal disorders. The aim of this study was determine the effect of different concentrations of garlic extract on dental plaque isolates obtained from patients attending Federal Medical Center Makurdi. Swap samples were collected from teeth of patients and were transported to the laboratory, analysis was done on different culture media and identification of isolates was done using the Bergy’s manual of determinative bacteriology, biochemical characterization was carried following standard microbiological methods. The antibacterial activity was done using agar well diffusion method in which the garlic extract was introduced in concentrate. Concentrations of 500 mg/mL, 250 mg/mL, 125 mg/mL, 65.3 mg/mL and 35.5mg/mL were prepared and used for the susceptibility. The Isolates from results were Streptococcus oralis, Streptococcus mutans, Enterococcus feacalis and Staphylococcus aureus. The effect observed was concentration dependent as all the graphs obtained shows a linear series, the lowest inhibitory concentration shows that 250mg/ml was the lowest concentration that inhibits each of the isolates in ethanolic garlic extract while 500 mg/mL was the minimum inhibitory concentration for the aqueous garlic extract. Oral hygiene was recommended in order to help reduce the accumulation of dental plaque which usually harbours primary microbes that colonize the teeth surfaces resulting to caries and periodontal diseases. The use of mouth wash containing garlic and or toothpaste incorporated with alicin was recommended as this can help reduce the accumulation of dental plaque biofilm thereby minimising the incidence of dental caries and periodontal diseases.

Formulations and Evaluations of Herbal Anti-Acne Gel from Coriander and Garlic

Abstract: Acne is an inflammatory condition of the skin’s sebaceous follicles. The goal of the current study was to create and assess a coriander and garlic aqueous extract topical anti-acne gel. The antibacterial activity of corianders and garlic aqueous extract against Propionibacterium acnes and Staphylococcus epidermidis was examined. Agar dilution method was used to calculate concentration. The physical characteristics, Spreadibility, extrudability, ph, viscosity of the topical formulations was designed and tested. There are many pharmacological effects of garlic, including antibacterial, anticancer, and antiinflammatory effects. Fresh pearl garlic or garlic with a single clove was utilised as the raw material in this study to make the garlic extracts. This project’s goal is to get the garlic ready. Extracts made with non-chemical methods. The non-chemical extraction, the garlic extract was made by combining honey and garlic in a 1:1 ratio (w/v). The results showed that formulation F1 had the highest drug content (94%), highest level of stability, and zone of greatest efficacy, inhibition among all formulations.

Green synthesis of Fe3O4 nanoparticles using Alliaceae waste (Allium sativum) for a sustainable landscape enhancement using support vector regression

The synthesis of metal nanoparticles using green chemistry methods has gained significant attention in the field of landscape enhancement. Researchers have paid close attention to the development of very effective green chemistry approaches for the production of metal nanoparticles (NPs). The primary goal is to create an environmentally sustainable technique for generating NPs. At the nanoscale, ferro- and ferrimagnetic minerals such as magnetite exhibit superparamagnetic properties (Fe3O4). Magnetic nanoparticles (NPs) have received increased interest in nanoscience and nanotechnology due to their physiochemical properties, small particle size (1–100 nm), and low toxicity. Biological resources such as bacteria, algae, fungus, and plants have been used to manufacture affordable, energy-efficient, non-toxic, and ecologically acceptable metallic NPs. Despite the growing demand for Fe3O4 nanoparticles in a variety of applications, typical chemical production processes can produce hazardous byproducts and trash, resulting in significant environmental implications. The purpose of this study is to look at the ability of Allium sativum, a member of the Alliaceae family recognized for its culinary and medicinal benefits, to synthesize Fe3O4 NPs. Extracts of Allium sativum seeds and cloves include reducing sugars like glucose, which may be used as decreasing factors in the production of Fe3O4 NPs to reduce the requirement for hazardous chemicals and increase sustainability. The analytic procedures were carried out utilizing machine learning as support vector regression (SVR). Furthermore, because Allium sativum is widely accessible and biocompatible, it is a safe and cost-effective material for the manufacture of Fe3O4 NPs. Using the regression indices metrics of root mean square error (RMSE) and coefficient of determination (R2), the X-ray diffraction (XRD) study revealed the lighter, smoother spherical forms of NPs in the presence of aqueous garlic extract and 70.223 nm in its absence. The antifungal activity of Fe3O4 NPs against Candida albicans was investigated using a disc diffusion technique but exhibited no impact at doses of 200, 400, and 600 ppm. This characterization of the nanoparticles helps in understanding their physical properties and provides insights into their potential applications in landscape enhancement.

The effect of garlic extract and volatile oil on growth of Trichophyton mentagrophytes

This study was conducted in the College of Medicine- laboratory of microbiology to assess the effect of crude garlic extract and essential volatile oil on growth of Trichophyton mentagrophytes. Antidermatophytic activity of different concentrations of volatile oil, aqueous extract of garlic were investigated in vitro against T. mentagrophytes by using agar dilution technique. The results showed that minimal inhibitory concentration (MIC) of aqueous garlic extract (crude) was 0.5 mg/ ml, while the MIC of essential oil was 0.25 mg/ml

In Vitro Embryo Production of Doe Rabbits Administrated with Aqueous Garlic Extract

In Vitro Embryo Production of Doe Rabbits Administrated with Aqueous Garlic Extract

Metabolite Formation by Fungal Pathogens of Potatoes (Solanum tuberosum L.) in the Presence of Bioprotective Agents

The potato is a crop of global importance for the food industry. This is why effective protection against pathogens is so important. Fungi as potato pathogens are responsible for plant diseases and a significant reduction in yields, as well as for the formation of mycotoxins. This study focuses on the effect of three natural biocides, yeast Metschnikowia pulcherrima, lactic acid bacteria Lactiplantibacillus plantarum, and aqueous garlic extract, on the improvement of the physiology of planted potato tubers and the reduction in mycotoxin formation. The secondary metabolites produced by the fungal pathogens of genera Fusarium, Alternaria, Colletotrichum, Rhizoctonia, and Phoma in the presence of these biocontrol agents were compared to profiles obtained from contaminated potatoes. Analysis of liquid chromatography coupled with tandem mass spectrometry data showed the presence of 68 secondary metabolites, including the mycotoxins: alternariol, alternariol methyl ether, altertoxin-I, aurofusarin, beauvericin, diacetoxyscirpenol, enniatin B, and sterigmatocystin. The studies showed that the applied biocontrol agents had a positive effect on the physiological parameters of potatoes (including root growth, stem growth, gas exchange, and chlorophyll content index) and on the reduction in the production of mycotoxins and other secondary metabolites by Fusarium, Alternaria, and Phoma.

In vitro and in silico investigation of garlic’s (Allium sativum) bioactivity against 15-lipoxygenase mediated inflammopathies

Introduction: Garlic (Allium sativum) is widely used as a flavor-enhancing dietary ingredient and exhibits a wide spectrum of pharmacological effects. This study aimed to investigate the therapeutic effects of aqueous garlic extract to explore the bioactivity against 15-lipoxygenase (15-LOX) mediated inflammopathies. Methods: In this study, the antioxidant (DPPH free radical scavenging assay and reducing power assay), anti-inflammatory (hypotonicity-induced hemolysis assay and 15-LOX inhibition assay) and anticoagulation (serine protease inhibition assay and prothrombin time assay) effects of the aqueous garlic extract were investigated. Furthermore, in silico molecular docking and dynamic simulation analysis of reported small compounds of garlic against 15-LOX1 and 15-LOX2 were performed to figure out the most efficient phytochemical ligands and validate the anti-inflammatory potential. Results: The DPPH scavenging effect and the reducing power of the extract were found with the IC50 of 213.87 ± 1.49 μgmL-1 and EC50 of 124.78 ± 3.39 μgmL-1, respectively. In the hypotonicity-induced hemolysis and 15-LOX inhibition assay, the IC50 values were observed as 147.59 ± 2.98 μgmL-1 and 250.05 ± 8.48 μgmL-1, respectively. The extract inhibited serine protease activity with an IC50 of 301.33 ± 1.31 μgmL-1 and prevented blood coagulation for 10.05 ± 0.35 minutes in prothrombin time assay. The in silico study identified Rhamnetin as a potential 15-LOX1 and 15-LOX2 inhibitor, and it exhibited a stable interaction with the targets throughout the 100 ns dynamic simulation. Conclusion: The findings of this study provide molecular insights into garlic’s medicinal properties as well as its bioactive compounds, which can be potential therapeutic interventions for 15-LOX mediated inflammations.

Investigation of taurine and aqueous garlic extract diet supplementation effect on the healing of rat osteoporotic fractures.

We have evaluated the effects of taurine and aqueous garlic extract (AGE) as a dietary supplement on osteoporotic fracture (OPF) healing in the ovariectomized rat femur fracture model. In this experimental animal study,twenty-four osteoporosis-remodeled female Wistar albino rats were randomly divided into 3 groups (n: 8) according to their supplemented diet; control, taurine, and AGE groups. Unilateral femur middiaphysis mini-open osteotomy was stabilized with Kirschner wires. Six weeks after osteotomy, the rats were sacrificed before the femurs were harvested and OPF healing was evaluated with biochemical, histologic, microcomputed-tomography, and scintigraphic methods. As an indicator of the antiosteoporotic effect, the calcium levels of the taurine group were significantly lower than the AGE and control groups in biochemical analyzes (p < 0.01). In histological studies, the new bone diameter and new bone volume values of the taurine group were significantly higher than the control group (p = 0.002 and p = 0.032, respectively), while higher trabecular-compact callus was observed in the taurine and AGE groups, respectively, compared to the control group. In morphological analyses, taurine and AGE groups had significantly higher bone volume/tissue volume, trabecular number, bone surface density, and lower trabecular separation than the control group (p < 0.05). The scintigraphic imaging showed a significant increase in osteoblastic activity of the taurine group compared to the control group (p = 0.005). Taurine and AGE have positive anabolic effects, respectively, on the healing of OPFs, demonstrated by biochemical, histological, morphological, and scintigraphic methods.

Effects of aqueous garlic (Allium sativum) extract against di-(2-ethylhexyl) phthalate induced cytotoxicity in peripheral blood and liver of adult female mice

Effects of aqueous garlic (Allium sativum) extract against di-(2-ethylhexyl) phthalate induced cytotoxicity in peripheral blood and liver of adult female mice

Comparison of In Vitro Anticoagulant Activity of Raw, Boiled, and Honey Fermented Allium sativum (GARLIC)

Garlic (Allium sativum) is one of the medically beneficial spices consumed by Sri Lankan people in different ways. The study aimed to determine the bioactive compounds and in vitro anticoagulant activity of aqueous and methanolic extract of raw, boiled, and honey fermented preparations of garlic. Different concentrations of aqueous and methanolic extract of raw, boiled, and honey fermented garlic were prepared by grinding different weights of garlic (2.5×10-3 kg for 500 mgmL-1, 1.25×10-3 kg for 250 mgmL-1, 0.25×10-3 kg for 50 mgmL-1 and 0.05×10-3 kg for 10 mgmL-1). For aqueous extract, the crude extract had been collected whereas for methanolic extract preparation, maceration had been done. In vitro anticoagulant activity was analysed using prothrombin time (PT) of pooled plasma diluted with different concentrations of garlic extract. Bioactive compounds in garlic extracts were analysed by Gas Chromatography-Mass Spectrometry. Methanolic extract of all 3 preparations and aqueous extract of honey fermented garlic had significantly prolonged PT at all concentrations compared to the control (p&lt;0.05). Aqueous extract of raw and boiled garlic showed significant prolongation in PT only at high concentrations compared to the control (p=0.008). Prolongation in PT was increased with increasing concentration of garlic extract. Honey fermented garlic had the highest prolongation in PT compared to the other two preparations. Moreover, methanolic garlic extract exhibited the a higher prolongation in PT compared to aqueous garlic extract. The content of Dodecanoic acid methyl ester and Methyl tetradecanoate in boiled garlic extract was much higher than in raw garlic extract. Diallyl disulphide, Methyl thiourea and S-Methyl methanethiosulfinate were only found in aqueous raw garlic extract. Beta sitosterol was only detected in methanolic raw garlic extract. All three consumption methods of garlic have an inhibitory effect on blood coagulation. Honey fermented garlic is the most effective preparation for anticoagulant activity.

Evaluation of Seed Germination of Barley Grown after Priming with Leaf Extract of Moringa and Aqueous Garlic Extract

A pot experiment was designed to determine the effect of seed priming on the germination of barley seeds in the winter season. Seed priming was done by using the leaf extract of Moringa at different concentrations (100%, 50% and 10%v/v) for 24 hours, and aqueous extract of ginger and tap water for different intervals of time (6, 12 and 24 hours). The experiment was continued for ten days with nine seed priming treatments. The results showed that maximum seeds germinated on day nine in all treatments. Whereas the highest germination of seeds was observed when seeds were primed with an aqueous extract of garlic for 24 hours and when seeds were primed with 100% concentration of Moringa leaf extract for 24 hours. The lowest seed germination was observed when seeds were primed with an aqueous extract of garlic for 6 hours. These results showed that Moringa leaf extract and aqueous garlic extract can be used to prime barley seeds to improve germination.

AMELIORATIVE EFFECT OF GARLIC ON CHOLESTEROL INDUCED MORPHOLOGICAL CHANGES ON THE CARDIOVASCULAR WALL OF ADULT WISTAR RATS

This study is designed to investigate the effect of cholesterol and cholesterol in thepresence of aqueous extract of garlic on the cardiovascular wall of adult Wistar rats.A total of twenty Wistar rats (both sex) weighing 200-275 grams were used in thestudy. The rats were randomly assigned into four groups of five rats per group.Group A served as the control while Group B, C and D served as the treatmentgroups. Group A received no treatment, Group B was fed a high cholesterol diet,Group C received garlic treatment and Group D was fed a high cholesterol diet andalso received garlic treatment. The Aqueous garlic extract was administered at adose of 250mg /kg / body weight via orogastric tube for a period of 30 days (4weeks). The rats were sacrificed by cervical dislocation method on the 31st day of theexperiment. Blood sample was collected by cardiac puncture for cholesterol analysis.The heart and blood vessel was carefully harvested and fixed in 10% formal salinefor histological studies using haematoxylin and eosin procedures. The Findingsindicated that high cholesterol fed rats when compare with the control showed thatthere was a significant increase in cholesterol level (P &gt; 0.05) and also there wasno significant difference in the cholesterol level (P &gt; 0.05) in garlic treated rats fedwith high cholesterol diet. The rats fed a high cholesterol diet showed a significantdamage to the intima of blood vessel of the heart and other blood vessels as well asconsequent myocardial injury. However with injection of garlic there was a protectiveresponse by reduction in blood cholesterol and activation of the local immunesystem. This led to minimal injury to both the heart and blood vessels. It isrecommended that garlic may be beneficial to reduce damage of cholesterol to thecardiovascular and induce local protective immune response.

Investigation of bioactive compounds and antimicrobial properties of aqueous garlic extracts on important food-borne zoonotic bacteria for food applications

Investigation of bioactive compounds and antimicrobial properties of aqueous garlic extracts on important food-borne zoonotic bacteria for food applications

Garlic and allopurinol alleviate the apoptotic pathway in rats' brain following exposure to fipronil insecticide.

Fipronil can cause oxidative tissue damage and apoptosis. Our goal is to evaluate the antiapoptotic impact of garlic or allopurinol against fipronil neurotoxicity. Thirty-six mature male albino rats were separated into control, garlic aqueous extract (500 mg/kg), allopurinol (150 mg/L in their drinking water), fipronil (13.277 mg/kg), garlic+fipronil, and allopurinol+fipronil. Our results revealed that fipronil induced a significant increase in brain malondialdehyde, protein carbonyl levels as well as enzymatic antioxidant activities (superoxide dismutase, catalase, glutathione peroxidase, and xanthine oxidase), but glutathione-S-transferase recorded a significant decrease as compared to the control. In addition, fipronil significantly up-regulated the brain pro-apoptotic (Bax) and caspase -3 mRNA gene expression and induced DNA fragmentation but caused down-regulation in anti-apoptotic (Bcl-2) mRNA genes expression. Interestingly, co-administration with garlic or allopurinol improved the lipid peroxidation, antioxidant disturbance, and apoptosis induced by fipronil in the brain tissues. In conclusion, garlic or allopurinol reduced fipronil-induced apoptosis and reduced oxidative tissue damage, most likely through enhancing the tissue antioxidant defense system.

In-vitro Antibacterial Activity of Allium sativum on Common Food Poisoning Bacteria

Background: The use of herbal medicines for food poisoning in inadequate resource countries has greatly increased with the limited establishment of antibacterial activity. This study aimed at establishing the antibacterial activity of the aqueous Allium sativum extract against Staphylococcus aureus, Escherichia coli and Salmonella typhimurium.&#x0D; Methods: Allium sativum L. (Amaryllidaceae) was purchased from a farmer in Kasese District and was authenticated. The material was then extracted using distilled water, filtered and lyophilized. Evaluation of the phytochemicals in the extract was done using standard procedures. High-Performance Liquid Chromatography (HPLC) fingerprint of the extract was conducted to monitor the reproducibility of the extract. The antibacterial activity of the aqueous garlic extract against the organisms above was evaluated and expressed as Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC).&#x0D; Results: Phytochemical screening of the garlic extract showed the presence of amino acids, alkaloids, glycosides and saponins, flavonoids, steroids and terpenoids. HPLC fingerprints showed 39 and 98 peaks at 220nm and 254nm wavelengths respectively and the presence of various phytochemical compounds in A. sativum. The extract showed antibacterial activity against S. aureus, E. coli and S. typhi.&#x0D; Conclusion: The aqueous garlic extract exhibited antibacterial activity against tested common food poisoning bacteria, with a high potency against gram-positive bacteria.

Curative Potentials of Garlic (Allium sativum) Extract against Di-(2-Ethylhexyl) Phthalate Induced Reproductive Toxicity in Female Mice

The present study was intended to find out the curative potentials of Garlic (Allium sativum) against di-(2-Ethylhexyl) phthalate (DEHP) induced toxicity in the reproductive system of female mice. Forty female mice were divided into four groups (n=10) as the (a) control group was given normal feed and drinking water, (b) aqueous garlic extract treated group (500 mg/kg), (c) DEHP group received 500 mg/kg in corn oil, and (d) DEHP + aqueous garlic extract each at a dosage of 500 mg/kg body weight. All treatments were given daily through oral gavage for 28 days. After completion of the experiment, all the animals were dissected through cervical dislocation to get reproductive organs. Collected organs were weighed and processed through the conventional histology technique of staining with eosin and hematoxylin. This study indicated that DEHP exposure caused a significant decrease (P&lt;0.001) in body weight and weight of the complete female reproductive tract as compared to the control group, while the garlic co-administered group showed prominent improvement in body and organ weight when compared to only DEHP treatment. The adverse effect of DEHP on the histology of the ovary such as a decreased mean number of developing follicles, thin and irregular corona radiata, disruption of cumulus oophorus, and reduction in the size of the antral cavity of the tertiary follicle was observed. However, a significant recovery in the development of follicles was seen in DEHP plus garlic-treated group. DEHP plus garlic extract treatment showed protective effects on the uterus, such as a significant increase in the diameter of the uterus (P&lt;0.001), muscularis, mean number of endometrial glands (P&lt;0.001), and endometrial epithelial heights as compared to only DEHP exposed group. Hence, garlic extract showed significant ameliorative potential against DEHP-induced reproductive anomalies in female mice.

Green Ag/AgCl as an Effective Plasmonic Photocatalyst for Degradation and Mineralization of Methylthioninium Chloride

A green synthesis of Ag/AgCl with an exceptional SPR and photocatalysis property is greatly benefit to the environmental application especially pollutant removal. In this work, a novel green plasmonic photocatalysis of Ag/AgCl nanocatalyst using aqueous garlic extract (Allium Sativum L.) was successfully synthesized. The allicin and organosulfur compounds in the garlic can act as reducing agents in the green synthesis process. The nanocatalyst properties were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffractometer. The light-harvesting property was investigated by UV-vis absorption spectra which reveals its visible light absorption capability owing surface plasmon resonance behavior of Ag nanoparticles. The degradation and mineralization of methylthioninium chloride (MC) using this photocatalyst were evaluated under visible light and natural solar irradiation. Surface plasmon resonance of Ag nanoparticles and the presence of organosulfur from the garlic extract facilitated adsorption of MC onto the particle surface, promoting greater degradation. The photocatalytic reaction under visible light can be explained by the pseudo first-order pattern with the highest reaction rate of 0.5829 mg L−1 min−1 at pH 10. The photocatalytic activity of the Ag/AgCl under the natural sunlight reached 90% and 75% for MC and total organic carbon (TOC), respectively. The intermediate products detected during MC degradation under sunlight irradiation before final transformation to CO2, H2O, HNO3, and H2SO4 were also reported. The simplicity of Ag/AgCl green synthesis with the photocatalysis properties under visible light and sunlight can offer the convenience of applying these nanoparticles for pollutant removal in water treatment processes.