Garlic Allium Research Articles

Effect of vermicompost on yield, quality and antibacterial activity of garlic.

We studied the influence of humus and different norms of vermicompost on the yield, nutritional value, and antibacterial properties of common garlic (Allium sativum L.). The use of the vermicompost caused an increase in the garlic yield by 1.7-3.9 t ha-1 for cultivar "Prometei" compared to the control "Sofiiskyi" and by 2.2-5.2 t ha-1 in relation to the control of cultivar "Prometei". It was established that the calory content could increase up to 22.68 g 100 g1 of fresh weight with vermicompost manure 5 t ha-1, and the total content of sugar is increasing by 21.52-40.81% depending on the cultivar. The content of vitamins increased alongside with rate of vermicompost introduction. We also revealed the influence of variety factor on the accumulation of vitamins. The antibacterial effect of the garlic essential oils against Staphylococcus aureus, Escherichia coli and Bacillus subtilis was significant; the diameters of the inhibition zone were for S. aureus 21.35-27.10 mm, for E. coli - 16.97-26.46 mm, and for B. subtilis - 16.42-25.36 mm. The number of Mycobacterium smegmatis colonies decreased by 23.96-43.44%. This study has been proved that garlic juice had considerable antibacterial effect on the studied bacteria (S. aureus, E. coli and B. subtilis); therefore, it can replace chemical antibiotics that have undesirable effects on the body, like allergy and antimicrobial resistance. Our data shows that using of vermicompost was more effective than humus.

Does Allium sativum L. tolerate water deficit?

The objective of this work was to evaluate the effect on the physiological and biochemical aspects of garlic submitted to water deficit, knowing that the popular garlic Allium sativum L. is a culture known worldwide for its medicinal, culinary and ecological properties of agricultural pests. were constituted with water condition of 100; 75; 50 and 25% of pot capacity (CP). Three bulbs were planted per pot (5 L) containing Plantmax® substrate, irrigated every two days, according to pot capacity. The chlorophyll index was evaluated during the first and last week of greenhouse treatments. At 45 days after the treatments, the garlic plants were harvested to evaluate the physiological and biochemical parameters. Garlic plants showed to be sensitive to water deficit, resulting in lower leaf length and fresh biomass. Water stress induced the activity of the APX enzyme in the leaves and of the POD in the roots, as well as increase of proteins in the bulbilhos as biochemical mechanisms of adaptation.

IN VITRO EVALUATION OF ANTIBACTERIAL ACTIVITY OF GARLIC ALLIUM SATIVUM AGAINST POULTRY PATHOGENS AND EFFECT OF GARLIC SUPPLEMENTATION ON DUCKLING GROWTH PERFORMANCE

ABSTRACT-QMFS2019Poultry production provides source of protein and contributes an important income for Vietnamese farmers. Among the poultry in Vietnam, ducks account for 27.3% of head of poultry and even 55.7% in Mekong Delta region. Along with the development of rearing ducks, bacterial, viral and fungal diseases occurring in the two last decades induced bad effect for poultry producer. Escherichia coli, Salmonella enterica, Streptococcal or Pasteurella act as major pathogenic bacteria in duck. The aims of this study were to investigate the antibacterial activity of garlic Allium sativum against E. coli, Staphylococcus aureus, Salmonella Typhimurium and to evaluate the effect of garlic on growth performance of duck from 1-28 old-days. The results indicated that fresh garlic and dried garlic powder showed inhibitory effect against pathogenic tested strains from 2% and 4% w/v, respectively. The inhibition zones and the minimal inhibitory concentration (MIC) values of garlic extract ranged from 11.3-28.3 mm and 0.02-0.2 g/ml, respectively. After 28 days of diet with garlic supplemented, D3 (2% of fresh garlic in water) showed significantly different in weight gain (WG), feed conversion ratio (FCR), protein efficiency ratio (PER), average daily weight (ADW); whereas, D2 (2% of garlic powder in basal diet) only possessed a difference significant in feed consumption (FC) compared to the D1 (control without garlic supplementation). The obtained results demonstrated the potential of garlic application in poultry production.

Effect of low temperature conditioning on flavor precursors of ‘Coreano’ garlic (Allium sativum L.) “seed” cloves

ISHS XXX International Horticultural Congress IHC2018: II International Symposium on Root and Tuber Crops: Value Added Crops for the Next Generation Effect of low temperature conditioning on flavor precursors of ‘Coreano’ garlic (Allium sativum L.) "seed" cloves

Comparative effects of different concentrations of garlic (Allium sativum) and ginger (Zingiber Officinale) on growth performance, goblet cell histochemistry and gut microbiota of broilers

The present study investigates comparative effects of different concentrations of garlic (Allium sativum) and ginger (Zingiber Officinale) on growth performance and selected histo-microbial parameters of broilers. A total of 80 one day- old chicks were divided into five different groups with each group having 4 replicates in a trial of 42 days. The first group was kept as control whereas, the second, third, fourth and fifth groups were given garlic 0.25%, garlic 0.5%, ginger 0.25% and ginger 0.5% respectively. At the end of trial all birds were slaughtered for analysis. Results revealed that feed conversion ratio and live body weight were significantly (P < 0.05) improved by garlic 0.5% supplementation compared to other groups. Histo-microbiology revealed that lactic acid bacteria, yeast, acidic goblet cells, mixed goblet cells and total goblet cells increased significantly (P < 0.05) by the dietary supplementations under study. In was concluded that 0.5% garlic supplementation was a better alternate to antibiotic in broilers.

EFFECT OF SULFUR AND ORGANIC FERTILIZERS ON GROWTH AND YIELD OF GARLIC ALLIUM SATIVUM L.

A field experiment was carried out during agricultural season 2011/2012 to study the effect of sulfur addition with some organic fertilizers on plant growth and the yield of garlic Allium sativum L. which has been got as cloves from local markets. Experiment was conducted according to the randomized complete block designe withing factorial experiments by three replicates. Using Duncan test polynomial on possibility of 5% to compare averages of treatments, Eighteent sulfur addition was applied levels of zero and 1000 kg.ha-1 and poultry manures of 10 and 20 tons.ha-1. Some fertilizers and organic extracts such as Italpollin in levels 1 and 2 tons.ha-1. Afoiler organic extracts were applied on levels 2.5 and 5 ml.liter-1. The results showed that sulfur addition at a rate of 1000 kg.ha-1 caused a significant increase on chlorophyll leaves content and plants harvested, cloves number, mean clove weight, total heads yield and biological yield, poultry manure application at the level of 20 tons.ha-1 appeared also a significant increase as above, also intraction between sulfur and levels of added organic fertilizers increased the above studied properties, 1000 kg.ha-1 of sulfur with (20 tons.ha-1) of organic fertilized had a significant increase on studied properties accept mean weight of clove and

Screening of immuno-modulatory potential of different herbal plant extracts using striped catfish (Pangasianodon hypophthalmus) leukocyte-based in vitro tests

Many medicinal plants have been shown to possess biological effects, including immuno-modulatory activities on human and other mammals. However, studies about the potential mechanisms of plant extracts on the humoral and tissular immunities in fish have received less attention. This study aimed to screen the immunestimulating properties of 20 ethanol plant extracts on striped catfish Pangasianodon hypophthalmus leukocytes. The peripheral blood mononuclear cells (PBMCs) and head kidney leukocytes (HKLs) of striped catfish (50 ± 5 g per fish) were stimulated at 10 and 100 μg of each plant extract per mL of cell culture medium. Several humoral immune parameters (lysozyme, complement and total immunoglobulin) were examined at 24-h post stimulation (hps). Furthermore, the responses of four cytokine genes, namely il1β, ifrγ 2a and b, and mhc class II were assessed by quantitative real-time PCR at 6, 12, 24, and 48 hps. The results showed that lysozyme, complement as well as total immunoglobulin levels in both PBMCs and HKLs were regulated by some of the plant extracts tested in a concentration-dependent manner; some plant extracts induced the highest immune responses at the low dose (10 μg mL−1) while others were more efficient at high dose (100 μg mL−1). Among the extracts, five extracts including garlic Allium sativum L. (As), neem Azadirachta indica A. Juss (Ai), asthma-plant Euphorbia hirta L. (Eh), bhumi amla Phyllanthus amarus Schum. et Thonn (Pa), and ginger Zingiber officinale Rosc (Zo) induced significant changes in the expression of pro-inflammatory cytokine (il1β), antiviral cytokines (ifrγ 2a and b) and adaptive immune cytokine (mhc class II) in striped catfish cells. Pa always modulated the strongest expression of the four cytokines in PBMCs and HKLs over the whole experimental period (p < 0.05), whereas Zo did not stimulate the mhc class II expression in striped catfish leukocytes throughout experimental periods. These in vitro results demonstrated that some plant extracts could differently modulate great potential immune response in fish, supporting their applications in further in vivo experiments.

Mollifying actions of garlic (Allium sativum) against Chloropyrifos induced toxicity by using rabbit (Oryctolagus cuniculus) as a model subject

Mollifying actions of garlic (Allium sativum) against Chloropyrifos induced toxicity by using rabbit (Oryctolagus cuniculus) as a model subject

ANTIOXIDANT ACTIVITY IN COMBINATION EXTRACT OF Acorus calamus L. (DLINGU) AND Allium sativum (GARLIC)

One of the efforts to optimize the utilization of natural materials that is used them as medicinal plants, including Dlingu (Acorus calamus L.) and Garlic (Allium sativum) which have the potential as antioxidants. This study aims to determine the effectiveness of a combination extract of Acorus calamus L. (Dlingu) and Allium sativum (Garlic) on antioxidant activity. This research is an experimental research which is the test of antioxidant content and antioxidant activity test with 5 series of concentrations and 4 times of repetition. The research materials, namely dlingu stem and single clove garlic tubers were extracted, phytochemical screening was carried out and then antiradical free IC50 was determined. Based on the test results of the chemical extract of dligu and single clove garlic, the highest content was alkaloid 5.16%, tannin 4.05%, saponin 3.01%, and flavonoid 2.18%. The test results of the chemical antioxidant activity of extracts dligu and single clove garlic contain IC50 values of 17.062 ppm, including a very strong class of antioxidants.Keywords: antioxidant, Dlingu, Single Clove (Male) Garlic.

Molecular identification, incidence and phylogenetic analysis of seven viruses infecting garlic in Ethiopia

Little information exists on the type and incidence of viruses infecting garlic (Allium sativum L) in Ethiopia. Attempts were made to identify the viruses using molecular techniques from 95 composite leaf samples collected from 44 farmers’ fields and 51 germplasm accessions. Reverse transcription (RT-) PCR using genus and/or virus specific primers was used to amplify partial genome sequences of potyviruses, allexiviruses, carlaviruses and a tospovirus followed by sequencing of PCR products. Results indicated that ~73.7% of the samples are infected with at least one virus. Onion yellow dwarf virus (OYDV, genus Potyvirus, family Potyviridae) is the most common virus detected followed by Garlic virus C (genus Allexivirus) and Shallot latent virus (SLV, genus Carlavirus). Other viruses detected at lower frequency include Garlic virus X and Garlic virus D (genus Allexivirus), Leek yellow stripe virus (genus Potyvirus) and Iris yellow spot virus (IYSV, genus Tospovirus). Mixed infection of two or more viruses was detected in 65.7% of the samples. Phylogenetic analysis suggested that the different viruses may have been introduced to Ethiopia from Europe or Asia. This is the first report of Garlic virus X, Garlic virus D, IYSV and SLV in garlic in Ethiopia. The high incidence of OYDV and IYSV which cause severe yield loss alone or in mixed infection with allexiviruses and carlaviruses is a cause of concern to growers.

Levels of Essential and Non-essential Metals in Garlic (Allium sativum L.) Samples Collected from Different Locations of East Gojjam Zone, Ethiopia

The concentrations of essential metals (Na, K, Ca, Mg, Fe, Zn, Mn, Cu, Co and Ni) and non-essential metals (Cd and Pb) in garlic samples were determined using flame photometer for Na and K, and flame atomic absorption spectrometry (FAAS) for the rest of the metals. The samples were collected from four agricultural areas (Debre Markos, Dejen, Bichena and Debre Werk), East Gojjam Zone, Ethiopia. Wet digestion method using a mixture of 4 ml HNO 3 and 1 ml HClO 4 was used for digestion of the samples. The results obtained revealed that the concentrations of metals in the garlic samples in mg/kg dry weight were in the range of: Na (244–306), K (6514–8592), Ca (3142–4004), Mg (798–1322), Fe (136–172), Zn (57.6–83.6), Mn (27.2–27.5), Cu (8.15–13.4), Co (11.4–28.3), Ni (14.1–19.6), Cd (0.10–0.14) and Pb (0.68–1.43), respectively. In general, the levels of metals in Allium sativum collected from all sampling site was found to be decreased in the order of: K > Ca > Mg > Na > Fe > Zn > Mn > Co > Ni > Cu > Pb > Cd. The levels of metals determined in the analyzed garlic samples were found below the WHO maximum permissible limit; hence they are safe for human consumption and can be considered as good sources of essential nutrients

Screening for antifungal activity of garlic (Allium sativum) powder against mycelia growth of three post-harvest pathogens

Screening for antifungal activity of garlic powder against mycelia growth of three post-harvest pathogens (Aspergillus, Rhizopus and Mucor species) was investigated in this study. Five grams of malt extract agar (MEA) were poured into a conical flask, 100 ml of water and different weight of garlic powder (1, 3, 5 and 7 g) were separately added, stirred and later sterilized while MEA medium with no garlic added (0 g) served as control. The mycelia of each post-harvest pathogen was cut with 6mm cork borer and placed on the solidified medium in the Petri dish and incubated at 28±2oC for 72 hours. Phytochemical screening of the garlic powder was also investigated. Results from this study showed that the different weights of the garlic powder apart from the control (0 g garlic) significantly inhibited the mycelia growth of the three post-harvest pathogens tested in the study and the order of antifungal activity of the garlic powder against mycelia growth of Aspergillus, Rhizopus and Mucor species was 7 g > 5 g > 3 g > 1 g > 0 g, 5 g > 7 g >1 g > 3 g > 0 g and 7 g > 5 g > 3 g > 1 g > 0 g respectively. The antifungal activity of the garlic powder may be related to the presence of active antimicrobial agents including alkaloids, saponins, tannins, flavonoids and cardiac glycosides that were detected in the powder. DOI: http://dx.doi.org/10.5281/zenodo.2635824

Reservoir Conservation in a Micro-Watershed in Tigray, Ethiopian Highlands

Soil erosion in Ethiopian highlands has caused land deterioration due to moving nutrient-rich top soil to downstream reservoirs while leaving reservoirs dysfunctional due to sedimentation. Micro-watershed management by removing reservoir sediments and using them for reclaiming farmland, while using reservoir water for irrigation, can be a potential solution to simultaneously address soil and water constraints and food security challenges. Still, there is knowledge gap before such a solution can be practically applied. The objective of this paper is to present potential solutions for the reservoir sedimentation problem and specifically highlight the utility of bathymetric survey using an echo-sounder to assess sediment volume. Our results indicated that the estimated reservoir sediment volume was 6400 m3 leading to a reclamation of 3.2 hectares by layering 0.2 m sediment. The sediment used for reclamation depicts neutral pH (7.3), high organic carbon (2.5%), available phosphorus (9.2 mg/kg) and exchangeable potassium (25 cmol(+)/kg). Garlic (Allium sativum) was planted in the reclaiming abandoned farmland and produced 7.1 t/ha of bulb on average. There is a potential of producing 2–3 horticultural crops per year. Thus, developing methods for scaling up potential farmland reclamation using reservoir sediment would contribute to degraded farmland restoration and food security in Ethiopia and beyond.

Phytochemicals Screening and Antioxidant Effectiveness of Garlic (Allium sativum) from Timor Island

The people of Timor Island only know garlic as a kitchen spice. This research provides new knowledge of the benefits of garlic in the health sector, especially as an inhibitor of free radical that can trigger various degenerative diseases. The aims of this research were to identify secondary metabolites contained in the ethanolic extract of garlic (Allium sativum L.) from Timor Island and to determine its effectiveness in inhibiting free radicals. The method used to test secondary metabolites was phytochemical screening using color reagents. Testing the effectiveness of free radical inhibitors from garlic ethanol extract from Timor Island was carried out in 2 stages: 1.) Determination of DPPH maximum wavelength (λ) and 2.) Measurement of antioxidant activity using DPPH method. The results showed that the ethanolic extract of garlic from Timor Island contained secondary metabolites of flavonoids, phenols and terpenoids. It was also very effective in inhibiting free radicals, with the acquisition of IC50 values &lt;50 ppm which was equal to 9.729 ppm. This research gives some information that can be used for the pharmacological ingredients, i.e. as a natural medicine that safe for the body to be consumed by the people of Timor Island. Moreover that also can impact on demand of garlic in the market. This has a very positive impact on improving the economy of garlic farmers on Timor Island.

THE INFLUENCE OF VARIETY ON THE CONTENT OF BIOACTIVE COMPOUNDS IN GARLIC (ALLIUM SATIVUM L.)

Garlic (Allium sativum L.) is the most important species of the Allium genus and an important vegetable crop throughout the world. Garlic is widely used as an obligatory part in many cooked dishes. It is one of the ancient plants playing one of the most important dietary and medicinal roles in human beings for centuries. Garlic possess many therapeutic properties including antimicrobial, antiviral, antifungal, cardioprotective, neuroprotective, anticarcinogenic, antimutagenic, anti-hypertensive, anti-diabetic and antioxidant. This study has focused on the comparison of the total polyphenols content, the total sulphur content and antioxidant activity of the studied varieties of garlic in the area of Nitra, Slovak Republic. The analyzed samples of garlic were collected at the stage of full maturity. TPC was measured using the spectrophotometric method of Folin-Ciocalteu agents. TPC in studied varieties of garlic were determined in the range 742 mg.kg-1 (Mojmír) to 635 mg.kg-1 (Makoi). The determination of the total sulfur content was based on dry combustion in the presence of oxygen and allows for the quantitative conversion of sulfur to SO2. Statistically significant highest level of TSC recorded in 0.751% (Mojmír) and the lowest level was in 0.523% (Makoi). Antioxidant activity was determined by the spectrophotometric method using a compound DPPH˙ (2.2-diphenyl-1-picryhydrazyl). The highest value of AA was measured in Mojmír (17.7%). The lowest level was observed in Makoi (12.4%). In all studied samples of garlic was confirmed by the strong dependence of the total polyphenols content, total sulfur content and antioxidant activity.

Anti-parasitic activity of garlic (Allium sativum) and onion (Allium cepa) juice against crustacean parasite, Lernantropus kroyeri, found on European sea bass (Dicentrarchus labrax)

The copepod parasite Lernantropus kroyeri impairs the productivity of European sea bass (Dicentrarchus labrax) culture by leading to a direct infection or as a vector for other disease agents. In current conditions, the parasitic infections in mariculture are usually treated with the chemicals, mainly with the pesticides. However, therapeutic chemical use has potential risks on the overall aquatic environment. Thus, the aquaculture sector needs to find alternative substances to the treatment or prophylaxis of the diseases instead of chemicals. In the present study, in vitro exposure tests were performed to elucidate the effects of garlic (Allium sativum) and onion (Allium cepa) on adult L. kroyeri. Female individuals of L. kroyeri were exposed to different concentrations of garlic or onion juice (20, 40, 60, 80 and 100% of the plant juice) for a specified time. Garlic juice exposure at a ratio of 100% resulted in instantaneous death of all individuals in five minutes. Although the killing time varied by the concentration, garlic juice killed the L. kroyeri females within 30 min even at the lowest concentration. Onion juice had lower killing effect at low concentrations, however, higher concentrations of onion juice killed the female parasites within 60 min. Results revealed that garlic and onion juices had killing effect on L. kroyeri females in a time and concentration-dependent manner. It can be concluded that against parasitic copepods alternative solutions can be developed in aquaculture, using these plant juices with emphasising necessity of further in vivo tolerance tests in fish.HighlightsGarlic and onion juices had anti-parasitic effect in vitro on the crustacean parasite, Lernantropus kroyeri found on the gills of European sea bass (Dicentrarchus labrax).After exposure of L. kroyeri to garlic and onion juices, the mortality was time and concentration-dependent.The killing activity of garlic juice for L. kroyeri was stronger than that of onion juice.

Efficacy of Topical Cream of Garlic Extract (Allium Sativum) on Wound Healing in Experimental Mice using Aa Acute &gt;Wound Modeling: Determination of Expresión of Tumor Necrotic Factor (TNF-α)

Garlic (Allium sativum) is one of the ethnomedicinal plants used by people in the tropical region. Its bulb is commonly utilized as traditional medicine for wound healing, particularly in the developing world. This study analyzed the efficacy of topical cream of garlic extract (Allium sativum) on the expression of tumor necrotic factor (TNF-α) and wound closure as the parameters of wound healing in the experimental mice using an acute wound modeling. The research design was a randomized post test control group design. The study was conducted at 4 labolatories, i.e., Biofarmaka Laboratory at the Center for Health Education Research at the Hasanuddin University for the preparation of topical cream of garlic bulb extract, Animal Laboratory of the Medicine Faculty of the Hasanuddin University for the care and treatment of the experimental mice, Histopathology Laboratory of the Research Center for Veterinary Sciences in Maros for the preparation of histopatological slides. A total of mice were 54 mice (Rattus novergicus) of wistar strain by making circular excision wound on dorsal with the diameter of 8 mm using punc byopsi. The experimental mice were divided into 3 groups that include the negative control group by treating 0.9% NaCl, the positive control group by treating 3% oxytetracycline and the treatment group by treating 10% topical cream of garlic extract. Wound tissues of mice were microscopically observed on day 3, day 7 and day 14 respectively. Results of the study proved that wound healing by using topical cream of garlic extract (Allium sativum) showed the highest effect in reducing the expression of tumor necrotic factor (TNF-α) and the lowest diameter of wound in the experimental mice compared to the control groups.

Effect of crushing and heating on the formation of volatile organosulfur compounds in garlic

ABSTRACTThe amount of volatile bioactive compounds of garlic (Allium sativum L.) can be modified by techniques that involve disintegration of cells or/and heat transfer. In this study, after crushing of garlic, the amount of organic sulfides increased in the headspace in the function of time (P < 0.05), while the amount of allicin was not influenced (P = 0.249). Increment of heating temperature enhanced the formation of vinyldithiins, diallyl sulfide (DAMS) and methyl propenyl disulfide (MPDS) but had no significant effect (P ≥ 0.05) on the rate of allicin formation. Heat treatment of garlic at 80°C for 10 min hampered the post-treatment formation of most organic sulfides at room temperature, while heating at 60°C for 10 min did not hamper completely their formation during storage at room temperature. Cooking intact garlic cloves resulted in much less organosulfur compounds than crushed garlic of the same weight (P < 0.001).

Relationship among the potato rot nematode, Ditylenchus destructor, densities in soil, root and garlic (Allium sativum) bulbs, and rot damage in stored garlic bulbs

Summary The potato rot nematode, Ditylenchus destructor, threatens garlic production in Japan. The objectives of this study were to determine the relationships of D. destructor densities in soil, garlic roots and outer skins of garlic bulbs, and damage to bulbs that rot during storage. Ditylenchus destructor densities were evaluated with the real-time PCR method. There was a significant positive correlation between D. destructor densities in soil at planting and those in the outer skin of garlic bulbs at harvest in 2016, but not in 2017. Ditylenchus destructor densities in outer skins at harvest were consistently low when those in roots at harvest were lower than 80 ind. (0.05 g)−1. No damage to garlic bulbs after storage was observed when D. destructor densities in outer skins were lower than 300 ind. (0.05 g)−1. These results indicate that D. destructor densities in roots and outer skins may be a good indicator to estimate nematode damage to garlic bulbs after storage.

Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L.

Symptoms of cloves rot of A. ampeloprasum were noticed during 2018 in storage conditions. 16 isolates were obtained (AMP1-AMP16) and according to morphological and cultural characteristics they belong to F. proliferatum (sex. stage Gibberella intermedia), species of Gibberella fujikuroi complex. To confirm morphological identifica?tion, total genomic DNA was extracted from mycelium of the 16 isolates by amplification of TEF-1? gene, using polymerase chain reaction (PCR) that was performed with the primer pair EF1 and EF2. Results presented in this article clearly indicated that the new host of Fusarium proliferatum as the causal agent of cloves rot is ?elephant garlic? Allium ampeloprasum. Pathogenicity test was confirmed on Allium ampeloprasum cloves. Pathogenicity assays revealed that all isolates caused symptoms on tested Allium spp., like naturally infected cloves.