The attachment of certain Agrobacterium species to moss protonema was examined by scanning electron microscopy. The bacteria cultured with the moss adhered to the protonemal filaments of Pylaisiella selwynii and Funaria hygrometrica throughout the fixation and washing procedure prior to examination. The number of bacteria adhering per unit length of filament did not correlate with the ability of four species (A. tumefaciens, A. rubi, A. rhizogenes, A. radiobacter) to induce gametophore formation in P. selwynii. Little difference was found between the number of A. tumefaciens on P. selwynii or on F. hygrometrica, and although this bacterial species induced gametophores on P. selwynii, it had no effect on F. hygrometrica. A. rhizogenes, which is capable of inducing rhizoid formation on both P. selwynii and F. hygrometrica without physical attachment, did associate with filaments of both mosses. Larger numbers of A. tumefaciens and A. rubi, which induce gametophores or callus, were associated with the spore and germ tube of P. selwynii than were A. rhizogenes or A. radiobacter which do not initiate these changes. These results suggest that the germ tube may constitute the site where effective attachment occurs. The number of A. tumefaciens adhering to P. selwynii was greatest at 2–6 h after addition and then decreased by 26 h, raising the possibility that some bacteria lyse following attachment. None of the bacteria adhered to the gametophore walls of either moss, indicating that a change in cell wall surface may occur. The four species of Agrobacterium differed in their orientation when adhering to the moss. These results suggest that the process of bacterial adherence to higher plant cells in Agrobacterium infection is similar to that which we observe in mosses and which leads to developmental changes.