Most of the theoretical, hypothetical, and controversial aspects of the action of adrenergic blocking agents upon transmission to sweat glands and, to some extent, elsewhere in the cat have been reported previously. 2 Thus the present account of action by Hydergine? (an equal admixture of dihydroergocristine, dihydroergocornine, and dihydroergokryptine, Sandoz) and by dihydroergotamine is relieved of much of the burden of discussion. Evidence exists (based upon observed actions of acetylcholine, adrenaline, noradrenaline, atropine, phenoxybenzamine, bretylium, and guanethidine) to the effect that transmission from sudomotor nerve to sweat gland cells (in the cat at least) may be a dual, sequential (cholinergic-adrenergic in that order) affair. Disagreement arises by reason of questions concerning the specificity of alpha-adrenergic blocking agents, dosage, conflicting evidence concerning action or inaction of these agents at given sites (specifically at the sudomotor junction), infeasibility, for one reason or another, of experimental approach to obtain some desirable collateral evidence, and, unfortunately, preconception. The preparations were cats anesthetized by the use of intraperitoneal chloralose (40 mg/kg) and urethane (0.8 mg/kg) mixture. Zinc-zinc sulfate electrodes were placed one upon the surface of the central footpad, the other subcutaneously at a short distance from the first. The foot, so fitted with electrodes, was bound with vinyl plastic tape to hold the electrodes in place and to provide a barrier to evaporative drying of the electrode paste. A Sanborn recorder and d-c amplifiers were employed to display sweat gland action potential (galvanic skin) responses to sudomotor stimulation; this last was achieved through electrodes applied to the proximally severed, isolated, plantar nerves submerged in a mineral oil bath. Hydergine? and dihydroergotamine were injected slowly, intravenously, in doses usually of I mg/kg. But, to see what would happen, doses occasionally were increased to as much as 2.5 mg/kg. However, the results presented herein all pertain to experiments utilizing the minimal dose. Figure 1 illustrates the result of an experiment in which Hydergine? (1 mg/kg) was injected intravenously after response to stimulation had become stable and during the period indicated by the bar beneath the uppermost record. The three rows of recordings are continuous but for two responses lost, in preparing the figure, between the top and middle rows and again between the middle and bottom rows. A time bar between the middle and bottom rows of responses indicates five minutes. Action upon sudomotor transmission by dihydroergotamine is similar to that by Hydergine?. Separate consideration of action by the two agents, therefore, is not warranted, nor is what would virtually amount to duplication of illustration were the action by dihydroergotamine upon transmission to be exemplified. 1.15
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