G1and G2 Research Articles

Effect of Vitamins A, E, D3 Supplemented with Omega3 and Vitamins A, E, D3 (without Omega -3) on Biochemical Blood Parameters of Females Awassi Lamb

This study was conducted in the animal field of the University of Anbar College of Agriculture from 15/December/2021 to 15/February/2022. Twelve Iraqi Awassi female lambs were used in the experiment, at ranged of ages 2-3 months with average body weight 16.737 kg. The experimental animals were randomly divided into three groups, each group consisted of four lambs. The first group (G1) was considered the control group and left without any treatment, while the second group (G2) was treated with omega-3 supplemented with vitamins A, D3 and E of 1.5 CC, whilst the third group (G3) was treated with vitamins A, D3 and E of 1.5 CC by intramuscular injection, the lambs were injected with 6 times, the interval between each injection was 15 days. The lambs were nourished on the same concentrated diet, alfalfa hay, minerals bloke and water given adliptum. The result showed that G2 had significant increased (P≤0.05) on glucose concentration level as comported with G1. G3 had significant increase (P≤0.05) in urea concentration level as comported with G1 and G2. G3 had significant increased (P≤0.05) in Cholesterol and Triglyceride concentration level as comported with G1and G2. G1 had significant increase in LDL concentration level as comported with G2 and G3. T1 had significant increase in AST in G1, G2 and G3 as comported with T2 and T3. In conclusion, that treat a lamb with vitamins A, E, D3 supplemented with omega3 and vitamins A, E, D3 (without omega -3) led to improve lipid profile, liver enzyme activity and public health of lambs.

Treatment of repeat breeding of Iraqi native river buffaloes (Bubalus bubalis) south of Baghdad using different hormonal treatment – a field study

This study aimed to evaluate the effects of hormonal treatment ( GnRH and PGF2α) with and without antibiotics in the treatment of repeat breeders (RB) of Iraqi native river buffaloes (Bubalus bubalis) in private dairy farms located in the south of Baghdad for improving the reproductive performance in addition to find the ratio of repeat breeder. The study included (112) Buffalo cows suffering from repeat breeding for more than three unfertile insemination estrus cycles. Buffalo cows were divided into 4 groups according to programs treatment (G1=PGF2α, G2=GnRH , G3= PGF2α+GnRH and G4=PGF2α+Iodine+Oxytetracyclen) respectively.Results of the study revealed that the using of (GnRH and PGF2α) hormones with and without antibiotic (Oxytetracycline+Iodine) are useful in improving the reproductive performance of Buffalo cows. The pregnancy rate after the application of the proposed treatments , measured after (60-65) days by rectal palpation, in the four groups proposed as follows ( 60.0% , 46.6 % , 71.8 % , 75.0 % ) respectively . The pregnancy rates in groups (G3 and G4) were significantly higher than in (G1and G2) groups (P<0.05) and the treatment with (GnRH+PGF2α) hormones or (PGF2α+Iodine+Oxytetracyclen) was more useful in improving the reproductive performance of these animals, the ratio of repeated breeding in Iraqi native river buffaloes (Bubalus bubalis) was about 44.8% (112 out of 250 animal examined in this study). In conclusion, the application of treatments with (GnRH+PGF2α) or (PGF2α+Iodine+Oxytetracyclen) is useful and highly efficient in improving reproductive performance in buffalo cows suffering from repeat breeding.

Circadian Rhythms of Oxidant-Antioxidant Agents, HPA Axis and Protein Carbonyl Content in Serum, Brain and Adrenal Gland Tissues

The aim of this experiment is to study the circadian rhythm of oxidant-antioxidant components in the body. A total of 20 adult male rabbits were divided into 2 groups (G1and G2)and kept in 2 different rooms. Serum was isolated after blood collection at 12 A.M or 12 P.M respectively from G1 and G2.The results reveal a significant elevation in serum GSH, MDA and SOD during night hours. The B-endorphin showed a value of 84.36±1.36 in rabbits serum during night compared with 53.23±1.12 during day hours. Serum ACTH and cortisone concentration during night hours expressed a value of 71.03±0.16 and 24.46±0.38 compared to 52.80±0.37 and 11.80±0.57 during day hours respectively. Protein carbonyl contents shows a regular variation between serum and tissues during day and night hours. It was concluded that the oxidant-antioxidant imbalance lead to such variation between day and night hours due to activities and metabolism. Extensive research is needed to minimize and overlap such stress.

The determination of asteroid H and G phase function parameters using Gaia DR2

ABSTRACT The Gaia mission will provide the scientific community with high-quality observations of asteroids of all categories. The second release of Gaia data (DR2) was published in 2018 and consists of 22 months of observations of 14 099 known Solar system objects, mainly asteroids. The purpose of this work is to obtain a catalogue of phase function parameters (H and G) for all the asteroids that were observed during the Gaia mission and that were published in DR2. For this purpose, we introduce an algorithm capable of building this catalogue from the magnitude and UTC epoch data present in the DR2 data base. Because Gaia will never observe asteroids with a phase angle of 0° (corresponding to opposition), but with phase angles higher than 10°, we added data from ground observations (corresponding to small phase angles) and thus improved the determination of the H and G parameters of the phase function. We also built a catalogue of the parameters of the H, G1 andG2 phase function. We compared our results of the H, G functions with those of the Astorb data base and observed that the level of agreement is satisfactory.

Use of hypertonic glucose (10%) in the prevention of postoperative adhesions in rats.

ABSTRACTPurposeTo evaluate the efficacy of hypertonic glucose (10%), alone or in combination with the corticoid dexamethasone, to prevent peritoneal adhesion following hysterectomy in rats.MethodsForty-two adult rats underwent hysterectomy with peritoneal lavage: G1 – glucose (10%); G2 – glucose (10%) and dexamethasone 3 mg·kg–1; and G3 – physiological saline (PS) 0.9%.ResultsIn the macroscopic analysis after 14 days, G1 had a median score of 1, G2 of 1, and G3 of 2.5 (p < 0.0001), G3 compared to G1 and G2. There was no difference between groups after 28 days. In the microscopic analysis, the median vascular proliferation after 14 days was 2 for G1, 1 for G2, and 3 for G3 (p = 0.0037, G3 vs. G1 and G2). After 28 days, G1 showed a median vascular proliferation score of 2, G2 of 2.5, and G3 of 3 (p < 0.0001, G3 vs. G1 and G2). Regarding the inflammatory reaction after 14 days, G1 had a median score of 2, G2 of 1, and G3 of 3 (p = 0.7916). After 28 days, G1 had a median score of 0.5 (0–1.75), G2 of 1.5, and G3 of 2.5 (p < 0.0001, G3 vs. the others and G2 vs. G1). In the evaluation of fibrosis after 14 days, G1 had a median score of 1, G2 of 1, and G3 of 2.5 (p < 0.0001, G3 vs. G1and G2). After 28 days, G1 had a median fibrosis score of 1, G2: 2, and G3: 2.5 (p < 0.0001), G3 vs. the others andG2 vs. G1).ConclusionsThe use of hypertonic glucose (10%) solution seems to reduce macroscopic and microscopic pelvic adhesions.

Efficacy of Nigella sativa oil and its chitosan loaded nanoparticles on experimental cystic echinoncoccosis with immunological assessment

Background: Cystic echinococcosis is a frequent worldwide disease. Surgery is the main policy oftreatment. The benzimidazoles are the drugs of choice as adjuvant therapy besides surgery, but they havesome drawbacks, so there is a pressing need to develop other safe and cheap drugs such as an herbalextract.Objectives: To increase the efficacy of Nigella sativa oil (NSO) administered either as monotherapy orcombined with albendazole, and using chitosan nanoparticles as a drug delivery system for both drugs.Material and Methods: E. granulosus protoscoleces were obtained by aseptic puncture of hydatidcysts. Mice infected by intraperitoneal inoculation with nearly 1000 protoscoleces per mouse weresubdivided into 7 groups: G2, infected non-treated; G3, treated with albendazole; G4, treated with NSO;G5, treated with NSO and albendazole; G6, treated with albendazole loaded on chitosan nanoparticles;G7, treated with NSO loaded on chitosan nanoparticles; G8, treated with albendazole and NSO loaded onchitosan nanoparticles. G1 included the non-infected, non-treated control mice. Serum was collected forbiochemical and serological assay. NO was measured according to nitric oxide assay colorimetric kit. Thesystemic level of TNF-α and IL-5 was determined in the plasma of mice using commercial enzyme-linkedimmunosorbent assay kits.Results: The results revealed that a significant increase of IL-5 occurred in all treated groups to reach thehighest value in G8. There was a significant decrease of TNF-α in all treated groups in comparison to G1and G2 (P<0.001). Mean level of nitric oxide (NO) was significantly increased in groups loaded on chitosannanoparticles in comparison to G2.Conclusion: Nigella sativa oil showed a promising effect on CE when used alone or in combination withalbendazole and its effect was augmented when loaded on chitosan nanoparticles.

Assessment of sodium thiosulfate neutralizing effect on micro-hardness of dentin treated with sodium hypochlorite

BackgroundThis study aims to evaluate the ability of sodium thiosulfate (STS) to neutralize the adverse effect of sodium hypochlorite (NaOCl) on dentin micro-hardness.MethodsFifty single-rooted teeth were longitudinally sectioned. The samples divided into a control and four sample groups (n = 20). All the samples were immersed in different solutions as follows, Control: Normal saline for 15 min, G1and G2: 2.5% NaOCl for 15 min, G3: 2.5% NaOCl for 15 min, followed by 5% STS for 10 min, G4: Normal saline for 15 min followed by 5% STS for 10 min. All groups except G1 incubated for one week before the test. The micro-hardness of samples was measured. Data were analyzed using the Kruskal–Wallis test for pairwise comparisons. A p value < 0.05 was considered significant.ResultsAll groups showed a significant decrease in the micro-hardness value compared with the control group. NaOCl for one week (G2) reduced the micro-hardness of dentine compared with samples, tested immediately after immersion in NaOCl (G1) (p < 0.05). NaOCl alone (G2) or treated with STS (G3) resulted in a significant decrease in micro-hardness compared with the STS group (G4) (p < 0.05).ConclusionsSTS as a neutralizing agent could not prevent the dentin micro-hardness downturn caused by NaOCl.

AB0988 CLINICAL FEATURES AND ANALYSIS OF MEFV GENE IN 31 PATIENTS WITH FAMILIAL MEDITERRANEAN FEVER (FMF)

Background:FMF is recessive systemic autoinflammatory disorder characterized by recurrent fever, peritonitis, pleuritis, pericarditis and arthritis accompanied with headache and abdominal pain. Mutation of MEFV gene encoding pyrin resulted in inflammasome activation and the uncontrolled production of IL-1β. Overview of pathogenesis, clinical features and management in Japanese patients with FMF had been reported. However, the differences of clinical features between mutated and non-mutated of MEFV still remain unclear.Objectives:We have analyzed 31 Japanese patients with FMF in Gifu district to clarify the association between various clinical features and mutation ofMEFV.Methods:Genomic DNA were purified from white blood cells in 31 FMF patients, and mutated MEFV has been explored. We have analyzedMEFV, TNFRSF1A, MVK and NLRP3 genesin 31 patients with FMF except for 1 patient. Therefore, we excluded another autoinflammatory diseases such as TNF receptor-associated syndrome (TRAPS), mevalonate kinase deficiency and cryopyrin-associated periodic syndrome. Clinical symptoms and laboratory data were analyzed around onset time. Each patient had been treated with colchicine (0.5-2 mg).Results:Characteristics of Patients with FMF (22 female/9 male) were as follows; Onset time were 0-56 years-old (21.4 ±11.8), and Frequencies of clinical symptoms such as periodic fever, headache, abdominal pain, arthralgia, chest pain, cervical lymph nodes swelling, and myalgia were 31/31, 9/31, 8/31, 6/31, 5/31, 3/31 and 1/31, respectively (double symptoms were observed). Patients with FMF were divided into 3 groups as follows; Patients with typical compound heterozygous mutations of MEFV (E148Q /M694I) which indicated exon 10 mutation, were 5 cases (G1). Patients with atypical mutations, except for exon 10, such as 133G&gt;A in 3UTR, exon 1 (E84K), 2 (L110P, E148Q), 3(R202Q, P257L, G304R, P369S, R408Q), 5(S503C) and 9(I591M) were 13 cases (G2). Patients with no mutations in MEFV gene were 12 cases (G3). There were no significant differences of age at first visiting hospital (FV)and onset age of fever attack (O) (FV: 29.0 ± 15.6, 27.1 ± 12.5 years-old (yo) and 34.7 ± 12.7 yo, O: 21.0 ± 17.6 yo, 17.8 ± 12.1 yo and 25.2 ± 6.5). But significant differences in duration of fever attack (D) and frequency of fever attack (FF) between G1 and G2 or G3 were observed as follows; (D: 2.2 ± 0.4 days vs 5.5 ± 3.1 days, P&lt;0.05, and 3.8 ± 1.7 days), FF: 0.72 ± 0.3/month (M), 1.24 ± 1.1/M, and 1.5 ± 0.7/M vs group1, P&lt;0.05), respectively. Laboratory examinations such as WBC, CRP and serum amyloid A (SAA)at fever attack were not significantly different between 3 groups. All of those patients were effective for colchicine treatment except for 2 patients in group 1 because of loss of hair, severe diarrhea and liver dysfunction due to side effects of colchicine. Finally, 4 patients in G1and G2 received canakinumab treatment. Patients withMEFVmutations have no family histories. Mutations of E148Q were found in 12 patients (40%).Conclusion:We have examined association between clinical features and mutations of MEFV in 31 patients in Gifu district, suggesting that duration of fever attacks and frequency of fever attacks in G1 are significantly shorter than G2 and G3, respectively in Japanese patients with FMF. Mutations of E148Q in exon 2 were observed in 16-23 % of normal Japanese patients, indicating that E148Q is the polymorphism or accelerating factor.Disclosure of Interests:None declared

EFFECT OF GRAZING TIME ON PRODUCTIVE PERFORMANCE OF GOAT IN HALAIB – SHALATEEN REGION

EFFECT OF GRAZING TIME ON PRODUCTIVE PERFORMANCE OF GOAT IN HALAIB – SHALATEEN REGION

COMPARISON OF QUALITY CONTROL PARAMETERS OF DIAGNOSTIC X-RAY MACHINES IN GOVERNMENT, MISSION AND PRIVATE OWNED FACILITIES IN JOS, NIGERIA

Due to the availability and frequent use of x-ray machine in the diagnoses of diseases and treatment of diseases in most of our hospitals and radio-diagnostic centers in Nigeria, the need to assess the quality control parameters and level of compliance of the x-ray machines cannot be over emphasized. The purpose of this research is to compare the result of four Quality Control test parameters of government (G1and G2), mission (M1 and M2) and private owned (P1and P2) x-ray machines of the radio-diagnostic facilities to ascertain the level of compliance among them with the set reference tolerance limits. Four Quality Control test parameters; kVp accuracy, congruence between optical and x-ray field, beam alignment and tube filtration was assessed from the measurement carried out using Gammex Radiologic kit with Gammex 330 digital kV meter, Gammex 115A half value layer set, collimator test tool model 161B and the beam alignment test tool model 162A.The result of kVp accuracy show that G1,G2,M2 and P1 all had values ranging from 2.30% - 3.14% within the set tolerance limit of ± 5% while M1 and P2 had values above the limit. In congruence between optical and x-ray field with tolerance limit of ±2%, only P2 was above limit, while in perpendicularity G2 and P2 were above the set limit of 1.5o. Tube filtration all the studied x-ray machines failed the test indicate absorption of excess low energy dose for patient. This research therefore reveals that the government and mission x-ray machines have better quality control programme that the private owned.&#x0D; Ike-Ogbonna, M. I. | Department of Physics, University of Jos, Plateau State, Nigeria

Pulmonary Rehabilitation Effects on Heart Rate Recovery in Restrictive Lung Disease Patients

Objective: The study aimed to evaluate heart rate recovery in subjects with restrictive lung disease (RLD) and healthy age matched subjects before and after a rehabilitation program (RP). Methods: This is a cross-sectional study on subjects of both genders, with over 40 years old with and without any diagnosis of restrictive lung disease and who were able to perform physical tasks were included in the study. They were evaluated for sociodemographic profile, lifestyle (ILP), Framingham score, physical capacity (6MWT and treadmill incremental testing) and heart rate recovery before and after RP. Results: 65 subjects were assigned into either G1 (patients with RLD, n=26) or G2 (healthy subjects, n= 39). Initially, patients with RLD increased their heart rate (HR) from 79 to 120bpm, and after the recovery post-exercise, the first minute they reduced HR by nine beats. In the fifth minute after the exercise, HR returned to baseline values. After the RP, mean HR was 71bpm and 79bpm in G1 and G2, respectively. Increased mean 6MWT peak HR in both the groups was seen (110bpm and 120bpm, G1 and G2, respectively). In the first minute of resting, mean HR decreased to 86bpm (- 24bpm) and 72 (-48bpm) in G1 and G2, respectively. In the fifth minute after exercise, HR recovery in both the groups was complete. Conclusion: The RP was effective in improving the first-minute heart rate recovery in patients with restrictive lung disease and, there was an inverse correlation of heart rate recovery with disease severity.

An Approach Study for Reducing the Early Embryonic Loss in Egyptian Buffalo-cows

This study evaluated the efficacy of a single gonadotropin-releasing hormone (GnRH) agonist on day 12 or human chorionic gonadotropin (hCG) on day 7 post-mating on pregnancy rate (PR) of repeat breeder buffaloes. Buffaloes (n=21) in heat were naturally mated, and divided into three groups (7 in each). Animals in the 1^st group (G1) were i.m. injected with 5 ml GnRH on 12-day post-mating, while those in G2 were i.m. injected with 1500 IU of hCG on day 7 post-mating. In G3, animals were kept as control. Results show that PR was higher (P<0.05) in G1 (71.428) and G2 (57.142) than G3 (28.571). The peak of serum P4 was recorded (P<0.05) on day 15 in G2, and day 18 in G1 and G3. On days 21 and 24 post-mating, P4 was the highest (P<0.05) in G1, followed by G2, and the lowest in G3. Average CL diameter increased (P<0.05) CL in G1and G2 compared with G3 on days 15 and 18 post-mating. The concentration of P4 was higher in pregnant than in non-pregnant animals on different post-mating days, regardless of treatment. The concentration of P4 was the highest in pregnant of G2, and the lowest in non-pregnants of G3, being more obvious from 18 to 24-day post-mating. Injection of repeat breeder lactating buffaloes with 5 ml GnRH or 1500 IU hCG on day 12 and 7 post-mating, respectively, improved P4 production and pregnancy rate. GnRH treatment reduced the early embryonic loss showing the highest pregnancy rate to increase the farmer economy.

Effectiveness of Hydrogen Peroxide on Reducing Irrigation Water ‎Quantities, Pests and Enhancing Kohlrabi Production

This study was carried out at Faculty of Agricultural - Suez Canal University which located in north eastern Egypt, within the Governorate of Ismailia. The experiment was conducted to assess the influence of hydrogen peroxide on reducing irrigation water quantities, Mite infestation and kohlrabi Production. Thus; the aim of this study is to monitoring the influence of three treatments hydrogen peroxide (G1, G2 and G3) with average (5mL/plant, 10mL/plant and 0mL/plant [without hydrogen peroxide]) respectively, and three treatments for irrigation water quantity [Q1, Q2 and Q3] with average amount of water (180, 270 and 360mm) respectively on yield production, growth parameters, leaf area index, mites, insects density, and irrigation water uses efficiency. The highest yield value has recorded with Treatment (G1) by (5427.3 Kg.Fed-1). Which is a significant value comparing with other treatment (G2 and G3) which obtained a low value by (3721.7 and 4325.07 Kg.Fed-1) respectively. In addition; the result of irrigation water use efficiency recorded a best value with (G1) by 5.62, 3.25and 4.16(Kg.m -3 ) under Q1, Q2 and Q3 respectively. Thus; the irrigation water use efficiency obtains a highest mean value by (4.43 Kg.m -3 ) with using hydrogen peroxide treatment (G1). data indicated that there is a significant influence for hydrogen peroxide with (G1) treatment on Euseius scutalis mite which recorded a highest dentistry by (3.78 N/in 2 ) but the lowest value obtained with ( G3) by (1.36 N/in 2 ). In contrary; there is not any significant impact on Euseius scutalis density with other treatment (Q1 and Q3) which got (2.2 and 2.07 N/in 2 ) respectively. Moreover; hydrogen peroxide treatments (G1and G2) did not have a significant influence on the other mite ( Amblyseius swirskii) comparing with (G3) treatment. Further; using one doses from (5mL/plant) hydrogen peroxide reducing the density of insects ( Aphis gossypii and Thrips tabaci) by 50 % comparing with the other value which did not adding a hydrogen peroxide. In addition; the insects increased when reducing the amount of water from 360mm to 270mm by 50% for Aphis gossypii and 75% for Thrips tabaci. Keywords : Hydrogen Peroxide, water quantities; mite &amp; insects densities; Irrigation water use efficiency and kohlrabi production. DOI : 10.7176/JNSR/9-20-07 Publication date :October 31 st 2019

Hashing to G2 on BLS pairing-friendly curves

When a pairing e : G 1 x G 2 → G T , on an elliptic curve E defined over F q , is exploited in a cryptographic protocol, there is often the need to hash binary strings into G 1 and G 2 . Traditionally, if E admits a twist Ẽ of order d, then G 1 = E (F q )⋂ E [ r ], where r is a prime integer, and G 2 = Ẽ(F q k/d )⋂ Ẽ [ r ], where k is the embedding degree of E w.r.t. r. The standard approach for hashing a binary string into G 1 and G 2 is to map it to general points P∈E ( F q ) and P′ ∈ Ẽ (F q k/d ), and then multiply them by the cofactors c = #E (F q )/ r and c ′ = #Ẽ (F q k/d )/ r respectively. Usually, the multiplication by c′ is computationally expensive. In order to speed up such a computation, two different methods (by Scott et al. and by Fuentes et al. ) have been proposed. In this poster we consider these two methods for BLS pairing-friendly curves having k ∈ {12, 24, 30, 42,48}, providing efficiency comparisons. When k = 42,48, the Fuentes et al. method requires an expensive one-off pre-computation which was infeasible for the computational power at our disposal. In these cases, we theoretically obtain hashing maps that follow Fuentes et al. idea.

Resistance Distance and Kirchhoff Index of $Q$ -Double Join Graphs

Let Q(G) be the graph derived from G by inserting a new vertex into every edge of G and by connecting edges of these new vertices that are on adjacent edges of G, and Q-double join of G, G 1 and G 2 denoted by G Q v{G 1 , G 2 }, is the graph derived from Q(G), G 1 and G 2 , by connecting every old-vertex V (G) of Q(G) with every vertex of G 1 and every new-vertex I(G) of Q(G) with every vertex of G 2 . In this paper, we mainly considered the resistance distance and Kirchhoff index of Q-double join graphs G Q v {G 1 , G 2 } of an r-regular graph G and any two graphs of G 1 and G 2 .

Effect of feeding micronutrient fertilized sorghum hay based diet on nutrient utilization and mineral balance in sheep

The present work was undertaken to study the effect of feeding micro-nutrient rich sorghum hay in the diet of sheep on nutrient intake and its utilization. Twelve adult Jalauni sheep (weighing 39.11±1.02 kg) were randomly divided into 2 groups of 6 animals each. The sheep of control group (G1) were fed on NPK fertilized chopped sorghum hay and crushed barley grain (300g/d) based ration, while the sheep of group G2 were offered NPK + 50% recommended dose of micronutrients (Zn, Mn and Cu 10, 5 and 2.5 kg/ha) + seed priming in 0.05% solution of ZnSO4 for 12 h + VAM inoculated sorghum hay along with barley grain (300g/d) for 30 days. Micro-nutrient (Cu, Zn and Mn) fertilization improved mineral content (ppm) in sorghum hay for Cu (7.47 vs 9.22), Zn (22.69 vs 27.48) and Mn (73.56 vs 102.01). The effective intake of minerals was Cu, 8.71and 10.06, Zn, 26.23 and 30.18; Mn, 62.59 and 86.05 ppm in G1and G2, respectively. The additional mineral supplementation through micronutrient rich sorghum hay had no significant effect on dry matter intake. Similarly, the intakes of digestible crude protein, metabolizable energy and digestibility of DM, CP, NDF, ADF, were also comparable among the groups. However, micro-nutrient (Cu, Zn and Mn) supplementation through fodder sorghum improved the mineral retention (Cu 2.84 vs 3.72 mg/d, Zn 11.72 vs 15.46 mg/d and Mn 26.19 vs 40.28 mg/d) without affecting significantly the absorption coefficient in adult sheep. The apparent absorption and retention of N was positive and comparable between thegroups. Nutrient content (%) in terms of DCP and TDN in both type diets fed to sheep were also similar. Thus, it can be concluded that micro-nutrient (Cu, Zn and Mn) application to fodder sorghum improved the mineral content in the fodder, and feeding of micronutrient fertilized sorghum hay based diet improve the intake and retention of micro-minerals significantly without affecting nutrient intake and nutrient utilization in adult Jalauni sheep.

Classification des tumeurs neuroendocrines pancréatiques : nouveautés introduites par la classification OMS 2017 des tumeurs des organes endocrines et perspectives

The WHO classification of the tumors of endocrine organs, published in July 2017, has introduced significant changes in the classification of pancreatic neuroendocrine tumors, the previous version of which has appeared in 2010, within the WHO classification of the tumors of the digestive system. The main change is the introduction of a new category of well-differentiated neoplasms, neuroendocrine tumors G3, in addition to the previous categories of neuroendocrine tumors G1and G2. The differential diagnosis between neuroendocrine tumors G3 (well-differentiated) and neuroendocrine carcinomas (poorly-differentiated) might be difficult; the authors of the WHO classification therefore suggest the use of a number of immunohistochemical markers to facilitate the distinction between the two entities. The other changes are: (a) the modification of the threshold between neuroendocrine tumors G1and G2, now set at 3%; (b) the terminology used for mixed tumors: the previous term mixed adeno-neuroendocrine carcinoma (MANEC) is substituted by the term mixed neuroendocrine-non neuroendocrine neoplasm (MiNEN). Finally, the recommendations for Ki-67 index evaluation are actualized. Even if these changes only concern, stricto sensu, the neuroendocrine tumors of pancreatic location, they will probably be applied, de facto, for all digestive neuroendocrine tumors. The revision of the histological classification of pancreatic neuroendocrine tumors coincides with the revision of their UICC TNM staging; significant changes have been made in the criteria for T3and T4stages. Our professional practices have to take into account all these modifications.

Biological Evaluation of Microwave Defatted Black Rice Bran in Hypercholsterolemic Rats.

This study was performed to investigate the effect of feeding on Microwave defatted black rice bran (MDBLRB) in hypercholesterolemic rats . Chemical composition and total phenolic compounds (TPC) of microwave defatted black rice bran (MDBLRB) and microwave full fat black rice bran (MFBLRB) were determined . Results revealed that (MDBLRB) protein, ash, fiber and carbohydrates contents of (18.20, 0.70, 10.1, 13.7 and 71.00) were higher than those of MFBLRB. Also, total phenolic compounds (TPC) being 561 as mg Tannic acid equivalent/kg for (MDBLRB) in compare with 486 as mg Tannic acid equivalent/kg for MFBLRB, obtained results illustrated that substitution of feeding hypercholesterolemic diet with MDBLRB led to improvement the High lipoprotein cholesterol HDL-C. Furthermore, hypercholesterolemic diet with (MDBLRB) replacement at 75% and 100% also recorded the best and nearest of HDL-C to the negative control. It was observed also that LDL- cholesterol value of negative control diets (G1and G2) were 46.78 and 45.15 mg/dl, but also the value of the hypercholesterolemic in control (G3) was 198.77mg/dl. On the other hand, the LDL cholesterol of rats fed on hypercholesterolemic diet substitution with MDBLRB 25,50.75 and 100% (G4, G5, G6 and G7) being 50.67, 47.85, 46.28 and 41.73 mg/dl, respectively. At the final of experiment (10 weeks), ALT was significantly increased for the hypercholesterolemic control (G3) was 50.30 U/L, while negative control (G1 and G2) were 25.80 and 24.20 U/L respectively. Feeding on hypercholesterolemic diets substituted with MDBLRB (G4, G5, G6 and G7) led to a more reduction at level 25, 50, 75 and 100% were 38.52, 36.61, 34.34 and 32.55 U/L, respectively comparing with hypercholesterolemic control (G3). Briefly it be could conclude that MDBLRB has pronounced effect in lowering cholesterol serum levels and may be useful for patients suffering from liver and cholesterol diseases

Response of cowpea genotypes to water stress in temperate climatic conditions

Mitigation of global climate change impact on the agricultural production is the major priorities in future research. Cowpea as a drought tolerant plant is interesting for growing in semi-arid climate of the Vojvodina region. The effect of water stress on yield of cultivated plants can be obtained by calculating the yield response factor (Ky) which represents the ratio between the relative evapotranspiration deficit (1-ETa/ETm) and the relative decline in yield (1-Ya/Ym). The values of Ky ranged from 0.91 to 1.17 for genotype G1 and G2 respectively. Genotype G1, with a value lower than 1 of Ky, shows a good tolerance to water deficit, on the contrary, genotype G2, with a greater Ky than 1, expresses some sensitivity to water stress. Obtained results will be used in breeding programs to develop cowpea cultivars tolerant to stressful conditions, primarily to water stress, as well as more productive in water use.

Histone Deacetylase 10 Regulates the Cell Cycle G2/M Phase Transition via a Novel Let-7-HMGA2-Cyclin A2 Pathway.

Histone deacetylase (HDAC) inhibition leads to cell cycle arrest in G1 and G2, suggesting HDACs as therapeutic targets for cancer and diseases linked to abnormal cell growth and proliferation. Many HDACs are transcriptional repressors. Some may alter cell cycle progression by deacetylating histones and repressing transcription of key cell cycle regulatory genes. Here, we report that HDAC10 regulates the cell cycle via modulation of cyclin A2 expression, and cyclin A2 overexpression rescues HDAC10 knockdown-induced G2/M transition arrest. HDAC10 regulates cyclin A2 expression by deacetylating histones near the let-7 promoter, thereby repressing transcription. In HDAC10 knockdown cells, let-7f and microRNA 98 (miR-98) were upregulated and the let-7 family target, HMGA2, was downregulated. HMGA2 loss resulted in enrichment of the transcriptional repressor E4F at the cyclin A2 promoter. These findings support a role for HDACs in cell cycle regulation, reveal a novel mechanism of HDAC10 action, and extend the potential of HDACs as targets in diseases of cell cycle dysregulation.