Singlet Oxygen (SO) generation is the core chemical process of many medical and industrial applications. Designing efficient SO-Generating (SOG) agents is a major research focus for its utility in photodynamic therapy, wastewater treatment, material science, and organic synthesis. Photosensitization of ambient oxygen using organic dyes is an efficient approach to generating SO, though their efficiency is often severely hampered due to aggregation-induced deactivation. In this manuscript, we present the utility of bile salt micelle as supramolecular host for promoting SOG in aqueous media for a BODIPY dye, which was otherwise highly inactive. The SOG efficiency in test media was probed by monitoring the oxidation of 1,5-Dihydroxy Naphthalene (DHN) to corresponding quinone (juglone) through spectroscopic and chromatographic methods. In this study, we investigated the use of bile salt micelles as a supramolecular platform for enhancing the SOG efficiency of a BODIPY dye in aqueous media through complexation. Our results show that sodium cholate micelles can solubilize the hydrophobic dye and enhance its ability to generate singlet oxygen, while traditionally used macrocyclic hosts such as Β- and γ-cyclodextrin complexation was found to be far less efficient. Computational modeling revealed that the bile salt micelles form an aggregated complex structure with the dye, leading to improved supramolecular interactions and photophysical properties. This work demonstrates the potential of bile salt micelles as a natural and safe platform for enhancing the activity of nonpolar dyes for PDT applications.