Abstract: An important part in detecting and diagnosing leukaemia involves the microscopic examination of a patient’s blood sample. The type of malignant cells called the blasts is frequently determined by morphological alterations in White Blood Cells (WBCs). Real-time procedures are time-consuming, error-prone, and expensive. In order to overcome this problem the features such as the colour, shapes and texture can be extracted from the original image. This paper deals with the segmentation techniques. The segmentation process in automated approach is developed using k-means clustering (KMC) and Hidden-Markov Random Field (HMRF). This is also termed as Refined Segmentation (Re-Se). This analysis includes Aspirate Smear Images (ASI). First cell segmentation using a KMC is performed, then a Cell Image Representing Model (CIRM) using HMRF is build, estimating prototype variables using Expectation Maximization (EM) ratio, iterating until the best value is found, and finally achieving next phase called Refined Segmentation (Re-Se). Then, features including texture, geometry, colour, and statistical information are retrieved and used to categorize leukaemia cells from WBCs, by using Fuzzy Rule-Based Decision Support System (FRDSS). By incorporating more feature extraction techniques, the suggested method improves a blast of most immature cells identification for the Acute Myeloid Leukemia (AML) detection
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