Considering uncertainties associated with sustainable energy sources in modern power systems, one of the main obstacles for load frequency control (LFC) is that various publications regard the parameters of FOPID controllers as fixed after optimisation. To tackle this challenge, an adaptive control structure may be a viable option for LFC. In this paper, in a two-zone hybrid power system that includes a thermal power plant, green energy sources, fuel cells, and plug-in electric vehicles, an optimal self-tuning fractional order fuzzy (OSTFOF) controller for LFC is proposed. Pathfinder algorithm (PFA) has been utilised for optimal tuning of parameters of the OSTFOF controller, including the upper bands of output membership functions, the input scaling factors, and the order of integral and derivative operators. In the proposed controller, the values of proportional, integral, and derivative gains are adaptively obtained. In the thermal power plant, non-linear factors, such as governor dead band (GDB) and generation rate constraint (GRC), are considered. Four different scenarios have been considered to evaluate the performance of the proposed OSTFOF controller, and results have been compared with PI, PID, and FOPID controllers. The simulations’ results indicate the excellent performance indices of the suggested OSTFOF controller compared to other controllers.