Projections of streamflow under future climate are essential for developing adaptation strategies in the water and related sectors. This paper presents nationwide streamflow projections for Australia informed by climate change signals in CMIP6 GCMs and compares the projections with those from CMIP5 GCMs. The modelled future runoff projections driven by CMIP5 and CMIP6 GCMs are relatively similar for far southern Australia. The median projection is a 50% reduction in mean annual runoff in far southwest Australia and 20% reduction in far southeast Australia by 2046–2075 relative to 1976–2015 under the high SSP5-8.5/RCP8.5 global warming scenario. The vast majority of CMIP5 and CMIP6 GCMs project less winter rainfall across Australia. As most of the runoff in far southern Australia occurs in winter and spring, the lower winter rainfall translates to a significant reduction in annual runoff. It is therefore prudent to plan and manage for a reduction in future water resources, particularly in the densely populated and important agricultural regions in southeast Australia. There is less agreement between GCMs in the summer rainfall projection, with the CMIP6 GCMs generally projecting a wetter future (or smaller decrease in rainfall) than the CMIP5 GCMs. The modelled median projection for mean annual runoff is a 10% reduction in the south-east coast, 5% reduction in the north-east coast and little change in northern Australia. More GCMs project an increase rather than a decrease in the interannual variability of rainfall, and this would further amplify multi-year hydrological droughts.