Future Experiments Research Articles

Meson spectroscopy from spectral densities in lattice gauge theories

Spectral densities encode nonperturbative information that enters the calculation of a plethora of physical observables in strongly coupled field theories. Phenomenological applications encompass aspects of standard-model hadronic physics, observable at current colliders, as well as correlation functions characterizing new physics proposals, testable in future experiments. By making use of numerical data produced in a Sp(4) lattice gauge theory with matter transforming in an admixture of fundamental and 2-index antisymmetric representations of the gauge group, we perform a systematic study to demonstrate the effectiveness of recent technological progress in the reconstruction of spectral densities.To this purpose, we write and test new software packages that use energy-smeared spectral densities to analyze the mass spectrum of mesons. We assess the effectiveness of different smearing kernels and optimize the smearing parameters to the characteristics of available lattice ensembles. For concreteness, we analyze the Sp(4) lattice gauge theory with matter transforming in an admixture of fundamental and 2-index antisymmetric representations of the gauge group. We generate new ensembles for the theory in consideration, with lattices that have a longer extent in the time direction with respect to the spatial ones. We run our tests on these ensembles, obtaining new results about the spectrum of light mesons and their excitations. We make available our algorithm and software for the extraction of spectral densities, that can be applied to theories with other gauge groups, including the theory of strong interactions (QCD) governing hadronic physics in the standard model. Published by the American Physical Society 2024

Electric dipole moments as probes of the RD(*) anomaly

The measurements of the lepton flavor universality (LFU) in B(B¯→D(*)lν¯) indicate a significant deviation from the standard model prediction at a 3–4σ level, revealing a violation of the LFU (RD(*) anomaly). It is known that the RD(*) anomaly can be easily accommodated by an SU(2)L-singlet vector leptoquark (LQ) coupled primarily to third-generation fermions, whose existence is further motivated by a partial gauge unification. In general, such a LQ naturally leads to additional CP-violating phases in the LQ interactions. In this paper, we point out that the current RD(*) anomaly prefers the CP-violating interaction although B(B¯→D(*)lν¯) are CP-conserving observables. The CP-violating LQ predicts a substantial size of the bottom-quark electric dipole moment (EDM), the chromo-EDM, and also the tau-lepton EDM. Eventually at low energy, the nucleon and electron EDMs are radiatively induced. Therefore, we conclude that the RD(*) anomaly with the SU(2)L-singlet vector LQ provides unique predictions: neutron and proton EDMs with opposite signs and a magnitude of O(10−27) e cm, and suppressed electron EDM. Furthermore, we show that a similar EDM pattern is predicted in an SU(2)L-doublet scalar LQ scenario that can accommodate the RD(*) anomaly as well. These EDM signals could serve as crucial indicators in future experiments. Published by the American Physical Society 2024

New high-precision measurement system for electron–positron pairs from sub-GeV/GeV gamma-rays in the emulsion telescope

The GRAINE project observes cosmic gamma-rays, using a balloon-borne emulsion-film-based telescope in the sub-GeV/GeV energy band. We reported in our previous balloon experiment in 2018, GRAINE2018, the detection of the known brightest source, Vela pulsar, with the highest angular resolution ever reported in an energy range of >80MeV. However, the emulsion scanning system used in the experiment was designed to achieve a high-speed scanning, and it was not accurate enough to ensure the optimum spatial resolution of the emulsion film and limited the performance. Here, we report a new high-precision scanning system that can be used to greatly improve the observation result of GRAINE2018 and also be employed in future experiments. The scanning system involves a new algorithm that recognizes each silver grain on an emulsion film and is capable of measuring tracks with a positional resolution for the passing points of tracks of almost the same as the intrinsic resolution of nuclear emulsion film (∼70 nm). This resolution is approximately one order of magnitude smaller than that obtained with the high-speed scanning system. With this scanning system, an angular resolution for gamma-rays of 0.1° at 1GeV is expected to be achieved. Furthermore, we successfully combine the new high-precision scanning system with the existing high-speed scanning system, enabling the high-speed and high-precision measurements. Employing these techniques, we reanalyze the gamma-ray events detected previously by only the high-speed scanning system in GRAINE2018 and obtain an about three times higher angular resolution (0.22°) in the 500–700MeV energy range. Adopting this technique in future observations may provide new insights into the gamma-ray emission from the Galactic center region and may realize polarization measurements of high-energy cosmic gamma-rays.

QCLUE: a quantum clustering algorithm for multi-dimensional datasets

Clustering algorithms are at the basis of several technological applications, and are fueling the development of rapidly evolving fields such as machine learning. In the recent past, however, it has become apparent that they face challenges stemming from datasets that span more spatial dimensions. In fact, the best-performing clustering algorithms scale linearly in the number of points, but quadratically with respect to the local density of points. In this work, we introduce qCLUE, a quantum clustering algorithm that scales linearly in both the number of points and their density. qCLUE is inspired by CLUE, an algorithm developed to address the challenging time and memory budgets of Event Reconstruction (ER) in future High-Energy Physics experiments. As such, qCLUE marries decades of development with the quadratic speedup provided by quantum computers. We numerically test qCLUE in several scenarios, demonstrating its effectiveness and proving it to be a promising route to handle complex data analysis tasks – especially in high-dimensional datasets with high densities of points.

Modular representations emerge in neural networks trained to perform context-dependent tasks.

The brain has large-scale modular structure in the form of brain regions, which are thought to arise from constraints on connectivity and the physical geometry of the cortical sheet. In contrast, experimental and theoretical work has argued both for and against the existence of specialized sub-populations of neurons (modules) within single brain regions. By studying artificial neural networks, we show that this local modularity emerges to support context-dependent behavior, but only when the input is low-dimensional. No anatomical constraints are required. We also show when modular specialization emerges at the population level (different modules correspond to orthogonal subspaces). Modularity yields abstract representations, allows for rapid learning and generalization on novel tasks, and facilitates the rapid learning of related contexts. Non-modular representations facilitate the rapid learning of unrelated contexts. Our findings reconcile conflicting experimental results and make predictions for future experiments.

Estimates of natural frequencies for nuclear vibration, and an assessment of the feasibility of selective ultrasound ablation of cancer cells

Selective ablation of cancer cells by ultrasound would be transformative for cancer therapy, but has not yet been possible. A key challenge is that cancerous and non-cancerous cells typically have similar acoustic impedance and are thus indistinguishable as materials in their responses to ultrasound. However, in certain cancers, cytoskeletal and nuclear lamin structures differ between healthy and malignant cells, opening the possibility of exploiting structural differences that manifest as different vibrational responses. To assess the possibility that the nuclei of certain cancerous cells might vibrate at different frequencies, we measured sizes and effective indentation moduli of a range of cancerous and non-cancerous cells from several cell lines and regions of the brain, and estimated the natural frequencies for nuclear vibration. Results suggest a potential difference in natural frequency for nuclear vibration between certain cancerous and non-cancerous cells, on the order of tens of kHz. This gap is potentially sufficient for selective ablation and motivates future experimentation on these specific cell types.

Through the looking glass into the dark dimension: Searching for bulk black hole dark matter with microlensing of [formula omitted]-ray pulsars

Primordial black holes (PBHs) hidden in the incredible bulk of the dark dimension could escape constraints from non-observation of their Hawking radiation. Since these five-dimensional (5D) PBHs are bigger, colder, and longer-lived than usual 4D PBHs of the same mass M, they could make all cosmological dark matter if 1011≲M/g≲1021, i.e., extending the 4D allowed region far down the asteroid-mass window. We show that these evasive PBHs could be search for by measuring their X-ray microlensing events from faraway pulsars. We also show that future X-ray microlensing experiments will be able to probe the interesting range (1016.5≲M/g≲1017.5g) where an all dark matter interpretation in terms of 4D Schwarzschild PBHs is excluded by the non-observation of their Hawking radiation.

Prevalence and abundance of plant-parasitic nematodes associated with corn (Zea mays L.) in Anuradhapura, Sri Lanka  

Plant parasitic nematodes (PPN) are important pests of corn (Zea mays L.) Globally, while corn production has recently expanded in Sri Lanka. The population abundance, distribution, and pathological effects of nematodes that adversely affect corn production in Sri Lanka are poorly understood. This study was conducted to identify the prevalence of major PPN genera, population abundance, and their reproduction associated with corn fields in the Anuradhapura district. Over 92% of the corn fields were positive for PPN from the fields sampled in February 2021. Major PPN genera identified were Pratylenchus (71.4%) Helicotylenchus (28.6%), Meloidogyne (21.4%), Criconemella (21.4%), and Hoplolaimus (35.7%). The mean population of the Pratylenchus was 2020 kg-1 of soil, which was above the threshold level (1000 Pratylenchus kg-1 of soil), while other genera were lower than the threshold. All genera, except Meloidogyne, were observed from the intensive sampling from the selected fields during the next cropping season from November 2021 (seedling stage) to February 2022 (harvesting stage). Pratylenchus abundance in all the fields was lower than the threshold at the seedling stage and it was 2 to 10 times higher during the harvesting stage, except in Kelenikawewa where the initial Pratylenchus population (1865 kg-1 of soil) was also above the threshold. According to our results, Pratylenchus have the potential to impact corn yield in Anuradhapura. Future field experiments will determine the impact of PPN on corn production in Sri Lanka.

Mathematical modeling suggests heterogeneous replication of Mycobacterium tuberculosis in rabbits.

How quickly Mycobacterium tuberculosis ( Mtb ) replicates and dies in lungs of infected individuals is likely to determine the outcome of the infection - either control/clearance of the bacteria by the host immune response or progression to active disease, tuberculosis ( TB ). And yet, only a few studies, primarily in mice, rigorously estimated the rates of Mtb replication and death during an in vivo infection. We infected rabbits with a novel clinical isolate of Mtb carrying an unstable, "replication clock" plasmid and followed the dynamics of bacteria over time. Interestingly, previous methods developed to estimate Mtb replication and death rates using similar data for Mtb infection of mice failed to describe our novel data on Mtb dynamics in rabbits; we showed that heterogeneous dynamics of Mtb in semi-independent subpopulations in lungs of Mtb-infected rabbits may be one explanation of this failure of the method. Our results highlight potential differences in Mtb dynamics in different mammalian hosts and suggest ways to evaluate heterogeneity of Mtb replication and death rates in vivo.

Active phase and amplitude temperature control method for cryostat: A case in the thermodynamic temperature international comparison system

Achieving highly stable temperature control is essential for cryostats. However, the cryocooler used in cryostats, such as the pulse tube cryocooler, produces regular sinusoidal temperature fluctuations due to its inherent working principles. To establish a highly stable temperature environment, an active phase and amplitude control method for suppressing temperature fluctuation is proposed in this paper, by which the optimal phase and amplitude can be predicted in theory. To validate this approach, a thermal response model was developed based on experiments conducted in the thermodynamic temperature international comparison cryostat at approximately 3.7 K. Based on this model, we conducted both single-stage and multi-stage active temperature control experiments. The results demonstrate that the temperature control effect is the best when the active temperature control current is added at the second cold head, the fluctuation of the comparison copper block from 3.3mK to 0.166mK with 94.9 % suppression rate. The multi-stage joint temperature control reduced the temperature fluctuation with an attenuation rate of 95.4 %. These findings offer significant potential for improving temperature stability in future thermodynamic temperature comparison experiments.

Engineering Shape to Overcome Contraction: The Role of Polymer-Collagen Hybrids in Advanced Dermal Substitutes.

Collagen gels are the standard dermal equivalents par excellence, however the problem of rapid cell-mediated contraction remains unresolved. Therefore, the development of hybrid constructs (HCs) based on collagen and polymeric scaffolds is proposed to address the mechanical instability that usually limits the formation of new, functional tissue. Equally important, these synthetic structures should be temporary (degradable) while ensuring that cells are well-adapted to the new extracellular environment. In this study, we screened a library of scaffolds made of various polymers, including homopolymers of polycaprolactone (PCL) and poly D,L-lactide (PLA50), their blends (PCL/PLA50), and copolymers (poly(D,L-lactide-co-caprolactone), PCLLA50) to prepare HCs in a layer-by-layer fashion. The properties of polymers and copolymers along with their processability by electrospinning and 3D-printing were evaluated. Then, we assessed the HCs resistance toward cell-mediated contraction as well as the degradation of the polymeric scaffolds. Our results indicate that scaffolds with higher PLA50 content (e.g., PLA50 100%, PCL/PLA50 or PCLLA50, both at 50/50 caprolactone-to-D,L-lactide molar ratio) presented more drawbacks in terms of handleability and processing, while those with greater PCL presence showed structural steadiness and ease to use. All the scaffolds integrated well with the collagen gel to form the corresponding HCs. With few exceptions, the HCs demonstrated good resistance to cell-derived contraction over 3 weeks. Notably, HCs based on PCLLA50 90/10 (both versions, electrospun or 3D-printed) performed best, showing only a 5%-17% area reduction compared to the 93% observed in collagen-only gels. This copolymer displayed hydrolytic degradation depending on its shape, with up to 45% and 65% loss of molecular weight for the electrospun and 3D-printed forms, respectively, correlating with their progressive change in mechanical features. HCs containing PCLLA50 90/10 also exhibited a better fibroblast distribution, enhanced myofibroblastic differentiation, and a three-fold increase in cell proliferation (when the electrospun type was used) compared to collagen controls. These findings were instrumental in selecting a potential HC that might be used for future experiments in vivo.

Strange-metal behavior without fine tuning in PrV2Al20

Strange-metal behavior observed in the praseodymium-based heavy-fermion material PrV2Al20 has been tentatively interpreted in the framework of proximity to a quantum critical point (QCP) associated with quadrupolar ordering. Here, we demonstrate that an alternative, natural explanation exists without invoking a QCP, in terms of the unconventional nature of the quadrupolar Kondo effect taking place in non-Kramers ions. Using a combination of density-functional theory calculations and analytical arguments, we construct a periodic Anderson model with realistic parameters to describe PrV2Al20. We solve the model using dynamical mean-field theory preserving the model symmetries and demonstrate the non-Fermi liquid strange-metal behavior stemming from the two-channel nature of the quadrupolar Kondo effect. Our calculations provide an explanation for the puzzling temperature dependence in the magnetic susceptibility and provide a basis for analyzing future photoemission experiments. Published by the American Physical Society 2024

TSUNAMI Analyses of the Similarity and Applicability of Zero Energy Deuterium 2 Critical Experiments for Reactor Physics Code Benchmarking for a Pressurized Water Reactor Small Modular Reactor Design Concept

Abstract This paper presents the findings of studies conducted at Canadian Nuclear Laboratories (CNL) to support the development of small modular reactor (SMR) designs. The primary focus of this research was to evaluate the suitability of the zero energy deuterium 2 (ZED-2) critical facility in replicating the reactor physics environment for a pressurized water reactor small modular reactor (PWR-SMR) design concept through similarity and nuclear data sensitivity studies, using the TSUNAMI code suite. It was found that previous ZED-2 experiments would be quite promising for application to a PWR-SMR design. Further similarity and sensitivity studies of hypothetical mixed-lattice substitution experiments, where PWR-SMR fuel assemblies were placed into a substitution region of the ZED-2 critical facility demonstrated improved similarity. Subsequent analyses focused on the impacts of dissolved Gadolinium (Gd) and boron (B) neutron absorbers, suggesting the feasibility of using future ZED-2 experiments to more closely replicate PWR-SMR reactor physics behavior. Building on these initial findings, the design for PWR-SMR fuel assembly substitution experiments in the ZED-2 facility were explored further. These hypothetical experiments feature water-cooled PWR-type fuel assemblies inside a shroud, surrounded by heavy-water-moderated CANdu FLEXible, Low Enriched Uranium, Recovered Uranium (CANFLEX-LEU/CANFLEX-RU) fuel channels. Similarity and sensitivity studies indicate a very high level of similarity of these experiments for PWR-SMR design applications.

Su(3) symmetry analysis in charmed baryon two body decays with penguin diagram contribution

An increasing number of experimental measurements from the BESIII, Belle, and Belle-II collaborations encourage investigations into charmed baryon two-body decay processes. By including contributions from the penguin diagrams that are ignored in previous studies, we perform a global analysis with SU(3) flavor symmetry. Assuming all form factors are real, we achieve a remarkable minimal χ2/d.o.f=0.788\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\chi ^{2}/d.o.f = 0.788$$\\end{document} and find that the contribution of the amplitude proportional to Vcb∗Vub\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$V_{cb}^*V_{ub}$$\\end{document} is of the order ∼0.01\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\sim 0.01$$\\end{document}, comparable with the contribution of the tree-level diagram. Additionally, by using the KPW theorem to reduce the number of amplitudes from 13 to 7 in the leading contribution, it becomes possible to consider the complex form factor case for the leading IRA amplitude in the global analysis. However, the analysis of complex form factors significantly conflicts with the experimental data Br(Ξc0→Ξ-π+)\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$Br(\\Xi _c^0\\rightarrow \\Xi ^-\\pi ^+)$$\\end{document}, and by excluding this data, χ2/d.o.f\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\chi ^2/d.o.f$$\\end{document} is reduced from 5.95 to 1.19. Although the analysis of complex form factors shows a significant central value of the penguin diagram contribution, the large errors from the corresponding form factors make it a challenge to precisely determine its true contribution. Consequently, the direct CP violation in decay processes is predicted to be approximately zero. With more data in future experiments, the penguin diagram contribution with the amplitude proportional to Vcb∗Vub\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$V_{cb}^*V_{ub}$$\\end{document} will be precisely determined, allowing for a more accurate prediction of CP violation. Our analysis necessitates further theoretical investigations and experimental measurements in the future.

Angle-insensitive all-optical diodes in a large range of incidence by photonic heterostructure containing hyperbolic metamaterials

We theoretically and systematically investigate angle-insensitive all-optical diodes using photonic heterostructures composed of different one-dimensional photonic crystals containing hyperbolic metamaterials. Hyperbolic metamaterials comprise metals with high nonlinear coefficients and transparent dielectrics. The results indicate that the photonic heterostructure shows angle insensitivity in transmittance, transmission contrast, and the critical intensity of the threshold over a large range of incidence, achieving a high transmission contrast of approximately 0.93 between forward and backward incident light. This angle insensitivity originates from the phase-variation compensation effect in a basic unit composed of hyperbolic metamaterials and transparent dielectric materials under TM polarization. These results provide important reference values for future experiments.

8743 Regulation Of Tryptophan 2,3-Dioxygenase 2 And Androgen Receptor In Triple-Negative Breast Cancer

Abstract Disclosure: L. Kuo: None. K. Lacey: None. N. Spoelstra: None. J.K. Richer: None. Background: Triple Negative Breast Cancer (TNBC) is an aggressive subtype with a peak recurrence rate within 3-5 years after initial diagnosis. Androgen receptor (AR) and Tryptophan 2,3-dioxygenase 2 (TDO2) are elevated in TNBC cells grown under anchorage-independent conditions and facilitate anoikis resistance. Several studies reported that the luminal AR subset of TNBC showed a positive enrichment of the tryptophan (TRP) catabolism pathway gene signature. Recent studies have demonstrated that aryl hydrocarbon receptor (AhR) inhibition decreases AR promoter transcriptional activity in prostate cancer. We have observed that TNBC with high AR have high levels of genes/proteins in the tryptophan catabolism pathway including the rate limiting enzyme TDO2. Hypothesis: TDO2 activity regulates AR expression through the downstream tryptophan catabolite kynurenine (KYN), which binds and activates AhR and AhR and AR may work together to promote TNBC progression. Methods: Human TNBC cell lines: MDA-MB-453, BT549, SUM159PT ​ were used in this study and the expression of TDO2 and AR were examined by immunohistochemistry (IHC) and western blot. TDO2 was stably knocked down (KD) via shRNA or overexpressed (OE) using lentivirus transduction. Mass spectrometry-based metabolomics were used to measure changes in TRP catabolite levels, and a cytokine array (R&D) was utilized to analyze conditioned media. Results: TDO2 and AR protein are positively correlated in human TNBC cell lines. Although overexpression of TDO2 did not alter AR protein expression in SUM159PT, in the human MDA-MB-453, which have high levels of AR, knockdown of TDO2 (shTDO2) reduced AR protein by 40% and production of kynurenine (KYN) was reduced by 50% (p<0.0001). This suggests that TDO2 activity, specifically KYN binding to AhR, may influence AR protein levels. Conditioned media (CM) from TDO2 OE and KD respectively increased and decreased CXCL5 and IGFBP2. Lastly, TCGA database analysis of TNBC tumors with high levels of both AhR and AR showed significantly shorter patient survival (HR=1.47, log rank p=0.046) compared to those with tumors with lower amounts of both genes. Conclusion: TDO2 and AR are positively correlated in human TNBC cell lines. Tumors with high levels of both AR and AhR result in significantly shorter survival time for TNBC patients. Knockdown of TDO2 significantly reduced KYN production and AR expression in luminal AR TNBC cell lines and future experiments will focus on the underlying mechanism behind this and whether it is dependent on KYN-mediated activation of AhR and if AhR stabilizes AR in TNBC. Presentation: 6/3/2024

8743 Regulation of Tryptophan 2,3-Dioxygenase 2 and Androgen Receptor in Triple-Negative Breast Cancer

Abstract Disclosure: L. Kuo: None. K. Lacey: None. N. Spoelstra: None. J.K. Richer: None. Background: Triple Negative Breast Cancer (TNBC) is an aggressive subtype with a peak recurrence rate within 3-5 years after initial diagnosis. Androgen receptor (AR) and Tryptophan 2,3-dioxygenase 2 (TDO2) are elevated in TNBC cells grown under anchorage-independent conditions and facilitate anoikis resistance. Several studies reported that the luminal AR subset of TNBC showed a positive enrichment of the tryptophan (TRP) catabolism pathway gene signature. Recent studies have demonstrated that aryl hydrocarbon receptor (AhR) inhibition decreases AR promoter transcriptional activity in prostate cancer. We have observed that TNBC with high AR have high levels of genes/proteins in the tryptophan catabolism pathway including the rate limiting enzyme TDO2. Hypothesis: TDO2 activity regulates AR expression through the downstream tryptophan catabolite kynurenine (KYN), which binds and activates AhR and AhR and AR may work together to promote TNBC progression. Methods: Human TNBC cell lines: MDA-MB-453, BT549, SUM159PT ​ were used in this study and the expression of TDO2 and AR were examined by immunohistochemistry (IHC) and western blot. TDO2 was stably knocked down (KD) via shRNA or overexpressed (OE) using lentivirus transduction. Mass spectrometry-based metabolomics were used to measure changes in TRP catabolite levels, and a cytokine array (R&D) was utilized to analyze conditioned media. Results: TDO2 and AR protein are positively correlated in human TNBC cell lines. Although overexpression of TDO2 did not alter AR protein expression in SUM159PT, in the human MDA-MB-453, which have high levels of AR, knockdown of TDO2 (shTDO2) reduced AR protein by 40% and production of kynurenine (KYN) was reduced by 50% (p<0.0001). This suggests that TDO2 activity, specifically KYN binding to AhR, may influence AR protein levels. Conditioned media (CM) from TDO2 OE and KD respectively increased and decreased CXCL5 and IGFBP2. Lastly, TCGA database analysis of TNBC tumors with high levels of both AhR and AR showed significantly shorter patient survival (HR=1.47, log rank p=0.046) compared to those with tumors with lower amounts of both genes. Conclusion: TDO2 and AR are positively correlated in human TNBC cell lines. Tumors with high levels of both AR and AhR result in significantly shorter survival time for TNBC patients. Knockdown of TDO2 significantly reduced KYN production and AR expression in luminal AR TNBC cell lines and future experiments will focus on the underlying mechanism behind this and whether it is dependent on KYN-mediated activation of AhR and if AhR stabilizes AR in TNBC. Presentation: 6/3/2024

4D Track Reconstruction on Free-Streaming Data with PANDA at FAIR

A new generation of accelerator-based experiments in subatomic physics is being developed, where the challenge of separating rare signal processes from background at high intensities requires a change of trigger paradigm. At the future PANDA experiment at FAIR, hardware triggers will be abandoned and instead a purely software-based system will be used. This requires novel reconstruction methods with the ability to process data from many beam–target interactions simultaneously. A 4D tracking algorithm, based on the cellular automaton, has been developed which will utilize the timing information from detector signals. Simulation studies have been performed to test its performance on the foreseen free-streaming, i.e., continuous readout from the PANDA detector, where all processing is on the software level. For this purpose, a novel quality assurance method, tailored for tracking on free-streaming data has been developed and implemented. Benchmark studies on PANDA software show that at higher interaction rates, 4D tracking performs better than the 3D algorithm in terms of efficiency, 84%\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\%$$\\end{document} compared to 77%\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\%$$\\end{document}. In addition, the fake track suppression is greatly improved, reducing the rate of fake tracks of “ghosts” by about 50%\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\%$$\\end{document}.

Road-crossing behavior and safety of pedestrians facing autonomous vehicles with an acceleration indicator eHMI in VR traffic flow

To support pedestrian road-crossing behavior in the presence of autonomous vehicles (AVs), we propose a novel external Human-Machine Interface (eHMI) that enhances the vehicle’s inherent motion cues, which pedestrians already use in traditional traffic. Based on the Beta Movement, the eHMI design consists of light bands with multiple arrows moving forward or backward to indicate the vehicle’s accelerating or decelerating motion state. We measured pedestrians’ behavior and perceived safety when faced with AVs equipped with this acceleration indicator eHMI in a virtual reality (VR) street scene, where pedestrians had road-crossing tasks and vehicles did not always yield. We found that pedestrians exhibited crossing behavior according to the motion state of the oncoming vehicle. They almost always crossed if the vehicle yielded but considered the gap size to cross when it did not. With the acceleration indicator eHMI, they crossed more frequently if the vehicle yielded, yet less frequently if it did not. Although risky crossings could not be fully prevented, pedestrians crossed faster and maintained larger safety margins when the non-yielding vehicle used this eHMI. Additionally, pedestrians started to cross earlier when the eHMI indicated the vehicle’s decelerating state. Regarding pedestrians’ perceived safety, the eHMI did not increase perceived safety for decelerating vehicles but effectively decreased the perceived safety for accelerating vehicles. These results demonstrate the potential of enhancing vehicles’ inherent motion cues with eHMIs, but also imply that making vehicles’ motion state more apparent might not simply increase pedestrians’ perceived safety. Overall, a trade-off seems to exist that it is pedestrians’ sense of unsafety that leads to their safe road-crossing behavior. Suggestions for future research and VR experiments were also discussed.

A method for studying the effect of diegetic vs. non-diegetic rating interfaces on the assessment of virtual acoustic environments

Auditory perception experiments conducted in VR come along with a high degree of ecological validity while maintaining a controlled laboratory setting. However, since the participant's vision is occupied during simulation, conventional evaluation instruments like rating scales need to be relocated to the virtual environment to allow for immediate stimulus rating. This can be achieved either by the use of diegetic interfaces, which are implied to be a part of the simulation (e.g., a screen held by the participant's avatar) or non-diegetic interfaces, which do not claim to originate from the simulated reality (e.g., sliders and text elements laid on top of the participant's view). It is unknown whether the choice of interface does affect the participant's perception of the virtual environment - including its acoustic components. To address this shortcoming, a methodological study is presented. Participants are exposed to a virtual scenario simulating an urban green area with nearby ambience sound sources. In this setting, they are provided with diegetic and non-diegetic rating interfaces to assess the acoustic environment. Subsequently, perceived presence and involvement in the virtual environment are surveyed as well. This way, the study provides useful guidelines for future VR experiments assessing soundscape-related aspects of perception.