Future Breeding Programs Research Articles

Salinity Tolerance of Diplotaxis tenuifolia Varieties Growing in Spring–Summer Season Under Mediterranean Greenhouse and Optimal Growing Conditions

Salinity is one of the principal abiotic stresses that occurs in the Mediterranean area, causing loss of productivity and decrease of vegetable crop quality. The effect of salinity (0, 25, 75, 150 mM NaCl) was evaluated in three Diplotaxis tenuifolia varieties (Dragon Tongue, Capriccio, Piccante), previously selected for salinity tolerance and high glucosinolates production in leaves. The aim of this research was to explore the salinity tolerance of three wild rocket varieties cultivated under optimal temperature conditions and under high temperature that typically characterized the Mediterranean greenhouse. Biometric, biomass, pigment production and physiological parameters were evaluated. Biometric, physiological, and biochemical parameters significantly varied because of variety, salt level used and environmental conditions. PCA analysis highlighted that the two cultivation systems deeply affected the wild rockets response to salt stress. In general, under optimal growing conditions, wild rocket varieties showed higher growth parameters compared to greenhouse conditions. Overall Capriccio was the most susceptible variety to salinity, while Dragon Tongue (V1) and Piccante (V3) were more tolerant to salt stress. Furthermore, in both growing conditions V1 was the less productive variety while V3 showed an opposite trend. Interestingly, gene (DtOxo and DtGst) expression analysis revealed a significant increase of the target gene expression as response of salinity levels, with a clear increase of DtOxo level in V1 and V3. The results obtained in this study can be useful to plan future breeding programs aimed to increase rocket quality grown under Mediterranean conditions.

Leaf phenotypic plasticity of European ash (Fraxinus excelsior) at its northern range in Dinaric Alps

The Dinaric Alps have been recognised on numerous occasions as a biodiversity hotspot. They host a variety of species with great importance in sustainable forestry operations and nature conservation. One such species is the European ash (Fraxinus excelsior L., Oleaceae), a broad-leaved, wind-pollinated and wind-dispersed forest tree. In this paper, we aimed to determine the morphological variability of the European ash populations of the northern Dinaric Alps. For this purpose, leaf samples from 10 individuals in seven populations were collected. Morphometric analysis of intra- and interpopulation variability was conducted using 19 morphological leaf traits. We determined great variability of trees within, and small variability among, populations. The variables that best discriminated studied populations were those relating to leaflet shape. Based on these variables, populations were grouped into two clusters. The first cluster encompassed individuals with acute leaflets, found in drier and rockier habitats, whereas the second cluster, defined by more rounded leaflets, was found in mesophilous and nutrient-rich habitats. However, this research revealed no influence of geographical or bioclimatic distances on morphological variability, which indicates that the rockiness and soil are most likely two predominant factors in shaping the phenotypic plasticity of European ash populations. These results are of great significance in the planning of future forest breeding programs, as populations from drier habitats are likely to persist and spread due to their adaptation to water scarcity, which will become more pronounced in the future.

Evaluation of the Performance of Okra (Abelmoschus esculentus l. Moench) Genotypes for Growth, Yield, and Quality Parameters

The present investigation was carried out to evaluate the performance of okra genotypes for growth, yield and quality parameters. Okra is often cross-pollinated and is grown primarily for its fruits, which have various culinary uses. Tender fruits are commonly boiled or used in sliced and fried dishes. Okra fruits are also dried in the sun, canned, or dehydrated for preservation during the off-season. The study was carried out at New Orchard, Main Agricultural Research Station, UAS, Raichur, during late Kharif 2021-22 using thirty-one genotypes (including the check variety Arka Anamika) and laid out in Randomized Block Design (RBD) with three replications. The analysis of variance revealed significant differences (p < 0.05) among genotypes for all traits studied. Genotype OPL-2127 recorded the highest plant height (96.52 cm), stem girth (14.26 mm), number of leaves per plant (23.20), leaf area index (3.08), number of nodes per plant (13.60), chlorophyll content (59.61 SPAD readings), fruit length (18.69 cm), fruit yield per plant (293.53 g), fruit yield per hectare (16.4 t) and it is found to be superior over the other okra genotypes under the study. The highest fruit girth (41.36 mm) was noted in the genotypes OPL-2108. The genotype OPL-2119 recorded the minimum number of days to 50 per cent flowering (38.00) which indicates the earliness of the genotypes. OPL-2023 recorded the highest fibre content (16.09 %). The genotypes OPL-2127, OPL-2121, OPL-2116, OPL-2115 and OPL-2113 were found to be superior okra genotypes for yield and quality and they can be used in future breeding and crop improvement program.

Genome-wide identified VvOFP genes family and VvOFP4 functional characterization provide insight into fruit shape in grape

Ovate Family Proteins (OFPs) are emerging as novel transcriptional regulators of fruit shape. Despite their established role in various species, their involvement in regulating grape fruit shape remains understudied. This study encompassed a comprehensive evaluation of 16 grape OFP genes in total at the whole genome level. Phylogenetic and synteny analyses established a close relationship between grape VvOFP genes and their tomato counterparts. Expression profiling post-treatment with gibberellic acid (GA3) and thidiazuron (TDZ) revealed that certain OFP genes responded to these regulators, with VvOFP4 showing peak expression three days post-anthesis. Functional assays via overexpression of VvOFP4 in tobacco and tomato altered the morphology of both vegetative and reproductive organs, including leaves, stamens, and fruits/pods. Paraffin sections of transgenic tobacco stems and tomato fruits demonstrated that VvOFP4 overexpression modifies cell dimensions, leading to changes in organ morphology. Additionally, treatments with GA3 and TDZ similarly influenced the shape of grape pulp cells and thereby the overall fruit morphology. These findings suggest that the VvOFP4 gene plays a crucial role in fruit shape determination by modulating cell shape and presents a potential target for future grape breeding programs aimed at diversifying fruit shapes.

Impact of Fusarium spp. on different maize commercial hybrids: disease evaluation and mycotoxin contamination

Maize is one of the most important crops cultivated worldwide, whose production can be affected by the presence of several pathogens. Fusarium verticillioides and Fusarium graminearum are the most predominant pathogens affecting maize ears. However, few studies have been focused on studying the interaction between both pathogens in field conditions. For this reason, the aim of the present work was to evaluate the interaction between F. graminearum and F. verticillioides in different genotypes of maize under field conditions. Field experiments were carried out during two growing seasons in Azul, Argentina, including 12 commercial hybrids of maize, which were inoculated with F. graminearum, F. verticillioides, and a mixture of both pathogens. Phenotypic traits (plant height, plant diameter, tiller and cob number, and radiation interception), disease evaluation, and mycotoxin contamination were analyzed. The results showed significant differences between genotypes in disease severity (DS) for both years. In general terms, higher values of DS were reported in 2020 (21.70% ± 0.40) than in 2021 (16.50% ± 0.20). Different climatic conditions registered along the assay, especially precipitations and relative humidity, could be responsible for the differences observed over the years. Moreover, no significant correlations were found regarding DS and mycotoxin contamination for each genotype. For these reasons, an automatic correspondence between DS and mycotoxin contamination could lead to wrong agronomic decisions. The present study points out novel information regarding plant–pathogen interaction (maize-F. verticillioides/F. graminearum) under field conditions that could be useful for future maize breeding programmes.

Exploring population genetics for mutant mungbean [Vigna radiata (L.) R. Wilczek] lines: Insights from augmented block design

AbstractThe current study was initiated to assess mungbean [Vigna radiata (L.) R. Wilczek] mutant lines for yield and their attributes in the mutation generation third (M3) generation using an augmented block design. Note that 1200 mutated mungbean lines were selected from the γ‐irradiated (400–700 Gy) population and distributed into 21 blocks, along with three popular varieties (checks) replicated in each block. To evaluate mean performance, variance, and population genetics, the observations on days to flowering, days to maturity, plant height (cm), number of clusters per plant, number of pods per plant, pod length (cm), protein percentage, seed index, and seed yield (g) were measured and analyzed. All the lines under consideration showed highly significant variations for all the variables, except for days to flowering. Seventy‐nine mutant lines were found to have significantly better yield attributes than checks and are currently being evaluated under station trial. Skewness and kurtosis analysis unveiled the presence of gene interactions, offering opportunities for targeted improvement and efficacy of γ rays as a mutagen, facilitating the release of variability within the population. Future mungbean breeding programs will benefit from the successful isolation of mutant plants with yield‐enhancing traits, such as up to 20 clusters per plant, 71 pods per plant, 8.55 cm pod length, 26.69% protein content (5% higher than parent), and 29 gram seeds per plant. In early selection cycles without the need for replicated trials, this study demonstrated the effectiveness of the mutagen and augmented design in creating novel variations, evaluating and identifying superior genotypes with improved yield potential.

Morphological characterization of shallot (Allium cepa L. var. aggregatum) segregating populations obtained from natural-outcrossing in Ethiopial-outcrossing in Ethiopia

Shallot is a vegetable and condiment crop widely used in Ethiopia and globally. However, absence of improved and adaptable varieties has been the major cause of low productivity. Narrow genetic base of local shallot germplasm owing to vegetative reproduction of the crop, among others, has been the root cause of low productivity. Nevertheless, some plants within the germplasm were observed bolting and producing viable seeds, presenting an opportunity for genetic diversification. Consequently, a germplasm enhancement program was initiated using these naturally outcrossing genotypes where about eighty-one genotypes were generated. The present study was thus undertaken with the objective of characterizing, classifying, and selecting the eighty-one genotypes for future breeding activities. The genotypes were planted in 9x9 simple lattice design with two replications at Debre Zeit Agricultural Research Center (Ethiopia) during the dry (irrigated) season of 2021. The genotypes were evaluated for fifteen growth, yield, and quality traits. Significant variations were observed among the genotypes in terms of bulb yield, bulb height and diameter, total soluble solids, bolting percentage, and bulb skin color. Bulb yield of the genotypes ranged from 31.33 t/ha in DZSHT-79-1A to 9.63 t//ha in DZSHT-45-1A-1. DZSHT-51-2 (207.93 g) was the highest yielder per plant whereas DZSHT-065-6/90 (74.51 g) was the lowest yielder. DZSHT-14-2-1/90 had the thickest bulb (44.69 mm) significantly thicker than twenty two genotypes which had bulb diameter ranging from 28.92 mm to 20.29 mm. DZSHT-81-1/90 was a genotype with the longest bulb height (52.33 mm) while DZSHT-147-1C was a genotype with the shortest bulb (33.12 mm). DZSHT-307-1/90 had the highest TSS (16.78°Brix) significantly differing from DZSHT-002/07 which had the lowest TSS (11.17 °Brix). Dry matter of the genotypes ranged from 12.00% to 22.79%. DZSHT-004/07, DZSHT-111-2-1, DZSHT-41-2B and DZSHT-72-2 had DM% greater than 20% which coupled with greater than 14 °Brix could make them suitable for dehydrated shallots. Among the 81 genotypes characterized 4 (4.9%), 7 (8.6%), 13 (16.1%), 28 (34.6%) and 29 (35.8%) were yellow, golden, light red, red and dark red in colour, respectively. Fifteen of the genotypes had at least 50% bolting plants whereas twenty nine of the genotypes had less than 25% bolting. The results revealed that seven principal components explained approximately 76% of the observed variation. Cluster analysis grouped the genotypes into seven clusters, with the majority falling into three clusters. The study successfully identified genotypes with diverse and important traits and availed both the genotypes and the information for future breeding programs. These genotypes could be used for the development of improved hybrid and open pollinated shallot varieties with higher yield, quality and pest resistance/tolerance attributes.

Comprehensive analysis of drought tolerance in pure lines derived from half-diallel crosses of upland cotton (Gossypium hirsutum L.)

Drought has a significant impact on plants, affecting their growth, development and survival. This study focuses on evaluating the impact of drought stress, a significant abiotic factor, on the agronomic and fiber parameters of potential cotton (Gossypium hirsutum L.) lines with the aim of developing drought-tolerant varieties. The experiment involved two irrigation regimes—well-watered (100% field capacity) and deficit irrigation (50% field capacity)—conducted on F9–F10 generations. Key fiber parameters, including fiber length, boll weight, fiber strength, and lint percentage, were identified as crucial selection criteria under both well-watered and deficit irrigation conditions. Notably, boll number emerged as the decisive parameter in both F9 and F10 generations. The study employed univariate and multivariate analyses, such as PCA, heat map cluster, correlation analysis, and decision tree, which consistently highlighted fiber length, boll weight, fiber strength and lint (ginning) percentage the key factor. In the F10 generation, the integration of decision tree and heat map cluster results led to the identification of 8 promising lines. These selected genotypes have potential for inclusion in future cotton breeding programmes, offering the opportunity to increase drought tolerance and improve cotton yield and productivity. Their resilience to environmental stresses makes them promising candidates for improving overall cotton performance under challenging conditions.

Unveiling Genotypic Response of Chickpea to Moisture Stress Based on Morpho‐Physiological Parameters in the Eastern Indo‐Gangetic Plains

ABSTRACTIn the eastern Indo‐Gangetic plains, chickpea is grown postrice cultivation mostly under rainfed condition with residual soil moisture which adversely affects branching as well as pod and seed development, ultimately resulting in substantial yield losses. The current study analysed the moisture stress response of 12 chickpea genotypes with control for different morpho‐physiological traits in two sets of field experiments carried out during the year 2017–18 and 2018–19. The current study observed varying response of chickpea genotypes under moisture stress condition with average yield reduction from 11.79% to 24.77%. Mean yield of genotypes under stress condition showed a strong positive association with yield index (1.00**) and stress tolerance index (0.915**). The biplot principal component analysis revealed maximum potential of three chickpea genotypes (DBGC 1, Pusa 256 and DBGC 2) for grain yield and biological yield under moisture stress condition. The correlation analysis showed a significant association of yield with physiological parameters such as photosynthetic rate (0.363**), stomatal conductance (0.364**) and transpiration rate (0.292*). The three higher yielding genotypes relatively maintained biological yield, yield plant−1, 100 seed weight and photosynthesis rate and showed reduced rates of stomatal conductance and transpiration under moisture stress condition. The study found variable genotypic response to moisture stress and showed that yield index as well as stress tolerance index was more effective to identify superior genotypes for moisture stress condition. The superior genotypes identified in the present study may be considered for rainfed areas of eastern Indo‐Gangetic plains and can be used in future chickpea breeding programs for drought tolerance.

Morpho-physiological characterization and selection of heat tolerant wheat lines using selection indices

Heat stress is one of the major constraints in wheat production due to its adverse effect on grain yield and component traits. Moreover, it negatively affects the physiological, biochemical and quality traits of wheat. Therefore, selection of heat tolerant wheat lines is one of the major breeding goals for wheat scientist. In present study, 238 recombinant inbred lines were evaluated along with their parents WH 711 × WH 542 under timely and late sown (heat stress) condition. The heat tolerance indices were calculated based on grain yield under normal and stress conditions. The mean grain yield of 238 wheat lines was reduced by 20.54% suggesting critical effect of heat stress on grain yield. The harmonic mean (HM), stress tolerance index (STI), mean productivity (MP), geometric mean productivity (GMP), and mean relative performance (MRP) were correlated significantly and positively with grain yield under stress and normal conditions. Whereas, Tolerance index (TOL) was correlated negatively with grain yield under stress conditions and positively under normal condition. Based on STI, MP, HM, GMP and MRP, wheat lines WL 92, WL 119, WL 114, WL 110, WL 6 were identified as heat tolerant and could be utilized as potential lines for increasing heat stress tolerance of future wheat breeding programs.

Unravelling the biochemical traits of traditional rice landraces of Kerala

Biochemical characterisatics of 70 traditional landraces of rice (Oryza sativa L.) of Kerala including various micronutrients, rice bran oil and oryzanol content were analyzed. Traditional varieties have demonstrated their potential as valuable reservoirs of nutrients, indicating their suitability for incorporation into future breeding programmes aimed to develop nutritionally enriched rice varieties. Among the genotypes studied Oorkayama and Thekkan were identified with highest rice bran oil content and γ oryzanol content respectively. Fe, Zn, Na, K, carotene and anthocyanin content showed a wide range of variation among the genotypes. The GCV estimates were high for rice bran oil content and oryzanol content indicating higher magnitude of variability due to genotypes for these characters. The investigation suggests preponderance of additive gene effects for all the biochemical constituents under study as indicated by the high heritability coupled with high genetic advance.

Study on Genetic Variability for Forage Yield and Quality Traits in Forage Sorghum [Sorghum bicolor L. Moench

The initial research related to germplasm screening was carried out in the experimental area of Instructional Dairy Farm, GBPUAT Pantnagar during kharif season 2018. The experimental material for this experiment consisted of two hundreds and eighty diverse germplasm lines of sorghum along with six checks viz., SSG 59-3, Pant Chari- 5, Pant Chari- 6, CSV-21 F, CSH-22S, and CSV-24SS. The germplasm lines were evaluated in Augmented Block Design for nineteen forage yield related traits and thirteen quality traits. The statistical analysis for genetic variability was done using analysis of variance (ANOVA), heritability (h2), genotypic coefficient of variation (GCV), phenotypic coefficient of variation (PCV), and genetic advance (GA). The analysis of variance revealed that the genotypes and checks were showing a highly significant differences for all the traits under study indicated existence of inherent genetic differences among genotypes for different characters. Mean performance of forage yield and quality related traits exhibited a wide range of variability. GCV and PCV values were observed low for most of the characters whereas high for anthracnose, zonate leaf spot and shoot fly incidence. The value of genetic advance was observed high for leaf area, green fodder yield per plant, dry fodder yield per plant, hydrocyanic acid content, plant height, anthracnose, and shoot fly incidence were relatively more than other characters. Genetic advance as per cent of mean was observed low for days to maturity, dry matter per cent, in-vitro dry matter disappearance, neutral detergent fiber, acid detergent fiber, cellulose content whereas high for leaf area, flag leaf width, panicle length, panicle width, 1000-grains weight, grain yield per plant, green fodder yield per plant, dry fodder yield per plant, total soluble solids, silica content, anthracnose, zonate leaf spot and shoot fly incidence. Heritability values were found high for all the traits under study. The genotypes bearing the desired values for different genetic variability parameters and mean performance for can be exploited in future breeding programmes for the improving forage genotypes. These genotypes can be used as donor parents in crop improvement programme for improving forage yield and quality related traits along with improved resistance against anthracnose, shooty fly and zonate leaf spot.

Morphological Characterization and Genetic Diversity Analyses of Yield and Yield Attributing Traits in Sweet Corn (Zea mays var. saccharata)

The genetic diversity among the genotypes were useful resources that enabled breeders to comprehend the performance of attributes or genotypes. This understanding is crucial in selecting appropriate genotypes or traits for hybridization programmes. A total of 15 sweet corn genotypes were utilized to assess the characteristics of genetic diversity, correlation, path coefficient and genetic divergence study. The phenotypic coefficient of variation (PCV) exceeded the genotypic coefficient of variation (GCV) for all the traits examined, suggesting certain degree of interaction with the environment. The heritability estimates for all variables examined in this study were found to be high. The genetic advance as per cent of mean (GAM) identified a significant difference between high and low values. The traits ear weight with husk, ear weight without husk, husk ratio, chlorophyll content, ear length, ear girth, number of rows per ear, number of kernels per row, 100 kernel weight, shelling percentage, cob yield per plot and fodder yield per plot exhibited high heritability and high GAM. Furthermore, the correlation analysis revealed a significant and positive association for the aforementioned attributes. Additionally, the path coefficient analysis for these traits indicated a favourable direct influence. A total of 15 genotypes were categorized into seven clusters based on their genetic distance. The features that made the biggest contribution to divergence were fodder yield per plot (21%) and shelling percentage (21%), followed by ear weight (14%) and cob yield per plot (10%). The results of this study would be valuable for identifying the desirable characteristics and genetic makeup that can be used in future breeding programmes aimed at enhancing the production of corn cobs in sweet corn.

Metabolomics and transcriptomics analysis revealed theresponse mechanism of alfalfa to combined cold and saline-alkali stress.

Cold and saline-alkali stress are frequently encountered by plants, and they often occur simultaneously in saline-alkali soils at mid to high latitudes, constraining forage crop distribution and production. However, the mechanisms by which forage crops respond to the combination of cold and saline-alkali stress remain unknown. Alfalfa (Medicago sativa L.) is one of the most essential forage grasses in the world. In this study, we analyzed the complex response mechanisms of two alfalfa species (Zhaodong [ZD] and Blue Moon [BM]) to combined cold and saline-alkali stress using multi-omics. The results revealed that ZD had a greater ability to tolerate combined stress than BM. The tricarboxylic acid cycles of the two varieties responded positively to the combined stress, with ZD accumulating more sugars, amino acids, and jasmonic acid. The gene expression and flavonoid content of the flavonoid biosynthesis pathway were significantly different between the two varieties. Weighted gene co-expression network analysis and co-expression network analysis based on RNA-Seq data suggested that the MsMYB12 gene may respond to combined stress by regulating the flavonoid biosynthesis pathway. MsMYB12 can directly bind to the promoter of MsFLS13 and promote its expression. Moreover, MsFLS13 overexpression can enhance flavonol accumulation and antioxidant capacity, which can improve combined stress tolerance. These findings provide new insights into improving alfalfa resistance to combined cold and saline-alkali stress, showing that flavonoids are essential for plant resistance to combined stresses, and provide theoretical guidance for future breeding programs.

Identification of anther thermotolerance genes by the integration of linkage and association analysis in maize.

Maize is one of the world's most important staple crops, yet its production is increasingly threatened by the rising frequency of high-temperature stress (HTS). To investigate the genetic basis of anther thermotolerance under field conditions, we performed linkage and association analysis to identify HTS response quantitative trait loci (QTL) using three recombinant inbred line (RIL) populations and an association panel containing 375 diverse maize inbred lines. These analyses resulted in the identification of 16 co-located large QTL intervals. Among the 37 candidate genes identified in these QTL intervals, five have rice or Arabidopsis homologs known to influence pollen and filament development. Notably, one of the candidate genes, ZmDUP707, has been subject to selection pressure during breeding. Its expression is suppressed by HTS, leading to pollen abortion and barren seeds. We also identified several additional candidate genes potentially underly QTL previously reported by other researchers. Taken together, our results provide a pool of valuable candidate genes that could be employed by future breeding programs aiming at enhancing maize HTS tolerance.

Flower color modification in Torenia fournieri by genetic engineering of betacyanin pigments

BackgroundBetalains are reddish and yellow pigments that accumulate in a few plant species of the order Caryophyllales. These pigments have antioxidant and medicinal properties and can be used as functional foods. They also enhance resistance to stress or disease in crops. Several plant species belonging to other orders have been genetically engineered to express betalain pigments. Betalains can also be used for flower color modification in ornamental plants, as they confer vivid colors, like red and yellow. To date, betalain engineering to modify the color of Torenia fournieri—or wishbone flower—a popular ornamental plant, has not been attempted.ResultsWe report the production of purple-reddish-flowered torenia plants from the purple torenia cultivar “Crown Violet.” Three betalain-biosynthetic genes encoding CYP76AD1, dihydroxyphenylalanine (DOPA) 4,5-dioxygenase (DOD), and cyclo-DOPA 5-O-glucosyltransferase (5GT) were constitutively ectopically expressed under the cauliflower mosaic virus (CaMV) 35S promoter, and their expression was confirmed by quantitative real-time PCR (qRT-PCR) analysis. The color traits, measured by spectrophotometric colorimeter and spectral absorbance of fresh petal extracts, revealed a successful flower color modification from purple to reddish. Red pigmentation was also observed in whole plants. LC-DAD-MS and HPLC analyses confirmed that the additional accumulated pigments were betacyanins—mainly betanin (betanidin 5-O-glucoside) and, to a lesser extent, isobetanin (isobetanidin 5-O-glucoside). The five endogenous anthocyanins in torenia flower petals were also detected.ConclusionsThis study demonstrates the possibility of foreign betacyanin accumulation in addition to native pigments in torenia, a popular garden bedding plant. To our knowledge, this is the first report presenting engineered expression of betalain pigments in the family Linderniaceae. Genetic engineering of betalains would be valuable in increasing the flower color variation in future breeding programs for torenia.

Morphological evaluation and yield performance of lentil genotypes against stemphylium blight

Experiment was designed to screen out lentil genotypes against Stemphylium blight (SB). Thirty-five test genotypes along with one moderately susceptible popular local control ‘Moitree’ were grown under natural epiphytotic condition in the farm of BCKV during 2020-21 and 2021-22. Genotypes wise crop phenological characters, yield and disease severity data were recorded. The result revealed, genotypes ILL10838/ILWL11/X2016S was resistant to SB with highest yield (1184.00 kg/ha) followed by 8114/10956/16-1 (1112.20 kg/ha) and IPL 220 (1072.90 kg/ha) with MR reaction. Pooled analysis of two years experimental data used to develop the model Y = 336.21 + 6.12x [R2 = 0.86] explained that there is an increase of yield 6.12 kg/ha with every 1 unit increase in the number of pods per plant. Correlation of determination value (R2 = 0.86) proved the feasibility of the model. Lentil genotypes ILL10838/ ILWL11/X2016S and 8114/10956/16-1 are the best suited for its cultivation and may scaled up for future breeding programme. Bangladesh J. Bot. 53(2): 381-389, 2024 (June)

Determination of genomic regions associated with early storage root formation and bulking in cassava.

Early cassava storage root formation and bulking is a medium of escape that farmers and processors tend to adopt in cases of abiotic and biotic stresses like drought, flood, and destruction by domestic animals. In this study, 220 cassava genotypes from the International Institute of Tropical Agriculture (IITA), National Root Crops Research Institute (NRCRI), International Center for Tropical Agriculture (CIAT), local farmers (from farmer's field), and NextGen project were evaluated in three locations (Umudike, Benue, and Ikenne). The trials were laid out using a split plot in a randomized incomplete block design (alpha lattice) with two replications in 2 years. The storage roots for each plant genotype were sampled or harvested at 3, 6, 9, and 12 month after planting (MAP). All data collected were analyzed using the R-statistical package. The result showed moderate to high heritability among the traits, and there were significant differences (p< 0.05) among the performances of the genotypes. The genome-wide association mapping using the BLINK model detected 45 single-nucleotide polymorphism (SNP) markers significantly associated with the four early storage root bulking and formation traits on Chromosomes 1, 2, 3, 4, 5, 6, 8, 9, 10, 13, 14, 17, and 18.A total of 199 putative candidate genes were found to be directly linked to early storage root bulking and formation. The functions of these candidate genes were further characterized to regulate i) phytohormone biosynthesis, ii) cellular growth and development, and iii) biosynthesis of secondary metabolites for accumulation of starch and defense. Genome-wide association study (GWAS) also revealed the presence of four pleiotropic SNPs, which control starch content, dry matter content, dry yield, and bulking and formation index. The information on the GWAS could be used to develop improved cassava cultivars by breeders. Five genotypes (W940006, NR090146, TMS982123, TMS13F1060P0014, and NR010161) were selected as the best early storage root bulking and formation genotypes across the plant age. These selected cultivars should be used as sources of early storage root bulking and formation in future breeding programs.

Assessment of sex, nut weight, germination vigour, stem growth, and development traits of pistachio cultivars (Pistacia vera L.) used as rootstock

ABSTRACT Pistacia vera L. is the only edible plant with economic importance within Pistacia genus. In the current study, rootstock traits such as growth and development of major pistachio cultivars were evaluated in plants used as rootstock for pistachios in Turkey. The sex of 1008 individuals was determined using a molecular sex marker with HRM analysis. From the eight cultivars, the number of female and male plants were 453 and 413, respectively; and then used for phenotypic measurements. For each measurement period, analysis of variance established that there were no significant ‘cultivar x sex’ interactions or sex effects for stem diameter or stem height. However, the differences among cultivars were highly significant (P < 0.0001) at all measurement times. The maximum stem length and width values were in the progeny of the Ohadi cultivar, with the lowest values recorded for progeny of the Uzun and Kırmızı cultivars. The nut weights and stem properties of the plants were correlated with high positive coefficients, although the germination vigour was not statistically correlated with any other trait. The results of the current paper will aid the selection of candidates for future pistachio rootstock breeding programmes.

Development of a germplasm master set covering variability of high molecular weight glutenin subunits for the GLU-A1 locus in Triticum sp.

Gluten quality is an important characteristic of wheat. Gluten is a viscoelastic protein network that is formed after hydrating and mixing wheat flour. This protein network is mainly composed of glutenins (High and Low Molecular Weight Glutenins (HMW-GS and LMW-GS, respectively)) and gliadins. HMW-GS are codified by the loci GLU-A1, GLU-B1 and GLU-D1 in common wheat. For the GLU-A1 locus, many alleles have been described and collected in the Wheat Gene Catalogue (WGC). Many of those alleles have not been compared in conjunction and it is not possible to know if the current GLU-A1 variability described is real or it is overestimated. This study has focused on collecting all the germplasm associated with GLU-A1 variability, to verify the GLU-A1 allele of each accession and if the current variability described in the WGC is real. It was possible to collect and compare by SDS-PAGE most of the germplasm described in the WGC. The identity of many alleles was confirmed while there are other cases that should be reviewed in more detail. This study contributes to the correct classification of the GLU-A1 diversity and will promote the use of different alleles in future breeding programs aiming to improve gluten quality.