Fusca Lour Research Articles

Pharmacological, Phytochemical and Computational Studies on Prenylated Flavonoids and Pterocarpans From Erythrina Fusca Lour.

In the present study, the dry powdered bark of Erythrina fusca was extracted with methanol. It was subjected to peripheral analgesic study using the acetic acid-induced writhing test in mice model and it demonstrated significant analgesic activity. The methanol extract was partitioned into n-hexane, chloroform and water-soluble fractions. Primarily, the chloroform fraction of the methanol extract was subjected to various chromatographic techniques and four major metabolites, Lupinifolin (1), Lupinifolin-4’-methyl ether (2), Sandwicensin (3) and Licoagrocarpin (4) were purified. Molecular docking studies of 1-4 against cyclooxygenase-2 (COX-2) enzyme suggest that they may interact with COX-2 at different levels. The pharmacokinetic and biological parameters of 1-4 were also checked through in silico tool to understand their drug-likeliness. Bangladesh J. Bot. 53(2): 305-311, 2024 (June)

Exploring the therapeutic potential of ethanol extract of Erythrina fusca Lour. roots as an analgesic, antipyretic, and anti-inflammatory agent in experimental animals

Erythrina fusca Lour., renowned in traditional medicine for its soothing, analgesic, anti-inflammatory, and neuroinhibitory properties, is the focus of our investigation. Expanding on its traditional applications, we assessed the analgesic, anti-inflammatory, and antipyretic characteristics of the ethanol extract from E. fusca root (REEF). Administered orally to mice at varying doses (50, 100, and 150 mg/kg for pain and fever reduction; 100, 150, and 200 mg/kg for anti-inflammatory experiments), REEF's effects were systematically evaluated through parameters such as reaction time, pain inhibition ratio, COX2 and PGE2 levels, rectal temperature, fever inhibition ratio, paw diameter, paw edema inhibition ratio, and inflammatory cytokines. Results reveal that REEF consistently reduced licking and biting time (p < 0.05) in response to formalin-induced pain in mice. Particularly noteworthy was the more pronounced reduction in COX2 and PGE2 during the late phase, with the most significant decrease observed at the 150 mg/kg REEF treatment (p < 0.05). The licking time graph substantiated the analgesic efficacy of REEF, illustrating simultaneous and sustained pain reduction over time. Moreover, REEF significantly alleviated fever (p < 0.05) in mice, with the most substantial effect observed three hours post-yeast injection. REEF not only efficiently reduced body temperature but also countered the fever-inducing effects of yeast at evaluated time points (p < 0.05). In the context of carrageenan-induced inflammation, paw swelling markedly diminished after 4 and 5 hours (p < 0.05). Notably, at the 5-hour mark, REEF treatment at 150 mg/kg dose exhibited significantly superior anti-inflammatory effects (p < 0.05). Additionally, mice treated with REEF (100, 150, and 200 mg/kg) exhibited a considerable decrease (p < 0.05) in serum concentrations of TNF-?, IL-1?, and IL-6 compared to the control treatment. The ethanol extract from Erythrina fusca roots demonstrates substantial potential for pain relief, fever reduction, and anti-inflammatory effects, positioning it as a promising herbal resource for the research and development of natural medicinal products with these therapeutic properties.

Molecular Docking and Dynamic Simulation of Erythrina fusca Lour Chemical Compounds Targeting VEGFR-2 Receptor for Anti-Liver Cancer Activity

Liver cancer is a serious health concern characterized by abnormal cell growth, but currently, available treatment options are limited, suggesting the need for a new therapeutic method. Therefore, this research aimed to investigate the potential of chemical compounds obtained from the cangkring plant (Erythrina fusca) as anti-liver cancer agents targeting Vascular Endothelial Growth Factor Receptor 2 (VEGFR-2). The investigation was conducted in silico through molecular docking and dynamic method. Molecular docking was performed using AutoDock Tools, followed by visualization with Biovia Discovery Studio. Additionally, molecular dynamics simulation was conducted using GROMACS software and visualized with Grace. A total of 36 chemical compounds from E. fusca were used as ligands for molecular docking. The results showed that Isobavachalcone (ISB) was the most effective test compound with a binding energy of -11.45 kcal/mol, compared to the positive control Sorafenib with a value of - 11.51 kcal/mol. In this context, hydrogen bonding, as well as hydrophobic, electrostatic, and unfavorable molecular interactions were identified. Moreover, molecular dynamics simulation provided RMSD, RMSF, Radius of Gyration (Rg), and hydrogen bond parameters. Analysis of these parameters further confirmed the superior stability of ISB in binding to VEGFR-2, suggesting the potential to suppress angiogenesis by blocking the receptor.

Phytochemical variation between hydrochloric and tartaric acid-derived alkaloidal extracts of Erythrina fusca Lour. leaves: A proton NMR-based approach

Erythrina fusca Lour. (Fabaceae) has been used traditionally to relieve toothache and migraine headaches, cleansing putrid ulcers, and reducing skin inflammations and irritation. Interestingly, most of the reported biological activities on the plant are attributed to its Erythrina alkaloids which are usually extracted through the acid-base method. While the selection of acids for the extraction process is crucial, there is limited information on comparing the efficacy of strong organic and inorganic acids in extracting Erythrina alkaloids, and, more broadly, other types of alkaloids. In this study, comparison between hydrochloric acid (HCl) and tartaric acid (H2T) was made to extract the Erythrina alkaloids. From the same mass (10 g) of the crude methanolic leaf extract, a significantly higher (9-fold) alkaloidal yield was obtained using H2T compared to the HCl. The alkaloidal extracts displayed different proton nuclear magnetic resonance (1H NMR) profile where identification of their phytochemical constituents was done extensively through comparison with respective data of previously isolated compounds from the Erythrina species. Forty-seven phytochemicals were identified in the HCl extract and they were mostly alkaloids. While the H2T extract not only contained alkaloids, but also flavonoids, and pterocarpans. Cumulatively, 83 phytochemicals were identified from the extracts, including 35 alkaloids, 18 flavonoids, 14 pterocarpans, 9 terpenes, 3 phenols, and 4 saponins. Based on multivariate analysis (MVDA), the alkaloids erysodine (1), erysovine (2), erysopine (3), warangalone (39), calopocarpin (53), and dolichin A (60) were found in both alkaloidal extracts. The PLS-DA model further identified the variation in the two extracts where the HCl extract was characterized by the flavonoids lupinifolin (41) and erythrisenegalone (43). Conversely, the H2T extract contained a broader range of phytochemicals, including the alkaloids glucoerysodine (21) and erythratidinone (28), the flavonoids 5,3′-dihydroxy-4′‑methoxy-5′-(3-methyl-1,3-butadienyl)-2′′,2′′dimethylpyrano-[5,6:6,7]-isoflavanone (51), 5,3′-dihydroxy-5′-(3‑hydroxy-3-methyl-1-butenyl)-4′‑methoxy-2′′,2′′dimethylpyrano-[5,6:6,7]-isoflavanone (52), and catechin (82), the pterocarpans calopocarpin (53) and sandwicensin (63), the terpenes loliolide (70), ursolic acid (80), propyloxyamyrin (83), and stigmast-3-en-4-one (73), the saponins sigmoisides A, B, and E (75, 76, and 77), and the phenol methyl gallate (81). The present findings highlight the value of targeting the extraction process for alkaloids, in this instance Erythrina alkaloids, using different type of acids. Furthermore, these compounds may be considered potential markers that were selectively extracted through the acid-base extraction process.

Acute and sub-chronic toxicity studies of the ethanol extract of Erythrina fusca Lour. fruit via oral administration in mice

Erythrina fusca Lour. is a medicinal plant traditionally used in herbal medicine; however, there are no records of toxicity associated with the ethanol extract of E. fusca fruit (EtEF). The objective of this study was to assess the safety of EtEF through toxicity testing. Four groups of Swiss albino mice were employed, including a control group and three groups administered EtEF at doses of 1000, 3000, and 5000 mg/kg (single dose) and 100, 300, and 500 mg/kg (administered repeatedly for 90 days). Various parameters, including body weight, food and water consumption, hematological and biochemical parameters, relative organ weight, urine composition, and histopathology, were evaluated. No significant differences were observed in the tested groups compared to the control group, and there was no evidence of morphological or histopathological damage in the organs of mice treated with EtEF. This study affirms the safety of EtEF and establishes a foundation for further investigations into its utilization in traditional medicine.

Evaluation of Erythrina fuscaLour. as a pulping raw material

ABSTRACT This paper characterizes Erythrina fusca Lour. in terms of anatomical, morphological and chemical properties to evaluate its efficacy as a pulping raw material. It is characterized with 47.0% α-cellulose and 28.8% lignin content with a fibre length of 1.11 mm. The vessels (pores) are distinct and form porous semi-rings, with a row of larger pores at the beginning of the growth layer, which facilitate penetration of chemicals during the cooking phase of pulping. Kraft pulping of E. fusca was carried out by varying cooking temperature, time and alkali charge. At optimum condition of 14% active alkali for 2 h of cooking at 170°C, pulp yield was 40% with kappa number of 26.6. The papermaking properties were also evaluated and showed tensile index of 52.4 N m g−1 with tear index of6.8 mN m2 g−1 at oSR of 57. This species can be used as a raw material for writing and printing grade pulp.

Assessment of Antioxidant, Antimicrobial, Cytotoxicity and Thrombolytic Potential of Erythrina fusca Lour Grown in Bangladesh

The current study was conducted to assess the antioxidant, antimicrobial, cytotoxicity and thrombolytic potential of the leaves and stem bark of Erythrina fusca Lour grown in Bangladesh. For the assessment of antioxidant properties, DPPH scavenging and phosphomolybdenum assays were used. In the DPPH free radical scavenging assay, the chloroform soluble fraction of a methanol extract of leaves of E. fusca exhibited the highest free radical scavenging activity with an IC50 value of 5.5 ± 0.60 μg/ml, while the reference standard (ascorbic acid) showed an IC50 value of 5.8 ± 0.18 μg/ml. All the test samples and the standard BHT demonstrated good linear relationship in the phosphomolybdenum assay. It was observed that the test samples contained considerable number of bioactive compounds including total phenolics in the aqueous soluble fraction of the leaves giving the highest 94.02 ± 1.004 GAE/100g of dried sample. All test samples exhibited moderate antimicrobial activity against 13 Gram-positive and Gram-negative bacterial strains and 3 fungi, where the chloroform soluble fraction of the stem bark demonstrated the highest inhibition of growth with zone of inhibition of 19.3 ± 0.21 mm against Bacillus cereus. All the test samples displayed cytotoxic activity against brine shrimp nauplii having significant LC50 and LC90 values. The chloroform soluble materials demonstrated highest lethality with LC50 value of 1.55 ± 0.30 μg/ml and LC90 65.68 ± 0.23 μg/ml. On the other hand, the carbon tetrachloride soluble fraction of the stem bark showed the highest clot lysis (22.75 ± 0.59%) as compared to that (66.77 ± 0.61%) revealed by reference standard, streptokinase. The findings of this study revealed that the leaves and stem bark of E. fusca growing in Bangladesh possesses bioactive compounds but further studies are required to purify and identify them. Bangladesh Pharmaceutical Journal 25(1): 1-8, 2022 (January)

Antibacterial Activity and Phytochemical Screening of Erythrina fusca Lour. Leaf Extract (Fabaceae)

Erythrina fusca Lour. (family Fabaceae) is a flowering tree that is locally known as ‘chengkering’. The plant is traditionally used in treatment of some symptoms related to bacterial infections such as wound infections, inflammations, and skin itching. This work reports for the first time in vitro antibacterial screening of the E. fusca Lour. methanolic leaf extract against some common Gram-negative and Gram-positive bacteria such as Klebsiella pneumonia, Proteus vulgaris, Pseudomonas aeruginosa, Bacillus cereus, Bacillus subtilis, and Staphylococcus aureus. The in vitro antibacterial assay was carried out using agar diffusion method with the extract concentration of 3 mg/well and vancomycin 30 µg/well as the positive control. It was found that the extract exhibits antibacterial effects in range of 10.5 – 14 mm of zone of inhibition (ZOI) against all tested bacteria except for K. pneumoniae. P. aeruginosa showing ZOI of 14 mm was the most sensitive bacterium while P. vulgaris (ZOI of 10.5 mm) was the least sensitive strain to the extract. Based on the preliminary phytochemical screening of using standard qualitative phytochemical tests, this species contains significant amount of alkaloids, flavonoids, terpenoids, and tannins, which might contribute to the antibacterial activity of the extract. Both antibacterial potential and presence of various phytochemicals in the extract could support the traditional uses of E. fusca , and the obtained results would serve as a basis for further exploration of antibacterial properties of the plant’s leaves.

Flavonoid, pterocarpans and steroid from Erythrina fusca Lour. growing in Bangladesh: isolation, and antimicrobial and free-radical scavenging activity

Flavonoid, pterocarpans and steroid from Erythrina fusca Lour. growing in Bangladesh: isolation, and antimicrobial and free-radical scavenging activity

Requirements for efficient plantlet regeneration using cotyledonary nodal explants of purple coral tree (Erythrina fusca Lour.)

There are over hundred species in the genus Erythrina which are of practical importance in multiple ways. The requirements for Erythrina plantlet regeneration in vitro were rarely studied. Surface-sterilized seeds of purple coral tree (Erythrina fusca Lour.) exhibited a high germination rate (80%) in vitro. The cotyledonary nodes from the one-month-old purple coral tree seedlings grown in vitro were used as explants. About 70% of the cotyledonary nodal explants exhibited shoot regeneration when the explants were cultured vertically with the cut ends inserted into basal Murashige and Skoog (MS) medium compared to only 10% of those placed lying horizontally on the surface of the medium. Supplementation of the basal MS medium with 1 mg L−1 of BAP, 1 mg L−1 kinetin and 1 mg L−1 TDZ resulted in the greatest shoot elongation and the highest number of shoots developed from the cotyledonary nodal explants. The regenerated shoots were able to root (100%) on basal MS medium while supplementation with 2 mg L−1 of either NAA or IBA was inhibitory to root elongation. This is the first study on plantlet regeneration of purple coral tree which seems to have more complex requirements for shoot regeneration in vitro than those reported for the other three Erythrina species. Since only 30% of the rooted in vitro shoots survived during acclimatization following a previously published protocol for acclimatization of another plant, it is recommended that future studies are to investigate the requirements of higher plantlet survival for the rooted in vitro shoots of purple coral tree.

BIRD COMMUNITY STRUCTURE IN MACROHABITATS OF THE AQUATICTERRESTRIAL TRANSITION ZONE IN THE PANTANAL WETLAND, BRAZIL

Ecological responses of bird communities in aquatic-terrestrial transition zones (ATTZs) are only partially understood. The aim of this study was to evaluate community structure of birds in different macrohabitats of ATTZs from a protected area in the Pantanal wetland, Brazil. The survey of birds was carried out in three types of macrohabitats: Erythrina fusca Lour. monospecific forest, shrubland and pioneer polyespecific forest, and flooded grassland. The bird community living within the ATTZ varied according to macrohabitat type and period of the wetland landscape. We emphasise the need to expand this protected area, and exert greater environmental control, since the region is not exempt from direct and indirect impacts derived from the Upper Paraguay River Basin.

Spatial distribution of single specie dominant forests of Erythrina fusca Lour. at the Taiamã Ecological Station, Pantanal, Mato Grosso, Brazil

The Pantanal biome has a great variety of habitats where plant species are distributed according to their particularities. This study aimed at evaluating the spatial distribution of arboreal/shrub vegetation in areas of single specie dominance Erythrina fusca forests. An aggregation test (K(t)) for E. fusca and spatial dependence tests (K(12)) between E. fusca and Calophyllum brasiliense, as well as Alchornea discolor and Banara arguta were performed. The correlation of the distance from the beginning of the plots (riverbank) to the abundance of individuals was tested, and finally, the Kernel density was estimated. We found that E. fusca is randomly distributed throughout the area, that the distribution of C. brasiliense and A. discolor is dependent on E. fusca, and that of B. arguta is not. The correlation analysis pointed to a decrease in the abundance of the community from the riverbank to the interior of the island, with a smaller reduction in the population of E. fusca. The density was higher in the regions close to the river. In addition, sites with a high concentration of individuals close to the elevations created by the roots of E. fusca were verified. The distribution of individuals was related to the flood pulse and the adaptive processes of plant establishment, where the flooding selects the species most tolerant to this type of environment, and the formation of higher microrelief in the landscape can increase the dispersion of the species. This points out that any disturbance that may occur in the flood pulse will change the environmental balance.

EL SACHA INCHI Y SU APORTE EN LA RECUPERACION DE SUELOS ALTERADOS EN LA SELVA

Currently, there are innumerable environmental technologies in the recovery of disturbed soils, including the use of leguminous plants that provide physical, chemical and biological improvements. The present investigation was based on evaluating the behavior of Sacha inchi (Plukenetia volubilis L. and Erythrina fusca Lour). under conditions of fertilization and densities and its contribution to the recovery of disturbed soils in the Monzón district. The method used was experimental in Design of Divided Plots (DPD) in Complete Blocks at Random 2 densities (DS1: 1666 and 1111 plants ha-1) x 4 (N1: 0-0-0, N2: 20-30-20, N3: 25-40-20 and N4: 40-60- 40) x 3 repetitions. The results indicate that the levels of fertilization N2, N3 and N4 produced a significant effect on the parameters of the vegetative characteristics of Plukenetia volubilis L, for the vegetative characteristics of Erythrina fusca evidence of AB interaction in the number of shoots at 5 months; the integration of both species achieved the improvement in the chemical properties of the soil. Like the reduction of aluminum by 30%, increase of p H by 5%, increase of potassium and phosphorus. Key words: Plukenetia volubilis L., Erythrina fusca Lour, fertilization, soil

PENENTUAN KADAR FENOLAT TOTAL DAN AKTIVITAS ANTIOKSIDAN DARI EKSTRAK DAUN DADAP MERAH (Erythrina fusca Lour) SECARA SPEKTROFOTOMETRI UV-Vis

Determination of total phenolate and antioxidant activity of red leaf extract (Erythrina fusca Lour) was done by UV-Vis spectrophotometry. This study aims to determine the total phenolic content and antioxidant activity of hexane, ethyl acetate and ethanol extract. The extracts were prepared using a non-polar-maseration method with hexane, ethyl acetate and ethanol solvents. The results showed total phenolic concentration using the Folin-Ciocalteu method were 0.412 g/100 g in the hexane extract, 1.782 g/100 g in the ethyl acetate extract and 5.455 g/100 g in the ethanol extract. Antioxidant activity conducted by using FRAP method (Ferric Reducing Antioxidant Power) were obtained 0,682 mmol Fe (II)/100 g at hexane extract, 5,186 mmol Fe (II)/100 g at ethyl acetate extract and 10,591 mmol Fe (II)/100 g on the ethanol extract. The antioxidant activity of gallic acid as standard was 44.356 g mmol Fe (II)/100g.

PENENTUAN KADAR FENOLAT TOTAL DAN AKTIVITAS ANTIOKSIDAN DARI EKSTRAK DAUN DADAP MERAH (Erythrina fusca Lour) SECARA SPEKTROFOTOMETRI UV-Vis

Determination of total phenolate and antioxidant activity of red leaf extract (Erythrina fusca Lour) was done by UV-Vis spectrophotometry. This study aims to determine the total phenolic content and antioxidant activity of hexane, ethyl acetate and ethanol extract. The extracts were prepared using a non-polar-maseration method with hexane, ethyl acetate and ethanol solvents. The results showed total phenolic concentration using the Folin-Ciocalteu method were 0.412 g/100 g in the hexane extract, 1.782 g/100 g in the ethyl acetate extract and 5.455 g/100 g in the ethanol extract. Antioxidant activity conducted by using FRAP method (Ferric Reducing Antioxidant Power) were obtained 0,682 mmol Fe (II)/100 g at hexane extract, 5,186 mmol Fe (II)/100 g at ethyl acetate extract and 10,591 mmol Fe (II)/100 g on the ethanol extract. The antioxidant activity of gallic acid as standard was 44.356 g mmol Fe (II)/100g.

Beneficial effect of medicinal plants on the contractility of post-hypoxic isolated guinea pig atria – Potential implications for the treatment of ischemic–reperfusion injury

Context Ischemic–reperfusion injury is accompanied by a decreased contractility of the myocardium. Positive-inotropic agents have proven useful for treating this condition but may exert serious side-effects.Objective In this study, aqueous preparations from Abelmoschus esculentus L. Moench (Malvaceae), Annona muricata L. (Annonaceae), Bixa orellana L. (Bixaceae), Cecropia peltata L. (Moraceae), Erythrina fusca Lour. (Fabaceae), Psidium guajava L. (Myrtaceae) and Terminalia catappa L. (Combretaceae) were evaluated for their ability to improve the decreased contractility of isolated guinea pig atria after hypoxic stress.Materials and methods Guinea pig atria isolated in Ringer-Locke buffer gassed with 100% O2 at 30 °C were exposed for 5 min to hypoxia, then allowed to recover in oxygenated buffer alone or containing a single plant extract (0.001–1 mg/mL). The contractility (g/s) and beating frequency (beats/min), as well as troponin C contents of the bathing solution (ng/mL), were determined and expressed as means ± SDs.Results The extracts of A. muricata, B. orellana, C. peltata and T. catappa caused an increase in the contractility compared to untreated atria of 340 ± 102%, 151 ± 13%, 141 ± 14% and 238 ± 44%, respectively. However, the latter two preparations increased the troponin C contents of the bathing solution to 36 ± 11 and 69 ± 33, compared to the value of 11 ± 3 ng/mL found with untreated atria.Conclusions Preparations from A. muricata and B. orellana may possess positive-inotropic properties which may improve the contractility of the post-hypoxic myocardium. Studies to assess their usefulness in ischemic–reperfusion injury are warranted.

Antiproliferative Effect of Chloroform Extract of Cangkring Leaves (Erythrina fusca Lour.) and Its Isolates against HeLa Cells

Cervical cancer is a type of cancer possessing the 3rd highest incidence among women worldwide. Today’s therapies against cancer are still ineffective, thus people are seeking for alternative method. Erythrina fusca Lour. has been used traditionally as anticancer. This experiment is conducted to study the ability of chloroform extract of E. fusca Lour. leaves and its isolates on HeLa cervical cancer cells. The cytotoxicity assay and doubling time assay were done using direct counting method, while the DNA staining assay was done using acrydine orange. The chloroform extract and isolate # 30 showed cytotoxic activity with IC50 of 16 and 5 µg/ml, respectively, while isolate # 2 and isolate # 8 didn’t. The effluent # 30 could not affect HeLa cells growth at the given concentration, but it might promote DNA fragmentation indicating apoptosis induction. The molecular mechanisms underlying these effects still need to be further explored.Keywords: Erythrina fusca Lour., isolate and effluent of chloroform extract, antiproliferative, cervical cancer

Fuscacarpans A–C, new pterocarpans from the stems of Erythrina fusca

Chemical investigation of the stems of Erythrina fusca Lour. led to the isolation of three new pterocarpans, named fuscacarpans A–C ( 1−3), together with fourteen known compounds, sandwicensin ( 4), erythribyssin A ( 5), erythrabissin I ( 6), demethylmedicarpin ( 7), eryvarin D ( 8), erypoegin I ( 9), hydroxycristacarpone ( 10), orientanol A ( 11), scandenone ( 12), genistein ( 13), liquiritigenin ( 14), isoliquiritigenin ( 15), vestitone ( 16) and 3,7,4′-trihydroxyflavone ( 17). Structures 1−3 were elucidated by spectroscopic and chemical methods. The isolates were evaluated for antibacterial, antiplasmodial and cytotoxic activities.

Effect of ethanol extract of Erythrina fusca Lour (cangkring) leaves on suppression of cyclooxygenase-2 expression in raji cell line

COX-2. COX-2 is expressed highly in inflammation and cancer cells. The effect of ethanol extract of Erythrina fusca Lour (cangkring) toward suppression of COX-2 expression in Raji cells was studied using immunocytochemistry method. This research showed that ethanol extract of E. fusca Lour leaves on concentration of 250,0; 125,0; dan 62,50 μg/mL was able to suppress the COX-2 expression significantly in comparison to control, with the inhibition of 70,19 ± 2,14 %; 44,69 ± 1,62 %; and 23,25 ± 2,21 %. Key words: COX-2 expression, anti-inflammation, anti cancer, Raji cells, ethanol extract of E. fusca leaves, immunocytochemistry

Antiproliferative Effect of The Bark and Leaves of Erythrina Fusca Lour Against HeLa Cells

Erythrina fusca Lour (Indonesian: Cangkring) has been traditionally used to cure skin virus infection, cancer and inflammation. This experiment, therefore, was conducted to test its ability as anticancer through its antiproliferative effect using HeLa examine cell line as model. The bark and leaves powder were extracted using ethanol (70 %) and were used in the experiment after freezed drying. Those bark and leaves extracts have LC50 values of 110 and 140 mg/ml respectively. Antiproliferative effect was tested by examining the doubling time effect of the extract against the proliferating cells. Three different concentration were used to treat the cells for each extract, and cells were counted within time courses. The growth profiles were then compared between treated and control cells by calculating the doubling times. Either bark and leaves extract treated cells showed prolongation of the doubling times. This result indicated that such extract possess inhibitory effect or Antiproliferative effect against HeLa cells. Morphology analysis and nuclear staining of the cells indicated that one of the mechanism of the antiproliferative effect possibly through cell cycle modulation and apoptotic pathway. Keywords : Erythrina fusca, Antiproliferative, Doubling time, HeLa Cell