Grain mold, caused by several species of fungi, reduces grain yield and quality. This study examined the effect of the two most common grain mold fungi, Fusarium thapsinum and Curvularia lunata, inoculated singly and together on germination and seed mycoflora. Germination rates for the different cultivars have been reported in details previously. C. lunata was the most frequently isolated fungal species followed by F. thapsinum and F. semitectum. Non-inoculated control samples had Alternaria spp. and F. semitectum as the most frequently recovered fungal species followed by C. lunata. There were highly significant negative correlations between germination and C. lunata (r = - 0.44 and P < 0.01) in 2000 and between germination and F. thapsinum (r = −0.52 and P < 0.01) in 2001. Significant negative correlations between F. semitectum andF. thapsinum, and highly significant negative correlations between F. thapsinum and C. lunata were observed. Data from this study indicate that the fungal species present, the environment, and the sorghum cultivar all influence seed mycoflora.