Fungal Community Succession Research Articles

Redistribution of phosphorus fraction driven by organic carbon and microbial community during composting

Available information on the coupling relationship between phosphorus fraction and organic carbon during composting remains limited. Thus, this research investigated the changes of phosphorus fraction, dissolved organic carbon fluorescent components and microbial community in swine manure composting with different carbon sources including the maize straw (MS), garden waste (GW) and distillers’ grains (DG), in order to investigate whether the distribution and availability of phosphorus are influenced by different carbon sources used in the composting of swine manure. The result showed that different carbon sources changed phosphorus availability variously mainly by altering the succession of fungal communities and phosphorus functional genes. The dissolved organic material including tyrosine and tryptophan facilitate the mineralization of organic phosphorus (Org-P) into water-soluble phosphorus, thereby improving phosphorus availability. However, humic acid-like carbon components promote the conversion of inorganic-phosphorus to Org-P, which is the direct cause of the reduced phosphorus availability during composting. The results of this study provide support for the development of phosphorus-rich, stable, and clean compost products.

Analysis of wheat fungal community succession in traditional storage structures using Illumina MiSeq sequencing technology

The diversity of fungi in wheat with different deoxynivalenol (DON) content at various periods post-harvest and in the environment of storage were investigated. The changes in DON content were measured with ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS), and an amplicon sequence analysis of fungi was performed in traditional storage structures using high-throughput sequencing. The changes in temperature, humidity, and CO2 concentration were collected by sensors. In addition, we analyzed principal component analysis, species composition, species differences, and community differences of fungi. There was an obvious separation of the fungal communities under different storage conditions and times. Many fungal genera were gradually decreasing during storage and were eventually undetectable, and many fungal genera that were undetectable at first gradually increased during storage and even became dominant fungal genera. The competition between fungi was fierce. The competition between fungi were affected by the presence of DON. As the initial DON content increased, the contribution of inter-group differences became more obvious. The temperature, humidity, and CO2 concentration of wheat in the silo's environment changed with extended storage time. The content of DON decreased with extended storage time. We had investigated the changes in DON content and their correlation with the changes in fungal communities and environmental factors, which showed a high degree of correlation. This study offers theoretical justification for optimizing safe wheat grain in traditional storage conditions.

Response of Soil Fungal Community to Reforestation on Shifting Sand Dune in the Horqin Sandy Land, Northeast China.

Reforestation of native shrub on shifting sand dunes has been widely used for desertification control in semi-arid grassland in Northeast China. Previous studies have confirmed that plantation establishment facilitates fixing sand dunes, restoring vegetation, and improving soil properties, but very few have focused on the response of the soil fungal community. In this study, a chronosequence of Caragana microphylla (CM) shrub sand-fixation plantations (8-, 19-, and 33-year-old), non-vegetated shifting sand dunes (0 years), and adjacent natural CM forests (NCFs; 50-year-old) in the Horqin sandy land were selected as experimental sites. Soil properties including enzymatic activities were determined, and the composition and structure of the soil fungal community were investigated using the Illumina MiSeq sequencing technique based on the internal transcribed spacer (ITS) rDNA. This study aimed to (1) describe the response of the soil fungal community to revegetation onto a moving sand dune by planting a native shrub plantation; (2) determine the main soil factors driving the succession of the fungal community; and (3) discuss whether the soil fungal community can be restored to its original state by reforestation. The reforestation of CM significantly ameliorated soil properties, increased soil fungal diversity, and altered the composition and structure of the soil fungal community. Ascomycota, Basidiomycota, and Zoopagomycota were the dominant phyla in all sites. Ascomycota did not respond to plantation development, whereas the other two dominant phyla linearly increased or decreased with the plantation age. The relative abundance of dominant genera varied with sites and showed a waning and waxing characteristic. The composition and structure of the soil fungal community in the 33-year CM plantation were very close to that of the NCF, indicating the restorability of the soil fungal community. The succession of the soil fungal community was directly driven by soil properties, of which soil moisture, organic matter, total N, urease, and protease were the main affecting factors.

Effects of small-scale outplanting fertilization on conifer seedling growth and fungal community establishment

Forestry in Sweden largely relies on planting genetically improved seedlings after clear-cutting, and high survival and early growth of planted seedlings is vital for stand establishment, economic viability, and carbon sequestration. Norway spruce (Picea abies) and Scots pine (Pinus sylvestris) are the two most important tree species in Swedish forest stands and both are associated with a variety of ectomycorrhizal fungi. While seedlings are generally not fertilized at outplanting, previous results have shown that fertilization with arginine phosphate can increase root growth and seedling survival. However, it is not clear how this affects fungal community composition on the roots of growing seedlings. In a planting experiment sampled after one and two growing seasons, we found that planting position had the largest effects both on seedling performance and on fungal community composition and provide insight into the early stages of fungal community succession on planted Norway spruce and Scots pine seedlings. Fungal taxa present on seedlings before planting persisted on seedling roots, while some degree of novel colonization by site indigenous taxa was observed. Fertilization modified the relative abundance of some fungal taxa but did not lead to significant changes in overall community composition. In terms of seedling performance, ammonium nitrate led to increased mortality while arginine phosphate improved root growth.

Temporal Dynamics of Fungal Communities in Alkali-Treated Round Bamboo Deterioration under Natural Weathering.

Microbes naturally inhabit bamboo-based materials in outdoor environments, sequentially contributing to their deterioration. Fungi play a significant role in deterioration, especially in environments with abundant water and favorable temperatures. Alkali treatment is often employed in the pretreatment of round bamboo to change its natural elastic and aesthetic behaviors. However, little research has investigated the structure and dynamics of fungal communities on alkali-treated round bamboo during natural deterioration. In this work, high-throughput sequencing and multiple characterization methods were used to disclose the fungal community succession and characteristic alterations of alkali-treated round bamboo in both roofed and unroofed habitats throughout a 13-week deterioration period. In total, 192 fungal amplicon sequence variants (ASVs) from six phyla were identified. The fungal community richness of roofed bamboo samples declined, whereas that of unroofed bamboo samples increased during deterioration. The phyla Ascomycota and Basidiomycota exhibited dominance during the entire deterioration process in two distinct environments, and the relative abundance of them combined was more than 99%. A distinct shift in fungal communities from Basidiomycota dominant in the early stage to Ascomycota dominant in the late stage was observed, which may be attributed to the increase of moisture and temperature during succession and the effect of alkali treatment. Among all environmental factors, temperature contributed most to the variation in the fungal community. The surface of round bamboo underwent continuous destruction from fungi and environmental factors. The total amount of cell wall components in bamboo epidermis in both roofed and unroofed conditions presented a descending trend. The content of hemicellulose declined sharply by 8.3% and 11.1% under roofed and unroofed environments after 9 weeks of deterioration. In addition, the contact angle was reduced throughout the deterioration process in both roofed and unroofed samples, which might be attributed to wax layer removal and lignin degradation. This study provides theoretical support for the protection of round bamboo under natural weathering.

Relative Contribution of Fungal Communities to Carbon Loss and Humification Process in Algal Sludge Aerobic Composting

Harmful algal blooms in eutrophic lakes pose significant challenges to the aquatic environment. Aerobic composting is an effectively method for processing and reusing dewatered algal sludge. The fungal communities are the main driver of composting. However, their relationship with carbon loss and the humification process during algal sludge composting remains unclear. In this study, the succession of fungal communities in algal sludge composting was investigated via internal transcribed spacer (ITS) rRNA amplicon sequencing analysis. Overall, no significant differences were observed with the α-diversity of fungal communities at different stages. The composition of the fungal communities changed significantly before and after compost maturation and became more stable after the compost maturation. Redundancy analysis showed that the fungal communities were significantly correlated with physicochemical properties, including humic acid (HA)/fulvic acid (FA), temperature, pH, humic acid, microcystins, and CO2. The co-occurrence network showed that different fungal community modules had different relationships with physicochemical properties. Structural equation modeling further revealed that different metabolic or transformation processes may be mainly driven by different fungi modules. The microcystin degradation, carbon loss, and humification during composting were mainly mediated by fungal communities which were mainly influenced by temperature. Humification was influenced not only by fungal communities but also by the microcystin levels. These results show that changes in the fungal community composition and interaction and their relationship with physicochemical properties could represent a useful guide for optimizing the composting process.

Succession of bacterial and fungal communities during the mud solarization of salt-making processing in a 1000-year-old marine solar saltern

A 1000-year-old marine solar saltern in Hainan Island, South China, is in the continuation of traditional skills involving mud soak and solarization for three days to accumulate salt, which is a unique process contributing to the selection of highly halophilic microbes related to salt quality and flavor. Herein, the successions of physicochemical properties and bacterial and fungal communities of muds from the saltern were investigated during the processing of mud solarization. The results showed that Na+, Cl−, Mg2+ and total nitrogen were the main factors in explaining the fluctuation of the fungal community, whereas the bacterial community was predominantly determined by Ca2+, total kalium and total phosphorus. During three days of mud solarization, the soil bacterial alpha diversity in the saltern was significantly lower than that of the surroundings, whereas the fungal alpha diversity showed an opposite trend. The bacterial functional groups with the same survival and adaptation strategies remained relatively stable, while the dominant functional type in fungi changed from monotropic to complex trophic mode. Additionally, some unidentified fungi species were increasingly found in mud. The abundances of Saitozyma, Solicoccozyma and Wallemia (Basidiomycota) increased and may contribute to the salt flavor through decomposing cellulose molecules into glucose due to their higher β-glucosidase activity, while the harmful fungal genera including Inocybe and Paraconiothyrium decreased obviously due to the stresses of salinity and solar exposure. These results showed that the traditional salt-making skills associated with mud solarization have selected a high abundance of beneficial halophilic microbes to increase the salt quality and flavor. Our findings not only reveal the underlying mechanisms for the long-history salt-making skills of this 1000-year-old unusual cultural heritage but also are highly valuable for bioprospecting to develop novel halophilic bacteria and fungi for modern chemistry industries.

Fungal Community Succession and Volatile Compound Changes during Fermentation of Laobaigan Baijiu from Chinese Hengshui Region.

To investigate the core fungal community succession and its effects of volatile compound production during different stages (D-1, D-2, D-3, E-4, E-5, and E-6) of Hengshui Laobaigan Baijiu, high-throughput sequencing (HTS) was carried out, accompanied by the identification and quantification of the volatile flavor compounds using headspace solid-phase coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS). HTS results demonstrated that the fungal community of stage D-1 was similar to that of E-4 after adding Daqu, while the richness and diversity of the fungal community were most prominent at stage E-6. Moreover, the addition of Daqu at the beginning of Ercha fermentation resulted in a significant increase in the relative abundances of the fungal community at the genus level, setting the stage for the production of volatile compounds. GC-MS analysis revealed the presence of a total of 45 volatile compounds. Combining the GC-MS result with the heat map and principal component analysis (PCA), the concentrations of volatile compounds were highest in stage E-5. Meanwhile, concentrations of esters, especially ethyl acetate, ethyl lactate, isoamyl acetate and ethyl hexanoate, were high in both stages E-5 and E-6. This indicated that stage E-5 was crucial to the fermentation process of Laobaigan Baijiu. Three fungal genera (Saccharomyces, Candida, and Pichia) were indicated as the core microbiota for the production of the main volatile flavor compounds of Laobaigan Baijiu through partial least square (PLS) analysis. The information provided in this study offered valuable insights into the fermentation mechanism of Laobaigan Baijiu, thereby serving as a theoretical framework for enhancing the quality of Baijiu and realizing cost-effective production.

Biochar addition accelerates the humification process by affecting the microbial community during human excreta composting

ABSTRACT Biochar addition plays an important role in manure composting, but its driving mechanism on microbial succession and humification process of human excreta composting is still unclear. In the present study, the mechanism of biochar addition was explored by analysing the humification process and microbial succession pattern of human excreta aerobic composting without and with 10% biochar (HF and BHF). Results indicated that BHF improved composting temperature, advanced the thermophilic phase by 1 d, increased the germination index by 49.03%, promoted the growth rate of humic acid content by 17.46%, and raised the compost product with the ratio of humic acid to fulvic acid (HA/FA) by 16.19%. Biochar regulated the diversity of fungi and bacteria, increasing the relative abundance of Planifilum, Meyerozyma and Melanocarpus in the thermophilic phase, and Saccharomonospora, Flavobacterium, Thermomyces and Remersonia in the mature phase, which accelerates the humification. Bacterial communities’ succession had an obvious correlation with the total carbon, total nitrogen, and temperature (P < 0.05), while the succession of fungal communities was influenced by the HA/FA and pH (P < 0.05). This study could provide a reference for the improvement of on-site human excreta harmless by extending the thermophilic phase, and facilitating the humification in human excreta compost with biochar addition.

Humification and fungal community succession during pig manure composting: Membrane covering and mature compost addition

The objective of this study was to elucidate the combined effect of a semi-permeable membrane (M) and mature compost (MC) on humification and fungal community succession in pig manure composting. Compared with the control, the concentrations of humic substances (HSs) increased by 44.54 % (M + 15 % MC) and 43.90 % (M). During the thermophilic phase, Aspergillus (67.26 %) was the dominant genus in the M + 15 % MC treatment. Membrane covering increased the relative abundance (RA) of other phyla (except for Ascomycetes and Basidiomycetes) on the 14th day and Basidiomycetes on the 80th day in M treatment. Humic acid, HSs were positively correlated with the RA of genera Myceliophthora, Kernia, and Mycothermus. Myceliophthora was the key genus in the M + 15 % MC treatment on the 80th day. The results showed that 15 % MC addition under membrane covering optimizes the quality of composting products.

Effect of Aspergillus flavus contamination on the fungal community succession, mycotoxin production and storage quality of maize kernels at various temperatures

Aspergillus flavus, a notorious saprobe and opportunistic plant pathogen, alters mycotoxin contamination and biochemical components in maize kernels during processing and storage, thereby reducing the possibilities of maize end use and compromising food safety. This study explored changes in mycotoxin production, fungal community succession and biochemical components in maize kernels stored at 20, 25 and 30 °C, exposed to A. flavus. Results showed that aflatoxin B1 concentration increased over time, reaching 4.88 μg/kg at 20 °C, 167.23 μg/kg at 25 °C and 349.64 μg/kg at 30 °C after 15 days of storage, whereas the zearalenone production was characterized by an increase followed by a decrease. Correspondingly, the number of molds gradually increased and reached a stable stage after 10 days. High-throughput sequencing of the internal transcribed spacer (ITS) revealed that Eurotium dominated the fungal communities, with A. flavus reaching maximum abundance in maize kernels stored at 30 °C for 15 days. Correlation analysis indicated that the relative abundance of A. flavus was significantly negatively correlated with the content of zein and moisture (P < 0.05). Moreover, the wet milling process of maize effectively eliminated the concentration of aflatoxin B1 and zearalenone from the starch. Pasting temperature and setback value of starch decreased while peak viscosity, final viscosity and breakdown value increased with storage. These findings indicate that interactions between the epiphytic fungal community and A. flavus at elevated storage temperatures aggravate both maize quality deterioration and mycotoxin contamination. Furthermore, they have a discernible impact on the pasting properties of starch. This insight informs strategies to control fungal infections during maize processing and storage.

Correlation between fungi and volatile compounds during different fermentation modes at the industrial scale of Merlot wines

Elucidation of the relationship between fungal community development and dynamic changes in volatile components during fermentation is of great significance in controlling wine production. However, such studies on an industrial scale are rarely reported. In this study, fungal community succession during spontaneous fermentation (SPF) and inoculation fermentation (INF) of Merlot wine was monitored by a research strategy combining culture-dependent and culture-independent methods. The volatile compounds were monitored during SPF and INF by headspace solid-phase micro-extraction coupled with gas chromatography-mass spectrometry technology. The Spearman correlation coefficient was also used to investigate the interplay between fungal communities and volatile compounds. We found that fungal community diversity in SPF decreased as fermentation progressed but was significantly higher than that of INF. Starmerella and Kazachstania were the dominant non-Saccharomyces genera in Merlot wine during SPF. However, the presence of commercial yeasts and sulphur dioxide led to a sharp decrease or the disappearance of non-Saccharomyces genera during INF. Spearman correlation analysis revealed that all major volatiles were positively correlated with most functional microbiotas except P. fermentans, S. bacillaris, E. necator, and D. exigua in INF. In SPF, most non-Saccharomyces were negatively correlated with core volatiles, whereas K. humilis, M. laxa, P. kluyveri, and A. japonicus were positively correlated with the major volatiles, especially some higher alcohols (isopentol, heptanol) and terpenes (linalool, citronellol). S. cerevisiae was positively correlated with most of the main volatile substances except ethyl isovalerate and isoamyl acetate. These findings provide a reference for comprehending the diverse fermentation methods employed in the wine industry and improving the quality of Merlot wines.

Fungal Community Succession of Populus grandidentata (Bigtooth Aspen) during Wood Decomposition

Fungal communities are primary decomposers of detritus, including coarse woody debris (CWD). We investigated the succession of fungal decomposer communities in CWD through different stages of decay in the wide-ranging and early successional tree species Populus grandidentata (bigtooth aspen). We compared shifts in fungal communities over time with concurrent changes in substrate chemistry and in bacterial community composition, the latter deriving from an earlier study of the same system. We found that fungal communities were highly dynamic during the stages of CWD decay, rapidly colonizing standing dead trees and gradually changing in composition until the late stages of decomposed wood were integrated into soil organic matter. Fungal communities were most similar to neighboring stages of decay, with fungal diversity, abundance, and enzyme activity positively related to percent nitrogen, irrespective of decay class. In contrast to other studies, we found that species diversity remained unchanged across decay classes. Differences in enzyme profiles across CWD decay stages mirrored changes in carbon recalcitrance, as B-D-xylosidase, peroxidase, and Leucyl aminopeptidase activity increased as decomposition progressed. Finally, fungal and bacterial gene abundances were stable and increased, respectively, with the extent of CWD decay, suggesting that fungal-driven decomposition was associated with shifting community composition and associated enzyme functions rather than fungal quantities.

Succession of Fungal Communities and Their Functional Profiles in a Decaying Foundation Species.

Dead plant biomass from foundation plant species is fundamental for the survival of coastal salt marshes because dead biomass aids in the vertical accretion of the ecosystem. Fungi regulate the decomposition of dead biomass, and thus play an essential role for marsh survival. Despite their importance, little is known about the compositional and functional changes of fungal communities in plant matter throughout senescence and litter decomposition. Here, we analyzed how fungal communities and their functionality change in the foundation plant species Spartina patens, which builds vast amounts of dead plant biomass (thatch) on the soil surface. We analyzed the chemical and fungal properties of live biomass, standing dead biomass (dead biomass shortly after senescence), upper thatch (top layer of litter on the soil surface), and lower thatch (bottom layer of litter on the soil surface) during September and November of 2021. We found that the chemical and fungal properties of different S. patens biomass types followed a predictable litter decomposition pattern. Notably, live biomass, standing dead biomass, upper thatch, and lower thatch all hosted unique fungal communities and litter chemistry. Functional groups present in live biomass (pathogens, epiphytes, and mycoparasites) were lost during senescence and later replaced by diverse saprotrophs. The abundance of lignocellulose saprotrophs increased throughout decomposition, with the highest abundance occurring in lower thatch. These results suggest a predictable succession of fungal communities through the senescence and decomposition of the foundation species S. patens. Our study highlights the diversity of fungal communities in a disappearing foundation species.

Changes in Community Structure and Functional Characteristics of Soil Bacteria and Fungi along Karst Vegetation Succession

Soil microbes are a crucial component of karst ecosystems, and exploring their community changes during succession can help to elucidate the mechanisms driving succession dynamics. However, the variation of soil microbial communities during vegetation succession in karst ecosystems is still poorly understood. We studied the variations in community structure and potential functions of soil microbes within the four successional stages of grassland (GL), shrubland (SL), secondary forest (SF), and primary forest (PF) for the topsoil (0–10 cm) and subsoil (10–20 cm) in a karst area using high-throughput sequencing. The research findings showed that the bacterial and fungal community diversity and composition changed more obviously in the topsoil than in the subsoil across the succession. With vegetation succession, the structural and functional characteristics of soil bacterial and fungal communities show different trends, with soil fungal communities having a greater response to successional stage changes. Actinobacteria and Acidobacteria were dominant in secondary and primary forests, respectively, while Bacteroidetes was prevalent in grassland. However, the change in Proteobacteria was not significant at both soil depths. Ascomycota was the dominant phylum of soil fungi throughout the succession. The function of soil bacteria was mainly carbohydrate metabolism, which had the highest proportion in the shrubland at different soil depths. The dominant fungal functional groups were saprotroph, pathotroph, and pathotroph–saprotroph. The soil bacterial communities were observably affected by soil organic carbon, total nitrogen, total potassium, ammonia nitrogen, nitrate nitrogen, and leucine aminopeptidase, among which soil organic carbon, ammonia nitrogen, and leucine aminopeptidase mainly influenced the bacterial community in the topsoil, while nitrate nitrogen chiefly influenced the bacterial community in the subsoil. The soil fungal community was only significantly affected by soil organic carbon. Collectively, these results indicate that the effects of vegetation succession on soil microbial communities are largely driven by successional stage and soil properties, with soil fungi being more susceptible to the vegetation successional stage and soil bacteria being more sensitive to the soil properties. During this process, soil bacterial and fungal communities follow different succession patterns.

Influence of biochar on succession of fungal communities during food waste composting

This study aims to examine the effects of biochar on fungal dynamics during food waste composting. The different dosage of wheat straw biochar from 0 to 15% (0%, 2.5%, 5%, 7.5%, 10%, and 15%) were used as an additive to composting and examined for 42 days. The results showed that Ascomycota (94.64%) and Basidiomycota (5.36%) were the most dominant phyla. The most common fungal genera were Kluyveromyces (3.76%), Candida (5.34%), Trichoderma (2.30%), Fusarium (0.46%), Mycothermus-thermophilus (5.67%), Trametes (0.46%), and Trichosporon (3.38%). The average number of operational taxonomic units were 469, with the greatest abundance seen in the 7.5% and 10% treatments. Redundancy analysis revealed that different concentrations of biochar applied treatments have significantly distinct fungal communities. Additionally, correlation analyses of fungal interactions with environmental elements, performed through a heatmap, also indicate a distinct difference among the treatments. The study clearly demonstrates that 15% of biochar has a positive impact on fungal diversity and improves the food waste composting.

Succession of Fungal Community during Outdoor Deterioration of Round Bamboo

Bamboo’s mechanical and aesthetic properties are significantly influenced by fungi. However, few studies have been conducted to investigate the structure and dynamics of fungal communities in bamboo during its natural deterioration. In this study, fungal community succession and characteristic variations of round bamboo in roofed and unroofed environments over a period of 13 weeks of deterioration were deciphered using high-throughput sequencing and multiple characterization methods. A total of 459 fungal Operational Taxonomic Units (OTUs) from eight phyla were identified. The fungal community’s richness of roofed bamboo samples showed an increasing trend, whereas that of unroofed bamboo samples presented a declining trend during deterioration. Ascomycota and Basidiomycota were the dominant phyla throughout the deterioration process in two different environments: Basidiomycota was found to be an early colonizer of unroofed bamboo samples. Principal Coordinates Analysis (PCoA) analysis suggested that the deterioration time had a greater impact on fungal community variation compared to the exposure conditions. Redundancy analysis (RDA) further revealed that temperature was a major environmental factor that contributed to the variation in fungal communities. Additionally, the bamboo epidermis presented a descending total amount of cell wall components in both roofed and unroofed conditions. The correlation analysis between the fungal community and relative abundance of three major cell wall components elucidated that Cladosporium was negatively correlated with hemicellulose in roofed samples, whereas they presented a positive correlation with hemicellulose and a negative correlation with lignin in unroofed samples. Furthermore, the contact angle decreased during the deterioration process in the roofed as well as unroofed samples, which could arise from the degradation of lignin. Our findings provide novel insights into the fungal community succession on round bamboo during its natural deterioration and give useful information for round bamboo protection.

Seasonal Succession of Fungal Communities in Native Truffle (Tuber indicum) Ecosystems.

Truffles are a rare underground fungus and one of the most expensive, and sought-after kitchen ingredients in the world. Microbial ecology plays an important role in the annual growth cycle of truffles, but fungal communities in native truffle ecosystems are still largely unknown, especially for Tuber indicum from China. In this study, the spatial and temporal dynamics of soil physicochemical properties and fungal communities were described associated with four T. indicum-producing plots (TPPs) and one non-truffle-producing plot in four successive growing seasons. A total of 160 biological samples were collected, 80 of which were used for the determination of 10 soil physicochemical indices and 80 for Illumina-based analysis of the fungal microbiome. Soil physicochemical properties and fungal communities exhibited considerable seasonal variation. Ascomycetes, Basidiomycetes, and Mucormycoides dominated. The core microbiome work on the microecological changes in TPPs, and the identified core members contribute to the seasonal succession of communities. The genus Tuber occupies a central position in healthy TPPs. There was a strong correlation between soil physicochemical properties and fungal communities. The genus Tuber showed a positive correlation with Ca, Mg, and total nitrogen, but a negative correlation with total phosphorus and available potassium. This study describes the complex ecological dynamics of soil physicochemical indices and fungal communities occurring during the annual cycle of Tuber indicum, and highlights the succession of core communities in truffle plots, which contribute to better protection of native truffle ecosystems and control of mycorrhizal fungal contamination in artificial truffle plantations in China. IMPORTANCE The spatial and temporal dynamics of soil physicochemical properties and fungal communities associated with four Tuber indicum-producing plots and one non truffle producing plot in four different growing seasons are described. Soil physicochemical properties and fungal communities exhibited considerable seasonal variation. This study examines the complex ecological dynamics of soil physicochemical indices and fungal communities occurring during the annual cycle of Tuber indicum and highlights the succession of core communities in truffle plots, which contributes to better protection of native truffle ecosystems and control of mycorrhizal fungal contamination in artificial truffle plantations in China.

Regulating pH and Phanerochaete chrysosporium inoculation improved the humification and succession of fungal community at the cooling stage of composting

This study aimed to explore the effect of regulating pH and Phanerochaete chrysosporium inoculation at the cooling stage of composting on the lignocellulose degradation, humification process and related precursors as well as fungal community for secondary fermentation. Results showed that composting with P. chrysosporium inoculation and pH regulation (T4) had 58% cellulose decomposition, 73% lignin degradation and improved enzyme activities for lignin decomposition. There was 81.98% increase of humic substance content and more transformation of polyphenols and amino acids in T4 compared to control. Inoculating P. chrysosporium affected the fungal community diversity, and regulating pH helped to increase the colonization of P. chrysosporium. Network analysis showed that the network complexity and synergy between microorganisms was improved in T4. Correlation and Random Forest analysis suggested that enriched Phanerochaete and Thermomyces in the mature stage of T4 were key taxa for lignocellulose degradation, and humic acid formation by accumulating precursors.

Comparing the succession of microbial communities throughout development in field and laboratory nests of the ambrosia beetle Xyleborinus saxesenii.

Some fungus-farming ambrosia beetles rely on multiple nutritional cultivars (Ascomycota: Ophiostomatales and/or yeasts) that seem to change in relative abundance over time. The succession of these fungi could benefit beetle hosts by optimal consumption of the substrate and extended longevity of the nest. However, abundances of fungal cultivars and other symbionts are poorly known and their culture-independent quantification over development has been studied in only a single species. Here, for the first time, we compared the diversity and succession of both fungal and bacterial communities of fungus gardens in the fruit-tree pinhole borer, Xyleborinus saxesenii, from field and laboratory nests over time. By amplicon sequencing of probed fungus gardens of both nest types at three development phases we showed an extreme reduction of diversity in both bacterial and fungal symbionts in laboratory nests. Furthermore, we observed a general transition from nutritional to non-beneficial fungal symbionts during beetle development. While one known nutritional mutualist, Raffaelea canadensis, was occurring more or less stable over time, the second mutualist R. sulphurea was dominating young nests and decreased in abundance at the expense of other secondary fungi. The quicker the succession proceeded, the slower offspring beetles developed, suggesting a negative role of these secondary symbionts. Finally, we found signs of transgenerational costs of late dispersal for daughters, possibly as early dispersers transmitted and started their own nests with less of the non-beneficial taxa. Future studies should focus on the functional roles of the few bacterial taxa that were present in both field and laboratory nests.