This paper presents systematic experiments carried out to study the variation in amplitude of the fundamental symmetric Lamb mode (So) due to misalignments in air-coupled transducers. In this work three misalignments—linear, orientation and synchronized orientation were intentionally introduced in the air-coupled transducers, and experiments were carried out on two glass fiber reinforced plastic laminates of different ply stacking. A-scans were captured positioning the air-coupled probes in several configurations over the laminates. In each misalignment case, variation in amplitude of So mode with respect to misalignment variable was plotted. Curves were fitted in those misalignment cases where there was sufficient experimental data. Interestingly, it was observed that Gaussian curves accurately characterize the variation in amplitude with respect to the misalignment variable.