Permeability Function Research Articles

Functional recovery outcomes following acute stroke is associated with abundance of gut microbiota related to inflammation, butyrate and secondary bile acid.

Accumulating evidence suggests that gut microbes modulate brain plasticity via the bidirectional gut-brain axis and play a role in stroke rehabilitation. However, the microbial species alterations associated with stroke and their correlation with functional outcome measures following acute stroke remain unknown. Here we measure post-stroke gut dysbiosis and how it correlates with gut permeability and cognitive functions in 12 stroke participants, 18 controls with risk factors for stroke, and 12 controls without risk factors. Stool samples were used to measure the microbiome with whole genome shotgun sequencing and leaky gut markers. We genotyped APOE status and measured diet composition and motor, cognitive, and emotional status using NIH Toolbox. We used linear regression methods to identify gut microbial associations with cognitive and emotional assessments. We did not find significance differences between the two control groups. In contrast, the bacteria populations of the Stroke group were statistically dissimilar from the control groups. Relative abundance analysis revealed notable decreases in butyrate-producing microbial taxa, secondary bile acid-producing taxa, and equol-producing taxa. The Stroke group had higher levels of the leaky gut marker alpha-1-antitrypsin in the stool than either of the groups and several taxa including Roseburia species (a butyrate producer) were negatively correlated with alpha-1-antitrypsin. Stroke participants scored lower on memory testing than those in the two control groups. Stroke participants with more Roseburia performed better on the picture vocabulary task; more Bacteroides uniformis (a butyrate producer) and less Escherichia coli (a pro-inflammatory species) reported higher levels of self-efficacy. Intakes of fiber, fruit and vegetable were lower, but sweetened beverages were higher, in the Stroke group compared with controls. Vegetable consumption was correlated with many bacterial changes among the participants, but only the species Clostridium bolteae, a pro-inflammatory species, was significantly associated with stroke. Our findings indicate that stroke is associated with a higher abundance of proinflammatory species and a lower abundance of butyrate producers and secondary bile acid producers. These altered microbial communities are associated with poorer functional performances. Future studies targeting the gut microbiome should be developed to elucidate whether its manipulation could optimize rehabilitation and boost recovery.

Simultaneous interpretation of SCAL data with different degrees of freedom and uncertainty analysis

Multiphase flow in porous media is relevant in many areas of geoenergy engineering and is governed by relative permeability and capillary pressure saturation functions. These functions are key uncertainties in reservoir engineering and their measurement is demanding and resource intensive. Despite the experimental effort, it is not yet common practice to numerically interpret the data, nor is it common practice to investigate their uncertainty. Furthermore, data interpretation is limited to power-law functions, which is insufficient for describing complex rock types such as microscopically heterogeneous carbonates.We developed a MATLAB-MRST-based simulator for simultaneous interpretation of data sets from different experimental techniques. We discuss the implementation of the common parametrized relative permeability representations and their deficiency to describe data from complex rocks. To overcome this limitation, a point-by-point approach is developed and applied to an extensive carbonate data set. For uncertainty analysis, a Markov Chain Monte Carlo sampling-based workflow is implemented and applied. The uncertainty is discussed in the frame of the individual data set, simultaneously analyzed data sets, and the sample-to-sample variation. The developed method makes the interpretation of relative permeability and capillary pressure saturation functions conclusive, especially in cases of complex line shapes, and is an essential step toward uncertainty-driven stochastic reservoir modeling.

Recent advances in surface modification of micro- and nano-scale biomaterials with biological membranes and biomolecules.

Surface modification of biomaterial can improve its biocompatibility and add new biofunctions, such as targeting specific tissues, communication with cells, and modulation of intracellular trafficking. Here, we summarize the use of various natural materials, namely, cell membrane, exosomes, proteins, peptides, lipids, fatty acids, and polysaccharides as coating materials on micron- and nano-sized particles and droplets with the functions imparted by coating with different materials. We discuss the applicability, operational parameters, and limitation of different coating techniques, from the more conventional approaches such as extrusion and sonication to the latest innovation seen on the microfluidics platform. Methods commonly used in the field to examine the coating, including its composition, physical dimension, stability, fluidity, permeability, and biological functions, are reviewed.

Hydro-thermal impacts on near-field flow and transport in a shale-hosted nuclear waste repository

Performance of geologic radioactive waste repositories depends on near-field and far-field processes, including km-scale flow and transport in engineered and natural barriers, that may require simulations of up to 1 M years of regulatory period. For a relatively short time span (less than 1000 years), the coupled thermo-hydro-mechanical–chemical (THMC) processes caused by heat from the waste package will influence near-field multiphase flow, chemical/reactive transport, and mechanical behaviors in the repository system. This study integrates the heat-driven perturbations in THM characteristics into THC simulations using PFLOTRAN to reduce dimensionality and improve computational efficiency by implementing functions of stress-dependent permeability and saturation-temperature-dependent thermal conductivity. Our reduced-order THMC simulation of a single waste package in a shale-hosted repository reveals three physical mechanisms associated with heat pulse emitted from the waste package: (1) increase of liquid mobility that accelerates the inflow and solute transport from the fully saturated host rock, (2) production of thermal gradients that results in outward flow within the buffer and disturbed rock zone (DRZ) nearest the waste package, and (3) reduction of thermal conductivity that confines heat within the engineered barrier system (EBS). These combined hydro-thermal mechanisms influence the rate of re-saturation process and transport/exchange of corrosive species in the repository system.

Experimental and numerical assessment of permeability functions in closed cavity façades

Experimental and numerical assessment of permeability functions in closed cavity façades

Sodium diffusion in heterogeneous porous media: Connecting laboratory experiments and simulations

Sodium has been suggested as a tracer for brine in reservoir formations where a significant amount of sodium ion is found mainly in the aqueous phase. Fortunately, the most abundant sodium isotope, 23Na, is Nuclear Magnetic Resonance (NMR) active, making it possible to study the structure and dynamical properties of both bulk and pore confined fluid systems. We investigated the diffusion of key dissolved sodium species in bulk solution and porous media as a function of composition, ionic strength, and permeability of the carbonate samples using NMR relaxometry and pulsed-field gradient (PFG) NMR techniques. We use Indiana limestone as an example of natural porous media and water as a freely diffusible tracer and carrier. We demonstrate apparent diffusion measurement of sodium based on changes in spin–spin relaxation time (T2) signal in pore confinement. The diffusion rate of sodium decreases with increasing counter-ion size. This effect is greater at higher ionic strengths and lower chemical potentials in porous media. The reactive transport code, CrunchFlow, was used to complement the NMR experiments to simulate diffusion behavior in porous media. Combining these two methods provides a powerful approach to estimating effective diffusion coefficients in heterogeneous matrices. The modeling considers the influence of physical properties (porosity and tortuosity) and chemical properties (geochemical composition and chemical gradients). The results presented in this work highlight the advantage of measuring apparent diffusivity using NMR T2 relaxometry in conjunction with numerical simulation to derive effective diffusivity and the corresponding matrix properties (i.e., tortuosity) of the system.

Faecalibacterium prausnitzii Attenuates CKD via Butyrate-Renal GPR43 Axis.

Despite available clinical management strategies, chronic kidney disease (CKD) is associated with severe morbidity and mortality worldwide, which beckons new solutions. Host-microbial interactions with a depletion of Faecalibacterium prausnitzii in CKD are reported. However, the mechanisms about if and how F prausnitzii can be used as a probiotic to treat CKD remains unknown. We evaluated the microbial compositions in 2 independent CKD populations for any potential probiotic. Next, we investigated if supplementation of such probiotic in a mouse CKD model can restore gut-renal homeostasis as monitored by its effects on suppression on renal inflammation, improvement in gut permeability and renal function. Last, we investigated the molecular mechanisms underlying the probiotic-induced beneficial outcomes. We observed significant depletion of Faecalibacterium in the patients with CKD in both Western (n=283) and Eastern populations (n=75). Supplementation of F prausnitzii to CKD mice reduced renal dysfunction, renal inflammation, and lowered the serum levels of various uremic toxins. These are coupled with improved gut microbial ecology and intestinal integrity. Moreover, we demonstrated that the beneficial effects in kidney induced by F prausnitzii-derived butyrate were through the GPR (G protein-coupled receptor)-43. Using a mouse CKD model, we uncovered a novel beneficial role of F prausnitzii in the restoration of renal function in CKD, which is, at least in part, attributed to the butyrate-mediated GPR-43 signaling in the kidney. Our study provides the necessary foundation to harness the therapeutic potential of F prausnitzii for ameliorating CKD.

Penetrative ferroconvection in a heterogeneous Brinkman porous medium

This study aims at finding the linear theory for the onset of ferromagnetic convective flow in a heterogeneous Brinkman porous layer with uniformly distributed internal heat source in the presence of vertical magnetic field. The resulting critical values are obtained numerically using the Galerkin technique for isothermal/insulated rigid-ferromagnetic boundaries for different forms of vertical heterogeneity permeability function [Formula: see text]. The results converge for six terms in the Galerkin expansion. The effect of types of [Formula: see text] and [Formula: see text] is found to either delay or speedup the flow of the ferrofluids. The stability of the system for the model [Formula: see text] is more stable and least stable for the model [Formula: see text] in the presence of [Formula: see text]. For different forms of [Formula: see text], the results show that the critical Rayleigh number increases with increasing [Formula: see text], while decreases with increasing [Formula: see text] and [Formula: see text]. The values of [Formula: see text] increase with [Formula: see text], but they decrease with increasing [Formula: see text] and [Formula: see text]. Besides, isothermal boundaries are found to be more stabilizing when compared to insulated boundaries.

Effects of Heavy Rainfall on Shallow Foundations in Bukit Timah Granite in Singapore

The increase in rainfall intensities due to climate change affect the entire globe. In particular, Singapore suffers from floods and rising of coastlines. Notably, in the Bukit Timah Region in Singapore, floods are getting more intense, and the region houses multitudes of low-rise constructions with shallow foundations. Damages ranging from physical, in terms of motor vehicle and property damages, to intangible losses such as major traffic delays in both private and public transit were caused by the floods. Few studies have been carried out in Singapore in terms of shallow foundations’ response to rainfall events. When rainfall infiltrates into the soil, the bearing capacity and soil stiffness are affected by the change in matric suction. Thus, the impact of heavy rainfall on shallow foundations in Bukit Timah Granite is investigated numerically using SIGMA/W. Fully coupled flow-deformation analysis with unsaturated soil characteristics, e.g., the Soil Water Characteristic Curve (SWCC) and unsaturated permeability functions, were conducted. A range of rainfall intensities, rainfall durations, and applied loadings were investigated to produce a load–settlement curve that was compared against a semi-empirical model to yield reasonable results. The studies showed that the change in matric suction is affected by the rainfall duration, rainfall intensity, initial groundwater conditions, and hydraulic properties of soil, which in turn affects the settlement response heavily. The bearing capacity is evaluated using graphical methods via the load–settlement response curves, and it was found that the reduction in matric suction heavily reduces the bearing capacity of the soil. Combined with the unsaturated residual soils and transient analyses, the discoveries give insight into the assessment of shallow foundations subjected to water infiltration.

ALOX12B and PNPLA1 Have Distinct Roles in Epidermal Lipid Lamellar Organization

The stratum corneum (SC) has remarkable permeability barrier properties that are attributable to highly ordered lipid lamellae (LL) that fill the extracellular spaces between corneocytes (Ribaud et al., 1994). Corneocytes also have a lipid monolayer covalently bound to their surfaces, called the corneocyte lipid envelope (CLE)(Crumrine et al., 2019). LL composition influences their organization and permeability function; for example, esterified omega-hydroxy (EO)-ceramides, which are synthesized by patatin-like phospholipase domain-containing 1 (PNPLA1), have been shown in vitro to mediate formation of the “long periodicity phase” triplet repeat pattern that characterizes normal LL (Opálka et al., 2016).

Ochratoxin A and Citrinin Differentially Modulate Bovine Mammary Epithelial Cell Permeability and Innate Immune Function.

Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff can be a potential threat to feed safety, animal performance and health. Ineffective biodegradation of these mycotoxins by rumen microflora following ingestion of contaminated feeds can lead to their circulatory transport to tissues such as mammary gland as the result of their biodistribution throughout the body. The bovine mammary epithelium plays a pivotal role in maintaining milk yield and composition and contributes to innate immune defense of the udder. The present study is the first to investigate individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T). Results indicated that OTA and CIT exposure for 48 h significantly decreased cell viability in a concentration-dependent manner (p < 0.05). A decrease in transepithelial electrical resistance and increase in paracellular flux of FITC-40 kDa dextran was significantly induced by OTA treatment (p < 0.05), but not by CIT after 48 h exposure. qPCR was performed for assessment of expression of tight-junction proteins, Toll-like receptor 4 (TLR4) and cytokines after 4, 24 and 48 h of exposure. Both OTA and CIT markedly downregulated expression of claudin 3 and occludin (p < 0.05), whereas CIT did not affect zonula occludens-1 expression. Expression of TLR4 was significantly upregulated by OTA (p < 0.001) but downregulated by CIT (p < 0.05) at 48 h. Expression of IL-6, TNF-a and TGF-β was significantly upregulated by OTA (p < 0.05), whereas IL-6 and TGF-β expression was downregulated by CIT (p < 0.01). These results suggest that OTA and CIT could potentially differentially modulate barrier and innate immune functions of mammary epithelium. The present study not only throws light on the individual toxicity of each mycotoxin on bovine mammary epithelium but also lays the foundation for future studies on the combined effects of the two mycotoxins.

Experimental characterization of {text {H}}_2/water multiphase flow in heterogeneous sandstone rock at the core scale relevant for underground hydrogen storage (UHS)

Geological porous reservoirs provide the volume capacity needed for large scale underground hydrogen storage (UHS). To effectively exploit these reservoirs for UHS, it is crucial to characterize the hydrogen transport properties inside porous rocks. In this work, for the first time in the community, we have performed {text {H}}_2/water multiphase flow experiments at core scale under medical X-ray CT scanner. This has allowed us to directly image the complex transport properties of {text {H}}_2 when it is injected or retracted from the porous rock. The important effective functions of capillary pressure and relative permeability are also measured, for both drainage and imbibition. The capillary pressure measurements are combined with MICP data to derive a receding contact angle for the {text {H}}_2/water/sandstone rock system. The rock core sample is a heterogeneous Berea sandstone (17 cm long and 3.8 cm diameter). Our investigation reveals the interplay between gravitational, capillary, and viscous forces. More specifically, it illustrates complex displacement patterns in the rock, including gravity segregation, enhancement of spreading of {text {H}}_2 due to capillary barriers, and the formation of fingers/channel during imbibition which lead to significant trapping of hydrogen. These findings shed new light on our fundamental understanding of the transport characteristics of {text {H}}_2/water relevant for UHS.

The Influence of Movable Water on the Gas-Phase Threshold Pressure Gradient in Tight Gas Reservoirs

Threshold pressure gradient (TPG) is a key parameter determining the pore-scale fluid dynamics. In tight gas reservoirs, both gas and water exist in the porous rock, and the existing water can be divided into irreducible and movable water. However, how movable water saturation will influence TPG has not yet been investigated. Therefore herein, nuclear magnetic resonance (NMR) and high-pressure mercury intrusion (HPMI) experiments were performed to determine pore-scale water distribution, movable water saturation, and pore throat distribution in the core plugs. Subsequently, the air bubble method was used to measure TPG as a function of movable water saturation and permeability inside tight gas core plugs, finding that TPG increased from 0.01 MPa/m to 0.25 MPa/m with the movable saturation increased from 2% to 35%. Finally, a semi-empirical model was derived to describe the correlation between TPG, movable water saturation, and permeability, which performed better than previous models in the literature. These insights will advance the fundamental understanding of TPG in tight gas reservoirs and provide useful guidance on tight gas reservoirs development.

Numerical investigation on light non–aqueous phase liquid flow in the vadose zone considering porosity effects on soil hydraulic properties

AbstractIn the management of light non–aqueous phase liquid (LNAPL)‐contaminated ground, numerical simulation is widely used to analyze LNAPL flow in the unsaturated soil (vadose) zone. Porosity effects on the hydraulic properties of unsaturated soils are highly simplified in existing mathematical models. Some important features, such as the nonlinear relation between porosity and permeability/displacement pressure, cannot be well captured. To address this problem, a new mathematical model was developed in this study, considering porosity effects on hydraulic properties of soils, including the retention behavior and permeability function of LNAPL and water. The newly developed model was implemented in MATLAB using the finite difference method and then verified by the results of a centrifuge test. Then, parametric studies were conducted to investigate the flow of LNAPL upon an active leakage at the ground surface. Based on the computed results, the influence of several factors, such as porosity magnitude, porosity distribution, and soil layering, was revealed. In particular, an increase in the porosity leads to a significant increase in the volume of LNAPL leaked into the ground, the vertical front depth, and the area of contaminated ground. This is mainly because the porosity affects not only the intrinsic permeability but also the relative permeability because (a) the intrinsic permeability of soils is larger at a higher porosity (b) when the porosity is higher, the equilibrium water saturation at a given capillary pressure is smaller. Consequently, LNAPL can achieve a larger degree of saturation and higher relative permeability.

Preparation of biological monolayers for producing high-resolution scanning electron micrographs.

Scanning electron microscopy (SEM) provides a technical platform for nanoscopic mapping of biological structures. Correct preparation of SEM samples can provide an unprecedented understanding of the nexus between cellular morphology and topography. This comparative study critically examines two coating methods for preparing biological samples for scanning electron microscopy, while also providing novel advice on how to prepare in vitro epithelial or endothelial samples for high-resolution scanning-electron microscopy (HR-SEM). Two obstacles often confront the biologist when investigating cellular structures grown under tissue culture conditions, namely., how to prepare and present the biological samples to the HR-SEM microscope without affecting topographical membrane and cellular structural alterations. Firstly, our use of the Millicell cellulose inserts on which to grow our cellular samples in preparation for HR-SEM is both novel and advantageous to comparing the permeability function of cells to their morphological function. Secondly, biological material is often non-conducting, thermally sensitive and fragile and, therefore, needs to be fixed correctly and coated with thin conducting metal to ensure high-resolution detail of samples. Immortalized mouse brain endothelial cells (bEnd5) was used as a basis for describing the preferences in the use of the protocol. We compare two biological sample coating modalities for the visualizing and analysis of texturized, topographical, membranous ultrastructures of brain endothelial cell (BEC) confluent monolayers, namely, carbon and gold:palladium (Au:Pd) sputter coating in preparation for HR-SEM. BEC monolayers sputter-coated with these two modalities produced three-dimensional micrographs which have distinctly different topographical detail from which the nanostructural cellular data can be examined. The two coating methods display differences in the amount of nanoscopic detail that could be resolved in the nanosized membrane cytoarchitecture of BEC monolayers. The micrographical data clearly showed that Au:Pd sputter-coated samples generate descript imagery, providing useful information for profiling membrane nanostructures compared to carbon-coated samples. The recommendations regarding the contrast in two modalities would provide the necessary guidance to biological microscopists in preparing tissue culture samples for HR-SEM.

A dual-continuum model (TOUGH2) for characterizing flow and discharge in a mechanically disrupted sandstone overburden

Underground hard coal mining usually disrupts the mechanical equilibrium of rock sequences, creating fractures within minor permeable rocks. The present study employs a dual-continuum model to assess how both fractured and porous sandstone media influence the percolation process in postmining setups. To test the approach, the software TOUGH2 was employed to simulate laminar fluid flow in the unsaturated zone of the Ibbenbüren Westfield mining area. Compared to other coal mining districts in Germany, this area is delineated by the topography and local geology, leading to a well-defined hydrogeological framework. Results reveal good agreement between the calculated and measured mine water discharge for the years 2008 and 2017. The constructed model was capable of reproducing the bimodal flow behavior of the adit by coupling a permeable fractured continuum with a low-conductivity rock matrix. While flow from the fractured continuum results in intense discharge events during winter months, the rock matrix determines a smooth discharge limb in summer. The study also evaluates the influence of individual and combined model parameters affecting the simulated curve. A detailed sensitivity analysis displayed the absolute and relative permeability function parameters of both continua among the most susceptible variables. However, a strong a priori knowledge of the value ranges for the matrix continuum helps to reduce the model ambiguity. This allowed for calibration of some of the fractured medium parameters for which sparse or variable data were available. However, the inclusion of the transport component and acquisition of more site-specific data is recommended to reduce their uncertainty.

Lactose hydrate can increase the transcellular permeability of β-naphthol in rat jejunum and ileum.

The unstirred water layer (UWL) is an integral part of the apical surface of mucosal epithelia and comprises mucins (MUC), for which there are many molecular species. Galectins, a family of β-galactoside-bindinglectins, form a lattice barrier on surface epithelial cells by interacting with MUC. Lactose inhibits the galectin-MUC interaction. Therefore, the present study investigated the galectin-MUC interaction in the mucosa of the gastrointestinal tract and its role in intestinal barrier functions. The effects of lactose hydrate (LH) on the membrane permeability of the rat small intestine and Caco-2 cells were examined. LH enhanced the membrane permeability of the rat small intestine, which contains the UWL, via a transcellular route, for which the UWL is the rate limiting factor. The membrane permeability of Caco-2 cells, in which the UWL is insufficient, was not affected by LH. The apparent permeability coefficient (Papp) of a paracellular marker was not significantly altered in the rat small intestine or Caco-2 cells treated with LH at any concentration. Furthermore, the Papp of β-naphthol which is a transcellular marker was not significantly altered in Caco-2 cells treated with LH, but was significantly increased in the rat small intestine in a LH concentration-dependent manner. The present results demonstrate that the physical barrier has an important function in gastrointestinal membrane permeability, and LH-induced changes increase the transcellular permeability of β-naphthol in rat small intestine.

Blood brain barrier permeability and immune function of brain in rainbow trout responding to IHNV infection

Viral infection of the central nervous system (CNS) is often associated with blood-brain barrier (BBB) disruption. Mammals have developed complicated and efficient immune strategies to protect the BBB. However, the immune defense of brain and BBB permeability changes are not well-understood in teleost during virus invading. In this study, we constructed an infectious hematopoietic necrosis virus (IHNV) immersion infected rainbow trout model. After IHNV infection, pathological changes occurred in the brain, and MPO and ROS activities were significantly increased. In addition, the expression levels of BBB permeability-related genes were also changed. Transcriptome analysis showed that immune-related genes and signaling pathways in the brain were activated after IHNV infection. These results showed that the permeability of BBB increased significantly after IHNV infection, thus activating immune related factors and cells to enter the CNS through blood circulation to resist pathogenic infection.

Dietary Moringa oleifera leaf powder improves jejunal permeability and digestive function by modulating the microbiota composition and mucosal immunity in heat stressed rabbits.

Heat stress (HS) has detrimental effects on intestinal health by altering digestive and immune responses in animals. Dietary Moringa oleifera leaf powder (MOLP) has been implicated in ameliorating the impact of HS, but its effects in terms of intestinal function improvement under HS remain poorly characterized. Therefore, the current study investigated the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs) in jejunal mucosa of heat-stressed rabbits by using the next-generation sequencing techniques. A total of 21 male New Zealand White rabbits (32weeks old mean body weight of 3318 ± 171g) were divided into three groups (n = 7/group) as control (CON, 25°C), heat stress (HS, 35°C for 7h daily), and HS with MOLP supplementation (HSM, 35°C for 7h daily) gavage at 200mg/kg body weight per day for 4weeks. The results indicated that MOLP supplementation increased mRNA expression of tight junction proteins and glutathione transferase activity, while the malonaldehyde concentration was decreased in the jejunal mucosa compared to HS group (P < 0.05). Furthermore, MOLP decreased the concentrations of lipopolysaccharide, pro-inflammatory cytokines, and myeloperoxidase compared with HS group (P < 0.05). Intestinal microbiota analysis revealed that at phyla level, the relative abundance of Bacteroidetes was higher in HSM group compared to CON and HS groups. MOLP supplementation also resulted in higher abundance of putatively health-associated genera such as Christensenellaceae R-7 gut group, Ruminococcaceae NK4A214 group, Ruminococcus 2, Lachnospiraceae NK4A136 group, and Lachnospiraceae unclassified along with higher butyrate levels in HSM group as compared to HS group. The analysis of DEGs revealed that MOLP reversed inflammatory response by downregulation of genes, such as TNFRSF13C, LBP, and COX2 in enriched KEGG pathway of NF-kβ pathway. MOLP supplementation also significantly upregulated the expression of genes in protein digestion and absorption pathway, including PRSS2, LOC100349163, CPA1, CPB1, SLC9A3, SLC1A1, and SLC7A9 in HSM group. Three genes of fibrillar collagens, i.e., COL3A1, COL5A3, and COL12A1 in protein digestion were also down-regulated in HSM group. In conclusion, MOLP supplementation could improve jejunal permeability and digestive function, positively modulate microbiota composition and mucosal immunity in heat-stressed rabbits.

Inductance Analysis of Two-Phase Winding Segmented Permanent Magnet Linear Synchronous Motor

The inductance of a winding segmented permanent magnet linear synchronous motor (WS-PMLSM) is affected by winding disconnection and coupling length variation, which makes the variation of inductance more complicated, and this paper proposes incremental inductance, apparent inductance, and positional inductance to reveal this phenomenon, which gives a theoretical basis for mathematical modeling and thrust fluctuation suppression. First, an analytical approach is used to derive a fully coupled state model using the magnetomotive force and specific permeability function. Second, the domain of the specific permeability function is extended and the inductance expressions are calculated for the whole moving range. Finally, the inductance of the prototype WS-PMLSM with a two-phase winding is experimentally verified, and it is proposed that the effects of the three inductive components on the system should be considered comprehensively when implementing control of the WS-PMLSM.