Objective: Chronic ankle instability (CAI) leads to a decrease in physical performance and a delay in return to sportive activities in elite athletes. Approximately 40% of the lateral ankle sprains result in CAI. The purpose of the current study was to compare the functionality of foot and ankle and health-related quality of life in elite athletes with or without CAI. Material and Methods: A total of 104 elite athletes 79 men, 25 women with (n=52) and without (n=52) CAI participated in this study. The functionality of the foot and ankle was determined using Foot and Ankle Ability Measure (FAAM) FAAMactivity of daily living and FAAM-sports subscales. Health-related quality of life was assessed using the short form-12 (SF-12) physical and mental subscores. Results: FAAM-activity of daily living and FAAM-sport subscale scores of elite athletes with CAI were lower than athletes without CAI (p<0.05). The SF-12 physical and mental subscores differed between the 2 groups (p<0.05) and the most affected side was the dominant one in chronic ankle insatbility group. Conclusion: CAI causes functional performance decline and mental impairment by showing general and regional symptoms in the Professional athletic population. During the planning of the treatment and rehabilitation process, priority should be given to increase the perceived quality of life by diminishing the symptoms. In addition, focusing on multidimensional treatment approaches that take into account the level of mental health would be beneficial in increasing athletic performance.