Functional Microorganisms Research Articles

Root architectures differentiate the composition of organic carbon in bauxite residue during natural vegetation

Understanding plant root architectures induced changes in organic carbon accumulation and conversion is critical to predicting carbon cycling and screening appropriate plant species for ecological restoration on bauxite residue disposal areas. According to the ecological investigation of a weathered bauxite residue disposal area, three plants with different root architectures including Artemisia lavandulaefolia (A. lavandulaefolia), moss, and Zanthoxylum simulans (Z. simulans) were selected to investigate the rhizosphere effects on the composition and structure of organic carbon in bauxite residue. The physic-chemical properties, the contents and structure of different organic carbon fractions, and microbial communities of bauxite residue from rhizosphere and non-rhizosphere were analyzed. Plant growth decreased the saline-alkalinity, increased the contents of total organic carbon, particulate organic carbon and dissolved organic carbon, whilst enhancing the enzymatic activities of bauxite residue. Meanwhile, the rhizosphere effects had significant effects on the accumulation and stabilization of organic carbon in bauxite residue. A. lavandulaefolia had the strongest rhizosphere effects on the composition and structure of total organic carbon and dissolved organic carbon, whilst moss was more effective on the accumulation of particulate organic carbon in bauxite residue. Plant growth and root architecture changed the abundance of specific functional microorganisms and the complexity of microbial co-occurrence networks, thus elevating organic carbon levels in bauxite residue. During natural vegetation encroachment, rhizosphere exciting effects of the salt-tolerated plants could change the composition and structure of organic carbon fractions due to the comprehensive effectiveness of the improvement of physic-chemical properties and microbial communities. The findings improve our understanding of the responses of sequestration and stabilization of organic carbon pools to ecological restoration on bauxite residue disposal areas.

Enhancement of nitrogen removal in coupling Anammox and DAMO via Fe-modified granular activated carbon

Anaerobic ammonium oxidation (Anammox) coupled with Denitrifying anaerobic methane oxidation (DAMO) is an attractive technology to simultaneously remove nitrogen and mitigate methane emissions from wastewater. However, its nitrogen removal rate is usually limited due to the low methane mass transfer efficiency, low metabolic activity and slow growth rate of functional microorganisms. In this study, GAC and Fe-modified GAC (Fe-GAC) were added into Anammox-DAMO process to investigate their effects on nitrogen removal rates and then reveal the mechanism. The results showed that after 80-day experiments, the total nitrogen removal rate was slightly improved in the presence of GAC (3.94 mg L−1·d−1), while it reached high as 16.66 mg L−1·d−1 in the presence of Fe-GAC, which was ca.17 times that of non-amended control group (0.96 mg L−1·d−1). The addition of Fe-GAC stimulated the secretion of extracellular polymeric substance (EPS), improved the electron transfer capability and promoted the production of Cytochrome C. Besides, the key functional enzyme activities (HZS, HDH and NAR) were highest in the Fe-GAC group, which were approximately 1.06–1.56 times higher than those of GAC-amended and blank control groups. Microbial community analysis showed that the abundance of the DAMO archaea (Candidatus Methanoperedens) and Anammox bacteria (Candidatus Brocadia) were remarkably increased with the addition of Fe-GAC. Functional genes associated with nitrogen removal and methane oxidation in Fe-GAC system were up-regulated. This study provides a promising strategy for achieving high rate of nitrogen removal upon Anammox-DAMO process.

Performance of a mixed inoculum of sludge and pit mud for short and medium-chain fatty acids production: Insight into key microbiome and functional potential in anaerobic fermentation inoculum

In this study, medium-chain fatty acids (MCFA) production from food waste with ethanol as electron donor using three different inoculants (active sludge, pit mud and mixture of sludge and pit mud). The results showed that the R3 (mixed inoculum of sludge and pit mud) produced 8539.1 mg COD/L of MCFA, which was significantly higher than that produced by R1 (active sludge, 2712.6 mg COD/L) and R2 (pit mud, 3585.9 mg COD/L). The major MCFA was caproate and heptanoate, and the short-chain fatty acids (SCFA) and MCFA selectivity reaches 69.2%. Microbial community analysis revealed that the relative abundance levels of Clostridium_sensu_stricto_1, Clostridium_sensu_stricto_12 and Oscibacter were positively correlated with MCFA production. Further analysis of the enzymes related genes involved in the MCFA biosynthesis indicates that they were mainly related to enzymes of Clostridium, Oscibacter and Ruminiclostridium. These were the key genera involved in the chain elongation process. Metagenomic analysis revealed that fatty acid biosynthesis (FAB) pathway was the main pathway of MCFA synthesis in all three fermentation reactors. The abundance levels of all FAB pathway related-genes and MCFA functional microorganisms were higher in R3. Additionally, the enrichment of the main gene abundance of metabolic pathway revealing that the mixed inoculum of sludge and pit mud combined the ability of fatty acid biosynthesis and carbohydrate metabolism, with higher fermentation potential. Overall, this study provides new strategies and insights for MCFA production.

Effect of different moisture contents on hydrogen sulfide malodorous gas emission during composting

The sulfate reduction reaction releases malodorous gases (H2S) during composting, with potential pollution risks to the environment. In this study, chicken manure (CM) with high sulfur content and beef cattle manure (BM) with low sulfur content were used to investigate the effect of control (CK) and low moisture content (LW) on sulfur metabolism. The results showed that compared to CK composting, the cumulative H2S emission of CM and BM composting decreased by 27.27% and 21.08% under LW condition, respectively. Meanwhile, the abundance of core microorganisms related to sulfur components was reduced under LW condition. Furthermore, the KEGG sulfur pathway and network analysis suggested that LW composting weakened the sulfate reduction pathway, and reduced the number and abundance of functional microorganisms and genes. These results indicated that low moisture content had important effects on inhibiting the release of H2S during composting, which provided a scientific basis to control environmental pollution.

Enhanced aquaculture wastewater treatment in a biofilm reactor filled with sponge/ferrous oxalate/biochar composite (Sponge-C2FeO4@NBC) biocarriers: Performance and mechanism

Fabricating low-cost and efficient biofilm carriers for moving bed biofilm reactors in wastewater treatment is crucial for achieving environmental sustainability. Herein, a novel sponge biocarrier doped with NaOH-loaded biochar and nano ferrous oxalate (sponge-C2FeO4@NBC) was prepared and evaluated for nitrogenous compounds removal from recirculating aquaculture systems (RAS) wastewater by stepwise increasing ammonium nitrogen (NH4+-N) loading rates. The prepared NBC, sponge-C2FeO4@NBC, and matured biofilms were characterized using SEM, FTIR, BET, and N2 adsorption-desorption techniques. The results reveal that the highest removal rates of NH4+-N reached 99.28 ± 1.3% was yielded by the bioreactor filled with sponge-C2FeO4@NBC, with no obvious nitrite (NO2−-N) accumulation in the final phase. The reactor packed with sponge-C2FeO4@NBC biocarrier had the highest relative abundance of functional microorganisms responsible for nitrogen metabolism than in the control reactor, confirmed from 16S rRNA gene sequencing analysis. Our study provides new insights into the newly developed biocarriers for enhancing RAS biofilters treatment performance in keeping water quality within the acceptable level for the rearing of aquatic species.

Characterization of Functional Microorganisms in Representative Traditional Fermented Dongcai from Different Regions of China.

Dongcai is loved for its delicious flavor and nutritional value. The microorganisms in Dongcai play a vital role in their flavor, quality, and safety, and the microbial communities of Dongcai vary greatly from region to region. However, it remains unknown what the predominant microorganisms are in different traditional Dongcai and how they affect its flavor. The objective of this study is to explore the microbial diversity of traditional fermented Dongcai in three representative Chinese regions (Tianjin, Sichuan, and Guangzhou) and further assess their microbial functions. The microbial diversity of fermented Dongcai in Guangdong has the lowest diversity compared to fermented Dongcai in Sichuan, which has the highest. The distribution of the main genera of fermented Dongcai varies from region to region, but Carnimonas, Staphylococcus, Pseudomonas, Sphingomonas, Burkholderia-Caballeronia-Paraburkholderia, and Rhodococcus are the dominant genera in common. In addition, halophilic bacteria (HAB, i.e., Halomonas Bacillus, Virgibacillus, etc.) and lactic acid bacteria (LAB, i.e., Weissella and Lactobacillus) are also highly abundant. Of these, Burkholderia-Caballeronia-Paraburkholderia, Rhodococcus, Sphingomonas, Ralstonia, and Chromohalobacter are dominant in the Sichuan samples. In the Tianjin samples, Lactobacillus, Weissella, Virgibacillus, Enterobacter, Klebsiella, and Pseudomonas are the most abundant. Predictions of microbial metabolic function reveal that carbohydrates, amino acids, polyketides, lipids, and other secondary metabolites are abundantly available for biosynthesis. In addition, the different flavors of the three types of Dongcai may be due to the fact that the abundance of HAB and LAB shows a significant positive correlation with the amounts of important metabolites (e.g., salt, acid, amino nitrogen, and sugar). These results contribute to our understanding of the link between the distinctive flavors of different types of Dongcai and the microorganisms they contain and will also provide a reference for the relationship between microbial communities and flavor substances in semi-fermented pickles.

Long-term in-situ starvation and reactivation of co-digestion with food waste and corn straw in a continuous AnDMBR: Performance, sludge characteristics, and microorganism community

To explore the effects of in-situ starvation and reactivation in a continuous anaerobic dynamic membrane reactor (AnDMBR), the anaerobic co-digestion system of food waste and corn straw was firstly start-up and stability operated, and then stopped feeding substrate approximately 70 days. After long-term in-situ starvation, the continuous AnDMBR was reactivated using the same operation conditions and organic loading rate as the continuous AnDMBR used before in-situ starvation. Results shown that the anaerobic co-digestion of corn straw and food waste in the continuous AnDMBR can resume stable operation within 5 days, and the corresponding methane production of 1.38 ± 0.26 L/L/d was completely returned to the methane production before in-situ starvation (1.32 ± 0.10 L/L/d). Through analysis of the specific methanogenic activity and key enzyme activity of the digestate sludge, only the acetic acid degradation activity of methanogenic archaea can be partially recovered, however, the activities of lignocellulose enzyme (lignin peroxidase, laccase, and endoglucanase), hydrolase (α-glucosidase) and acidogenic enzyme (acetate kinas, butyrate kinase, and CoA-transferase) can be fully recovered. Analysis of microorganism community structure using metagenomic sequencing technology showed that starvation decreased the abundance of hydrolytic bacteria (Bacteroidetes and Firmicutes) and increased the abundance of small molecule-utilizing bacteria (Proteobacteria and Chloroflexi) due to lack of substrate during the long-term in-situ starvation stage. Furthermore, the microbial community structure and key functional microorganism still maintained and similar with that of starvation final stage even after long-term continuous reactivation. The reactor performance and sludge enzymes activity in the continuous AnDMBR co-digestion of food waste and corn straw can be well reactivated after long-term in-situ starvation, even though the microbial community structure can not be recovered to the initiating stage.

The positive effects of mineral-solubilizing microbial inoculants on asymbiotic nitrogen fixation of abandoned mine soils are driven by keystone phylotype

Toward the restoration of the increasing numbers of abandoned mines across China, external-soil spray seeding technologies have become more extensively utilized. However, considerable challenges remain that seriously hamper the effectiveness of these technologies, such as inadequate nutrient availability for plants. Previous studies have shown that mineral-solubilizing microbial inoculants can increase the nodules of legumes. However, their effects on symbiotic nitrogen fixation (SNF), asymbiotic nitrogen fixation (ANF), and diazotrophic communities remain unknown. Further, research into the application of functional microorganisms for the restoration of abandoned mines has been conducted either in greenhouses, or their application in the field has been too brief. Thus, we established a four-year field experiment in an abandoned mine and quantified the SNF, ANF, and diazotrophic communities. To the best of our knowledge, this study is the first to describe the long-term application of specific functional microorganisms for the remediation of abandoned mine sites in the field. We revealed that mineral-solubilizing microbial inoculants significantly increased the soil ANF rate and SNF content. There was no significant correlation between the diazotrophic alpha diversity and soil ANF rate; however, there were strong positive associations between the relative abundance and biodiversity of keystone phylotype (module #5) within ecological clusters and the ANF rate. Molecular ecological networks indicated that microbial inoculants increased network complexity and stability. Moreover, the inoculants significantly enhanced the deterministic ratio of diazotrophic communities. Furthermore, homogeneous selection predominantly mediated the assembly of soil diazotrophic communities. It was concluded that mineral-solubilizing microorganisms played a critical role in maintaining and enhancing nitrogen, which offers a new solution with great potential for the restoration of ecosystems at abandoned mine sites.

Sustained anaerobic degradation of 4-chloro-2-methylphenoxyacetic acid by acclimated sludge in a continuous-flow reactor

4-Chloro-2-methylphenoxyacetic acid (MCPA) is a widely used herbicide across the world. MCPA is persistent and easily transports into anoxic environment, such as groundwater, sediments and deep soils. However, little research on anaerobic microbial degradation of MCPA was carried out. The functional microorganisms as well as the catabolic pathway are still unknown. In this research, an anaerobic MCPA-degrading bacterial consortium was enriched from the river sediment near a pesticide-manufacturing plant. After about 6 months’ acclimation, the MCPA transformation rate of the consortium reached 4.32 μmol g−1 day−1, 25 times faster than that of the original sludge. 96% of added MCPA (2.5 mM) was degraded within 9 d of incubation. Three metabolites including 4-chloro-2-methylphenol (MCP), 2-methylphenol (2-MP) and phenol were identified during the anaerobic degradation of MCPA. An anaerobic catabolic pathway was firstly proposed: firstly, MCPA was transformed to MCP via the cleavage of the aryl ether, then MCP was reductively dechlorinated to 2-MP which was further demethylated to phenol. The 16S rRNA gene amplicon sequencing revealed a substantial shift in the bacterial community composition after the acclimation. SBR1031, Acidaminococcaceae, Aminicenantales, Syntrophorhabdus, Acidaminobacter, Bacteroidetes_vadinHA17, Methanosaeta, Bathyarchaeia, KD4-96, Anaeromyxobacter, and Dehalobacter were significantly increased in the enriched consortium after acclimation, and positively correlated with the anaerobic degradation of MCPA as suggested by heat map correlation analysis. This study provides a basis for further elucidation of the anaerobic catabolism of MCPA, and contributes to developing efficient and low-cost anaerobic treatment technologies for MCPA pollution.

Municipal Sewage Treatment Technology: A2/O-VMBR Integrated Technology for Municipal Treatment and Improved Pollutant Removal

To modernize wastewater treatment plants, a pilot-scale anaerobic/anoxic/oxic-vibrating membrane bioreactor (A2/O-VMBR) was developed and successfully operated. Despite a low C/N ratio, the A2/O-VMBR achieved removal rates of 61.10%, 93.77%, 72.86%, and 54.75% for COD, TN, TP, and NH3-N, respectively. The maximum and extremity transmembrane pressures were 45 kPa, and 80 kPa, respectively, with no sludge bulking observed. The VMBR saved over 96–98% of energy compared to traditional MBR plants, making it a better option for municipal wastewater treatment. High-throughput sequencing analysis revealed identical bacterial population structures in samples obtained from the treatment units, with genera having nitrifying, denitrifying, hydrolyzing, and glycogen-accumulating activities, which allowed for nitrogen removal. The key functional microorganisms responsible for nitrification–denitrification were species belonging to the genera FCPU426, Fusobacteria, Planctomycetes, Verrucomicrobia, and Epsilonbacteraeota. The integrated experimental system produced favorable results in improving wastewater quality, highlighting the usability of the A2/O-VMBR technology. Therefore, this technique holds potential for further investigation into the context of wastewater treatment and recovery.

Microbial succession and its effect on the formation of umami peptides during sufu fermentation.

Sufu, a traditional Chinese fermented food, is famous for its unique flavor, especially umami. However, the formation mechanism of its umami peptides is still unclear. Here, we investigated the dynamic change of both umami peptides and microbial communities during sufu production. Based on peptidomic analysis, 9081 key differential peptides were identified, which mainly involved in amino acid transport and metabolism, peptidase activity and hydrolase activity. Twenty-six high-quality umami peptides with ascending trend were recognized by machine learning methods and Fuzzy c-means clustering. Then, through correlation analysis, five bacterial species (Enterococcus italicus, Leuconostoc citreum, L. mesenteroides, L. pseudomesenteroides, Tetragenococcus halophilus) and two fungi species (Cladosporium colombiae, Hannaella oryzae) were identified to be the core functional microorganisms for umami peptides formation. Functional annotation of five lactic acid bacteria indicated their important functions to be carbohydrate metabolism, amino acid metabolism and nucleotide metabolism, which proved their umami peptides production ability. Overall, our results enhanced the understanding of microbial communities and the formation mechanism of umami peptides in sufu, providing novel insights for quality control and flavor improvement of tofu products.

Enrichment and response of iron-metabolizing microorganisms and metabolic genes in the contaminated area of stratified stacking coal gangue dumps, Northern China.

In the Xishan coalfield of northern China, the stratified stacking of soil and gangue was applied to limit the acid pollution from high-sulfur coal gangue. In this study, we found that stratified stacking can effectively neutralize the acidity, with the pH value of gangue-leaching water being 6.02-8.13. In contrast to the acidic contaminated area, most of the microorganisms in the study area sediment were neutrophilic, with the main genera being Arthrobacter, Pseudorhodobacter, Pseudomonas, and Rhodoferax. A variety of iron- and sulfur-metabolizing bacteria was discovered in the gangue-leaching sediment, with the total relative abundance ranging from 4.20 to 23.75%, of which the iron-reducing bacteria (FeRB) accounted for the highest percentage. The distributions of these functional microorganisms in the samples were significantly influenced by Fe and S. The co-occurrence network analysis revealed a significant positive correlation between the iron- and sulfur-metabolizing bacteria in the sediment (93.75%), indicating a strong reciprocal symbiotic relationship between these bacteria. The iron and sulfur metabolism genes in the sediment were predicted and compared based on the Tax4Fun functional prediction method. Results showed that functional genes related to iron metabolism were highly expressed in the gangue-leaching sediment. This study enhances the understanding of iron and sulfur metabolism in gangue-leaching contaminated areas.

Effects of two plant species combined with slag-sponges on the treatment performance of contaminated saline water in constructed wetland

Constructed wetland (CW), an ecological water treatment system, can purify and repair the damaged saline water body in an open watershed, but its repairing function is limited at low temperature under salt stress. In this study, two different plant species with slag-sponge layer were operated to enhance the purification effect of CW on the damaged saline water body. The results showed that the combination of Scirpus mariqueter and slag-sponges in CW had a better purification effect especially under the condition of salinity of 10‰ (S = 10) with a respective removal efficiency of 91.04% of total nitrogen, 80.07% of total phosphorus, and 93.02% of COD in high temperature (25 ~ 35°C). Furthermore, ecological traits (enzyme activity and amino acids) of plants, the abundance and distribution of functional microorganisms on the surface of slag-sponges, and the microbial state on the substrate surface of the denitrifying zone of CW were analyzed to explain how exactly the combinations worked. It was found that the enrichment of functional microorganisms in slag-sponge and the anaerobic zone of plants have improved the nitrogen and phosphorus removal. Plants maintained high enzyme activities and the ability to synthesize key amino acids under salt stress to ensure the growth and reproduction of plants and achieve the assimilation function. Scirpus mariqueter combined with slag-sponges in CW effectively improved the purification effect of damaged saline water, indicating that it is an ecological and green saline water treatment way.

Temperature and pH on microbial desulfurization of sulfide wastewater: From removal performance to gene regulation mechanism

Sulfides are widely present in industrial wastewater cause environmental problems like malodorous, corrosive, and toxic. Microbial desulfurization technology has the advantages of ambient conditions, low energy consumption, and enables the recovery of sulfur (S0). These advantages make it the technology of choice for treating middle and low loads of sulfide wastewater. Temperature and pH are two important factors that affect the product selectivity of microbial desulfurization, but the mechanism of these two factors regulating the genes and metabolic pathways of functional microorganisms remains unclear. In this study, a sequencing batch reactor (SBR) treated sulfide-containing wastewater, achieving complete degradation of 400 mg/L influent sulfide at 30 °C and pH 7.5 and showed the best S0 formation efficiency (91.1 ± 2.1 %). The Halothiobacillus genus dominated the microbial community, with the highest abundance (73.0 %) at 30 °C and pH 7.5. Metagenomic analysis showed that at 30 °C and pH 7.5 compared with 20 °C or pH 6.5, the abundance of sulfide oxidation genes (sqr, fccAB) were significantly increased (49.9 % and 43.0 %, 1303.3 % and 139.4 %). Under unfavorable temperature and pH, fccAB gene abundance decreased significantly (92.9 %, 58.2 %), sqr decreased less (33.3 %, 30.1 %), and sox increased significantly (241.0 %, 122.1 %). The dominate sulfide oxidation pathway from flavocytochrome c (FCC) shifted to the sulfide: quinone oxidoreductase (SQR) and sulfur oxidizing system (SOX) pathways. This work highlights the ability of modulating temperature and pH to optimize the biological treatment of sulfide-containing wastewater processes and obtaining more S0, and elucidates their effects on metabolic pathways associated with sulfide oxidation.

Effects of AMF Compound Inoculants on Growth, Ion Homeostasis, and Salt Tolerance-Related Gene Expression in Oryza sativa L. Under Salt Treatments

Increased soil salinization is among the main factors that limits safe rice production. Arbuscular mycorrhizal fungi (AMF) have been shown to alleviate the toxic effects of salt stress in plants. However, more studies on AMF combined with other functional microorganisms are needed to further improve salt tolerance in rice. Therefore, the compound inoculum Funneliformis mosseae (Fm) together with two functional microorganisms, Piriformospora indica (Pi) and Agrobacterium rhizogenes (Ar) was evaluated for their effect on the rice growth, photosynthetic gas exchange parameters, ion homeostasis, and the expression of salt tolerance-related genes under 0, 80, 120 and 160 mM salt stress conditions. The results showed that: (1) the rice seedling biomass of the AMF compound inoculant treatment group was significantly higher than that of the non-inoculation treatment group (P < 0.05); (2) under NaCl stress, inoculation with AMF compound inoculants can activate the rice antioxidant enzyme system and improve osmoregulation ability; (3) AMF compound inoculants can increase the concentration of K+ in the plant and inhibit the transfer of Na+ to rice leaves, maintaining a high K+/Na+; and (4) AMF compound inoculants could induce and regulate the overexpression of genes related to salt tolerance, photosynthesis and ion homeostasis in rice, and improve the tolerance of rice under salt stress. Our study showed that AMF compound inoculants could improve the adaptability of rice under NaCl stress and promote plant growth by regulating the photosynthetic gas exchange parameter, reactive oxygen species (ROS) scavenging ability, and ion homeostasis of plants. These results suggest that AMF compound inoculants may play an important role in improving rice productivity in salinized soil.Graphical

Effect of Multiple Rounds of Enrichment on Metabolite Accumulation and Microbiota Composition of Pit Mud for Baijiu Fermentation.

Pit mud (PM) is the main component of Baijiu (traditional Chinese liquor), and its microorganisms are the primary sources of the aroma of Chinese strong-flavor Baijiu (SFB). Enrichment plays an important role in the selection of functional microorganisms in PM. Herein, the PM of SFB was submitted to six rounds of enrichment using clostridial growth medium (CGM), and changes in the metabolite accumulation and microbiota composition were evaluated. Based on the metabolite production and microbiota composition, the enrichment rounds were classified as the acclimation stage (round 2), main fermentation stage (rounds 3 and 4), and late fermentation stage (rounds 5 and 6). Species within the genus Clostridium dominated in the acclimation stage (65.84-74.51%). In the main fermentation stage, the dominant microbial groups were producers of butyric acid, acetic acid, and caproic acid, which included Clostridium (45.99-74.80%), Caproicibacter (1.45-17.02%), and potential new species within the order of Oscillataceae (14.26-29.10%). In the late stage of enrichment, Pediococcus dominated (45.96-79.44%). Thus, the main fermentation stage can be considered optimal for the isolation of acid-producing bacteria from PM. The findings discussed herein support the development and application of functional bacteria by bioaugmentation, and contribute to improving the quality of PM and SFB production.

Polyethylene and polyvinyl chloride microplastics promote soil nitrification and alter the composition of key nitrogen functional bacterial groups

Microplastics (MPs) contamination in soils seriously threatens agroecosystems globally. However, very few studies have been done on the effects of MPs on the soil nitrogen cycle and related functional microorganisms. To assess MP's impact on the soil nitrogen cycle and related functional bacteria, we carried out a one-month soil incubation experiment using typical acidic soil. The soil was amended with alfalfa meal and was spiked with 1% and 5% (mass percentage) of low-density polyethylene (LDPE) and polyvinyl chloride (PVC) MPs. Our results showed that both LDPE and PVC addition significantly increased soil nitrification rate and nitrate reductase activity, which could further promote soil denitrification. The relative abundance of diazotrophs, ammonium oxidizing, and denitrifying bacterial groups were significantly altered with MPs addition. Moreover, the MPs treatments greatly enhanced denitrifying bacteria richness. Redundancy analysis showed that nitrate reductase activity was the most significant factor affecting the soil functional bacterial community. Correlation analysis shows that Nitrosospira genus might be for the improvement of soil nitrification rate. Our results implied that MPs exposure could significantly affect the soil nitrogen cycling in farmland ecosystems by influencing essential nitrogen functional microorganisms and related enzymatic activities.

Metabolism characteristics of nitrogen functional microorganisms in bioretention system under multiple dry-wet alternation

In this study, we used metagenomic sequencing to explore the metabolism characteristics of nitrogen-cycling microorganisms in a bioretention system under multiple dry-wet alternation conditions. The results revealed that different dry-wet alternation conditions affected the composition of nitrogen-cycling microbes in bioretention soil media (BSM). In addition, the metagenomic analyses showed a higher relative abundance of key denitrifiers (Proteobacteria and Actinobacteria) under constant periods than unordered period. Furthermore, there was increased expression of nitrification functional genes and decreased expression of denitrifying functional genes during the unordered period, leading to nitrate accumulation in BSM. Under the constant dry-wet conditions, a short dry period (5 d) was shown to be beneficial to the nitrate reduction process. However, there was a risk of nitrite accumulation, which could be improved by rewetting. Moreover, our data proved that the NADH production process was inhibited in unordered period, causing nitrate accumulating. In conclusion, this study revealed that the different dry-wet alternation conditions could drive the expression of nitrogen functional genes, thus affected the biological nitrogen metabolism in bioretention systems.

Cold-resistant performance and the promoted development of functional community with flexible metabolic patterns in a Biofilm Bio-Nutrient Removal (BBNR) system amended with supplementary carbon source for phosphorus recovery

The need for recovery of phosphorus (P) from wastewater has accelerated the retrofitting of existing bio-nutrient removal (BNR) processes into bio-nutrient removal-phosphorus recovery processes (BNR-PR). A periodical carbon source supplement is needed to facilitate the P-recovery. But the impact of this amendment on the cold resistances of the reactor and the functional microorganisms (for nitrogen and phosphorus (P) removal/recovery) are still unknown. This study presents the performances of a biofilm BNR process with a carbon source regulated the P recovery (BBNR-CPR) process operating at different temperatures. When the temperature was decreased from 25 ± 1 °C to 6 ± 1 °C, the system total nitrogen and total phosphorus removals and the corresponding kinetic coefficients decreased moderately. The indicative genes of the phosphorus-accumulating organisms (e.g., Thauera spp. and Candidatus Accumulibacter spp.) increased significantly. An increase of Nitrosomonas spp. genes aligned to polyhydroxyalkanoates (PHAs), glycine, and extracellular polymeric substance synthesis were observed, which was probably related to cold resistance. The results provide a new vision for understanding the advantages of P recovery-targeted carbon source supplementation for constructing a new type of cold-resistant BBNR-CPR processes.

The rapid start-up of CANON process through adding partial nitration sludge to ANAMMOX system

This study aimed to start up the completely autotrophic nitrogen removal over nitrite (CANON) process after adding partial nitration (PN) sludge to the ANAMMOX reactor, so as to help the rapid start-up and stable operation of the CANON process in practical engineering applications. There were three steps in the research: cultivating the PN sludge, building a reliable ANAMMMOX system, and finally starting and running the CANON process. The PN sludge was successfully cultivated in less than 45 days with around 90% nitrite accumulation rate. The ANAMMOX reactor enriched a significant quantity of red granular sludge within 70 days, achieving the maximum nitrogen removal rate of 1.74 kg/(m3·d). Eventually, the CANON reactor was started up successfully, which achieved 95.08% of average ammonium removal efficiency and 84.51% of average total nitrogen removal efficiency in 60 days. The residual recalcitrant nitrite-oxidizing bacteria in the CANON process was successfully inhibited by intermittent aeration and 12 mg/L free ammonia in UASB reactor. Besides, Candidatus Kuenenia, Candidatus Brocadia and Nitrosomonas were the main functional microorganisms involved in the CANON process.