Functional Diversity Research Articles

Land use intensification has extensive effects on the functional and phylogenetic diversity of neotropical ant communities

Land use intensification has extensive effects on the functional and phylogenetic diversity of neotropical ant communities

The E592K variant of SF3B1 creates unique RNA missplicing and associates with high-risk MDS without ring sideroblasts.

Among the most common genetic alterations in the myelodysplastic syndromes (MDS) are mutations in the spliceosome gene SF3B1. Such mutations induce specific RNA missplicing events, directly promote ring sideroblast (RS) formation, and generally associate with more favorable prognosis. However, not all SF3B1 mutations are the same, and little is known about how distinct hotspots influence disease. Here we report that the E592K variant of SF3B1 associates with high-risk disease features in MDS, including a lack of RS, increased myeloblasts, a distinct co-mutation pattern, and a lack of the favorable survival seen with other SF3B1 mutations. Moreover, compared to other hotspot SF3B1 mutations, E592K induces a unique RNA missplicing pattern, retains an interaction with the splicing factor SUGP1, and preserves normal RNA splicing of the sideroblastic anemia genes TMEM14C and ABCB7. These data have implications for our understanding of the functional diversity of spliceosome mutations, as well as the pathobiology, classification, prognosis, and management of SF3B1-mutant MDS.

Biochar addition can negatively affect plant community performance when altering soil properties in saline-alkali wetlands

Biochar is a widely proposed solution for improving degraded soil in coastal wetland ecosystems. However, the impacts of biochar addition on the soil and plant communities in the wetland remains largely unknown. In this study, we conducted a greenhouse experiment using soil seed bank from a coastal saline-alkaline wetland. Three types of biochar, including Juglans regia biochar (JBC), Spartina alterniflora biochar (SBC) and Flaveria bidentis biochar (FBC), were added to the saline-alkaline soil at ratios of 1%, 3% and 5% (w/w). Our findings revealed that biochar addition significantly increased soil pH, and increased available potassium (AK) by 3.74% - 170.91%, while reduced soil salinity (expect for 3% SBC and 5%SBC) by 28.08% - 46.93%. Among the different biochar types, the application of 5% FBC was found to be the most effective in increasing nutrients and reducing salinity. Furthermore, biochar addition generally resulted in a decrease of 7.27% - 90.94% in species abundance, 17.26% - 61.21% in community height, 12.28% - 56.42% in stem diameter, 55.34% - 90.11% in total biomass and 29.22% - 78.55% in root tissue density (RTD). In particular, such negative effects was the worst in the SBC samples. However, 3% and 5% SBC increased specific root length (SRL) by 177.89% and 265.65%, and specific root surface area (SRSA) by 477.02% and 286.57%, respectively. The findings suggested that the plant community performance was primarily affected by soil pH, salinity and nutrients levels. Furthermore, biochar addition also influenced species diversity and functional diversity, ultimately affecting ecosystem stability. Therefore, it is important to consider the negative findings indirectly indicate the ecological risks associated with biochar addition in coastal salt-alkaline soils. Furthermore, Spartina alterniflora was needed to desalt before carbonization to prevent soil salinization when using S. alterniflora biochar, as it is a halophyte.

Regional differences of functional and taxonomic bird diversity in tropical agroforests of Peru

AbstractDiversity and functionality of bird communities in tropical agroforests are shaped by their surrounding landscape, particularly the extent and type of natural forest. However, most evidence comes from tropical rainforest landscapes, whereas the bearing of such trends in other forest types remains understudied. We compared functional and beta diversity of bird communities in 23 cacao agroforests embedded in landscapes of two contrasting Peruvian regions: seasonally‐dry tropical forests and subtropical rainforests from the Andean foothills. Strong climatic seasonality affects both landscapes, but forest vegetation structure and complexity differ. We found higher bird species richness (n = 179 spp) and higher species turnover in the subtropical forest than in the dry forest landscape (n = 64 spp). Only in the dry forest landscape, distance from forest increased dissimilarity driven by species loss, that is, the nestedness component of beta diversity. This points to the importance of conserving dry tropical forests within the broader landscape matrix, with known benefits for pest control and cacao yield. Functional diversity indices were not influenced by forest distance in either landscape, but the proportion of insects in birds' diet decreased by 27% along a 1 km distance gradient from forest in the subtropical forest landscape. In the dry forest landscape, however, it decreased by only 3% across the same distance gradient. Far from forest (≥1 km), forest specialization decreased by ~33% and 20% in the subtropical and dry forest landscapes, respectively. These differences indicate that regionally‐adapted agroforest management is paramount for conserving bird diversity and ecosystem services as pest control. Procuring high‐canopy shade trees and adequate microhabitats for insectivorous species is essential to maintain biocontrol services in the subtropical forest. Conversely, in the dry forest enhancing low‐canopy vegetation with a focus on frugivores and ensuring forest closeness to agroforests may maximize bird diversity and their ecosystem services. A complete version of this article is translated to Spanish in the supplements.

Mechanisms of secondary biogenic coalbed methane formation in bituminous coal seams: a joint experimental and multi-omics study.

Coal seam microbes, as endogenous drivers of secondary biogenic gas production in coal seams, might be related to methane production in coal seams. In this study, we carried out anaerobic indoor culture experiments of microorganisms from three different depths of bituminous coal seams in Huainan mining area, and revealed the secondary biogas generation mechanism of bituminous coal seams by using the combined analysis of macro-genome and metabolism multi-omics. The results showed that the cumulative mass molar concentrations (Molality) of biomethane production increased with the increase of the coal seam depth in two consecutive cycles. At the genus level, there were significant differences in the bacterial and archaeal community structures corresponding to the three coal seams 1#, 6#, and 9#(p < 0.05). The volatile matter of air-dry basis (Vad) of coal was significantly correlated with differences in genus-level composition of bacteria and archaea, with correlations of R bacterial = 0.368 and R archaeal = 0.463, respectively. Functional gene analysis showed that the relative abundance of methanogenesis increased by 42% before and after anaerobic fermentation cultivation. Meanwhile, a total of 11 classes of carbon metabolism homologues closely related to methanogenesis were detected in the liquid metabolites of coal bed microbes after 60 days of incubation. Finally, the fatty acid, amino acid and carbohydrate synergistic methanogenic metabolic pathway was reconstructed based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. The expression level of mcrA gene within the metabolic pathway of the 1# deep coal sample was significantly higher than that of the other two groups (p < 0.05 for significance), and the efficient expression of mcrA gene at the end of the methanogenic pathway promoted the conversion of bituminous coal organic matter to methane. Therefore, coal matrix compositions may be the key factors causing diversity in microbial community and metabolic function, which might be related to the different methane content in different coal seams.

SEMANTICS OF THE CONCEPT OF “STEED”/“HORSE”IN THE RUSSIAN ART OF THE FIRST THIRD OF THE20TH CENTURY: “A HORSE AS A HORSE” AND “WE AREALL A LITTLE BIT HORSES”

The article continues the research on the artistic image in the Russian art of the first third of the 20th century and aims at studying the concepts of “steed”/“horse” in the art of the 1900s-1920s. From the beginning of revolutionary transformations in Russian art to the changes after 1917, the semantic meaning of the artistic image in its evolution is of particular interest. The basis for the study is largely fiction, in which the horse image was formed most fully. The multidimensionality of semantic relations within the boundaries of the synonymic paradigm with the general meaning of “horse” is examined in the article on the material of fine arts. The author studies the diversity of artistic roles, symbolic and semantic functions, which were given to the horse image in the art of a certain period and reveals the specificity of these roles depending on the artistic concept. Identification and recognition of the image occurs at different levels. The content of the concept is not revealed only through the plot. Unexpected semantic associations, suggested to the artist by visual, worldly, intellectual, emotional experience or creative intuition, are also possible. In this context, the quotations in the title of the article mark two main notions: “a horse as a horse” (V. Shershenevich) actualizes the direct meaning of “a horse as an animal”, and “we are all a little bit horses” (V. Mayakovsky) is a zoometaphor defining the inner state of a person. At this stage of the study, we can distinguish five main sections that allow us to structure and order the set of works containing the horse image: a village, a city, a rider, Apocalypse, a historical event (here the Civil War and collectivization). In order to fully understand the specifics of the semantic content of the horse image in the art during the study period, it is necessary to carry out some additional research. So far, the proposed text cannot claim to be an exhaustive solution to the problem, but outlines the vectors that allow us to continue its study.

Marine Pediococcus pentosaceus E3 Probiotic Properties, Whole-Genome Sequence Analysis, and Safety Assessment.

Probiotics play a significant role in enhancing health, and they are well known for bacteriocins production. Evaluating probiotics' whole-genome sequence provides insights into their consumption outcomes. Thus, genomic studies have a significant role in assessing the safety of probiotics more in-depth and offer valuable information regarding probiotics' functional diversity, metabolic pathways, and health-promoting mechanisms. Marine Pediococcus pentosaceus E3, isolated from shrimp gut, exhibited beneficial properties, indicating its potential as a probiotic candidate. Phenotypically, E3 strain was susceptible to most antibiotics assessed, tolerant to low pH and high bile salt conditions, and revealed no hemolysin activity. Interestingly, E3-neutralized CFS revealed significant antibacterial activity against pathogens under investigation. Therefore, the concentrated CFS was prepared and evaluated as a natural biopreservative and showed outstanding antimicrobial activity. Furthermore, integrated-based genome assessment has provided insight into probiotic characteristics at the genomic level. Whole-genome sequencing analysis revealed that the E3 genome possesses 1805 protein-coding genes, and the genome size was about 1.8Mb with a G + C content of 37.28%. Moreover, the genome revealed the absence of virulence factors and clinically related antibiotic genes. Moreover, several genes consistent with probiotic microorganisms' features were estimated in the genome, including stress response, carbohydrate metabolism, and vitamin biosynthesis. In addition, several genes associated with survival and colonization within the gastrointestinal tract were also detected across the E3 genome. Therefore, the findings suggest that insights into the genetic characteristics of E3 guarantee the safety of the strain and facilitate future development of E3 isolate as a health-promoting probiotic and source of biopreservative.

Functional and regulatory diversity of homeobox-leucine zipper transcription factors BnaHB6 under dehydration and salt stress in Brassica napus L.

The plant-specific homeodomain-leucine zipper I subfamily is involved in the regulation of various biological processes, particularly growth, development and stress response. In the present study, we characterized four BnaHB6 homologues from Brassica napus. All BnaHB6 proteins have transcriptional activation activity. Structural and functional data indicate the complex role of BnaHB6 genes in regulating biological processes, with some functions conserved and others diverged. Transcriptional analyzes revealed that they are induced in a similar manner in different tissues but show different expression patterns in response to stress and circadian rhythm. Only the BnaA09HB6 and BnaC08HB6 genes are expressed under dehydration and salt stress, and in darkness. The partial transcriptional overlap of BnaHB6s with the evolutionarily related genes BnaHB5 and BnaHB16 was also observed. Transgenic Arabidopsis thaliana plants expressing a single proBnaHB6::GUS partially confirmed the expression results. Bioinformatic analysis allowed the identification of TF-binding sites in the BnaHB6 promoters that may control their expression under stress and circadian rhythm. ChIP-qPCR analysis revealed that BnaA09HB6 and BnaC08HB6 bind directly to the promoters of the target genes BnaABF4 and BnaDREB2A. Comparison of their expression patterns in the WT plants and the bnac08hb6 mutant showed that BnaC08HB6 positively regulates the expression of the BnaABF4 and BnaDREB2A genes under dehydration and salt stress. We conclude that four BnaHB6 homologues have distinct functions in response to stress despite high sequence similarity, possibly indicating different binding preferences with BnaABF4 and BnaDREB2A. We hypothesize that BnaC08HB6 and BnaA09HB6 function in a complex regulatory network under stress.

Functional diversity of dopamine axons in prefrontal cortex during classical conditioning

Midbrain dopamine neurons impact neural processing in the prefrontal cortex (PFC) through mesocortical projections. However, the signals conveyed by dopamine projections to the PFC remain unclear, particularly at the single-axon level. Here, we investigated dopaminergic axonal activity in the medial PFC (mPFC) during reward and aversive processing. By optimizing microprism-mediated two-photon calcium imaging of dopamine axon terminals, we found diverse activity in dopamine axons responsive to both reward and aversive stimuli. Some axons exhibited a preference for reward, while others favored aversive stimuli, and there was a strong bias for the latter at the population level. Long-term longitudinal imaging revealed that the preference was maintained in reward- and aversive-preferring axons throughout classical conditioning in which rewarding and aversive stimuli were paired with preceding auditory cues. However, as mice learned to discriminate reward or aversive cues, a cue activity preference gradually developed only in aversive-preferring axons. We inferred the trial-by-trial cue discrimination based on machine learning using anticipatory licking or facial expressions, and found that successful discrimination was accompanied by sharper selectivity for the aversive cue in aversive-preferring axons. Our findings indicate that a group of mesocortical dopamine axons encodes aversive-related signals, which are modulated by both classical conditioning across days and trial-by-trial discrimination within a day.

Gustatory Receptor 206 Participates in the Foraging Behavior of Larvae of Polyphagous Pest Spodoptera litura.

Insect gustatory receptors (GRs) aid in the precise identification of deterrent or stimulant compounds associated with food, mating, and egg-laying. Thus, they are promising targets for developing efficient insecticides. Here, 61 GRs in the chemosensory organs of Spodoptera litura larvae and adults were identified. Among them, SlitGR206 exhibited larval labium (LL)-specific expression characteristics. To explore the role of SlitGR206, a bacterial expression system was established to produce high-quality double-stranded RNA (dsRNA) and suppress SlitGR206 expression in LL. Subsequent behavioral assessments revealed that SlitGR206 silencing influenced larval feeding preferences and absorption. Moreover, it was found to reduce the ability of larvae to forage the five crucial host odorants. These findings demonstrate that SlitGR206 likely plays an indirect regulatory role in host recognition, consequently affecting foraging behavior. This provides a crucial foundation for the analysis of functional diversity among insect GRs and the precise development of nucleic acid pesticides in the future.

Ecosystem functional types of the circumpolar Arctic tundra based on the seasonal dynamics of vegetation productivity

Abstract Biodiversity, when viewed through the combined lenses of compositional, structural, and functional attributes, provides for a holistic understanding of the complexities found within community assemblages and ecosystems. However, advancement in our understanding of how ecosystem functional diversity interacts with structural and compositional diversity metrics is lacking, in part because universally applied methodologies to derive ecosystem functional classifications are still under development and vary widely across scales, extents and biomes. This study presents a methodology to construct ecosystem functional types (EFTs), or areas of the land surface that function similarly, using the MODIS NDVI record, for the terrestrial circumpolar Arctic. EFTs were derived from the seasonal dynamics of NDVI, over the Arctic tundra at 250m resolution and compared to bioclimate subzones and to structurally and compositionally defined vegetation units of the Circumpolar Arctic Vegetation Map (CAVM). Correspondence analyses of CAVM EFTs to previously delineated CAVM bioclimatic subzones, physiognomic (vegetation) units and floristic provinces revealed a general congruence, indicating convergence across composition, structure, and function; yet also demonstrated substantial functional variability even within bioclimate subzones and vegetation units. Strong latitudinal gradients in ecosystem function are present, with EFT richness ranging from low (34) in northernmost regions to high (45) in southernmost regions. Locally, the mountainous regions of northern Alaska, and eastern and western Siberia had high spatial variability in ecosystem functioning. Aside from these generalities, we found that EFTs varied widely within individual mapped vegetation units, successfully capturing the functional dimension of biodiversity across the circumpolar Arctic tundra.

Functional diversity of dopamine axons in prefrontal cortex during classical conditioning.

Midbrain dopamine neurons impact neural processing in the prefrontal cortex (PFC) through mesocortical projections. However, the signals conveyed by dopamine projections to the PFC remain unclear, particularly at the single-axon level. Here, we investigated dopaminergic axonal activity in the medial PFC (mPFC) during reward and aversive processing. By optimizing microprism-mediated two-photon calcium imaging of dopamine axon terminals, we found diverse activity in dopamine axons responsive to both reward and aversive stimuli. Some axons exhibited a preference for reward, while others favored aversive stimuli, and there was a strong bias for the latter at the population level. Long-term longitudinal imaging revealed that the preference was maintained in reward- and aversive-preferring axons throughout classical conditioning in which rewarding and aversive stimuli were paired with preceding auditory cues. However, as mice learned to discriminate reward or aversive cues, a cue activity preference gradually developed only in aversive-preferring axons. We inferred the trial-by-trial cue discrimination based on machine learning using anticipatory licking or facial expressions, and found that successful discrimination was accompanied by sharper selectivity for the aversive cue in aversive-preferring axons. Our findings indicate that a group of mesocortical dopamine axons encodes aversive-related signals, which are modulated by both classical conditioning across days and trial-by-trial discrimination within a day.

Genome-wide identification and expression analysis of the Eriobotrya japonica TIFY gene family reveals its functional diversity under abiotic stress conditions

BackgroundPlant-specific TIFY proteins are widely found in terrestrial plants and play important roles in plant adversity responses. Although the genome of loquat at the chromosome level has been published, studies on the TIFY family in loquat are lacking. Therefore, the EjTIFY gene family was bioinformatically analyzed by constructing a phylogenetic tree, chromosomal localization, gene structure, and adversity expression profiling in this study.ResultsTwenty-six EjTIFY genes were identified and categorized into four subfamilies (ZML, JAZ, PPD, and TIFY) based on their structural domains. Twenty-four EjTIFY genes were irregularly distributed on 11 of the 17 chromosomes, and the remaining two genes were distributed in fragments. We identified 15 covariate TIFY gene pairs in the loquat genome, 13 of which were involved in large-scale interchromosomal segmental duplication events, and two of which were involved in tandem duplication events. Many abiotic stress cis-elements were widely present in the promoter region. Analysis of the Ka/Ks ratio showed that the paralogous homologs of the EjTIFY family were mainly subjected to purifying selection. Analysis of the RNA-seq data revealed that a total of five differentially expressed genes (DEGs) were expressed in the shoots under gibberellin treatment, whereas only one gene was significantly differentially expressed in the leaves; under both low-temperature and high-temperature stresses, there were significantly differentially expressed genes, and the EjJAZ15 gene was significantly upregulated under both low- and high-temperature stress. RNA-seq and qRT-PCR expression analysis under salt stress conditions revealed that EjJAZ2, EjJAZ4, and EjJAZ9 responded to salt stress in loquat plants, which promoted resistance to salt stress through the JA pathway. The response model of the TIFY genes in the jasmonic acid pathway under salt stress in loquat was systematically summarized.ConclusionsThese results provide a theoretical basis for exploring the characteristics and functions of additional EjTIFY genes in the future. This study also provides a theoretical basis for further research on breeding for salt stress resistance in loquat. RT-qPCR analysis revealed that the expression of one of the three EjTIFY genes increased and the expression of two decreased under salt stress conditions, suggesting that EjTIFY exhibited different expression patterns under salt stress conditions.

From the archives: Targeting proteins to plasmodesmata, germination in the desert, and functional diversity of KNOX proteins.

From the archives: Targeting proteins to plasmodesmata, germination in the desert, and functional diversity of KNOX proteins.

Iron-sulfur protein odyssey: exploring their cluster functional versatility and challenging identification.

Iron-sulfur (Fe-S) clusters are an essential and ubiquitous class of protein-bound prosthetic centers that are involved in a broad range of biological processes (e.g. respiration, photosynthesis, DNA replication and repair and gene regulation) performing a wide range of functions including electron transfer, enzyme catalysis, and sensing. In a general manner, Fe-S clusters can gain or lose electrons through redox reactions, and are highly sensitive to oxidation, notably by small molecules such as oxygen and nitric oxide. The [2Fe-2S] and [4Fe-4S] clusters, the most common Fe-S cofactors, are typically coordinated by four amino acid side chains from the protein, usually cysteine thiolates, but other residues (e.g. histidine, aspartic acid) can be found. While diversity in cluster coordination ensures the functional variety of the Fe-S clusters, the lack of conserved motifs makes new Fe-S protein identification challenging especially when the Fe-S cluster is also shared between two proteins as observed in several dimeric transcriptional regulators and in the mitoribosome. Thanks to the recent development of in cellulo, in vitro and in silico approaches, new Fe-S proteins are still regularly identified, highlighting the functional diversity of this class of proteins. In this review, we will present three main functions of the Fe-S clusters and explain the difficulties encountered to identify Fe-S proteins and methods that have been employed to overcome these issues.

Zooplankton functional diversity in subtropical reservoirs of distinct trophic states (São Paulo state, Brazil).

Studies on functional traits of aquatic communities are useful for understanding the ecosystem dynamics as well as the diversity of ecological niches. Here, we characterize zooplankton functional groups and which limnological factors are responsible to changes in traits. Water samples were collected to evaluate limnological parameters and vertical hauls with plankton net (68μm) were performed to characterize the community in seven reservoirs (Itupararanga, Atibainha, Salto Grande, Rio Grande, Igaratá, Barra Bonita, and Broa, São Paulo state, Brazil). Each species identified was classified according to a trophic group, reproduction mode, body length, habitat, and feeding habitats. Our results showed a predominance of pelagic suspensory herbivores with cilia (31%) followed by pelagic herbivore suspension filter feeders (17%) and raptorial omnivores (15.38%). The other individuals were categorized as pelagic herbivore suspension with oral device (12.3%), littoral herbivores suspensive with cilia (12.3%), pelagic-sucking herbivores (9.2%), and littoral grazing herbivores (3%). The dominance of herbivores may be influenced by the availability of nutrients, influencing their food sources. The abundance of omnivores engaged in predatory behavior can be attributed to disponible prey, thereby exerting significant repercussions on the organization of biological communities.

Reinforcements in the face of ongoing threats: a case study from a critically small carnivore population

AbstractReinforcements are a well‐established tool for alleviating small population pressures of inbreeding and genetic diversity loss. Some small populations also suffer from specific threats that pose a discrete selective pressure, like diseases. Uncertainty about reinforcing diseased populations exists, as doing so may increase disease prevalence and disrupt potential adaptive processes. However, without assisted gene flow, isolated populations are at high risk of extinction. Tasmanian devils (Sarcophilus harrisii) are a useful case study to test whether reinforcements can alleviate small‐population pressures where there is an ongoing disease pressure. We investigated demographic, genome‐wide and functional genetic diversity, and disease consequences of reinforcing a small population (&lt;20 animals) that was severely impacted by devil facial tumour disease. Released animals from one source population successfully bred with incumbent individuals, tripling the population size, improving genome‐wide and functional diversity and introducing 26 new putatively functional alleles, with no common alleles lost and no increase in disease prevalence. Results suggest, in the case of Tasmanian devils, reinforcements can alleviate small‐population pressures without increasing disease prevalence. Because no common functional alleles were lost, it is likely that any adaptive processes in response to the disease may still occur in the reinforced population, perhaps even with greater efficiency due to reduced genetic drift (due to larger population size). Our study is presented as a comprehensive worked example of the IUCN's guidelines for monitoring reinforcements, to showcase the value of genetic monitoring in a richly monitored system and provide realistic approaches to test similar questions in other taxa.

Molecular-level carbon traits underlie the multidimensional fine root economics space.

Carbon influences the evolution and functioning of plants and their roots. Previous work examining a small number of commonly measured root traits has revealed a global multidimensionality of the resource economics traits in fine roots considering carbon as primary currency but without considering the diversity of carbon-related traits. To address this knowledge gap, we use data from 66 tree species from a tropical forest to illustrate that root economics space co-varies with a novel molecular-level traits space based on nuclear magnetic resonance. Thinner fine roots exhibit higher proportions of carbohydrates and lower diversity of molecular carbon than thicker roots. Mass-denser fine roots have more lignin and aromatic carbon compounds but less bioactive carbon compounds than lighter roots. Thus, the transition from thin to thick fine roots implies a shift in the root carbon economy from 'do-it-yourself' soil exploration to collaboration with mycorrhizal fungi, while the shift from light to dense fine roots emphasizes a shift from acquisitive to conservative root strategy. We reveal a previously undocumented role of molecular-level carbon traits that potentially undergird the multidimensional root economics space. This finding offers new molecular insight into the diversity of root form and function, which is fundamental to our understanding of plant evolution, species coexistence and adaptations to heterogeneous environments.

The bi-directional relationships between diversified leisure activity participation and cognitive function in older adults in China: separating between-person effects from within-person effects

ObjectiveTo examine the bi-directorial association between diversified leisure activity participation and cognitive function over a 7-year period.MethodsData analyzed was from the Chinese Longitudinal Healthy Longevity Survey (CLHLS), a large-scale longitudinal national study. The baseline survey was conducted in 2011 with follow-up every three years. We traced a total of 2718 participants over a period of 7 years. We used adjusted random intercept cross-lagged panel models (RI-CLPMs) to examine the bi-directorial associations between diversified leisure activity participation and cognitive function.ResultsWe observed bi-directorial associations between diversity of leisure activity and cognitive function across waves at the between-person and within-person levels. The adjusted random intercept cross-lagged panel models fitted the data appropriately, and the 3-year cross-lagged effects of prior diversified leisure activity participation on cognitive function (β = 0.058, p < 0.01) and cognitive function on subsequent diversified leisure activity participation (β = 0.047, p < 0.05) were significant. The results remained after adjusting the model for baseline sex, age, educational level, marital status and current residence, the number of chronic diseases, ADL, depressive symptoms, sleep quality, smoking, and drinking.ConclusionThis study suggests that a reciprocal causality relationship between diversified leisure activity participation and cognitive function, indicating a “positive circle” that further promotes cognition over time.

Nutrient contents and microbial communities as mediators of the effects of land-use in ecosystem functioning in alpine ecosystems from Central Mexico

Abstract Purpose The interplay between microbial taxonomy, functional diversity, and land-use changes across diverse ecosystems must be better understood. This study explores, through a statistical modeling approach, the intricate relationships between microbial communities, soil properties (nutrient contents and physicochemical parameters), and land-use changes, using stable isotopes ratio (δ13C/δ15N) as ecosystem function indicator. Methods Using Structural Equation Modeling (SEM) this study aims to investigate how soil microbial communities, soil properties and ecosystem function are interconnected in three ecosystems undergoing land-use change in Nevado de Toluca, Mexico. Results Multivariate analyses of microbial communities and soil parameters for the different land-uses reveal significant variation in both microbial composition and soil properties (i.e. nutrient contents) mainly explained by the land-use history of the studied ecosystems. Through the SEM approach, it was possible to disentangle the relative contribution of land-use change, microbial community composition and nutrient contents to the ecosystem function defined as the δ13C/δ15N ratio. Conclusions Microbial communities are the main drivers of soil ecosystem functioning. However, through SEM approach it is possible to formally test direct and indirect relationships impacting microbial communities and the resulting functional consequences, contributing to a mechanistic understanding of the ecological implications of land-use change, aiding in sustainable land-use decision-making.