Functional Beverages Research Articles

Effects of Emulsifiers on Physicochemical Properties and Carotenoids Bioaccessibility of Sea Buckthorn Juice.

The need to improve the physicochemical properties of sea buckthorn juice and the bioavailability of carotenoids is a major challenge for the field. The effects of different natural emulsifiers, such as medium-chain triglycerides (MCTs), tea saponins (TSs) and rhamnolipids (Rha), on the physical and chemical indexes of sea buckthorn juice were studied. The particle size of sea buckthorn juice and the carotenoids content were used as indicators for evaluation. The effects of different addition levels of MCT, Rha and TS on the bioavailability of carotenoids in sea buckthorn juice were investigated by simulating human in vitro digestion tests. The results showed that those emulsifiers, MCT, Rha and TS, can significantly reduce the particle size and particle size distribution of sea buckthorn juice, improve the color, increase the soluble solids content, turbidity and physical stability and protect the carotenoids from degradation. When the addition amount of Rha was 1.5%, the total carotenoids content (TCC) of sea buckthorn juice increased by 45.20%; when the addition amount of TS was 1.5%, the total carotenoids content (TCC) of sea buckthorn juice increased by 37.95%. Furthermore, the bioaccessibility of carotenoids was increased from 36.90 ± 2.57% to 54.23 ± 4.17% and 61.51 ± 4.65% through in vitro digestion by Rha and TS addition, respectively. However, the total carotenoids content (TCC) of sea buckthorn juice and bioaccessibility were not significantly different with the addition of MCT. In conclusion, the findings of this study demonstrate the potential of natural emulsifiers, such as MCT, Rha and TS, to significantly enhance the physicochemical properties and bioavailability of carotenoids in sea buckthorn juice, offering promising opportunities for the development of functional beverages with improved nutritional benefits.

Initial Development of Lamota (Salicornia europae) Yogurt As a Functional Drink

Salicornia europae is a marine plant that grows in coastal areas and tides. In Sumbawa, this plant lives in the former area of ponds and usually called as lamota. S. europae contains beneficial ingredients such as iodine, minerals, vitamins, antioxidants, unsaturated fatty acids, and sugars. Marine plant innovations in S. Europae, which is rich in iodine into processed yogurt, has not been well explored. This study aimed to develop the processing of lamota into functional yogurt drink. Complete Randomized Design (CRD) was used in this experiment with a single factor of 6 levels of lamota concentration. Iodine content, ash content, pH and scoring test were carried out to find out the best quality of the lamota yogurt. The results showed that the iodine content of the product was 29.01 mcg / g dry weight and ash content was 10.59%. The pH showed that the addition of lamota minimally affected the pH of the yoghurt. In the organoleptic test found that 25% addition of lamota extract was the most preferrable concentration compared to 5, 10, 15, and 20% based on the color, taste, texture, aroma. Panelist describe the 25% lamota addition has greenish color, a little bit salty, watery texture, and green aroma. Based on the iodine content and the best consumers acceptance the yogurt with 25% addition of lamota could be categorized as a rich in iodine drink and a promising product to improve the functional characteristics of yogurt as well develop a value added local marine commodity with unique flavor.

Potensi Antibakteri Minuman Fungsional Tradisional Jawa (Wedang Uwuh) Berdasarkan Variasi Waktu Rebusan

Wedang uwuh is a functional beverage prepared by boiling a combination of spices, namely sappan wood, ginger, nutmeg leaves, cinnamon leaves, cloves, and clove leaves. It is reported to have antioxidant, antibacterial, and immune-enhancing properties due to its active compounds. This traditional beverage shows potential in reducing infections caused by Escherichia coli, highlighting the importance of proper preparation methods. The objective of this study was to evaluate the antibacterial activity of wedang uwuh prepared by different boiling duration (5, 10, 15, and 20 min) to determine the optimal time for maximum effectiveness. Phytochemical changes, as indicated by color alterations, were assessed by a descriptive approach while a disc diffusion method was used as an antibacterial assay to measure the inhibition zone diameter against E. coli. Compounds in each treatment were analyzed using GC-MS. Phytochemical screening confirmed the presence of alkaloids, flavonoids, saponins, triterpenoids, and tannins in all variations. Antibacterial testing revealed that boiling for 15 min exhibited the highest activity, with an inhibition zone diameter of 10.43±1.33 mm, reflecting strong antibacterial efficacy. This result significantly differed from the 5 and 10 min treatments. The 5 min boiling treatment demonstrated a smaller inhibition zone with an average diameter of 5.71±1.67 mm, indicating moderate antibacterial activity, but it did not differ significantly from the 10 min treatment. A chemical analysis by GC-MS for the 15 min boiling sample successfully identified 23 compounds, and among these chemicals, hexadecanoic acid was found as the predominant component.

Enhanced production of gamma‐aminobutyric acid in fermented carrot juice by utilizing pectin hydrolysate derived from pomegranate waste

AbstractIn this study, a functional fermented beverage enriched with gamma‐aminobutyric acid (GABA) was produced. To achieve this, the prebiotic abilities of pectin obtained from pomegranate peel and its enzymatic hydrolysates were evaluated. Additionally, a functional fermented beverage enriched with GABA was produced by fermenting carrot juice with pectin hydrolysates. First, pectin was obtained at a yield of 8.91% from pomegranate peels. Pectinase‐catalyzed hydrolysis of the obtained pectin was applied using different enzyme concentrations and hydrolysis times, and the effect of these hydrolysates on the growth of Levilactobacillus brevis was determined. Although the Fourier transform infrared (FT‐IR) spectra of the resulting hydrolysates were similar, their degree of esterification compared to that of pectin was statistically different (p < .05). Considering the viability analysis and GABA production of L. brevis in the liquid medium supplemented with pectin or its hydrolysate, the hydrolysate obtained by treatment with 400 μL enzyme for 2 h and having a high glucose content (216.80 mg/100 g) was selected for application in fermented carrot juice. During fermentation (24, 48, and 72 h), a remarkable change was observed, especially in the amounts of lactic acid and malic acid, while the amount of GABA in carrot juice varied between 25 and 46 mg/mL and increased with the increase in hydrolysate concentration. It was observed that the total phenolic content and antioxidant activity of carrot juice were highly affected by the hydrolysate concentration. This study demonstrated that pectin hydrolysate obtained from food waste could be a potential prebiotic and could be used in the production of a functional beverage with improved GABA content.

Consumption status of functional drinks based on the theory of planned behavior and the stages of change model in female employees

This study aimed to determine the consumption status of functional drinks based on the theory of planned behavior and the Stages of Change Model. This study was conducted on 536 female employees of Birjand offices in 2022. The data was collected by using the functional food questionnaire and was analyzed using SPSS with significance level of P < 0.05. The largest number of people consume probiotic drinks (buttermilk, kefir, etc.) were in the maintenance stages (31.2%) and who consume milk fortified with vitamin D were in the contemplation stage (37.3%). The mean score of the participants in the subscales of attitude, behavioral control, and subjective norm was 17.69 ± 3.05, 16.83 ± 2.88, 21.73 ± 4.33, respectively. The mean score of the attitude and subjective norm regarding the consumption of all drinks had a significant relation with the stages of change model (p < 0.05). The results of this study showed that the most drinks that female employees consumed regularly were probiotic drinks, while they did not intend to use functional juices (pre-contemplation stage). Therefore, it seems that this theory can be used as a framework in designing educational programs in order to increase the consumption of functional foods and improve women's health.

GABA-fortified synbiotic guava beverage: formulation and evaluation of neuroprotective properties using cell-based assays

In recent years, the use of fermented fruit juices to offer protection against mental illness has been increasing enormously. The present study, aimed at formulating a synbiotic fruit beverage for mental well-being using fructo-oligosaccharide (FOS), Gamma-aminobutyric acid (GABA) and Lactobacillus rhamnosus. Guava extract (abbreviated as fruit extract, FE; 10%) supplemented with FOS (1%) fermented for 48 h using L. rhamnosus, was then fortified with 0.05% GABA (FE + FOS + GABA). This GABA-fortified fermented guava beverage exhibited significant in-vitro antioxidant activity as measured by using 2,2-diphenyl-1-picrylhydrazyl (IC50 value of 0.451 ± 0.05 mg/ml) and ferrous reducing antioxidant capacity (A700 nm value of 0.68 ± 0.06) assays. Fermentation also enhanced total phenolics and flavonoids by 27.63% and 27.41%, respectively. Furthermore, GABA-fortified fermented guava beverage showed a significant neuroprotective effect by negatively modulating oxidative stress and upregulating activity of antioxidant enzymes (superoxide dismutase and catalase) in rotenone treated neuronal cell lines. Taken together, our research findings clearly indicate the propensity of GABA-fortified fermented guava beverage to protect against rotenone mediated neuronal cell damage. Even though the underlying molecular mechanisms need further research, in conclusion, our study demonstrates that the GABA-fortified fermented guava beverage effectively confers neuroprotective effects in vitro, suggesting its potential as a functional beverage for promoting brain health.Graphical

Bioconversion of mangosteen pericarp extract juice with bacterial cellulose production and probiotic integration: A promising approach for functional food supplement development

This study optimized bioconversion process of mangosteen pericarp extract juice (MPEj) with a single-culture of Komagataeibacter xylinus TISTR 1061, a bacterial cellulose-producing strain and a co-culture with the probiotic Lactiplantibacillus plantarum. K. xylinus TISTR 1061 was identified as the optimal bacterial cellulose-producing strain for fermenting MPEj. Optimal conditions were determined to be 20% (v/v) MPEj supplemented with 7% (w/v) sucrose and 0.2% (w/v) ammonium dihydrogen phosphate as carbon and nitrogen sources, respectively. The growth profile of single K. xylinus was not significantly different from those of the co-culture. They showed good adaptability and cohabited during fermentation without antagonistic effects. Dynamic changes in total titratable acidity (TTA), total soluble solids (TSS), and pH during fermentation were relatively similar for both single- and co-cultures. Post-fermented MPEj with co-culture exhibited significantly higher bioactive activities, particularly antioxidant properties. Significant variations in metabolite concentrations, including 2-aminobutyrate, valine, xylitol, propionate, isoleucine, glucose, and glycerol, were observed. These compounds exhibited significantly higher average concentrations after fermentation, highlighting their potential as biomarkers distinguishing pre- and post-fermentation MPEj. Bacterial cellulose (BC) from single culture exhibited greater thickness and weight, with a more complex and branched structure, while that from co-culture was infused with probiotic cells, showing less complexity allowing the BC from co-culture demonstrated low hardness and chewiness values. The properties of both bioconverted MPEj and BC obtained as observed offer potential for innovative functional drinks and/or dietary supplements with enhanced health benefits.

Formulation and evaluation of physical, chemical and sensory properties of instant functional beverage powder containing Pathum Thani fragrance rice, soy protein and milk powder

The purpose of this study was to determine the influence of proportion and ratio of the following main ingredients comprising Pathum Thani fragrance rice powder, soy protein and milk powder on physicochemical properties and sensory characteristics of instant functional beverage powder. The experimental procedures consisted of prerequisite processing steps of cooking, drying, crushing and mixing all ingredients. The design was used to produce a total of ten samples of powdered beverage products. Whereas, dry samples and complete dissolution samples were analyzed for their physicochemical properties and sensory characteristics. Results indicated that the prepared beverage powder with various ratios of ingredient combinations delivered significant impact on their physical chemical and sensory characteristics. Principal component analysis (PCA) was implemented to group the attributes into three key dimensions that explained 76.51% of the total variance. Furthermore, Partial least squares regression (PLSR) was employed as a multivariate statistical tool to accurately determine carbohydrate, fiber, L* (dissolve), a* (dissolve) and b* (dissolve) as key drivers of liking. Having the highest consumer satisfaction levels and highest average liking scores of all sensory characteristics, the optimum formulation for instant functional beverage powder was formula 10 containing 23.34% of Pathum Thani fragrance rice powder, 13.33% of soy protein and 13.33% of milk powder, 20% of sugar, 10% of cocoa powder, 10% of malt powder, 5% of non-dairy cream powder and 5% inulin powder. More importantly, these research findings may influence decisions at many levels in increasing consumer acceptance of the product, manufacturing healthier products for people, and adding value to products of agriculture.

Transcriptome and metabolome reveal the effects of ABA promotion and inhibition on flavonoid and amino acid metabolism in tea plant.

Flavonoids (especially anthocyanins and catechins) and amino acids represent a high abundance of health-promoting metabolites. Although we observed abscisic acid accumulation in purple leaves and low levels in albino tea leaves, the specific mechanism behind its impact on flavor compounds remains unclear. In this study, we treated tea leaves with exogenous abscisic acid and abscisic acid biosynthesis inhibitors (Flu), measured physiological indicators and conducted comprehensive transcriptomic and metabolomic analyses to elucidate the potential mechanisms underlying color change. Our results demonstrate that abscisic acid treatment induces purple coloration, while Flu treatment causes discoloration in tea leaves. Metabolomic analysis revealed higher levels of four anthocyanins and six catechins in the group treated with abscisic acid in comparison with the control group. Additionally, there was a notable increase in 15 amino acids in the Flu-treated group. Notably, the levels of flavonoids and amino acids showed an inverse relationship between the two treatments. Transcriptomic comparison between the treatments and the control group revealed upregulation of differentially expressed genes encoding dihydroflavonol reductase and uridine diphosphate-glycose flavonoid glycosyltransferase in the abscisic acid-treated group, leading to the accumulation of identified anthocyanins and catechins. In contrast, differentially expressed genes encoding nitrate reductase and nitrate transporter exhibited elevated expression in the group treated with Flu, consequently facilitating the accumulation of amino acids, specifically L-theanine and L-glutamine. Furthermore, our co-expression network analysis suggests that MYB and bHLH transcription factors may play crucial roles in regulating the expression of differentially expressed genes involved in the biosynthesis of flavonoids and amino acids. This study provides insights for targeted genetic engineering to enhance the nutritional and market value of tea, together with the potential application of purple and albino tea leaves as functional beverages. It also offers guidance for future breeding programs and production.

PELATIHAN PEMBUATAN TEH HERBAL KULIT BUAH PALA DI DESA LILIBOOI, KECAMATAN LEIHITU BARAT, KABUPATEN MALUKU TENGAH

Tea is a drink that has long been known and has experienced a lot of food diversification in producing various types of tea drinks whose raw materials do not come from Camelia sinensis tea leaves but come from flowers, seeds, leaves, roots, fruit peel, and fruit flesh from various This type of plant is also known as herbal tea. The herb has a distinctive aroma, taste, and bioactive components that benefit health. Herbal tea of nutmeg peel has advantages over non-herbal tea, namely that it is effective as a functional drink or can also be used as a refreshing drink for the body. In making tea, a drying process is carried out, which aims to reduce the water content to a specific limit in the raw materials and inhibit microbial growth so that herbal tea can be stored for a long time and is not easily damaged during storage. Several drying methods can be used to make herbal tea, one of which is roasting. The roasting process in making this tea will result in the product experiencing color, texture, and flavor changes. Through this activity, the community's knowledge, skills, and herbal tea products produced can be used as business opportunities

A mini review: Potential synergy of cacao leaves and cinnamon bark as a functional tea

Abstract Cacao leaves tea is an increasingly popular drink, especially among tea lovers. Cacao leaves provide a distinctive taste and aroma. Traditionally cacao tea has been used as a herbal drink to help treat fevers, stomach aches and even coughs. The bioactive compound in cacao leaves has anti-inflammatory and antioxidant properties that can help in the healing process. Cinnamon is a spice that is widely used in food to enhance taste and aroma. It has a high antioxidant content and has many health benefits, including being an anti-diabetic. The aim of writing this article is to explain the potential synergy of cacao leaves with the addition of cinnamon as a functional drink in terms of nutritional composition and bioactive compounds contained in both. In the current era of globalization, increasing levels of free radicals have driven an increase in the need for bioactive compounds with antioxidant properties. Consuming antioxidants obtained from cacao leaves – cinnamon tea, is expected to help reduce oxidative stress and potentially provide beneficial health effects. It was found that cacao leaves tea with the addition of cinnamon bark has potential as a functional drink because it contains bioactive compounds, especially phenolics, flavonoids and alkaloids in significant concentrations.

РОЗРОБЛЕННЯ БІОТЕХНОЛОГІЇ КИСЛОМОЛОЧНИХ НАПОЇВ З БІОАКТИВНИМИ ФІТОЕКТРАКТАМИ СОЛОДКИ

A functional fermented milk drink with plant extracts for prevention and recovery was studied. The data of classical fermentation were used, in particular, the peculiarities of the preparation of phytoextracts for the development of a functional fermented milk drink. The recipe was developed and the influence of licorice root extract concentration and the type of bacterial starter on the physical, chemical and organoleptic characteristics and biological activity of the biobeverage was studied. The specifics of fermentation were taken into account when developing a technological scheme for the pilot production of a functional fermented milk drink with licorice root phytoextract.

Antioxidant Status, Physicochemical and Immune-boosting Characteristics of New Formulated Functional Beverages

Antioxidant Status, Physicochemical and Immune-boosting Characteristics of New Formulated Functional Beverages

Formula Optimization of Traditional Functional Beverage Minasarua from Bima West Nusa Tenggara Indonesia

Formula Optimization of Traditional Functional Beverage Minasarua from Bima West Nusa Tenggara Indonesia

Potential for Strengthening Immune Function and Sports Performance with Lactobacillus pentosus b240

PURPOSE: Recently, functional beverages have played a crucial role in supplementing basic nutrition and providing performance-enhancing and health benefits. Among these beverages, those containing Lactobacillus pentosus strain b240 (b240) have been utilized to promote the health and performance of athletes. This study aims to provide an overview of recent research findings regarding the nutritional and physiological efficacy of a novel functional beverage containing probiotic b240 for enhancing sports performance and promoting health.METHODS: This review collected and analyzed data from previous studies to evaluate the nutritional and physiological benefits of a novel functional beverage containing probiotic b240 for enhancing exercise performance and health. The studies were obtained through online databases and search engines, including PubMed, Web of Science, and Google Scholar.RESULTS: Beverages containing b240 positively affect immune strengthening, offering significant physiological and nutritional advantages. These innovative conditioning beverages enhance sports performance through their biological benefits and promise to improve secretory immunoglobulin A (sIgA), promoting overall health. Additionally, the potential applications of b240 in enhancing the immune system and performance could be significant for public health, particularly given the common obstacle of time constraints to regular exercise.CONCLUSIONS: These results indicate the need for deep insights into effective nutritional and exercise management strategies for enhancing immune function and sports performance. Further studies are required to investigate the effects of probiotic b240 conditioning beverages on exercise performance and healthcare.

Daily white tea intake reprograms hepatic metabolism and improves the enzymatic antioxidant defence system of rats with prediabetes

BackgroundPrediabetes (PrDM) poses a significant risk for the development of Diabetes Mellitus (DM). Preventing the transition to type 2 DM is of utmost importance, and identifying novel dietary agents with both antioxidant and antidiabetic properties is crucial. This study aims to assess the potential of regular white tea (WTEA) consumption in alleviating the detrimental effects of PrDM on hepatic metabolism and redox state. MethodsTo achieve this, we divided 18 male Wistar rats into three groups: a control group and two prediabetes (PrDM) groups. In one of the PrDM groups, water consumption was replaced with white tea (WTEA). After a two-month period, we evaluated the hepatic metabolic profile using 1H NMR. Additionally, we measured total antioxidant potential, the activities of antioxidant enzymes, and various oxidative parameters, including protein nitration and carbonylation, lipid peroxidation, and levels of H2A.X and phosphorylated H2A.X. ResultsPrDM induced a decline in hepatic catabolic metabolism and the activities of key antioxidant enzymes, including superoxide dismutase, glutathione reductase, catalase, and levels of phosphorylated H2A.X. However, when PrDM rats consumed WTEA, a remarkable restoration occurred. Specifically, WTEA intake reinstated glutathione reductase and catalase activities and elevated superoxide dismutase activity in the liver compared to normoglycemic conditions. Moreover, enhancements were noted in overall antioxidant capacity and a reduction in lipid peroxidation in the rat liver. ConclusionPrDM significantly impacts liver metabolism and antioxidant defenses, but the consumption of WTEA, a plant-based functional beverage, exhibits potent hepatoprotective capabilities against PrDM-induced oxidative stress.

Studying the technology of preparing a functional drink based on pumpkin and quince

Studying the technology of preparing a functional drink based on pumpkin and quince

Utilization of Functional Drinks Based on Mango (Mangifera indica L) Leaves and Sappanwood (Caesalpinia sappan L) as Antidiabetic

Diabetes melitus (DM) merupakan penyakit gangguan metabolik menahun akibat pankreas tidak meproduksi cukup insulin atau tubuh tidak dapat menggunakan insulin yang diproduksi secara efektif. Daun Mangga Madu (Mangifera indica L.) dan kayu secang ( Caesalpinia sappan L) adalah tanaman herbal lokal yang pemanfaatannya belum opimal. Salah satu usaha yang dilakukan adalah mengkombinasian serbuknya menjadi minuman fungsional antidiabetes. Kombinasi serbuk daun mangga madu dan kayu secang diharapkan mampu meningkatkan fungsi optimalnya sebagai minuman fungsional bagi penderita diabetes. Penelitian ini bertujuan untuk mengetahui nilai aktivitas antidiabetes,antioksidan dan kadar total fenol. Penelitian ini menggunakan metode eksperimental. Penelitian ini menggunakan metode rancangan acak lengkap (RAL) dengan 1 faktor dan lima perlakuan. Hasil analisa fitokimia mengindikasikan bahwa ekstrak etanol Daun Mangga Madu dan kayu secang mengandung senyawa metabolit sekunder Alkaloid, Tanin, Steroid terpenoid dan Flavanoid. Hasil penelitian meununjukkan bahwa kelima formulasi minuman fungsional mengandung Aktivitas antidiabetes tertinggi sebesar 92,71%, Aktivitas daya hambat antioksidan tertinggi sebesar 90,241 %. Dan kadar fenol total tetinggi sebesar 1.042%.

Metabolite profiling of butterfly pea flower (Clitoria ternatea L.) in the flowering development phase

The butter pea flower (Clitoria ternatea L.) has long been known as a medicinal plant because it contains various metabolite compounds, the content of metabolite compounds that are influenced by plant development. So it is necessary to conduct metabolomics profiling research to determine the metabolite compounds found in butterfly pea flowers in the flower bud, blooming, and wilting phases. Metabolite profiling from ethanol extract in the development phase of butterfly pea flowers in the bud, blooming, and wilting phases using GC-MS analysis identified 20 metabolite compounds each with butterfly pea flowers in the bud phase having the dominant compound Dihydroxyacetone, blooming flowers found the compound 3-O-Methyl-d-glucose, and n-Hexadecanoic acid compound withered flowers. Three dominant compounds were found in all development phases, namely 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl; Dihydroxyacetone; Benzoic acid, ethyl ester; and Dehydroxyacetone. The unique metabolite compound 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl is included in the flavonoid group, especially anthocyanin which has the potential to be an antioxidant, antibiotic, antidiabetic, anti-inflammatory, and immunomodulatory so it can be used as a natural coloring agent for functional foods or drinks and herbal medicines.

ANALISIS KADAR GULA TOTAL, FLAVONOID, DAN UJI AKTIVITAS ANTIOKSIDAN DARI FORMULASI BROTOWALI DENGAN APEL MANALAGI SEBAGAI MINUMAN FUNGSIONAL

Currently, the highest number of deaths in Indonesia is caused by non-communicable diseases. One of the non-communicable diseases is diabetes mellitus which is a metabolic disease. Efforts to prevent diabetes mellitus with non-pharmacological therapy can use plants that have the potential to have anti-diabetic effects. This study aims to make brotowali with manalagi apples as a functional drink which has potential as an antidiabetic and to determine total sugar content, flavonoids, and antioxidant activity with 3 ratios of brotowali : manalagi apple (F1 1:2, F2 1:1, and F3 2:1). This study used a completely randomized design (CRD) with 3 formulations and 3 repetitions. The statistical tests used were Analysis of Variance (ANOVA) and Duncan's Multiple Range Test (DMRT). The results showed that F1, F2, and F3 averaged total sugar content respectively 27.4%, 22.3% and 20.9%, the average of flavonoids was 41.7 mgQE/g, 48.8 mgQE/g, and 62.8 mgQE/g, and the average antioxidant activity with IC50 values were 145.5 µg/ml, 109.3 µg/ml, and 92.2 µg/ml. The best formulation of brotowali functional drink with manalagi apple is formula F3 with total sugar content of 20.9%, flavonoid content of 62.8 mgQE/g and antioxidant activity with IC50 value of 92.2 µg/ml.