Functional Activity Of Neutrophilic Granulocytes Research Articles

Neutrophil granulocyte functional status and expression of apoptosis markers in children with type 1 diabetes

In recent years, polymorphonuclear leukocytes have been proven to play an important role in the development of diabetes mellitus (DM). Neutrophil dysfunction contributes to pancreatic tissue damage as well as an increased susceptibility to infection in type 1 DM (T1DM) patients. Objective. The objective of this study was to investigate the functional activity of neutrophil granulocytes (NGs) in children with T1DM. Materials and Methods. This study involved 25 children aged 7?15 years. To evaluate programmed cell death, the number of NGs expressing apoptosis markers (CD95, CD95L and BCL2) was determined. The functional activity of NGs was determined in terms of phagocytosis and the levels of myeloperoxidase, lysosomal cationic proteins and active oxygen radicals. Results. A reduction in the bactericidal activity of NGs with deficiencies in phagocytosis, secretion of active oxygen radicals and functional reserve was found. An increase in the apoptotic potential of NGs was demonstrated, which was accompanied by an increase in CD95 expression and a decrease in BCL2 expression. An increase in the cytotoxic potential of neutrophils in the form of enhanced levels of myeloperoxidase and lysosomal cationic proteins was revealed. Conclusions. Therefore, an increase in the apoptotic potential of NGs associated with functional and metabolic changes may reflect the active involvement of NGs in the immunopathogenesis of T1DM.

Динамика показателей иммунного статуса студенток медицинского колледжа

The authors studied the state of the indicators of the immune system in the dynamics of their first year in medical school at the female students aged 15-17 years, recognized the results of medical examination of apparently healthy. In characterizing the state of cell-mediated immunity it was revealed reduction of immune-biological resistance in healthy students, manifested by the weakening of T-and B-immunity with severe tensions in the anti-viral defense, as evidenced by the significant increase in the peripheral blood cyto-toxic T lymphocytes [CD8 (+)] and NK-cells [CD16 (+)] and a decrease in the functional activity of neutrophilic granulocytes (NG). It is important that even after staying on vacation for the new academic year, these figures do not reach the initial level and remained statistically significant lower level, although there was some tendency to normalize.
 
 Thus, the authors assume that the identified changes may involve a violation of the processes of adaptation of students caused by the restructuring of dynamic stereotypes in the transition from school to the secondary vocational education with unusual difficulties on training exercise, which may be inadequate functionality of a growing and developing organism.

Functional activity of neutrophilic granulocytes in patients with relapsing herpes

Neutrophilic granulocytes exhibit different degrees of functional activity in patients with relapsing herpetic infection in various phases of the disease. A high level of myeloperoxidase is detected in the serum over the entire course of the disease and a three-fold increase of serum lactoferrin appears at the beginning of remission.

Influence of HIV Antigens on Functional Activity of Neutrophilic Granulocytes in

The effect of nine HIV antigens, including eight synthetic peptides, on the functional activity of granulocytes was studied using the reduction of nitroblue tetrazolium test (NBT test). Some peptides partly suppressed the functional activity of granulocytes. The most pronounced suppression was caused by ImVL (HIV-1 lysate immobilized on plates for ELISA) and SP-7 (a synthetic peptide from the gp41 protein of HIV-1). The degrees to which the functional activity of granulocytes was suppressed by ImVL and SP-7 was in inverse proportion to the specific antibody concentrations. No correlation was found between the reduction in the NBT test value and the amount of CD4+, CD8+ cells on CD4/8 ratio.