Function Of Activities Research Articles

Characterization of a novel antioxidant exopolysaccharide from an intestinal-originated bacteria Bifidobacterium pseudocatenulatum Bi-OTA128

Microbial exopolysaccharides (EPSs) have attracted extensive attention for their biological functions in antioxidant activities. In this study, we characterized a novel EPS produced by Bifidobacterium pseudocatenulatum Bi-OTA128 which exhibited the highest antioxidant capacity compared to nine other ropy bacterial strains, achieving 76.50 % and 93.84 % in DPPH· and ABTS·+ scavenging activity, and ferric reducing power of 134.34 μM Fe2+. Complete genomic analysis identified an eps gene cluster involved in the EPS biosynthesis of Bi-OTA128 strain, which might be responsible for its ropy phenotype. The EPS was then isolated and purified by a DEAE-Sepharose Fast Flow column. A single elution part EPS128 was obtained with a recovery rate of 43.5 ± 1.78 % and a total carbohydrate content of 93.6 ± 0.76 %. Structural characterization showed that EPS128 comprised glucose, galactose, and rhamnose (molar ratio 4.0:1.2:1.1), featuring a putative complex backbone structure with four branched chains and an unusual acetyl group at O-2 of terminal rhamnose. Antioxidant assay in vitro indicated that EPS128 exhibited antioxidant potential with 50.52 % DPPH· and 65.40 % ABTS·+ scavenging activities, reaching 54.3 % and 70.44 % of the efficacy of standard Vitamin C at 2.0 mg/L. Furthermore, EPS128 showed protective effects against H2O2-induced oxidative stress in HepG2 cells by reducing cellular reactive oxygen species (ROS) and increasing cell viability. These findings present the first comprehensive report of an antioxidant EPS from B. pseudocatenulatum, highlighting its potential as a natural antioxidant for applications in the food industry and clinical settings.

Funerary vs. domestic vessels from the Hallstatt period. A study on ceramic vases from the Milejowice settlement and the Domasław cemetery

Clay vessels have a wide variety of functions in social activities in the Hallstatt period. In addition to food storage and processing, they were used for ritual purposes and as funerary vessels. The paper presents the results of archaeological and chromatographic studies of 31 vases from two different Hallstatt culture sites in lower Silesia (Poland). The investigations included vessels fragments from the Domasław cemetery and from the Milejowice settlement. The chromatographic analyses focused on fatty acids and biomarkers and made it possible to identify the most likely sources of substances they came into contact with during use. The c-means and hierarchical cluster analyses showed that grave vessels differed from settlement ceramics. Thus, conclusions on the diverse vessel functions could be made.

Развитие компетенций и личностных качеств педагогов – психологов начального образования для реализации программ внеурочной деятельности в условиях ФГОС НОО

The article considers the elements of the content of training future teachers-psychologists of primary education for the implementation of extracurricular activities: the theoretical and methodological basis for planning and designing extracurricular activities, the basic values, principles and functions of extracurricular activities, organizational models of extracurricular activities, the structure and content of extracurricular activities. The psychological support of the educational process, diagnosis, correction and development of the mental sphere and behavior of children, as well as counseling and support for teachers and parents, which can be carried out by a teacher – psychologist of primary education in extracurricular activities, is substantiated. The personal qualities of a specialist corresponding to the essence of extracurricular activities, its specifics, as well as ways of their formation in the process of professional training are studied.

Real time precisely tracing the fluctuations of mitochondrial SO2 in cells during ferroptosis and tissues using a mitochondrial-immobilized ratiometric fluorescent probe

Mitochondrial sulfur dioxide (SO2) plays important roles in physiological and pathological activities. Unfortunately, it is lack of a reliable tool to precisely visualize the mitochondrial SO2 and elaborate its complicated functions in various cytoactivities. Here we report a mitochondrial-immobilized fluorescent probe PM-Cl consisting of coumarin and benzyl chloride modified benzothiazole, which enables selective visualization of mitochondrial SO2via chemical immobilization. The spectral results demonstrated that probe PM-Cl could respond to SO2 with high selectivity and sensitivity. Co-localization and the fluorescence of cytolysis extraction verified the excellent mitochondrial targeting and anchoring abilities. Due to the chemical immobilization, probe PM-Cl could firmly retain into mitochondria after stimulation of carbonyl cyanide m-chlorophenyl hydrazone (CCCP) and H2O2. Significantly, a series of fluorescence images are indicative of capability for detecting the fluctuations of SO2 in mitochondria during ferroptosis. Furthermore, PM-Cl also could visualize SO2 in myocardium and muscle tissues after the stimulation of CCCP. Taken together, probe PM-Cl is a very potential molecular tool for precisely detecting mitochondrial SO2 to explore its complex functions in physiological and pathological activities.

The Beak of Eukaryotic Ribosomes: Life, Work and Miracles.

Ribosomes are not totally globular machines. Instead, they comprise prominent structural protrusions and a myriad of tentacle-like projections, which are frequently made up of ribosomal RNA expansion segments and N- or C-terminal extensions of ribosomal proteins. This is more evident in higher eukaryotic ribosomes. One of the most characteristic protrusions, present in small ribosomal subunits in all three domains of life, is the so-called beak, which is relevant for the function and regulation of the ribosome's activities. During evolution, the beak has transitioned from an all ribosomal RNA structure (helix h33 in 16S rRNA) in bacteria, to an arrangement formed by three ribosomal proteins, eS10, eS12 and eS31, and a smaller h33 ribosomal RNA in eukaryotes. In this review, we describe the different structural and functional properties of the eukaryotic beak. We discuss the state-of-the-art concerning its composition and functional significance, including other processes apparently not related to translation, and the dynamics of its assembly in yeast and human cells. Moreover, we outline the current view about the relevance of the beak's components in human diseases, especially in ribosomopathies and cancer.

Pentylenetetrazole-Induced Seizures in Wistar Male Albino Rats with Reference to Glutamate Metabolism.

Epilepsy is a common and heterogenous neurological disorder characterized by recurrent spontaneous seizures. Animal models like rats play a crucial role in finding of mechanism of epilepsy in different brain regions. i.e., cerebral cortex, cerebellum, hippocampus, and pons medulla. Glutamate is an important excitatory neurotransmitter in the central nervous system and also glutamate plays a vital role in neuronal development and memory. The process of neuronal death evolved by glutamate receptor activation, has been hypothesized in both acute and chronic degenerative disorders including epilepsy. Considering the multifactorial neurochemical and neurophysiological malfunctions consequent to epileptic seizures, a few antiepileptic drugs are designed, to mitigate the debilitating aspects of epilepsy. Rat model, pentylenetetrazole (PTZ), an anticonvulsant drug, was selected for the present study. Induction of epilepsy/convulsions was induced by an intraperitoneal injection of PTZ (60 mg/kg body weight) in saline. Biochemical assays performed through spectrophotometer. Glutamine and Glutamine synthetase levels were decreased in the epileptic rats brain regions i.e., hippocampus, cerebellum, cerebral cortex, and pons medulla; glutamate dehydrogenase and glutaminase levels were increased in all the regions of epilepsy induced rats. Highest values are recorded in hippocampus when compared to other brain regions. PTZ suppresses the function of Glutamine and Glutamine synthetase activities in selected brain regions of rat and enhances the activities of the glutaminase and glutamate dehydrogenase when compared to control rats.

Quantifying quanta: Determining emission rates from clinical data

It is important to quantify uncertainty in the viable genomic material encapsulated in the respiratory particles emitted by infected people so that it can be converted into an emission rate as a function of respiratory and metabolic activities and used to estimate the probability of infection for an indoor scenario. Clinical measurements of viral loads for SARS-CoV-2 made using infection surveys, Gesundheit-II samplers, and human challenge studies are evaluated and a mathematical model is derived to estimate the quantum emission rate as a function of the genomic and viable viral loads. Modelled emission rates for SARS-CoV-2 agree with clinical data above detection limits. The viral load is found to vary over at least 6 orders of magnitude because it is person and time dependent, and contingent on many other factors that are difficult to quantify. It is similarly large for other respiratory pathogens. Therefore, the genomic and viable-virion emission rates display similar heterogeneity. When emission rates are used to estimate absolute infection risk using the Wells-Riley model, the predictions are so uncertain that they cannot be used in any meaningful way to provide useful quantitative guidance for designing indoor spaces.

THỰC TRẠNG QUẢN LÝ CÔNG TÁC SINH VIÊN Ở CÁC TRƯỜNG ĐẠI HỌC CÔNG LẬP KHU VỰC BẮC TRUNG BỘ

The current situation of student affairs management at public universities in the North Central region has been analyzed and evaluated. The contents surveyed include: The need, contents, and factors affecting student affairs management. These contents are surveyed and analyzed according to the basic functions of management activities (planning, organizing, directing and evaluating). In addition, factors affecting student affairs management were also surveyed according to different levels of impact and influence. Survey results of officials, employees and learners at five public universities show that planning is strictly and closely implemented in effective direction, organization, and regular inspection and evaluation of student affairs management. However, there are some problems and limitations, for example: The developed plan is still scattered and lacks focus; There are still many limitations in organizational capacity and efficiency; The direction is still heavy on administration, not close to professional expertise; Inspection and evaluation have not fully promoted the inherent strength and functions...

The Important of Management Functions in Sustainable MICE

This study aims to analyze the importance of management functions in sustainable meetings, incentives, conferences, and exhibition activities at Hotel Indigo Bali Seminyak Beach. The informants in this study were the director of events, assistant director of events, and business development executive who purposively determined. Data collection is carried out through observation, literature study, interviews, group discussions, and documentation. The results reveal that sales teams managed meeting, incentive, conference, and exhibition activities have made significant strides in implementing sustainable practices in various aspects of meetings, incentives, conferences, and exhibitions. Many aspects have been successfully integrated, such as local souvenirs, adoption of renewable energy, electronic registration, and health and safety promotion still need improvement. The gap arises from the scale of events, resource limitations, and technical constraints. A comprehensive analysis of the implementation of sustainable meeting, incentive, conference, and exhibition management functions shows the establishment of strong local community ties, innovation in menu variations, prioritizing environmentally friendly practices, collaborating with local craft entrepreneurs, optimizing technology and social media, maintaining environmentally friendly commitments, and recommending the use of digital-based media in various management functions. The implementation of this management function supports improving the hotel's ability to achieve sustainable meeting, incentive, conference, and exhibition activities.

Cardiac myosin binding protein-C phosphorylation as a function of multiple protein kinase and phosphatase activities

Phosphorylation of cardiac myosin binding protein-C (cMyBP-C) is a determinant of cardiac myofilament function. Although cMyBP-C phosphorylation by various protein kinases has been extensively studied, the influence of protein phosphatases on cMyBP-C’s multiple phosphorylation sites has remained largely obscure. Here we provide a detailed biochemical characterization of cMyBP-C dephosphorylation by protein phosphatases 1 and 2 A (PP1 and PP2A), and develop an integrated kinetic model for cMyBP-C phosphorylation using data for both PP1, PP2A and various protein kinases known to phosphorylate cMyBP-C. We find strong site-specificity and a hierarchical mechanism for both phosphatases, proceeding in the opposite direction of sequential phosphorylation by potein kinase A. The model is consistent with published data from human patients and predicts complex non-linear cMyBP-C phosphorylation patterns that are validated experimentally. Our results suggest non-redundant roles for PP1 and PP2A under both physiological and heart failure conditions, and emphasize the importance of phosphatases for cMyBP-C regulation.

Item-reduced Forgotten Joint Score provides adequate psychometric properties in ACLR patients.

The purpose of this study was to evaluate content validity, test-retest reliability, internal consistency, construct validity, responsiveness and floor/ceiling effects of Forgotten Joint Score (FJS) for assessing functions in activities of daily living (ADL) following anterior cruciate ligament reconstruction (ACLR) and perform an item reduction of FJS. Swedish-speaking ACLR patients in one surgical clinic were eligible. Content validity was evaluated through patient responses and patient and expert clinician relevance ratings, omitting items with low relevance. Principal component factor analysis, Cronbach's ⍺, paired t test, correlations between FJS and Knee Injury and Osteoarthritis Outcome Score (KOOS), Cohen's d effect sizes (ESs) andstandardized response mean (SRM) were used to evaluate internal consistency, test-retest reliability, construct validity andresponsiveness. Floor/ceiling effects were calculated. FJS was expected to reveal one dominant factor, a Cronbach's ⍺ between 0.70 and 0.95, correlations >0.50 to all KOOS subscores, highest for ADL, moderate ES and SRM, floor/ceiling effects <15%. One hundred and seventy-six participants (103 for assessing internal consistency, construct validity, responsivenessand floor/ceiling effects; 73 for assessing test-retest reliabilityand content validity) were included. Item reduction yielded a nine-item FJS (FJS-9). FJS-12 and FJS-9 demonstrated sufficient content validity (95% confidence interval [CI], 2.5-3.1, respectively, 2.9-3.3). FJS-9 was found unidimensional, and FJS-12 was multidimensional. Cronbach's ⍺ was 0.94 for FJS, and the intraclass correlation coefficient > 0.90. FJS showed significant correlations >0.65 to all KOOS subscales, moderate ES and SRM > 0.50. No floor/ceiling effects were found. FJS-9 demonstrated adequate validity for the evaluation of joint awareness in ACLR patients and can be a valuable tool to assess ADL and joint awareness. Level III.

Identification of phosphorylated small ORF-encoded peptides in Hep3B cells by LC/MS/MS

Small ORF-encoded peptides (SEPs) are a class of low molecular weight proteins and peptides comprising <100 amino acids with important functions in various life activities. Although the sequence length is short, SEPs might also have post-translational modification (PTM). Phosphorylation is one of the most essential PTMs of proteins. In this work, we enriched phosphopeptides with IMAC and TiO2 materials and analyzed the phosphorylated SEPs in Hep3B cells. A total of 24 phosphorylated SEPs were identified, and 11 SEPs were coded by ncRNA. For the sequence analysis, we found that the general characteristics of phosphorylated SEPs are roughly the same as canonical proteins. Besides, two phosphorylation SEPs have the Stathmin family signature 2 motif, which can regulate the microtubule cytoskeleton. Some SEPs have domains or signal peptides, indicating their specific functions and subcellular locations. Kinase network analysis found a small number of kinases that may be a clue to the specific functions of some SEPs. However, only one-fifth of the predicted phosphorylation sites were identified by LC/MS/MS, indicating that many SEP PTMs are hidden in the dark, waiting to be uncovered and verified. This study helps expand our understanding of SEP and provides information for further SEP function investigation. SignificanceSmall ORF-encoded peptides (SEPs) are important in various life activities. Although the sequence length is short (<100AA), SEPs might also have post-translational modification (PTM). Phosphorylation is one of the most essential PTMs of proteins. We enriched phosphopeptides and analyzed the phosphorylated SEPs in Hep3B cells. That is the first time to explore the PTM of SPEs systematically. Kinase network analysis found a small number of kinases that may be a clue to the specific functions of SEPs. More SEP PTMs are hidden in the dark and waiting to be uncovered and verified. This study helps expand our understanding of SEP and provides information for further SEP function investigation.

LINGUODIDACTIC PRINCIPLES OF DEVELOPING PROFESSIONAL COMPETENCE FOR SIMULTANEOUS INTERPRETATION TEACHERS

This article identifies and describes the language teaching principles for the formation of professional competence in simultaneous interpretation teachers. An analysis of domestic and foreign theoretical and practical research reveals that the problem of developing professional competence for simultaneous interpretation teachers, despite its undeniable significance, is still in the early stages of study. In the scientific, pedagogical, and translation discourses, the search for effective solutions has only just begun. Determining the linguodidactic principles of preparing translation teachers in general and forming their professional competence provides the basis for a comprehensive study of this complex issue. It is established that the professional competence of a translation teacher is an integrative characteristic of personality, which presupposes the formation of a complex of necessary competencies for effective pedagogical and translation activities, including various components of pedagogy and translation, and pedagogical communication within the framework of intercultural communication. The professional competence of a simultaneous interpreter is a complex formation that includes both general translation competencies, reflecting the main functions of translation activities, and specific competencies necessary for oral interpretation, including the simultaneous one (as readiness and ability to perceive and understand the oral speech of communicators from different cultures, to transmit it by the communication situation, genre, style, and register; ability to immerse oneself in situations of interlingual and intercultural interaction; ability to activate necessary competencies for translation and teaching activities; readiness for enriching communicative experience while mobilizing already developed skills and abilities; capacity for reflection and self-reflection). Linguodidactic principles of developing professional competence for simultaneous interpretation teachers are defined, taking into account the interdisciplinary nature of this process, namely: pedagogical, translation studies, linguistic, and psychological principles, without which it is impossible to organize the training process for future specialists qualitatively.

TRENDS OF IMPLEMENTATION OF RESEARCH-EXPERIMENTAL ACTIVITIES IN PRESCHOOL EDUCATIONAL INSTITUTIONS OF UKRAINE IN TODAY’S CONDITIONS

The article characterizes the current state of implementation of children’s experimentation in preschool pedagogy of Ukraine. The results of a survey of pedagogues of preschool educational institutions regarding the peculiarities of the organization and implementation of the functions of research-experimental activities of preschoolers have been given. The authors carried out definitive analysis of the concepts of the research «children's experimentation», «research-experimental activity». The content and features of the organization of research-experimental activities of preschool children have been clarified, taking into account the psychological-pedagogical publications of modern foreign and Ukrainian scientists and the available creative pedagogical experience. Examples of researches and experiments for preschool children have been offered. Materials and methods. Theoretical (analysis and synthesis of scientific sources, generalization and systematization) and empirical methods (survey) have been used in the process of research. The authors conducted a questionnaire in order to find out the state of the organization of children’s experimentation in preschool educational institutions. Results. As a result of the research, it is established that almost the third of the respondents are afraid to change the traditional system of preschool education, namely the transfer of «ready-made» knowledge to children, which becomes the main obstacle to the introduction of experimentation into educational practice. Conclusions. Based on the analysis of pedagogues’ surveys, the study of theoretical and practical experience, trends of implementation of children’s experimentation in preschool educational institutions in today’s conditions have been determined.

Spiral wave dynamics in a neuronal network model

Spiral waves are spatial-temporal patterns that can emerge in different systems as heart tissues, chemical oscillators, ecological networks and the brain. These waves have been identified in the neocortex of turtles, rats, and humans, particularly during sleep-like states. Although their functions in cognitive activities remain until now poorly understood, these patterns are related to cortical activity modulation and contribute to cortical processing. In this work, ,we construct a neuronal network layer based on the spatial distribution of pyramidal neurons. Our main goal is to investigate how local connectivity and coupling strength are associated with the emergence of spiral waves. Therefore, we propose a trustworthy method capable of detecting different wave patterns, based on local and global phase order parameters. As a result, we find that the range of connection radius (R) plays a crucial role in the appearance of spiral waves. For R < 20 µm, only asynchronous activity is observed due to small number of connections. The coupling strength ( gsyn ) greatly influences the pattern transitions for higher R, where spikes and bursts firing patterns can be observed in spiral and non-spiral waves. Finally, we show that for some values of R and gsyn bistable states of wave patterns are obtained.

A Facile Solution Phase Synthesis of Peptide Assemblies as Functional Moiety for Antimicrobial and Anticancer Peptides

In recent years, human being are affected by various types of cancer and are highly microbial infected due to changes in environmental conditions and hence, the recovery from this cause of disease using peptide-based drugs as a function of both activities like antimicrobial peptides (AMPs) and anticancer peptides (ACPs). Herein, we report the solution phase peptide synthesis using α-amino acids such as alanine, phenylalanine and lysine. The structural parameters of the polypeptides were investigated by circular dichroism, FT-IR, MALDI-TOF ESI-mass, NMR, etc. Water-soluble hydrophilic-based synthesized polypeptides possess various biological applications throughout, especially antibacterial and anticancer activities–short reaction process, less cost of chemicals, minimum glassware and no hazardous chemicals involved. Multiple species have innate immune systems that contain antimicrobial peptides (AMPs). Most of them have been proved to possess dual action, both as antibacterial and anticancer peptides, making them most promising alternative to conventional compounds used today to treat these infections. Present approach offers unique opportunities for developing highly potent and focused antibacterial and anticancer peptide-based drugs.

"I Want to Do Something" - Exploring What Makes Activities Meaningful for Community-Dwelling People Living With Dementia: A Focused Ethnographic Study.

Supporting ageing in place, quality of life, and activity engagement are public health priorities for people with dementia. The importance of maintaining opportunities for meaningful activities has been widely acknowledged for those with dementia in long-term care, but little is known about what makes activities meaningful for, and how they are experienced by, people with different types of dementia in their own homes. This study used focussed ethnographic methods to explore the motivations and meanings of everyday activity engagement within the homes of 10 people with memory-led Alzheimer's disease and 10 people with posterior cortical atrophy. While participants' interactions with their everyday environments were challenged by their diagnoses, they were all finding ways to continue meaning-making via various activities. The main findings are encapsulated in three themes: (1) The fun and the function of activities; (2) Reciprocities of care, and (3) The constitution and continuity of (a changing) self. Ongoing engagement with both fun and functional activities offered participants living with different dementias opportunities to connect with others, to offer care and support (as well as receive it), and to maintain a sense of self and identity. Implications are discussed regarding the development and delivery of tailored interventions and support to enable continued engagement in meaningful activities for people with different types of dementia living in the community.

Neurooncological Rehabilitation in Diffuse Gliomas.

With the further advancement of surgical technology and modern tumor-targeted treatment strategies, longer survival rates can be achieved in diffuse gliomas. Pre- and post-therapeutic physical and cognitive deficits are frequently associated with gliomas. The clinical impact of physical therapy and rehabilitation on neurooncological disorders has not been analyzed consecutively. This study investigates the clinical effect of rehabilitation in patients with diffuse gliomas. Patients with surgically and radio-/oncologically treated diffuse gliomas were recruited into this study. They were admitted to an inpatient program for three weeks. The patients underwent physical and occupational therapy, exercise programs, and psychooncological support. The outcome measures included motor strength, mobility, neuropsychological deficits, and tumor localization-dependent symptoms for the statistical determination and comparison of the respective Eastern Cooperative Oncology Group (ECOG) scores on admission and discharge by a two-tailed t-test. A total of 25 patients (f/m: 11/14) with diffuse gliomas were recruited into the program. Four patients (16%) had CNS WHO grade 2, seven patients (28%) had grade 3, and 14 patients (56%) had grade 4 tumors. Major improvement in motor, cognitive, and functions of the daily activities was achieved in the examined population.Major improvement in motor, cognitive, and neurological functions of the daily activities screened in the admission of all patients was achieved. The comparison of the ECOG scores determined on admission and on discharge showed a statistical significance derived from the undertaken t-test with a p-value <0.05. We herein show that a clearly defined inpatient rehabilitation protocol significantly enables the improvement of the quality of life of patients with diffuse gliomas. The effectiveness of the exercise program and psychooncological assistance was confirmed by the course of patient-reported functions. Based on the limited number of our patient collective, multicenter studies with broader patient sizes should be performed to confirm our significant results.

Microvascular Perfusion Imaging in Alzheimer's Disease.

Alzheimer's disease (AD) is the leading cause of dementia worldwide and significantly impacts the essential functions of daily life and social activities. Research on AD has found that its pathogenesis is related to the extracellular accumulation of amyloid-beta (Aβ) plaques and intracellular neurofibrillary tangles in the cortical and limbic areas of the human brain, as well as cerebrovascular factors. The detection of Aβ or tau can be performed using various probes and methodologies. However, these modalities are expensive to implement and often require invasive procedures, limiting accessibility on a large scale. While magnetic resonance imaging (MRI) and computed tomography (CT) are generally used for morphological and structural brain imaging, they show wide variability in their accuracy for the clinical diagnosis of AD. Several novel imaging modalities have emerged as alternatives that can accurately and vividly display the changes in blood flow and metabolism in each brain area and enable physicians and researchers to gain insights into the generation and progression of the cerebro-microvascular pathologies of AD. In this review, we summarize the current knowledge on microvascular perfusion imaging modalities and their application in AD, including MRI (dynamic susceptibility contrast-MRI, arterial spin labeling-MRI), CT (cerebral CT perfusion imaging), emission computed tomography (positron emission tomography (PET), single-photon emission computed tomography (SPECT)), transcranial doppler ultrasonography (TCD), and retinal microvascular imaging (optical coherence tomography imaging, computer-assisted methods for evaluating retinal vasculature).

Russia Abroad vs Russian Diaspora in the Interwar Period

This article considers a relevant issue of modern historiography, i. e. the expediency of introducing the concept of “Russia Abroad” in the study of Russian emigration of the 1920s–1930s, along with the definition of “diaspora”. The task is complicated by the lack of a unified categorical apparatus and a variety of interpretations. The problem is solved based on structural and constructivist approaches, as well as comparative analysis. The authors propose their own classification of identifying features of the diaspora, highlighting the geographical (territorial), social, political, cultural, and mental blocks. The phenomenon of Russia Abroad is analysed through the prism of diasporisation. The article shows the process of institutionalisation of the Russian emigration of the 1920s, which brings it closer to the diaspora. However, its composition, unlike that of diaspora, was polyethnic. The dominant trend was the preservation of not ethnic but cultural and civic identity, which determined the structure-forming leitmotif of activity aimed at constructing its “Russia Abroad”. However, having renounced the citizenship of Soviet Russia/USSR and recognising the Soviet government as illegitimate, the emigrants unsuccessfully tried to consolidate a legal connection with the state that had disappeared from the political map of the world, trying to defend the old passports. The vectors of reflexive activity with a myth-generating potential were an ostentatious opposition to all things Soviet, the preservation of national identity and traditional values (unlike those born in the revolution), the “ethical code”, social norms, and the justification of the missionary role of emigration. Of the many institutions that claimed to have become a successor authority, the Council of Former Ambassadors headed by M. N. Girs stands out. He concentrated human, financial, material, and organisational potential in his hands and had representation at international organisations, including the League of Nations, in Geneva. The foreign diplomatic corps became the guardian of the idea of all-Russian statehood. Well-established communication links helped him to carry out tasks that were not typical of him, adapted to the new situation and focusing primarily on the settlement of the legal situation of refugees. The article concludes about the consolidating function of both mythmaking and activities aimed at reproducing the attributes of statehood. The Russian “state-like form of being” contributed to the preservation of the Russian language, national identity, and the creation of a system of Russian educational institutions and institutes of historical memory. The doom of Russia Abroad was due to both its conservatism and confrontation with the motherland. The division into two Russias could not carry a positive potential. Foreign Russia can be characterised as a diaspora, but this phenomenon goes beyond even the wide range of identification features that are given to the diaspora today.