Absorptive Function Research Articles

New insights into the role of the root system of epiphytic bromeliads: comparison of root and leaf trichome functions in acquisition of water and nutrients.

In epiphytic bromeliads, the roots used to be considered poorly functional organs in the processes of absorption and metabolization of water and nutrients, while the leaves always acted as protagonists in both functions. More recent discoveries have been changing this old view of the root system. In this review, we will address the old thoughts of the scientific community regarding the function performed by the roots of epiphytic bromeliads (mere holdfast structures with low physiological activity) and the importance of a reduced or lack of root system for the emergence of epiphytism. We will present indirect and direct evidence that contradicts this older hypothesis. Furthermore, the importance of the root absorptive function mainly for juvenile tankless epiphytic bromeliads and the characteristics of the root absorption process of adult epiphytic tank bromeliads will be thoroughly discussed in physiological aspects. Finally, some factors (species, substrate, environmental conditions) that influence the absorptive capability of the roots of epiphytic tank bromeliads will also be considered in this review, highlighting the importance that the absorptive role of the roots have for the plasticity of bromeliads that live on trees, which is an environment characterized by the intermittent availability of water and nutrients. The roots of tank-forming epiphytic bromeliads play important roles in the absorption and metabolization of nutrients and water. The importance of roots stands out mainly for juvenile tankless bromeliads since the root is the main absorptive organ. In larger plants with tank, although the leaves become the protagonists in the resource acquisition process, the roots complement the absorptive function of the leaf trichomes, resulting in a better growth of the bromeliad. The physiological and biochemical properties of the processes of absorption and distribution of resources in the tissues seem to differ between absorption by trichomes and roots.

The fate of secretory cells during intestinal homeostasis, regeneration, and tumor formation is regulated by Tcf4.

The single-layer epithelium of the gastrointestinal tract is a dynamically renewing tissue that ensures nutrient absorption, secretory and barrier functions and is involved in immune responses. The basis for this homeostatic renewal is the Wnt signaling pathway. Blocking this pathway can lead to epithelial damage, while its abnormal activation can result in the development of intestinal tumors. In this study, we investigated the dynamics of intestinal epithelial cells and tumorigenesis using a conditional mouse model. Using single-cell and bulk RNA sequencing and histological analysis, we elucidated the cellular responses following the loss of specific cell types. We focused on the fate of cells in the lower parts of the intestinal crypts and the development of colon adenomas. By partially inactivating the transcription factor Tcf4, a key effector of the Wnt signaling pathway, we analyzed the regeneration of isolated hyperproliferative foci (crypts). Our results suggest that the damaged epithelium is not restored by a specific regeneration program associated with oncofetal gene production, but rather by a standard homeostatic renewal pathway. Moreover, disruption of Tcf4 in secretory progenitors resulted in a significant shift in the cell lineage from Paneth cells to goblet cells, characterized by morphological changes and loss of Paneth cell-specific genes. We also found that hyperactivation of the Wnt signaling pathway in colonic adenomas correlated with the upregulation of genes typical of Paneth cells in the intestine, followed by the emergence of secretory tumor cells producing the Wnt3 ligand. The absence of Tcf4 led to a phenotypic shift of the tumor cells towards goblet cells. Our study presents a new model of epithelial regeneration based on the genetically driven partial elimination of intestinal crypts. We highlight the critical role of Tcf4 in the control of cell lineage decisions in the intestinal epithelium and colon tumors.

Spline-based Abel Transform (SAT) radial property reconstruction for noise and trapping correction: Application to axisymmetric sooting flames

In combustion research, optical diagnostics often necessitate Abel inversion to deconvolve the measurements of axisymmetric flames, especially to determine local soot temperature fields from integrated flame radiative emission measurements. However, traditional Abel inversion methods can considerably amplify experimental noise, especially towards the flame centerline. Although various strategies exist to mitigate this issue, they typically do not address the correction of signal trapping within the flame. The present paper introduces an adapted Abel inversion tool that combines resistance to experimental noise with a mechanism to correct signal trapping effects. The tool employs noise-free Abel inversion using piecewise spline functions. Its effectiveness is validated through numerical comparisons with conventional methods. The practical application is demonstrated in the measurement of soot temperature in a canonical laminar diffusion ethylene flame. This is achieved by using multi-wavelength line-of-sight attenuation (LOSA) and emission measurements. The tool corrects for the signal trapping effect in emission data by integrating extinction profiles, thus enabling a more precise soot temperature analysis. The current study also compares two techniques of temperature measurement based on thermal emission: traditional two-color pyrometry and a calibrated emission and absorption ratio method. The study further explores the effects of signal trapping, scattering contribution, and the absorption function ratio on soot temperature determination based on experimental profiles. The findings suggest that signal trapping and scattering contributions have a negligible impact on measurements at the wavelengths examined. However, the choice of absorption function ratio significantly influences the outcomes of two-color pyrometry, highlighting the advantage of the one color emission/absorption technique for the determination of sooting flames temperature measurements.

A systematic first-principles investigation of the structural, electronic, mechanical, optical, and thermodynamic properties of Half-Heusler ANiX (ASc, Ti, Y, Zr, Hf; XBi, Sn) for spintronics and optoelectronics applications.

This paper is the first to look at the structural, electronic, mechanical, optical, and thermodynamic properties of the ANiX (ASc, Ti, Y, Zr, Hf; XBi, Sn) half-Heusler (HH) using DFT based first principles method. The lattice parameters that we have calculated are very similar to those obtained in prior investigations with theoretical and experimental data. The positive phonon dispersion curve confirm the dynamical stability of ANiX (ASc, Ti, Y, Zr, Hf; XBi, Sn). The electronic band structure and DOS confirmed that the studied materials ANiX (ASc, Ti, Y, Zr, Hf; XBi, Sn) are direct band gap semiconductors. The investigation also determined significant constants, including dielectric function, absorption, conductivity, reflectivity, refractive index, and loss function. These optical observations unveiled our compounds potential utilization in various electronic and optoelectronic device applications. The elastic constants were used to fulfill the Born criteria, confirming the mechanical stability and ductility of the solids ANiX (ASc, Ti, Y, Zr, Hf; XBi, Sn). The calculated elastic modulus revealed that our studied compounds are elastically anisotropic. Moreover, ANiX (ASc, Ti, Y, Zr, Hf; XBi, Sn) has a very low minimum thermal conductivity (Kmin), and a low Debye temperature (θD), which indicating their appropriateness for utilization in thermal barrier coating (TBC) applications. The Helmholtz free energy (F), internal energy (E), entropy (S), and specific heat capacity (Cv) are determined by calculations derived from the phonon density of states.

Efficacy & Significance of LYSO HERB Herbal Lysine in the nutrition, metabolism and health in Poultry & Efficacy and significance of the phytogenic METHO ADD Herbal Methionine as replacer of Synthetic DL- Methionine in nutrition and health of Poultry

In poultry industry, amino acids are supplied along with vitamins and minerals since these play a pivotal role as protein building units which are the essentialities of growth and metabolism. Of the 9 essential amino acids Methionine is the first limiting one followed by Lysine, these cannot be synthesized by animals hence to be obtained from external sources majorly the feed. Amino acids act as intermediates in all the metabolic and physiological functions of the bird like calcium absorption, production of collagen, antibodies, hormones, enzymes. Their deficiency causes de-pigmentation, reduced hemoglobin, poor FCR, reduced production, retarded growth, poor feathering and egg production, immune suppression and increased disease incidence. Cells in the body are constantly being broken down and restored, so the body needs these amino acids to produce new hormones and restore the body. Of late the use of synthetic Lysine and Methionine as supplement has increased due to high demand of meat. The safety of these is questionable due to higher pricing, emergence of drug resistance, residual toxicity and other side effects. Therefore, a renewed interest in developing natural alternatives to maintain production, performance and health in poultry, hence Phytogenic herbals are considered the best option.. The results obtained here provide clear evidence of the potential of Phytogenic Herbal Lysine as Lyso Herb and Herbal Methionine as Metho Add as substitute of synthetic Lysine & Methionine with unaltered activity throughout, without reducing growth performance, increasing fat accretion and changing serum biochemical characteristics, with better performance and economics.

HIGH-QUALITY CASSAVA PEEL MEAL FOR GROWING PIGS: IMPLICATIONS ON CARCASS, MEAT QUALITY, ORGAN WEIGHTS, HEPATIC AND JEJUNUM HISTOLOGY

The global increase in the production and processing of cassava tubers generates a lot of peel as waste. However, the effect of processing methods to increase the quality of the peel as an animal feed resource must look at the quality of the resultant food products. These effects are insufficiently studied. The study aimed to assess the impact of the processed peel on carcass quality, meat quality, organ weight and organ histology of growing pigs. Thirty (30) male growing pigs (Landrace × Large white) were randomly distributed to five groups with six (6) animals per group in a completely randomized design. Maize was substituted with high quality cassava peel meal (HQCPM) at 0%, 25%, 50%, 75%, and 100% for the experimental diets ranging from diets V to Z. The pigs fed HQCPM diets had higher carcass weights, resulting in an improved carcass yield. The colour, flavour, tenderness, juiciness, texture, and overall acceptability were statistically superior in the pigs fed diet Z. High feed fibre influenced organ weights: the HQCPM diets reduced (P<0.05) the organ weights. More so, the HQCPM diets affected (P<0.05) the histology of the liver and intestine with varying degrees of inflammation, shortened villi length, and reduced surface area. HQCPM diets enhanced the carcass weight and meat quality of growing pigs up to 100% replacement for maize. Nevertheless, the diets reduced organ weights and villi lengths, leading to reduced absorptive function.

Frequency selective surface with quad-band absorption/transmission and angular stability for TE and TM polarizations

The proposed FSS structure employs two square annular rings for absorption functionalities and two square aperture slots for transmission properties. Through comprehensive simulations and analysis, the FSS structure demonstrates remarkable performance in selectively absorbing and transmitting electromagnetic waves across four distinct frequency bands. Moreover, the design exhibits excellent TE and TM polarizations angular stability, consistently maintaining constant absorption and transmission characteristics across varying incidence angles. A comprehensive analysis of the equivalent circuit model of the FSS structure has also been performed to understand the quad-band transmission and absorption properties. The measurement results underscore the effectiveness of the design of a 250*250 mm array, with absorption peaks notably occurring at 4.2 GHz and 7.23 GHz, boasting peak absorption rates of 92% and 85%, respectively. Moreover, the insertion loss within the pass band at 8.8 GHz and 12.2 GHz stands impressively low at 0.2 dB and 0.32 dB, respectively. Notably, the proposed quad-band FSS showcases polarization-insensitive behavior across a range from 0° to 60° and successfully undergoes conformal testing with a curvature radius up to 50 mm. The results of experimental testing on a quad-band FSS prototype demonstrate superior agreement with those obtained through simulations and analytically derived equivalent circuit model (ECM) results, validating its practical applicability in real-world scenarios.

Hollow engineering of Co/N-doped C@carbon aerogel with hierarchical structure boosting interfacial polarization for ultra-thin microwave absorption and thermal insulation

Rational manipulation of the composition and microstructure design of aerogel materials is considered to be an effective method for achieving the dual functions of microwave absorption and thermal insulation. Herein, hollow Co/N-doped C@carbon aerogel with hierarchical structures was designed and constructed via a synergistic strategy including directed freeze-drying, ZIF-67 in situ growth, chemical etching and calcination treatment. Co/N-doped hollow carbon nanocages derived from ZIF-67 with uniform heterojunctions and layered micro-mesopores were constructed using tannic acid and heat treatment process. After that, abundant macroporous structure from the carbon aerogel itself was further introduced to upgrade the impedance matching property, light weight and interfacial polarization loss capability. Remarkably, as-fabricated Co/N-doped C@carbon aerogel (ZTA@A-900) displays optimal MA performance with a minimum reflection loss (RLmin) value of −54.0 dB at 15.32 GHz and a broad effective absorption bandwidth (EAB) of 4.04 GHz (13.44–17.48 GHz) at a thickness of only 1.4 mm. Furthermore, the ideal CST simulation results and excellent thermal insulation property of ZTA@A-900 make it an outstanding candidate for microwave absorbers under extreme conditions. Thus, our work opens up a new avenue for the design and fabrication of multifunctional lightweight microwave absorbers.

A wood-inspired epoxy-based electronic packaging material with vertically aligned thermally conductive channels for electromagnetic waves absorption and thermal management

Multifunctional materials integrating electromagnetic waves absorption (EMA) and heat conductivity functions are urgently desirable to tackle the heat emission and electromagnetic interference issues of advanced electronics. However, simultaneously mustering these features into one material remains a formidable challenge as the incompatibility between high thermal conductivity and excellent EMA performance. Herein, inspired by the water transport process of trees in nature, an epoxy-based electronic packaging material (CFS/EB) with vertical thermal conduction channels is fabricated by encapsulating high thermal conductivity boron nitride/epoxy hybrids into aerogel skeleton with good electromagnetic features. This ingenious design not only can defuse the issue of difficult coexistence of outstanding EMA and thermal conduction performance, but also delivers high through-plane thermal conductivity arised from vertical aligned channels. The resulting CFS/EB composite exhibits strong EMA performance with minimum reflection loss value of −59.2 dB and broad effective absorption bandwidth of 5.82 GHz. Synchronously, the composite also holds the high through-plane thermal conductivity of 1.51 W m−1 K−1 (655 % higher than pure epoxy resin) at low filler loading of 13.15 wt% as well as electrical insulation of 1.43 × 1012 Ω·cm. Such a superior comprehensive performance endows CFS/EB composite a great application prospects in electronic packaging fields, whilst the orientation structure design triggered by this bioinspired strategy offer facile yet feasible alternatives to address heat accumulation and electromagnetic interference problems for integrated electronics.

Switchable bi-functional water-based metasurface for high efficiency and wideband polarization conversion and absorption

A reconfigurable metasurface with a switchable function, wideband, and high efficiency has gained a lot of attention for the creation of compact and efficient devices. Here, for the first time, we propose a bi-functional metasurface that utilizes a water-based resonator to achieve wideband and high-efficiency absorption and cross polarization conversion in the microwave range. By controlling the water injection, the functionality of the metasurface can switch between absorption and cross-polarized conversion. Via water injection, the designed water-based metasurface can act as a wideband absorber with an absorptivity of above 90% in the broadband range of 16.5–24 GHz. Meanwhile, without water injection, it exhibits the ability for high efficiency cross-polarization conversion across a wideband in the range of 4.38–11.9 GHz. The proposed metasurface is fabricated and measured to verify its ability to switch between the dual functions of high efficiency wideband absorption and cross-polarization conversion. We believe that the metasurface platform shown here will open up new possibilities for creating compact multifunctional devices for applications in the microwave region.

Microplastics enhanced the allelopathy of pyrogallol on toxic Microcystis with additional risks: Microcystins release and greenhouse gases emissions

Cyanobacteria blooms (CBs) caused by eutrophication pose a global concern, especially Microcystis aeruginosa (M. aeruginosa), which could release harmful microcystins (MCs). The impact of microplastics (MPs) on allelopathy in freshwater environments is not well understood. This study examined the joint effect of adding polystyrene (PS-MPs) as representative MPs and two concentrations (2 and 8 mg/L) of pyrogallol (PYR) on the allelopathy of M. aeruginosa. The results showed that the addition of PS-MPs intensified the inhibitory effect of 8 mg/L PYR on the growth and photosynthesis of M. aeruginosa. After a 7-day incubation period, the cell density decreased to 69.7 %, and the chl-a content decreased to 48 % compared to the condition without PS-MPs (p < 0.05). Although the growth and photosynthesis of toxic Microcystis decreased with the addition of PS-MPs, the addition of PS-MPs significantly resulted in a 3.49-fold increase in intracellular MCs and a 1.10-fold increase in extracellular MCs (p < 0.05). Additionally, the emission rates of greenhouse gases (GHGs) (carbon dioxide, nitrous oxide and methane) increased by 2.66, 2.23 and 2.17-fold, respectively (p < 0.05). In addition, transcriptomic analysis showed that the addition of PS-MPs led to the dysregulation of gene expression related to DNA synthesis, membrane function, enzyme activity, stimulus detection, MCs release and GHGs emissions in M. aeruginosa. PYR and PS-MPs triggered ROS-induced membrane damage and disrupted photosynthesis in algae, leading to increased MCs and GHG emissions. PS-MPs accumulation exacerbated this issue by impeding light absorption and membrane function, further heightening the release of MCs and GHGs emissions. Therefore, PS-MPs exhibited a synergistic effect with PYR in inhibiting the growth and photosynthesis of M. aeruginosa, resulting in additional risks such as MCs release and GHGs emissions. These results provide valuable insights for the ecological risk assessment and control of algae bloom in freshwater ecosystems.

Functional insights of digestion, absorption, and immunity in different segments of the intestine in Hemibarbus labeo from transcriptomic analysis

The intestine is an important organ for food digestion and absorption and body immunity in fish. In this study, we investigated the abundance of transcripts from different segments of the intestinal tract using transcriptome sequencing technology in Hemibarbus labeo, to provide functional insights into digestion, absorption, and immunity in the anterior intestine (AI), middle intestine (MI), and posterior intestine (PI). We found 5646 differentially expressed genes (DEGs), which were significantly enriched to GO terms of carbohydrate metabolic process, transmembrane transport, iron ion binding, lipid metabolic process, and KEGG pathway of fat digestion and absorption, mineral absorption, protein digestion and absorption, vitamin digestion and absorption, indicating that the digestion and absorption function of food is different in AI, MI, and PI. In practice, most genes, enriched in the KEGG pathway for digestion and absorption of nutrients, are upregulated in AI and MI, indicating stronger roles for food digestion and absorption in these segments. Furthermore, we found that genes involved in the KEGG pathway of lysosome and endocytosis pathway are upregulated in PI, suggesting stronger antigen-presenting capabilities in PI. However, some cytokine receptor genes, including ccr4, cxcr2, tnfrsf9, il6r, csf3r, and cxcr4, are highly expressed in AI, reflecting the regional immune specialization in different segments. This study provides functional insights into digestion, absorption, and immunity in different segments of the intestine and supports the regional functional specialization within different segments of the intestine in H. labeo.

Microplastics weaken the digestion and absorption functions in the golden pompano (Trachinotus blochii) by affecting the intestinal structure, bacteria and metabolites

Microplastics are difficult to degrade and widespread environmental pollutants. Coastal areas are hardest hit of microplastic pollution as they receive significant amounts of microplastics discharged from inland sources. Golden pompano (Trachinotus blochii) is a high commercial valuable marine aquaculture fish species, most of the golden pompano are raised in coastal areas, which means they are at significant risk of exposure to microplastics. Therefore, we exposed golden pompano to 10 μg/L, 100 μg/L and 1000 μg/L of 5 μm spherical polystyrene microplastics and conducted a 14-day stress experiment. Histopathology results showed the intestinal villi shrank. The 16s sequencing analysis revealed that microplastics significantly impacted the abundance and community structure of intestinal microorganisms, which may affect the metabolic function of the gastrointestinal tract. Metabolomics sequencing of the intestinal contents showed that microplastics caused disruptions in lipid, glucose, and amino acid metabolism, thus compromising the normal digestion and absorption functions in the intestinal system. In addition, the activation of various pathways, including the intestinal endocrine system, proline metabolism, and signal transduction, which can lead to the occurrence of several diseases. This study combined various methods to investigate the adverse effects of microplastics on intestinal digestion and absorption, and provided new insights into the toxic mechanisms of microplastics.

The structural, magnetic, optoelectronic, and mechanical characteristics of NaGeX3 perovskites under pressure for soler-cell applications

This study examines the physical properties of germanium-based halide perovskite through Density Functional Theory (DFT) computations. The physical, optical, mechanical, and magnetic properties of NaGeX3 (X = Cl, Br, and I) were examined with the effects of hydrostatic pressure applied externally. The compounds were subjected to pressure variations ranging from 0 to 5 GPa. The results indicate a decrease in the band gap from the infrared to the visible spectrum. For NaGeCl3, NaGeBr3, and NaGeI3 the band gap decreased from 0.766 eV, 0.497 eV, and 0.400 eV to 0 eV, respectively, indicating the metallic behavior. The mechanical properties of NaGeX3 (X = Cl, Br, and I) demonstrate that for all three compounds, Bulk Modulus (B), Shear Modulus (G), Young’s Modulus (E), Poisson’s ratio (ν), and Pugh’s ratio B/G all increase with increasing pressure. It demonstrates that all these NaGeX3 (X = Cl, Br, I) compounds are ductile in nature. The compounds are determined to be diamagnetic based on their magnetic property investigation, which reveals no notable changes in behavior up to 5 GPa of rising pressure. To gain a better understanding of the properties of the material when incident light strikes its surface, researchers also looked in at optical absorption, reflectivity, dielectric constants, refractive index, conductivity, and loss functions. Pressure-induced NaGeX3 perovskite compounds, where X = Cl, Br, and I, show an increase in dielectric constant as pressure rises, suggesting a decrease in charge carrier recombination rates and a possibility for higher optoelectronic device efficiency. For all NaGeX3 compounds (where X = Cl, Br, and I), the maximum absorption coefficient peaks are located around 3 eV, indicating that increasing pressure increases optical conductivity. Additionally, they have significantly low reflectance throughout the visible spectrum and very narrow band gap, which indicates significant absorption and the possibility of effective Near-Infrared (NIR) Sensors, photodetector etc applications.

First-principals research to mechanical, electronic, thermodynamic, and optical properties of Al–Cu intermetallics

In this study, the mechanical, electronic, thermodynamic, and optical properties of Al2Cu, AlCu, and Al4Cu9 were investigated. The analysis of the elastic constant Cij revealed that Al2Cu, AlCu, and Al4Cu9 are mechanically stable. AlCu is a brittle material, while Al2Cu and Al4Cu9 are ductile materials. Electronic properties calculation revealed that three Al–Cu intermetallics are all conductors, and the bonding of three Al–Cu intermetallics exhibit covalent character. The effects of changes in temperature and pressure on heat capacity, thermal expansion coefficient, Debye temperature, and Grüneisen parameters were studied using quasi-harmonic Debye model. The optical properties of Al–Cu intermetallic compounds, including dielectric functions, reflectivity, absorption and loss function also be studied.

Changes in functional characteristics of heel fat pad with age

BackgroundThis study aimed to investigate age-related changes in the heel fat pad's microchamber and macrochamber layers, particularly focusing on load-induced alterations. Understanding these changes is crucial for elucidating age-related differences in foot mechanics and their potential implications for mobility and comfort. MethodsFifty-five healthy individuals were divided into three age groups: young adults (≤29 years), middle-aged adults (30–44 years), and elderly individuals (≥45 years). Ultrasonic imaging was utilized to measure the thickness of the heel fat pad's microchamber and macrochamber layers under varying load conditions. Thickness, percentage changes, and ratios of load-induced thickness changes were calculated to assess age-related differences. FindingsUnder no-load conditions, both microchamber and macrochamber layers of the heel fat pad were significantly thicker in middle-aged and elderly individuals than in young adults. When load was applied middle-aged and elderly participants exhibited smaller changes in the macrochamber layer and larger changes in the microchamber layer compared to young adults. InterpretationOur findings suggest that age influences the structural characteristics and response of the heel fat pad to mechanical loading. Thicker heel fat pad layers in middle-aged and elderly individuals under no-load conditions may reflect age-related changes in fat distribution or composition. Moreover, differences in load-induced thickness changes indicate altered mechanical properties with age, potentially affecting shock absorption and overall foot function. Understanding these age-related variations can help develop interventions aimed at preserving foot health and mobility across the lifespan.

Temporal variation in production performance, biochemical and oxidative stress markers, and gut microbiota in Pekin ducks during the late growth stage

In the late growth stage of commercial Pekin ducks, a significant increase in feed intake and a decline in body weight gain have been observed, leading to impaired feed conversion efficiency. To address this issue, we investigated alterations in production performance, blood biochemical indices, ileum tissue architecture, and microbial community structure in Pekin ducks. The primary objective was to provide robust data supporting the improvement of meat duck production efficiency during the late growth stage (28-42-days-old). Forty 28-day-old Pekin ducks were randomly assigned to 8 replicates, with five ducks per replicate. The rearing period lasted 14 days, with feed and water provided ad libitum. Our findings indicated a significant increase in Pekin duck body and heart weights with advancing age (P < 0.05). Moreover, serum antioxidant enzyme and high-density lipoprotein concentrations significantly increased, whereas triglyceride levels decreased (P < 0.05). Notably, the height of the ileal villi was significantly reduced (P < 0.05). The microbial community structure of the ileum exhibited significant changes as ducks aged, accompanied by a substantial increase in microbial flora diversity, particularly with the formation of more tightly connected microbial network modules. Time-dependent enrichment was observed in microbial gene functions related to energy metabolism pathways. At the genus level, Sphingomonas and Subdoligranulum have emerged as crucial players in microbial differential functional pathways and network formation. These bacteria likely serve as the key driving factors in the dynamic microbial changes that occur in Pekin ducks over time. Overall, our findings suggest a potential decline in the absorption function of the small intestine and fat deposition performance of Pekin ducks during later growth stages, which may be attributed to the maturation and proliferation of the gut microbial community.

Effects of alpha-lipoic acid on the growth performance and serum biochemical index values of puppies

We investigated the effects of alpha-lipoic acid (α-LA) on the growth performance and health level of puppies. A total of 16 healthy Chinese rural dogs aged 30–45 days were randomly divided into four groups: control group (CG), low-dose group (LDG), middle-dose group (MDG) and high-dose group (HDG). The puppies were fed fresh food supplemented with 0, 0.2, 0.4 and 0.6 g/kg of α-LA, respectively. Average daily feed intake (ADFI), average daily gain (ADG), feed-to-gain ratio (F/G), faecal score, intestinal absorption function and serological indices on days 1, 14 and 28 were determined and analysed. The trial period was 28 days. The following results were obtained: (1) α-LA supplementation at 0.4 and 0.6 g/kg significantly increased the ADFI and ADG of the puppies (P < 0.05) but had no significant effect on the faecal score (P > 0.05); (2) α-LA in the fresh diet significantly increased crude fat (EE) digestibility (P< 0.05) but significantly decreased calcium (Ca) digestibility (P< 0.05), without significant impacts on the digestibility of nutrients such as crude protein (CP) and total phosphorus (P); (3) with increasing α-LA inclusion levels, the content of D-xylose in the blood serum increased, but there was no significant difference among groups (P = 0.097). Adding 0.6 mg/kg α-LA to the fresh diet significantly increased the content of IgG in the serum after 14 and 28 days (P < 0.05). At 28 days, adding 0.4 and 0.6 g/kg α-LA to the fresh diet could significantly increase the contents of albumin (ALB) and total protein (TP) in the blood serum (P < 0.05), and the addition of 0.6 g/kg α-LA could significantly increase the serum levels of IgA and IgG (P < 0.05). The addition of α-LA significantly increased the activities of total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-Px) and total superoxide dismutase (T-SOD) (P < 0.05), decreased the content of malondialdehyde (MDA) (P < 0.05), decreased the levels of interleukin-1β (IL-1β) and interleukin-6 (IL-6) (P< 0.05) and increased the content of interleukin-10 (IL-10) in the blood serum (P < 0.05). In conclusion, the addition of α-LA at 0.6 g/kg to the fresh diet of puppies can improve the ADFI, ADG, serum immune capacity, antioxidant capacity and anti-inflammatory capacity of puppies.

Heat stress upregulates arachidonic acid to trigger autophagy in sertoli cells via dysfunctional mitochondrial respiratory chain function

As a key factor in determining testis size and sperm number, sertoli cells (SCs) play a crucial role in male infertility. Heat stress (HS) reduces SCs counts, negatively impacting nutrient transport and supply to germ cells, and leading to spermatogenesis failure in humans and animals. However, how HS affects the number of SCs remains unclear. We hypothesized that changes in SC metabolism contribute to the adverse effects of HS. In this study, we first observed an upregulation of arachidonic acid (AA), an unsaturated fatty acid after HS exposure by LC-MS/MS metabolome detection. By increasing ROS levels, expression of KEAP1 and NRF2 proteins as well as LC3 and LAMP2, 100 µM AA induced autophagy in SCs by activating oxidative stress (OS). We observed adverse effects of AA on mitochondria under HS with a decrease of mitochondrial number and an increase of mitochondrial membrane potential (MMP). We also found that AA alternated the oxygen transport and absorption function of mitochondria by increasing glycolysis flux and decreasing oxygen consumption rate as well as the expression of mitochondrial electron transport chain (ETC) proteins Complex I, II, V. However, pretreatment with 5 mM NAC (ROS inhibitor) and 2 µM Rotenone (mitochondrial ETC inhibitor) reversed the autophagy induced by AA. In summary, AA modulates autophagy in SCs during HS by disrupting mitochondrial ETC function, inferring that the release of AA is a switch-like response, and providing insight into the underlying mechanism of high temperatures causing male infertility.

Effects of Tannin Supplementation in Diet on the Resistance to Ammonia Stress of Pacific White Shrimp Litopenaeus vannamei

Tannin (TA), as a natural phenolic compound with strong antioxidant activity, has been used as a feed additive for various animals. In this study, we fed a diet containing 800 mg/kg of tannin on Litopenaeus vannamei for 56 days and then subjected to acute ammonia stress for 48 hr to investigate the effect of dietary tannin on the ammonia stress response of L. vannamei through transcriptomic and metabolomic analysis. The transcriptome analysis indicated that ammonia stress-induced differential expression of 4,185 genes, while tannin-fed shrimp only had 964 differentially expressed genes. Compared with the TA_0 group, 59 pathways were significantly altered, and the pathways of “starch and sucrose metabolism,” “retinol metabolism,” “arachidonic acid metabolism,” “lysosome,” and “amino sugar and nucleotide sugar metabolism” were highly enriched in the TS_0 group. Compared with the TS_0 group, six pathways were significantly altered, and the pathways of “dilated cardiomyopathy,” “complement and coagulation cascades,” “cardiac muscle contraction,” “fructose and mannose metabolism,” “cGMP-PKG signaling pathway,” and “beta-alanine metabolism” were significantly enriched in the TS_800 group. Metabolomic analysis showed that a total of 107 differential metabolites (DMs) were identified in the TS_0 vs. TA_0 group, while 75 DMs were identified in the TS_800 vs. TS_0 group. Based on KEGG annotation, it was found that a large amount of DM was significantly enriched in amino acid metabolism pathways in the TS_0 group, including “arginine and proline metabolism,” “alanine, aspartic acid, and glutamic acid metabolism,” “β-Alanine metabolism and tyrosine metabolism” indicated that tannins affect the metabolism of amino acids. The integration of DEGs and DMs indicates that dietary tannins highly alter the digestion and absorption functions of proteins, as well as the biosynthesis and metabolism of amino acids. This study provides new insights into the adaptation of Pacific white shrimp to ammonia stress and the addition of tannins to feed to enhance immune function.