Modification Of Function Research Articles

Vitrification affects the post-implantation development of mouse embryos by inducing DNA damage and epigenetic modifications

Vitrification is widely used in assisted reproductive technology (ART) for female infertility, but the long-term effect on the embryo of vitrification has not been comprehensively studied. The study aimed to investigate the effect of vitrification on long-term development of mouse embryos. The warmed embryos which were frozen at 8-cell stage were cultured in vitro until the blastocyst stage and were transferred into recipients. Immunofluorescence staining was performed to evaluate the reactive oxygen species (ROS) level, mitochondrial function, cell apoptosis, DNA damage and histone epigenetic modification in blastocysts. Transmission electron microscopy (TEM) analysis was performed to exam the mitochondrial ultrastructure in blastocysts. The related gene expression and transcriptome profiles were investigated by RT-qPCR and RNA-seq, respectively. Blastocyst and implantation frequencies were not significantly affected by vitrification. However, vitrification significantly reduced blastocyst cell number and the live pup frequency. Vitrification induced ROS accumulation, DNA damage, and apoptosis in mouse blastocysts. The homologous recombination (NHEJ) is the major DNA repair pathway for vitrified embryos. Vitrification elevated H3K4me2/3, H4K12ac, and H4K16ac levels and reduced m6A modification in blastocysts. Moreover, vitrification significantly altered transcriptome profiles of mice placentas and brains at embryonic day 18.5 (E18.5). Thus, vitrification exhibited a long-term effect on mouse embryo viability by increasing ROS levels, DNA damage, altering the epigenetic modifications and transcriptome profiles.

Functionalized MXenes for Enhanced Visible-Light Photocatalysis: A Focus on Surface Termination Engineering and Composite Design

MXenes, a groundbreaking class of two-dimensional (2D) transition metal carbides, nitrides, and carbonitrides, have emerged as highly promising materials for photocatalytic applications due to their unique structural, electrical, and surface properties. These materials are synthesized by selectively etching the A layer from MAX phases, yielding compositions with the general formula Mn+1XnTx, where M is a transition metal, X represents carbon or nitrogen, and Tx refers to surface terminations such as –OH, –O, or –F. This review delves into the advanced synthesis techniques of MXenes, including fluoride-free etching and molten salt methods, and explores their potential in photocatalysis for environmental remediation. MXenes exhibit remarkable light absorption capabilities and efficient charge carrier separation, making them highly effective for the photocatalytic degradation of organic pollutants under visible light. Modulating their surface chemistry and bandgap via functional group modifications further enhances their photocatalytic performance. These attributes position MXenes as next-generation materials for sustainable photocatalytic applications, offering significant potential in addressing global environmental challenges.

Genetic and neurochemical profiles underlying cortical morphometric vulnerability to Parkinson's disease.

Increasing evidence has documented cortical involvement at all stages of PD. The local vulnerabilities within certain brain regions in PD have been previously demonstrated, whereas its underlying genetic and neurochemical factors remain unclear. This study aims to investigate the spatial spectrum of cortical atrophy in Parkinson's disease (PD) and link these variances in gray matter properties and curvature respectively to putative molecular pathways and neurotransmitter factors. We recruited 141 clinically diagnosed PD patients and 70 healthy controls. Cortical morphological abnormalities of PD were obtained by intergroup comparisons in gray matter properties metrics and curvature measurements. Then we performed gene-category enrichment and spatial correlation analyses to evaluate the specific correspondence between cortical alteration in PD and genetic expression from the Allen Human Brain Atlas and normative neurotransmitter atlases from Neuromaps. We found decreased gray matter properties in temporal, somatomotor, cingulate and occipital cortices, decreased curvature measures in occipital, temporal and orbitofrontal cortices, and increased curvature measures in somatomotor, prefrontal and posterior parietal cortices for PD patients. The related genes were enriched for the glucose metabolism, mitochondrial function, and post-translational histone modifications processes. In addition, the serotonin and norepinephrine transporter devoted more to gray matter properties alterations while the dopamine, gamma-aminobutyric acid receptors, and norepinephrine transporter were strong contributors of curvature abnormalities in PD. Collectively, the present study offered interpretation of cortical morphological alterations and the cortical pathogenic theory in PD from genetic and neurochemical perspectives, which inspire further research on new pharmacotherapeutic approaches.

Efficient Iron-Catalyzed Site/Regioselective Phosphinoylation of Carbohydrates.

The selective incorporation of phosphorus groups into sugar molecules holds substantial synthetic and biological significance, yet this area of research remains largely unexplored. In this study, we successfully devised a novel method employing an efficient Fe(III) catalyst for achieving site/regioselective phosphorylation/phosphinoylation of carbohydrates. This methodology boasts several merits, including mild reaction conditions, the employment of cost-effective and readily available catalysts, and exceptional selectivity. This approach provides new perspectives and valuable tools for the functional modification of sugar molecules.

Modeling and adaptive NN control for SMA flexible actuating systems with modified GPI hysteresis model

In this study, a shape memory alloy (SMA) actuating system is constructed to achieve flexible actuation ability. To improve the actuating performance, an effective control scheme needs to be developed to address the negative effects caused by the nonlinear nature of the internal SMA material and accommodate complex operating conditions. A modified generalised Prandtl-Ishlinskii (MGPI) hysteresis model is proposed to accurately characterise the strong saturated asymmetric hysteresis nonlinearity in the SMA wires. The modification of the input shape function allows for greater flexibility in controller design. Using this hysteresis modelling approach, an adaptive neural network (NN) control with a command filter is designed to achieve superior control performance. Experimental results validate the effectiveness of the proposed controller strategy.

Design and Synthesis of Tetrahydropyrrolo[3,4-c]pyrazole Sigma-1 Receptor Ligands.

This study presents a series of tetrahydropyrrolo[3,4-c]pyrazole-based compounds designed as sigma-1 receptor (S1R) ligands, focusing on optimizing affinity and reducing off-target effects. We synthesized various derivatives from commercially available precursors and, through radioligand binding assays, assessed their binding affinity for S1R and sigma-2 receptor (S2R). Compound 19 (AD417), containing a benzyl group and an amide substituent, demonstrated notable S1R affinity (Ki = 75 nM) with 6-fold selectivity over S2R. Modifications on the pyrrolidine nitrogen were crucial in enhancing receptor interaction, as the protonated nitrogen likely interacts with Glu172 within the S1R binding site. Furthermore, to address hERG potassium ion channel inhibition, a known limitation in S1R drug development, we evaluated compound 19's cardiotoxicity potential. With an experimental hERG IC50 of 5.8 µM, significantly higher than verapamil's IC50 of 0.41 µM, and haloperidol's IC50 of 0.16 µM, compound 19 showed a safer profile, suggesting a reduced risk of cardiotoxicity. These findings underscore the role of nitrogen accessibility, structural flexibility, and functional group modifications in optimizing S1R ligand interactions and provide a promising foundation for developing safer S1R-targeted therapeutics with minimized hERG-related risks.

Epigenetic Mechanisms Driving Human Evolutionary Changes

A crucial aspect of our comprehension of human evolution is epigenetics, the study of heritable modifications in gene function that do not include modifications in the DNA sequence. This intriguing field of study emphasises on how reversible and environmental-sensitive mechanisms, such as histone, DNA methylation, and non-coding RNA activity, control gene expression and support phenotypic variation. These techniques allow animals to adapt to changing environments without experiencing permanent genetic changes, providing a flexible basis for evolutionary processes. Understanding human evolution now heavily relies on epigenetics. Since animals can adapt to changing environments without enduring permanent genetic changes, evolutionary processes have a flexible basis. Epigenetic modifications have closely linked characteristics such as immune system function and brain development throughout human evolution. Epigenetic patterns have been impacted by environmental factors like diet, illness, and climate, which has allowed organisms to flourish and procreate in a variety of ecological niches. With an emphasis on their function in human evolution, this study explores the molecular mechanisms underlying epigenetic control and how epigenetics allows humans to display amazing phenotypic plasticity. The ramifications of epigenetic studies in anthropology are also examined, with a focus on how modern humans differ from their hominid ancestors and how epigenetics influences cultural and social practices. This review further highlights the revolutionary potential of epigenetics in deciphering the intricacies of human evolution by fusing knowledge from genetics, anthropology, and environmental studies.

Cu-Ag/SBA-15 nano catalysts for the control of microorganisms in water

Because of their uniform and regular channels, adjustable pore size, large surface area, controllable wall composition, high hydrothermal stability, ease of functional modification, and good accessibility of larger reactant molecules, mesoporous siliceous SBA-15 is of excellent catalyst carrier that is highly versatile and has been used extensively to prepare a variety of supported catalysts with ideal catalytic properties. In this study, we report the synthesis, characterization, and catalytic application of Cu-Ag/ SBA-15 nanoalloy catalysts towards the control of microorganisms in drinking water has been reported. The Cu-Ag/SBA-15 nanoalloy catalysts with different molar mass ratio of copper to silver (Cu:Ag = 1: 0, 0.75: 0.25, 0.5: 0.5, 0.25: 0.75, 0: 1) keeping 1weight % total loading of copper and silver metals on SBA-15 support have been prepared by incipient wetness impregnation method and characterized by various characterization techniques like, low angle XRD, wide angle XRD, N2-physcisorption and scanning electron microscopy techniques. The anti-bacterial activity of the catalysts was measured qualitatively by testing the presence of coliforms in water after contacting with the catalyst at room temperature. These nanoalloy catalysts found to be effective in controlling the microorganisms in drinking water. Among the series of the catalysts prepared, 0.25Cu-0.75Ag /SBA-15 catalyst showed superior catalytic activity. The high catalytic performance of the catalyst is due to its high surface area.

Bilingualism Is Associated with Significant Structural and Connectivity Alterations in the Thalamus in Adulthood.

Language is a sophisticated cognitive skill that relies on the coordinated activity of cerebral cortex. Acquiring a second language creates intricate modifications in brain connectivity. Although considerable studies have evaluated the impact of second language acquisition on brain networks in adulthood, the results regarding the ultimate form of adaptive plasticity remain inconsistent within the adult population. Furthermore, due to the assumption that subcortical regions are not significantly involved in language-related tasks, the thalamus has rarely been analyzed in relation to other language-relevant cortical regions. Given these limitations, we aimed to evaluate the functional connectivity and volume modifications of thalamic subfields using magnetic resonance imaging (MRI) modalities following the acquisition of a second language. Structural MRI and fMRI data from 51 participants were collected from the OpenNeuro database. The participants were divided into three groups: monolingual (ML), early bilingual (EB), and late bilingual (LB). The EB group consisted of individuals proficient in both English and Spanish, with exposure to these languages before the age of 10 years. The LB group consisted of individuals proficient in both English and Spanish, but with exposure to these languages after the age of 14 years. The ML group included participants proficient only in English. Our results revealed that the ML group exhibited increased functional connectivity in all thalamic subfields (anterior, intralaminar-medial, lateral, ventral, and pulvinar) compared with the EB and LB groups. In addition, a significantly decreased volume of the left suprageniculate nucleus was found in the bilingual groups compared with the ML group. This study provides valuable evidence suggesting that acquiring a second language may be protective against dementia, due to its high plasticity potential, which acts synergistically with cognitive functions to slow the degenerative process.

Construction and application of an inducible transcriptional regulatory tool from Medicago truncatula in Saccharomyces cerevisiae

Transcriptional regulation based on transcription factors is an effective regulatory method widely used in microbial cell factories. Currently, few naturally transcriptional regulatory elements have been discovered from Saccharomyces cerevisiae and applied. Moreover, the discovered elements cannot meet the demand for specific metabolic regulation of exogenous compounds due to the high background expression or narrow dynamic ranges. There are abundant transcriptional regulatory elements in plants. However, the sequences and functions of most elements have not been fully characterized and optimized. Particularly, the applications of these elements in microbial cell factories are still in the infancy stage. In this study, natural regulatory elements from Medicago truncatula were selected, including the transcription factors MtTASR2 and MtTASR3, along with their associated promoter ProHMGR1, for functional characterization and engineering modification. We constructed an inducible transcriptional regulation tool and applied it in the regulation of heterologous β-carotene synthesis in S. cerevisiae, which increased the β-carotene production by 7.31 folds compared with the original strain. This study demonstrates that plant-derived transcriptional regulatory elements can be used to regulate the expression of multiple genes in S. cerevisiae, providing new strategies and ideas for the specific regulation and application of these elements in microbial cell factories.

Stereoselective Synthesis and Biological Evaluation of Perhydroquinoxaline-Based κ Receptor Agonists

The hydroxylated perhydroquinoxaline 14 was designed by conformational restriction of the prototypical κ receptor agonist U-50,488 and the introduction of an additional polar group. The synthesis of 14 comprised ten reaction steps starting from diethyl 3-hydroxyglutarate (4). The first key step was the diastereoselective establishment of the tetrasubstituted cyclohexane 7 by the reaction of dialdehyde 6 with benzylamine and nitromethane. The piperazine ring was annulated by the reaction of silyloxy-substituted cyclohexanetriamine 8 with dimethyl oxalate. The pharmacophoric structural elements characteristic for κ receptor agonists were finally introduced by functional group modifications. The structure including the relative configuration of the tetrasubstituted cyclohexane derivative (2r,5s)-7a and the perhydroquinoxaline 9 was determined unequivocally by X-ray crystal structure analysis. The hydroxylated perhydroquinoxaline 14 showed moderate κ receptor affinity (Ki = 599 nM) and high selectivity over μ, δ, σ1, and σ2 receptors. An ionic interaction between the protonated pyrrolidine of 14 and D138 of κ receptor anchors 14 in the κ receptor binding pocket.

Functional analysis and modification of anti-lipopolysaccharide factor (ALF) from the freshwater crab Sinopotamon henanense and preparation of a novel ShALF6-2 K-AgNPs complex.

Overuse of antibiotics has led to the emergence of drug-resistant bacteria and environmental problems. Antimicrobial peptides (AMPs) and silver nanoparticles (AgNPs) can potentially replace antibiotics. Therefore, it is possible to create composite nanostructures with synergistic bactericidal properties by combining AgNPs and AMPs. In this study, a novel anti-lipopolysaccharide factor 6, named ShALF6, was identified in the freshwater crab Sinopotamon henanense. Full-length ShALF6 is 654 bp long and contains a typical lipopolysaccharide-binding domain spanning from Cys51 to Lys72. ShALF6 is highly expressed in hemocytes and responds to infection by the gram-negative bacterium Aeromonas hydrophila. ShALF6 inhibited the growth of gram-negative bacteria by binding to them and disrupting their cell membranes. To alter the charge of ShALF6, the negatively charged glutamic acid (E) in the sequence was replaced with a positively charged lysine (K) and the modified protein was named ShALF6-2 K. The bacteriostatic activity of ShALF6-2 K was significantly enhanced by an increase in the protein's cations. ShALF6-2 K showed high binding efficiency after 36 h of co-incubation with AgNPs and modifying the surface potential of the AgNPs. ShALF6-2 K-AgNPs exhibited synergistic inhibition with enhanced effectiveness against gram-negative bacteria. Finally, the cytotoxicity of ShALF6-2 K-AgNPs was investigated. The combination of ShALF6-2 K and AgNPs significantly reduced the toxic effects of AgNPs on the cells. This study provides theoretical and experimental bases for the development of novel bioactive AMP-coated composite AgNPs.

Low-Dose Ionizing Radiation and Thyroid Diseases and Functional Modifications in Exposed Workers: A Systematic Review.

Background/Objectives: With technological development, ionizing radiation has found applications in numerous occupations. However, the determination and quantification of the damage resulting from exposure to it remains rather unclear, along with the damage to particular organs. The aim of this systematic review was to investigate the relationship between low-dose ionizing radiation (LDIR) in exposed workers and possible functional changes and cancer development in the thyroid gland. Methods: We included observational studies evidencing the correlation under study. Data extraction and analysis was conducted on all included studies. The research strategy included three electronic databases (PubMed, Scopus, and Web of Science). The systematic review followed PRISMA guidelines, and the research protocol was submitted to PROSPERO (CRD:42023425839). Results: The search initially yielded 166 articles and, once duplicates and irrelevant articles were removed, a total of 15 useful articles were reviewed. Qualitative analysis of the studies showed that the TSH value does not change following exposure, while a reduction in fT3 and an increase or reduction in fT4 can be observed. Furthermore, the correlation between thyroid cancer and occupational exposure to radiation was not shown with certainty, but there was some evidence of increased gland volume and nodule formation. Conclusions: Even at low doses, ionizing radiation adversely affects thyroid activity. In this regard, new studies should be carried out in order to further investigate and define this issue and, consequently, outline useful measures to ensure the protection of workers in contact with this particular physical agent.

A strategy for enhancing phosphine oxide passivation capacity of perovskite solar cells by fluorination

Perovskite solar cells have experienced rapid development in the last few years due to their excellent photovoltaic properties, and their efficiency and stability have attracted widespread attention. Passivating interfacial defects has been universally recognized as an effective performance enhancement strategy for perovskite solar cells (PSCs), but most reported strategies often fail to simultaneously meet the requirements of efficiency and stability. This paper proposes to enhance the passivation function of phosphine oxide by fluorination. On the one hand, P=O is used to form coordination bonds with Pb2+ in perovskite. On the other hand, the strong hydrophobicity of F gives perovskite excellent moisture stability and can hydrogen bond to organic cations in the perovskite. Thanks to its strong chelation with the defect sites, it achieved optimized energy level arrangement, suppressed non-radiative recombination, and excellent operation stability. Consequently, the efficiency of the optimized device increased by 21.6% with a remarkable enhancement of 40 mV in VOC and remained more than 90% of its initial efficiency after aging in air environment for 1000 h, improving both efficiency and stability. This study demonstrates a promising functional modification strategy for constructing efficient, stable, and environmentally friendly PSCs.

A PDMS/chitosan/MPMs composite film based on multi-field coupling enhancement for African swine fever virus P72 protein detection.

African swine fever (ASF) is an acute hemorrhagic disease in pigs caused by the African swine fever virus (ASFV), which has a high mortality rate and brought great damage to global pig farming industry. At present, there is no effective treatment or vaccine to combat ASFV infection, so early detection of ASFV has become particularly important. Therefore, the PDMS/chitosan/MPMs composite film was proposed to detect ASFV P72. PDMS mixed with chitosan particles were used to provide biological modification sites for the ASFV P72 antibody, which simplified the functional modification process. The specific binding of antigen and antibody leads to the mechanical deformation of the composite film, which was observedthrough optical method tochange the microsphere spacing. The polystyrene microsphere (PMs) spacing changes were acquired by simply measuring the diameters of the Debye ring diffracted by the PDMS/chitosan/PMs with a simple laser pointer. The conductive layer formed by AgNWs realize the transformation of biological signals to electrical signals. By conjugating PMs with Fe3O4 nanoparticles (MPMs) and applying a magnetic field, a magnetic-stress-electric coupling system has been constructed, which amplifies the deformation of the composite film and enhances the sensitivity of the flexible film. The experimental results showed that the flexible film has wide linear range of 10ng/mL-100μg/mL, and the optimal detection limit (LOD) is 0.059ng/mL, which was aboutone order of magnitude lower than that without magnetic field. In addition, the flexible film showed a good stability, specificity, and selectivity.

Comparative Structural and Biophysical Investigation of Lycosa erythrognatha Toxin I (LyeTx I) and Its Analog LyeTx I-b.

Background/Objectives: This study investigates the structural and biophysical properties of the wild-type antimicrobial peptide LyeTx I, isolated from the venom of the spider Lycosa erythrognatha, and its analog LyeTx I-b, designed to enhance antibacterial activity, selectivity, and membrane interactions by the acetylation and increased amphipathicty. Methods: To understand the mechanisms behind these enhanced properties, comparative analyses of the structural, topological, biophysical, and thermodynamic aspects of the interactions between each peptide and phospholipid bilayers were evaluated. Both peptides were isotopically labeled with 2H3-Ala and 15N-Leu to facilitate structural studies via NMR spectroscopy. Results: Circular dichroism and solid-state NMR analyses revealed that, while both peptides adopt α-helical conformations in membrane mimetic environments, LyeTx I-b exhibits a more amphipathic and extended helical structure, which correlates with its enhanced membrane interaction. The thermodynamic properties of the peptide-membrane interactions were quantitatively evaluated in the presence of phospholipid bilayers using ITC and DSC, highlighting a greater propensity of LyeTx I-b to disrupt lipid vesicles. Calcein release studies reveal that both peptides cause vesicle disruption, although DLS measurements and TEM imaging indicate distinct effects on phospholipid vesicle organization. While LyeTx I-b permeabilizes anionic membrane retaining the vesicle integrity, LyeTx I promotes significant vesicle agglutination. Furthermore, DSC and calcein release assays indicate that LyeTx I-b exhibits significantly lower cytotoxicity toward eukaryotic membranes compared to LyeTx I, suggesting greater selectivity for bacterial membranes. Conclusions: Our findings provide insights into the structural and functional modifications that enhance the antimicrobial and therapeutic potential of LyeTx I-b, offering valuable guidance for the design of novel peptides targeting resistant bacterial infections and cancer.

Identification of RNA Editing Sites Reveals Functional Modifications with the Addition of Methionine to the Daily Rations of Yaks.

Methionine is an amino acid necessary for the growth and development of all animals. Glutathione produced during methionine metabolism can reduce damage to cells caused by oxidative stress. Supplementing restricted amino acids in animals by scientific means will be beneficial to protein synthesis, which will affect the growth and development of animals and will bring huge economic benefits when applied to actual production and life. In this study, we collected three muscle tissues from 24 male Maiwa yaks, which were fattened for three months with different methionine concentrations in their diet. RNA-seq was performed to obtain expression reads. A total of 1116 editing sites were identified by at least two software; the editing site types were mainly T-to-C and A-to-G mutations. We found two significant RNA editing sites presenting high-risk editing types. One was located on the MSRA gene that regulates the reduction of methionine, and the other can make changes to the properties of encoded proteins. This provides further understanding of the mechanism of yak muscle tissue and regulation of gene expression after the addition of methionine to daily rations.

Quantitative assessment of left ventricular function by left ventricular pressure-strain loop in patients with end-stage renal disease.

A new and non-invasive technology of left ventricular pressure-strain loop (LV-PSL) has recently been used to provide information on myocardial work (MW) and identify subtle modifications in cardiac function. This study aimed to use LV-PSL for early identification of changes in LV structure and MW in patients with end-stage renal disease (ESRD). Methods： Seventy-two patients with ESRD were divided into two groups based on undergoing maintenance hemodialysis (MHD), namely the dialysis group (ESRD-D group) and non-dialysis group (ESRD-ND group). Thirty age- and sex-matched control participants were enrolled in the N group. Traditional echocardiography and LV-PSL measurements were conducted. The values of Global longitudinal strain (GLS), peak strain dispersion (PSD), global constructive work (GCW), global wasted work (GWW), global work index (GWI), and global work efficiency (GWE) were assessed. Results：The most prevalent anomaly in ESRD patients was left ventricular (LV) hypertrophy. The GLS value was significantly lower and PSD was higher in patients with ESRD than in controls. Furthermore, patients with ESRD had severely higher GWW values and lower GWE than the N group (p< 0.05). No significant differences were found in GWI and GCW between the three groups (p> 0.05). Correlation analysis showed that GCW, GWI and GWE were positively correlated with LV ejection fraction (EF) and negatively correlated with GLS. GWW was negatively correlated with LVEF and positively correlated with GLS and PSD. In addition, GWE was negatively correlated with PSD (all p< 0.05). Conclusions：Patients with ESRD have LV structural and functional abnormalities. LV-PSL measurement can be helpful in identifying these subclinical abnormalities. MHD did not change myocardial workload in patients with ESRD.

A Complex Intervention to Minimize Medication Error by Nurses in Intensive Care: A Case Study.

Background/Objectives: Medication errors are the most frequent and critical issues in healthcare settings, often leading to worsened clinical outcomes, increased treatment costs, extended hospital stays, and heightened mortality and morbidity rates. These errors are particularly prevalent in intensive care units (ICUs), where the complexity and critical nature of the care elevate the risks. Nurses play a pivotal role in preventing medication errors and require strategies and methods to enhance patient safety. This study aims to develop a comprehensive and evidence-based intervention to minimize medication errors by nurses in ICUs. Methods: This qualitative case study forms a part of a broader research project that commenced with a scoping review. Building on the review findings, a complex intervention was designed to address nurses' medication errors. A focus group of experts was conducted to validate the intervention designed, evaluating its contextual feasibility and relevance. Results: This study led to the development of a complex intervention whose relevance lies in its potential implementation within the studied context. The resulting intervention was structured around four main components-educational interventions, verification and safety methods, organizational and functional modifications, and an error reporting system-meticulously designed to leverage the ICU's existing resources. Conclusions: In conclusion, the proposed intervention has the potential to positively impact healthcare quality by reducing errors and promoting a culture of safety. Furthermore, this study's findings provide a relevant foundation for future research and practical applications, driving advancements in healthcare service excellence.

Using Zebrafish Models to Study Epitranscriptomic Regulation of CNS Functions.

Epitranscriptomic regulation of cell functionsinvolves multiple post-transcriptional chemical modifications of coding and non-coding RNA that are increasingly recognized in studying human brain disorders. Although rodent models are presently widely used in neuroepitranscriptomic research, the zebrafish (Danio rerio) has emerged as a useful and promising alternative model species. Mounting evidence supports the importance of RNA modifications in zebrafish CNS function, providing additional insights into epitranscriptomic mechanisms underlying a wide range of brain disorders. Here, we discuss recent data on the role of RNA modifications in CNS regulation, with a particular focus on zebrafish models, as well as evaluate current problems, challenges, and future directions of research in this field of molecular neurochemistry.