This paper proposes a multitask learning framework for probabilistic model updating by jointly using strain and acceleration measurements. This framework can enhance the structural damage assessment and response prediction of existing steel frame structures with quantified uncertainty. Multitask learning may be used to address multiple similar inference tasks simultaneously to achieve a more robust prediction performance by transferring useful knowledge from one task to another, even in situations of data scarcity. In the proposed model-updating procedure, a spatial frame is decomposed into multiple planar frames that are viewed as multiple tasks and jointly analyzed based on the hierarchical Bayesian model, leading to robust estimation results. The procedure uses a displacement–stress relationship in the modal space because it directly reflects the elemental stiffness and requires no prior knowledge concerning the mass, unlike most existing model-updating techniques. Validation of the proposed framework by using a full-scale vibration test on a one-story, one-bay by one-bay moment resisting steel frame, wherein structural damage to the column bases is simulated by loosening the anchor bolts, is presented. The experimental results suggest that the displacement–stress relationship has sufficient sensitivity toward localized damage, and the Bayesian multitask learning approach may result in the efficient use of measurements such that the uncertainty involved in model parameter estimation is reduced. The proposed framework facilitates more robust and informative model updating.