Steel fibre-reinforced concrete (SFRC) slabs are ideal for modular construction owing to the shorter span of slab in standardised modules. To investigate the structural behaviours of SFRC floor system designed for modular construction, nine full-scale slab specimens were tested both at ambient temperature and under fire conditions. The crack pattern, failure mode, concrete spalling phenomenon, deformation capacity, load-carrying capacity and fire resistance time of the SFRC slabs are reported and compared with those of conventional steel rebar-reinforced concrete slabs. The experimental results showed that SFRC slabs achieved sufficient load carrying-capacity and fire resistance time for the application in modular buildings. A fibre-element model is proposed for computing the temperature distribution and cross-sectional moment resistance of SFRC slabs under fire conditions. Based on the experimental findings, design recommendations are provided to determine the fire resistance of SFRC floor system in modular construction.