Full Resolution Research Articles

Reconstructing the Gravitational Hologram with Quantum Information

Maldacena’s 1997 discovery of gauge/gravity duality promised to provide a complete understanding of quantum effects in gravity, by relating gravitating quantum systems to conventional quantum field theories on fixed backgrounds. Although much has been learned, this initial promise has not been completely fulfilled, due to lingering questions associated with the basic dictionary describing the way observables are mapped between one description and the other. While the dictionary is under good control for observables near the boundary, and also at the level of perturbation theory about a classical bulk spacetime for observables further in, non-perturbative questions critical for a full resolution of the black hole information problem remain to be fully answered. Over the last few years, however, a number of developments arising from the application of quantum information theory to AdS/CFT seem to be indicating that it may now be possible to address some of these questions. This GGI workshop brought together experts and young researchers, in order to capitalize on these developments.

Abstract 2069: High content imaging without sacrificing coverage, resolution or speed: Functional validation through multiple assays

Abstract Traditionally, high content screening (HCS) required compromise: sacrificing resolution, sample coverage or speed. This has limited high content screening’s utility as a high throughput tool, as speed is essential to cancer research at scale. Using a new approach, Araceli Endeavor® offers a solution to this compromise. This system images 96, 384 or 1536 -well high content microplates in under 10 minutes with full well coverage and sub-micron resolution, enabling high content screening at high throughput speeds without compromising resolution or coverage. Paired with the Clairvoyance™ analysis software, plates are analyzed at similar speeds, going from images to object-level data in 5-25 minutes. As with any new technology, validation is needed. Here we validate Endeavor and Clairvoyance at the level of the bioassay, using a range of common high content assays across different modalities. Assays are chosen to quantifiably demonstrate sensitivity, breadth, and broad applicability. Performance was benchmarked by using well-characterized compounds, matching published EC50/IC50 values when possible. We examined submicron-level nuclear spot counting, measuring histone yH2Ax and micronuclei formation after genotoxic insult. Intensity-based assays include cellular positivity, here live/dead, and subcellular translocation with transcription factor NFΚB after induction with cytokines Il1b and TNFα. Looking at cytosolic vesicle aggregation, we measured autophagic flux with two distinct modalities, comparative imaging and analysis of live cell dye and immunohistochemistry-based detection yielded consistent EC50 values between methods. Overall, these assays yield consistent, robust results corresponding to literature values, showing the importance of full well coverage and resolution. We demonstrate how assays that formerly took hours can be effectively imaged and analyzed without sacrificing quality in 10-30 minutes. Citation Format: Matthew Boisvert, Josh Kieler, Dan DiSepio, Don Weldon. High content imaging without sacrificing coverage, resolution or speed: Functional validation through multiple assays [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 2069.

Development of an easy-to-set-up multiple heart-cutting achiral-chiral LC-LC method for the analysis of branched-chain amino acids in commercial tablets.

In this paper, the development and application of a multiple heart-cutting achiral-chiral LC-LC method (mLC-LC) for the analysis of dansylated (Dns) branched-chain amino acids in commercial tablets are described. In the first dimension, a Waters Xbridge RP C18 achiral column was used under gradient conditions with buffered aqueous solution and acetonitrile. The elution order Dns-valine (Dns-Val)<Dns-isoleucine (Dns-Ile)<Dns-leucine (Dns-Leu) turned out with full resolution between adjacent peaks: 7.25 and 1.50 for the Val/Ile and the Ile/Leu pairs, respectively. A "research" validation study was performed, revealing high accuracy (Recovery%) and precision (RSD%) using two external set solutions, respectively, in the range 93.7%-104.1% and 0.4%-3.2%. The C18 column was connected via a two-position six-port switching valve to the quinidine-based Chiralpak quinidine-anion-exchange chiral column. A water/acetonitrile, 30/70 (v/v) with 50mM ammonium acetate (apparent pH of 5.5) eluent allowed getting the three enantiomers' pairs resolved: RS equal to 4.3 for Dns-Val and Dns-Ile, and 1.7 for Dns-Leu. The application of the mLC-LC method confirmed that the content of Val, Ile, and Leu in the tablets was compliant with that labeled by the producer. Only l-enantiomers were found in the food supplement, as confirmed by LC-MS/MS analysis.

Modeling and optimizing through plenoptic function for the dual lenticular lens-based directional autostereoscopic display system.

We propose an autostereoscopic display system that ensures full resolution for multiple users by directional backlight and eye tracking technology. The steerable beam formed by directional backlight can be regarded as the result of sparsely sampling the light field in space. Therefore, we intuitively propose an optimization algorithm based on the characterization for the state of the steerable beams, which is computed in matrix form using the plenoptic function. This optimization algorithm aims to optimize the exit pupil quality and ultimately enhancing the viewing experience of stereoscopic display. Numerical simulations are conducted and the improvement in exit pupil quality achieved by the optimization scheme is verified. Furthermore, a prototype of the stereoscopic display that employs dual-lenticular lens sheets for the directional backlight has been constructed using the optimized optical parameters. It provides 9 independent exit pupils at the optimal viewing distance of 400 mm, with an exit pupil resolution of 1/30. The field of view is ±16.7°, the viewing distance range is 380 mm to 440 mm. At the optimal viewing distance 400 mm, the average crosstalk of the system is 3%, and the dynamic brightness uniformity across the entire viewing plane reaches 85%. The brightness uniformity of the display at each exit pupil is higher than 88%.

Harmonized diffusion MRI data and white matter measures from the Adolescent Brain Cognitive Development Study

The Adolescent Brain Cognitive Development (ABCD) Study® has collected data from over 10,000 children across 21 sites, providing insights into adolescent brain development. However, site-specific scanner variability has made it challenging to use diffusion MRI (dMRI) data from this study. To address this, a dataset of harmonized and processed ABCD dMRI data (from release 3) has been created, comprising quality-controlled imaging data from 9,345 subjects, focusing exclusively on the baseline session, i.e., the first time point of the study. This resource required substantial computational time (approx. 50,000 CPU hours) for harmonization, whole-brain tractography, and white matter parcellation. The dataset includes harmonized dMRI data, 800 white matter clusters, 73 anatomically labeled white matter tracts in full and low resolution, and 804 different dMRI-derived measures per subject (72.3 TB total size). Accessible via the NIMH Data Archive, it offers a large-scale dMRI dataset for studying structural connectivity in child and adolescent neurodevelopment. Additionally, several post-harmonization experiments were conducted to demonstrate the success of the harmonization process on the ABCD dataset.

Rapid reaction optimization by robust and economical quantitative benchtop 19F NMR spectroscopy.

The instrumental analysis of reaction mixtures is usually the rate-determining step in the optimization of chemical processes. Traditionally, reactions are analyzed by gas chromatography, HPLC or quantitative NMR spectroscopy on high-field spectrometers. However, chromatographic methods require elaborate work-up and calibration protocols, and high-field NMR spectrometers are expensive to purchase and operate. This protocol describes an inexpensive and highly effective analysis method based on low-field benchtop NMR spectroscopy. Its key feature is the use of fluorine-labeled model substrates that, because of the wide chemical shift range and high sensitivity of 19F, enable separate, quantitative detection of product and by-product signals even on low-field, permanent magnet spectrometers. An external lock/shim device obviates the need for deuterated solvents, permitting the direct, noninvasive measurement of crude reaction mixtures with minimal workup. The low field-strength facilitates a homogeneous excitation over a wide chemical shift range, minimizing systematic integration errors. The addition of the optimal amount of the nonshifting relaxation agent tris(acetylacetonato) iron(III) minimizes relaxation delays at full resolution, reducing the analysis time to 32 s per sample. The correct choice of processing parameters is also crucial. A step-by-step guideline is provided, the influence of all parameters, including adjustments needed when using high-field spectrometers, is discussed and potential pitfalls are highlighted. The wide applicability of the analytical protocol for reaction optimization is illustrated by three examples: a Buchwald-Hartwig amination, a Suzuki coupling and a C-H arylation reaction.

Predictors of non-primary auditory and vestibular symptom persistence following surgical repair of superior canal dehiscence syndrome.

Patients with superior canal dehiscence syndrome (SCDS) can present with a plethora of auditory and/or vestibular symptoms associated with a bony defect of the superior semicircular canal. While surgical repair is a reasonable option for patients with significant localizing symptoms, the degree of clinical improvement will vary among patients and poses challenges in outcome prediction. This study aims to assess the relationship between preoperative and postoperative symptoms and identify predictors of symptom persistence following repair. Retrospective chart review. Tertiary neurotology single-institution care center. The primary outcome was to determine the proportion of resolved and persistent primary (most bothersome) and non-primary audiologic and vestibular symptoms following SCD repair. Secondary outcomes included comparison of patient, operative and radiologic characteristics between patients with resolved vs. persistent symptoms. Standardized patient questionnaires including 11 auditory and 8 vestibular symptoms were administered to patients at their preoperative and follow-up visits. Patient pre- vs. postoperative survey results, demographic and clinical characteristics, operative characteristics, audiometric data and cervical vestibular evoked myogenic potential (cVEMP) thresholds were compared via univariate χ2 and multivariate binary logistic regression analyses between those patients reporting full postoperative resolution of symptoms and persistence of one or more symptoms. Radiologic computed tomography (CT) measurements of superior canal dehiscence (SCD) defect size, location, and laterality were also compared between these two groups. Of 126 patients (132 ears) included in our study, 119 patients (90.2%) reported postoperative resolution (n = 82, 62.1%) or improvement (n = 37, 28.0%) of primary (most bothersome) symptoms, while 13 patients (9.8%) reported persistence of primary symptoms. The median (interquartile range) and range between surgery and questionnaire completion were 9 (4-28), 1-124 months, respectively. Analyzing all symptoms (primary and non-primary) 69 (52.3%) and 68 (51.1%) patients reported complete postoperative auditory and vestibular symptom resolution, respectively. The most likely persistent symptoms included imbalance (33/65/67, 50.8%), positional dizziness (7/20, 35.0%) and oscillopsia (44/15, 26.7%). Factors associated with persistent auditory symptoms included history of seizures (0% vs. 7.6%, p = 0.023), auditory chief complaint (50.0% vs. 70.5%), higher PTA (mean 19.6 vs. 25.1 dB, p = 0.043) and higher cervical vestibular evoked myogenic potential (cVEMP) thresholds at 1000 Hz (mean 66.5 vs. 71.4, p = 0.033). A migraine diagnosis (14.0% vs. 41.9% p < 0.010), bilateral radiologic SCD (17.5% vs. 38.1%, p = 0.034) and revision cases (0.0% vs. 14.0%, p = 0.002) were associated with persistent vestibular symptoms. Neither SCD defect size nor location were significantly associated with symptom persistence (P > 0.05). Surgical repair for SCDS offers meaningful reduction in the majority of auditory and vestibular symptoms. However, the persistence of certain, mostly non-primary, symptoms and the identification of potential associated factors including migraines, PTA thresholds, cVEMP threshold, bilateral SCD, and revision cases emphasize the importance of individualized patient counseling and management strategies.

The AO327 Drift Survey Catalog and Data Release of Pulsar Detections

The AO327 drift survey for radio pulsars and transients used the Arecibo telescope from 2010 until its collapse in 2020. AO327 collected ∼3100 hr of data at 327 MHz with a time resolution of 82 μs and a frequency resolution of 24 kHz. While the main motivation for such surveys is the discovery of new pulsars and new, even unforeseen, types of radio transients, they also serendipitously collect a wealth of data on known pulsars. We present an electronic catalog of data and data products of 206 pulsars whose periodic emission was detected by AO327 and are listed in the Australia Telescope National Facility catalog of all published pulsars. The AO327 data products include dedispersed time series at full time resolution, average (“folded”) pulse profiles, Gaussian pulse profile templates, and an absolute phase reference that allows phase aligning the AO327 pulse profiles in a physically meaningful manner with profiles from data taken with other instruments. We also provide machine-readable tables with uncalibrated flux measurements at 327 MHz and pulse widths at 50% and 10% of the pulse peak determined from the fitted Gaussian profile templates. The AO327 catalog data set can be used in applications like population analysis of radio pulsars, pulse profile evolution studies in time and frequency, cone and core emission of the pulsar beam, scintillation, pulse intensity distributions, and others. It also constitutes a ready-made resource for teaching signal-processing and pulsar astronomy techniques.

Equivalent-time-active-cavitation-imaging enables vascular-resolution blood-brain-barrier-opening-therapy planning

Objective. Linking cavitation and anatomy was found to be important for predictable outcomes in focused-ultrasound blood-brain-barrier-opening and requires high resolution cavitation mapping. However, cavitation mapping techniques for planning and monitoring of therapeutic procedures either (1) do not leverage the full resolution capabilities of ultrasound imaging or (2) place constraints on the length of the therapeutic pulse. This study aimed to develop a high-resolution technique that could resolve vascular anatomy in the cavitation map. Approach. Herein, we develop BandPass-sampled-equivalent-time-active-cavitation-imaging (BP-ETACI), derived from bandpass sampling and dual-frequency contrast imaging at 12.5 MHz to produce cavitation maps prior and during blood–brain barrier opening with long therapeutic bursts using a 1.5 MHz focused transducer in the brain of C57BL/6 mice. Main results. The BP-ETACI cavitation maps were found to correlate with the vascular anatomy in ultrasound localization microscopy vascular maps and in histological sections. Cavitation maps produced from non-blood-brain-barrier disrupting doses showed the same cavitation-bearing vasculature as maps produced over entire blood-brain-barrier opening procedures, allowing use for (1) monitoring focused-ultrasound blood-brain-barrier-opening (FUS-BBBO), but also for (2) therapy planning and target verification. Significance. BP-ETACI is versatile, created high resolution cavitation maps in the mouse brain and is easily translatable to existing FUS-BBBO experiments. As such, it provides a means to further study cavitation phenomena in FUS-BBBO.

Retrospective Case Series of Spinal Cord Neurapraxia in Male Adolescent Athletes: Can These Athletes Return-to-Play?

While spinal cord neurapraxia (SCN) is a documented pathology in athletes, guidance for neurosurgeons evaluating these patients is sparse. Therefore, in a cohort of adolescent athletes with SCN, we sought to (1) review their presentation and management and (2) describe outcomes and return-to-play (RTP). A single-center, retrospective case series was conducted to examine adolescent athletes diagnosed with SCN from 2017 to 2022. SCN was defined as an episode of bilateral upper and/or lower extremity weakness/numbness after an impact during sport. Collected variables included demographics, presentation, management, outcomes, and RTP. Six patients were included (mean age = 14.5 ± 2.1 years, 100.0% male). Three American football players sustained tackle injuries, 1 ice hockey and 1 basketball player fell and landed on their head/neck, and 1 weight lifter sustained an axial load of weights to his neck. Motor symptoms ranged from quadriplegia to partial weakness. Full symptom resolution was seen in 6/6 patients: in <1 day for 3 patients, 2-3 days for 2 patients, and 1-2 months for 1 patient. All 6/6 patients received computed tomography of head/cervical spine and MRI of cervical spine, half of whom received computed tomography and MRI of thoracic/lumbar spine. No patient had congenital stenosis or abnormal T2 signal on MRI. Specific RTP recommendations varied. All 6 patients/parents were contacted at a mean of 16.6 ± 2.6 months from the injury. All patients returned to play at a mean of 5.7 ± 3.3 months. All 6 patients returned to their previous sports. Across all patients, no recurrence or neurological sequelae was endorsed in the follow-up period. Six athletes with SCN with no imaging abnormalities and full symptom resolution were able to RTP to their previous sport without future consequence. Symptom duration may not be clinically useful in determining the feasibility of RTP. Follow-up studies are warranted in this patient cohort to standardize RTP recommendations.

INVESTIGATING THE EFFICACY OF SURGICAL AND NON-SURGICAL TREATMENT OPTIONS FOR MORTON'S NEUROMA BASED ON THEIR SIZE

Data was collected for patients referred to the orthopaedic department at Forth Valley Royal Hospital with metatarsalgia who subsequently received an ultrasound. Patients found to have a Morton's neuroma were divided into groups based on its size.A total of 90 patients received an ultrasound scan and neuroma was confirmed in 58 with an alternative diagnosis found in 32 patients and a total of 42 were included in the final analysis.All 14 patients with neuroma &lt; 6mm reported resolution of symptoms. 4 (28.5%) underwent surgical excision as first line, 1 (7%) received a single corticosteroid injection and 9 (64%) were treated with metatarsal bars.There were 27 patients with neuroma &gt; 6mm; 8 (29.6%) underwent surgical excision as first line treatment, 5 (18.5%) received metatarsal bars and 14 (51.9%) received injections. 7 (25.9%) patients reported resolution of symptoms after 1 injection, 1 (3.7%) patient required 2 injections and 1 (3.7%) patient required 3 injections to achieve resolution. 5 (18.5%) patients required surgical excision following ongoing symptoms despite non-surgical treatment. 9 (33.3%) reported resolution of symptoms following injection. 5 (18.5%) reported resolution of symptoms following use of metatarsal bars.A total of 71% of patients with a neuroma measuring &lt; 6mm reported full resolution of symptoms with non-surgical treatment. For patients with neuroma &gt;6mm, 64.3% had resolution of symptoms with injections alone and 18.5% required surgical excision despite injection.In conclusion, there is a benefit to offering non-surgical treatment as first line in patients with a neuroma regardless of size.

Resolution of Radiculopathy Following Indirect Versus Direct Decompression in Single Level Lumbar Fusion.

Retrospective analysis. To evaluate resolution of radiculopathy in one-level lumbar fusion with indirect or direct decompression techniques. Patients ≥18years of age with preoperative radiculopathy undergoing single-level lumbar fusion with up to 2-year follow-up were grouped by indirect and direct decompression. Direct decompression (DD) group included ALIF and LLIF with posterior DD procedure as well as all TLIF. Indirect decompression (ID) group included ALIF and LLIF without posterior DD procedure. Propensity score matching was used to control for intergroup differences in age. Intergroup outcomes were compared using means comparison tests. Logistic regressions were used to correlate decompression type with symptom resolution over time. Significance set at P < .05. 116 patients were included: 58 direct decompression (DD) (mean 53.9y, 67.2% female) and 58 indirect decompression (ID) (mean 54.6y, 61.4% female). DD patients experienced greater blood loss than ID. Additionally, DD patients were 4.7 times more likely than ID patients to experience full resolution of radiculopathy at 3months post-op. By 6 months, DD patients demonstrated larger reductions in VAS score. With regard to motor function, DD patients had improved motor score associated with the L5 dermatome at 6months relative to ID patients. Direct decompression was associated with greater resolution of radiculopathy in the near post-operative term, with no differences at long term follow-up when compared with indirect decompression. In particularly debilitated patients, these findings may influence surgeons to perform a direct decompression to achieve more rapid resolution of radiculopathy symptoms.

Dynamic Spatial Focus for Efficient Compressed Video Action Recognition

Recent years have witnessed a growing interest in compressed video action recognition due to the rapid growth of online videos. It remarkably reduces the storage by replacing raw videos with sparsely sampled RGB frames and other compressed motion cues (motion vectors and residuals). However, existing compressed video action recognition methods face two main issues: First, the inefficiency caused by the usage of coarse-level information under full resolution, and second, the disturbing due to the noisy dynamics in motion vectors. To address the two issues, this paper proposes a dynamic spatial focus method for efficient compressed video action recognition (CoViFocus). Specifically, we first use a light-weighted two-stream architecture to localize the task-relevant patches for both the RGB frames and motion vectors. Then the selected patch pair will be processed by a high-capacity two-stream deep model for the final prediction. Such a patch selection strategy crops out the irrelevant motion noise in motion vectors, as well as reduces the spatial redundancy of the inputs, leading to the high efficiency of our method in the compressed domain. Moreover, we found that the motion vectors can help our method to address the possibly happened <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">static-issue</i> , which means that the focus patches get stuck at some regions related to static objects rather than target actions, which further improves our method. Extensive results on both the HMDB-51 and UCF-101 datasets demonstrate the effectiveness and efficiency of our method in compressed video action recognition tasks.

Measuring the motion of the eardrum using Digital Image Correlation

The human eardrum is a translucent curved membrane and is in direct contact with the middle ear bones. Studying this complicated biomechanical system remains challenging. Digital image correlation (DIC) is an optical technique that allows measuring the shape and displacement of curved surfaces in 3D. In this work, we discuss the advantages and disadvantages of using DIC to study the eardrum at frequencies around 1 kHz. Special attention is given to the application of speckle patterns required to perform DIC on the translucent eardrum. Additionally, we detail an alternative approach to high-speed cameras: phase-locked light pulses. Using light pulses synced to the phase of the sound, regular DIC cameras can be used at their full resolution. This enables a high spatial resolution to thoroughly investigate the motion of the eardrum at the relevant frequencies.

Abstract TMP84: Multi-Institutional Validation of an Automated Segmentation Tool for Intracerebral Hemorrhage (ICH) and Peri-Hematomal Edema (PHE) on Head CT

Objective: In patients with acute intracerebral hemorrhage (ICH), the volume of hemorrhage, and perihematomal edema (PHE) are representative of primary, and secondary brain injury, respectively. Automated quantification of ICH and PHE volumes from admission non-contrast head CT can facilitate evaluation of large stroke datasets, and expedite treatment triage when volume cutoffs are applied. We aimed to train and externally validate an automated model for segmentation and quantification of ICH and PHE volumes on non-contrast head CT scans. Methods: For training of the model, we used the data from multicentric ATACH-2 clinical trial with head CTs from eleven medical institutes in six countries. The model was then exported and tested on two external datasets from Yale and University of Berlin. We designed an automated pipeline for extracting brain window from 3D non-contrast head CT, skull stripping, resampling to homogenous voxel size, and segmentation. For segmentation we applied a deep learning model based on 3D full resolution nnUNet. We used the Dice Similarity Coefficient (DSC), Hausdorff Distance (HD), and Volume Similarity (VS) between automated segmentation and ground truth manual segmentation volumes to evaluate the models’ performance. Results: A total of 854 patients from the ATACH-2 trial (854 х2, baseline and follow-up CT scans) were used for training of the model in 5-fold cross-validation. In validation folds, the model achieved a mean DSC=0.90±0.10, HD =1.42±16.0mm, and VS=0.95±0.10 for ICH; and DSC=0.74±0.12, HD =3.0±16.5 mm, and VS=0.91±0.12 for PHE. In the externaltesting cohort from Yale (200 patients х2, baseline and follow-up CT scans), the model archieved median DSC=0.93, HD=0.98 mm, and VS=0.97 for ICH; and median DSC=0.69, HD=4.52 mm, and VS=0.84 for PHE. In the externaltesting cohort from Charité Hospital, University of Berlin (915 baseline CT scans), the model archived median DSC=0.87, HD=4 mm, and VS=0.90 for ICH; and median DSC=0.65, HD=5 mm, VS=0.85 for PHE. Conclusion: We trained, validated, and externally tested an end-to-end automated tool for segmentation of both ICH and PHE on non-contrast head CT. Such tool can facilitate treatment triage for trials when volume cutoffs are applied for enrollment.

Full-resolution image restoration for light field images via a spatial shift-variant degradation network.

The light field (LF) imaging systems face a trade-off between the spatial and angular resolution in a limited sensor resolution. Various networks have been proposed to enhance the spatial resolution of the sub-aperture image (SAI). However, the spatial shift-variant characteristics of the LF are not considered, and few efforts have been made to recover a full-resolution (FR) image. In this paper, we propose an FR image restoration method by embedding LF degradation kernels into the network. An explicit convolution model based on the scalar diffraction theory is first derived to calculate the system response and imaging matrix. Based on the analysis of LF image formation, we establish the mapping from an FR image to the SAI through the SAI kernel, which is a spatial shift-variant degradation (SSVD) kernel. Then, the SSVD kernels are embedded into the proposed network as prior knowledge. An SSVD convolution layer is specially designed to handle the view-wise degradation feature and speed up the training process. A refinement block is designed to preserve the entire image details. Moreover, our network is evaluated on extensive simulated and real-world LF images to demonstrate its superior performance compared with other methods. Experiments on a multi-focus scene further prove that our network is suitable for any in-focus or defocused conditions.

Concentrated solar power for a reliable expansion of energy systems with high renewable penetration considering seasonal balance

With the increasing proportion of variable renewable energy characterized by fluctuation and the promotion of the “Clean Heating” policy, the problem of seasonal energy imbalance of the system has become increasingly challenging. There is a lack of effective means to mitigate this challenge under the background of gradual compression of the construction space for traditional thermal units. Concentrated solar power (CSP) is a promising technology to replace thermal units by integrating emergency boilers to cope with extreme weather, and can meet long-time energy balance as a seasonal peak regulation source. In this paper, we propose a long-term high-resolution expansion planning model of energy systems integrating CSP to address seasonal energy imbalances. The model takes into account both investment and operation considerations in full hourly resolution for the whole year based on a fast cluster optimization method. With the projection to 2050, we take the energy system in Xinjiang province which is a typical area of the “Clean Heating” project with rich irradiance as a case study. It shows that the optimal deployment of CSP and electric boiler (EB) can result in a 8.73 % reduction in costs, a 19.72 % decrease in the peak-valley difference of net load, and a substantial 58.24 % reduction in renewable curtailment at 65 % renewable penetration compared to the base scenario.

P1002 De novo and drug-induced skin manifestations in Inflammatory Bowel Disease patients: The impact of anti-tumour necrosis factor therapy

Abstract Background Skin disorders in inflammatory bowel disease (IBD) consist of extra-intestinal manifestations (EIM) or drug-induced lesions. This study explores de novo skin lesions and the emergence of immune-mediated skin disorders induced by anti-tumour necrosis factor (anti-TNF) agents. Methods Patients with EIMs, de novo skin lesions, and drug-induced skin eruptions were identified from an IBD patient database on advanced therapies. Data on time intervals from the initiation of IBD treatment to the development of skin disorders, causative agents, and subsequent management were collected. Results In a cohort of 1,773 patients (993 with ulcerative colitis, 746 with Crohn’s disease, and 31 with IBD unclassified), 30% (549) exhibited skin manifestations, with 28% (491 cases) showing de novo skin conditions. Identified skin manifestations included psoriasis/psoriasiform rash (18.3%), eczema (16.3%), seborrheic dermatosis (13.8%), acne (9.8%), actinic keratosis (5.3%), rosacea (4.7%), erythema nodosum (EN, 3.5%), cutaneous Crohn's disease (CCD, 2.6%), folliculitis (1.8%), pyoderma gangrenosum (PG, 1.5%), and others. Of the skin lesions, 31.8% (175) occurred before the IBD diagnosis, with a median duration of 8 years, while 51.9% (286) developed post-diagnosis unrelated to therapies. Biologic therapies improved extraintestinal skin lesions in 19 patients (3.4%). Notably, all 3 EN cases and 4 out of 5 PG cases achieved full resolution with biological treatments, leading to improved IBD activity. Similarly, 9 out of 10 Crohn’s disease patients with CCD showed improved skin conditions following biological treatments Fifty-eight patients (3.3%) experienced drug-induced skin lesions, with a median age at IBD diagnosis of 35 years (ranging from 17 to 67). The majority (91.3%) of cases were attributed to anti-TNF agents, and psoriasiform lesions were the most prevalent (60.3%). The median duration from treatment initiation to the onset of drug-induced skin lesions was 23 months (ranging from 0 to 115 months). Among the affected individuals, 19 using originator anti-TNF agents developed skin lesions, with 13 having psoriatic lesions. In comparison, 34 patients on biosimilar anti-TNF agents developed skin lesions, and 23 of them had psoriatic lesions. The progression of skin lesions led to anti-TNF discontinuation in 33 patients, of whom 25 transitioned to Vedolizumab or Ustekinumab. Resolution occurred in 22 patients. Conclusion Anti-TNF-induced skin lesions, notably psoriasiform lesions, appear to be more prevalent in the biosimilar era among IBD patients, often leading to discontinuation. Nevertheless, resolution is possible through strategic switches to alternative biologic agents.

Two-dimensional kinematics and dynamical modelling of the ‘Jackpot’ gravitational lens from deep MUSE observations

ABSTRACT We present results from the first spatially resolved kinematic and dynamical modelling analysis of the unique SDSSJ0946+1006 (‘Jackpot’) triple-source lens system, where a single massive foreground $z\, =\, 0.222$ galaxy multiple-images three background sources at different redshifts. Deep integral field unit spectroscopic data were obtained using the MUSE instrument on the VLT, which, compared to previous single-slit observations, provides full azimuthal area coverage, high sensitivity (5 h integration) and high angular resolution (0.5 arcsec full width at half-maximum). To account for the strong continuum contributions from the $z\, =\, 0.609$ source, a multiple-component stellar template fitting technique is adopted to fit to the spectra of both the lens galaxy and the bright lensed background arc simultaneously. Through this, we robustly measure the first and second moments of the 2D stellar kinematics out to about 10 kpc from the centre of the lens, as well as resolving the inner profile inwards to ∼1 kpc. The 2D kinematic maps show a steep velocity dispersion gradient and a clear rotational component. We constrain the characteristic properties of the stellar and dark matter (DM) mass components with a sufficiently flexible parametrised dynamical model and an imposed lensing mass and find a DM density slope of $\gamma \, =\, 1.73\substack{+0.17 \\ -0.26}$, i.e. significantly steeper than an unmodified NFW profile ($\gamma \, =\, 1$) and consistent with a contracted DM halo. Our fitted models have a lensing-equivalent density slope of $\eta \, =\, 0.96\pm 0.02$, and thus we confirm most pure lensing results in finding a near isothermal profile for this galaxy.

KIR2DS1 and KIR2DL1-C245 Dominantly Repress NK Cell Degranulation Triggered by Monoclonal or Bispecific Antibodies, whereas Education by Uptuning Inhibitory Killer Ig-related Receptors Exerts No Advantage in Ab-dependent Cellular Cytotoxicity.

NK cell responsiveness to target cells is tuned by interactions between inhibitory NK cell receptors and their cognate HLA class I ligands in a process termed "NK cell education." Previous studies addressing the role for NK cell education in Ab-dependent cellular cytotoxicity (ADCC) show ambiguous results and do not encompass full educational resolution. In this study, we systematically characterized human NK cell CD16-triggered degranulation toward defined human tumor cell lines in the presence of either the mAb rituximab or a recently developed CD34xCD16 bispecific killer engager. Despite positive correlation between killer Ig-related receptor (KIR)-mediated education and CD16 expression, NK cells educated by one or even two inhibitory KIRs did not perform better in terms of ADCC than uneducated NK cells in either missing-self or KIR-ligand matched settings at saturating Ab concentrations. Instead, NKG2A+ NK cells consistently showed more potent ADCC in the missing-self context despite lower levels of CD16 expression. KIR2DS1+ NK cells demonstrated dampened ADCC in both the missing-self and KIR-ligand matched settings, even in the presence of its ligand HLA C2. The lower response by KIR2DS1+ NK cells was also observed when stimulated with a bispecific killer engager. Surprisingly, repression of ADCC was also observed by NKG2A+ NK cells coexpressing the inhibitory KIR2DL1-C245 receptor that confers weak education. In conclusion, our study suggests that NK cell education by inhibitory KIRs does not augment ADCC per se, whereas expression of KIR2DS1 and KIR2DL1-C245 dominantly represses ADCC. These insights add to the fundamental understanding of NK cells and may have implications for their therapeutic use.