Soccer players are divided into six field positions, and players in side positions (fullbacks, wide midfielders) often demonstrate distinct kicking leg preference in actions such as crossing and long distance passing. These preferences can lead to strength and morphological asymmetries (MA) which can result in large changes in myodynamic characteristics of the muscles, particularly in the preferred (kicking) leg. PURPOSE: To compare anthropometric parameters and body composition (BC), and to identify MAs in relation to field position (FP) in elite soccer players. METHODS: The monitored players (n = 104, age 18.2 ± 0.6 yrs) of the highest Czech league (U19) were divided according to FP into: goalkeepers (GK), fullbacks (FB), central defenders (CD), wide midfielders (WM), central midfielders (CM), and attackers (AT), and were tested using Bioelectrical Impedance Analysis. The following parameters were observed: Body Height (BH), Body Mass (BM), Lean Body Mass (LBM), relative Lean Body Mass (LBMr), percentage of Fat Mass (FM), the ratio Extra to Body Cell Mass (ECM/BCM), as well as segmental proportion of muscle mass in upper extremities, trunk, lower extremities and percentage differences between the upper extremities (ΔUE) and lower extremities (ΔLE). RESULTS: Multilevel analysis of variance revealed significant differences in means of the selected variables between the FPs (F(65.410) = 2.06, λ = .26, p < .01, η2 = .23). The criterion of “field position” had a significant effect on all screened variables apart from ECM/BCM, FM, LBMr, and ΔUE (p > .05). FBs and WMs are significantly smaller and lighter in comparison to other FPs (p < .01). We detected significant differences in ΔLE with respect to FPs (F(5.98) = 6.53, p < .01, η2 = .25). Significant differences were also found in FBs (3.54 ± 1.20 %) and WMs (3.58 ± 1.39 %) in comparison to CDs (2.19 ± 1.11 %), ATs (2.04 ± 1.62 %) and GKs (1.14 ± 0.96 %). CONCLUSION: Research has shown differences between anthropometric indicators and body composition parameters in players on the basis of division according to their FPs. Maladaptive effects in terms of different segmental muscle mass proportion between the preferred and non-preferred leg may represent a potential risk of a player’s injury, especially for FBs and WMs. Supported by PRVOUK P38.