Power production, as well as transportation, are two sectors mainly contributing to global warming and pollution. These effects can be lessened through the use of biofuels. However, biofuels are plagued due to low calorific value and high viscosity compared to diesel. However, this can be overcome by proper adjustment of the operating parameters (Compression ratio, Fuel injection timing, Fuel injection Pressure, Engine load). The present study tries to unravel the influence of fuel injection pressure on the performance, combustion, emission, and exergy analysis of the Nahar biodiesel run diesel engine. Five fuel injection pressure (200 bar, 220 bar, 240 bar, 260 bar, 280 bar) and five engine loading conditions (20 %, 40 %, 60 %, 80 %, 100 %) at injection timing of 23° BTDC and compression ratio of 17.5 are considered. The results indicate that the performance, combustion, emission, and exergy parameters improve with the use of higher fuel injection pressure. The maximum BTEs are found to be 24.86 %, 27 %, 28.11 %, 29.22 %, and 31.13 % in biodiesel mode at FIPs of 200 bar, 220 bar, 240 bar, 260 bar, and 280 bar, respectively in comparison to 27.76 % for diesel at standard settings (FIP = 200 bar, CR = 17.5, IT = 23° BTDC) at 100 % load. Furthermore, the effect of addition of nanoparticles and variation of other operating parameters such as injection timing and compression ratio of the engine may enhance the performance and emissions of Nahar biodiesel run diesel engine.