pH-activated liquid microdroplet carrier for enhanced phycocyanin oral delivery and sustained bioactivity post-digestion.

Cold plasma induced amyloid β-lactoglobulin fibrils-fucoidan self-assembled complexes for lycopene delivery: Structural characterization, emulsion stability, and in vitro release behaviour.

Colorectal cancer (CRC) remains a major therapeutic challenge due to systemic toxicity and poor tumor selectivity of conventional treatments. Thymoquinone (TQ), a natural anticancer compound, faces limited clinical utility because of poor solubility and bioavailability. To overcome these challenges, a fucoidan (FC)-coated methoxy poly(ethylene glycol)-poly(lactic acid) (mPEG-PLA) nanoparticle system was developed for targeted TQ delivery. The optimized FC-coated TQ nanoparticles (∼105 nm) exhibited high encapsulation efficiency (82.3 ± 0.77%) and low polydispersity (<0.2), enabling passive tumor targeting via the enhanced permeability and retention (EPR) effect. The formulation showed sustained, pH-responsive release and enhanced cytotoxicity in HCT-116 cells (IC50 = 68.97 ± 1.10 µM) compared to uncoated NPs and free TQ. Confocal microscopy confirmed efficient uptake, while Western blot analysis demonstrated a concentration-dependent increase in cleaved caspase-3 in HCT-116 and HT-29 cells, indicating apoptosis induction. In vivo, FC-coated TQ-NPs induced significant tumor regression (75.26 ± 2.24%) and prolonged median survival (49 days) in C26 tumor-bearing mice versus free TQ (22 days). Biochemical analysis showed normal hepatic (ALT, AST) and renal (BUN, creatinine) profiles, and cardiac histology remained intact, confirming biosafety. Thus, FC-coated mPEG-PLA-TQ nanoparticles enhance efficacy and safety, offering a promising nanoplatform for targeted CRC therapy.

Brown algal fucoidan (FUC) has attracted significant interest as an active ingredient in various medical applications. Advancing analytical methods for FUC is essential to support the broader utilization of brown algal resources. In this research we developed an environmentally friendly sensitive fluorescent probe for FUC determination by AuNPs-chitosan core-shell nanocomposites. The prepared core-shells were visualized as uniform monodispersed spherical particles with a dense core, transparent shell and a particle distribution of 8.33 ± 2.48 nm. The colloidal solution of the nanocomposites exhibited a localized surface plasmon resonance band at around 530 nm and a blue emission peak at 394 nm after excitation at 310 nm. Owing to FUC's abundant sulfate moiety, it could interact with the positive surface charge of the prepared core-shells leading to fluorescence quenching that correlated to FUC concentration (R2 = 0.991) over the range of 60-140 μg/mL with a detection limit of 11.81 μg/mL. The accuracy and precision of the prepared nanocomposites were evaluated by % recovery (98%-102%) and %RSD (< 2), respectively. Additionally, the developed method was successfully applied for FUC determination in lab-prepared capsule formulation. The environmental impact of the proposed technique was assessed using complex MoGAPI and Eco-scale showing excellent method greenness.

Carbon-dots crosslinked collagen- fucoidan hydrogel with anti-bacterial and immunomodulatory activities promotes wound healing.

Natural polysaccharides mediated "low-molecular-bridging" network in fermented soybean protein gels: Interaction, microstructure, and rheological properties.

Paraquat (PQ) is extremely toxic to humans and leads to hepatic inflammation. Fucoidan (FCN) regulates the progression of oxidative damage and inflammation. However, whether FCN mitigates PQ-induced hepatic damage remains undiscovered. This study aimed to elucidate the protective effects of FCN against PQ-induced hepatic injury both in vivo and vitro. Mice in PQ group were injected with PQ, MIHA cells in PQ group were exposed to PQ for 24 h, for the establishment of hepatic injury models. Hepatic injuries were assayed by serum alanine aminotransferase (ALT)/aspartate aminotransferase (AST), liver histology, and myeloperoxidase (MPO) activity. Hepatic oxidative stress was assayed by malondialdehyde (MDA), superoxide dismutase (SOD), Heme oxygenase-1 (HO-1), quinone oxidoreductase-1 (NQO-1), kelch-like ECH-associated protein 1 (Keap-1), and nuclear factor erythroid-2-related factor 2 (Nrf2). Hepatic inflammation was assayed by nucleotide-binding domain and leucine-rich repeat containing protein 3 (NLRP3), Caspase-1, interferon (IL)-18, IL-6, IL-4, IL-10, tumor necrosis factor (TNF)-α, inducible isoform of Nitric Oxide Synthase (iNOS), and nuclear factor kappa-B (NF-κB) p-p65. In mice, pre-/post- treatment of FCN mitigated PQ-induced weight loss and higher liver/weight ratio, hepatic injury (increased serum ALT/AST concentrations, and MPO activity), oxidative stress (increased MDA content and Keap-1 protein level, impaired SOD activity, mRNA expressions of Nrf2, SOD1/2, HO-1 and NQO-1, protein expressions of Nrf2 and HO-1), hepatic inflammation (increased mRNA expressions of NLRP3, Caspase-1, IL-6, TNF-α, and IL-18, serum IL-6 and TNF-α contents, protein expression of p-p65), and hepatic mitochondrial dysfunction (increased mRNA level of iNOS). Meanwhile, in MIHA cells, FCN dose-dependently mitigated PQ-induced oxidative stress (increased protein expression of Keap-1, decreased mRNA expressions of Nrf2, SOD1/2, HO-1 and NQO-1, Nrf2 and HO-1 protein expressions), and inflammation (increased p-p65 protein expression, IL-6, TNF-α, and IL-18 mRNA expressions and decreased IL-10 and IL-4 mRNA expressions). FCN prevents PQ-induced hepatic injury by attenuating oxidative stress and inflammation via activating Nrf2/Keap-l signaling in vivo and in vitro.

Fucoidan reduces the occurrence of Benzo(a)pyrene-induced lung cancer by alleviating the abnormal changes of club stem cells.

Anthocyanin-loaded complexes of glycated dual milk-derived proteins: thermal stability, storage stability, and simulated digestion.

Fucoidan enhances stability of gelatin/anthocyanin-based oleogels via hydrogen-bonded crosslinking.

An anthocyanins-loaded extracellular vesicles coated with fucoidan nano-delivery system for enhancing dietary defense against inflammation-related colon injury.

Insights into the effects of fucoidan and caffeic acid on pasting, thermal, rheological, freeze-thaw and structural properties of cassava starch.

Construction of fucoxanthin-loaded multi-functional pea protein isolate-fucoidan nanoparticles at neutral pH: Structural characterization and functional verification.

Development and characterization of quinoa protein-fucoidan emulsion gels with excellent rheological properties for 3D printing and curcumin encapsulation stability.

Investigation on the mechanism of anionic polysaccharides affecting the gastrointestinal digestion fate of sea cucumber collagen fibrils.

The characterization of fucoidan‑sodium caseinate electrostatic complexes with application for pH-triggered release: Microstructure and digestive behavior.

Porcine epidemic diarrhea virus (PEDV) causes severe intestinal damage, posing significant threats to the swine industry. Fucoidan (FUC), a biologically active compound, exhibits antiviral activity against multiple viruses. This study aimed to investigate the protective effects of FUC on PEDV-induced intestinal injury in piglets and explore its underlying mechanisms. A total of 28 healthy crossbred piglets were randomly allocated into four experimental groups using a 2 × 2 factorial design: (1) a control group, (2) an FUC group, (3) a PEDV group, and (4) an FUC+PEDV group. From day 4 to day 10, the piglets in the FUC and FUC+PEDV groups were orally administered fucoidan at a dosage of 20 mg/kg body weight (BW) each day. On day 8, the piglets in the PEDV and FUC+PEDV groups were orally administered PEDV at a dose of 3 × 105.5 TCID50. The results show that FUC supplementation significantly decreased plasma DAO activity (p < 0.05) and increased the villus height, villus area, as well as the villus height/crypt depth (p < 0.05) in the intestine when compared to the PEDV-infected piglets. This indicates that FUC could alleviate the disruption of intestinal morphology and function caused by PEDV infection. FUC enhanced the antioxidant capacity of the piglets by increasing SOD and GSH-Px activity. Transcriptional profiling combined with quantitative analysis revealed that FUC regulates immune responses, substance transport, and arginine metabolism. Notably, FUC downregulated arginase 1 expression, which may redirect arginine toward nitric oxide synthesis, thereby establishing an antiviral state in the host. These findings highlight the potential application of FUC as a natural agent for mitigating PEDV-induced intestinal damage and improving gut health. Additionally, monitoring the health status of piglets is necessary when FUC is applied in practical applications.

Neural injury following ischemia-reperfusion (I/R) is induced by multiple pathophysiological pathways. This study aimed to use mitochondrial calcium channel and p-selectin inhibitors to weaken these pathways. One hundred and two rats were randomly divided into six groups. In the sham group, cerebral I/R induction and drug intervention were not performed. In the I/R group, cerebral I/R induction was induced. In the RR + FCN group, animals received only ruthenium red (RR) and fucoidan (FCN) intraperitoneally without I/R induction. In the I/R + RR group, animals received RR during the cerebral I/R period. In the I/R + FCN group, FCN was administered along with cerebral I/R. In the I/R +(RR + FCN) group, animals exposed to cerebral I/R received a combination of RR and FCN simultaneously. The shuttle box and new object tests were used to assess learning and memory. The superoxide dismutase (SOD), malondialdehyde (MDA), interleukin-1 beta (IL-1β), and tumor necrosis factor-alpha (TNF-α) levels in the hippocampus were measured. Neuronal death in the hippocampal CA1 area was assessed via hematoxylin-eosin staining. FCN and RR significantly decreased the tissue MDA, IL-1β, TNF-α levels while increased the SOD level. These inhibitors significantly reduced learning disorders and cerebral edema following I/R. The rate of neuronal death was significantly lower in each of the receiving RR and FCN groups. This study revealed that the use of FCN and RR significantly attenuated the pathways associated with oxidative stress and inflammation as well as neuronal death following cerebral I/R, thereby reducing learning and memory impairments. The effects of neuroprotection were further determined when two inhibitors were used simultaneously. HIGHLIGHTS: Cerebral ischemia-reperfusion is associated with many neurological, sensory and motor defects. Multiple pathways of neural pathophysiology are activated during cerebral ischemia-reperfusion. The Administration of ruthenium and fucoidan weakens inflammatory pathways, oxidative stress, and learning dysfunctions caused by cerebral ischemia and reperfusion. Stronger Neuroprotective effects were observed during the simultaneous administration of ruthenium and fucoidan.

Objective: This contemporary research reports the fabrication, optimization, and evaluation of Fucoidan (FDN)-loaded Nanosponges (NS) based gel for the treatment of rheumatoid arthritis. Methods: Risk assessment was performed, followed by screening and optimization of NS formulation by 32 factorial design using Design-Expert® software. FDN-loaded NS prepared by emulsion solvent evaporation technique was subjected to different solid and liquid state characterizations and subsequently loaded in carbopol gel. The effects of pro-inflammatory cytokines (IL-1 and TNFα) were evaluated using macrophage cells. Results: The physical and chemical characteristics exhibited by the prepared NS and gels (F1-F9) were found to be optimal. The optimization resulted in achieving formulation NS1 with 72.6% in vitro drug release and 8457cp viscosity and followed the Higuchi-matrix model. Histopathology studies revealed that prepared nanogel has promising anti-arthritic activity. The skin permeation studies showed that the optimized gel formulation was successful in stopping the drug from permeation through the skin. Moreover, the nanogel has depicted sustained drug release till 24 h. Conclusion: Hence, the NS-based the delivery system developed and assessed in the current research approach seemed to be auspicious concerning preventing rheumatoid arthritis along with practical utilization in the pharmaceutical field.

Antimicrobial peptide-fucoidan nanoplexes: A novel multifunctional biomimetic nanocarrier for enhanced vancomycin delivery against bacterial infections and sepsis.