FTIR Spectrophotometer Research Articles

AKTIVITAS TABIR SURYA FRAKSI METANOL DARI DAUN SIMPUR (Dillenia indica Linn.) DAN KARAKTERISASI SENYAWA ISOLATNYA

Sunscreen activity has been tested on simpur leaves (Dillenia indica Linn.) by determining the value of sun protection factor (SPF), erythema transmission percentage (%Te) and pigmentation transmission percentage (%Tp) of each methanol extract, n-hexane, dichloromethane fraction, and methanol fraction from simpur leaves. This test was carried out using a UV-Vis spectrophotometer (λ = 200-400 nm), a variation of sample concentration of 50; 75 and 100 ppm. The methanol fraction at a concentration of 100 ppm showed the highest SPF value of 4.630 ± 0.043 and the lowest %Te and %Tp values of 32.316 ± 0.297 and 52.699 ± 0.160, respectively. Isolation of flavonoids from the methanol fraction produced a relatively pure isolate (Rf = 0.901) with code G1 in the form of a yellowish-green solid/powder (1.5 mg). Characterization of isolate G1 using the FTIR spectrophotometer showed that there were vibrations of the alcohol O-H groups (3446.79 cm-1), aliphatic C-H (2958.80-2858.51 cm-1), C=C aromatic (1635.64 cm-1), C=C aromatic (1635.64 cm-1), C =O (1730.15 cm-1), C-O alcohol (1122.57 cm-1), and aliphatic C-H (1463.97-1382.96 cm-1). Based on a comparison of the spectra of isolate G1 with the spectra from the literature, isolate G1 was predicted as a flavonoid compound in the flavan or flavanol groups

Analisis Kadar Kurkumin pada Herbal Oil Kunyit Ekstrak Virgin Coconut Oil dengan Metode Ultrasonik dan Maserasi

Vegetable oil theoretically and experimentally has proven its potential as an alternative solvent in the extraction of natural materials. Turmeric infused with virgin coconut oil (VCO) is a way to take advantage of the active compounds from the herbs and the oil itself. This study aims to determine the ability of the VCO solvent in extracting curcumin compounds from turmeric with the addition of tween 80 surfactants. The extraction method used was maceration at 60℃ and an ultrasonic frequency of 40 kHz for 15 minutes. Thin layer chromatography (TLC) was used for qualitative analysis of each herbal oil extract using chloroform: methanol (95:5) eluent and FTIR spectrophotometer to identify functional groups. The highest curcumin content was found in herbal oil with tween 80 of 2 ml, namely 31 ppm in maceration extraction and 260.7 ppm in ultrasonic extraction. Identification of curcumin functional groups in herbal oils showed absorption patterns of O-H, C=O, aromatic C=C, C-O-C, and C-H.

Method validation of quantitative FTIR as a rapid and green analytical technique for dyes

Analysis and detection of dyes, whether in solutions or on substrates, are of the requirements in textile and coloration fields related to dyeing, printing, and coating, in addition to non-destructive testing. This research aimed to study the most effective chemical groups in dispersed dye red (C.I. 11338) structure. Different concentrations were prepared gravimetrically by calibrated and traceable weighing and volumetric tools. An accurate fixed volume of 25 micrograms of the studied solubilized dye was added quantitatively to the KBr disk as a fast technique in FTIR instead of using a liquid sample compartment. Linearity of the concentration with the peak intensity was used to validate FTIR spectrophotometer as a quantitative analysis method. Firstly, we detect the dye's most detectable function chemical groups and find the best linear correlation between the concentration and transmission intensities. The results revealed that the distinct chemical groups that can be used are: strong C=C bending at 748.28 cm-1, medium C=C bending at 808.07 cm-1, and O-H bending at 1411.71 cm-1, which give linear correlations of 0.9927, 0.9906 and 0.9998 respectively for concentration ranged from 0.001 to 0.05 ppm (w/v), LOD also studied and reached to a very low concentration less than 0.0005 ppm; the uncertainty of concentration at 1411.71 cm-1 was 0.02% with coverage factor 2 to give 95% confidence level. The result revealed the applicability of this technique in the quantitative analysis of dyes.

Characterization of M-curcumin complexes (M= Cu, Co, Ag) in turmeric rhizome as sensitizer candidates in dye-sensitized solar cell (DSSC)

This experimental research aims to characterize the complex compound M-curcumin (M=Cu, Co, Ag) based on turmeric (Curcuma longa Linn) rhizome as a candidate for photosensitizer in solar cells. The basic material used for manufacturing the M-curcumin complex compound in this study was curcumin from turmeric rhizome extract added to Cu2+, Co2+, and Ag+ metal ions. The results of the synthesis of the M-curcumin complex were characterized by FTIR and Uv-Vis spectrophotometers. The FTIR spectrophotometer test showed a curcumin compound in the viscous curcumin extract, characterized by an absorption wave number corresponding to the curcumin compound. In the M-curcumin complex, the bond between the metal and the ligand only appears in the Co-curcumin complex at the absorption number of 498.77 cm -1. The results of the UV-Vis spectrophotometer test showed that the maximum wavelength absorption in curcumin extract was 430 nm (abs = 0.688), Cu-Curcumin complex lmax 300 nm (abs = 2.573), Co-curcumin complex lmax 425 nm (abs = 1.067), and complex Ag-Curcumin lmax 430 nm (abs = 1.36). The UV-Vis and FTIR characterization showed that the Co-curcumin complex has good potential compared to the organic compounds (curcumin) and can be used as an alternative photosensitizer in solar cells

GREEN SYNTHESIS OF SILVER NANOPARTICLES FROM AQUEOUS LEAF EXTRACT OF MUSSAENDA ERYTHROPHYLLA AND ITS ANTIMICROBIAL ACTIVITY

The synthesis of silver nanoparticles from medicinal plant extracts has been carried out due to its environment-friendly approach. Mussaenda erythrophylla of the family Rubiaceae has traditional medicinal values. Silver nanoparticles are synthesized from the fresh leaf extract of this plant using AgNO3. The synthesis of nanoparticles is conrmed by the FTIR spectrophotometer which shows maximum absorption at 440 nm. The UV spectra and visual observation showed that the color of the fresh leaf extract turned brownish-yellow after forming silver nanoparticles. The antimicrobial activity of aqueous leaf extract and AgNPs on E.coli is studied by disc diffusion method. It is found that the AgNPs has more antimicrobial property than the leaf extract

Method validation of quantitative FTIR as rapid and green analytical technique for dyes

Analysis and detection of dyes, whether in solutions or on substrates, are of the requirements in textile and coloration fields related to dyeing, printing, and coating, in addition to non-destructive testing. This research aimed to study the most effective chemical groups in dispersed dye red (C.I. 11338) structure. Different concentrations were prepared gravimetrically by calibrated and traceable weighing and volumetric tools. An accurate fixed volume of 25 micrograms of the studied solubilized dye was added quantitatively to the KBr disk as a fast technique in FTIR instead of using a liquid sample compartment. Linearity of the concentration with the peak intensity was used to validate FTIR spectrophotometer as a quantitative analysis method. Firstly, we detect the dye's most detectable function chemical groups and find the best linear correlation between the concentration and transmission intensities. The results revealed that the distinct chemical groups that can be used are: strong C=C bending at 748.28 cm

Influence of succinic acid species on structural, spectroscopic,optical, Z-scan, frequency doubling, photoconductivity and antibacterial properties of glycine single crystals

With succinic acid as an additive, glycine single crystals were grown by conventional solvent slow evaporation route. Effect of succinic acid on the growth, optical and dielectric properties of glycine polymorphs has been investigated. The occurrence of functional groups has been identified by vibrational FTIR spectrophotometer. The low value of dielectric constant and dielectric loss at higher frequency range attested the grown crystal for frequency doubling applications. The laser damage threshold energy of the grown crystal was calculated. Third order nonlinear susceptibilityχ (3) (esu) of succinic acid added glycine crystals were evaluated from Z-scan experiment.

Characterization of Chemical Properties of Inulin Isolated from Yacón Tuber

Inulin is a polysaccharide with linear fructan chain structure. Food industries widely use inulin as a low calorie sugar substitutions. Yacón (Smallanthus sonchifolius) tuber that are easily grown and contain higher inulin can be explored as local inulin sources. This research aimed to characterize the chemical properties of inulin isolated from yacón tuber. The methods of this study include proximate analysis of yacón tuber, inulin isolation, and characterization of purified inulin using FTIR. The results showed that yacón consisted of 91.23 water (analyzed as moisture), 0.12 proteins, 0.58 fats, 0.52 crude fibers, and 7.34% carbohydrates. The yield of inulin extracted from yacón tuber was 4.86% whereas its purity and actual content of inulin were 44.23 and 2.15%, respectively. The Osazon test revealed that the isolated inulin has similar crystalline with that from chicory. Based on characterization using the FTIR spectrophotometer, the isolated inulin had functional groups of C-O, C-H, CH2, O-H, and C=O. The spectrum of the isolated inulin has similarities with chicory inulin. Therefore, yacón tuber could be considered as a potential local inulin source in Indonesia.

Utilization of Jengkol Peel (Pithecellobium jiringa) as an Adsorbent of Iron Metal

Cellulose, which has the active group OH, is found in Jengkol peel. Jengkol peel has the potential to be utilized as an adsorbent for Iron (Fe) metal because it contains these active groups. The FT-IR spectrophotometer instrument was used to confirm the presence of the -OH group in the Jengkol peel adsorbent, which was observed at the peak of the OH vibration at wave number 3293 cm1. Utilizing NaOH (0.1 M), the active group was activated. With an adsorption percentage of 96.24%, pH 7 was the optimum adsorption pH. With an adsorption percentage of 99.20%, 25 minutes was indeed the optimum contact time. With an adsorption capacity value of 8.581 mg/g, the binding of Fe metal by the adsorbent active group occurs in the complex formation mechanism, it was indicating a relatively high adsorption efficiency.

Bioautography and FTIR Analysis of Ethanol Fraction Morel Berry Root (Physalis angulata L.) Against Staphylococcus epidermidis and Pseudomonas aeruginosa

Morel berry root contains flavonoids, tannins, and alkaloids that have an antibacterial characteristics. This study aims to determine the antibacterial activity of the morel berry root ethanol fraction against Staphylococcus epidermidis and Pseudomonas aeruginosa and the most effective active compounds to inhibit such activity. Extractions of morel berry root used the maceration method with ethanol 70% of and then continued by fractionation using ethanol, chloroform, and n-hexane. Test of the compound fraction was performed using Thin Layer Chromatography (TLC) and characterization with FTIR Spectrophotometer. The results of the antibacterial activity with inhibitory zone diameter were at a 100% concentration of 18.69 mm in S. epidermidis while P. aeruginosa of 20.00 mm. Bioautographic results for ethanol fraction indicate an inhibited zone with an Rf value of 0.72 in the S. epidermidis and P. aeruginosa which is thought to be a flavonoid compound. Characterization with FTIR spectrophotometer functional groups O-H phenol, C = C aromatic, C-H aromatic, C-O alcohol, and C-H aliphatic in ethanol fraction with Rf 0.72.

Study the effect of kaolin on the electrical properties of polyvinyl acetate-polyol composite

Polyvinyl acetate and polyol (PVA-Polyol) has been blended in percentage by weight 3:1 and a mixture has been done by mixing different percentages by weight of kaolin for electrical measurements. Diagnosis of kaolin was measured by FTIR spectrophotometer. Quality factor (Q), dissipation factor (D), parallel resistance (R), pseries resistance (R), impedance (Z), series capacitance (C) and parallel capacitance ss(C) and Phase angle (Φ) were measured. The maximum electrical conductivity is pfound 6794 S.m-1 for samples 1, 3wt% kaolin and the minimum electrical conductivity is 1x10-5 S.m-1 for sample (2) 5wt% kaolin. Dielectric constants (έ), dielectric loss (έ′), have been studied and calculated. The frequency dependent ac conductivity (σ) and frequency independent dc conductivity (σ) has studied and acdcinvestigated. The calculated minimum and maximum ac conductivity (σ) is 3.2x10-5 acS.m-1 for samples (3) 6wt % kaolin and 0.45 S.m-1 for sample (2) 5wt % kaolin. The minimum and maximum calculated independent frequency dc conductivity (σ) is -dc 0.3472 S.m-1 for sample (2) 5wt % kaolin and 1.1671 S.m-1 for sample (1) 3wt % kaolin.

Spectrally selective single layered Ag@CuO nanocermet coatings for photothermal application: Green synthesis method

Herein, green synthesized single-layered Ag@CuO nanocermet coatings deposited using spin coating on stainless steel (SS) substrates at 700, 800, 900 and 1000 rpm as solar selective absorbers is reported. The solar absorbing coatings consist of plasmonic silver (Ag) nanoparticles (NPs) embedded in a semiconductor matrix of CuO. The morphological, structural, compositional, and thickness of the coatings were characterized using Scanning electronic microscopy (SEM), X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX), and Rutherford backscattering spectrometry (RBS). The SEM images of Ag@CuO nanocermet confirms the presence of better dispersibility of Ag NPs in the nanorod-like morphology of CuO. XRD patterns revealed well-crystalline nature of monoclinic CuO, and face centered cubic of Ag metal, and EDX spectra confirms the compositions of the coatings. RBS determined thicknesses of the coatings, and are found to be 1416 × 1015 atoms/cm2 (298.2 nm), 1296 × 1015 atoms/cm2 (272.8 nm), 1153 × 1015 atoms/cm2 (242.7 nm), and 998 × 1015 atoms/cm2 (210.2 nm) at 700, 800, 900, and 1000 rpm, respectively. The optical properties of the obtained coatings were analyzed using UV–Vis–NIR, and FTIR spectrophotometers in the wavelength range of 0.3–2.5 μm, and 2.5–20 μm, respectively. The Ag@CuO nanocerment coatings exhibit a solar absorptance (α = 0.93), and emissivity (ε = 0.23) at RS of 700 rpm. The embedded Ag NPs are responsible for enhancing the intrinsic absorption properties of CuO, due to the concentrated free electrons whose particle sizes are comparable to or smaller than the wavelength of incident light.

SYNTHESIS OF POLYELECTROLYTE COMPLEX FILMS OF CHITOSAN-ALGINATE BY ADDITION OF KELOR LEAVES EXTRACT (Moringa oleifera) FOR FOOD PACKAGING

This research aims to synthesize the polyelectrolyte complex (PEC) using chitosan and alginate as matrix film with the addition of kelor leaves extract (Moringa oleifera). The preparation of PEC films of chitosan-alginate by addition of kelor leaves extract (Moringa oleifera) had been implemented. The kelor leaves was prepared by drying process under the sun for removing water content and the dried kelor leaves was extracted using maceration technique. The final concentrations of film solution used in this study were 0.0% (Film E0), 0.50% (Film E1), 0.75% (Film E2), and 1.00% (Film E3). The PEC chitosan-alginate films with addition of kelor extract were prepared in room temperature (± 25 °C) and pH of film solution was ± 4.0. The result of film’s characterization using FTIR spectrophotometer showed that PEC films of chitosan-alginate were formed through the molecular interaction between chitosan which protonated into amine groups (–NH3+) and alginate which dissociated into carboxylate groups (–COO−). Based on FTIR spectrum, it also showed that there were some wavenumber shifts after addition of kelor leaves extract into PEC films of chitosan-alginate. It was confirmed that there were the changing of molecular interaction between PEC chitosan-alginate films due to presence of kelor extract, indicating that PEC chitosan-alginate films with kelor extract had been formed. Morphological analysis using a scanning electron microscope (SEM) displayed both porous and heterogeneous distribution on the surface after addition of kelor extract.

Synthesis and characterization of sulfonated poly(eugenol-co-allyleugenol) membranes for proton exchange membrane fuel cells

The research of sulfonated eugenol-allyleugenol copolymer (SPEAE) based membrane for fuel cell from eugenol derivate had been conducted. First, eugenol was reacted with various weights of allyl eugenol to form eugenol-allyleugenol copolymer (PEAE). Determination of the optimum composition of PEAE was done by testing the swelling properties. Then, PEAE was sulfonated using concentrated sulfuric acid with time variations of 1, 2, 3, 4, and 5 h to form SPEAE. The SPEAE produced was tested for the degree of sulfonation, water uptake, cation exchange capacity, and membrane proton conductivity. In addition, the characteristics of the PEAE and SPEAE copolymer membranes were also analyzed using FTIR spectrophotometers, 1H-NMR, TGA, and DSC. The results showed that the copolymerization of eugenol:allyleugenol (EG:AEG) with a ratio of 10:1 gave the lowest swelling degree. The best SPEAE copolymer was obtained from sulfonation for 2 h with yield, degree of sulfonation, water absorption value, proton conductivity, and cation exchange capacity of 90.6%, 12.87%, 50.7%, 1.83 × 10−5 S cm−1 and 0.356 meq/g, respectively. FTIR analysis shows the formation of PEAE with the loss of the vinyl eugenol groups used to form the polymer and shows the formation of SPEAE in the presence of sulfonate groups from the sulfonation reaction. 1H-NMR also confirmed the presence of the PEAE and SPEAE copolymers. In addition, analysis of thermal properties with TGA and DSC also showed that sulfonate treatment could improve membrane stability.

NiFe2O4 nanoparticles as nanozymes, a new colorimetric probe for 2,4-dichlorophenoxyacetic acid herbicide detection

• A Nano compound NiFe 2 O 4 synthesized. • The synthesized nanomaterial exhibit peroxidase-like activity. • New insight into the design of new nanozymes for cascaded catalytic detection and other low-cost, high-efficiency, and rapid detection applications. • New colorimetric method is described for the detection of 2,4-D herbicide residues. The use of enzyme mimics to detect herbicide residues quickly has recently sparked a lot of attention. The majority of nanozymes, nevertheless, are low active. Here, an active nanozyme (NiFe 2 O 4 ) that mimics peroxidase (POD-like) with improved activity was logically developed. The probe was systematically characterized by powder XRD, SEM, EDX, HR-TEM, UV–Vis absorption, FTIR and fluorescence spectrophotometers. Based on its high peroxidase-like activities and cascaded catalytic reactions with 2,4-dichlorophenoxyacetic acid (2,4-D), a new method is described for the detection of 2,4-D herbicide residues using 4-(Dimethylamino) benzaldehyde as chromogenic substrate in the presence of hydrogen peroxide. More importantly, the proposed method shows the limit of detection (LOD) of 0.311 µg.mL -1 and the recovery ranged from 97.74 to 101.44%. This study provided new insight into the design of new nanozymes for cascaded catalytic detection and other low-cost, high-efficiency, and rapid detection applications.

Chemical composition, antioxidant activity and cytotoxicity of flower extracts from Magnolia grandiflora L. found in southeast Mexico

Many reports state that different structures of M. grandiflora contain bioactive components. Nonetheless, phytochemical studies reported that the extracted essential oils are chemically different and remarkably variable in their qualitative and quantitative compositions. Further studies Mexican M. grandiflora needs to be done. Thus, the research aimed to characterize a) the chemical composition, b) the antioxidant activity and c) cytotoxicity effect of two M. grandiflora flower extracts. The chemical composition was evaluated by preliminary phytochemical test followed by thin layer chromatography, UV-Vis and FTIR spectrophotometer analysis. The antioxidant activity of the flower extracts was measured by the free radical-scavenging activity (ABTS) and the stable radical of 2,2-diphenyl-1-picryl-hydrazyl (DPPH) method and the cytotoxicity by an Artemia salina bioassay. Water and ethyl flower extracts showed the presence of organic chromophores such as flavonoids. Both extracts (ethyl and water) demonstrated antioxidant activity by both ABTS (459.6±8.5 µmol TE g-1 and 274.2±5.7 µmol TE g-1 respectively) and DPPH (3210.4 ± 2.5 µmol TE g-1 and 219.7 ± 0.9 µmol TE g-1 respectively) methologies; and non-cytotoxic activity (LC50 , µg mL-1) (1,285 ± 14 and 1,116 ± 15 respectively). The water and ethyl extracts of M. grandiflora flowers found in southeast Mexico are a promissory source of chemical compounds with attributed biological activity according to the presented results.

Bioadsorption of Fe (II) ions from aqueous solution using Sargassum latifolium aqueous extract and its synthesized silver nanoparticles

Algal extracts are used in the environmentally safe and economically advantageous biosynthesis of silver nanoparticles, which does not require the use of hazardous chemicals, high temperatures, pressures, or energies. In the current study, we created silver nanoparticles from the extract of the marine brown alga Sargassum latifolium, analyzed them with Transmission Electron Microscopy (TEM), FTIR, and UV-visible spectrophotometers, and used them to show how well they could remove Fe (II) ions from aqueous solutions. UV scan analyses of S. latifolium aqueous extract of silver nanoparticles showed a maximum peak at 450 nm. This peak is considered a characteristic peak for silver nanoparticles. Also, FTIR analysis of S. latifolium aqueous extract revealed various functional groups such as – OH, –NH, –CH, –COOH, CO, and C–C, which are responsible for bioadsorption of Fe (II). TEM also demonstrated that the synthesized nanoparticles were spherical, distinct, and regular, with particles size about 6.03–15.16 nm. S. latifolium aqueous extract silver nanoparticles were more effective than its aqueous extract in removing Fe (II) from an aqueous solution. The removal efficiency of Fe (II) by nanoparticles was 83%, while by the aqueous extract was 69%. The optimal conditions for bioadsorption of Fe (II) were pH 4, contact time 150, and adsorbent dose 0.01 g.

Investigation on synthesis of ternary g-C3N4/ZnO–W/M nanocomposites integrated heterojunction II as efficient photocatalyst for environmental applications

The rapid industrialization of the world is disparagingly manipulating our environment and natural ecosystem. The researchers are taking keen interest to invent novel material as photocatalyst for non-degradable organic pollutants. Solar energy-driven practices employing semiconductors are a novel approach towards wastewater remediation. Here in, we successfully synthesized a vigorous photocatalysts comprising of g-C3N4 and doped ZnO–W/M (M = Co, Ce, Yb, Sm) by co-precipitation followed by metals doping via calcination approach. The structural, morphological, and photocatalytic applications for organic pollutants of synthesized heterostructure nanocomposites were examined by XRD, FTIR, SEM, EDX and UV visible spectrophotometer. Diffraction peaks attributed to both g-C3N4 and ZnO–W were detected in the XRD spectra. The FTIR spectra also inveterate the formation of g-C3N4/ZnO–W/M composites. The SEM images reveal an agglomerated morphology and EDS analysis also confirmed close contact between g-C3N4, ZnO–W and doped metals. The abridged energy band gap of g-C3N4/ZnO–W/M (M = Ce, Yb, Sm, Co) nanocomposites calculated via Tauc plot are 2.68, 2.88, 3.24 and 3.29 eV respectively. Narrowing of bandgap is considered an imperative triumph for the degradation of industrial effluents. The photocatalytic activity was performed against four different dyes and follows the trend Ce > Yb > Sm > Co. The recyclability tests were carried out for different dyes and no substantial catalytic activity loss was observed even after the fourth experimental run, which proves that reported ternary heterojunctions exhibit high mechanical stability and reusability.The species trapping experiment exposed that generated h+ are the principal active specie for dye photodegradation reactions. This work disseminates a novel photocatalyst for the removal of synthetic dyes.

Synthesis of nanosilver with simpur leaf (Dillenia indica) modified PVA as antibacterial

Synthesis of silver nanoparticles using a bioreductant of simpur leaf extract to minimize the use of inorganic materials that are harmful and not environmentally friendly. Silver nanoparticles with the bioreduction method, the bioreductant solution was mixed with AgNO3 precursor and the addition of PVA concentrations was 0%, 0.75%, 1.5% and 3%. The purpose of this study was to determine the effect of PVA on the characteristics of silver nanoparticles on antibacterial activity. Analysis of functional groups in simpur leaf extract and Ag/PVA 0.75% using FTIR Spectrophotometer showed wave numbers 3443 cm-1 and 3441 cm-1 the presence of -OH groups and at wave numbers 1640 cm-1 and 1638 cm-1 the presence of C=O groups. UV-Vis analysis showed the optimum wavelength in the 3% PVA variation absorption region showed an absorbance value of 429 nm with an average size of 8.19 nm silver nanoparticles. Inhibition of silver nanoparticles against bacterial growth Escherichia coli and Staphylococcus aureus the highest concentration was at 100% with an inhibition zone diameter of 10.33 mm and 11.58 mm in the strong category.

Extraction of Phytochemicals from Maypole Apple by Subcritical Water.

The Maypole apple is a new, promising species of small apples with a prominent flavor and deep red flesh and peel. This study divided Maypole apples into outer flesh, inner flesh, and peel, and used subcritical water at 100-175 °C for 10-30 min to extract various phytochemicals (procyanidin B2 (PB2), 5-caffeoylquinic acid (5CQA), and epicatechin). The obtained Maypole apple extracts and extraction residues in this work were analyzed using a SEM, HPLC, FT-IR, and UV-Vis spectrophotometer. Under different subcritical water extraction conditions, this work found the highest extraction rate: to be PB2 from the peel (4.167 mg/mL), 5CQA (2.296 mg/mL) and epicatechin (1.044 mg/mL) from the inner flesh. In addition, this work regressed the quadratic equations of the specific yield through ANOVA and found that temperature is a more significant affecting factor than extraction time. This aspect of the study suggests that phytochemicals could be obtained from the Maypole apple using the new extraction method of subcritical water.