Fruit Research Articles

Draft genome sequences for Neonectria magnoliae and Neonectria punicea, canker pathogens of Liriodendron tulipifera and Acer saccharum in West Virginia.

The fungal genus Neonectria contains many phytopathogenic species currently impacting forests and fruit trees worldwide. Despite their importance, a majority of Neonectria spp. lack sufficient genomic resources to resolve suspected cryptic species. Here, we report draft genomes and assemblies for Neonectria magnoliae NRRL 64651 and Neonectria punicea NRRL 64653.

URBAN LANDSCAPE DESIGN - A COMMUNITY-ORIENTED PROPOSAL FOR CRAIOVA OLD TOWN

This study focuses on designing a green space in Craiova's Old Town, aiming to improve residents' well-being through nature integration. Using 3D landscaping software, a 270 m² area was designed with features like terraces covered in Wisteria sinensis, green walls, aromatic plants and fruit trees, promoting the concept of Edible City Solutions. The design also includes community-oriented elements such as a stage for events. This project serves as a model for sustainable urban green spaces, enhancing social interaction and environmental quality while fostering a deeper connection between people and nature

Effects of Different Tree Shapes on Canopy Characteristics of Zaosu Pear (Pyrus ssp. Va.) in the Northwest Arid Zone

Zaosu pear (Pyrus ssp. Va., Zaosu) is the main variety of pear cultivated in Gansu Province. Tree shape selection is important in pear cultivation: reasonable tree shapes affect the growth and development of fruit trees and fruit quality, which, in turn, affect the economic benefits of orchards. In this study, Zaosu pear was used as a test material, and five different tree shapes—Y, trunk, single-arm, double-arm, and spindle shapes—were studied regarding three aspects, namely, branching and leafing parameters, light radiation parameters, and root distribution, in order to provide a theoretical basis for the selection of pear tree shapes in arid areas. The results showed that the single-arm and double-arm shapes had the highest fruit weight per fruit values, reaching 280.06 g and 278.27 g, respectively, and the difference in soluble solids—12.42 mg·g−1 and 12.91 mg·g−1, respectively—was not significant; the mu yield of the single-arm shape was 3282 kg; and the yield of a single tree was 39.54 kg, which was the highest for the five tree shapes. The trees mainly had short fruiting branches when they entered the fruiting stage, and the double-arm shape had the highest number of short fruiting branches, which was significantly higher than those of the other tree shapes. Correlation analysis revealed that the number of long fruiting branches was significantly and positively correlated with axillary flower buds. The distribution of light intensity for all five tree shapes showed a gradual increase from bottom to top and from inside to outside, and the single-arm and double-arm shapes had a uniform distribution of light intensity, which translated into better growth and fruiting potential. The trunk shape had the largest leaf area index, resulting in too much depression within the canopy and reducing light, while the single-arm and double-arm shapes had the smallest leaf area indexes, resulting in the light being evenly distributed inside and outside the canopy, and the percentage of light intensity was greater than 50% in all cases, reaching up to 80%. The double-arm form had the highest chlorophyll a content of 24.55 mg·g−1, implying the ability to convert more light energy into chemical energy, and the content of chlorophyll a + b was highest in the single-arm form and the double-arm form, with values of 31.13 mg·g−1 and 33.65 mg·g−1. The total length of the root system and the total surface area of the root system for the single-arm shape were the largest, i.e., 558,550.64 cm and 79,252.32 cm2, respectively, indicating that it has stronger adaptability and resource acquisition ability compared to the other tree shapes. In conclusion, the single-arm form is more suitable for planting in arid areas.

A fruit tree feature matching algorithm based on an improved AKAZE method

A fruit tree feature matching algorithm based on an improved AKAZE method

First Report on the Bark Feeding Wasp Species on Lowland Apple and Stone Fruit Trees of Himachal Pradesh, India

Wasps are predominant in the Oriental and Palaearctic regions, sometimes used as dishes, and are important candidates for different ecosystems as predators and pollinators. In India, more than a dozen predatory wasp species have been reported to contribute to biological pest control. Various wasp species are invasive in some continents and pose an alarming threat to apiaries, households, and orchardists. Wasps feed on tree bark to fulfill their dietary needs but become dangerous to humans in orchards. The scientific reports on tree bark defoliation by wasps are little and scanty. A field-based survey study assessed the quantum of infestation in fruit orchards and the threat experienced by field workers in the Kangra district of Himachal Pradesh, India during the years 2022 and 2023. Wasps of species, Vespa basalis and V. auraria were encountered infesting five orchards on tree bark in Samloti (32.08° N, 76.35° E), Shahpur (32.2197° N, 76.1728° E), Nagrota (32.1054° N, 76.3789° E), and Hatwas (32.05 ° N and 76.40 ° E). Average tree per cent infestation during these years was recorded as 26.83%, 28.85%, 40.04%, 75% and 57.91% in lowland apple (Malus domestica), pear (Pyrus communis), peach (Prunus persica), losada (Cordia myxa), and sirish (Albizia lebbeck), respectively. This bark-feeding activity rendered the infested trees unfit for optimal growth, feeding on fruits, suggesting a potential threat to local orchard productivity. The noxious presence of wasps hinders activities, obstructs and stings field staff. Escalating wasp damage led farmers to adopt fermented honey bottle traps. This study is the maiden attempt to address the harmful behavior of wasps as tree bark feeders in India and may pave the way for detailed research in orchards. Thus, keeping in view the role of wasps within agricultural landscapes as detrimental agents, mindful management practices are therefore necessary.

First report of Phyllosticta capitalensis causing leaf blotch on Chinese olive (Canarium album) in Guangdong province of China.

Chinese olive (Canarium album) is a well-known plant used both as food and medicine, extensively cultivated in southern China (Kuo et al., 2016). Its production is challenged by various fungal diseases. From July to October 2023, Chinese olive plants in an approximately 400 hectare conventional hillside orchard in Chaozhou, Guangdong province, China (23°39'45.36″N, 116°37'45.48″E), where sustainable Chinese olive farming has been practiced for over a decade, exhibited leaf blotch symptoms with a disease incidence of 40% (n = 100 plants investigated). Symptoms include yellow or brown leaf blotches, followed by drying, curling, and eventual leaf drop. To identify the causal agent, seven symptomatic leaves from seven trees were collected. The symptomatic leaves were cut into pieces of about 0.3 mm2 at the necrotic lesion edges and surface disinfected. The pieces were placed on potato dextrose agar (PDA), incubated in the dark at 28°C for 5 days, then subcultured for 7 days. Four fungal isolates were obtained with an isolation frequency of 57%. Genomic DNA was extracted from the isolates. Morphological analysis and internal transcribed spacer (ITS) region sequencing revealed no observable differences among isolates. The representative isolate (LB-1) cultured on PDA was initially white, turning light grey with dense aerial mycelium after 7 days. The transparent hyphae displayed intricate branching patterns and multiple cellular structures. The conidia were single-celled, hyaline, fusiform structures measuring 3.6-7.8 × 1.17-2.83 μm (n = 50). Morphologically, the fungal isolates resembled Phyllosticta capitalensis (Wikee et al. 2013). Primers ITS1/ITS5, ACT-512F/ACT-783R, and EF1/EF2 were used to amplify the ITS region, the actin (ACT) gene, and the transcription elongation factor (TEF), respectively (Wang et al. 2023). The sequences obtained from the LB-1 isolate have been deposited in NCBI GenBank (accession nos. PP922193; PP960207 and PP960206). A phylogenetic tree was constructed based on aligned sequences (Wang et al. 2023). BLAST analysis showed LB-1 isolate shares 99.4%-100% similarity with sequences KY855645, KY855590, and KY855919 from P. capitalensis CPC27084. Based on morphological and molecular analysis, isolate LB-1 was identified as P. capitalensis. Pathogenicity assays of the LB-1 isolate were conducted on 2-year-old Canarium album seedlings. A 10⁶ spores/mL LB-1 suspension was applied to 3 mm spots on the leaves of four plants for inoculation. Controls leaves was treated with sterile water. The inoculated plants were placed in a greenhouse at 28°C with 80% humidity. The experiment was repeated 3 times. Leaves inoculated with LB-1 isolates showed blotch symptoms similar to those in the field, the controls remained healthy. Reisolation of P. capitalensis was performed confirmed by morphology and DNA sequencing, fulfilling Koch's postulates. Previous studies have shown that P. capitalensis causes fungal diseases on fruit trees such as Citrus lalifolia and Mangifera indica (Wikee et al. 2013). To our knowledge, this is the first report of Phyllosticta capitalensis causing leaf blotch on Chinese olive in China. Preliminary observations suggest it may be present in other olive-producing regions of Guangdong. It causes significant leaf wilting and shedding, potentially reduces fruit quality, and lowers overall yield. This discovery will offer valuable insights for creating effective control measures against this recently emerged severe disease.

Functional Role of Odorant-Binding Proteins in Response to Sex Pheromone Component Z8-14:Ac in Grapholita molesta (Busck)

The plum fruit moth (PFM), Grapholita funebrana, and the oriental fruit moth (OFM), G. molesta, are closely related fruit moth species that severely damage fruit trees in Rosaceae. Both species share common primary sex pheromone components Z8-12:Ac and E8-12:Ac. The secondary sex pheromone components of PFMs consist of Z8-12:OH, Z8-14:Ac, and Z10-14:Ac, while those of OFMs include Z8-12:OH and 12:OH. Previous researchers have proved that the inclusion of Z8-14:Ac and Z10-14:Ac did not augment PFM catches but inhibited OFM catches in orchards in Europe, thereby maintaining the species-specificity of the PFM sex attractant. However, which of these components, Z8-14:Ac or Z10-14:Ac, plays the major role in inhibiting OFM attraction remains unclear. In the current study, electroantennogram (EAG) assays indicated that both OFM and PFM males exhibited a moderate EAG response to Z8-14:Ac and Z10-14:Ac. Rubber septa loaded with varying ratios of Z8-14:Ac (1% to 30%) or Z10-14:Ac (5% to 110%) combined with a constant dose of Z8-12:Ac and E8-12:Ac produced diverse trapping effects. Sex attractants containing Z8-14:Ac did not significantly affect the trapping of PFM males but drastically reduced the capture of OFM males, with the reduction reaching up to 96.54%. Attractants containing more than 10% of Z10-14:Ac simultaneously reduced the number of OFM and PFM males captured. Z8-14:Ac was indispensable for maintaining the specificity of sex pheromones. Fluorescence competitive binding assays of recombinant GmolPBP2 showed the lowest Ki value (0.66 ± 0.02 μM) among the PBPs/GOBPs from OFMs, suggesting that it is the most likely target for Z8-14:Ac. Molecular dynamic simulation and site-directed mutagenesis assays confirmed that the Phe12 residue, which forms a π–alkyl interaction with Z8-14:Ac, was crucial for GmolPBP2 binding to Z8-14:Ac. In conclusion, Z8-14:Ac is vital to the specificity of PFM sex pheromones inhibiting OFM attractants when added to Z8-12:Ac and E8-12:Ac. This could be potentially used to develop species-specific sex attractants for the PFM.

Morpho-Physiological Analysis of Artocarpus lakoocha Roxb Seeds for Improved Storability

Artocarpus lakoocha Roxb., commonly known as Monkey Jack, is a tropical fruit tree native to India and valued for its diverse uses in food, fodder, and timber. This study investigates the seed storability of A. lakoocha, focusing on its morpho-physiological traits and desiccation sensitivity. Mature fruits, collected from New Delhi, were analyzed for morphological characteristics including fruit and seed size, shape, and moisture content. Seeds were found to be recalcitrant, with high moisture content (50-55%) and limited viability (7-15 days), reflecting their sensitivity to desiccation and chilling. Germination tests revealed that seeds without testa (seed coat) exhibited higher germination rates, with fresh seeds achieving 100% germination at 52% moisture content. Desiccation significantly impacted seed viability, with moisture levels reduced from 52% to 22% over 21 hours leading to decreased germination rates. Seeds desiccated to below 37% moisture content showed a notable decline in viability. Storage studies indicated that seeds stored at 15°C retained viability for up to 180 days, while those stored at 25°C, 5°C, -10°C, and -20°C lost viability more rapidly. The results underscore the recalcitrant nature of A. lakoocha seeds, characterized by high moisture content and sensitivity to desiccation. Effective storage conditions are crucial for maintaining seed viability, with 15°C proving optimal for extended storage. This research provides insights into the storage behavior of A. lakoocha seeds and emphasizes the need for conservation strategies to address the declining populations of this economically important species.

Percepción sobre la salinización del suelo en una zona vitícola de Argentina

Salt-affected soils occupy 1,000 million hectares and concentrate in arid and semi-arid regions, affecting between 20 and 30% of irrigated lands. Primary salinization is derived from natural conditions, while secondary salinization is the product of anthropic factors. Salinized agricultural plots can be abandoned and excluded from the productive system. In this context, productive restoration is an opportunity to recover these sites. Our objective is to inquire about the environmental perception of the social actors of salinization. We aim at transversally incorporating the social component in productive restoration planning. Our study case was the East Zone of the North Oasis of Mendoza, where almost half of the irrigated area is cultivated by vines, almost a quarter is abandoned land, and the rest includes olive trees, fruit trees, and vegetables. We conducted semi-structured interviews to identify the main lines of perception, which were analyzed to recognize strengths, weaknesses, opportunities, and threats for the design of productive restoration projects at a local scale. Strategic, efficient, and sustainable planning must include the social dimension.

Nitrogen monitoring and inversion algorithms of fruit trees based on spectral remote sensing: a deep review

Nitrogen monitoring and inversion algorithms of fruit trees based on spectral remote sensing: a deep review

Research Progress on the Effectiveness of Increasing Organic Fertilizer Application in Regulating Soil Quality and Crop Quality in Orchards

This article reviews the research progress on the application of organic fertilizers instead of chemical fertilizers in fruit trees, summarizes the current situation and problems of crop fertilization, and focuses on the impact of combined application of organic fertilizers on soil quality, crop growth, and fruit quality. In response to the current problems in the application of organic fertilizers in China, future research directions were discussed.

Histone modification H3K27me3 is essential during chilling-induced flowering in Litchi chinensis.

Litchi (Litchi chinensis), a prominent fruit tree in the Sapindaceae, initiates flowering in response to low autumn and winter temperatures. This study investigates the epigenetic regulation of this process, focusing on the marks histone H3 lysine 27 trimethylation (H3K27me3) and its deposition genes during the chilling-induced floral induction and initiation stages. Our genomic analysis delineated the H3K27me3 deposition landscape across the pre-floral induction (PFId), floral induction (FId), and floral initiation (FIn) stages. We identified 5,635 differentially H3K27me3-modified genes (DHGs) in buds and 4,801 DHGs in leaves. Integration of the RNA-seq and ChIP-seq datasets identified 1,001 differentially regulated genes (DRGs) in buds and 675 DRGs in leaves, offering insights into the genes potentially targeted by H3K27me3. To probe the functional role of H3K27me3, we employed GSK343, a histone H3 lysine methyltransferase inhibitor. Treatment with GSK343 during the chilling-induced flowering process led to reduced H3K27me3 deposition at the TREHALOSE-6-PHOSPHATE SYNTHASE 1 (LcTPS1) and FRIGIDA (LcFRI) loci, resulting in increased gene expression. This manipulation delayed flowering and reduced flowering rates, confirming the pivotal role of H3K27me3 in chilling-induced flowering in litchi. Gene co-expression network analysis identified SHORT VEGETATIVE PHASE 10 (LcSVP10) as a crucial regulator in litchi flowering. Overexpression of LcSVP10 in Arabidopsis thaliana delayed flowering, indicating a conserved function in flowering time control. Our results elucidate the molecular and epigenetic mechanisms that govern floral induction in litchi and highlight the potential of epigenetic modifications to regulate flowering time in horticultural plants.

Colletotrichum gloeosporioides Swiftly Manipulates the Transcriptional Regulation in Citrus sinensis During the Early Infection Stage.

Citrus spp. represent an economically important fruit tree crop worldwide. However, molecular mechanisms underlying the interaction between citrus and the Colletotrichum gloeosporioides remain largely unexplored. In this study, we analyzed the physiological and transcriptomic changes in Citrus sinensis at different stages of incubation with C. gloeosporioides. The results indicated that C. gloeosporioides infection rapidly triggered necrosis in the epicarp of C. sinensis fruits, decreased the total flavonoid contents, and suppressed the activity of catalase, peroxidase, and superoxide dismutase enzymes. Upon inoculation with C. gloeosporioides, there were 4600 differentially expressed genes (DEGs) with 1754 down-regulated and 2846 up-regulated after six hours, while there were only 580 DEGs with 185 down-regulated and 395 up-regulated between six and twelve-hours post-inoculation. Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes enrichment analysis indicated that the DEGs, which exhibited consistent up-regulation, were associated with metabolic processes and stress responses. Through Weighted Gene Co-Expression Network Analysis, 11 key genes have been identified that could potentially play a role in the transcriptional regulation of this process, including the transcription factor bHLH189. Furthermore, the infection of C. gloeosporioides had a notable effect on both the flavonoid metabolism and the metabolic pathways related to reactive oxygen species. Our findings help to understand the interaction between citrus and C. gloeosporioides and unveil how new insights into how C. gloeosporioides circumvents citrus defense mechanisms.

The Cytological Mechanism of the Peach Haploid Producing Triploid Offspring

Abstract Peach is one of the most economically valuable fruit trees. Haploid peach trees occur spontaneously at very low frequencies and they are usually highly sterile. Therefore, the haploid with partial fertility is extremely rare germplasm, which is highly valuable to genetic research and breeding programmes. In this study, we investigated the cytological mechanism underlying the fertility of a peach haploid mutant ‘9-D’ derived from a spontaneous mutation. Cytologic evaluation and flow cytometry analysis demonstrated that ‘9-D’ is a pure haploid. Scanning electron microscope analysis revealed a considerable proportion of abnormal pollen grains in ‘9-D’. Pollen viability assay by Alexander staining showed that 50.4% of pollen grains from ‘9-D’ were viable. However, the pollen germination assay showed that only 7.6% of the pollen grains could germinate normally. Investigation of the chromosomal behavior of pollen mother cells at different stages of meiosis showed that pollen mother cells of ‘9-D’ lacked the process between anaphase I and prophase II of meiosis. Various types of sporophyte morphology were observed in haploid pollen mother cells at the tetrad stage. Measurement of the diameter of pollen grains indicated the presence of pollen with 2x ploidy in ‘9-D’. The offspring of ‘9-D’ were predominantly triploid or triploid aneuploid. The triploid offspring were more likely derived from the 2x male gametophyte combined with the haploid female gametophyte, which may explain the reason why ‘9-D’ has fertility. This study not only expands our understanding of haploid fertility mechanisms, but is also useful for ploid breeding programs in peach.

Occurrence of Botrytis cinerea Causing Gray Mold on Pecan in China

Pecan (Carya illinoinensis), a globally economically significant dried fruit and woody oil tree, faces significant challenges in production and nut quality due to the rampant outbreak of severe fungal diseases. From 2020 to 2021, an extensive occurrence of a disease resembling gray mold was observed on the leaves and fruits of pecan trees in Jiangsu Province, China. Upon isolation from symptomatic samples, Botrytis cinerea was identified through morphological analysis and phylogenetic studies of the G3PDH, HSP60, and RPB2 gene sequences. Furthermore, pathogenicity tests conclusively attributed the gray mold disease observed on pecan leaves and fruits to B. cinerea. To the best of our knowledge, this is the first time that B. cinerea has been reported on pecans. These findings thus provide a basis for further research on the management of pecan gray mold.

Dynamics of Soil Physical and Chemical Properties under Fruit Tree based Agroforestry Systems in Sub-humid Agro-climatic Zone

Dynamics of Soil Physical and Chemical Properties under Fruit Tree based Agroforestry Systems in Sub-humid Agro-climatic Zone

Pomegranate plant tissue culture technique - a review

‌Background: The pomegranate, or Punica granatum L., is a member of the "Punicaceae" family, which also includes Punica protopunica and Punica granatum. Originating in Afghanistan and Iran, it has spread over Asia, Africa, and Europe's Mediterranean region. The traditional method of pomegranate propagation is laborious and time-consuming. It does not guarantee healthy, disease-free plants. Its shortcomings include poor success rates, extremely sluggish replication, and a one-year establishing period for new plants. Tissue culture is a technique that can be used to grow plants in all seasons without restrictions. Several pomegranate-growing nations have tried in vitro propagation to generate virus-free, similar-type plants, and it has been widely used for propagating many fruit trees. Research Purpose: The goal of this essay was to examine how to do plant tissue culture method and get familiar with the techniques. with an emphasis on the pomegranate micropropagation. The only aspect of plant tissue culture that may be able to get around these issues is in vitro technology. Therefore, there is plenty of room for employing micropropagation to multiply desired genotypes on a wide scale. Research Method: This review article was conducted based on scientific websites in Persian and English languages, including SID, Magiran, Google Scholar, Web of Science, and Library Studies, Results: Pomegranate plants multiply quickly through micropropagation, producing plants (clones) that are genetically similar. In a short amount of time, a single explant can yield thousands of plants. These plants match exactly. The study provides a standardized protocol for in vitro regeneration of nodal explants, which could be useful in large-scale production of uniform and disease-free plantlets. The process of plant tissue culture reduces the need for importing expensive plant seeds.

Host influence on life history traits of Ceratitis capitata Wiedemann in an arid region of Argentina.

The fruit fly Ceratitis capitata is a successful generalist due to the trade-off between different variables in its life history traits. The present study aimed to assess some life history traits of C. capitata recovered from peach, plum and fig, three key host species, under the climatic conditions of an irrigation oasis in the arid province of San Juan, Argentina. Pupal abundance, sex ratio and morphometric parameters such as pupal and adult weight, pupal volume, wingspan and head-caudal length were influenced by the host in which the larvae were reared. Sexual maturity, measured as the peak of calling (pheromone emission), was homogeneous on the sixth and seventh days after emergence, but males recovered from fig showed early calling activity on the third day after emergence. The plasticity of C. capitata to adapt its life history traits to specific nutrient variations within the larval environment allows it to colonise and establish populations where key hosts, as those studied here, are available. The knowledge of the interactions between host fruit trees and C. capitata populations provides helpful information for planning the management of local orchards and urban fruit plantations within irrigation oases, taking into consideration the concept of key hosts as peach and fig.

Genome-Wide Identification of Heat Shock Transcription Factor Family and Key Members Response Analysis to Heat Stress in Loquat

Eriobotrya japonica (loquat) is an evergreen fruit tree of the apple tribe in Rosaceae with high edible and medicinal value. The yield and quality of loquat fruits are significantly influenced by environmental stress, particularly heat stress during fruit ripening. In this study, thirty EjHSFs were identified in the loquat genome. Twenty-nine EjHSFs were unevenly distributed across sixteen chromosomes, except Chr-6. A synteny analysis revealed that twenty-six EjHSF genes had undergone duplication events. Twenty-nine EjHSF genes were found to be in sync with HSF in apples while also diverging with other Rosaceae species. A phylogenetic analysis revealed that EjHSFs could be divided into three categories, including eighteen EjHSF-A, ten EjHSF-B, and two EjHSF-C. Twenty-nine members of the EjHSF family showed high homology to those of Malus domestica and Gillenia trifoliate. A promoter analysis retrieved thirty-three CAREs that were functionally relevant and connected to the expression of HSFs. Among these, the promoters of twenty-three EjHSF genes possessed at least one STRE element that could be activated by heat shock. Eleven of these EjHSFs were highly expressed in loquat tissues, namely EjHSF-B4a, EjHSF-A4a, EjHSF-A4d, and EjHSF-C1a in roots; EjHSF-B4b in roots and inflorescence; EjHSF-C1b in stems and roots; EjHSF-A2a in three tissues; EjHSF-A8b in four tissues; and EjHSF-A4c, EjHSF-B1a, and EjHSF-B2b in six tissues. Moreover, fifteen EjHSFs were differentially expressed (eleven upregulated and four downregulated) in fruits under heat stress treatment in the color-changing period. Among them, EjHSF-A2a and EjHSF-A2b upregulated transcriptional abundance over 300 times after heat treatment; EjHSF-B2b consistently displayed an increasing trend over time; and EjHSF-B1a was significantly downregulated. Hence, these results suggest that EjHSFs take part in loquat tissue development and may be involved in the fruit’s heat stress response. These findings enhance the understanding of EjHSFs’ role within loquats and the heat stress response of its fruit and provide target genes for heat stress improvement.

Simulation optimization and experimental validation of hydraulic impact in pruning machines

Abstract This study addresses the instability caused by hydraulic shocks in fruit tree pruning machines during operation and proposes an optimization scheme that combines simulation with experimentation. We investigated the mechanisms of load shock in the hydraulic system through mathematical modeling and LS-DYNA simulation analysis. The optimal operating parameters were determined using mechanical and orthogonal analyses: a forward speed of 4 km h−1, a pruning tool angle of 60°, and a saw blade rotation speed of 1200 r min−1. Additionally, we employed AMESim simulation to optimize the hydraulic system of the pruning machine by designing a dual accumulator configuration to effectively suppress hydraulic shocks. We validated the optimized system through simulations and experimental tests, demonstrating significant reductions in pressure fluctuations, with cutting efficiency improved by 30% and pressure fluctuations reduced by 26.7%. This study not only presents an innovative dual accumulator design but also validates its effectiveness in pruning machines through simulations and experiments, thereby enhancing the operational efficiency and stability of agricultural machinery.