Opuntia Ficus-indica Fruit Research Articles

Positive impact of indicaxanthin from Opuntia ficus-indica fruit on high-fat diet-induced neuronal damage and gut microbiota dysbiosis.

Indicaxanthin is a betalain that is abundant in Opuntia ficus-indica orange fruit and has antioxidative and anti-inflammatory effects. Nevertheless, very little is known about the neuroprotective potential of indicaxanthin. This study investigated the impact of indicaxanthin on neuronal damage and gut microbiota dysbiosis induced by a high-fat diet in mice. The mice were divided into three groups according to different diets: the negative control group was fed a standard diet; the high-fat diet group was fed a high-fat diet; and the high-fat diet + indicaxanthin group was fed a high-fat diet and received indicaxanthin orally (0.86 mg/kg per day) for 4 weeks. Brain apoptosis, redox status, inflammation, and the gut microbiota composition were compared among the different animal groups. The results demonstrated that indicaxanthin treatment reduced neuronal apoptosis by downregulating the expression of proapoptotic genes and increasing the expression of antiapoptotic genes. Indicaxanthin also markedly decreased the expression of neuroinflammatory proteins and genes and inhibited high-fat diet-induced neuronal oxidative stress by reducing reactive oxygen and nitrogen species, malondialdehyde, and nitric oxide levels. In addition, indicaxanthin treatment improved the microflora composition by increasing the abundance of healthy bacterial genera, known as producers of short-chain fatty acids (Lachnospiraceae, Alloprovetella, and Lactobacillus), and by reducing bacteria related to unhealthy profiles (Blautia, Faecalibaculum, Romboutsia and Bilophila). In conclusion, indicaxanthin has a positive effect on high-fat diet-induced neuronal damage and on the gut microbiota composition in obese mice.

The remarkable impact of Opuntia Ficus Indica fruit administration on metabolic syndrome: Correlations between cognitive functions, oxidative stress and lipid dysmetabolism in the high-fat, diet-fed rat model

BackgroundA wealth of evidence underscores the bioactive properties of nutraceuticals and functional foods in addressing oxyinflammatory-based diseases with implications at both peripheral and central levels. Opuntia ficus-indica (OFI) is well-documented for its health-promoting attributes, though its fruit (OFIF) remains relatively understudied. Not only poses Metabolic Syndrome (MetS) cardiometabolic risks but also contributes significantly to cognitive impairment, especially in crucial brain areas such as hippocampus and hypothalamus. MethodsFollowing 8 weeks of HFD to induce MetS, rats received OFIF oral supplementation for 4 weeks to evaluate cognitive and affective modifications using behavioural paradigms, i.e. open field, burrowing, white-dark box, novelty-suppressed feeding, and object recognition tests. Our investigation extended to biochemical evaluations of lipid homeostasis, central and peripheral oxidative stress and neurotrophic pathways, correlating these measures together with circulating leptin levels. ResultsOur data revealed that OFIF modulation of leptin positively correlates with systemic and brain oxidative stress, with markers of increased anxiety-like behaviour and impaired lipid homeostasis. On the other hand, leptin levels reduced by OFIF are associated with improved antioxidant barriers, declarative memory and neurotrophic signalling. DiscussionThis study underscores OFIF neuroactive potential in the context of MetS-associated cognitive impairment, offering insights into its mechanisms and implications for future therapeutic strategies.

Extracellular Vesicles Derived from Opuntia ficus-indica Fruit (OFI-EVs) Speed Up the Normal Wound Healing Processes by Modulating Cellular Responses.

Plant-derived extracellular vesicles (EVs) have been recognized as important mediators of intercellular communication able to transfer active biomolecules across the plant and animal kingdoms. EVs have demonstrated an impressive array of biological activities, displaying preventive and therapeutic potential in mitigating various pathological processes. Indeed, the simplicity of delivering exogenous and endogenous bioactive molecules to mammalian cells with their low cytotoxicity makes EVs suitable agents for new therapeutic strategies for a variety of pathologies. In this study, EVs were isolated from Opuntia ficus-indica fruit (OFI-EVs) and characterized by particle size distribution, concentration, and bioactive molecule composition. OFI-EVs had no obvious toxicity and demonstrated a protective role in the inflammatory process and oxidative stress in vitro model of chronic skin wounds. The results demonstrated that pretreatment with OFI-EVs decreased the activity and gene expression of pro-inflammatory cytokines (IL-6, IL-8, and TNF-α) in the LPS-stimulated human leukemia monocytic cell line (THP-1). Furthermore, OFI-EVs promote the migration of human dermal fibroblasts (HDFs), speeding up the normal wound healing processes. This study sheds light, for the first time, on the role of OFI-EVs in modulating important biological processes such as inflammation and oxidation, thereby identifying EVs as potential candidates for healing chronic cutaneous wounds.

Identification and molecular docking of xanthine oxidase and α-glucosidase inhibitors in Opuntia ficus-indica fruit.

Opuntia ficus-indica fruit (OFI) is rich in bioactive compounds, which can promote human health. In this work, the purified OFI extract was prepared from OFI and its bioactivities were investigated. Xanthine oxidase (XOD) and α-glucosidase (α-Glu) inhibitors of the purified OFI extract were screened and identified by bio-affinity ultrafiltration combined with UPLC-QTRAP-MS/MS technology. The inhibitory effect of these inhibitors on enzymes were verified, and the potential mechanism of action and binding sites of inhibitors with enzymes were revealed based on molecular docking. The results showed that the total phenolic content of the purified OFI extract was 355.03mg GAE/g DW, which had excellent antioxidant activity. Additionally, the extract had a certain inhibitory effect on XOD (IC50=199.00±0.14µg/mL) and α-Glu (IC50=159.67±0.01µg/mL). Seven XOD inhibitors and eight α-Glu inhibitors were identified. Furthermore, XOD and α-Glu inhibition experiments in vitro confirmed that inhibitors such as chlorogenic acid, taxifolin, and naringenin had significant inhibitory effects on XOD and α-Glu. The molecular docking results indicated that inhibitors could bind to the corresponding enzymes and had strong binding force. These findings demonstratethat OFI contains potential substances for the treatment of hyperuricemia and hyperglycemia.

In Vitro Screening and Lipid-Lowering Effect of Prickly Pear (Opuntia Ficus-Indica L. Mill.) Fruit Extracts in 3T3-L1 Pre-Adipocytes and Mature Adipocytes

Opuntia ficus-indica fruits have been widely used due to their nutritional composition and beneficial effects on health, particularly against chronic diseases such as diabetes, obesity, cardiovascular diseases and cancer, among others. In recent years, prickly pear peel and pulp extracts have been characterised, and a high number of bioactive compounds have been identified. This study aimed to analyse the triglyceride-lowering effect of prickly pear peel and pulp extracts obtained from fruits of three varieties (Pelota, Sanguinos, and Colorada) in 3T3-L1 maturing and mature adipocytes. At a concentration of 50 µg/mL, peel extracts from Colorada reduced triglyceride accumulation in pre-adipocytes and mature adipocytes. Additionally, at 25 µg/mL, Pelota peel extract decreased triglyceride content in mature adipocytes. Moreover, maturing pre-adipocytes treated with 50 and 25 µg/mL of Sanguinos pulp extract showed a reduction of triglyceride accumulation. In addition, the lipid-lowering effect of the main individual betalain and phenolic compounds standards were assayed. Piscidic acid and isorhamnetin glycoside (IG2), found in Colorada peel extract, were identified as the bioactive compounds that could contribute more notably to the triglyceride-lowering effect of the extract. Thus, the betalain and phenolic-rich extracts from Opuntia ficus indica fruits may serve as an effective tool in obesity management.

Therapeutic Intervention of Opuntia Ficus Indica (L.) Fruit and Seed Powder against CCl4-Induced Acute Liver Injury in Wistar Rats

Therapeutic Intervention of Opuntia Ficus Indica (L.) Fruit and Seed Powder against CCl4-Induced Acute Liver Injury in Wistar Rats

Composition of discarded Sicilian fruits of Opuntia ficus indica L.: Phenolic content, mineral profile and antioxidant activity in peel, seeds and whole fruit.

Sicily (Italy) is the second producer of Opuntia ficus-indica (OFI) fruits after Mexico. To date, huge quantities of fruit are discarded during the selection for the fresh market, generating a large amount of by-product to be valorized. This study aimed to investigate on the composition of OFI discarded fruits from the main Sicilian productive areas, over two harvesting periods. Peel, seeds and whole fruit samples were characterized in terms of minerals and phenolic compounds through ICP-OES and HPLC-DAD-MS. Potassium, calcium and magnesium were the most abundant elements and peel samples showed the highest values. Seventeen phenolic compounds were detected in peel and whole fruit, including flavonoids, phenylpyruvic and hydroxycinnamic acids, while only phenolic acids were found in the seeds. A multivariate chemometric approach highlighted a correlation between the mineral and phenolic content and the different parts of the fruit as well as a significant influence of productive area.

Purification and Characterization of Polyphenol Oxidase in the Fruits of Opuntia ficus-indica.

Firstly, polyphenol oxidase (PPO) was purified from the fruits of Opuntia ficus-indica using Sepharose 4B-L-tyrosine-p-aminobenzoic acid affinity chromatography, and the enzyme was characterized. The PPO was purified 20.59-fold. Thereafter, PPO was performed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The kinetic parameters, optimum pHs, and optimum temperatures were investigated for three substrates. Opuntia ficus-indica PPO's optimum pH and optimum temperature were 9.0 and 20 °C; 7.5 and 20 °C; and 7.5 and 30 °C, respectively, when using pyrogallol, catechol, and 4-methyl catechol as substrates. For the pyrogallol, catechol, and 4-methyl catechol, the Km, Vmax, and Vmax/Km values were determined as 16.67 mM, 833.33 U/mLmin, and 50 U/mLminmM; 6.33 mM, 126.58 U/mLmin, and 20 U/mLminmM; and 5.38 mM, 107.53 U/mLmin, and 20 U/mLminmM, respectively. As a result, pyrogallol was a more appropriate substrate than catechol and 4-methyl catechol for the PPO from Opuntia ficus-indica.

Nutritional Composition and Phytochemical Evaluation of Some Selected Wild Edible Plants in Tach Gaint District, Northwestern Ethiopia.

The objective of the study was to evaluate the nutritional, mineral, and phytochemical analyses of some selected wild edible plants from Tach Gaint District, Northwest Ethiopia. Proximate composition parameters (moisture, ash, crude fibre, crude fat, crude protein, carbohydrate, and energy) were evaluated using the methods of the Association of Official Analytical Chemists, and elemental analysis was performed using the atomic absorption spectroscopy technique. Results from the nutritional analysis show that Erucastrum abyssinicum leaves had the highest crude protein content (17.47 ± 0.03 g/100 g), followed by Amaranthus graecizans (14.97 ± 0.03 g/100 g). The maximum moisture content (40.8 ± 0.00 g/100 g) and ash content (24.70 ± 0.15 g/100 g) were reported in the leaves of Amaranthus graecizans. The young shoots of Rumex abyssinicus had the highest crude fat content (14.07 ± 0.03 g/100 g) and the highest fibre content (34.70 ± 0.25 g/100 g), while the fruits of Opuntia ficus-indica had the highest amount of utilisable carbohydrate (44.4 ± 0.00 g/100 g) and the estimated energy value (326.4 ± 0.00 Kcal/100 g). Calcium was detected in considerable proportions (754.9 ± 0.23 mg/100 g) followed by iron (31.63 ± 0.03 mg/100 g) in Urtica simensis leaves and zinc content (3.09 ± 0.02 mg/100 g) in young shoots of Rumex abyssinicus. Qualitative phytochemical screening, alkaloids, phenols, flavonoids, triterpenes, saponins, and tannins were found in the methanolic extract of the plants. The results of this study suggest that the consumption of such nutrient-rich wild edible plants could help add a remarkable amount of nutrient and mineral in the human diet.

Adverse effects of MSG and aspartame on the liver of Female Albino Rats and their offspring and the possible ameliorative role Opuntia ficus-indica Fruit

Adverse effects of MSG and aspartame on the liver of Female Albino Rats and their offspring and the possible ameliorative role Opuntia ficus-indica Fruit

Effectiveness of Opuntia ficus indica (cactus) fruit juice for sludge conditioning.

The possibility of using Opuntia ficus indica fruit juice (OFIFJ) as a bioflocculant for conditioning the synthetic kaolin sludge and sewage sludge (region Oran, Algeria, and Pau, France) was studied. Turbidity of the supernatant, dryness of the sludge cake, and total time of filtration (TTF) were examined parameters. Using vacuum filtration, lime was also tested as a chemical conditioner and gives good results on Lescar (France) sewage sludge in terms of cake's dryness, filtrate quality, turbidity (13.54%), and total time of filtration (TTF = 85.29%), comparing to the industrial polymer (Sedifloc 408C; turbidity; 8.33% and TTF: 2.94%). For the sewage sludge of Oran (Algeria), the results obtained with OFIFJ were compared to those obtained with the cladodes juice of the same plant OFIC, and also with a cationic polymer (Superfloc 8396). For an optimum dosage, it showed that OFIFJ has a flocculation activity as same as the cladodes juice OFIC for sludge conditioning and gives better results in terms of turbidity (dosage of 22.4g/kg DM: 3.7 NTU for OFIC, dosage of 8.36: 3.63 NTU for OFIFJ. Dryness was enhanced from 14.91 to 22.93% (OFIC 16g/kg DM) and to 24.48% (OFIF 20.9g/kg DM) but for TTF, we found the opposite. In fact, this plant showed to be an available, biodegradable, and non-toxic flocculant. For kaolin synthetic sludge (30%), the optimum dosages of those conditioners were found to be 0.066gkg-1 for OFIC, comparing between vacuum filtration and filtration compression; turbidity was enhanced for both techniques, contrary to dryness. Concerning the Oran city sewage sludge, both turbidity and dryness were optimized. Same thing for the France sewage sludge, all the studied parameters were enhanced with the two studied bioflocculants.

Dyeing of Wool Yarn with Aqueous Extract of Opuntia ficus-indica Fruit Containing Betalain Colorant Employing Ascorbic Acid as a Bio-mordant Under Varied Circumstances of Dyeing

Dyeing of Wool Yarn with Aqueous Extract of Opuntia ficus-indica Fruit Containing Betalain Colorant Employing Ascorbic Acid as a Bio-mordant Under Varied Circumstances of Dyeing

Spray-Drying Microencapsulation of Andean Blueberry (Vaccinium meridionale Sw.) Anthocyanins Using Prickly Pear (Opuntia ficus indica L.) Peel Mucilage or Gum Arabic: A Comparative Study.

The recovery of byproducts from the food industry is a promising approach to obtain hydrophilic biopolymers with potential health benefits. In this work, the mucilage obtained from the peel of the Opuntia ficus-indica (OFI) fruit was compared with gum arabic (GA) as wall materials for the microencapsulation of Colombian blueberry anthocyanins, using the spray-drying process. For both types of microencapsulates, the following were determined: anthocyanin content (UV-vis and HPLC/MS-MS), total dietary content (enzymatic-gravimetric method), antioxidant activity (ORAC), color (CIELab parameters), morphology (SEM and particle size), and thermal behavior (DSC/TGA). Six different anthocyanins were identified by HPLC/MS-MS in the non-lyophilized Andean blueberry sample (LABP) and in the OFI-mucilage and GA microcapsules. OFI mucilage, compared to GA, favors the formation of larger spherical particles, a smoother surface without cracks, and greater thermal stability. The higher anthocyanin retention capacity in OFI microcapsules leads to higher antioxidant capacity and red coloration for this biomaterial. Consequently, the microencapsulation of anthocyanins with mucilage from the peel of the OFI fruit is proposed as a promising alternative for the protection and incorporation of this natural dye with high antioxidant capacity and dietary fiber content in new functional food/cosmetic formulations, while giving added value to the natural byproducts of OFI.

Assessment of technological and functional features of Lactiplantibacillus and Fructobacillus strains isolated from Opuntia ficus-indica fruits

Opuntia ficus-indica fruits are outstanding for their sensory attributes and multiple health benefits. However, this fruit is highly perishable, and substantial efforts have been carried out to extend its shelf-life. Lactic fermentation of fruits by-products using autochthonous bacteria arises as relevant technology for preserving vegetables and their by-products. In this study, autochthonous Lactiplantibacillus plantarum and Fructobacillus fructosus strains previously selected from Opuntia ficus-indica fruits of Northwestern Argentina were characterized according to their technological and functional properties to select the most suitable for the production of fermented cactus pear products. L. plantarum strains showed better acidifying activity, decreasing the pH of the juice by about 2.9 units in 24 h. All the strains studied produced lactic and propionic acids, and L.plantarum S-811 and S-TF2 strains and F. fructosus S-TF7 strain were the better lactic acid producers, with values around 9.5 g/l. These strains also displayed antimicrobial activities against undesirable pathogen bacteria, showed a safety profile typical of lactic acid bacteria, and the juice fermented with these strains preserves the phenolic compounds content and antioxidant activity of unfermented juice. The obtained results showed the potential of L.plantarum S-811 and S-TF2, and F. fructosus S-22 for their use as starters for the fermentation of cactus pear by-products, standing out L.plantarum S-811 for its potentiality to elaborate a fermented cactus pear beverage. The cactus pear juice fermented by L.plantarum S-811 showed physicochemical and microbiological stability that favors juice shelf-life. Besides, fermentation conferred distinctive sensory features to the cactus pear juice without influencing consumers' overall acceptability.

Opuntia ficus-indica Fruit: A Systematic Review of Its Phytochemicals and Pharmacological Activities.

The use of Opuntia ficus-indica fruits in the agro-food sector is increasing for a multiplicity of players. This renewed interest is, in part, due to its organoleptic characteristics, nutritional value and health benefits. Furthermore, industries from different sectors intend to make use of its vast array of metabolites to be used in different fields. This trend represents an economic growth opportunity for several partners who could find new opportunities exploring non-conventional fruits, and such is the case for Opuntia ficus-indica. O. ficus-indica originates from Mexico, belongs to the Cactaceae family and is commonly known as opuntia, prickly pear or cactus pear. The species produces flowers, cladodes and fruits that are consumed either in raw or in processed products. Recent publications described that consumption of the fruit improves human health, exhibiting antioxidant activity and other relevant pharmacological activities through enzymatic and non-enzymatic mechanisms. Thus, we provide a systematic, scientific and rational review for researchers, consumers and other relevant stakeholders regarding the chemical composition and biological activities of O. ficus-indica fruits.

Evaluation of Turbidity and Color Removal in Water Treatment: A Comparative Study between Opuntia ficus-indica Fruit Peel Mucilage and FeCl3.

Natural coagulants derived from by-products have gained popularity as sustainable alternatives to inorganic coagulants in water/wastewater treatment due to their abundant availability, biodegradability, low cost, easy disposal and low sludge volumes. In this study, the mucilage obtained from the peel of Opuntia ficus-indica fruit was evaluated as a biocoagulant for treating synthetic turbid water and compared with a traditional chemical coagulant (FeCl3). The effects of coagulant dosage and pH on the turbidity and color-removal efficiency of synthetic turbid water were analyzed. To estimate the coagulation mechanism, the flocs produced under optimal values were characterized structurally (FTIR and zeta potential) and morphologically (SEM). The optimal condition for the removal of turbidity and color was a coagulant dose of 12 mg/L at pH 13. For the optimal values, the biocoagulant and the FeCl3 presented a maximum removal of 82.7 ± 3.28% and 94.63 ± 0.98% for turbidity and 71.82 ± 2.72% and 79.94 ± 1.77% for color, respectively. The structure and morphology of the flocs revealed that the coagulation mechanism of the mucilage was adsorption and bridging, whereas that of FeCl3 was charge neutralization. The results obtained showed that the mucilage could be used as an alternative coagulant to replace FeCl3.

Novel green synthesis of N-doped carbon dots from fruits of Opuntia ficus Indica as an effective catalyst for the photocatalytic degradation of methyl orange dye and antibacterial studies

• We have prepared Undoped and N-doped C-dots from Opuntia ficus Indica plant extract. • The synthesized Undoped and N-doped C-dots exhibit, the green fluorescence. • We found higher photocatalytic activity in N-doped C-dots than Undoped C-dots. • N-doped C-dots have utilized as photocatalytic for the degradation of Methyl orange dye. • Further we have study them anti-bacterial activity using gram negative and gram positive bacterias. A carbon dot is an attractive material for the photocatalytic degradation of organic pollutant. Moreover it’s extensively used as a material to control the growth of micro organisms. Herein we are reporting the synthesis of Undoped and N-doped carbon dots (N-CDs) derived from Opuntia Ficus Indica (O.F.Indica) fruits. Further we have well characterized by UV-Vis, FT-IR, XPS spectral, HR-TEM, Raman and XRD techniques. The synthesized Undoped and N-CDs are stable for 3 months. Finally we have found the N-CDs shows the higher photocatalytic activity then Undoped C-dots, due to presence of N-atom. We also have discussed the possible degradation mechanism of Methyl Orange (MO). Finally the N-CDs was utilized their antibacterial activities. We used Streptococcus Pyogenes, Staphylococcus Aureus , Klebsiella Pneumoniae, and Shigella Flexneri. Among them we found Streptococcus Pyogenes a bacterium was largely controlled the growth by our N-CDs.

Fabrication of a novel bioactive chitosan based biocomposite from Opuntia ficus-indica fruit gum and evaluation of anticancer activity in bone MG63 cancer and L929 normal cell lines

In this paper, a novel bioactive Chitosan-Lignin/n-Hydroxyapatite/Opuntia ficus-indica -Fruit gum (Cs-Lg/n-Hap/OFI-Fg) biocomposite was fabricated. The as-obtained biocomposite properties are analyzed by optical and structural investigation. The biological properties like antibacterial, antioxidant, and anticancer ability were evaluated for the biocomposites. The FT-IR spectrum result shows the biocomposites possess significant intermolecular interaction with biomolecules. The powder XRD result indicated the as-obtained Cs-Lg/n-Hap/OFI-Fg biocomposites are crystalline in nature. The microhardness test shows the addition of fruit gum increases the mechanical strength of the biocomposite. The thermal stabilities of the as-obtained Cs-Lg/n-Hap/OFI-Fg biocomposite were higher than to Cs-Lg/n-Hap composite as revealed by TGA analysis. The SEM images show an addition of fruit gum leads to the formation of spherical-shaped discrete particle morphology evidenced by TEM analysis. The antioxidant and antibacterial activity of bioactive biocomposite shows better activity due to the presence of numerous biomolecules. The MTT assay result revealed significant activity in as-obtained Cs-Lg/n-Hap/OFI-Fg biocomposite compared to Cs-Lg/n-Hap composite against MG63 bone cells and L929 cells due to the active biomolecules extant in fruit gum. The anticancer results revealed that 5 µg/ml concentration biocomposite is a significant role against bone cancer cells which proves that the as-obtained composites could be more valuable for the treatment of bone deficiency.

Modulation of Human Motor Cortical Excitability and Plasticity by Opuntia Ficus Indica Fruit Consumption: Evidence from a Preliminary Study through Non-Invasive Brain Stimulation.

Indicaxanthin (IX) from Opuntia Ficus Indica (OFI) has been shown to exert numerous biological effects both in vitro and in vivo, such as antioxidant, anti-inflammatory, neuro-modulatory activity in rodent models. Our goal was to investigate the eventual neuro-active role of orally assumed fruits containing high levels of IX at nutritionally-relevant amounts in healthy subjects, exploring cortical excitability and plasticity in the human motor cortex (M1). To this purpose, we applied paired-pulse transcranial magnetic stimulation and anodal transcranial direct current stimulation (a-tDCS) in basal conditions and followed the consumption of yellow cactus pear fruits containing IX or white cactus pear fruits devoid of IX (placebo). Furthermore, resting state-functional MRI (rs-fMRI) preliminary acquisitions were performed before and after consumption of the same number of yellow fruits. Our data revealed that the consumption of IX-containing fruits could specifically activate intracortical excitatory circuits, differently from the placebo-controlled group. Furthermore, we found that following the ingestion of IX-containing fruits, elevated network activity of glutamatergic intracortical circuits can homeostatically be restored to baseline levels following a-tDCS stimulation. No significant differences were observed through rs-fMRI acquisitions. These outcomes suggest that IX from OFI increases intracortical excitability of M1 and leads to homeostatic cortical plasticity responses.

Opuntia ficus indica (L.) fruit extract alleviates oxidative stress through activation of dual oxidases and Keap1/Nrf2 signaling cascades in high-fat-diet associated atherosclerosis rats.

Atherosclerosis is a metabolic disorder characterized by chronic inflammation associated with progressive thickening and hardening of the large to medium-sized arteries due to plaque formation. The study aims to evaluate the antioxidative, anti-inflammatory, and hypolipidemic efficacy of Opuntia ficus-indica (OFI) fruit extract against the high-fat-diet associated atherosclerotic rat model. In-vitro qualitative and quantitative phytochemical screening of OFI fruit extract revealed the significant presence of total phenolic content and total flavonoid contents. In-vitro antioxidant activity of fruit extract was determined through 2,2-diphenyl-1-picrylhydrazyl and FRAP assays that have shown their protective efficacy against the overproduction of reactive oxygen species. Results revealed that the level of total oxidant stress was significantly (P<0.05) reduced and down expression levels of dual oxidases (Duox, Duoxa-1, and Duox-2) in all the treatment groups (I, II, III) as compared with positive control were observed. The total antioxidant capacity was significantly (P<0.05) increased in all treatment groups in comparison with the positive control group and higher expression level of the Nrf-2 signaling pathway (Nfe-212, NFR-1, and Keap-1) was observed in all the treatment groups compared with the positive control group. Histopathological examination of the aorta showed that high-fat diet markedly increased endothelial lining and thickness of tunica media and adventitia, with irregular media segments having wavy laminae, and a significant increase in entropy of fibers disposition was observed. Conclusively, OFI fruit extract has shown promising protective, anti-oxidative, and anti-inflammatory efficacy through the restoration of normal parenchyma in high-fat dieting-associated oxidative stress and endothelial inflammation.