Fruit Supplementation Research Articles

Lifestyle, dietary, and medical history factors associated with pancreatic cancer risk in Ontario, Canada

Pancreatic adenocarcinoma has one of the worst survival rates of all the cancers. Established risk factors for this malignancy are smoking, body mass index (BMI) and family history of pancreatic cancer. Findings are inconsistent regarding pancreatitis, diabetes, allergies, intake of fruit, vegetables, red meat, alcohol, caffeine, vitamin C, calcium, and folate supplements. Possible pancreatic cancer risk factors were evaluated within the population-based Ontario Pancreas Cancer Study. Pathologically confirmed pancreatic cancer cases (n = 422) were identified from the Ontario Cancer Registry between 2003 and 2007. Controls (n = 312) were recruited through random digit dialing. Data were collected using self-administered questionnaires. Multivariate logistic regression was used to obtain odds ratios. Smoking, BMI, family history of pancreatic cancer, and caffeine were significantly associated with increased pancreatic cancer risk, while fruit intake and allergies significantly decreased risk. No other significant associations were observed in the multivariate model. Effect modification by smoking status was suggested for caffeine, family history of pancreatic cancer, BMI, and fruit. This study further clarifies the association between several lifestyle, dietary and medical history factors, and pancreatic cancer risk, many of which are potentially modifiable. Possible effect modification by smoking status should be further explored in future etiologic studies.

Serum β-Glucuronidase Activity in Response to Fruit and Vegetable Supplementation: A Controlled Feeding Study

Fruit and vegetable (F&V) intake may lower the risk of some cancers. One hypothesized, but understudied, chemopreventive mechanism is that plant food constituents inhibit beta-glucuronidase, an acid hydrolase that deconjugates glucuronides. We conducted a crossover feeding trial in 63 healthy women and men ages 20 to 40 years to examine the effect of diet on serum beta-glucuronidase activity. Participants were randomized to two 2-week experimental diets with an intervening washout period: a diet high in selected citrus fruit, crucifers, and soy (F&V) and a diet devoid of fruits, vegetables, and soy (basal). Serum beta-glucuronidase activity was measured during the preintervention, F&V, and basal periods. Linear mixed models were used to obtain effect estimates and 95% confidence intervals (95% CI). We observed statistically significantly higher beta-glucuronidase activity during the F&V than the basal diet (ratio, F&V versus basal diet, 1.09; 95% CI, 1.05-1.13; P < 0.01). These results were probably due to decreased beta-glucuronidase activity during the basal diet (ratio, basal period versus preintervention, 0.93; 95% CI, 0.87-0.98; P = 0.01) rather than increased enzyme activity during the F&V diet (ratio, F&V period versus preintervention, 1.01; 95% CI, 0.96-1.06; P = 0.64). Response to the experimental diet did not differ by sex (P(interaction) = 0.30), but there was a suggestion of a short-term diet effect at 8 versus 15 days (P(interaction) = 0.06). This intervention of selected F&V did not lower beta-glucuronidase activity. Further investigation is needed regarding what other foods and phytochemicals may influence beta-glucuronidase activity and effect modifiers of this relation.

Feeding Acacia etbaica and Dichrostachys cinerea fruits to smallholder goats in northern Ethiopia improves their performance during the dry season

Acacia etbaica Schweinf. and Dichrostachys cinerea (L.) Wight et Arn. are among the common woody browse plants that naturally grow in many arid and semi-arid rangelands in sub Saharan Africa. An experiment was conducted to compare the voluntary dry matter intake, body weight gain, nitrogen balance, carcass composition and sensory attributes of goats supplemented with different levels of A. etbaica and D. cinerea fruits. Average initial body weight of the goats was 20.4 kg (± 0.8SE) and received one of the following fruit supplementation regimes for 120 days (on body weight basis, n = 4 goats per group): (1) Control, no supplement; (2) 0.5% of A. etbaica; (3) 0.5% of D. cinerea; (4) 1.0% of A. etbaica; (5) 1.0% of D. cinerea; (6) 1.5% of A. etbaica; (7) 1.5% of D. cinerea. All groups were allowed to graze/browse during the day. Compared to A. etbaica, D. cinerea fruits contained higher amounts of crude protein (182 vs 135 g/kg DM), metabolizable energy (11 vs 8.4 MJ/kg DM), and in vitro dry matter digestibility coefficient (0.7 vs 0.6). The concentrations of acid detergent fiber (ADF), sulphuric acid solubulized lignin, and tannin (soluble and condensed) were lower ( P < 0.05) in D. cinerea than in A. etbaica fruits. A. etbaica fruits, however, contained higher amounts of Na, K, Fe, and Zn concentrations than D. cinerea fruits. Dry matter intake was markedly higher in supplemented groups than in the control group without supplement. Similarly, body weight gain in the control group was negative and lower ( P < 0.05) than any of the supplemented groups. During the course of the experimental period (120 days) the group placed in the 1.5% D. cinerea fruit diet gained 2.6 kg whereas the control group lost 2.0 kg. Thus, the difference between these two groups was 4.6 kg, which suggests about 22.5% increment of the goats' initial body weight. N retention was negative for the control group and varied little among the remaining treatment groups. Dressing percent increased with increased level of either type of fruit supplement, the highest (51.8%) being achieved at the 1.5% D. cinerea level. There was no difference in bone tissue across treatment groups. D. cinerea fruit intake also resulted in juicy/watery flavor and tender carcass. D. cinerea fruits appeared to have no adverse effects when included at the highest level (1.5%) and could be collected and stored as dry season supplement to smallholder goats. This study shows that poor farmers can increase goat performance substantially through supplement feeding with wild fruits. Areas in Ethiopia originally set aside for provision of ecological services and biodiversity protection can also be used for wild fruit production and feeding of small stock. The effect of increasing D. cinerea fruits above the 1.5% body weight level should be a focus of future investigation. Further work on deactivation mechanism of tannins is required for A. etbaica fruits included at levels higher than 1.0%.

Voice as Materialistic Principle

In this reply, three core notions from Schurmann’s comment The materiality of the abstraction “voice” are picked out and discussed: the ethos, the so called trialogical self and vocality as principle. The notion of ethos is explored through the word’s history, coming to say that ethos develops in relation to a certain geographical place and is determined by the social practices of a historically situated community. Thus, the concept allows to see how current scientific activities are rooted in reality, taken in the socio-cultural sense. The “trialogical self” is discussed in its implications, leading to firmly retain “dialogical self” as sign of a movement leading through the other to self. Schurmann’s distinction of voice as phenomenon and vocality as principle is seen as fruitful supplement to own ideas, especially as it highlights the fact that it is the act of uttering in different modes which matters for a language notion based in addressivity.

Berry Fruit Supplementation and the Aging Brain

The onset of age-related neurodegenerative diseases such as Alzheimer's or Parkinson's disease, superimposed on a declining nervous system, could exacerbate the motor and cognitive behavioral deficits that normally occur in senescence. In cases of severe deficits in memory or motor function, hospitalization and/or custodial care would be a likely outcome. This means that unless some way is found to reduce these age-related decrements in neuronal function, health-care costs will continue to rise exponentially. Thus, it is extremely important to explore methods to retard or reverse age-related neuronal deficits, as well as their subsequent behavioral manifestations, to increase healthy aging. In this regard, consumption of diets rich in antioxidants and anti-inflammatory polyphenolics, such as those found in fruits and vegetables, may lower the risk of developing age-related neurodegenerative diseases. Research suggests that the polyphenolic compounds found in berry fruits, such as blueberries and strawberries, may exert their beneficial effects either through their ability to lower oxidative stress and inflammation or directly by altering the signaling involved in neuronal communication, calcium buffering ability, neuroprotective stress shock proteins, plasticity, and stress signaling pathways. These interventions, in turn, may exert protection against age-related deficits in cognitive and motor function. The purpose of this paper is to discuss the benefits of these interventions in rodent models and to describe the putative molecular mechanisms involved in their benefits.

Association between coffee consumption and markers of inflammation and cardiovascular function during mental stress

Coffee is widely consumed in the Western diet and therefore has important implications for public health. Research findings pertaining to the effects of coffee consumption on cardiovascular health are conflicting, and the role of caffeine is not clear. To examine the relationship between coffee intake, inflammation and cardiovascular function at baseline and during mental stress, both cross-sectionally and after a 4-week period of withdrawal of coffee during which intake of caffeine was maintained. Eighty-five healthy, non-smoking men with varying coffee-drinking habits were recruited. Blood pressure, heart rate, and markers of inflammation [C-reactive protein (CRP), von Willebrand factor antigen (vWF)], were measured at baseline and during mental stress. These measures were repeated after a 4-week period of withdrawal of coffee, during which intake of caffeine was maintained. Habitual levels of coffee and caffeine consumption were assessed from a self-reported questionnaire, and saliva samples for the analysis of caffeine concentrations were collected regularly throughout the period of withdrawal, to confirm compliance. Multiple linear regression analysis of pre-withdrawal data, adjusted for age, body mass index and intake of tea, red wine, fruit, vegetables, oily fish and dietary supplements revealed that coffee consumption was positively related to baseline systolic blood pressure, and increased heart rate and vWF responses to mental stress. Four weeks after withdrawal of coffee, the heightened vWF and heart rate responses to stress in habitual coffee drinkers persisted, whereas baseline systolic blood pressure had decreased. Total caffeine intake was unrelated to any measures of physiological function. Habitual coffee consumption is associated with heightened acute vascular inflammatory responses to mental stress, although these effects are not affected by short-term abstinence from coffee. These findings suggest that the relationship between coffee and markers of cardiovascular risk may be explained by residual or unmeasured confounding factors.

Efficiency of apples, strawberries, and tomatoes for reduction of oxidative stress in pigs as a model for humans

Objective The present study investigated the potency of apples, strawberries, and tomatoes in decreasing oxidative stress in pigs as a model for humans. Methods Forty-eight growing pigs were individually penned in metabolic cages and divided into six groups. All groups received isocaloric daily rations composed of a basal diet supplemented with starch, linseed oil, linseed oil and apples, linseed oil and strawberries, linseed oil and tomatoes, or linseed oil, apples, strawberries and tomatoes. Oxidative stress was evaluated at the beginning and at the end of the 22-d experimental period by measuring the degree of DNA damage in mononuclear blood cells, the concentration of malondialdehyde (MDA) in plasma, the MDA excretion rate, the concentration of tocopherols in plasma, plasma total antioxidant status, and erythrocyte glutathione peroxidase concentration. Results The results confirmed that the linseed oil diet significantly increased MDA formation in the body and DNA damage to mononuclear blood cells in comparison with the starch diet. Although the MDA concentration in plasma was significantly decreased in all fruit groups, urine MDA excretion rate was decreased only in the linseed oil/apple and linseed oil/apple/strawberry/tomato groups. Supplementation with fruit significantly decreased DNA damage, was more pronounced in the linseed oil/apple and linseed oil/apple/strawberry/tomato groups. Significantly lower glutathione peroxidase concentrations were observed only in the linseed oil/strawberry group. Conclusions Our findings support the hypothesis that supplementation with apples, strawberries, or tomatoes effectively decreases oxidative stress by decreasing MDA formation in the body and by protecting mononuclear blood cells against increased DNA damage. This effect was particularly pronounced in the group supplemented with a fruit mixture; among the single fruit supplements, the most beneficial effect was obtained with apples.

Nutritional Differences between Elderly Men and Women

Background: Many differences exist between the two genders, i.e., biological, sociological, and also behavioral, which often depend on age. Objectives: The aim of this study was to find characteristic differences between nutritional habits of elderly men and women. Methods: 266 elderly people (109 men >65 years, and 157 women >60 years) were consecutively selected from primary care patients, in Budapest, Hungary. A self-managed questionnaire was filled in on lifestyle and eating habits, including a food frequency questionnaire. Medical check-up, registration of anthropometrical parameters, and laboratory tests were also performed. 53 subjects were also involved in a 3-day dietary recall. Results: The meal frequency was increased during aging in both genders, especially in men. Lunch was preferred by most of the women as a principal meal, but one quarter of the men had a filling dinner instead of lunch. Alcoholic beverages were consumed more and frequently by men. The fluid intake was low, especially in women. Milk and diary products, fresh fruit, bread, biscuits, chocolate, coffee and vitamin supplements were consumed more frequently by women. Almost all types of meat, eggs, and vegetables were more preferred by men and their energy intake was also higher (9.75 vs. 8.78 MJ). In both genders, fat represented a higher ratio (39%) of energy intake than recommended. The increase of body weight from youth to elderly was greater in women (14.04 vs. 10.65 kg). Prices had a higher impact on food purchases by women. Conclusion: High energy intake over decades led to overweight in both genders (BMI >27 kg/m²). It seemed that the eating habits and food choices of women were closer to healthy ones and recent recommendations, although due to metabolic reasons and to the lower energy expenditure, they gained more weight. The elderly are the target population on every level of medical care. Further evaluations are needed to determine the differences in nutrition and to promote better administration of drugs and to establish public health suggestions.

Effects of L-carnitine, Selenium-enriched Yeast, Jujube Fruit and Hwangto (Red Clay) Supplementation on Performance and Carcass Measurements of Finishing Pigs

Fifty castrated crossbred (Landrace×Yorkshire) pigs, weighing an average of 60.6±3.1 kg were allotted to one of five treatments in a randomized block design to examine the effects of dietary inclusion of 0.1% L-carnitine (50 ppm carnitine), 0.1% selenium-enriched yeast (0.3 ppm selenium), 0.1% Jujube fruit or 0.1% Hwangto (Red clay) on pig performance and carcass quality. All diets were based on corn, wheat, soybean meal and wheat bran and were formulated to supply 13.8 MJ DE/kg. Dietary supplementation did not influence daily gain (p = 0.57), feed intake (p = 0.52), or feed conversion (p = 0.32). Digestibility of dry matter (p = 0.60), organic matter (p = 0.74), crude protein (p = 0.76), crude fibre (p = 0.70) and energy (p = 0.75) were also unaffected by inclusion of any of the additives. Tissue samples taken from the longissimus muscle showed that the levels of carnitine (p = 0.0001) and selenium (p = 0.0001) were significantly higher with dietary inclusion of carnitine or selenium-enriched yeast. Dietary treatment did not affect dressing percentage (p = 0.33), carcass lean yield (p = 0.99) or first, 10 th and last rib midline backfat depth (p = 0.45, 0.82 and 0.47, respectively). Dietary treatment also did not affect the percentages of tenderloin (p = 0.37), bacon (p = 0.36), fat and bone (p = 0.56), picnic shoulder (p = 0.25), skirt (p = 0.80), fresh ham (p = 0.31) or ribs (p = 0.79). However, pigs fed the diet containing Jujube fruit had a higher percentage of Boston butt than pigs fed the carnitine or selenium supplemented diets (p = 0.01). Pigs fed added Hwangto had a higher (p = 0.04) percentage of loin compared with pigs fed supplementary selenium or Jujube fruit. Loin muscle from pigs fed carnitine had a significantly lower Hunter colour value for L (whiteness, p = 0.004) and a higher value for a* (redness; p = 0.069). The overall results indicate that supplementation with L-carnitine and selenium-enriched yeast can produce pork containing higher levels of carnitine and selenium, which could provide health benefits for consumers of pork without detrimental effects on pig performance. (Asian-Aust. J. Anim. Sci. 2006. Vol 19, No. 2 : 217-223)

Associations between Breast Cancer Risk and the Catalase Genotype, Fruit and Vegetable Consumption, and Supplement Use

Observed weak or null associations between fruit and vegetable intake and breast cancer risk could be due to heterogeneity in endogenous antioxidant capabilities. The authors evaluated potential relations between a functional polymorphism in catalase, an antioxidant enzyme, and breast cancer risk, particularly in relation to fruit and vegetable intake and supplement use. Women (1,008 cases and 1,056 controls) in the Long Island Breast Cancer Study Project (1996-1997) were interviewed, completed a food frequency questionnaire, and provided blood for genotyping. The high-activity catalase CC genotype was associated with an overall 17% reduction in risk of breast cancer compared with having at least one variant T allele (odds ratio = 0.83, 95% confidence interval: 0.69, 1.00). Vegetable and, particularly, fruit consumption contributed to the decreased risk associated with the catalase CC genotype. Associations were more pronounced among women who did not use vitamin supplements, with a significant multiplicative interaction (p(interaction) = 0.02) for the CC genotype and high fruit intake (odds ratio = 0.59, 95% confidence interval: 0.38, 0.89), and there was no association among supplement users. These results indicate the importance of diet, rather than supplement use, in concert with endogenous antioxidant capabilities, in the reduction of breast cancer risk. CC genotypes were prevalent in approximately 64% of controls; thus, the preventive potential for fruit consumption has widespread implications.

Dietary phylloquinone intake as a potential marker for a heart-healthy dietary pattern in the Framingham Offspring cohort

Associations were evaluated among self-reported dietary intakes of phylloquinone (vitamin K-1), lifestyle characteristics, and intermediary markers of cardiovascular disease risk in a population-based cohort of men and women. Dietary phylloquinone intakes were assessed by food frequency questionnaire in 1,338 men and 1,603 women (mean age, 54 years) participating in the Framingham Heart Study. Cross-sectional associations with lifestyle characteristics and lipid profiles, including total cholesterol, low-density lipoprotein and high-density lipoprotein cholesterol, and triglyceride concentrations, were estimated across increasing quintile categories of phylloquinone intakes. Participants in the highest quintile category of phylloquinone intake consumed more fruit, vegetables, fish, dietary fiber, and dietary supplements ( P<.001), and consumed less meat and less saturated fat ( P<.001). Higher phylloquinone intakes were also associated with lower triglyceride concentrations ( P<.001). In conclusion, a high phylloquinone intake may be a marker for an overall heart-healthy dietary pattern.

Lutein, but not α-tocopherol, supplementation improves visual function in patients with age-related cataracts: a 2-y double-blind, placebo-controlled pilot study

Objective We investigated the effect of long-term antioxidant supplementation (lutein and α-tocopherol) on serum levels and visual performance in patients with cataracts. Methods Seventeen patients clinically diagnosed with age-related cataracts were randomized in a double-blind study involving dietary supplementation with lutein (15 mg; n = 5) , α-tocopherol (100 mg; n = 6), or placebo ( n = 6) , three times a week for up to 2 y. Serum carotenoid and tocopherol concentrations were determined with quality-controlled high-performance liquid chromatography, and visual performance (visual acuity and glare sensitivity) and biochemical and hematologic indexes were monitored every 3 mo throughout the study. Changes in these parameters were assessed by General Linear Model (GLM) repeated measures analysis. Results Serum concentrations of lutein and α-tocopherol increased with supplementation, although statistical significance was reached only in the lutein group. Visual performance (visual acuity and glare sensitivity) improved in the lutein group, whereas there was a trend toward the maintenance of and decrease in visual acuity with α-tocopherol and placebo supplementation, respectively. No significant side effects or changes in biochemical or hematologic profiles were observed in any of the subjects during the study. Conclusions Visual function in patients with age-related cataracts who received the lutein supplements improved, suggesting that a higher intake of lutein, through lutein-rich fruit and vegetables or supplements, may have beneficial effects on the visual performance of people with age-related cataracts.

Serum carotenoids and breast cancer.

The consumption of vegetables and fruit may protect against many types of cancer, but research evidence is not compelling for breast cancer. Carotenoids are pigments that are present in most plants and have known antioxidant properties. Blood concentrations of carotenoids have been proposed as integrated biochemical markers of vegetable, fruit, and synthetic supplements consumed. In a case-control study (270 cases, 270 controls) nested within a cohort in New York during 1985-1994, the carotenoids lutein, zeaxanthin, beta-cryptoxanthin, lycopene, alpha-carotene, and beta-carotene were measured in archived serum samples using liquid chromatography. There was an evident increase in the risk of breast cancer for decreasing beta-carotene, lutein, alpha-carotene, and beta-cryptoxanthin. The risk of breast cancer approximately doubled among subjects with blood levels of beta-carotene at the lowest quartile, as compared with those at the highest quartile (odds ratio = 2.21; 95% confidence interval (CI): 1.29, 3.79). The risk associated with the other carotenoids was similar, varying between 2.08 (95% CI: 1.11, 3.90) for lutein and 1.68 (95% CI: 0.99, 2.86) for beta-cryptoxanthin. The odds ratio for the lower quartile of total carotenoids was 2.31 (95% CI: 1.35, 3.96). These observations offer evidence that a low intake of carotenoids, through poor diet and/or lack of vitamin supplementation, may be associated with increased risk of breast cancer and may have public health relevance for people with markedly low intakes.

Immune Function in Elderly Smokers and Nonsmokers Improves During Supplementation with Fruit and Vegetable Extracts

Background: Epidemiological evidence suggests fruits and vegetables reduce the risk of cardiovascular disease (CVD) and cancer. Immune function declines with age as CVD and cancer incidence rises and may be related to poor antioxidant status. Objective: To investigate how fruit and vegetable extracts (Juice Plus™) containing multiple antioxidants and phytonutrients affect immune function in the elderly. Design: Subjects ( n = 53; aged 60–86 years, mean = 68 years) consumed extracts for 80 days and two blood samples were taken at baseline and then one at days 40 and 80. Results: Significant increases were found in the serum antioxidants when baseline values were compared with day 80; lutein/zeaxanthin ( p < .005), α-carotene ( p < .0001), β-carotene ( p < .0001), lycopene ( p < .05), and α-tocopherol ( p < .005). Spontaneous proliferation of PBM cells increased significantly ( p < .0001). Natural killer (NK) cell cytotoxicity significantly increased at effector to target cell ratios of 100:1 ( p < .0001), 50:1 ( p < .0005), and 25:1 ( p < .005). Supernatant from PBM cells stimulated with phytohemaglutinin (PHA; 10 μg/mL) resulted in significant twofold increases in interleukin-2 (IL-2) ( p < .0001). Additionally, statistically significant increases in IL-2 production were observed in smokers ( p < .005). Conclusions: Fruit and vegetable extract supplementation significantly enhanced multiple measures of immune function in elderly subjects, and improved IL-2 levels in smokers. Fruit and vegetable extract supplementation offers a novel way to improve compliance with current nutritional recommendations and may potentially lower disease risk.

Supplementation with fruit and vegetable extracts may decrease DNA damage in the peripheral lymphocytes of an elderly population

Fruit and vegetable consumption has been heralded for its ability to decrease the overall risk of developing cancer and other diseases. Mounting evidence supports the beneficial nature of antioxidants, carotenoids, and other phytonutrients found in fruits and vegetables. One proposed mechanism of antioxidant protection is the shielding of cellular DNA from oxidative damage and therefore mutations. This may be especially helpful in older populations. We tested the concept that a daily regimen of supplementation with fruit and vegetable extracts (JuicePlus™) would reduce the amount of DNA damage in the peripheral lymphocytes of the elderly. In a blind study, a group of twenty elderly volunteers (mean age=68) were given supplements twice daily for 80 days with blood samples drawn before and after intervention. These samples were compared using the comet assay, a technique that quantifies DNA damage to individual nuclei. Each sample was tested in triplicate, and tail moment data was collected from over 200 comets per sample. Paired t-test analysis revealed a highly significant (p<0.0001) decrease in measured DNA damage between pre (13.24±2.77) and post (4.41±2.76) treatment tail moment. Screening of test subjects' personal data showed no apparent relationship between age, sex, or smoking. In this initial study, we conclude that a daily course of fruit and vegetable extract supplementation may reduce the level of DNA damage found in the peripheral lymphocytes of seniors.

Total Student-Athlete Development (TSAD): A Partnership Between Health Services, Athletics, and the coordinated Program (CU) in Dietetics

Athletic trainers, coaches and registered dietitians have documented varied eating patterns in college athletes. At Eastern Michigan University, the basketball team's poor eating behavior and fatigue were noted by the coaching staff. Subsequently, a multi-disciplined group of professionals, including a psychologist, academic service center specialists, and dietitians formed TSAD and developed a program to address these issues. Informed consent was obtained for a serum anemia screening test. Also, CP dietetic students performed bioelectrical impedance analysis (BIA, RJL Systems) and 24 hour dietary recall to evaluate nutritional status. During this assessment, the CP students asked 4 questions designed to assess fluid/hydration knowledge. Dietary analysis (Diet Analysis Plus, ESHA Research) revealed inadequate intakes of milk/dairy, vegetable and bread/grains groups as compared to the Food Guide Pyramid. The 12 players exceeded the fats/sweets recommendations with an average 35 servings/player being consumed; however, meat intake guidelines were met. The majority of players drank fruit juices to meet the recommended fruit intake. BIA indicated 9 of 12 players were within desirable body weight and composition ranges. Three players were candidates for weight gain regimens. Fluid/hydration questions revealed 33% of the team did not know the important role hydration plays in performance. Academic study table and psychological support sessions were scheduled to enhance student learning and well-being. After this assessment, EMU coaches instituted fruit supplementation after all practice sessions and water breaks increased in frequency during practice. The coaching staff, subjectively, noted that players maintained peak performance through the basketball season.

Citrus fruit supplementation reduces lipoprotein oxidation in young men ingesting a diet high in saturated fat: presumptive evidence for an interaction between vitamins C and E in vivo

To determine the effects of vitamin C on cardiovascular risk factors, we studied dietary vitamin C enrichment in 36 healthy male students consuming a diet high in saturated fatty acids. After a 1-mo run-in period during which the subjects consumed approximately 50 mg ascorbic acid/d (low-C diet), half of the subjects were randomly assigned to receive 500 mg ascorbic acid/d for an additional 2 mo (high-C diet). Plasma ascorbic acid increased from 13.5 micromol/L with the low-C diet to 51.7 micromol/L with the high-C diet. Plasma cholesterol increased slightly with the high-C diet, but not above baseline concentrations. This increase was offset by an increase in the lag period of in vitro LDL oxidation, which correlated with plasma ascorbic acid concentrations (r = 0.735, P = 0.0012). Lipoprotein vitamin E concentrations were unchanged with the two diets. There were no effects on concentrations of fibrinogen or factor VII. The fact that ascorbic acid reduced the in vitro susceptibility of lipoproteins to oxidation provides presumptive evidence for an interaction between aqueous and lipophilic antioxidants (vitamins C and E ) in maintaining the integrity of LDL particles.

Breeding orange‐winged Amazon parrots in captivity

AbstractCaptive propagation of parrots for the companion bird trade provides a potential means of reducing the economic incentive to capture these birds from the wild. To test whether environmental and social manipulations might stimulate reproduction in captive, wild‐caught orange‐winged Amazon parrots (Amazona amazonica), we compared reproductive performance in pairs presented with nest boxes (controls) or nest boxes plus additional environmental and social manipulations (enriched group) consisting of misting, fruit supplementation, nest hole restriction, enlarged nest boxes, and pair separation/reunification. In the first breeding trial, in 1990, enriched pairs were more likely to lay eggs (six of seven enriched pairs vs. one of eight control pairs; P &lt; 0.09). When treatments were reversed the following years (e.g., 1990 controls were exposed to enriched conditions in 1991), reproductive performance was not different between the groups (four of seven enriched pairs laid eggs vs. five of seven control pairs), although three pairs in the 1991 enriched group laid eggs which had not laid eggs before. These results are consistent with the idea that while enriched environments may enhance the probability of a first episode of egg laying in captivity, once pairs have laid eggs in captivity, little stimulation beyond that provided by nest box presentation is required to reinitiate egg laying. The environmental and social manipulations of the enriched conditions could be useful in increasing reproductive performance of captive Amazon parrots for the companion bird trade. © 1995 Wiley‐Liss, Inc.

30-day oral toxicity study of l-selenomethionine in female long-tailed macaques ( Macaca fascicularis)

Twenty female long-tailed macaques received nasogastric intubation of 0–600 μg/kg-day l-selenomethionine for up to 30 consecutive days. Selenium ingestion was well tolerated at all dose levels until the second to third week of the study at which time two animals given 600 μg/kg-day died. One animal from the 300 μg/kg-day group was removed from study on Treatment Day 19 due to selenium-induced hypothermia. In some cases, administered doses were reduced at the 300 and 600 μg/kg-day levels such that the final time-weighted average doses were 0, 25, 62–117, 150, 188–203, and 300 μg/kg-day. Six animals at the 188 μg/kg-day level or greater required nonscheduled fruit and dietary supplementation to prevent their impending demise. As the dose and duration of exposure increased, the incidence of anorexia, gastrointestinal distress, mucocutaneous toxicity, and frequency of reduced body temperature also increased. A dose-dependent reduction in body weight was also observed. At the greater doses, disturbances in menstrual function were evident, and were accompanied by the absence of serum progesterone concentrations above 1.0 ng/ml, reduced luteal phase lengths, increased intermenstrual intervals, and lowered estrogen excretion. A maximum tolerated dose of 150 μg/kg-day l-selenomethionine for 30 days was identified based on mean body weight reduction, hypothermia, dermatitis, xerosis, cheilitis, disturbances in menstruation, and the necessity of dietary intervention to prevent death at doses of 188 μg/kg-day or greater.

Urine mutagenicity as an indicator of exposure to dietary mutagens formed during cooking of foods.

Studies were undertaken with individuals fed fried bacon meals to determine whether fruit or vegetables, ingested along with bacon, modified uptake and subsequent excretion of bacon mutagen(s). Urinary mutagenic activity was significant in those who had consumed bacon or mixed bacon/vegetable or bacon/fruit meals within the previous 2 to 3 hr period. Although urine activity varied by a factor of 4 among 15 subjects who consumed different meals, there was no evidence from this investigation that fruit or vegetables contributed to the inherent variability in total urinary mutagenic activity. However, some differences in excretion kinetics may be attributable to vegetable or fruit supplements in mixed meals.