Fruit Skin Research Articles

Reuseable Fe3O4@PEI-DTC-Au@Ag magnetic nanocomposites: A versatile and sensitive SERS substrate for food safety assessment

A novel recyclable SERS substrate with magnetic manipulation capability and surface-enhanced Raman scattering (SERS) effect based on Fe3O4@PEI-DTC-Au@Ag (FPDAA) core-shell nanocomposites has been successfully prepared by engineered hydrothermal deposition and seed deposition techniques. The uniformity and SERS efficacy of these substrates, incorporating various Au@Ag core-shell nanocomposites with differing Ag shell thicknesses, were evaluated using 4-aminothiophenol (4-ATP) molecules. The optimal SERS substrate, Fe3O4@PEI-DTC-Au@Ag-3 (FPDAA-3), exhibited an enhancement factor (EF) of up to 1.121×106. This remarkable performance can be attributed to the synergistic effect between the magnetic plasmon of the Fe3O4 nanoparticles (NPs) core and the surface plasmonic resonance properties of Au@Ag core-shell nanocomposites, which create abundant interparticle hotspots within the FPDAA nanocomposites. Furthermore, the FPDAA-3 nanocomposite was employed as a SERS substrate for rapid thiram detection, achieving a minimum detectable concentration of 1×10−9 M. Notably, the relative standard deviation of SERS measurements from 50 randomly selected points was found to be less than 12.13 %, and the SERS spectra intensity showed minimal variation after 5 cycling tests, underscoring the excellent uniformity and recyclability of the FPDAA-3 substrate. The highly reusable and reliable FPDAA-3 nanocomposites enable direct on-site detection of thiram pesticide residues on fruit skins, thereby offering significant implications for food safety assessment and spectroscopic identification.

Effects of Promalin on Fruit Growth and Cuticle Properties of ‘Pinova’ Apple

Promalin (Valent BioSciences, Libertyville, IL, USA) is a proprietary mixture of gibberellin A4 + 7 and 6-benzyladenine that is widely used in apple production to improve the fruit shape, size, and skin quality. Promalin typically increases fruit size and length. However, the increased growth likely increases the strain in the fruit skin, which may exacerbate microcracking of the cuticle and, consequently, russeting. This study aimed to monitor the growth-stimulating effect of Promalin in three different regions of fruits and investigate whether Promalin affects cuticular microcracking via effects on the deposition of cuticular components or via the accumulation of strain in the cuticle. Four Promalin sprays (20 mg⋅L−1) were applied to runoff; the first was applied at full bloom, and the remaining sprays were applied at approximately weekly intervals thereafter. Fruit surface areas and fruit surface area growth rates of the Promalin-treated fruits were higher than those of the untreated control fruits. Promalin increased the fruit length, but it had no effect on the fruit equatorial diameter. In Promalin-treated fruits, the base of each sepal extended, thickened, and became fleshy as early as 15 days after full bloom (DAFB). Allometric growth analyses revealed higher constant differential growth ratios of the pedicel and calyx length (before 36 DAFB) in Promalin-treated fruits than in control fruits. After 36 DAFB, the difference in constant differential growth ratios between Promalin-treated fruits and control fruits decreased. Cuticle mass per unit area increased with time in all regions of the fruit surface and was slightly (+3.3%) but significantly higher in fruits treated with Promalin than in control fruits. Additionally, the biaxial strain release was slightly and significantly lower in Promalin-treated fruits than in control fruits. When the isolated, cuticle was ablated from the inner surface and dewaxed, strain relaxation in the control fruits was higher than that in the Promalin-treated fruits. It was concluded that Promalin treatment increases the length of the fruit by increasing the lengths of the pedicel and calyx regions early during fruit development. Promalin only slightly increased cuticle deposition and fixation of cuticular strain. Promalin had no effects on microcracking or russeting.

Compost amendment in urban gardens: elemental and isotopic analysis of soils and vegetable tissues

Urban horticulture poses a sustainable form of food production, fosters community engagement and mitigates the impacts of climate change on cities. Yet, it can also be tied to health challenges related to soil contamination. This work builds on a previous study conducted on eleven urban gardens in the city of Vienna, Austria. Following the findings of elevated Pb levels in some soil and plant samples within that project, the present study investigates the elemental composition of soil and plants from two affected gardens 1 year after compost amendment. Inductively coupled plasma mass spectrometry (ICP-MS) analysis of skin, pulp and seeds of tomato fruits revealed minor variations in elemental composition which are unlikely to have an impact on food safety. In turn, a tendency of contaminant accumulation in root tips and leaves of radishes was found. Washing of lettuce led to a significant reduction in the contents of potentially toxic elements such as Be, Al, V, Ni, Ga and Tl, underscoring the significance of washing garden products before consumption. Furthermore, compost amendments led to promising results, with reduced Zn, Cd and Pb levels in radish bulbs. Pb isotope ratios in soil and spinach leaf samples taken in the previous study were assessed by multi-collector (MC-) ICP-MS to trace Pb uptake from soils into food. A direct linkage between the Pb isotopic signatures in soil and those in spinach leaves was observed, underscoring their effectiveness as tracers of Pb sources in the environment.Graphical

Isolasi dan Identifikasi Bakteri Asam Laktat dari Hasil Fermentasi Limbah Organik Kulit Buah (Eco-Enzyme)

The aim of this research is the isolation and identification based on the morphological characteristics of lactic acid bacteria in the fermentation of organic waste from fruit peels. Isolation of Lactic Acid Bacteria was carried out using the spread plate method on de Man, Rogosa, and Sharpe Agar (MRSA) media. The culture was incubated at 37 °C for 48 hours. The growing colonies were observed for morphological characteristics, and bacterial Gram staining was carried out. The results of the research showed that 10 bacterial isolates were obtained from the isolation of BAL from ecoenzymes on de Man, Ragosa, and Sharpe Agar (MRSA) media, namely E-BAL 01, E-BAL 02, E-BAL O3, E-BAL 04, E-BAL 05, E-BAL 06, E-BAL 07, E-BAL 08, E-BAL 09, and E-BAL 10, and 10 isolates were characterized as Gram-positive with a bacillary cell form. In conclusion, 10 isolates were found with characteristics that supported MRSA media, namely isolates of lactic acid bacteria of the genus Lactobacillus. This is confirmed by the results of biochemical tests, namely that it is immobile, does not produce the catalase enzyme, and does not have the ability to produce gas. Keywords: Lactic Acid Bacteria (LAB), Ecoenzymes, Fruit Skin

Utilization of Organic Waste as A Natural Detector of Formaldehyde on-Campus Snacks

The aim of this research is to reduce household organic waste through the use of organic waste as a natural detector of formaldehyde content in food snacks. The organic waste used in this study was red garlic skins, dragon fruit skins and purple sweet potato skins. This research is an experimental research. The stages of the research started with the extraction of anthocyanins from the three organic wastes, direct detection of formalin and detection of food ingredients. The formalin concentrations tested were 0.5%, 1%, 2%, 5% and 25%. Detection ability can be seen from the color change between control and formalin treatment. Garlic skin produces a red extract, dragon fruit skin produces an orange extract, and purple sweet potato skin produces a deep red extract. Test results on 20 samples of food snacks around the campus using the three organic waste extracts proved that all samples contained formaldehyde in varying concentrations. The highest 50% formalin content was found in yellow noodles and meatballs. The conclusion is that purple beetle skin, dragonfruit skin, and red garlic skin can be used as natural detectors for formalin in campus snacks.

First report of Bactrocera dorsalis (Hendel) (Diptera, Tephritidae) on the white - fleshed dragon fruit Selenicereus undatus (Haworth) D.R. Hunt (Cactaceae) in India

The oriental fruit fly Bactrocera dorsalis (Hendel) has been reported on the white fleshed dragon fruit Selenicereus undatus (Haworth) D.R.Hunt for the first time in India. Symptoms of infestation and the duration of all life stages were observed. Females laid eggs just beneath the mature fruit skin. The tissue surrounding the egg mass turned light yellow. Oviposition punctures were visible on the fruit rind and the feeding of the maggots led to decay of internal contents, foul smell and the fruit turned in to a discolored semi liquid mass. Full grown maggots exited the fruit and entered a period of inactivity before pupation in moist soil. The egg, maggot and pupal stages lasted for 1.6± 0.40, 8.0 ± 0.40 and 8.0 ± 0.31 days, respectively under laboratory conditions. Adult longevity was 9.6 ± 0.5 days in the case of females and 6.4 ± 0.50 days for males.

Flame-catalytic infrared dry system for tomato continuous peeling

To eliminate the need for alkali and excess water while accelerating the industrial application of dry tomato peeling, a highly efficient industrial flame-catalytic infrared continuous peeling apparatus was designed. The synthesis of the catalytic layer (Pt/Al2O3) within the device was optimized to improve cost-effectiveness and enable modular installation, making it suitable for industrial applications. This study determined the optimal production parameters for industrial flame-catalytic infrared peeling (F-CIP) through experiments. Current findings demonstrated that, under optimal F-CIP conditions, characterized by a flame heating time of 5.8 s, an infrared heating temperature of 456 °C, an infrared heating distance of 70.6 mm, and an infrared heating duration of 246 s, peeling efficiency reached 99.2±0.9 %, with a peel loss of 4.1±0.3 %. Comparative analysis with lye peeling (LP) and hot water peeling (HWP) showed that F-CIP reduced hardness and peel loss and better preserved nutritional and antioxidant compounds. The mechanism responsible for the loosening and cracking of the fruit skin in the F-CIP peeling process involved a reduction in adhesion between the skin and the pulp due to the alteration of the pectin structure, an expansion of cell structure due to internal vaporization, and an increase in the Young’s modulus of the tomato skin. The F-CIP method, with its proven peeling efficiency, energy-saving attributes, and environmentally friendly features, is expected to attract increased attention from equipment manufacturers and the tomato industry. This paper serves as a foundational guide and contributes to the application and parameter control of F-CIP in the tomato peeling.

Karakterisasi Morfologi Sukun (Artocarpus altilis Forb.) Di Desa Ohoitahit Dan Danar Kepulauan Kei Kecil Kabupaten Maluku Tenggara

This study aims to characterize the morphology of breadfruit (Artocarpus Altilis Forb.) In Ohoitahit Village and Danar Kecil Kecil Kecil Southeast Maluku. The sampling method through two sample units, namely key informants and target plant accessions. Key informants are community leaders who have in -depth knowledge about the genetic diversity of breadfruit in the village that is used as a sample. Target plant accessions are a variety of varieties of identification of key informants. Withdrawal of samples for critical informants is carried out by snowball sampling methods, while target plant accessions are selected using accidental sampling methods (accidental sampling) or intentional sampling (purposive sampling). Data presentation using descriptive methods. The results showed a significant morphological diversity in breadfruit in the Kei Islands. There is a diversity of morphology in breadfruit based on 31 observation variables. In the character of the tree, there is diversity, among others, the tree's shape, the trunk's surface, the branching pattern, and the direction of growing. In the leaf character there is diversity, namely the color of the leaf, the tip of the leaf, the length of the leaf strands, and the width of the leaf strands, and on the tip of the leaf. Flower characters have a diversity that is, the color of male flowers, male flower positions, and female flower positions. Fruit character has diversity is the shape of the fruit, the texture of the fruit skin, the sap on the skin, the skin color of the fruit, the length of the fruit, the weight of the fruit, and the fruit yield.

HASIL PEMERIKSAAN TELUR SOIL TRANSMITTED HELMINTH MENGGUNAKAN CAMPURAN PEWARNA ALAMI DARI DAUN MIANA DAN KULIT BUAH NAGA

The simplest method for checking worm eggs is by a native technique using a 2% eosin reagent. Eosin itself has properties that are not easily decomposed, causing hazardous and easily flammable waste. Hence, an alternative staining method is needed, environmentally friendly, using natural materials, such as natural dye substances. This research aims to understand the depiction of results in examining Soil Transmitted Helminth (STH) eggs using mint leaves and dragon fruit skin as alternative reagents. The research method involves examining feces using a direct method (wet preparation) to detect worm eggs in feces directly using a 2% Eosin solution. The comparison results between dragon fruit peel extract concentration using aquadest, tested with SPSS analysis version 20 using the Kruskal-Wallis method with a significance value of 0.09 ≥ 0.05, indicating significantly different research outcomes. Thus, it can be concluded that there is no difference in staining quality compared to the control. The conclusion drawn from this study is that there's no difference in staining worm egg suspensions using mint leaf extract. This is evident as when observing the worm egg suspension using mint leaf extract, the worm eggs were not clearer, unlike with dragon fruit skin and miana.

The Impact of Modified Atmosphere Storage Treatment on Glucose Levels and Mass Transfer Coefficients: A Study Based on Fruit Skin Thickness

The Impact of Modified Atmosphere Storage Treatment on Glucose Levels and Mass Transfer Coefficients: A Study Based on Fruit Skin Thickness

Dragon Fruit Skin Tea with Added Salak Skin as Tanin and Pandan Leaves as Fragrance

Dragon Fruit Skin Tea with Added Salak Skin as Tanin and Pandan Leaves as Fragrance

R2R3-MYB transcription factor CsMYB60 controls mature fruit skin color by regulating flavonoid accumulation in cucumber.

Skin color is an important trait that determines the cosmetic appearance and quality of fruits. In cucumber, the skin color ranges from white to brown in mature fruits. However, the genetic basis for this important trait remains unclear. We conducted a genome-wide association study of natural cucumber populations, along with map-based cloning techniques, on an F2 population resulting from a cross between Pepino (with yellow-brown fruit skin) and Zaoer-N (with creamy fruit skin). We identified CsMYB60 as a candidate gene responsible for skin coloration in mature cucumber fruits. In cucumber accessions with white to pale yellow skin color, a premature stop mutation (C to T) was found in the second exon region of CsMYB60, whereas light yellow cucumber accessions exhibited splicing premature termination caused by an intronic mutator-like element insertion in CsMYB60. Transgenic CsMYB60c cucumber plants displayed a yellow-brown skin color by promoting accumulation of flavonoids, especially hyperoside, a yellow-colored flavonol. CsMYB60c encodes a nuclear protein that primarily acts as a transcriptional activator through its C-terminal activation motif. RNA sequencing and DNA affinity purification sequencing assays revealed that CsMYB60c promotes skin coloration by directly binding to the YYTACCTAMYT motif in the promoter regions of flavonoid biosynthetic genes, including CsF3'H, which encodes flavonoid 3'-hydroxylase. The findings of our study not only offer insight into the function of CsMYB60 as dominantly controlling fruit coloration, but also highlight that intronic DNA mutations can have a similar phenotypic impact as exonic mutations, which may be valuable in future cucumber breeding programs.

Mechanisms governing melon fruit skin pigmentation: Insights from transcriptome sequencing and whole-genome bisulfite sequencing analyses

Fruit skin color is a critical agronomic trait determining fruit quality, but the mechanisms governing melon (Cucumis melo L.) fruit skin pigmentation remain inadequately characterized. We performed cytological, transcriptomic, and whole-genome bisulfite sequencing (WGBS) comparative analyses on two near-isogenic lines (NILs) at different developmental stages: the green-skinned (GS) and white-skinned (WS) melon. The enriched functions among the 536 identified differentially expressed genes (DEGs) were related to chloroplast development, pigment metabolism, and photosynthetic pathway. The genes in these pathways were downregulated in the WS line, potentially contributing to the decreased chlorophyll content and abnormal chloroplast development in WS. WGBS analysis revealed that the levels of genomic DNA methylation in WS progressively exceeded those in GS as the fruits developed. The integrated analysis of the transcriptome and methylome identified DMR-associated DEGs (CHLI, CRD1, PORA, and HCAR) involved in chlorophyll metabolism. Notably, PORA exhibited downregulated expression and was hypermethylated in WS fruits during two developmental stages, showing a coordinated expression with the chlorophyll contents in developing WS and GS fruits. Furthermore, we identified a transcription factor gene, MELO3C011576, that was closely associated with the expression of PORA and chlorophyll levels across three developmental stages in both NILs. We speculate that PORA positively regulates chlorophyll biosynthesis in the two NILs, with its expression may be co-controlled by DNA methylation and transcription factors.

FORMULASI DAN EVALUASI SEDIAAN SERUM WAJAH EKSTRAK ETANOL KOMBINASI DARI EKSTRAK KULIT JERUK NIPIS (Citrus aurantiifolia) DAN EKSTRAK KULIT BUAH NAGA MERAH (Hylocereus polyrhizus)

Background: The skin of the key orange (Citrus aurantiifolia) contains kuersetin, miristin, routinely, tangerine, naringin and hesperine, while the skin of the red dragon fruit (Hylocereus polyrhizus) contains betaalanin, anthocyanin, vitamin a, vitamin a, vitamin e, turnioid, flavonoid, tiamin, niacin, piridoine, kobalamin, carotene, and fitoalbumin. The research was intended to formulate it in a availability of facial serum and then evaluate its quality formula for a better facial serum from 16-4954-1998. Methods : This method of research is experimental in the laboratory by making 4 formulas consisting of F1 as serum bases, F2 ratio EKJN and EKBNM (1:1), F3 ratio EKJN and EKBNM (1:2) ,F4 ratio EKJN and EKBNM (2:1).Results : The results of all the formulas for existing existing face-serum EKJN ethanol and EKBNM viscers show a clear, clear, yellowish color, a gel-based, characteristic and homogenous odor. pH and viscosity scores on each condensed formula. No irritation test on the formula showed irritation to the skin.Conclusion : Based on this, it can be concluded that the combined ethanol extract of lime peel extract and red dragon fruit peel extract can be formulated into a facial serum cosmetic preparation and meets the quality requirements according to SNI.

TESTING FOR ANTIBACTERIAL ACTIVITY OF FRUIT SKIN ETHANOL EXTRACT DUKU (Lansium domesticum Corr) AGAINST BACTERIA Salmonella Typhi

Salmonella typhimurium bacteria is the cause of typhoid fever. One of the herbal plants that can be used as an antibacterial drug is duku fruit peel (Lansium domesticum Corr). Phytochemical tests were carried out on duku fruit peel extract which was soaked in 96% ethanol solvent. The aim of this test is to identify secondary metabolites found in duku fruit skin. There are alkaloids, flavonoids, saponins, tannins and triterpenoids in the ethanol extract of duku fruit peel. This study involved four treatment groups with extract concentrations of 25 percent, 50 percent, and 75 percent, along with a positive control (chloramphenicol) and a negative control (distilled water). With inhibition zone diameters of 10.22 mm, 11.94 mm, and 13 mm, the ethanol extract of duku fruit peel showed antibacterial activity against Salmonella enterica bacteria. The results of univariate data analysis showed that the ethanol extract of duku fruit peel showed antibacterial activity at 25%, 50% and 75%. The results of univariate data analysis showed that the ethanol extract of duku fruit peel showed antibacterial activity against Salmonella typhi bacteria. In addition, the bacterial concentration increases with the width of the inhibition zone.

Application of gaussian filter and extraction features for quality control of fruit raw materials in the puree industry

The purpose of research in using Android-based raw material quality control applications in fruit puree factories is to enhance the industry's precision, uniformity, and efficiency. This application is developed to implement digital image processing utilizing two distinct methods: Gaussian filters and feature extraction. The implemented application captures guava data using the camera of an Android phone and subsequently resizes the image to dimensions of 600x600 pixels. Subsequently, the image colors are recovered by employing a Gaussian filter that operates on normalized Red-Green-Blue values. A minimum of 120 photos of red guava fruit from the raw material of one of the puree companies were subjected to image processing procedures. The application solely focuses on the color and texture of the fruit's skin, ensuring that the sample remains undamaged while adhering to hygienic guidelines. The ripeness degree of guava fruit is determined by employing an image classification algorithm with the K-nearest neighbor method. The application validation using K-fold cross-validation achieved an accuracy of 90.0% and a precision of 90.27% when applied to color imagery. When feature extraction was used, the accuracy was 83.3%, with a precision of 83.4%. Color extraction provides a more precise method for identifying ripe guava. The utilization of guava fruit ripeness detection in the quality control of raw materials for the puree sector has been simplified and made more user-friendly through the development of an Android-based application. Officers are not obligated to possess specialized expertise regarding the quality of raw materials.

Varied Attributes of Jalapeño Pepper Cultivars Influence Fresh-cut Product Quality

During the past 40 years, the US fresh-cut product market has experienced a consistent increase in demand because consumers prioritize health and convenience. Increased interest in fresh-cut products and ready-to-eat vegetables has led to innovations in breeding, product selection, and packaging. However, despite the increased popularity of bell pepper and chile pepper (Capsicum annuum L.), research of fresh-cut jalapeño pepper is limited. This study was conducted to identify jalapeño cultivars that could be suitable as a raw fresh-cut product and explore measures beyond tissue membrane electrolyte leakage (EL) of processed products that may be useful for the identification of cultivars suitable for fresh-cut applications. A total of 22 fresh-cut parameters were examined across five cultivars of jalapeño peppers and 10 intercrosses of these cultivars, including visual quality based on an image analysis via a computer vision system, package headspace gas composition, tissue membrane EL, and texture. Based on our results, the genotypes were grouped into five clusters using a cluster analysis. Variables including tissue softening (r2 = 0.95), EL (r2 = 0.95), total energy of the mesocarp (r2 = 0.95), and package headspace carbon dioxide (CO2) partial pressure (r2 = 0.94) had strong associations with the cluster. A principal component analysis with biplots further confirmed the results. Cultivars Goliath and Emerald Fire and their hybrids in the first and second clusters showed good quality for fresh-cut applications. The fifth cluster, represented by a single cultivar, Jalapeño M, had the smallest physical size, rapid shelf-life decline, accumulated CO2 partial pressures, increased EL, and rapid tissue softening in comparison with the other genotypes. All jalapeño cultivars except Jalapeño M maintained good quality until day 14 postprocessing, and some maintained good quality until 21 days postprocessing. Hybrid crosses suggested that two of the cultivars evaluated, Goliath and Emerald Fire, were useful as parents when transferring superior fresh-cut quality traits to progeny. Traditionally, the EL level has been used as an index of freshness (or tissue deterioration). Our results showed that other quality analyses, including measurements of tissue softening via an imaging analysis, and physical analyses of tissue firmness can also be used as indices for the freshness of fresh-cut jalapeños. The results suggest that fruit size, wall thickness, and skin toughness might be useful as predictive measures in the field for the selection of jalapeño genotypes with superior fresh-cut quality.

KAJIAN FORTIFIKASI DAGING DAN KULIT BUAH NAGA MERAH (Hylocereus polyrhizus) TERHADAP MUTU MIKROBIOLOGI DAN ORGANOLEPTIK TAPAI KETAN PUTIH

White glutinous tapai is a fermented food product in which yeast is added in manufacturing. Red dragon fruit can be added to increase the nutritional value, taste and product diversification of processed white sticky rice tapai. Besides being rich in nutritional value, red dragon fruit also contains antioxidant compounds, natural food fiber and natural colouring compounds that display a purplish-red colour. This study aims to determine the effect of fortification of red dragon fruit meat and skin on fiber content and organoleptic quality of white sticky rice. This study was experimental, using a completely randomized design (CRD), with the fortification of red dragon fruit meat and skin in the production of white sticky rice tapai. The type of treatment: T1 = white glutinous rice without red dragon fruit (control), T2 = white glutinous rice + red dragon fruit pulp, T3 = white glutinous rice + (flesh and skin) of red dragon fruit, and T4 = white glutinous rice + red dragon fruit skin). Observational data were analyzed by analysis of variance (ANOVA) at a 5% significance level and follow-up test with HSD at the same significant level. The results of the analysis of the quantity of microorganisms in white sticky rice tapai yeast with fortification treatment of red dragon fruit flesh and skin showed that the sticky rice tapai contained 2.9x104 microbes in T1, 6.0x104 in T2, 7.1x104 in T3 and 4.0x104 in T4 and research results organoleptic properties of the colour, aroma, taste and texture score parameters. The best treatment preferred by the panellists was the T2 treatment (fortification of red dragon fruit pulp) with a fiber content of 4.13%, a pH value of 5.13, an aroma value score of 3.39 (slightly tapai flavour), a taste of 4.11 (liked it), texture 3.29 (slightly soft) with a purplish red appearance 3.86. The use of a mixture of dragon fruit, dragon fruit skin is able to produce the most suitable organoleptic assessment for white sticky rice compared to the use of dragon fruit skin from the results of organoleptic panelists and statistical tests. The use of dragon fruit in producing aroma, taste, and homogeneity are more preferred, while other parameters of color tend not to be affected. Total microbes, log 4.85, were the highest produced by fortification using dragon fruit skin. The use of dragon fruit flesh, which causes the sour taste is not strong and good color other treatments to be more acceptable to panelists.

The use of alternative coloring variations to mitotic observations of shallots using the squash method

Practicum cannot be carried out due to limited facilities and infrastructure such as dyes for observation of mitotic cell chromosomes in onions. Alternative dyes that can be obtained from nature can be explored. The purpose of this study was to obtain preparation of the mitotic division process in various onion root cells using alternative dyes using dyes from dragon fruit peel and turi flower. In obtaining the right preparation using the squash method. Data were obtained using observation sheets that observed each variation of the material. The instrument used in this study is a valid observation sheet. The results showed that the coloring of the preparation using dragon fruit skin and turi flowers was successful and clear coloring results were obtained. Coloring in practicum activities using turi flowers is better than dragon fruit coloring. Natural dyes from dragon fruit peel and turi flower can be used as alternative dyes in the squash method to observe onion root mitosis, replacing synthetic dyes.

PENGARUH KONSENTRASI DAN LAMA PERENDAMAN ATONIK TERHADAP PERKECAMBAHAN BENIH ROTAN JERNANG (Daemonorops draco Blume.)

Jernang rattan is produced from a palm species surface layer of fruit by resine with dark red. Jernang rattan cultivation has several obstacles due to limiteation of seed availability, low seed production, and has technical problems in the form of hard fruit skin. This study aimed to studied the effect of the interaction of atonic concentration and soaking time for jernang rattan seeds on their germination. There were two factors in this study: Atonic concentration treatment (5 levels) and soaking time (3 levels). The results show that increasing the germination of Jernang rattan seeds can be done using an atonic concentration of 15 ppm and soaking for 1 hour. This is because it provides better results on research variables.