Changes In Fruit Quality Research Articles

Fungicides and strawberry pollination-Effects on floral scent, pollen attributes and bumblebee behavior.

Fungicides are used in agriculture to protect crops from various fungal diseases. However, they may modulate the plants metabolism. Moreover, fungicides can accumulate in the environment and may cause toxic effects on non-target organisms such as nectar microbes and pollinators. Nectar microbes contribute to the volatile profile of flowers and can influence pollinators behaviour. Thus, fungicide treatment could potentially affect the pollination. In this study, we investigated the influence of fungicide treatment on floral attributes as well as the behavioural impact on bumblebees. In separate experiments, we used one or both strawberry cultivars (Fragaria × ananassa var. Darselect and Malwina), which were either kept untreated (control) or treated with either Cuprozin® progress or SWITCH® fungicide. We analysed various flower traits including volatiles, pollen weight, pollen protein, and the attraction of bumblebees towards the flowers in the greenhouse. Additionally, we analysed the viability of pollen and pollen live-to-dead ratio, as well as the composition of nectar fungi in the field. A treatment with Cuprozin® progress led to a lower emission of floral volatiles and a slightly lower pollen protein content. This had no impact on the visit latency of bumblebees but on the overall visit frequency of these flowers. The treatment with the fungicide SWITCH® resulted in a higher emission of floral volatiles as well as a delayed first visit by bumblebees. Furthermore, flowers of control plants were visited more often than those treated with the two fungicides. Plant-pollinator interactions are highly complex, with many contributing factors. Fungicides can have an impact on the pollen quality and pollinator attraction, potentially leading to an altered pollen dispersal by pollinators and a change in fruit quality.

Simulated extreme climate event alters a plant-frugivore mutualism

Anthropogenic climate change is contributing to an increase in the frequency of extreme weather events. These extreme events may affect interactions in mutualisms that provide key ecosystem functions, especially when the event is rare for a given system and participants are differentially affected. The tropical hardwood hammocks of Key Largo, Florida, USA are inhabited by a highly specialized endangered rodent, the Key Largo woodrat (Neotoma floridana smalli), which may be an important seed disperser of many native fleshy-fruited plant species. Other potential mammalian dispersers are generalist omnivores, northern raccoons (Procyon lotor) and Virginia opossums (Didelphis virginiana), that are less selective. We sought to demonstrate that extreme climate events could alter seed dispersal mutualisms by differentially affecting fruit preference of potential dispersers. We designed a replicated cafeteria-style experiment across the entire range of the Key Largo woodrat with fruit from sixteen native plant species and simulated an extreme climate event by exposing half of the fruits to sub-freezing temperatures. Freezing temperatures are rare in this tropical environment, but increased frequencies of these types of extreme events are predicted with climate change. Using camera traps, we monitored the removal of fruit and seeds by woodrats and the generalist consumers, predicting that changes in fruit quality resulting from exposure to sub-freezing temperatures would reduce preference by the more specialized woodrat relative to its generalist consumers. Indeed, exposure to subfreezing temperatures decreased the probability of fruit and seed removal by woodrats while generalist consumers preferentially removed more of the fruits and seeds exposed to sub-freezing temperatures. These data provide evidence that extreme climate events may affect species preferences for food asymmetrically, which may shift the dynamics of seed dispersal mutualisms. Over time, increasing frequencies of extreme weather events could indirectly affect communities and ecosystem services by shifting interactions between organisms.

Chilling-related browning of ‘Wonhwang’ pear cortex is associated with the alteration of minerals and metabolism

‘Wonhwang’ pear (Pyrus pyrifolia Nakai) is affected by a wide range of physiological disorders, especially internal browning (cortex browning) during long-term cold storage and subsequent shelf life. Postharvest fruit quality and metabolic changes of many Asian pears (P. pyrifolia) are well characterized, but little is known about the minerals and metabolites associated with internal browning induction and development in ‘Wonhwang’ pear during cold storage. The present study compares minerals and metabolites in cortex tissue of healthy fruit as well as symptomatic (browned) and sound tissues from fruit with internal browning that developed during long-term cold storage. P, K, Ca, sorbitol, shikimic acid, palmitic acid (C16:0), linoleic acid (C18:2), α-linolenic acid (C18:3), methionine, 1-hexenol, and (Z)-3-hexenal were lower in browned tissue than in healthy and sound tissues. In contrast, levels of glucose, fructose, fumaric acid, valine, leucine, isoleucine, lysine, phenolic compounds, lignoceric acid (C24:0), ethyl esters, and some volatile aldehydes were highest in browned tissue, compared with the other tissues. Many ethyl esters, aromatic amino acids, branched-chain amino acids, phenolic compounds, soluble solids content, fructose, and glucose were associated with internal browning. Furthermore, “tryptophan metabolism”, “arginine biosynthesis”, “alanine, aspartate, and glutamate metabolism”, “α-linolenic acid metabolism”, and “glycine, serine, and threonine metabolism” were the processes most impacted by internal browning. Overall, the largest metabolic differences appeared between damaged and asymptomatic cortex as several metabolic processes were influenced by internal browning.

The GRAS Salts of Na2SiO3 and EDTA-Na2 Control Citrus Postharvest Pathogens by Disrupting the Cell Membrane.

Sodium silicate (Na2SiO3) and ethylenediaminetetraacetic acid disodium salt (EDTA-Na2) are inorganic salts classified as 'Generally Recognized as Safe' (GRAS) compounds with great advantages in controlling various pathogens of postharvest fruits and vegetables. Here, we determined the median effective concentration (EC50) of Na2SiO3 (0.06%, 0.05%, 0.07% and 0.08%) and EDTA-Na2 (0.11%, 0.08%, 0.5%, and 0.07%) against common pathogens affecting postharvest citrus fruit, including Penicillium digitatum, Penicillium italicum, Geotrichum citri-aurantii, and Colletotrichum gloeosporioides. Na2SiO3 and EDTA-Na2 treatments at the EC50 decreased the spore germination rate, visibly disrupted the spore cell membrane integrity, and significantly increased the lipid droplets (LDs) of the four postharvest pathogens. Moreover, both treatments at EC50 significantly reduced the disease incidence of P. italicum (by 60% and 93.335, respectively) and G. citri-aurantii (by 50% and 76.67%, respectively) relative to the control. Furthermore, Na2SiO3 and EDTA-Na2 treatment resulted in dramatically lower disease severity of the four pathogens, while also demonstrating no significant change in citrus fruit quality compared with the control. Therefore, Na2SiO3 and EDTA-Na2 present a promising approach to control the postharvest diseases of citrus fruit.

Tomato puree quality from field to can: Effects of water and nitrogen-saving strategies

Processing tomato is a main source of phytomicronutrients in the human diet due to its micronutrient content, and the diversity of derived products consumed worldwide. A large proportion of processing tomatoes are grown under Mediterranean climate where increasing water scarcity and heat are expected to reduce yields and to have negative impacts on fruit quality. Despite this, the effects of changes in fresh fruit quality on processing and end-product quality have been under-investigated. It therefore seems imperative to work along the food chain to improve food sustainability while maintaining quality and access to important phytonutrients. The present study adopts a holistic approach from field to processing plant, assessing the effects of reduced water and nitrogen supplies on yields, fresh fruit quality and suitability for processing, and puree quality. The 5-year trial included eight varieties, several levels of water and nitrogen supply, and two processes. It was found that water deficit induced a decrease in fresh commercial yield, partially compensated by an increase in dry matter content. The yield loss was the result of a lower number of fruits per plant, while the average fruit weight and biochemical composition showed little change. Water use efficiency decreased above 120% of water demand restoration and improved under low nitrogen supply. Puree viscosity and dry matter content increased with water deficit with beneficial effects for the industry, while the reduction of nitrogen supply did not affect puree quality. This work highlighted the possibility of monitoring quality traits in tomato puree directly from field. Nevertheless, the trade-offs between WUE, quality and yield need to be optimized. Selection of varieties rich in lycopene could be a promising avenue as they appear more tolerant to water deficit. This work also stressed the need to reassess the crop coefficient for new varieties, to improve evaluation of water needs.

Study of Mineral Composition and Quality of Fruit Using Vascular Restrictions in Branches of Sweet Cherry.

Calcium (Ca) and carbohydrate (CHO) supply in sweet cherry have been associated with fruit quality at harvest and during storage. There is little published information integrating CHO and Ca availability and distribution in sweet cherry and their effects on fruit quality. Accordingly, in the 2019-20 season, vascular restrictions were imposed on the phloem (girdling, G, stopping phloem flow) and xylem (transverse incision, S, cutting 50% of xylem cross-section area) of individual vertical branches of the sweet cherry combination 'Lapins'/Colt trained as Kym Green Bush system to modify mineral and CHO composition in fruit and associate such changes with quality at harvest and storage. The girdling to the phloem was used to induce changes in CHO distribution. The transverse incision to the xylem was a tool to modify Ca distribution. Five treatments (TR) were implemented: TR1-CTL = Control (without vascular restriction), TR2-G, at its base, TR3-G + G: at its base, and G further up at the change of year between the second and the third years of growth TR4--S and TR5-S + G. The vegetative (i.e., shoot and leaf growth), reproductive (i.e., fruit set and yield) development and stomatal conductance were monitored. Each branch was divided into the upper (1-and 2-year-old wood) and the lower (3-and 4-year-old wood) segments of the restriction applied. The quality and mineral composition (Ca, Mg, K, and N) of fruit borne on each segment were measured at harvest. The upper segment of TR3-G + G branches were harvested 10 d before the lower segment. The fruit from the upper segment of TR3-G + G was the largest, the sweetest, and had the higher titratable acidity concentration. However, fruits of this segment were the softest, had the lowest Ca concentrations, and had the highest ratios of N:Ca and K:Ca, compared with the other TRs. TR3-G + G branches developed the highest number of lateral current season shoots including shoots below the second girdling in the lower segment of the branch. This vegetative flow of growth would explain the mineral unbalance produced in the fruit from the upper segment of the branch. TR2-G did not register changes in fruit quality and mineral concentration compared with TR1-CTL. Surprisingly, the fruit from the branches with xylem restriction did not show changes in Ca concentration, suggesting that the xylem stream was enough to supply the fruit in branches without lateral shoot development. Fruit firmness was positively related to fruit Ca concentration and negatively related to the ratios of K:Ca and N:Ca.

Changes in quality and microbiome composition of strawberry fruits following postharvest application of Debaryomyces hansenii, a yeast biocontrol agent

Microbial antagonists provide a new perspective on biological control by altering microbial diversity to control postharvest diseases of fruit. In this study, the biological control effect of Debaryomyces hansenii on postharvest diseases of strawberry and the influence on strawberry quality were determined. At the same time, the effect of D. hansenii on the composition of surface microbiome of strawberry fruit was analyzed by high-throughput sequencing technology. The results showed that D. hansenii treatment can inhibit the natural decay rate of strawberries, and the treatment group maintained a higher ascorbic acid content than the control group. However, there was no difference between the control and treatment groups in firmness, weight loss rate, soluble solids, titratable acidity, and other quality indicators. On the surface of strawberries treated with D. hansenii, the diversity of fungal community was changed, the composition and structure of the community were also altered (except the abundance of Debaryomyces spp.). Strawberries treated with D. hansenii could alter bacterial and fungal populations, inhibit plant pathogens, and lower the frequency of postharvest diseases in strawberries. This study helps to understand the microbial community structure on the surface of strawberries and guides the synthesis of strawberry postharvest disease complex biocontrol strains to achieve stable and sustainable postharvest disease control strategies of the fruits.

Changes in Growth, Fruit Quality, and Leaf Characteristics of Apple Tree (Malus domestica Borkh. ‘Fuji’) Grown under Elevated CO2 and Temperature Conditions

Changes in Growth, Fruit Quality, and Leaf Characteristics of Apple Tree (Malus domestica Borkh. ‘Fuji’) Grown under Elevated CO2 and Temperature Conditions

Integrative Analysis of Fruit Quality and Anthocyanin Accumulation of Plum cv. 'Cuihongli' (Prunus salicina Lindl.) and Its Bud Mutation.

Fruit color is one of the quality indicators to judge the freshness of a plum. The coloring process of plum skin is valuable for research due to the high nutritional quality of anthocyanins found in plums. 'Cuihongli' (CHL) and its precocious mutant variety 'Cuihongli Red' (CHR) were used to analyze the changes of fruit quality and anthocyanin biosynthesis during plum development. The results showed that, during the development of the two plums, the total soluble solid and soluble sugar contents were highest at the mature stage, as the titratable acid trended gradually downward as the fruits of the two cultivars matured, and the CHR fruit showed higher sugar content and lower acid content. In addition, the skin of CHR turned red in color earlier than CHL. Compared with CHL, the skin of CHR had higher anthocyanin concentrations, higher activities of phenylalanine ammonia-lyase (PAL), chalcone isomerase (CHI), dihydroflavonol-4-reductase (DFR), and UDPglucose: flavonoid-3-O-glucosyltransferase (UFGT), and higher transcript levels of genes associated with anthocyanin production. In the flesh of the two cultivars, no anthocyanin content was detected. Taken together, these results suggest that the mutation exerted a major effect on anthocyanin accumulation via modification of the level of transcription; thus, CHR advances the ripening period of 'Cuihongli' plum and improves the fruit quality.

Comparison of Fruit Color and Quality Changes During Fruit Development in ‘Kinnow’ and ‘W. Murcott’ Mandarins

Comparison of Fruit Color and Quality Changes During Fruit Development in ‘Kinnow’ and ‘W. Murcott’ Mandarins

Quantitative responses of tomato yield, fruit quality and water use efficiency to soil salinity under different water regimes in Northwest China

Reduced irrigation and high soil salinity are widely recognized to decrease tomato yield, but they can improve fruit quality and water use efficiency (WUE) when imposed appropriately. However, the response of plant to their interaction is still unclear, and the quantitative relationship of WUE and fruit quality with salinity under different water regimes is also lacking. This study investigated the responses of tomatoes in terms of yield, fruit quality, and WUE based on yield (WUEy), biomass (WUEb), and leaf (iWUE) to twelve treatments, consisting of combinations of three water regimes relative to the field capacity (FC) (W0, W1, and W2 refer to 95 % FC, 75 % FC, and 60 % FC, respectively) and four soil salinity levels (0 g, 2 g, 4 g, and 6 g salt mixtures added to 1000 g air-dried soil, respectively). Results showed that WUEy and WUEb had a negative correlation with salinity under different water regimes. Salinity resulted in a greater reduction in yield and biomass than water consumption compared to the control treatment. Therefore, lower water consumption of plants under W2 treatment alleviated the decrease of WUEy induced by salinity to a certain extent. From the leaf scale, iWUE was positively and linearly related with salinity, indicating that stomatal regulation was more prone to reduced stomatal conductance than the net photosynthetic rate under W0 and W1. Whilst it showed a quadratic relationship under W2, the results supported that a highly salinity-induced iWUE was dependent on water supply. In addition, although salinity had a negative correlation with fruit fresh mass (FM), dry matter content, and water content (WC) under W0 and W1, fruit titratable acidity (TA) and total soluble solids (TSS) were proportional to salinity regardless of water regimes. The results also showed that W1 increased the sensitivity of TA and TSS to salinity, but W2 resulted in weak sensitivity of FM and WC to salinity. Thus, we deduced that mildly reduced irrigation was an appropriate strategy to regulate iWUE and fruit quality under high salinity conditions. This study provides a water regime management basis for comprehensive changes in yield, WUE, and fruit quality of tomatoes grown in saline soils.

Characterization of Fruit Quality Traits and Biochemical Properties in Different Myanmar Mango Cultivars during Ripening Stages

Here, we characterized the changes in fruit quality and biochemical parameters in four Myanmar mango cultivars from ripening stage 1 to 4 at ambient temperature. Total soluble solids, total sugars, and reducing and non-reducing sugar content increased, whereas titratable acidity decreased with increasing storage time in all cultivars. ‘Sein Ta Lone’ showed the highest consumer acceptability, with maximum sensory quality scores owing to its unique characteristics. ‘Hin Thar’ and ‘Ma Chit Su’ also had better quality and sensory attributes than ‘Yin Kwae’. Sugar/acid ratios in all cultivars ranged from 23 to 50, the standard sugar/acid ratios in high-quality mango fruits. The total phenolic content (TPC) and antioxidant activity among cultivars ranged from 8.20 to 14.96 mg gallic acid equivalents and 19.52 to 26.79 mg vitamin C equivalents antioxidant capacity, respectively, per 100 g of fruit extract throughout the storage. ‘Hin Thar’ was the richest in phytochemical compounds. A significant positive correlation was found between total phenolic activity and 2,2-diphenyl-1-picryl-hydrazyl free radical scavenging activity of fruits, showing that TPC exhibited linear relationships with the antioxidant activities of each mango variety during the different stages of ripening.

The effect of concentration calcium chloride and immersion time on changes in colour and texture of fresh-cut mangosteen

Fresh-cut mangosteen is a popular product, containing functional components that are good for health. The weakness is the deterioration of colour and texture. The application of calcium chloride can delay the deterioration of fresh-cut mangosteen. This study aimed to determine the effect of the concentration of calcium chloride and immersion time on changes in the colour and texture of fresh-cut mangosteen in cold storage. Preparation of fresh-cut mangosteen was carried out by removing the skin with the application of calcium chloride solution using concentrations of 0.5, 1.0, 1.5, and 2.0% (w/v) and immersion times of 10, 20, and 30 mins. The design used was completely randomized with three replications to observe changes in browning and quality namely texture, acidity, total dissolved solids, water content, and weight loss. The treatment of calcium chloride concentration, immersion time, and their interactions affected all variables of browning and quality changes of fresh-cut mangosteen. To conclude, the best treatment that can delay browning and the quality changes of the fresh-cut mangosteen fruit is a concentration of 1% calcium chloride with an immersion time of 10 mins to 9 days at a cold storage temperature of 7±1°C.

Orchard grass safeguards sustainable development of fruit industry in China

Orchard grass has received extensive attention in recent years due to its environmental-friendly features, yet the comprehensive effects of yield, quality, environment and economics in grass-planted orchard in the long term don't still remain fully understood. The study quantified the yield, quality, environmental and economic effects of orchard grass, aiming to provide a reference for the application of grass for fruit production in China. Meta-analysis results firstly illustrate that the application of orchard grass has positive effects on fruit yield increase and quality improvement. The underlying drivers of these positive changes in fruit yield and quality are enhancements of soil properties in orchard. Secondly, long-term planting of orchard grass positively impacts soil properties linked to healthy soil and warrants fruit production. Finally, cost-benefit analysis reveal that the economic benefits of planting grass in orchards are 1.36%–2.08% higher than that of clear tillage control. Therefore, orchard grass is a sustainable safeguard to promote the development of fruit industry in the long run. Many corresponding measures should be taken to rapidly promote green manure areas and limit further increase of chemical fertilizer to maintain the sustainability of orchards.

Quality changes of acerola fruit harvested at different maturity stages and exposed to external ethylene

Although acerola (Malpighia emarginata D.C.) has been suggested to be a climacteric fruit, little is known about its sensitivity to exogenous ethylene. The objective of the study was to evaluate the quality changes of acerola fruit at two harvest maturity stages in response to external ethylene application. ‘Flor Branca’ and ‘Junko’ acerolas were harvested at the maturity stages 1 (green fruit with density>1 g cm-3) and 2(green fruit with density <1 g cm-3) and were treated with 0 or 1,000 µL L-1 of ethylene for 24 hours at 12 ºC.The fruit were stored at 12 ºC with relative humidity of 90-95% for 14 days. Both cultivars harvested at the maturity stage 2 showed skin color change from green to red during storage, which was not observed in fruit harvested at the maturity stage 1.External ethylene had no effect on 'Flor Branca' and 'Junko' acerolas respiration rate, flesh firmness, skin color, weight loss, soluble solids (SS), titratable acidity (AT), SS/AT ratio, and ascorbic acid contents. The classification of green acerolas by density was an effective approach to determine fruit harvest maturity for fresh consumption.

Melatonin stimulates postharvest ripening of apples by up-regulating gene expression of ethylene synthesis enzymes

Melatonin has been implicated in the regulation of ripening in several types of climacteric fruits, but its role in apple is poorly understood. In this study, the effectiveness of melatonin in influencing postharvest ripening in apples and interacting with ethylene biosynthesis process was investigated. Apples of four local cultivars (Golden, Reineta, Blanquina and Rabiosa) were harvest at an immature green stage and immersed for 120 min in a solution with water (control), melatonin 50 µM, ACC 1 mM, and melatonin 50 µM + ACC 1 mM. The evolution of apple quality parameters and ethylene production was assessed in all cultivars for 35 days after treatment. In addition, Golden apple was used to examine the transcript abundance of ethylene biosynthesis enzymes genes in the skin and flesh. Exogenous melatonin improved apple quality during postharvest ripening and stimulated ethylene production in all cultivars studied, advancing the timing of the climacteric burst. Melatonin-induced changes in ethylene production correlated well with changes in fruit quality and were in part similar to those induced by the ethylene precursor ACC, so ethylene appears to be a target of melatonin. In Golden apple, melatonin stimulated the expression of MdACS1 and MdACO1 genes, which showed a climacteric profile similar to that of ethylene. The transcriptional effects of exogenous melatonin on ethylene-related genes was also more notable in the skin than in the flesh, suggesting an initiating role of the former in the biosynthesis of the phytohormone. Taken together, the results point to the fact that exogenous melatonin may be useful in improving apple quality during postharvest ripening by promoting colour and flavour change and extending fruit firmness. The effect of melatonin appears to be mediated in part by up-regulation of the transcriptional activity of the MdACS1 and MdACO1 genes during the climacteric peak, thus contributing to an increase in ethylene availability.

Field inoculation responses of arbuscular mycorrhizal fungi versus endophytic fungi on sugar metabolism associated changes in fruit quality of Lane late navel orange

Arbuscular mycorrhizal (AM) fungi and plants require sugars, especially during fruit ripening stage, while the underlying mechanism influencing sugar accumulation in response to AM fungi is still unclear. The objective of this study was to analyze the field inoculation response of three AM fungi (Acaulospora scrobiculata, Diversispora spurca, and D. versiformis) and an endophytic fungus (Piriformospora indica) on internal as well as external quality parameters of Lane late navel orange (Citrus sinensis) fruits, with emphasis on monosaccharide and disaccharide components and expressions of sucrose-associated enzyme genes and sugar transporter genes. Root fungal colonization rate increased by 51.2−109.6% with the inoculation of D. spurca displaying the best response after two years of field inoculation. The fungal introduction also improved the external fruit quality attributes to some extent, especially longitudinal and transverse diameter, sarcocarp weight, and fruit coloration value in response to inoculation of A. scrobiculata and P. indica. Fruit number increased in response to inoculation with D. versiformis. Although none of the four symbiotic fungi influenced the soluble solids of fruits, however fruit titratable acids registered a significant reduction leading to an increase in soluble solids-titratable acids ratio in response to A. scroblculata and D. spurca. Inoculation with A. scrobiculata and D. spurca also reduced sucrose while increased glucose, fructose, and trehalose concentrations in fruit sarcocarps, along with up-regulated expressions of CsVINV1, CsA/NINV4, and CsSWEET in fruit sarcocarps, suggesting that AM fungi played a pivotal role in sucrose transport, synthesis, and cleavage in fruits. In contrast, inoculation with P. indica only increased the sucrose and up-regulated expressions of CsSPS1 and CsSPS4 in fruit sarcocarps, implying that Pi mainly triggered the sucrose synthesis in fruits. These symbiotic fungi increased concentration of one of the three sweeteners (arabinose, xylitol and fucose) in fruit sarcocarps, with an exception of A. scrobiculata responding to an increase in the three sweeteners. These results provided strong clues that symbiotic fungi have an important role in improving the fruit size and different sugar components in citrus fruits by regulating the expression of genes related to sucrose synthesis, cleavage, and transport, with A. scrobiculata outplaying the response of other three symbiotic fungi.

Blue mold control and volatile compounds in “Fuji” apples treated with chitosan emulsion combined with essential oils

The aim of this work was to evaluate the effects of chitosan emulsion (CE) in combination with essential oils (Eos) on blue mold control, as well as on aroma and quality attributes of “Fuji” apples after storage. Treatments evaluated were control, CE (1%) and CE (1%) combined with citronella, clove and copaiba EOs (all EOs at 500 μl L−1). Fruits treated with EC and EC + OEs showed lower total phenolic compounds in the peel in all treatments. Fruits treated with EC combined with citronella OEs (242.4 OD mg–1 of protein min–1) and clove (205.8 OD mg–1 of protein min–1) showed lower peroxidase activity in fruit peel tissue. Therefore, CE and CE combined with citronella, clove and copaiba essential oils reduced the development of blue mold in “Fuji” apples. In addition, the treatments with lower severity of blue mold showed lower peroxidase activity in the peel and pulp tissues of the fruit, indicating induction of resistance by essential oils. Novelty impact statement Chitosan biofilm reduces rot by P. expansum in “Fuji” apples, and when in addition to chitosan, essential oils of citronella, clove and copaiba are used, there was an increase in the reduction of disease severity. The aroma of the fruit did not present a characteristic aroma of the essential oils used with the method of application used. There were no changes in fruit quality attributes with the use of chitosan and chitosan biofilm with the addition of essential oils.

Influence of blanching on the bioactive compounds of Terminalia ferdinandiana Exell fruit during storage

Changes in quality of fruits and vegetables during processing and storage might impact on the nutritional and economical value of food products. The present study aimed to evaluate the influence of blanching on the content of vitamin C and ellagic acid (EA) as the main bioactive compounds present in Kakadu plum (KP) fruits (Terminalia ferdinandiana) during storage at 40oC to mimic typical temperature when wild harvested. Changes in the profile of fatty acids, malondialdehyde (MDA) production, as a biomarker for lipid peroxidation, and antioxidant properties of KP fruits were evaluated. The results revealed that vitamin C decreased between 25 and 52% over the storage period. Statistically significant differences in the concentration of vitamin C were associated with temperature (p < 0.05) and blanching (p < 0.05), whereas no significant differences in EA during storage were observed. DPPH radical scavenging capacity and total phenolic content of both blanched and control samples decreased by 80% and 35%, respectively, at the end of the storage period compared to day 0. In addition, the change in DPPH activity is significantly correlated (Pearson R2 = 0.829, p ˂ 0.01) with the breakdown of ellagitannins. Furthermore, KP fruit demonstrated excellent antioxidative properties by reducing MDA production. It was concluded that blanching causes significant vitamin C loss whereas neither blanching nor long-term storage at elevated temperature affect the EA content. The results also indicate that the antioxidant compounds present in the KP fruits provided considerable protection against fatty acid oxidation during storage.

Changes in the Primary Metabolites of 'Fengtang' Plums during Storage Detected by Widely Targeted Metabolomics.

Plums are one of the most popular stone fruits worldwide owing to their high nutritional value. After harvest, plum fruit quality and flavor change during storage; however, little is known about the changes in metabolites during this period. A comprehensive comparison of primary metabolites in ‘Fengtang’ plum fruits during storage is performed using widely targeted primary metabolomics. A total of 272 primary metabolites were identified by means of ultra-performance liquid chromatography and tandem mass spectrometry (UPLC-MS/MS) in the plums at different storage periods. There was a significant increase in the relative amounts of twenty-eight lipids, twenty amino acids and their derivatives, thirteen organic acids, ten saccharides and alcohols, six nucleotides and their derivatives, and two vitamins. A Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of differential metabolites revealed that glucosinolate biosynthesis, starch and sucrose metabolism, ascorbate and aldarate metabolism, lysine degradation, and other metabolic pathways were significantly enriched; therefore, changes in these metabolic pathways may be key to the quality and flavor change in ‘Fengtang’ plum fruits during storage. Our results provide a theoretical foundation and technical support to evaluate ‘Fengtang’ plum fruit quality.