Stages Of Fruit Ripening Research Articles

Characterization of the Passion Fruit (Passiflora edulis Sim) bHLH Family in Fruit Development and Abiotic Stress and Functional Analysis of PebHLH56 in Cold Stress

Abiotic stress is the focus of research on passion fruit characters because of its damage to the industry. Basic helix-loop-helix (bHLH) is one of the Transcription factors (TFs) which can act in an anti-abiotic stress role through diverse biological processes. However, no systemic analysis of the passion fruit bHLH (PebHLH) family was reported. In this study, 117 PebHLH members were identified from the genome of passion fruit, related to plant stress resistance and development by prediction of protein interaction. Furthermore, the transcriptome sequencing results showed that the PebHLHs responded to different abiotic stresses. At different ripening stages of passion fruit, the expression level of most PebHLHs in the immature stage (T1) was higher than that in the mature stage (T2 and T3). Eight PebHLHs with differentially expressed under different stress treatments and different ripening stages were selected and verified by qRT-PCR. In this research, the expression of one member, PebHLH56, was induced under cold stress. Further, the promoter of PebHLH56 was fused to β-Galactosidase (GUS) to generate the expression vector that was transformed into Arabidopsis. It showed that PebHLH56 could significantly respond to cold stress. This study provided new insights into the regulatory functions of PebHLH genes during fruit maturity stages and abiotic stress, thereby improving the understanding of the characteristics and evolution of the PebHLH gene family.

Estimating the Effects of Deficit Irrigation on Water Absorption and Utilization of Tomatoes Grown in Greenhouse with Hydrus-1D Model

Quantitative analysis of tomato root water uptake and soil water utilization in the root zone under deficit irrigation is an important tool to improve agricultural water utilization efficiency. In this study, three different deficit irrigation levels were set at the flowering and fruit development stage (Stage I) and the fruit-ripening stage (Stage II), respectively. The Hydrus-1D model and field data were used to analyze the effects of deficit irrigation on tomato root growth, soil water uptake and utilization in the root zone. The results showed that deficit irrigation could reduce the total root length density of water-absorbed roots but increase the water-absorbed root length density of the underlying soil (30–60 cm). Moderate and severe water deficits at Stage II increased the water-absorbed root length density of the underlying soil by 0.10–6.26% and 2.12–11.71% compared with a mild water deficit. Considering tomato root growth, the Hydrus-1D model can improve the accuracy of soil moisture simulation. The main water absorption zone of tomato roots was 0–30 cm. Compared with full irrigation, the ratio of water absorption by the underlying root system (30–60 cm) to the total water absorption of the profile (0–60 cm) increased by 2.16–2.82% and 5.34–6.34% due to mild and moderate water deficits at Stage I. At Stage I and Stage II, a water deficit could reduce soil evaporation. T3 had the highest water use efficiency in two years, which was 24.07% (T9) and 20.47% (T8) higher than the lowest value, respectively. The optimal deficit irrigation scheme under this experiment condition is as follows: the soil water content was 70–90% θf (field capacity) at Stage I and was 40–60% θf at Stage II (T3).

Dynamics of Nutrients in Jujube (Ziziphus jujuba Mill.) at Different Maturity Stages, Cultivars, and Locations in the Southwest United States

Jujube (Ziziphus jujuba Mill.) is also called Chinese date. There are ∼100 jujube cultivars with limited commercial availability, and the majority of them have scant details in the United States. In this study, nutrient dynamics during fruit maturation of different jujube cultivars grown at Las Cruces, Los Lunas, and Alcalde, NM, were examined in 2018 and 2019. Cultivars varied by location and year, and included ‘Li’, ‘Lang’, ‘Sugarcane’, ‘September Late’, and ‘Sherwood’. Parameters tested were total phenolic content (TPC), proanthocyanidins (PAs), vitamin C, cyclic adenosine monophosphate (cAMP), and antioxidant capacity: 2,2-diphenylpicrylhydrazyl radical scavenging capacity and ferric reducing antioxidant potential (FRAP). Moisture, TPC, PAs, FRAP, and vitamin C content decreased with fruit maturity; however, the latter stage of fruit maturity showed an increase in cAMP. Compared with fruit at full-red maturity, creamy fruit had TPC, PA, FRAP, and vitamin C concentrations that were 1.0 to 1.8, 4.4 to 12.4, 1.9 to 2.6, and 0.1 to 1.3 times higher, respectively, depending on location (P < 0.05). From creamy to full-red maturity, cAMP increased by 0.9 to 4.5 times. At full-red maturity, estimated TPC in jujube fruit ranged from 10.6 to 16.8 mg gallic acid equivalent per gram dry weight (DW), whereas estimated PAs ranged from 1.8 to 5.3 mg PA B2/g DW. Jujube fruit at full-red maturity had a vitamin C content that ranged from 649.0 to 1153.3 mg/100 g DW. At full-red maturity, the concentration of cAMP ranged from 148.1 to 277.6 μg/g DW in Las Cruces samples.

Comparison of Nutrient Composition and Antioxidant Activity of Hydroponically Grown Commercial and Traditional Greek Tomato Cultivars

The consumer demand for an enhanced diet intake of antioxidants and bioactive compounds is continuously rising. This work aims to evaluate the fruit nutritional composition and antioxidant activity of five tomato germplasm varieties, alongside a commercial F1 hybrid. Three varieties bear small-fruit (14–40 g), while two varieties and the commercial cultivar yield large fruit (150–300 g). Genotypes under study were cultivated in a greenhouse under the same environmental conditions. Fat, protein, carbohydrate, total phenol, total flavonoid, lycopene, and ascorbic acid contents were assessed at two fruit maturity stages (breaker, red ripe). For both hydrophilic and lipophilic fractions, antioxidant behavior was also evaluated by employing DPPH and FRAP assays. Small-fruit varieties generally possess higher fat and ascorbic acid content, as well as hydrophilic FRAP values as compared to large-fruit ones. In all varieties, lycopene content and lipophilic fraction radical scavenging capacity was considerably higher at red ripe stage. At red ripe stage, all germplasm varieties were clearly and consistently superior in terms of antioxidant activity at the lipophilic fraction owing to enhanced lycopene content. The results emphasize the value of reintroducing germplasm varieties in breeding programs and suggest that local varieties generally encompass high quality features.

High-Relative-Humidity Storage Reduces the Chilling Injury Symptoms of Red Sweet Peppers in the Breaker Stage

Water loss, interwoven with other factors, is identified as the cause of chilling injury to sweet peppers. The breaker stage is the most vulnerable of all maturity stages. The purpose of this study was to see if increasing the storage relative humidity (RH) reduces the chilling injury and to compare its effect on breaker-stage fruits to red-ripe fruits at a normal RH. The fruits were stored at 5 °C with a RH of 98 ± 2% and 70 ± 6% for high and low RH, respectively. After 15 days of cold storage, the fruits were moved to ambient conditions for 3–5 days for chilling injury symptoms to appear. The results showed that high RH storage reduced fruit water loss by 4–4.5% compared to low RH storage, resulting in fewer chilling injury symptoms regardless of fruit maturity stage. Due to the increased RH, cell membrane damage indicators such as electrolyte leakage, MDA, respiration, and ethylene production rates were shown to be reduced, while brix and color were well maintained, indicating reduced or stopped senescence. Furthermore, DPPH antioxidant activity and vitamin C were retained and optimized. The microbiological analyses also showed that a high RH may not promote the growth of microorganisms as quickly as may have been thought. Finally, the findings of this study indicate that breaker-stage peppers stored at a high RH may be less susceptible to chilling injury than red-ripe peppers stored at a low RH.

Comprehensive fruit quality assessment and identification of aroma-active compounds in green pepper (Capsicum annuum L.).

The wrinkled pepper (Capsicum annuum L.) is a type of chili pepper domesticated in northwestern China, with a characteristic flavor. Fifteen wrinkled and four smooth-skinned pepper varieties were evaluated for morphology, texture, color, nutrients, capsaicinoids, and volatile compounds at the mature fruit stage. The sensory evaluation showed wrinkled pepper was superior to smooth pepper in texture, and it has a highly significant correlation (p < 0.01) with cuticle thickness, maximum penetrating force, lignin content, and moisture content. Citric acid was the major organic acid in peppers, accounting for 39.10-63.55% of the total organic acids, followed by quininic acid. The average oxalic acid content of smooth peppers was 26.19% higher than that of wrinkled peppers. The pungency of wrinkled pepper fruits ranged from 1748.9 to 25529.4 SHU, which can be considered slightly to very spicy, while the four smooth varieties ranged between 866.63 and 8533.70 SHU, at slightly to moderately spicy. A total of 199 volatile compounds were detected in the 19 pepper varieties. The average volatile content of wrinkled pepper was 39.79% higher than that of smooth pepper. Twenty-nine volatile compounds, including 14 aldehydes, four alcohols, three esters, three ketones, two furans, one pyrazine, one acid, and one phenol, contributed to the fragrance of peppers and could be regarded as aroma-active compounds, with 2-isobutyl-3-methoxypyrazine being the major contributor among the 19 pepper varieties. Wrinkled pepper can be confidently distinguished from smooth pepper and is of superior quality. The current findings outlined the major texture-related characteristics of pepper as well as the main aroma-active compounds, providing valuable information for pepper quality breeding and consumer guidelines.

Application of meta-heuristic feature selection method in low-cost portable device for watermelon classification using signal processing techniques

Recognizing the stage of fruit maturity and thus determining the optimum harvesting time is critical since the competitive market requires high-quality products at a competitive price. Furthermore, in the context of the global water crisis, harvesting watermelons at an inappropriate ripening stage causes water wasting of around 280 kg per kilo of watermelon as a virtual water footprint, highlighting the necessity of criteria for harvesting ripe watermelons by the farmers. This research thus aims to classify the Charleston Gray watermelon type into three categories i.e. unripe, ripe, and overripe using portable acoustic signal processing, data mining methods, and artificial intelligence approaches.Signal processing in the time and frequency domains and Wavelet Transformation were used to extract essential features from acoustic signals of the watermelons. This was followed by the selection of the significant features in classification using a t-test mean comparison. Sample categorization was accomplished using Support Vector Machines and the K-Nearest Neighbor techniques. Considering the effect of three impact positions (the stem side, middle, and flower side), two materials (Polyoxymethylene and metal balls) and two diameters (15 and 10 mm diameter) for striker ball, using Principal Component Analysis and application of various signal processing methods, 3360 models were established. Based on the frequency equipped with the Mahalanobis distance, the K-Nearest Neighbor classifier presented the best result with a 77.3 % classification rate for signals obtained from the small metal ball and stem side impact position. In comparison, experts accurately had categorized 52 % of the samples in total.

Variation in physicochemical properties and bioactivities of Morinda citrifolia L. (Noni) polysaccharides at different stages of maturity.

Morinda citrifolia L. (Noni) as an evergreen plant is a rich source of natural polysaccharides. The present work aims to investigate the maturation-related changes in polysaccharides of Morinda citrifolia L. (Noni) at five stages of maturity (stages from the lowest to highest degree - 1, 2, 3, 4, and 5). The chemical composition (carbohydrate, protein, uronic acid, and sulfate radical) of Noni polysaccharides was determined by different chemical assays. Ion chromatography system was used to analyze the monosaccharide composition, and the molecular weight was measured by HPGPC. The polysaccharides were also analyzed by FT-IR and their radical scavenging effect against DPPH, hydroxyl radicals and ABTS was evaluated. The UV-vis assay and gel electrophoresis assay were performed to investigate the DNA damage protective effect. Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides. However, no fruit maturity stage had prominent impact on the sulfuric radical contents and preliminary structure characteristics. Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals. The stronger DPPH and ABTS radical scavenging activities of N5 might be contributed by its higher content of fucose and rhamnose and smaller molecular weight. Noni polysaccharides extracted at stage 4 (N4) showed the highest uronic acid content (4.10 ± 0.12%), and the superior performance in scavenging hydroxyl radicals and protecting DNA. The greater hydroxyl radical scavenging effect of N4 might be attributed to its higher percentage of the low molecular weight counterpart. Moreover, the DNA protective effects of N4 displayed a positive correlation with its hydroxyl radical scavenging ability. Overall, stage 4 and stage 5 could be ideal stages of fruit maturity aiming at high-quality Noni polysaccharides extraction. This study provided valuable information for the selection of suitable Noni polysaccharides to cater for various industrial applications.

Variations in mango fruit quality in response to management factors on a pre- and post-harvest continuum

Summary Fruit quality is a key factor – beginning with the producer, continuing through the supply chain, and ending with the consumer. It is described by multiple indicators and varies during the growth and ripening of the fruit. This study focused on two main aspects of Cogshall mango (Mangifera indica L.) quality: (i) the physical properties of the fruit with fresh mass (FM), pulp dry matter content (DMC), and pulp coloration; and (ii) the chemical properties with pulp sugar content and pulp acidity. These indicators were monitored on on-tree fruit, from about 60 days after bloom until full maturity. The same indicators were also monitored on fruit stored in cold storage rooms during ripening. The effects of leaf-to-fruit ratio (manageable by pruning or fruit thinning), maturity stage of fruit at harvest (manageable by harvest date), and storage temperature on the kinetics of quality traits of on-tree and stored fruit were assessed. In addition, a change-point analysis was applied to the sweetness index kinetics (used as a proxy of fruit ripening) to study fruit ripening induction. The leaf-to-fruit ratio mainly influenced fruit growth in terms of FM and pulp DMC, whereas it had less impact on the evolution of fruit chemical properties. The maturity stage of the fruit at harvest was a key factor in determining the potential quality at the ripe stage. Ripening occurs naturally at the mature green stage for on-tree fruit, but ripening at an earlier stage can be induced by harvesting the fruit. During the ripening phase, a low leaf-to-fruit ratio and a cold storage temperature tended to slow down the daily rate of sweetness increase. The use of cold temperatures during storage slowed down starch degradation and sucrose accumulation, while almost stopping the variation in fruit coloration and acidity.

Effect of soil water content threshold on kiwifruit quality at different growth stages with drip irrigation in the humid area of Southern China

• Effects of soil water content threshold (SWCT) on kiwifruit quality depended on SWCT timing and degree. • SWCT of 55% field capacity (FC) increased fruit volume and fruit weight at bud burst to leafing stage. • SWCT of 65% FC elevated total soluble solids, soluble sugar and solid-acid ratio at fruit growth stage. • SWCT of 60% FC improved firmness, total soluble solids, soluble sugar, vitamin C and solid-acid ratio at fruit maturation stage. Fruit quality as affected by soil water content threshold levels (SWCT) at different growth stages remains largely elusive. Therefore, a drip irrigation experiment was conducted to investigate the effect of SWCT on kiwifruit quality at bud burst to leafing stage (stage I), flowering to fruit set stage (stage II), fruit growth stage (stage III) and fruit maturation stage (stage IV) during 2018 and 2019 in the humid area of southern China, respectively. The results showed that SWCT at stage I mainly affected fruit weight (Fw). I-55% treatment significantly elevated Fw by 11.6%-8.0% ( P < 0.05). SWCT at stage II had little effect on fruit quality. SWCT at stage III influenced fruit volume (Fv) and chemical quality. Specifically, Fv was increased by 8.0%-2.2% under III-75% treatment, total soluble solids (TSS), soluble sugar (SS) and solids-acid ratio (SOAR) were increased by 9.4%-10.0% ( P < 0.05), 2.4%-5.8% and 7.9%-10.6% ( P < 0.05) under III-65% treatment, respectively. SWCT at stage IV impacted fruit firmness (Fn) and chemical quality. IV-60% treatment increased Fn, TSS, SS, VC and SOAR by 5.6%-10.5%, 12.0%-12.6% ( P < 0.05), 6.6%-9.1% ( P < 0.05), 2.1%-14.0% ( P < 0.05), and 8.1%-8.3% ( P < 0.05), respectively, which was beneficial to the improvement of nutritional value and taste, and also the storage and transportation of kiwifruit. Collectively, SWCT of 55% FC (field capacity) at stage I or 65% FC at stage III or 60% FC at stage IV was an effective measure to improve the kiwifruit quality.

Comparison of bioactive compounds of continental and Mediterranean pomegranate fruits and its application in urban gardening

With the significant increase in population in cities, the necessity of growing safe and functional food is of great importance. Pomegranate fruit has many valuable antioxidant properties. The subject of study was the pomegranate from the continental region (Sremski Karlovci, Serbia) and the Mediterranean region (Bar, Montenegro). We compared biochemically active compounds in genotypes from both locations. The analysis performed included total phenolics, tannins, flavonoid contents, proanthocyanidins, pigments chlorophyll a and chlorophyll b, as well as carotenoids. Analysis of vitamin C, titratable acidity, pH value, and total sugars were also performed. Two antioxidant tests were also conducted, including DPPH and ABTS tests. Based on the results obtained, we concluded that pomegranate grown in nonprimary conditions, such as in the continental area in Serbia, had a significantly higher amount of biochemically active compounds than the pomegranate grown in the Mediterranean urban region. It was found that the content of total phenols is significantly higher in all continental pomegranate genotypes, through all three phases, implying the adaptation of this species to continental conditions. The value of these active compounds through the second and third phases of fruit ripening is especially emphasized (ME047-1.18 mg gae/g fw; CO170-8.46 mg gae/g fw). Antioxidant tests also indicate the great potential of examined fruits, especially through the first and third stages of fruit ripening, where continental samples reached a value of 99.36 mg teac/g fw, while Mediterranean ones reached 51.84 mg teac/g fw for the ABTS assay. The proanthocyanidins content was also emphasized in continental varieties where it reached a value of CO170 (12.13 mg ce/g fw) in the third stage of ripening, while in the Mediterranean varieties the maximum was reached in the ME029 genotype (4.36 mg ce/g fw). Such results indicate the importance of growing fruit species that are adapted to urban conditions and that have been proven useful when it comes to the impact on human health in urban areas.

Qualidade fisiológica de sementes de Physalis ixocarpa Brot. ex Hornem em relação ao estágio de maturação e época de cultivo

ABSTRACT Physalis ixocarpa is an edible fruit with great economic importance in many countries. The use of high-quality seeds is essential for the success of the crop, so their harvest should be carried out at their maximum point of physiological maturity. This study aimed to verify the influence of the growing season and fruit maturation stage on the seed physiological maturity of two P. ixocarpa varieties (‘green’ and ‘purple’) grown in the Brazilian semi-arid region. The water content, dry weight, germination percentage and seedling emergence were evaluated. The growing season influenced the seed maturation process of the two evaluated varieties, with a more pronounced effect for the ‘purple’ one. To obtain higher-quality seeds, the cultivation in the Brazilian semi-arid region can be carried out from May to August and the collection of fruits to obtain seeds from the stage 4 of maturation.

Efeito do estádio de maturação de frutos e reguladores vegetais na qualidade fisiológica de sementes de Maracujá Doce

Abstract This study aimed to evaluate the effect of fruit maturation stages and pre-germination treatments with plant regulators on the physiological quality of seeds of cv. BRS Mel do Cerrado. Two experiments were carried out, germination and emergence, installed in a completely randomized design in a 3 x 2 factorial arrangement, with three stages maturation (1-partially ripe fruits; 2-fully ripe fruits and 3-senescent fruits) and two pre-germination treatments (1. [GA4+7 +N-(phenylmethyl)-aminopurine (300 ppm)] and 2. distilled water), forming six treatments with four replications of 50 seeds. The evaluated characteristics were germination percentage at 14 and 35 days, germination speed index (GSI), emergence percentage and emergence speed index (ESI). Germination at 14 days, GSI and seedling emergence were not affected by the maturation stage, however germination and GSI were higher when the plant regulator was used. The final percentage of germination was higher in seeds taken from partially ripe fruits and treated with regulators and a higher ESI was observed for seeds from senescent fruits and treated with regulator. Seeds treated with plant regulators are more vigorous and have a higher rate of seedling emergence.

Characterization and classification of lulo (Solanum quitoense Lam.) fruits by ripening stage using partial least squares discriminant analysis (PLS-DA)

Lulo or naranjilla (Solanum quitoense Lam.) is a tropical fruit with great potential for its contents of antioxidant and biofunctional compounds and sensory characteristics. Nowadays, the different methodologies to classify the ripening stage of lulo fruits are prone to bias and can hinder adequate characterization of the fruit maturity stage as they do not use measurements. The aim of this research was to define an accurate method for classifying lulo fruits by ripening stage based on non-destructive parameters and to determine their main characteristics according to the ripening stage. Hierarchical cluster analysis was carried out to classify fruits according to their maturity index (MI) into two (MI2) and three (MI3) homogeneous groups of individuals. Using partial least squares discriminant analysis (PLS-DA), with the non-destructive parameters showing significant differences between groups, classification functions by ripening stage were established. The PLS-DA correctly classified 89.47% of the fruits in the MI2 classification and 78.95% in the MI3 classification. The predictive power of the models was tested with fruits other than those used to establish the prediction equations, obtaining a correct classification in 75% of the cases. It is possible to classify lulo fruits objectively with a limited number of non-destructive parameters that constitutes a useful tool from harvesting to consumption.

Genes related to cell wall metabolisms are targeted by miRNAs in immature tomato fruits under drought stress.

The metabolism of tomato fruits changes when plants experience drought stress. In this study, we investigated changes in microRNA (miRNA) abundance and detected 32 miRNAs whose expression changes in fruit. The candidate target genes for each miRNA were predicted from the differentially expressed genes identified by transcriptome analysis at the same fruit maturation stage. The predicted targeted genes were related to cell wall metabolisms, response to pathogens, and plant hormones. Among these, we focused on cell wall metabolism-related genes and performed a dual luciferase assay to assess the targeting of their mRNAs by their predicted miRNA. As a result, sly-miR10532 and sly-miR7981e suppress the expression of mRNAs of galacturonosyltransferase-10 like encoding the main enzyme of pectin biosynthesis, while sly-miR171b-5p targets β-1,3-glucosidase mRNAs involved in glucan degradation. These results will allow the systematic characterization of miRNA and their target genes in the tomato fruit under drought stress conditions.

Transcriptome and metabolome analyses reveal anthocyanins pathways associated with fruit color changes in plum (Prunus salicina Lindl.).

Plum (Prunus salicina Lindl.) is one of the most widely cultivated and important fruit trees in temperate and cold regions. Fruit color is a significant trait relating to fruit quality in plum. However, its development mechanism has not been studied from the aspects of transcriptional regulation and metabolomic progress. To reveal the mechanism of fruit color developments in plums, we selected the fruits of two plum cultivars, 'Changli84' (Ch84, red fruit) and 'Dahuangganhe' (D, yellow fruit) as plant materials for transcriptome sequencing and metabolomic analysis were performed. Based on the data of transcriptome and metabolome at three fruit developmental stages, young fruit stage, color-change stage, and maturation stage, we identified 2,492 differentially expressed genes (DEGs) and 54 differential metabolites (DMs). The KEGG analysis indicated that "Flavonoid biosynthesis" was significantly enriched during three fruit development stages. Some DEGs in the "Flavonoid biosynthesis" pathway, had opposite trends between Ch84 and D, including chalcone synthase (CHS), dihydroflavonol 4-reductase (DFR) and flavonol synthase (FLS). Also, the genes encoding MYB-bHLH-WD (MBW) protein complexes, especially MYBs and bHLHs, showed a close relationship with plum fruit color. In the current study, DMs like procyanidin B1, cyanidin 3-glucoside, and cyanidin-3-O-alpha-arabinopyranoside were key pigments (or precursors), while the carotene and carotenoids did not show key relationships with fruit color. In conclusion, the anthocyanins dominate the color change of plum fruit. Carotenes and carotenoids might be related to the color of plum fruit, but do not play a dominate role.

Comparative analysis of anthocyanin content in various tomato samples in connection with breeding

Relevance. Anthocyanin pigments can be synthesised in the stem, leaves, calyx, peel and pulp of tomato fruit. These compounds protect the photosynthetic apparatus of the plant, neutralise free radicals, increase the efficiency of phosphorus and nitrogen assimilation, have an osmoregulatory function, antimicrobial activity and increase the level of adaptation to unfavourable environmental conditions. In addition, anthocyanins from plant foods play an important role in the prevention of type II diabetes, neurodegenerative processes, cardiovascular and infectious diseases. Therefore, the cultivation of tomatoes with anthocyanin-coloured fruits is a perspective area for the vegetable industry in Russia. Vegetable products containing antioxidant substances belong to the functional products and conform to the concept of a healthy diet. As one of the most popular and frequently consumed vegetable crops, the anthocyanin content of tomato is advisable to increase in the fruit (peel and pulp).Material and methods. In this work we studied breeding samples of FSBSI FSVC: L-Ch-365, L-IR-2080, L-Zemba, hybrid combination F1 Zemba x L-Ch-365, variety of Siberian Botanical Garden Bosare blue. The aim of our research was to determine the quantitative composition of phenolic compounds - anthocyanins in different tomato samples, and on this basis to determine the objectives of breeding to improve the content of phenolic compounds.Results. As a result of the research, the quantitative content of anthocyanins in fruits of dif- ferent tomato specimens has been studied, on which the dietary and medicinal quality of the fruits depends. The sources of high content of anthocyanins (L-Zemba, L-IR-2080) have been obtained, which will make possible to conduct directed breeding work for high content of phenolic compounds. It has been shown that anthocyanin-rich tomato samples can be rich in carotenoids, which gives an opportunity to create new varieties and hybrids with high content of water- and fat-soluble antioxidants. Potential samples for breeding according to the complex of biochemical indicators have been identified: L-Zemba, L-IR-2080, Bosare blue. It has been shown that selection for high content of anthocyanins can be performed at various stages of fruit ripening (large ripe fruit, blangetongue, biological ripeness), selecting the most intense colouring. The Zemba tomato variety developed at FSBSI FSVC has a sufficiently high anthocyanin content and is recommended for cultivation in production.

Diversity Analysis of Leaf Nutrient Endophytes and Metabolites in Dioecious Idesia polycarpa Maxim Leaves during Reproductive Stages.

Leaves are essential vegetative organs of plants. Studying the variations in leaf nutrient content and microbial communities of male and female plants at reproductive stages helps us understand allocation and adaptation strategies. This study aimed to determine the nutrient characteristics and microbial differences in the leaves of male and female Idesia polycarpa at reproductive stages. Seven-year-old female and male plants were used as test materials in this experiment. The samples were collected at three stages: flowering (May), fruit matter accumulation (July), and fruit ripening (October). The nitrogen (TN), phosphorus (TP), potassium (TK), carbon (TC), and the pH of the female and male leaves were analyzed. In addition, the leaf microbial diversity and differential metabolites were determined using the Illumina high-throughput sequencing method and the ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method at the reproductive developmental stages. This study found that male and female plant leaves had different TN and TK contents over time but no difference in TC and TP content. The significant differences in bacterial diversity between male and female plants and the richness of the fungi of male plants at the flowering and fruit maturity stages were observed. Proteobacteria, Pseudomonadaceae, Ascomycota, and Aspergillus were the dominant bacteria and fungi in the Idesia polycarpa leaves. The presence of microorganisms differed in the two sexes in different periods. Alphaproteobacteria and Sordariomycetes were the indicator groups for male leaves, and Pseudomonas and Sordariomycetes were the indicator groups for female leaves. Significant differences in phenolic acid were found between male and female leaves. A KEGG enrichment analysis revealed that differential metabolites were enriched in metabolic pathways, amino acid biosynthesis, and the nucleotide metabolism. According to a correlation analysis, leaf TK and TP were strongly correlated with endophytic bacteria abundance and differential metabolite composition. This study revealed the changes in substances and microorganisms in the leaves of male and female plants in their reproductive stages. It provides a theoretical basis for developing and utilizing the leaves of Idesia polycarpa and for field management.

Identification of Phenological Growth Stages of Four Morus Species Based on the Extended BBCH-Scale and Its Application in Fruit Development with Morphological Profiles and Color Characteristics

Mulberry (Morus L.; Moraceae; Rosales) is an economically important tree with a long history in China and valued for its rich nutrient, flavor content, medicinal value and useful ecological functions. Morus species are widely distributed in Asia, Europe, North and South America and Africa and exhibit obvious differences in morphological characteristics and phenological stages due to being distributed in diverse climate zones. The morphological description of the phenological stages of black mulberry (Morus nigra L.) has been established in Mediterranean climates and this study extended the BBCH scale for application in different Morus species, especially for mulberry trees grown in subtropical monsoon climates. In this study, we used the BBCH (Biologische Bundesanstalt, Bundessortenamt and CHemische Industrie) scale to describe in detail the phenological growth stages for Morus species in Wuhan, China (a subtropical monsoon climate). Based on this general scale, eight principal stages, i.e., bud, leaf and shoot development stage, inflorescence emergence stage, flowering stage, fruit development stage, fruit maturation stage, senescence and beginning of dormancy stage, were described. We provide photographic images of some primary and secondary developmental stages to better define and standardize morphological characteristics and phenological descriptions of these mulberries (Morus spp.). In addition, because the color of mulberry fruit varies from white, purple, black, or pink to red at maturity, with mostly monosexual flowers, we have also presented detailed descriptions of morphological traits of flower sexuality and fruit color among these four species. In addition, two late phenological germplasms (late bud sprouting associated with late blooming and late fruit ripening) were screened out according to the BBCH scale. Overall, this study will contribute to advance the field of mulberry breeding and implementation of agronomic practices and facilitate convenient communication between mulberry cultivators and researchers in different areas.

Profiling Primary Metabolites of Governor’s Plum Flacourtia indica (Burm.f.) Merr. at Two Different Ripe Stages

To date, no study has investigated the variation of the primary metabolite profile of the fruit of Flacourtia indica (Burm.f.) Merr. (commonly known as governor’s plum), an underutilised fruit in Jamaica. To fill this gap, the current study aimed to bring novel data on this fruit at two different ripe stages (light = deep wine-red colour and dark = fully darkened brown colour) and explore the variation of their metabolome profiles. The gas chromatography-mass spectrometry (GC-MS) profiling identified 10 saccharides, 4 sugar alcohols, 11 organic acids, 24 fatty acids, and 8 amino acids in the light and dark colour fruits. However, some metabolites were not shared by both fruit ripening stages. The principal component analysis (PCA) of the different classes of the primary metabolites showed that the significant difference between the light and dark colour governor’s plum fruit is mainly determined by the content of sugars and organic acids, with the fully ripe (dark) stage expressing significant high levels of both. The hierarchical cluster analysis (HCA) showed that the profiled sugars, sugar alcohols, and fatty acids were grouped into two main clusters. In contrast, organic acids and amino acids were grouped into one cluster. However, some metabolites were related to the clusters observed. With these profiles, it was concluded that the dark colour governor’s plum is in the true ripe stage, although the light colour fruit is commercially considered ripe.