Fruit Growth Research Articles

Influence of naphthalene acetic acid, gibberellic acid and triacontanol on fruit retention, yield and quality of mango (Mangifera indica L.) cv. Banganpalli

A field investigation was carried out on fifteen-year-old mango cv. Banganpalli during 2021-2023 in the coastal region of India at the research farm of ICAR-IIHR-Central Horticultural Experiment Station, Bhubaneswar, Odisha to evaluate the efficacy of some plant growth regulators, namely naphthalene acetic acid (NAA), gibberellic acid (GA3) and triacontanol (TRIA) for improving fruit retention, yield and quality of harvest. The experiment was laid out in randomized block design with 10 treatments consisting of NAA (10, 20 and 30 ppm), GA3 (25, 50 and 75 ppm), TRIA (1, 3 and 5 ppm) and water spray as control. Each treatment was replicated thrice with four plants per replication. Treatments were applied on the plant canopy thrice at panicle initiation, pea and marble stages of fruit growth. Observations were recorded on flowering, fruiting, yield and fruit quality indices. Application of triacontanol at 3-5 ppm brought out a significant improvement over control in terms of panicle size, fruit retention and yield. Plants sprayed with 5 ppm triacontanol produced the largest panicle (length: 29.17 cm, width: 18.07 cm) and recorded the maximum value for fruit retention (68.93, 53.38, 39.41, 32.88, 26.54, 20.46 and 16.58% at 15, 30, 45, 60, 75, 90 and 105 days after pea stage, respectively), number of fruits/tree (104.72) and yield (38.95 kg/tree). With respect to fruit quality, GA3 and TRIA exhibited significant influence on fractions of fruit, dry matter and on most of the chemical attributes (TSS, TSS/acid ratio, total sugar, reducing sugar, non-reducing sugar and vitamin C) when applied at the concentration of 50-75 and 3-5 ppm, respectively.

Roles and Regulations of Acid Invertases in Plants: Current Knowledge and Future Perspectives

Acid invertases (Ac-Invs) are crucial enzymes in plant physiology, regulating sucrose metabolism and hydrolyzing sucrose into glucose and fructose. These sugars serve not only as energy sources and structural components but also as signaling molecules, influencing diverse developmental processes, including seed and fruit growth, flowering, and stress responses. Ac-Invs are classified into cell wall invertases (CWINs) and vacuolar invertases (VINs) based on their subcellular localization, with both playing distinct roles in sucrose unloading, osmotic regulation, and sugar accumulation. Recent studies have also highlighted their involvement in abiotic stress adaptation and hormonal regulation, emphasizing their central role in plant resilience and productivity. However, gaps remain in understanding their regulatory mechanisms, particularly their interactions with plant hormones, defective invertases, and responses to environmental stresses. This review summarizes the biochemical characteristics, functions, and regulatory mechanisms of Ac-Invs, providing insights into their evolutionary significance and potential applications in crop improvement. Future research directions are proposed to elucidate unresolved questions and leverage Ac-Invs for enhancing agricultural sustainability.

A Study of the Different Strains of the Genus Azospirillum spp. on Increasing Productivity and Stress Resilience in Plants.

One of the most important and essential components of sustainable agricultural production is biostimulants, which are emerging as a notable alternative of chemical-based products to mitigate soil contamination and environmental hazards. The most important modes of action of bacterial plant biostimulants on different plants are increasing disease resistance; activation of genes; production of chelating agents and organic acids; boosting quality through metabolome modulation; affecting the biosynthesis of phytochemicals; coordinating the activity of antioxidants and antioxidant enzymes; synthesis and accumulation of anthocyanins, vitamin C, and polyphenols; enhancing abiotic stress through cytokinin and abscisic acid (ABA) production; upregulation of stress-related genes; and the production of exopolysaccharides, secondary metabolites, and ACC deaminase. Azospirillum is a free-living bacterial genus which can promote the yield and growth of many species, with multiple modes of action which can vary on the basis of different climate and soil conditions. Different species of Bacillus spp. can increase the growth, yield, and biomass of plants by increasing the availability of nutrients; enhancing the solubilization and subsequent uptake of nutrients; synthesizing indole-3-acetic acid; fixing nitrogen; solubilizing phosphorus; promoting the production of phytohormones; enhancing the growth, production, and quality of fruits and crops via enhancing the production of carotenoids, flavonoids, phenols, and antioxidants; and increasing the synthesis of indoleacetic acid (IAA), gibberellins, siderophores, carotenoids, nitric oxide, and different cell surface components. The aim of this manuscript is to survey the effects of Azospirillum spp. and Bacillus spp. by presenting case studies and successful paradigms in several horticultural and agricultural plants.

Fade into you: genetic control of pigmentation patterns in red-flesh apple (Malus domestica).

The genetic basis of type 1 red-flesh color development in apple (Malus domestica) depends upon a particular allele of the MdMYB10 gene. Interestingly, type 1 red-flesh apples are fully red after fruit set, but anthocyanin pigmentation in apple fruit cortex may decrease during fruit growth and maturation, leading to variable red patterning and intensities in the mature cortical flesh. We developed a histogram-based color analysis method to quantitatively estimate pigmentation patterns. This methodology was applied to investigate the phenotypic diversity in four hybrid F1 families segregating for red-flesh color. Pigmentation patterns were found to be heritable allowing the identification of a new locus by QTL analysis. To further investigate the mechanisms involved in the spatial deposition of anthocyanin, metabolome, transcriptome and methylome comparisons between white and red flesh areas within the red-flesh genotype cv. 'R201' exhibiting flesh pigmentation patterns, was performed. Wide-targeted analysis showed that white-flesh areas accumulate more dihydrochalcones and hydroxycinnamic acids than red-flesh areas while red-flesh areas accumulate more flavonoids. Anthocyanin biosynthesis genes and anthocyanin positive regulators (MBW complex) were up-regulated in red-flesh areas, while a reduction in anthocyanin storage, transport and stability (increase of pH, down-regulation of MdGSTU22) and an increase in phenolic catabolism were concomitant with color fading process in white-flesh areas. Expression of MdGSTU22 was linked to a differentially methylated region (DMR) suggesting a potential environmental effect on the epigenetic control of gene expression involved in color fading. Altogether, these results provide the first characterization and functional identification of color fading in apple fruit flesh.

Yellow Pitahaya (Selenicereus megalanthus Haw.): The Less Known of the Pitahayas.

Pitahaya or dragon fruit is an exotic fruit native to Mesoamerica and is cultivated in several regions of the world. In recent years, pitahaya has become increasingly in demand, firstly, for its good nutritional and organoleptic qualities and, secondly, for its richness in antioxidants and bioactive compounds. Spain has opted for new tropical crops, and among them, pitahaya is one of the most planted in recent years. Most of the investigations on pitahaya were conducted with red cultivars, while the research on yellow pitahaya (Selenicereus megalanthus Haw.) is very scarce. In this review, the current knowledge about types of pitahayas, the fruit growth and ripening, the quality attributes, the postharvest storage, the use of by-products, and the health attributes were covered.

Biological Functions and Health Benefits of Flavonoids in Fruits and Vegetables: A Contemporary Review.

Flavonoids, being prevalent in fruits and vegetables, are essential to the diverse stages of plant growth, development, and storage. Furthermore, flavonoids have been shown to exert substantial beneficial effects on human health, prompting heightened scientific interest in their potential advantages. This review elucidates the functions of flavonoids in fruits and vegetables, confirming their position as natural sources of these compounds, despite the differences in type and concentration among various species. This review elucidates the significance of flavonoids in the growth and development of fruits and vegetables, highlighting their roles in enhancing pigmentation and providing protection against both biotic and abiotic stresses. In relation to human health, flavonoids are recognized for their ability to combat aging, mitigate inflammation, safeguard the nervous system, and promote overall well-being. Additionally, this review proposes avenues for future research in the domain of flavonoids, underscoring the necessity for ongoing exploration of their potential applications and benefits.

Effect of integrated nutrient management on growth and yield of dragon fruit (Hylocereus polyrhizus Britton & Rose)

Effect of integrated nutrient management on growth and yield of dragon fruit (Hylocereus polyrhizus Britton & Rose)

Calcium application regulates fruit cracking by cross-linking of fruit peel pectin during young fruit growth stage of citrus

Calcium application regulates fruit cracking by cross-linking of fruit peel pectin during young fruit growth stage of citrus

Mechanism of Exogenous Silicon in Enhancing Cold Stress Tolerance in Solanum lycopersicum L. Seedlings: Insights from Resistance and Quality Indicators

Environmental stress, notably the exposure to low temperatures during the early developmental stages of seedlings, has been identified as a critical determinant impacting the yield and quality of tomato crops cultivated in greenhouses. Silicon (Si), recognized as a beneficial element, is posited to mitigate the adverse effects of such stress on plant physiology. This study explores whether exogenous Si fertilizer can effectively alleviate the stress of low temperature and cold damage on tomato plant growth, fruit yield, and quality. Tomato plants were placed under low temperature conditions (6 °C at night, daily average temperature 15 °C), with normal temperature conditions as the control (below 16 °C at night, daily average temperature 28 °C), and two different concentrations of nano Si and ionic Si (50 mg·L−1 and 200 mg·L−1) were sprayed on the leaves, with an equivalent amount of deionized water as the control, for a total of 10 treatments. Relevant indexes were measured to investigate the effects of exogenous Si on tomato resistance, yield, and quality under low-temperature stress. The results show that compared with the control treatment, the plant height, stem diameter, and fresh weight of above-ground and underground parts of tomato seedlings decreased significantly by 46.52%, 42.53%, 28.81%, and 28.97%, respectively, after 15 days of low-temperature stress (p < 0.05), and in order to resist low temperature, the activity of antioxidant enzymes and the content of osmotic adjustment substances were up-regulated in seedlings. Ultimately, low-temperature stress inhibited the morphological growth, nutritional quality, and yield of fruits. Both concentrations of Si application can promote the growth and biomass accumulation of tomato plants under low temperature conditions. Moreover, it significantly ameliorated the osmotic adjustment and antioxidant capacity of the plants, thereby alleviating the low-temperature stress. Under low-temperature stress, 50 mg L−1 ionic Si was the most effective for increasing tomato yield per plant, which was significantly increased by 22.44% compared with the control treatment (p < 0.05). Consequently, the study advocates for the application of 50 mg·L−1 ionic Si fertilizer as a strategy to mitigate the impact of low-temperature stress on tomato plants. Furthermore, the use of nano Si fertilizer has been demonstrated to exert a significant influence on enhancing both the yield and quality of tomatoes, with a 50 mg·L−1 concentration of nano Si fertilizer leading to a notable increase in yield by 20.15% under normal temperature conditions (p < 0.05). These findings are intended to furnish a theoretical foundation and practical direction for advancing research aimed at combating the detrimental effects of low-temperature stress in the context of protected vegetable cultivation.

Comprehensive Identification and Expression Profiling of Lipoxygenase Genes in Sweet Cherry During Fruit Development

Lipoxygenase (LOX) is involved in the oxidation of fatty acids in plants and is a ubiquitous oxygenase that plays an important role in the process of plant resistance to adversity. In this study, the LOX gene family in the sweet cherry genome was identified by bioinformatics methods, the chromosomal mapping of different LOX genes was carried out, and the homology alignment and functional domain analysis of the encoded proteins were performed. The results showed that there were nine LOX gene sequences in the sweet cherry LOX gene family, and the subcellular localization was mainly located in the cytoplasm, chloroplast, or plasma membrane, and was concentrated on chromosomes 1, 2, 3, 4, 6, and 8. During the ripening process of sweet cherry fruits, the LOX gene family showed five different expression patterns, the expression peak of different LOX genes reached the peak of expression at a specific development period, all LOX genes jointly promoted the growth and development of fruits, the enzyme activities of LOX in different varieties of early and late ripening cherries exhibited great differences during the development process, and the results of volatile content in the later stages also showed that different varieties of cherries had their specificity. The results of this study provide a theoretical basis for further revealing the specific functions of LOX gene family members in sweet cherry.

Chemical Topping and Planting Density Interactively Affect Cotton Growth and Partitioning Index

Due to the labor shortage, replacing manual topping with chemical topping has become an important practice in cotton-producing areas such as Xinjiang, China. Similarly, increasing planting density is a common strategy to enhance cotton yields. This study aimed to quantify the interactive effects of chemical topping and planting density on cotton growth and assimilate partitioning. To achieve this, a two-year (2020–2021) field experiment was conducted in Xinjiang, China. The main plots were assigned low (15 plants m−2) or high plant density (25 plants m−2), and the subplots were chemical topping using heavy amounts of mepiquat chloride (MC) or manual topping. The dynamics of dry matter accumulation and partitioning in cotton were well quantified using a beta-growth function with an overall nRMSE of 11.5%. Results indicated that chemical topping increased aboveground dry matter maximum by 4.88%, fruit dry matter maximum by 17.9% and seed cotton yield by 14.0% compared to manual topping. At low plant density, the partitioning index between vegetative and reproductive organs showed no significant differences. However, at high plant density, the time when 50% of assimilate partitioning to reproductive organs for chemical topping was 2.6 physiological days (under optimal temperature conditions) delayed than that for manual topping, resulting in a 2.7 physiological days delay in the time that fruits reached maximum growth rate. The results of the study suggest that applying chemicals for topping a little earlier in high-density planting could reduce the negative effects of delayed boll growth and promote carbon assimilate partitioning to fruit growth.

Training System Effect on Fruit Quality, Yield, Harvest Efficiency, Pruning Times and Growth in Sweet Cherries

Training System Effect on Fruit Quality, Yield, Harvest Efficiency, Pruning Times and Growth in Sweet Cherries

Time-resolved transcriptomic of single V. vinifera fruits: membrane transports as switches of the double sigmoidal growth.

By revealing that the grape berry loses one H+ per accumulated sucrose at the inception of ripening, adopting a single fruit paradigm elucidates the fundamentals of the malate-sugar nexus, previously obscured by asynchrony in population-based models of ripening. More broadly, the development of the individual fruit was revisited from scratch to capture the simultaneous changes in gene expression and metabolic fluxes in a kinetically relevant way from flowering to overripening. Dynamics in water, tartrate, malate, hexoses, and K+ fluxes obtained by combining individual single fruit growth and concentration data allowed to define eleven sub-phases in fruit development, which distributed on a rigorous curve in RNAseq PCA. WGCNA achieved unprecedented time resolutions in exploring transcript level-metabolic rate associations. A comprehensive set of membrane transporters was found specifically expressed during the first growth phase related to vacuolar over-acidification. Unlike in slightly more acidic citrus, H+ V-PPase transcripts were predominantly expressed, followed by V-ATPase, clarifying the thermodynamic limit beyond which their replacement by the tonoplast P3A/P3B ATPase (PH5/PH1) complex turns compulsory. Puzzlingly, bona fide aluminum-activated malate transporter (ALMT) kept a low profile at this stage, possibly replaced by a predominating uncharacterized anion channel. At the onset of ripening, the switch role of hexose transporter HT6 in sugar accumulation is confirmed, electroneutralized by malate vacuolar leakage and H+ pumps activation.

Fruit Growth and Oil Accumulation of Olive (Olea europaea L.) Cultivars at Different Locations Along a Latitudinal–Altitudinal Gradient

Environmental conditions during olive fruit development and genotype have shown to be important sources of variability in final fruit weight and oil concentration. There are fewer studies concerning how the environment and genotype modulate growth dynamics. Thus, the present study aimed to evaluate the timing of fruit growth, pit hardening, and oil accumulation in several olive cultivars under different air temperatures along a latitudinal gradient (29°–33° S) with a range of altitudes (450–1250 masl) in Western Argentina; determine parameters derived from the dynamics of fruit growth and oil accumulation along the same gradient; and explore possible relationships between derived parameters and air temperatures. The cultivars evaluated were Arbequina, Arbosana, Coratina, Changlot, and Picual. Pit hardening occurred 20 days earlier in warmer locations, leading to an earlier onset of oil synthesis. The final fruit dry weight was mainly influenced by the cultivar, which was explained by differences in fruit growth rates. Cooler locations had 39% higher average final fruit dry weight and 22% higher oil concentration than warmer ones. Differences in final oil concentration between genotypes × environments were primarily associated with the oil accumulation rate rather than the duration of the oil accumulation period. However, changes in this rate seem to be cultivar-dependent. Interestingly, the rate and oil accumulation duration showed a negative relationship. These results highlight the need to explore genetic variability in temperature response to limit the detrimental impacts of global warming on olive fruit growth and oil concentration.

Management of Cocoa Plant Pests & Diseases in East Luwu Regency

East Luwu Regency is one of South Sulawesi's regencies with potential in the plantation sector, especially cocoa plants, with a production of 12,250 tons/year. Cocoa productivity has yet to be achieved optimally due to the lack of care and maintenance carried out by farmers, which increases the intensity of pest & disease attacks. This study evaluates farmers' knowledge, attitudes, and actions in managing cocoa plants and plant pathogens in East Luwu Regency. This study was conducted through direct interviews using a structured questionnaire sent to 50 farmers in Burau and Wotu Districts, East Luwu Regency. Respondents were selected purposively, considering that they were easy to find and the location of the plantation was easy to reach. Data are presented in tabular, graphical, and descriptive forms in Microsoft Excel to explain the relationship between farmers' knowledge, attitudes, and actions in managing cocoa VSD disease. The results showed that most of the respondent were active farmer groups. Pests & diseases are problems farmers face in cocoa cultivation. Most farmers used the MCC 02 clone, and the planting distance was 3´3 m. The diseases most commonly found in farmers' cocoa fields include VSD, fruit rot, upas fungus, and stem cancer. Pest & disease control was done through chemical pesticides and pruning (cultivation techniques). Cocoa farmers in Burau and Wotu Districts routinely carry out pruning to control disease in cocoa fields. Farmers prune cocoa plants to remove diseased branches and stimulate the growth of flowers and fruit. Keywords: action, attitude, knowledge, questionnaire

Unraveling water relations in growing fruit: insights from the epidermal growth regulation hypothesis.

This review focuses on the intricate water relationships between internal and external tissues in growing fruits within the framework of the epidermal growth control hypothesis. It considers the components of water potential, including turgor pressure and osmotic potential of both internal and external tissues, taking into account factors such as fruit growth rate, sugar accumulation, cell wall metabolism, and climacteric. It also examines the effects of environmental conditions, genetic factors, and physiological influences in modifying water relations. By emphasizing the significance of skin tissue water potential components as indicators of growth stress, the review underlines their importance for a comprehensive understanding of water relations and associated physiological disorders in growing fruit.

A tomato ethylene-resistant mutant displays altered growth and higher β-carotene levels in fruit

The mutants resistant to ethylene are helpful in deciphering the role of ethylene in plant development. We isolated an ethylene-resistant tomato (Solanum lycopersicum) mutant by screening for acetylene-resistant (atr-1) seedlings. The atr-1 mutant displayed resistance to kinetin, suggesting attenuation of the ethylene sensing response. atr-1 also exhibited resistance to ABA- and glucose-mediated inhibition of seed germination. Unlike the Never-ripe (Nr) mutant seedlings that were hypersensitive to glucose, atr-1 seedlings were resistant to glucose, indicating ethylene sensing in atr-1 is compromised in a manner distinct from Nr. Metabolically, atr-1 seedlings had lower levels of amino acids but higher levels of several phytohormones, including ABA. atr-1 plants grew faster and produced more flowers, leading to a higher fruit set. However, the atr-1 fruits took a longer duration to reach the red-ripe (RR) stage. The ripened atr-1 fruits retained high β-carotene and lycopene levels post-RR stage and had longer on-vine longevity. The metabolome profiles of post-RR stage atr-1 fruits revealed increased levels of sugars. The atr-1 had a P279L mutation in the GAF domain of the ETR4, a key ethylene receptor regulating tomato ripening. The atr-1 exhibits phenotypic traits distinct from the Sletr4-1 (G154S) mutant, thus represents a new ETR4 allele named Sletr4-2. Our study highlights that novel alleles in ethylene receptors may aid in enhancing the nutritional quality of tomato.

The effect of spraying Basfoliar Kelp SL and calcium on some physiological and physical characteristics of date palm fruits, Phoenix dactylifera L, Al-Shwaithi cultivar

This study was conducted during the 2023 growing season in one of the private orchards in Thi Qar Governorate, with the aim of studying the effect of spraying fruits with concentrations of Basfoliar Kelp SL (0, 2 and 4) ml L-1 and calcium (0, 2.5 and 5) ml L-1 and their interactions in Some physiological and physical characteristics of date palm fruits, Al-Shwaithi variety, at the Rubab stage. The experiment was carried out as a factorial experiment according to a completely randomized block design with five replications to calculate the studied characteristics. Concentrations of Basfoliar Kelp SL and calcium were sprayed 21 days after pollination and upon completion of fruit set, and 6 sprays between one another (15 days) and until fruit growth was completed and the coloring phase began. The results can be summarized as follows. The concentration of seaweed extract Basfoliar Kelp SL (4 ml L-1) showed a significant increase in most of the physiological and physical traits studied, and the highest rates were recorded for: maturity rate (87.68%), fruit length (3.76 cm), fruit diameter (2.41 cm), and fruit weight. (10.38 cm) and shoot weight (8.28 kg), in addition to the concentration (4 ml L-1) recorded the lowest rates, with a significant difference in the percentage of fruit drop (12.94%). The calcium concentration (5 ml L-1) showed a significant increase in most of the physiological and physical traits studied, and the highest rates were recorded in the maturity rate (86.38%), fruit length (3.68 cm), fruit diameter (2.38 cm), fruit weight (10.15 g), and bunch weight ( 7.61 kg) for the Rubab stage, in addition to recording the lowest rates with a significant difference in the percentage of fruit drop (10.66%). As for the effect of the interaction parameters between Basfoliar Kelp SL (4 ml L-1) and Calcium (5 ml L-1), it was significant in giving the highest rates of physiological and physical characteristics during the retag stage.

Thermal stability of stigmasterol and β-sitosterol glucosides in fresh-cut bitter melon fruit.

Charantin in M. charantia fruit comprises stigmasterol glucoside (STG) and β-sitosterol glucoside (BSG). Despite numerous studies on charantin quantification and health beneficial bioactivity, thermal stability of two components remains unclear. This study investigated the thermal stability of BSG and STG in the fruit growth stages (S1-S5). Freeze-dried fruit contained approximately twice higher BSG than STG, with the content tending to decrease during fruit maturation. A dramatic decrease in content was observed between S2 and S3, with a reduction of 70% for BSG and 75% for STG. In thermal processing, BSG significantly degraded above 30°C treatments, while STG remained stable. It was found that the thresholds of thermal stability were approximately 10°C for BSG and 30°C for STG. Therefore, minimal thermal processing or freeze-drying is recommended for preserving charantin in the fruit.

Effect of Calcium Sources and Their Mode of Application on Growth and Plant Nutrient Uptake of Two Apple Varieties Grown under High Density Planting System

Apples grown in Kashmir province are famous globally, but lack of post-harvest storage facilities deteriorate its quality and market acceptability. Calcium deficiency in acid soils deceives apple fruit quality. Calcium helps to reduce the occurrence of physiological disorders; it also plays essential role to improve growth and quality of produce. Modes of calcium application and its different doses on two apple varieties was tested in 3 factorial randomized block design to improve yield and quality at Ambri Apple Research Centre, Sher-e-Kashmir University of Agricultural Sciences and Technology, Kashmir, India in 2022. Foliar application of calcium to apple is most effective method for escalating fruit calcium content and quality. Among sources and their mode of application calcium nitrate foliar application at the rate of 3, 4 and 5 gram per litre of water performed better than foliar application of calcium chloride at the rate of 3, 4 and 5 gram per litre of water in improving fruit nutrients and yield of Golden Delicious than Red Chief Camspur, whereas soil application of calcium nitrate showed least response to improve fruit nutrient and yield in both varieties. Fruit length and fruit diameter improved with each enhanced calcium concentration in both varieties by different calcium sources and their mode of application. The partitioning of nutrients in plant leaves and fruits differed significantly, and in both varieties were dependent on applied calcium rates, calcium sources and their mode of application. Calcium nitrate foliar spray performed better than calcium chloride foliar spray or soil application of calcium nitrate in improving fruit growth and quality. Our study did not find any adversity of dosage in plants whether applied as foliar or to soil. The best results were observed in foliar application of calcium nitrate @ 5 g per lit water for all the parameters in both the varieties. Results of research evidenced that preharvest calcium sprays increase growth and improved fruit quality that led to reduce deterioration in post-harvest storage.