Fruit Content Research Articles

Nutritional quality of diet characterized by the Nutri-Score profiling system and cardiovascular disease risk: a prospective study in 7 European countries

Nutri-Score is a scientifically validated 5-color front-of-pack nutrition label reflecting the nutrient profile of foods. It has been implemented in several European countries on a voluntary basis, pending the revision of the European labeling regulation. Hence, scientific evidence is needed regarding the ability of the nutrient profile underlying the Nutri-Score (uNS-NPS, 2023-updated version) to characterize healthier foods. Our objective was therefore to study the prospective association between the nutritional quality of diet characterized by the uNS-NPS and the risk of cardiovascular diseases in a large European population. Our analyses included 345,533 participants from the European Prospective Investigation into Cancer and Nutrition study (EPIC, 1992-2010, 7 European countries). Food intakes were assessed at baseline using country-specific dietary questionnaires. The uNS-NPS was calculated as a continuous scale for each food, based on its 100g content of energy, sugars, saturated fatty acids, salt, fibre, and protein and percentage content of fruit, vegetables, and pulses. A dietary index was derived at the individual level (uNS-NPS DI: energy-weighted mean of uNS-NPS scores of all foods consumed by a participant). Cardiovascular events during follow-up were retrieved using country-specific methods (self-report, registry data). Multi-adjusted Cox models were computed. Overall, 16,214 first cardiovascular events were reported (median follow-up: 12.3 years; 4,103,133 person-years). The consumption of foods with a higher uNS-NPS score (reflecting a lower overall nutritional quality of diet) was associated with higher risks of total cardiovascular events (Hazards Ratio (HR) for an increment of 1 standard deviation: 1.03 (95% Confidence Interval 1.01-1.05)), especially myocardial infarction (HR=1.03 (1.01-1.07)), and stroke (HR=1.04 (1.01-1.07)). In this large prospective study among European adults, a higher risk of cardiovascular diseases (total and several subtypes) was observed in individuals consuming a diet with a lower nutritional value, as graded by the uNS-NPS score. This brings new evidence on the relevance of the updated nutrient profile underlying the Nutri-Score to characterize foods with a healthier nutrient profile. EPIC-CVD was supported by EU FP7, ERC, UK MRC, British Heart Foundation, and UK NIHR.

An ultra-dense linkage map identified quantitative trait loci corresponding to fruit quality- and size-related traits in red goji berry.

Goji berries are a small-fruited shrub with industrial importance whose fruit considered beneficial in both fresh and dried forms. Current germplasms of goji berries include small fruits with a short shelf life, less sweet and bitter taste, and a lack of appropriate genetic information. This study aimed to employ whole genome resequencing to generate an ultra-dense bin linkage map and to elucidate the genetic basis of goji fruit quality and size using quantitative trait loci (QTL) mapping analysis in a cross-pollinated hybrid population. To achieve this goal, human sensory tests were carried out to determine the bitter taste (BT) and sweet taste (ST), and to quantify the soluble solid content (SSC), fruit firmness (FF), and fruit size-related traits of fresh goji fruits over three or four years. The results revealed that the goji bin linkage map based on resequencing spanned a total length of 966.42 cM and an average bin interval of 0.03 cM. Subsequent variant calling and ordering resulted in 3,058 bins containing 35,331 polymorphic markers across 12 chromosomes. A total of 99 QTLs, with individual loci in different environments explaining a phenotypic variance of 1.21-16.95% were identified for the studied traits. Ten major effects, including colocalized QTLs corresponding to different traits, were identified on chromosomes 1, 3, 5, 6, 7, and 8, with a maximum Logarithm of Odds (LOD) of 29.25 and 16.95% of explained phenotypic variance (PVE). In addition, four stable loci, one for FF, one for fruit weight (FW), and two for fruit shape index (FSI), were mainly mapped on chromosomes 5, 6, and 7, elucidating 2.10-16.95% PVE. These findings offer valuable insights into the genetic architecture of goji fruit traits along with identified specific loci and markers to further improve and develop sweeter, less bitter and larger fruited goji berry cultivars with extended shelf life.

Hydrogen sulfide attenuates chilling injury in loquat fruit by alleviating oxidative stress and maintaining cell membrane integrity

The effects of hydrogen sulfide (H2S) on chilling injury (CI), reactive oxygen species (ROS) metabolism, sugar metabolism, pentose phosphate pathway (PPP), and membrane lipid metabolism in loquat fruit throughout the refrigerated period were investigated in this study. The findings indicated that H2S application restrained the increase in internal browning (IB), malondialdehyde (MDA) content, and electrolyte leakage, while sustaining higher total phenolic and total flavonoid levels, and lower soluble quinone content in loquat fruit. Besides, H2S promoted antioxidant accumulation and increased antioxidant enzyme activities by the regulation of ROS metabolism, along with increasing fructose and glucose levels and reducing power by activating sugar metabolism and PPP. Furthermore, H2S treatment retarded the degradation of phospholipids and fatty acids in loquat fruit by modulating membrane lipid metabolism relevant enzyme activities. These findings indicated that H2S application mitigated CI in loquat fruit by alleviating oxidative stress and maintaining cell membrane structural integrity.

Nutritional Changes in stored Tomatoes (Solanum lycopersicum L.) as influenced by Silver Nanoparticles Coating

This study evaluated the influence of silver nanoparticle (AgNP) coating on the nutritional content of stored tomato fruits. Silver nanoparticles was fabricated via the green method of synthesis using ethanolic extract of Moringa oleifera leaves as reducing, capping and stabilizing agent. The antimicrobial efficacy was evaluated at various concentrations (10 – 100 ppm) against Aspergillus niger, Aspergillus paraciticus, Aspergillus flavus and Fusarium oxysporum. Silver nanoparticle coating was prepared from a mixture of silver nanoparticles, carboxyl methyl cellulose (CMC) and sodium caseinate with glycerol as plasticizer.

Safe and Healthy Food Preservation: Efficacy of Guava Leaves and Lemon Juice Extract on Fruits and Vegetables

Background: Fruits and vegetables are naturally perishable and have a relatively limited shelf life. This study was designed to prolong the shelf life and remain nutritional value of various fruits and vegetables by using guava leaves extract and lemon juice extract (natural preservatives). Methods: Fruits and vegetables samples (amla, banana, grapes, bean, eggplant and tomato) were treated with three types of natural preservatives: guava leaves and lemon juice extract (GLE) 0.5% (boiled), lemon extract (LE) 15%, GLE 40% + LE 15%. Results: The study revealed that the moisture content varied from 18.03% to 95.41%. During the 10 days of experiment, samples treated with 0.5% GLE (boiled) preservatives exhibited better result than other two types of preservatives regarding the moisture content. Titratable acidity content varied from 0.05% to 2.25%. The pH value varied in the range of 2.47-6.01. The percentage of weight loss value ranged from 3.67 to 85.71. During the experiment period, weight loss was minimum almost in all samples treated with GLE 40%+LE 15% preservative. The vitamin C content in fruits and vegetables ranged from 1.60 mg/100mL to 68.46mg/100mL. The highest viable count was found in banana (46×104 CFU/ml) and the lowest was in Bean (9×104 CFU/ml). Conclusion: Among three types of preservatives, samples treated with 0.5% GLE (boiled) showed better result and minimum microbial growth.

Effects of cadmium stress on the growth, physiology, mineral uptake, cadmium accumulation and fruit quality of 'Sharpblue' blueberry

Cadmium (Cd) contamination is becoming a widespread environmental problem, and Cd toxicity risk is increasing in blueberries, typical acidophilous plants. However, studies on the physiological mechanisms of Cd toxicity affecting the growth of blueberry plants and fruits remain limited. Herein, a pot experiment involving four treatments, CK (0 mg·kg−1), Cd1 (2.5 mg·kg−1), Cd2 (15 mg·kg−1) and Cd3 (50 mg·kg−1), was conducted to investigate the effects of Cd stress on the growth, fruit quality, physiological characteristics, mineral composition and Cd uptake of 'Sharpblue' blueberry cuttings, and relationships between physicochemical indices and phenotypic data under Cd stress were analyzed via principal component analysis and Mantel's test. Compared with CK, Cd treatment led to chlorosis (decrease in SPAD and N content) in 'Sharpblue' leaves and inhibited increases in plant height, basal stem, crown width and biomass (dry weight), with the most significant changes in the 50 mg·kg−1 Cd treatment. The size and thickness of the upper epidermal cells, as well as the thickness of the palisade mesophyll and spongy tissues of 'Sharpblue' were significantly reduced at Cd > 15 mg·kg−1, while the density of stomata was significantly increased. Moreover, Cd stress reduced the yield (decrease in size and single-fruit weight) and quality (decrease in anthocyanin, flavonoid and total phenol content and increase in titratable acid content) of 'Sharpblue' fruits, and Cd content in fruit exceeded the Chinese food safety threshold of 0.05 mg·kg−1 when Cd > 15 mg·kg−1. The Cd enrichment capacity of different organs of 'Sharpblue' plants was in the following order: root > shoot > leaf > fruit, and the 50 mg·kg−1 Cd treatment significantly inhibited the uptake of Fe, Mn, Cu, and Zn. Additionally, 'Sharpblue' plants responded to H2O2 and malondialdehyde accumulation in different organs under Cd stress by regulating levels of osmoregulatory substances (soluble protein (SP), soluble sugar, and proline) and altering the activities of catalase, superoxide dismutase, ascorbic acid, and glutathione in the antioxidant system. Finally, correlation analysis revealed that Mg, Fe, Mn, Cu, Cd, H2O2, SP, SPAD, N, anthocyanin, titratable acid, flavonoids, and total phenols were strongly correlated with 'Sharpblue' growth, leaf and fruit phenotype under Cd stress. These findings provide theoretical information for soil safety management and the breeding of Cd-tolerant cultivars during blueberry cultivation, as well as for the study of Cd stress response mechanisms in acidophilous plants.

MdWRKY31-MdNAC7 regulatory network: orchestrating fruit softening by modulating cell wall-modifying enzyme MdXTH2 in response to ethylene signalling.

Softening in fruit adversely impacts their edible quality and commercial value, leading to substantial economic losses during fruit ripening, long-term storage, long-distance transportation, and marketing. As the apple fruit demonstrates climacteric respiration, its firmness decreases with increasing ethylene release rate during fruit ripening and postharvest storage. However, the molecular mechanisms underlying ethylene-mediated regulation of fruit softening in apple remain poorly understood. In this study, we identified a WRKY transcription factor (TF) MdWRKY31, which is repressed by ethylene treatment. Using transgenic approaches, we found that overexpression of MdWRKY31 delays softening by negatively regulating xyloglucan endotransglucosylase/hydrolases 2 (MdXTH2) expression. Yeast one-hybrid (Y1H), electrophoretic mobility shift (EMSA), and dual-luciferase assays further suggested that MdWRKY31 directly binds to the MdXTH2 promoter via a W-box element and represses its transcription. Transient overexpression of ethylene-induced MdNAC7, a NAC TF, in apple fruit promoted softening by decreasing cellulose content and increasing water-soluble pectin content in fruit. MdNAC7 interacted with MdWRKY31 to form a protein complex, and their interaction decreased the transcriptional repression of MdWRKY31 on MdXTH2. Furthermore, MdNAC7 does not directly regulate MdXTH2 expression, but the protein complex formed with MdWRKY31 hinders MdWRKY31 from binding to the promoter of MdXTH2. Our findings underscore the significance of the regulatory complex NAC7-WRKY31 in ethylene-responsive signalling, connecting the ethylene signal to XTH2 expression to promote fruit softening. This sheds light on the intricate mechanisms governing apple fruit firmness and opens avenues for enhancing fruit quality and reducing economic losses associated with softening.

Determination of the Protein and Amino Acid Content of Fruit, Vegetables and Starchy Roots for Use in Inherited Metabolic Disorders.

Amino acid (AA)-related inherited metabolic disorders (IMDs) and urea cycle disorders (UCDs) require strict dietary management including foods low in protein such as fruits, vegetables and starchy roots. Despite this recommendation, there are limited data on the AA content of many of these foods. The aim of this study is to describe an analysis of the protein and AA content of a range of fruits, vegetables and starchy roots, specifically focusing on amino acids (AAs) relevant to AA-related IMDs such as phenylalanine (Phe), methionine (Met), leucine (Leu), lysine (Lys) and tyrosine (Tyr). AA analysis was performed using high-performance liquid chromatography (HPLC) on 165 food samples. Protein analysis was also carried out using the Dumas method. Foods were classified as either 'Fruits', 'Dried fruits', 'Cruciferous vegetables', 'Legumes', 'Other vegetables' or 'Starchy roots'. 'Dried fruits' and 'Legumes' had the highest median values of protein, while 'Fruits' and 'Cruciferous vegetables' contained the lowest median results. 'Legumes' contained the highest and 'Fruits' had the lowest median values for all five AAs. Variations were seen in AA content for individual foods. The results presented in this study provide useful data on the protein and AA content of fruits, vegetables and starchy roots which can be used in clinical practice. This further expansion of the current literature will help to improve diet quality and metabolic control among individuals with AA-related IMDs and UCDs.

Modification of Fungicide Treatment Needs and Antioxidant Content as a Result of Real-Time Ozonation of Raspberry Plants.

Raspberry plants need intensive anti-fungal protection. A solution to this problem could be the application of an ozonation process. For this purpose, a technical solution was proposed and implemented in raspberry plant production. The proposal suggests replacing 25% of standard fungicide treatments with ozonation. It was demonstrated that the use of ozone under the proposed conditions made it possible to maintain stable parameters of chlorophyll content and fluorescence (no significant differences), but the intensity of gas exchange was increased. The greatest differences were observed in the second measurement period (T2), when the plants were in the stage of most active development. Additionally, the content and profile of low-molecular-weight antioxidants and the microbial load were determined in the collected fruits. In periods T2 and T3, the proposed method caused a reduction reaching ~2 log cfu g-1 in the microbial content of raspberry fruits. It was shown that ozone treatment intensified the biosynthesis of low-molecular-weight antioxidants in fruit (increasing the total polyphenol content by more than 20%). The proposed scheme allows a 25% reduction in standard fungicide treatments while maintaining the health of cultivated raspberry plants. The reduction in fungicide use aligns with the EU regulations and produces fruit with better quality.

Substantiation of wild plants used as functional ingredients in the technology of crisp grain bread

Research on the nutritional content of wild plant fruits in Kazakhstan, including Hawthorn fruit (Crataegus laevigata), rosehip fruit (Rose canina L. variety), sea buckthorn fruit (Hippophae rhamnoides - Altai variety), and black chokeberry fruit (Aronia melanocarpa), has determined that these fruits possess a combination of properties essential for maintaining human health, attributed to the presence of bioactive substances (BAS) and other components. The technological process for obtaining extracts and concentrates from wild-growing raw material fruit has developed. The following extraction modes were proposed: ultrasonic wave frequency 40 kHz, extraction time 30 minutes, temperature 500С, and concentration of the obtained extracts was carried out by vacuum evaporation method, using IKA RV-10 apparatus at 40-50°С and pressure 800 mbar. Found that extracts and concentrates have a higher concentration of nutritional substances when compared to the fruits themselves. Thus, the vitamin C content in hawthorn fruits was 27.8 mg, while in the extract, it was 47.84 mg, and in the concentrate – 62.19 mg. The vitamin C content in rosehip fruits, extracts, and concentrates was 578.01 mg, 811.8 mg, and 1101.3 mg, respectively. The vitamin C content in sea buckthorn fruits, extracts, and concentrates was 285.05 mg, 518.8 mg, and 640.9 mg, respectively. The vitamin C content in black chokeberry fruits, extracts, and concentrates was 86.2 mg, 128.8 mg, and 160.5 mg, respectively. A similar increase was observed for the content of vitamin E and other components. Furthermore, the obtained concentrates meet the safety parameters required by regulatory documents. A technology for producing grain crispbread using the extrusion method has developed, incorporating whole grains, groats, flavouring additives, and concentrates derived from wild fruits such as hawthorn, rosehip, sea buckthorn, and black chokeberry into the recipe.

Impact of microbial-based biopreparations on soil quality, plant health, and fruit chemistry in raspberry cultivation

Application of microbial-based biopreparations as a pre-harvest strategy offers a method to obtain sustainable agricultural practices and could be an important approach for advancing food science, promoting sustainability, and meeting global food market demands.The impact of a bacterial-fungal biopreparation mixture on soil-plant-microbe interactions, fruit chemical composition and yield of 7 raspberry clones was investigated by examining the structural and functional profiles of microbial communities within leaves, fruits, and soil. Biopreparation addition caused the enhancement of the microbiological utilization of specific compounds, such as d-mannitol, relevant in plant-pathogen interactions and overall plant health. The biopreparation treatment positively affected the nitrogen availability in soil (9–160%).The analysis of plant stress marker enzymes combined with the evaluation of fruit quality and chemical properties highlight changes inducted by the pre-harvest biopreparation application. Chemical analyses highlight biopreparations' role in soil and fruit quality improvement, promoting sustainable agriculture. This effect was dependent on tested clones, showing increase of soluble solid content in fruits, concentration of polyphenols or the sensory quality of the fruits. The results of the next-generation sequencing indicated increase in the effective number of bacterial species after biopreparation treatment. The network analysis showed stimulating effect of biopreparation on microbial communities by enhancing microbial interactions (increasing the number of network edges up to 260%) of and affecting the proportions of mutual relationships between both bacteria and fungi.These findings show the potential of microbial-based biopreparation in enhancing raspberry production whilst promoting sustainable practices and maintaining environmental homeostasis and giving inshght in holistic understanding of microbial-based approaches for advancing food science monitoring.

Glycoside hydrolase PpGH28BG1 modulates benzaldehyde metabolism and enhances fruit aroma and immune responses in peach.

Benzaldehyde (BAld) is one of the most widely distributed volatiles that contributes to flavor and defense in plants. Plants regulate BAld levels through various pathways, including biosynthesis from trans-cinnamic acid (free BAld), release from hydrolysis of glycoside precursors (BAld-H) via multiple enzymatic action steps, and conversion into downstream chemicals. Here, we show that BAld-H content in peach (Prunus persica) fruit is up to 100-fold higher than that of free BAld. By integrating transcriptome, metabolomic and biochemical approaches, we identified glycoside hydrolase PpGH28BG1 as being involved in the production of BAld-H through the hydrolysis of glycoside precursors. Overexpressing and silencing of PpGH28BG1 significantly altered BAld-H content in peach fruit. Transgenic tomatoes heterologously expressing PpGH28BG1 exhibited a decrease in BAld-H content and an increase in SA accumulation, while maintaining fruit weight, pigmentation, and ethylene production. These transgenic tomato fruits displayed enhanced immunity against Botrytis cinerea compared to wild type (WT). Induced expression of PpGH28BG1 and increased SA content were also observed in peach fruit when exposed to Monilinia fructicola infection. Additionally, elevated expression of PpGH28BG1 promoted fruit softening in transgenic tomatoes, resulting in a significantly increased emission of BAld compared to WT. Most untrained taste panelists preferred the transgenic tomatoes over WT fruit. Our study suggests that it is feasible to enhance aroma and immunity in fruit through metabolic engineering of PpGH28BG1 without causing visible changes in the fruit ripening process.

Utilizing RT-DETR Model for Fruit Calorie Estimation from Digital Images

Estimating the calorie content of fruits is critical for weight management and maintaining overall health as well as aiding individuals in making informed dietary choices. Accurate knowledge of fruit calorie content assists in crafting personalized nutrition plans and preventing obesity and associated health issues. In this paper, we investigate the application of deep learning models for estimating the calorie content in fruits from digital images, aiming to provide a more efficient and accurate method for nutritional analysis. We create a dataset comprising images of various fruits and employ random data augmentation techniques during training to enhance model robustness. We utilize the RT-DETR model integrated into the ultralytics framework for implementation and conduct comparative experiments with YOLOv10 on the dataset. Our results show that the RT-DETR model achieved a precision rate of 99.01% and mAP50-95 of 94.45% in fruit detection from digital images, outperforming YOLOv10 in terms of F1- Confidence Curves, P-R curves, precision, and mAP. Conclusively, in this paper, we utilize a transformer architecture to detect fruits and estimate their calorie and nutritional content. The results of the experiments provide a technical reference for more accurately monitoring an individual’s dietary intake by estimating the calorie content of fruits.

The art of tartness: the genetics of organic acid content in fresh fruits.

Organic acids are major determinants of fruit flavor and a primary focus of fruit crop breeding. The accumulation of organic acids is determined by their synthesis, degradation, and transport, all of which are manipulated by sophisticated genetic mechanisms. Constant exploration of the genetic basis of organic acid accumulation, especially through linkage analysis, association analysis, and evolutionary analysis, have identified numerous loci in recent decades. In this review, the genetic loci and genes responsible for malate and citrate contents in fruits are discussed from the genetic perspective. Technologies such as gene transformation and genome editing as well as efficient breeding using marker-assisted selection (MAS) and genomic selection (GS) are expected to break the bottleneck of traditional fruit crop breeding and promote fruit quality improvement.

How Nano-ZnO Affect Tomato Fruits (Solanum lycopersicum L.)? Analysis of Selected Fruit Parameters.

Tomato (Solanum lycopersicum L.), as one of the most valuable horticulture crops, was chosen to investigate the effect of nanoparticles (NPs) in the form of nano-ZnO combined with conventional fertilizer on the quality of tomato fruits, including their antioxidant potential (total antioxidant activity, lycopene and β-carotene content), sugars content and allergenic potential (profilin and Bet v 1 content). Nano-ZnO was implemented during plant cultivation, applied by foliar spraying or directly via soil, at three different concentrations (50, 150 and 250 mg/L). The obtained results suggest that the usage of NPs during tomato plant cultivation had minor impacts on parameters such as total antioxidant activity or the content of selected allergens. Even though the total antioxidant activity was not affected by nano-ZnO, the malondialdehyde activity (MDA) content was notably decreased in fruits under nano-ZnO treatment. The content of lycopene and β-carotene was significantly affected by the use of nano-ZnO. Moreover, the usage of nano-ZnO significantly increased the total sugar content in fruits treated with nanoparticles via foliar spraying. Based on the obtained results, it can be stated that nano-ZnO, regardless of the method of application, significantly affected tomato fruits which can be beneficial for fruit production.

Effect of Different Levels of Farmyard Manure (FYM) Integrated with Organic Modules on Yield, Quality and Pest Incidence of Okra (Abelmoschus esculentus L. Moench)

The study was conducted during late Kharif season during 2021-22 and 2022-23 at administrative office (PGIHS), Sri Konda Laxman Telangana State Horticultural University, Mulugu, Telangana, India. The experiment was laid out in contrast factorial randomized block design (CFRBD) with two factors i.e., factor-1 consists of three levels of FYM and factor-2 consists of three organic modules and separate control (100% RDF) plot was grown and replicated thrice. The pooled results indicated that, control treatment (100 % RDF-100:50:50 kg NPK/ha) reported highest number of fruits per plant (19.05), highest fruit weight (14.72 g), maximum fruit yield per plant (0.28 kg), maximum fruit yield per hectare (9.52 t/ha) over the other treatments. L1M1 (FYM equivalent to 100 % RDN + organic module-1) registered minimum crude fibre content (7.10%), and maximum chlorophyll content in fresh fruit (1.19 mg/100g). While, minimum mean percent of fruit infestation (6.28%) was reported with control (100% RDF-100:50:50kg NPK/ha + emamectin benzoate 5%SG @ 70 g/200 lit) and lowest mean population of jassid per leaf (1.08/leaf), was recorded with control (100 % RDF-100:50:50 kg NPK/ha + imidacloprid 17.8 SL @ 100 ml/acre).

Determination of trace elements content of fruits from Tehran's market using ICP- OES method: a risk assessment study.

For the first time in Iran, in this study, the amount of 19 trace elements in some types of commonly consumed Iranian fruits (in their peel and pulp) was evaluated by ICP-OES (Inductively coupled plasma-optical emission spectrometry) method. Based on the outcomes, the highest and lowest average detected elements in all fruits samples were related to (Al) aluminum (1842.18) and (V) vanadium (0.28) ppm, respectively. Mercury (Hg) and antimony (Sb) were not detected (ND) in any samples. Also, the maximum mean of elements in quince, lemon, grapefruit, kiwi, orange south, orange north and tangerine samples was related to(Fe) iron (2048.32 ppm), (Zn)zinc(753.45 ppm), Fe (1056.33 ppm), Al (9794.41 ppm), Zn (717.78 ppm), Fe (1334.87 ppm) and Fe (974.93 ppm), respectively. Furthermore, our outcomes revealed, the highest mean of elements in kiwi peel, kiwi pulp, orange North peel, orange North pulp, orange South peel, orange South pulp, quince peel, quince pulp, grapefruit peel, grapefruit pulp, lemon peel, lemon pulp, tangerine peel and tangerine pulp was related to Al (17967.79 ppm), Al (1621.03 ppm), Fe (1350.01 ppm), Al (1457.66 ppm), Zn (934.71 ppm), Fe (728.06 ppm), Fe (2768.11 ppm), Fe (1328.54 ppm), Zn (1008.54 ppm), Fe (1198.00 ppm), Zn (683.35 ppm), Zn (823.55 ppm), Fe (1182.59 ppm), and Fe (767.27 ppm), respectively. Based on the Monte Carlo simulation results, the THQ (target hazard quotient) and ILCR (Incremental Lifetime Cancer Risk) related to exposure to heavy metals via fruits for adults and children showed that there is no significant non-carcinogenic risk (THQ < 1) and carcinogenic risk (ILCR < 1E-4) for adults and children.

Phosphate fertilization to improve yield and chemical composition of banana ‘BRS SCS Belluna’ fruit

Phosphate fertilization improves banana yields, but due to the physiological effects of phosphorus on plants, it can also be used to improve the physical and chemical characteristics of the fruits, which can contribute to increasing the processing of green bananas and reducing losses in the production chain. Herein, the productivity of bunches and fruit, the biometric analysis of the fruit, dry matter content, and the chemical composition of immature fruit were evaluated when the cultivar BRS SCS Belluna (AAA) was grown under different levels of phosphate fertilization for two harvest cycles. The results showed that phosphate fertilizer levels interfered with bunch yield and fruit mass in both cycles, leading to yield gains of 44.3% (1st cycle) and 84.0% (2nd cycle) in bunches and 51.8% (1st cycle) and 81.9% (2nd cycle) in fruits. Fertilization promoted gains of up to 33.5% in fruit mass and enhanced the resistant starch content in the pulp, leading to an increase of 15.1%. The phosphorus, potassium, and calcium contents in green fruits increased with fertilizer levels in the first cycle, improving nutritional quality. These results have useful applications because they indicate that adequate management of phosphate fertilization can increase the potential of the cultivar for industrial processing of green fruits and the nutritional quality of the derived products.

Wavelengths Selection Method in Near Infrared Spectra Using Multivariate Analysis for Nondestructive Determination of Oil Content in Palm Oil Fruits

Wavelengths Selection Method in Near Infrared Spectra Using Multivariate Analysis for Nondestructive Determination of Oil Content in Palm Oil Fruits

Metabolic and gene-expression analyses reveal developmental dynamics of cutin deposition in pomegranate fruit grown under different environmental conditions

The chemical and transcriptional changes in the cuticle of pomegranate (Punica granatum L.) fruit grown under different environmental conditions were studied. We collected fruit from three orchards located in different regions in Israel, each with a distinct microclimate. Fruit were collected at six phenological stages, and cutin monomers in the fruit cuticle were profiled by gas chromatography-mass spectrometry (GC–MS), along with qPCR transcript-expression analyses of selected cutin-related genes. While fruit phenotypes were comparable along development in all three orchards, principal component analyses of cutin monomer profiles suggested clear separation between cuticle samples of young green fruit to those of maturing fruit. Moreover, total cutin contents in green fruit were lower in the orchard characterized by a hot and dry climate compared to orchards with moderate temperatures. The variances detected in total cutin contents between orchards corresponded well with the expression patterns of BODYGUARD, a key biosynthetic gene operating in the cutin biosynthetic pathway. Based on our extraction protocols, we found that the cutin polyester that builds the pomegranate fruit cuticle accumulates some levels of gallic acid—the precursor of punicalagin, a well-known potent antioxidant metabolite in pomegranate fruit. The gallic acid was also one of the predominant metabolites contributing to the variability between developmental stages and orchards, and its accumulation levels were opposite to the expression patterns of the UGT73AL1 gene which glycosylates gallic acid to synthesize punicalagin. To the best of our knowledge, this is the first detailed composition of the cutin polyester that forms the pomegranate fruit cuticle.