This study aimed to validate the effectiveness of the Articulation Motion Assessment System (AMAS), a joint kinematic evaluation system, for clinical applications. AMAS enables synchronised measurement using neurophysiological indicators, overcoming laboratory setting limitations. We compared AMAS-based ankle joint kinematic evaluations, particularly the sagittal and frontal plane angles, with two-dimensional (2D) motion analysis to determine the validity and reliability of AMAS. Both AMAS and 2D motion analysis reliably detected significant differences in angles within the sagittal and frontal planes. Correlation analysis revealed a significant moderate-to-strong correlation between the AMAS and the conventional method of 2D motion analysis, proving the measurement validity of the AMAS (ρ = 0.53–0.77 for sagittal plane angles; ρ = 0.46–0.72 for frontal plane angles). The average root mean squared error (RMSE) was significantly lower in AMAS (10.90 ± 2.93° for sagittal plane angles; 13.44 ± 1.09° for frontal plane angles) than in the inertial sensor-based three-dimensional (3D) motion analysis. Reliability analysis revealed high reliability of measurements (intraclass correlation coefficients (ICC) ≥ 0.76). However, the Bland–Altman analysis identified a slightly lower fixed bias, which was observed as a characteristic of each measurement system. The AMAS accurately detects ankle joint angles without being constrained by measurement environment limitations. Synchronised measurements using neurophysiological indicators potentially contribute to understanding ankle joint control mechanisms and developing rehabilitation strategies.
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