Fritillaria Ebeiensis Research Articles

Phylogenetic analyses reveal the Chinese medical plant “Beimu” Fritillaria ebeiensis as a separate species

The Chinese medical plant “Beimu” Fritillaria ebeiensis has been merged in F. anhuiensis. In this study, we used ITS, two plastid (rpl16 and matK) markers, and morphological characters to investigate the relationship between F. ebeiensis and F. anhuiensis. The results show that F. ebeiensis is phylogenetically distinct from F. anhuiensis. In addition, the morphology of both leaf epidermis and bulbs are different between the two species. As a consequence, we treat F. ebeiensis as a separate species, rather than synonymy of F. anhuiensis.

Chemical constituents from Fritillaria anhuiensis

Abstract Phytochemical investigation on Fritillaria anhuiensis led to the isolation of one new labdane diterpenoid ( 12 ) 14( RS ),15-dihydroxy-8(17),12( E )-labdadien-19-oic-acid and 26 known compounds ( 1 – 11 , 13 – 27 ). Their structures were determined on the basis of spectroscopic analysis and by comparison of their spectral data with those reported in the literature. Fourteen known compounds ( 4 , 5 , 7 – 11 , 13 , 14 , 16 , 17 , 22 – 24 ) were isolated from F . anhuiensis for the first time, and five of them ( 10 , 11 , 13 , 23 , 24 ) are reported from the family Liliaceae for the first time. Some of the compounds identified in the present investigation may have chemotaxonomic value at the generic level.

Prokaryotic expression of a Thunberg fritillary mosaic virus CP gene and antiserum preparation

Wei, C. B., Wu, Q. Y., Yang, Y. and Liu, C. Y. 2012. Prokaryotic expression of a Thunberg fritillary mosaic virus CP gene and antiserum preparation. Can. J. Plant Sci. 92: 495–500. Fritillaria anhuiensis is a valued traditional Chinese medicinal plant. Thunberg fritillary mosaic virus (TFMV) was found to cause mosaic symptoms. Virus particles of the Lu'an isolate were purified and its RNA genome was extracted. cDNA was synthesized from the RNA and specific PCR primers were used to amplify the virus coat protein (CP) gene. It shared 96.8% nucleotide identity and 97.6% amino acid identity with the sequences of CPs from the other TFMV isolates. The CP gene was inserted into pSBET and expressed in Escherichia coli BL21 (DE3) plys S strain. The expressed protein was first purified by 12% (wt/vol) SDS-PAGE and subsequently by 5–20% (wt/vol) gradient SDS-PAGE. Antiserum against the CP was raised in mouse and its specificity was confirmed by Western blot analysis suggesting that it may be useful for detecting the...

Prokaryotic expression of a Thunberg fritillary mosaic virus CP gene and antiserum preparation

Wei, C. B., Wu, Q. Y., Yang, Y. and Liu, C. Y. 2012. Prokaryotic expression of a Thunberg fritillary mosaic virus CP gene and antiserum preparation. Can. J. Plant Sci. 92: 495–500. Fritillaria anhuiensis is a valued traditional Chinese medicinal plant. Thunberg fritillary mosaic virus (TFMV) was found to cause mosaic symptoms. Virus particles of the Lu'an isolate were purified and its RNA genome was extracted. cDNA was synthesized from the RNA and specific PCR primers were used to amplify the virus coat protein (CP) gene. It shared 96.8% nucleotide identity and 97.6% amino acid identity with the sequences of CPs from the other TFMV isolates. The CP gene was inserted into pSBET and expressed in Escherichia coli BL21 (DE3) plys S strain. The expressed protein was first purified by 12% (wt/vol) SDS-PAGE and subsequently by 5–20% (wt/vol) gradient SDS-PAGE. Antiserum against the CP was raised in mouse and its specificity was confirmed by Western blot analysis suggesting that it may be useful for detecting the virus.

Genetic diversity and relationship of Fritillaria thunbergii Miq. landraces and related taxa

Abstract Genetic diversity and differentiation of four landraces of Fritillaria thunbergii Miq. and two congeners Fritillaria cirrhosa D. Don and Fritillaria anhuiensis S. C. Chen et S. F. Yin were evaluated using ISSR markers. The results showed that the genetic diversity of F . thunbergii was high at the specie level but relatively lower at the landrace level. A high level of genetic differentiation among four F . thunbergii landraces was detected based on the gene differentiation coefficient and the AMOVA, in line with the low inter-landrace gene flow. MS-tree analysis showed that the four F . thunbergii landraces were clustered on adjacent positions of the tree, and that Kuanye (Ft-KY) landrace was relatively distantly related to other landraces. In line with the MS-tree analysis, PCoA revealed that the three species of Fritillaria can be divided into two groups and three subgroups, among which there occurred a remarkable genetic differentiation.

A novel sulfur-containing diterpenoid from Fritillaria anhuiensis

Two novel diterpenoids were isolated from the bulbs of Fritillaria anhuiensis S. C. Chen and S. E. Yin. Compound 1 was the first diterpenoid containing a sulfonyl group isolated from nature. Compound 2 was a novel kaurane-type diterpenoid. Their structures were determined by extensive spectroscopic analysis (IR, MS, NMR, and X-ray diffraction). Compound 1 significantly attenuated nitric oxide (NO) production of a macrophage cell line of Raw 264.7 cells stimulated with IFN-γ.

ChemInform Abstract: Structures of Three New Diterpenoids, Fritillebic Acid and Fritillebins A and B, from Bulbs of Fritillaria ebeiensis G. D. Yu et G. Q. Ji.

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Active constituents of the bulbs of Fritillaria ebeiensis and their antitumour activity in mice

AbstractThe crude alkaloid of Fritillaria ebeiensis G. D. Yu et G. Q. Ji, administrated orally at a given dose, showed strong antitumour activity in inhibiting the growth of the solid type of hepatoma in mice. Chemical studies revealed that the active principles in this crude drug were verticine, verticinone and ebeiedine obtained from this species for the first time. This paper deals with the isolation and activity of these principles.

Structures of Three New Diterpenoids, Fritillebic Acid and Fritillebins A and B, from Bulbs of Fritillaria ebeiensis G. D. Yu et G. Q. JI.

A new diterpenoid, fritillebic acid (1) and four new diterpenoid dimers, fritillebins A (2) and B (3), and fritillebinides A and B, were isolated from a crude drug, bulds of Fritillaria ebeiensis G. D. Yu et G. Q. JI. The structures of compounds 1-3 were determined to be ent-3β-acetoxy-16β-kauran-17-oic acid (1), ent-16β-hydroxykauran-17-yl ent-3β-acetoxy-16β-kauran-17-oate (2), and ent-3β-acetoxy-16β-hydroxykauran-17-yl ent-3β-acetoxy-16β-kauran-17-oate (3), respectively, on the basis of spectroscopic and chemical evidence.

The structural elucidation of fritillaria alkaloids from Fritillaria ebeiensis var. purpurea. I. The structures of ebeienine, ebeiedine and ebeiedinone.

A novel (22S, 25R)-13, 17-didehydro: 5α-cevanine alkaloid, ebeienine (1), was isolated from the bulbs of Fritillaria ebeiensis var.purpurea, together with (25S)-20-deoxy-5α-cevanin-3, β, 6β-diol (ebeiedine (2)), and (25S)-20-deoxy-5α-cevanin-3α-o1-6-one (ebeiedinone (3)). Their structures were elucidated by physical methods and finally confirmed by X-ray crystal structure determination.