This research has developed a methodology to estimate energy losses associated with auxiliary systems and mechanisms subject to friction in a stationary diesel engine. The study methodology is based on mathematical models used to calculate engine friction forces and power losses. The analysis involves the cooling, the lubrication, the fuel injection system, and the engine's valve train, bearings, and piston mechanism. A stationary diesel engine with four load conditions and three rotational speed conditions has been taken as a reference for the study. The analysis of the results has showed that fuel injection is the auxiliary system with the highest energy consumption. In general, the cooling system, the lubrication, and the injection represent loss energy equivalent to 0.3%, 0.5%, and 0.9% of the chemical energy of the fuel. The interaction between the cylinder liner and the piston skirt is the main source of energy loss associated with friction processes. For valve train mechanisms, bearings and pistons it has been evidenced that these represent 1.25%, 1.91%, and 5.26% of the chemical energy of the fuel. In general, the methodology proposed in this research is a tool that allows diagnosing energy losses in auxiliary systems and engine mechanisms, which is useful for evaluating future strategies focused on energy improvement.