Freshwater Shrimp Research Articles

Infection with Clonorchis sinensis (Cobbold, 1875) Metacercariae in Fish from the East Lake of Wuhan: Freshwater Fish in Urban Lakes May Act as Infection Sources of Liver Fluke.

The liver fluke disease caused by Clonorchis sinensis is one of the most serious food-borne parasitic diseases in China. Many freshwater fish and shrimps can be infected with C. sinensis metacercariae as the second intermediate hosts in endemic regions. Owing to the lack of infected humans and the good administration of pet dogs and cats in cities of non-endemic regions, few fish are expected to be infected with C. sinensis metacercariae in urban lakes. To determine the infection of C. sinensis metacercariae in freshwater fish and shrimps in urban lakes, a total of 18 fish species and one shrimp species were investigated in the East Lake of Wuhan City. Metacercariae were isolated by artificial digestive juice and identified using morphology and rDNA-ITS2 sequences. Five species of fish, Pseudorasbora parva, Ctenogobius giurinus, Squalidus argentatus, Hemiculter leuciclus, and Rhodeus spp., were infected with C. sinensis metacercariae. The overall prevalence of C. sinensis was 32.5%. The highest prevalence was found in P. parva with 57.9%, while S. argentatus exhibited the highest mean abundance (13.9). Apart from the C. sinensis metacercariae, four species of other trematode metacercariae were also identified across twelve fish species in total. Owing to the consumption of undercooked fish and feeding cats with small fish caught by anglers, there is a potential risk that the small fish infected with C. sinensis metacercariae may act as an infection source to spread liver fluke. Given the complete life cycle of C. sinensis, stray cats and rats were inferred to act as the important final hosts of C. sinensis in urban lakes in non-endemic areas.

Development and testing of a sedation protocol for Neocaridina davidi

Neocaridina davidi, a small freshwater shrimp native to Asia, specifically China, Japan, Korea, and Vietnam, possesses remarkable resistance to poor water quality and offers various advantages over other invertebrate species to examine crucial issues in neuroscience and other related areas. These advantages include robustness, ease of maintenance, and transparency, making them useful for in vivo studies with optical imaging techniques. Despite its suitability for research purposes, particularly in the fields of imaging and fluorescent techniques, the lack of attention given to this species has resulted in the absence of a robust and replicable sedation protocol for immobilization and safe manipulation. Consequently, researchers face challenges in performing experimental procedures while minimizing harm to this specimen. In this study, we have developed and evaluated a simple sedation protocol specifically designed for Neocaridina davidi, assessing its effectiveness using light microscopy and image processing.

Use of black soldier fly larvae and freshwater shrimp to partly substitute commercial diet for Nile tilapia cultured in smallholder fish farms – A case study in Busia County, Kenya

Fish-farming in Kenya is challenged by the availability and high cost of feed ingredients, especially protein sources. Using black soldier fly larvae (Hermetia illucens) (BSFL) directly or indirectly as a feed ingredient to feed fish is interesting as they efficiently convert organic wastes into high-quality proteins. In addition, the freshwater shrimp (Caridina nilotica) (CN), an important by-catch of the silver cyprinid (Rastrineobola argentea) fisheries in Lake Victoria, could be another alternative protein source. Therefore, this study determined whether dried BSFL or dried CN could partly substitute the commercial diet when growing Nile tilapia (Oreochromis niloticus) in a smallholder farm aggregated in an aquapark. The nutritional values and cost-effectiveness of the alternative feeding strategies were compared to commercial diet (CD) only. During an 84-day experimental period, Nile tilapia were fed one of the three feeding strategies, including the use of only the commercial diet, to be compared with diets replacing 20% of the commercial diet with BSFL or CN. The fish were fed to apparent satiation twice a day. At the end of the trial, biomass, the number of fish, growth, survival rate, total fish production, economic feed conversion rate and productivity per pond, were determined. As such, Body Weight (BW) was similar across feeding strategies (P > 0.05), measured 63.2 ± 3.9, 68.0 ± 7.4 and 68.0 ± 7.4 g for fish-fed diets with CD, BSFL and CN, respectively. Hence, nutritionally and from a production point of view, the three feeding strategies used in this study performed equally well. Notably, the use of BSFL led to a significantly higher economic return (P < 0.05) compared to the use of CN and commercial diet alone. The trials in this study demonstrate that BSFL and CN can be used to partly substitute commercial diet. In addition, the trial show that the use of BSFL to partly substitute the commercial diet can boost sustainable fish production in a smallholder farm set-up aggregated in an aquapark.

Field and laboratory microplastics uptake by a freshwater shrimp.

Microplastics are widespread pollutants, but few studies have linked field prevalence in organisms to laboratory uptakes. Aquatic filter feeders may be particularly susceptible to microplastic uptake, with the potential for trophic transfer to higher levels, including humans. Here, we surveyed microplastics from a model freshwater shrimp, common caraidina (Caridina nilotica) inhabiting the Crocodile River in South Africa to better understand microplastic uptake rates per individual. We then use functional response analysis (feeding rate as a function of resource density) to quantify uptake rates by shrimps in the laboratory. We found that microplastics were widespread in C. nilotica, with no significant differences in microplastic abundances among sampled sites under varying land uses, with an average abundance of 6.2 particles per individual. The vast majority of microplastics found was fibres (86.1%). Shrimp microplastic accumulation patterns were slightly higher in the laboratory than the field, where shrimp exhibited a hyperbolic Type II functional response model under varying exposure concentrations. Maximum feeding rates of 20 particles were found over a 6 h feeding period, and uptake evidenced at even the lowest laboratory concentrations (~10 particles per mL). These results highlight that microplastic uptake is widespread in field populations and partly density dependent, with field concentrations corroborating uptake rates recorded in the laboratory. Further research is required to elucidate trophic transfer from these taxa and to understand potential physiological impacts.

Integrated analysis of transcriptomic and physiological responses to cold stress in Macrobrachium rosenbergii

The giant freshwater prawn (GFP), Macrobrachium rosenbergii, stands out as a vital species in freshwater shrimp aquaculture. However, its susceptibility to cold conditions has increased the cost of aquaculture and limited the growth of GFP cultivation. To furnish insights at the molecular level to enhance GFP's cold tolerance, transcriptome sequencing and physiological analysis of the hepatopancreas were performed in our study. Initially, we identified 4909 differentially expressed genes (DEGs) between experimental (15 °C, 24 h) and control groups (25 °C, 24 h). These DEGs were significantly enriched in the proteasome, spliceosome, lysosome, peroxisome, and lipid metabolism-related pathways including glycerolipid metabolism, sphingolipid metabolism, linoleic acid metabolism as well as steroid hormone biosynthesis. Histological examination following various stress durations revealed a notable increase in hepatocyte vacuoles after 6 h of cold stress. Hepatocyte structure deterioration occurred in the later phase (48 h), coinciding with a significant increase in cell apoptosis. Subsequent analyses demonstrated an initial increase followed by a decrease in superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) content, while malonaldehyde (MDA) content rose with stress duration. Furthermore, a significant increase in triglyceride content and no significant change in cholesterol content was observed. RT-qPCR results unveiled significant down-regulated expression of FAS, TAGL, ACOX1, ACS and significant up-regulated expression of GPAT and DEGS. These findings suggest that oxidative stress and impaired lipid metabolism underlie the poor cold tolerance of GFP.

Three New Species of the Freshwater Shrimp Genus Caridina from Australia

Three new species of the genus Caridina are described from the northernmost part of Australia. Caridina darwin n. sp. resembles Caridina temasek Choy and Ng, 1991 but differs in the armature of the rostrum, the development of epipods on the pereiopods and the absence of an appendix interna on the male first pleopods. Caridina magnovis n. sp. resembles Caridina serratirostris de Man, 1892 but differs in the armature of the ventral margin of the rostrum, a shorter stylocerite, a stouter carpus of the first pereiopod, the number and size of spiniform setae on the third and fifth pereiopods, the shape of the preanal carina and the size of the embryos (referred to as “eggs” in most previous publications). Caridina wilsoni n. sp. resembles Caridina gracilirostris de Man, 1892 but differs in the size of the embryos and in some length to width ratios of the segments of the pereiopods. Detailed morphological descriptions of all three new species are given. A molecular phylogeny (mt DNA 16S) supports the morphospecies hypothesis and illustrates the phylogenetic relationship with morphologically similar species from outside Australia.

Ultra-performance liquid chromatography-tandem mass spectrometry for quantification of pyrethroid pesticide residues in freshwater fish and shrimp

A sophisticated Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS) approach was devised for the concurrent quantification of pyrethroid pesticide residues in freshwater fish and shrimp. This analytical method involved ultrasonic extraction using acetonitrile, succeeded by sample purification employing Primary Secondary Amine (PSA) and Zirconium Dioxide Silica (Z-Sep+) following low-temperature centrifugation. Chromatographic separation was achieved with an ACQUITY UPLC BEH C18 column utilizing a mobile phase of a 2 mmol/L ammonium formate aqueous solution and methanol, with detection conducted through ESI + ionization in Multiple Reaction Monitoring (MRM) mode. The assay demonstrated exceptional linearity for seven pyrethroids across a concentration range of 0.5–10 μg/L, evidenced by correlation coefficients surpassing 0.999. Detection limits were established between 0.08 and 0.52 μg/kg, and the lower limits of quantification ranged from 0.28 to 1.72 μg/kg. Furthermore, recovery rates for blank samples were outstanding, ranging from 91.40% to 108.83% at concentrations of 1.0, 4.0, and 20.0 μg/kg, with Relative Standard Deviations (RSDs) between 0.94% and 8.13%. This methodology proved to be rapid, convenient, sensitive, and robust, offering stable recovery rates. Notably, it facilitated the detection of seven pyrethroid pesticide residues in freshwater fish and shrimp within an expedited timeframe of 15 min. This pioneering technique constitutes a significant advancement in the development of new methods for ensuring food safety.

Feasibility analysis of the neocaridina ornamental shrimp cultivation business in Gunung Mulya Village, Tenjolaya District, Bogor Regency

Currently, fish farming activities are carried out not only to meet people's consumption needs, but many farmers also use the waters to cultivate ornamental shrimp, one of which is the freshwater ornamental shrimp type Neocaridina. This research was conducted in Gunung Mulya Village, Tenjolaya District, Bogor Regency. This research aims to determine innovation and technical cultivation as well as the feasibility of cultivating freshwater ornamental shrimp of the Neocaridina type in Gunung Mulya Village, Tenjolaya District, Bogor Regency, which includes identifying costs, revenues, profits, and business feasibility. The research method used in data collection is quantitative methods to identify costs, revenues, profits, and business feasibility, which aim to determine whether or not the freshwater ornamental shrimp cultivation business in Gunung Mulya village, Tenjolaya subdistrict, Bogor Regency, is feasible to run. Based on the results of the data analysis, it was concluded that cultivating freshwater ornamental shrimp of the Neocaridina type in Gunung Mulya village, Tenjolaya subdistrict, Bogor Regency, was profitable, with the total profit obtained in 1 production cycle being IDR. 15,413,334. The financial aspects of business feasibility indicators obtained show an R/C ratio of 2.60 and a B/C ratio of 1.60. Thus, the freshwater ornamental shrimp cultivation business in Gunung Mulya Village, Tenjolaya subdistrict, Bogor Regency, is feasible. This research provides insight into the techniques and innovations used in cultivating Neocaridina ornamental shrimp.

Individual and combined impact of microplastics and lead acetate on the freshwater shrimp (Caridina fossarum): Biochemical effects and physiological responses

Microplastics and heavy metals pollution is recognised as a major problem affecting aquatic ecosystems. For this reason, this study aims to assess the toxicity of different concentrations of polyethylene microplastics (PE-MPs) (0.0, 500, and 1000 μg L−1) with a mean size of 15–25 μm and lead acetate Pb(C2H3O2)2 (0.0, 2.5, and 5 mg L−1), both individually and in combination, through the exposure of the freshwater grass shrimp, Caridinia fossarum for 15 days, focusing on microplastic interaction with co-occurring contaminants. After being exposed to both contaminants, either individually or in combination, significant alterations in numerous biochemical markers were observed. Specifically, exposure to lead acetate alone resulted in significant changes across ALP, AST, ALT, LDH, GGT, and BChE enzyme activity levels indicating hepatotoxicity and neurotoxicity. Also, Pb exposure led to alterations in total antioxidant capacity, MDA, total lipids, and glycogen contents, signalling the onset of oxidative stress. Exposure to PE-MPs alone led to changes in ALP, LDH, GGT, and BChE enzyme levels, and in MDA, total lipids, and glycogen samples' contents. Remarkably, the study observed increased bioaccumulation of lead acetate in samples treated with the combination, emphasizing the synergistic impact of PE-MPs on the toxicity of lead acetate. This synergy was also evident in AST and ALT enzyme activity levels and MDA contents. This underscores the necessity for measures to address both microplastic pollution and heavy metal contamination, taking into account the synergistic behaviour of MPs in the presence of concurrent contaminants.

Outcomes of double-blind, placebo-controlled food challenges with seawater and freshwater shrimps in Chinese patients with adverse food reactions to shrimp

Outcomes of double-blind, placebo-controlled food challenges with seawater and freshwater shrimps in Chinese patients with adverse food reactions to shrimp

Toxic effects of nickel on tolerance and regeneration in the freshwater shrimp Neocaridina davidi

Heavy metals cause environmental pollution and produce toxic effects on organisms. Nickel (Ni) is a common metallic pollutant of aquatic ecosystems and potentially can produce multifarious changes in the body of aquatic organisms. The average nickel content in rivers is about 0.7 μg/l. As a result, the homeostasis of the affected organism is disturbed, and processes that can counteract the changes are activated. To better understand the effects of Ni in the freshwater environment and its fauna, it is important to establish whether all changes caused in cells and tissues by Ni exposure are reversible when the animal returns to the non-polluted environment. Thus, the main aim of the study was to analyze changes that occur after Ni exposure and after it is returned to non-contaminated water at various levels of the animal’s body. The freshwater shrimp Neocaridina davidi (Crustacea) was selected as the subject of the study. As the organ for studies, we chose the midgut because it is a barrier against stressors that enter the organism. A concentration of 3.63 mg Ni/l was selected for the experiment, at which approximately 50% mortality of the population was observed after 14 days. The midgut was analyzed using light and transmission electron microscopy (TEM), confocal microscopy, and flow cytometry for qualitative and quantitative results. When the organisms were transferred to clean water, a prolong exposure resulted in a decrease in the values of the analyzed parameters (e.g. ROS, cell death, etc.) proportional to the purification time. The recovery time was insufficient to return to control values in most analyzed groups. Nevertheless, the occurrence of regenerative changes suggests that freshwater shrimps are relatively tolerant to nickel, when the exposure time is short and the recovery time is long.

Metal concentrations (Fe and Zn) in Macrobrachium amazonicum from Brazilian Amazon rivers

The objective of this work was to analyze the contamination by iron (Fe) and zinc (Zn) in the shrimp Macrobrachcium amazonicum in the region of the mouth of the Amazon River in the State of Amapá, taking into account aquaculture and fishing activities. 90 shrimp were used, 45 females and 45 males of M. amazonicum. The animals (30) were found in three different points, being Mazagão Velho in Mazagão, a community on Ilha de Santana, a district in the Brazilian municipality of Santana and Arquipélago do Bailique. Fe and Zn determinations were performed by conventional flame atomic absorption spectrophotometry. The greatest accumulation of Zn in the organs and the lowest concentration of Zn in the muscle and exoskeleton of M. amazonicum was observed. Fe had the highest accumulation in organs, followed by exoskeleton and muscle. Zn accumulation in male organs was greater than in muscle and exoskeleton while Fe concentrations were greater in organs, followed by exoskeleton and muscle. In females, Zn accumulation was greater in our organs, with no difference between muscle and exoskeleton. According to the present study, to maintain levels of food security, the consumption of freshwater shrimp caught and farmed in Mazagão, Bailique Archipelago and community of Ilha de Santana should be limited to a daily shrimp intake dose of 96. 52 g for Fe and 226.81 g for Zn.

Genetic structure of Macrobrachium nipponense, an important farmed freshwater shrimp in China, in the Three Gorges Reservoir

Macrobrachium nipponense is a major farmed species freshwater shrimp in China and its genetic diversity is of high value in aquaculture. The construction of the Three Gorges Project has slowed down the water velocity in the Three Gorges Reservoir (TGR) and made the water more clear, which is more favourable for the survival and reproduction of M. nipponense. However, there are lack of studies on the genetic diversity of M. nipponense populations in the TGR. In this study, mitochondrial COI gene sequences were used as molecular marker to analyze the genetic diversity, genetic differentiation and historical demography of eight M. nipponense populations in the TGR. The results showed that the M. nipponense populations in TGR have high genetic diversity. There was some genetic differentiation between the four populations in lower reaches of the TGR and four populations in the upper reaches of the TGR. We also found that there was a significant correlation between genetic distance and geographical distance among the M. nipponense populations in the TGR. The M. nipponense population in the TGR has experienced population reduction in the recent year, which might be related to the ice age movement in the Little Ice Age and human activities. This study provides a theoretical basis for the rational development and protection of M. nipponense resources in the TGR.

Appearance of exotic shrimp Palaemon sinensis (Sollaud, 1911) and other freshwater shrimps before and after the 2018 extreme flood in western Japan

Appearance of exotic shrimp Palaemon sinensis (Sollaud, 1911) and other freshwater shrimps before and after the 2018 extreme flood in western Japan

INVASI UDANG AIR TAWAR Macrobrachium lanchesteri DI DANAU BOLANO TOGA, TOLI-TOLI, SULAWESI TENGAH

Sulawesi Island has long been known as one of the regions in Indonesia that holds a wealth of freshwater shrimp species in both lentic and lotic waters. One of the lentic waters in Sulawesi is Lake Bolano Tago which is located in Toli-Toli Regency, Central Sulawesi. This research aims to reveal the population and abundance of freshwater shrimp in the lake. Sampling was carried out in April 2022 along the lake's littoral zone which was divided into 7 stations using purposive sampling and sweep methods. Sampling effort at each station was carried out for 4 hours. Environmental physicochemical factors measured include water temperature, brightness, dissolved oxygen levels, and water acidity levels. Specimen identification was carried out at the Animal Biosystematics and Evolution Laboratory, Biology Department, FMIPA, Tadulako University. Identification was carried out based on morphological characters by following the identification key of Wowor et al (2004). The results of this research revealed that of the freshwater shrimp collected as many as 1,165 individuals in the lake, all consisted of one species, namely Macrobrachium lanchesteri. This species belonging to the Palaemonidae family is not native to Indonesia but comes from the Oriental region. Macrobrachium lanchesteri was first described based on a type specimen originating from Thailand. Its presence in Lake Bolano Toga is quite worrying because of its invasive nature. With the abundant presence of the M. lanchesteri species at all stations, it can be confirmed that the Macrobrachium lanchesteri species has invaded Lake Bolano Toga

Effects of two diets on growth and condition factor of Chitala lopis

The giant featherback Chitala lopis is fully protected fish species according to Indonesia's official list. The conservation of aquatic resources is achieved through implementing fish farming practices and optimizing feed utilization. This study aims to investigate the impact of different feeding rate on the growth patterns, performance, and condition factors of the first generation of giant featherback. The first generation of the fish were collected from the natural reproduction of broodstock in the earthen ponds. Fish were randomly divided at three different groups replicated three times and reared in the nine net cages installed in the earthen pond. The stocking density of the fish in each group was three fish per cage. The feed treatment involved fish bycatch as control (T0) (5% of the total weight) and freshwater shrimp, Caridina sp. with two different feeding rates including 5% (T1) and 7% (T2) of the total weight, respectively. The mean initial weight of the fish was 41 ± 3.85 g. The survival and absolute growth rate of T1 were highest (29.9±6.38 g, 78± 11%), but it is not significantly different (p0.05). The average relative growth rate of T1 is also highest (76.28±18.61%) and significantly different (p0.05). The growth pattern of the fish at the beginning of the study was negative allometric (0.54), which was low correlated (0.03), and at the end became positive allometric (4.17), which was moderately correlated (0.66). The highest condition factor was T2 (0.78±0.03) and had a significant effect (p0.05). Caridina sp. has a good impact on the first generation of giant featherback weight growth compared to other treatments in this study.Keywords:CultivationFeeding rateGiant featherbackCaridina sp.Rasbora sp.

Effects of different feeding rate on growth and condition factor of Chitala lopis

Chitala lopis, also known as giant featherback, is a fully protected fish species on Indonesian official list due to the susceptibility to exploitation. Several studies showed that the conservation of aquatic resources, such as giant featherback, could be achieved through the implementation of fish farming practices and optimization of feed use. Therefore, this study aimed to determine effect of different feeding rate on growth pattern, performance, and condition factor of the first generation of giant featherback. The first generation of fish was collected from the natural reproduction of broodstock in the earthen ponds. A total of 5 samples were then randomly divided into three treatment groups and reared in the nine net cages installed in the earthen pond. The stocking density in each group was three fish per cage, and the treatments were carried out in triplicates. Feed given comprised fish bycatch as control (T0) (5% of the total weight) and freshwater shrimp, Caridina sp. with two different feeding rates, namely 5% (T1) and 7% (T2) of the total weight. The mean initial weight of all the samples in this study was 41 ± 3.85 g. The results showed that the highest survival and absolute growth rates (29.9±6.38 g, 78± 11%) were obtained in T1, but were not significantly different (p0.05). Furthermore, the highest average relative growth rate was also found in T1 (76.28±18.61%) and significantly different compared to other treatments (p0.05). Growth pattern of fish at the beginning of the experiment was negative allometric (0.54), showing a low correlation (0.03), but became positive allometric (4.17) at the end, with moderate correlation (0.66). The results also showed that the highest condition factor was obtained in T2 (0.78±0.03), and there was a significant effect (p0.05). Based on the results, Caridina sp. had a positive and significant impact on the weight growth of the first generation of giant featherback compared to other studies.Keywords:CultivationFeeding rateGiant featherbackCaridina sp.Rasbora sp.

Cloning, Identification, and Functional Analysis of the Foxl2 Gene in Procambarus clarkii.

Procambarus clarkii is the most widely distributed freshwater shrimp in China, with important economic value and great potential for development. The forkheadboxL2 (Foxl2) gene has been found to be involved in the reproductive development of many crustaceans. To understand the role of the Foxl2 gene in the gonad development of P. clarkii, we designed CDS-specific primers for the P. clarkii Foxl2 (PcFoxl2) gene and cloned its CDS sequence using RT-PCR. The nucleotide and protein sequence information was then analyzed through bioinformatics analysis. The expression and subcellular localization of PcFoxl2 in various tissues were detected using qRT-PCR and in situ hybridization. The effects of PcFoxl2 knockdown on gonad development were investigated using RNA interference. The results showed that the CDS length of the PcFoxl2 gene was 1614 bp and encoded 537 amino acids. Protein sequence comparison and phylogenetic analysis showed that PcFoxl2 was the closest relative to Crayfish. qRT-PCR analysis indicated that the expression level of PcFoxl2 in the testis was significantly higher (>40 fold) than that in the ovary (p < 0.01). The in situ hybridization results showed that PcFoxl2 was expressed in both the cytoplasm and the nucleus of egg cells, and that the expression was strongest in egg cells at the early stage of yolk synthesis, while weak in the secondary oocytes. The positive signal was strongest in the spermatocyte nucleolus, while only a trace signal was observed in the cytoplasm. After interfering with the PcFoxl2 gene using dsRNA, the expression of PcFoxl2 in the RNA interference group was significantly lower than that in the control group, and this interference effect lasted for one week. Moreover, the gonad index of the experimental group was significantly lower than that of the control group (p < 0.05) after 10 days of P. clarkii cultivation following PcFoxl2 knockdown. The expression levels of the nanos and S3a genes, which are related to gonad development, decreased significantly after PcFoxl2 gene interference. The results suggest that the Foxl2 gene is involved in the growth and development of gonads, particularly in the development of testis, and is related to the early development of oocytes. This study provides a theoretical basis for the artificial breeding of P. clarkii.

The Potential of Whole Soya Bean Meal in Raising Nile Tilapia (Oreochromis Niloticus, L.) Fingerlings

An eight-week experimental study was undertaken to determine the effect of whole soya bean meal (SBM) with reference to common fish feed ingredients, consisting of Omena (Rastineobola argentea) meal (RAM), Freshwater shrimp (Caridina niloticus) meal (CNM) and commercial fish meal (CFM, control diet) on water quality, growth performance and survival of O. niloticus fingerlings.

Updated Checklist of the Freshwater Shrimps (Decapoda: Caridea: Atyidae) of Mindoro Island, the Philippines, with a Description of a New Species of Caridina

Following recent expeditions to Mindoro Island, the Philippines, numerous samples of atyid shrimps were collected and then studied in an integrative taxonomy framework. A total of 16 species belonging to 4 genera are hereby reported, including 8 new records for the island and/or the Philippines: Atydina atyoides, Caridina bruneiana, C. celebensis, C. elongapoda, C. papuana, C. parvirostris, C. typus, C. zhujiangensis, and one new species, Caridina leptopoda sp. nov. Sequences of 16S rRNA have been produced for all of the species and taxonomical notes are provided. To account for the morphological variability across its range, C. bruneiana Choy, 1992 is herein re-described based on specimens from Mindoro. All of the species reported here from Mindoro have small eggs and are considered amphidromous, which suggests that they all potentially occur in other localities. This contrasts with other islands of the Philippines such as Bohol or Luzon where endemic species with large eggs can be found.