Freshwater Contamination Research Articles

Plastic in the Environment: A Modern Type of Abiotic Stress for Plant Physiology.

In recent years, plastic pollution has become a growing environmental concern: more than 350 million tons of plastic material are produced annually. Although many efforts have been made to recycle waste, a significant proportion of these plastics contaminate and accumulate in the environment. A central point in plastic pollution is demonstrated by the evidence that plastic objects gradually and continuously split up into smaller pieces, thus producing subtle and invisible pollution caused by microplastics (MP) and nanoplastics (NP). The small dimensions of these particles allow for the diffusion of these contaminants in farmlands, forest, freshwater, and oceans worldwide, posing serious menaces to human, animal, and plant health. The uptake of MPs and NPs into plant cells seriously affects plant growth, development, and photosynthesis, finally limiting crop yields and endangering natural environmental biodiversity. Furthermore, nano- and microplastics-once adsorbed by plants-can easily enter the food chain, being highly toxic to animals and humans. This review addresses the impacts of MP and NP particles on plants in the terrestrial environment. In particular, we provide an overview here of the detrimental effects of photosynthetic injuries, oxidative stress, ROS production, and protein damage triggered by MN and NP in higher plants and, more specifically, in crops. The possible damage at the physiological and environmental levels is discussed.

Infiltration of freshwater food chain by nanoplastics: An examination of trophic transfer and biological impact

The contamination of freshwater by nanoplastics (NPs) poses a significant threat to various organisms. However, the influence of food factors on the NPs toxicity and the associated risks to water safety remains poorly understood. In this study, we employed highly fluorescent NPs incorporating aggregation-induced emission fluorogens to quantify and visualize the interactions of NPs with freshwater phytoplanktonic alga Chlamydomonas reinhardtii and zooplankton Daphnia magna, including the uptake of NPs by algae and daphnids, as well as gut environment responses through both waterborne and foodborne routes. NPs at environmentally relevant concentrations were capable of aggregating and internalizing in planktonic algae, thus permeating the food chain and potentially causing ecological risks. Notably, positively charged NPs exhibited greater infiltration than the negatively charged NPs. Our findings indicated that NPs had a greater tendency to accumulate in Daphnia via the food chain rather than through direct exposure to water. This was attributed to the loss of selectivity, suggesting a potential mechanism for NP accumulation in aquatic food chains. However, daphnids exposed to NPs through waterborne route exhibited more gut pH acidification, esterase activity, and gut inflammation compared to those exposed via the foodborne route. Moreover, chronic exposure to NPs through waterborne routes significantly disrupted the growth, molting, and reproduction of Daphnia compared to the foodborne group. These findings provided valuable insights into the complexities of plastic pollution on primary trophic levels in aquatic environments.

Distribution of Heavy Metals in Water and Bottom Sediments in the Basin of Lake Gusinoe (Russia): Ecological Risk Assessment

Fresh water scarcity is considered a significant component, and potentially one of the most critical, of global climate change. With the rapid development of industry, there is an increasing risk of freshwater contamination by heavy metals (HMs). The danger of HM pollution is also attributed to their accumulation, which can subsequently become a source of secondary pollution in aquatic environments. In the Lake Gusinoe basin, located in Russia, concentrations of HMs were measured in both water and bottom sediments within the lake area, as well as in inflowing and outflowing watercourses. Ecological risk indices were also calculated for the Gusinoe basin. Our results showed that the average concentrations of Fe, Zn, Cr, Ni, Cd, and Pb in the water did not exceed the maximum allowable concentrations (MACs) set by Russian national standards and WHO standards, while the concentrations of Mn and Cu exceeded the corresponding MACs during winter, spring, and autumn possibly due to decomposition of aquatic vegetation and influx from groundwater sources. The average concentrations of the investigated HMs in the BSs did not exceed the background values. The water hazard index indicated a low risk for all samples in the lake water area. For all BS samples, the geoaccumulation index (Igeo) and the Pollution Load Index (PLI) indicated low pollution levels, while the values of the Enrichment Factor (EF) and the Contamination Factor (CF) indicated moderate pollution in the central part of the lake. The Ecological Risk Factor (Er) for Cu in BSs at points near major settlements and in the Tel River indicated moderate pollution. The Potential Ecological Risk Index (RI) in all investigated BS samples indicated a low risk of contamination.

Bacterial community responses of the hydrothermal vent crab Xenograpsus testudinatus fed on microplastics

Microplastics (MPs) provide persistent contaminants in freshwaters and the oceans from anthropogenic sources worldwide. MP contamination in ecosystems has emerged as a global environmental issue. While increasing research focused on the ecological consequences of plastic pollution, health-related implications of plastic pollution have been somewhat overlooked. In this study, we evaluated the effects of polyethylene MP contamination on microbial, physical, and biochemical characteristics of the hydrothermal vent crab Xenograpsus testudinatus over a 7-day food exposure. Different concentrations (0%, 0.3%, 0.6%, and 1.0%) of polyethylene MPs were used for feed intake experiments. Oxford Nanopore Technologies’ full-length sequencing of the 16S rDNA gene was used to explore the changes of the microbial composition in vent crab tissues. At the phylum level, the content of Firmicutes significantly decreased in the digestive gland tissue. Furthermore, the predicted functions of genes in the microbial community were significantly influenced by MPs. In contrast, there were eight functions in gill and 11 functions in digestive gland tissues identified at low and high intake levels. The dominant functions of methylotrophy, dark thiosulfate oxidation, dark oxidation of sulfur compounds, aromatic hydrocarbon degradation, and aromatic compound degradation were significantly increased at high intake levels in the digestive gland. These findings indicate that MP ingestion causes not only a short-term decrease in energy intake for crustaceans but also a change in microbial communities and their functions. This study provided a first account on the toxicity of MPs in a hydrothermal vent crab to aid in the assessment of health risks provided by polyethylene MP to marine invertebrates.

Searching for Balance between Hill Country Pastoral Farming and Nature

Much land has been cleared of indigenous forest for pastoral agriculture worldwide. In New Zealand, the clearance of indigenous forest on hill country has resulted in high food production, but waterways have become turbid, with high nutrient and E. coli concentrations. A range of on-farm mitigations are available, but it is unclear how they should be applied catchment-wide. We have developed a catchment-scale model that integrates economics with ecosystem services to find a better balance between agriculture and nature. In the upper Wairua catchment, Northland, if three actions are prioritised—(1) keeping stock out of streams, (2) constructing flood retention bunds in first-order catchments, and (3) planting trees on highly erodible land—then sediment loads, E. coli levels, and flooding are significantly reduced. Implementing these actions would cost approximately 10% of catchment net revenue, so it is feasible with a combination of regulation and subsidy. Many catchments in New Zealand are primarily pastoral agriculture, as in other countries (in North and South America, Australasia, and the United Kingdom), and would benefit from the analysis presented here to guide development along sustainable pathways. While pastoral agriculture typically stresses waterways, with increased sedimentation and freshwater contaminants, much can be done to mitigate these effects with improved farm and riparian management.

Assessing coastal vulnerability and land use to sea level rise in Jeddah province, Kingdom of Saudi Arabia

Sea level rise is one of the most serious outcomes of increasing temperatures, leading to coastal flooding, beach erosion, freshwater contamination, loss of coastal habitats, increased soil salinity, and risk of damage to coastal infrastructures. This study estimates the vulnerability to inundation for 2100 in coastal zones in Jeddah Province, Kingdom of Saudi Arabia, under various sea level rise (SLR) scenarios of 1, 2, 5, and 10 m. The predicted flooding was estimated using a combination of factors, including SLR, the bathtub model, digital elevation model, climate scenarios, and land use and land cover. The climate scenarios used were Representative Concentration Pathway (RCP) scenarios 1.9, 2.6, 4.5, and 8.5. The results of the SLR scenarios of 1, 2, 5, and 10 m revealed that 1.6, 4.7, 14.9, and 30.6% (or 88, 214, 679, 1398 km2) of the study area's coast could be classified as inundated areas. The various SLR scenarios can inundate 3.3 to 34% of the road area/length. The inundated built-up and road areas were estimated to range between 0.31 and 0.79 km2, accounting respectively for 1.18 to 3.01% of the total class areas for 1-meter and 2-meter SLR scenarios. In contrast, the inundated area will be significant in the situation of 5 and 10 m SLR scenarios. Regarding the case of a 10-meter SLR scenario, the inundation will negatively impact the built-up and road infrastructure areas, inundating 8.9 km2, with industrial infrastructures affected by inundation estimated at 0.21 km2, followed by green space infrastructures at 0.013 km2. The spatial information based on various SLR scenario impact mapping for Jeddah Province can be highly valuable for decision-makers to better plan future civil engineering structures within the framework of sustainable development.

Accumulation of metal(loid)s in the digestive gland of the mussel Unio crassus Philipsson, 1788: A reliable detection of historical freshwater contamination

The possible influence of historical contamination of water/sediments on the metal(loid) bioaccumulation in the digestive gland of mussel Unio crassus Philipsson, 1788, from two differently contaminated sites at the Mrežnica River was studied in three seasons. The first data for this species on total/cytosolic concentrations of 27 (non)essential elements were obtained by HR ICP-MS. Higher bioaccumulation was observed at the historically contaminated site, with several nonessential elements (Bi, Cs, Pb, Sb, Tl, U) found in 5–6 times higher concentrations compared to the reference site. Although both total and cytosolic levels revealed the influence of water/sediment contamination, the latter showed association between bioaccumulation and exposure for larger number of studied elements. At the reference site, several elements (Ba, Ca, Cd, Cr, Mn, Sr) were also found in 2–10 times higher concentrations compared to contaminated one, but it was attributed to background levels characteristic for karst rivers (for Ca and Cd), and to coaccumulation due to chemical similarity (for Ba, Cr, Mn, Sr). The seasonal variability was also observed, with generally highest metal(loid) concentrations in mussel digestive glands found in autumn which was associated to mussels reproductive period. Our results confirmed that sediment-dwelling mussels, specifically U. crassus, represent a good bioindicators for detection of historical pollution due to their direct contact/exposure to contaminants stored in sediments, with concurrent consideration of physiological/chemical factors. Historical contamination potentially can have serious impact on freshwater environment even long time after its cessation, and, therefore, a careful continuous monitoring is recommended.

A Case Study on Bacteriological and Physiochemical Evaluation of Hospitals Drinking Water in Pakistan

Background: Seventy percent of the Earth is covered by water, out of which only 1% is fresh water. This fresh water can be used for drinking and other domestic uses. However, a drastic increase in the industrial revolution resulted in depletion of the reservoirs and contamination of the potable fresh water. Around 3.4 M deaths per annum occur as a result of waterborne diseases. Objectives: Therefore, the aim of this study is to establish physical, chemical, and biological parameters for evaluating contamination in the drinking water of hospitals in the Bannu and Kohat divisions, Khyber Pakhtunkhwa, Pakistan. Method: Eleven different hospitals were selected for the collection of water samples. Total solids, total dissolved solids, electrical conductivity, and pH were considered significant physical metrics for this study. The essential and heavy metals were also quantified. Furthermore, biological parameters such as Escherichia coli (E. coli) content of drinking water were also studied by using membrane filtration. Results: The results show that E. coli were detected in nine out of the eleven water samples. Moreover, some of the physicochemical parameters were not within guideline limits specified by the World Health and other international organizations. Conclusions: E coli in most of the hospital drinking water was more than the WHO permissible level of the physiochemical parameters. This will have adverse effects on the health of patients which is a serious threat for the population. Therefore, this investigation provides useful information to the government to take special precautions for maintaining the quality of the potable water in government and private hospitals.

Pyridinium-functionalized Ionic Porous Organic Polymer for Rapid Scavenging of Oxoanions from Water.

Metal oxoanions adversely affect the food chain through bioaccumulation and biomagnification. Therefore, they are among the major freshwater contaminants that require immediate remediation. Although several adsorbents have been developed over the years for sequestering these micropollutants, the selective removal of oxoanions remains still a formidable challenge. Herein, pyridinium and triazine-based ionic porous organic polymer, iPOP-Cl, developed through a Brønsted acid-catalyzed aminal formation reaction, has been reported as a suitable anion exchange material for the selective removal of metal oxoanions from wastewater. The positively charged nitrogen centers, along with exchangeable chloride counter-ions in the porous polymer, allow facile oxoanion uptake. iPOP-Cl is found to be a selective scavenger of permanganate (MnO4 - ) and dichromate (Cr2 O7 2- ) from water in the presence of a high concentration of competing anions generally found in brackish water. The material exhibits fast sorption kinetics, a high uptake capacity (333mg g-1 for MnO4 - and 358mg g-1 for Cr2 O7 2- ), and excellent recyclability. This article is protected by copyright. All rights reserved.

Repeated applications of farm dairy effluent treated with poly-ferric sulphate did not adversely affect soil phosphorus availability, P fractions and pasture response — a 4-year field plot study

PurposeLand application of farm dairy effluent (FDE) can cause phosphorus contamination of freshwater due to its high nutrient content especially phosphorus (P) in the animal dung. A novel FDE treatment technology has been developed that uses poly-ferric sulphate (PFS) to treat the FDE and recycle water for washing farmyard and reduce the risk of water pollution from P leaching from through the soil. It is important that the application of PFS-treated FDE (TE) does not cause any adverse impacts on soil fertility or plant growth when the TE is applied to the soil.Materials and methodsA multi-year field plot study was conducted to determine the effect of repeat applications of FDE and PFS-treated FDE (TE) on soil P availability, P fractionations, plant yield and nutrient uptake. Eight applications of untreated FDE, TE and water as control were applied to replicated soil plots over the period of 4 years. The soil samples were collected on 1 December 2020, and nine pasture samples were harvested during the 2021–2022 dairy milking season. Measurements included soil chemical properties, soil phosphorous fractionations, plant biomass and plant phosphorus and nitrogen uptake.Results and discussionThe results indicated that the majority of soil fertility indices and soil P fractions had no significant difference between the FDE and TE applications, with the exception of labile P which was significantly higher in the TE (122.7 mg kg−1) than in the FDE treatments (103.0 mg kg−1) at 0–10-cm soil depth and was also significantly higher in the TE (114.6 mg kg−1) than in the FDE treatments (74.0 mg kg−1) at 10–20-cm soil depth. Similarly, plant P uptakes and dry matter yields were also the same between the TE and FDE treatments with the average of being 54.4 kg P ha−1 and 12.8 t ha−1, respectively.ConclusionsRepeated applications of PFS-treated FDE had no adverse effect on soil P availability or plant growth when compared to untreated FDE application and had the potential to benefit soil fertility compared to control.

Soil management effects of extensive pastures, intensive pastures and sugarcane crops on the availability of metals and nutrients in freshwater: A realistic mesocosm approach

Changes in land use have led to important modifications in aquatic ecosystems around the world. In Brazil, extensive pastures are being converted to sugarcane crops to avoid deforestation, while the remaining pastures are being intensified to compensate for the loss of area. In this study, the hypothesis that conventional soil management practices conducted on intensive pasture and sugarcane crops increase the availability of contaminants in freshwater was tested. Thirteen mesocosms embedded in extensive pasture (EP), intensive pasture (IP), and sugarcane crops (C) systems were monitored for 392 days. The monitoring included the measurement of in situ parameters (pH, dissolved oxygen, electrical conductivity, turbidity, and chlorophyll), nutrients (total nitrogen, nitrite, nitrate, ammonium ion, total phosphorus, total dissolved phosphorus, inorganic phosphorus, and silicate), and metals (aluminum, cadmium, copper, manganese, nickel, lead, potassium, and zinc). Soil management practices were performed in IP and C treatments at full-scale (tillage, application of fertilizers, vinasse and pesticides, and cattle pasture). The patterns observed for each treatment were obtained developing Principal Component Analysis over time. The mesocosms in the EP treatment did not experience large variations in water quality over time since no soil management was performed in this treatment. However, we found that fertilizer and vinasse application was the major precursor to the deterioration of water quality over time. After the first fertilizer application, we found eight and 16 times higher concentrations of total phosphorus, and seven and 45 times higher concentrations of total nitrogen in the IP and C treatments, respectively, in comparison to their concentration in the EP treatment. The application of vinasse in sugarcane crop increased water conductivity, potassium, and manganese concentrations in mesocosms. Dissolved oxygen and pH values decreased over time in all treatments. Overall, soil management practices resulted in greater deterioration of water quality in the sugarcane treatment than in the intensive pasture.

Development of a New Method to Estimate the Water Purification Efficiency of Bulk-Supported Nanosorbents under Realistic Conditions

The direct use of nanosorbents for water purification is limited due to their aggregation and the lack of techniques for their recovery from natural waters. To overcome these problems, the affixation of nanomaterials onto bulk, non-mobile supports has been proposed. However, a method to simulate the efficiency of these sorbents under realistic conditions is still not available. To address this need, this work describes a method for evaluating the sorption efficiency of nanosorbent materials incorporated on bulk supports under non-equilibrium conditions. The method combines the principles of passive sampling, an environmental monitoring technique that is based on passive diffusion of dissolved contaminants from water to a sorbent, with batch sorption experiments that measure sorption under equilibrium conditions, to determine the parameters associated with water purification. These parameters are the maximum sorption capacity of the sorbent and the sampling rate, which is the volume of contaminated water treated per unit of time. From these variables, the deployment time of the sorbent until reaching saturation is proposed as an alternative indicator of sorbent efficiency. As proof-of-principle, the removal of oxyanions from a Zr-metal-organic framework (MOR−1) immobilized on cotton textiles was investigated. The results show that the sorption capacity under passive diffusion uptake conditions, is approximately 20 mg/g for As(VI) and 36 mg/g Se(IV), which is 10 to 30 times lower compared to that determined in batch sorption studies, indicating that conventional equilibrium sorption overestimates the efficiency of the sorbents under realistic conditions. The application of the method to a worst-case scenario, involving the severe contamination of freshwaters with arsenate species, is also demonstrated.

Blue Waters, Green Fields

Controlling future phosphorus (P) loss will not be simple. The main nutrient “culprit” in freshwater contamination, P, becomes available only in its dissolved inorganic form in solution; and that solubility is also what can contaminate the environment if the P ends up in the wrong place. This article examines future P loss measures for agricultural systems. Beyond continued vigilance with regards to best management practices (BMPs), future mitigation tools include physical filters of P loss from crop fields, updating P soil‐test calibrations, stream buffers, cover crops, biologic products that may alter P availability, enzyme additives, and potential P recycling and trading markets. Earn 1.5 CEUs in Soil & Water Management by reading this article and taking the quiz at https://web.sciencesocieties.org/Learning‐Center/Courses.

Application of Cation Resin Regeneration for Ferrous (Fe2+) and Manganese (Mn2+) Removal from Shallow Groundwater using Packed-Bed Column with Thomas Model

One of the factors influencing freshwater contamination is caused by pollutants and organic matter from anthropogenic activities. The appearance of ferrous and manganese metal ions in water systems, especially in shallow groundwater, has become a significant problem. When reacted in the air, an extreme amount of metal ferrous may cause turbidness and severe odor, particularly carrying a dark-brown color to fabrics. Water distribution can also easily be obstructed by iron bacteria in the pipes. It is similar to iron, and manganese has a detrimental effect on the natural environment, human hygiene, and sanitation. The higher influent ferrous and manganese concentration led to the adsorption process getting saturated quicker and a steeper breakthrough curve. The saturation time (ttotal) of the adsorption for ferrous concentration decreased from 430 minute (2 mg/l) to 420 minute (4 mg/l) to 380 minute (6 mg/l) with the elevation of Fe2+ influent concentration. In comparison, the saturation time for manganese concentration dropped from 463 minute (2 mg/l) to 460 minute (4 mg/l) to 420 minute (6 mg/l). Ferrous adsorption capacity (qeq) grew between 0.0046 mg/g and 0.0838 mg/g, followed by a growth in influent concentration. It is similar to manganese. The adsorption performace raised between 0.0216 mg/g and 0.109 mg/g. The Thomas model’s R Square (R2) indicates a better fitting (0.788 – 0.8922) with field data.

Human health risk assessment of metals and metalloids in mining areas of the Northeast Andean foothills of the Ecuadorian Amazon.

Gold mining (GM) is a major source of metals and metalloids in rivers, causing severe environmental pollution and increasing the exposure risks to the residents of surrounding areas. Mining in Ecuadorian Amazonia has dramatically increased in recent years, but its impacts on Indigenous local populations that make use of rivers are still unknown. The aim of this study was to assess the risks to adults and children caused by the exposure to metals and metalloids in freshwater ecosystems contaminated with tailings released by GM activities in 11 sites of the upper Napo River basin, Ecuador. We selected a carcinogenic and a noncarcinogenic risk assessment method to estimate the hazard index (HI) and total cancer risk (TCR). The concentration of Ag, Al, As, Cd, Cu, Fe, Mn, Pb, Zn, B, and V in water and sediment samples was considered to assess the risks to human health. The calculated HI was 23-352 times greater than the acceptable limits in all sites for both children and adults. Mn and Fe were the main contributors (75% in water and 99% in sediment) to the total calculated risk based on the HI. The calculated TCR for children and adults exceeded approximately one to three times the permissible threshold in all sites. As and Pb contributed up to 93% of the total calculated risk based on TCR for both children and adults. This study demonstrates that the emission and mobilization of metals and metalloids caused by mining activities increase the risk to human health, to which we recommend further monitoring of freshwater contamination in the area and the implementation of preventive health management measures. Integr Environ Assess Manag 2023;19:706-716. © 2022 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).

Histopathology unveiling the structural damage in gonads of Catla catla due to freshwater contamination

Potentially harmful materials, e.g., heavy metals, pesticides, dyes, and hydrocarbons, are often released untreated into freshwater bodies. When such pollutants are released in large quantities, there may be large-scale, sudden mortalities of aquatic organisms. This study summarizes experimental evidence about the disruption of gonadal development due to freshwater contamination. Adult fish specimens of Catla catla were collected from the River Chenab downstream of the Chakbandi Main Drain (CMD) entrance into the Chenab River. CMD collects domestic and industrial sewage wastes from Faisalabad city and disposes them into River Chenab, polluting this water body. Water samples were analyzed from each site of fish harvest for water quality parameters. Collected fish specimens were subjected to histopathological evaluation to detect the impact of pollution on the gonads of this fish species. The male Catla catla gonads showed marked histopathological alterations such as testes degeneration, generalized tissue degeneration produced by fragmentation and detachment of basement membrane, necrosis, and fibrosis in the testis. A loss of tubular arrangement, rupturing of the blood vessels, delimiting seminiferous lobules, vascular hypertrophy of the interstitial compartment, and appearance of a clustered organization were observed. The follicular epithelium in vitellogenic oocytes, rupturing and detachment of follicular basement membrane tissue with cytoplasmic vacuolization, immature oocytes with scattered yolk droplets, hypertrophied follicular epithelium, interstitial proteinaceous fluid deposition, and structural deterioration of follicles with early oocytes were observed in the ovary.

Impact of exposure to arsenic on the bacterial microbiota associated with river biofilms in the Pampas region

Freshwater contamination by arsenic (As) is a worldwide problem. It may be found in Pampean streams of Argentina at concentrations higher than those recommended by international organizations and stipulated by national regulations. Exposure to high As concentrations causes serious consequences to both human health and the environment. The general objective of this work was to evaluate the effect of As on the biofilm microbiota structure from Naveira stream, Luján, Province of Buenos Aires (Coordinates: 34º34′02″ S 59º03′51″ W). The biofilm collected was cultivated in glass aquaria at different As III concentrations (0, 0.2 and 20 mg / L), inside incubation chambers under controlled conditions (16 h light: 8 h dark and 24 ± 1 °C) and constant aeration for 31 d, with partial water renewal every 9 d. We amplified the hypervariable regions V3 and V4 of the bacterial 16S rRNA gene from biofilm bacterial community samples to determine the diversity and abundance of the different taxa. The taxonomic composition of each sample, the alpha diversity of each treatment and the main metabolic pathways were analyzed. Principal Component Analysis of the present phyla and a Linear Discriminant Analysis of the metabolic pathways was also performed. Significant changes were observed in relation to the taxonomic composition of the bacterial community after exposure to the metalloid. However, this effect was not observed at the low concentration used (0.2 mg / L), which is the one that corresponds to ecologically relevant levels. The significantly affected phyla were Verrucomicrobiota, Acidobacteriota, Patescibacteria, Hydrogenedentes and WPS-2. The relative abundances of the Verrucomicrobiota, WPS-2 and Patescibacteria groups were notably decreased in the treatment with high As, while the Acidobacteria group was increased in both treatments with As. The stream samples showed greater bacterial diversity than those grown in the laboratory without As. Finally, it was possible to characterize the metabolic profile of the biofilm developed under natural conditions in the leaves of the aquatic plant Elodea canadensis in the Naveira stream. In addition, results showed that biosynthesis-related pathways were more abundant at the high As concentration treatment (20 mg / L).

Identification of Microcrustaceans as Potential Bioindicators of Arsenic in Tropical Water Bodies.

We investigated microcrustaceans inhabiting arsenic contaminated and non-contaminated freshwater to identify potential bioindicators of arsenic contamination in the tropical freshwater of Matehuala in northern Mexico. We collected water, sediment, and zooplankton, at five sampling points during three sampling campaigns. We determined water temperature, pH, electrical conductivity, dissolved oxygen, alkalinity, salinity, and total arsenic concentration in water. Additionally, we determined total arsenic and arsenic speciation in sediment samples. We identified microcrustaceans and determined abundance, richness, and Shannon Index. We also investigated relationships and correlations between physiochemical and ecological variables. Results showed that arsenic concentrations in freshwater ranged from 0.001 to 53.23mg/L, while total arsenic in sediments ranged from 10.37 to 2472.84mg/kg as As + 5. Six microcrustacean species were found in highly and moderately contaminated water (Latonopsis australis, Eucyclops chihuahuensis, Acanthocyclops americanus, Pleuroxus (Picripleuroxus) quasidenticulatus, Macrocyclops albidus, and Paracyclops chiltoni), while five species were found in arsenic-free water (Simocephalus punctatus, Alona glabra, Eucyclops leptacanthus, M. albidus, and P. quasidenticulatus). An inverse relationship was observed between microcrustacean richness and arsenic. However, the scope of the data did not allow for a strong and significant correlation. Nevertheless, among the species inhabiting As-free water, S. punctatus showed potential to be further tested as a bioindicator of As contamination in Matehuala. Identification of potential bioindicators could help monitor water quality and increase understanding of the incorporation and toxicity of As in freshwater-sensitive and freshwater-metallotolerant microcrustaceans, which, in turn, might help us to understand As incorporation in the food web.

Ecological risk of imidacloprid on the Brazilian non-target freshwater organisms Chironomus sancticaroli and Poecilia reticulata.

Imidacloprid (IMI) is a neonicotinoid insecticide widely used in agriculture worldwide. This pesticide has been found in freshwater ecosystems, including Brazilian freshwaters. For this reason, studies are being conducted to detect the presence of IMI in freshwater and understand its effects on the aquatic biota. In the present study, the acute toxic effect of the imidacloprid commercial formulation (ICF) Galeão® on the Brazilian non-target aquatic organisms Chironomus sancticaroli and Poecilia reticulata was evaluated. Enzymatic activities (glutathione S-transferase (GST), catalase (CAT), and ascorbate peroxidase (APX)) were also determined. Moreover, we considered 11 studies that detected IMI concentrations up to 3.65µg.L-1 in 28 different Brazilian freshwaters to evaluate the acute ecological risk of IMI in these environments. From the ecotoxicological assays, we determined the LC50 values for C. sancticaroli (LC50-48h 1.52µg.L-1) and P. reticulata (LC50-96h 122.65mg.L-1). The high sensitivity of C. sancticaroli demonstrates that this species could be used as a bioindicator in studies investigating the contamination of freshwater by IMI. Enzymatic activity changes were observed in both organisms and offered sublethal responses to the effects of the pollution by IMI on aquatic biota. Our results suggest that the presence of IMI in Brazilian aquatic ecosystems can represent a potential ecological risk for the aquatic insect populations and, consequently, cause an imbalance in these ecosystems. The present study provides relevant and comparable toxicity information that may be useful to develop public policies to protect the Brazilian aquatic ecosystem from IMI contamination.

Direct photolysis of contaminants in surface freshwaters, within the equivalent monochromatic wavelength (EMW) approximation

Abiotic photochemical reactions are usually very important degradation pathways for biorecalcitrant pollutants in surface freshwaters. Therefore, the assessment of photolytic lifetimes of contaminants helps estimate their impact on aquatic systems. This is commonly carried out by combining irradiation experiments and modelling, where the latter considers mathematical functions with polychromatic parameters, such as sunlight spectra, photolysis quantum yields (when Kasha's rule does not hold), and absorption coefficients. With the polychromatic approach, the photolytic lifetime is calculated by solving several integrals, which requires quite demanding modelling resources. In this work, we applied a recently developed approach, which is based on the equivalent monochromatic wavelength (EMW) approximation, to compute the direct-photolysis lifetimes of a range of >40 pollutants in inland waters. The EMW approximation allowed for easier modelling procedure, at the same time providing very good agreement with the polychromatic system. To further show EMW potentialities, lifetimes of three contaminants were mapped over the Piedmont region (NW Italy), as an example of how easy it becomes to geographically EMW-assess the potential of watercourses, to get photochemically decontaminated from pollutants.