Freshwater Benthic Macroinvertebrates Research Articles

Effects of Leaf Species and Conditioning State of Fresh Leaves on Colonization by Stream and Pond Macroinvertebrates

Fresh, green leaves are increasing as resources in aquatic ecosystems due to more frequent severe spring and summer storms, but research on allochthonous resources typically uses senescent leaves. We examined macroinvertebrate colonization of green leaves of three native deciduous trees (red maple, red oak, and tulip poplar) over two weeks within both a stream and fishless pond. Leaf colonization varied depending on the taxa of leaves and colonizers, submersion time, and the ecosystem examined. Within the stream, the densities of isopods (Lirceus sp.) and snails (mostly the invasive Potamopyrgus antipodarum) did not vary significantly across leaf species. In contrast, mayflies (Tricorythodes sp.) in the stream colonized tulip poplar in greater numbers than red oak leaves, while higher densities of planarians (order Tricladida) occurred within red oak leaves. The numbers of mayflies and snails decreased significantly by the second week, but the densities of isopods and planaria within stream leaf packs were consistent. In contrast, within the pond, significantly more isopods (Caecidotea communis) were collected after the first than after the second week of submersion and in tulip poplar leaves. Clams (Sphaeriidae) in the pond, on the other hand, were more prevalent in the second week but did not discriminate among leaf species. While the number of leeches (mostly Helobdella stagnalis) was consistent across weeks, significantly fewer leeches resided within tulip poplar leaves than within red oak leaves. Our results suggest that there are no consistent colonization-preference rankings of species of fresh leaves across freshwater benthic macroinvertebrate taxa. Even within a functional feeding group (e.g., the two types of isopods and snails, all detritivore-herbivores), there were differences in colonization patterns. Therefore, increased allochthonous inputs of fresh leaf litter due to severe spring- and summer-time storms are likely to promote the populations of various taxa to different extents.

Towards harmonized standards for freshwater biodiversity monitoring and biological assessment using benthic macroinvertebrates

Monitoring programs at sub-national and national scales lack coordination, harmonization, and systematic review and analysis at continental and global scales, and thus fail to adequately assess and evaluate drivers of biodiversity and ecosystem degradation and loss at large spatial scales. Here we review the state of the art, gaps and challenges in the freshwater assessment programs for both the biological condition (bioassessment) and biodiversity monitoring of freshwater ecosystems using the benthic macroinvertebrate community. To assess the existence of nationally- and regionally- (sub-nationally-) accepted freshwater benthic macroinvertebrate protocols that are put in practice/used in each country, we conducted a survey from November 2022 to May 2023. Responses from 110 respondents based in 67 countries were received. Although the responses varied in their consistency, the responses clearly demonstrated a lack of biodiversity monitoring being done at both national and sub-national levels for lakes, rivers and artificial waterbodies. Programs for bioassessment were more widespread, and in some cases even harmonized among several countries. We identified 20 gaps and challenges, which we classed into five major categories, these being (a) field sampling, (b) sample processing and identification, (c) metrics and indices, (d) assessment, and (e) other gaps and challenges. Above all, we identify the lack of harmonization as one of the most important gaps, hindering efficient collaboration and communication. We identify the IUCN SSC Global Freshwater Macroinvertebrate Sampling Protocols Task Force (GLOSAM) as a means to address the lack of globally-harmonized biodiversity monitoring and biological assessment protocols.

Impacts of divalent cations (Mg2+ and Ca2+) on PFAS bioaccumulation in freshwater macroinvertebrates representing different foraging modes

Per- and polyfluoroalkyl substances (PFAS) have extensively contaminated freshwater aquatic ecosystems where they can be transported in water and partition to sediment and biota. In this paper, three freshwater benthic macroinvertebrates with different foraging modes were exposed to environmentally relevant concentrations of eight perfluoroalkyl carboxylates (PFCA), three perfluoroalkyl sulfonates (PFSA), and three fluorotelomer sulfonates (FTS) at varying divalent cation concentrations of magnesium (Mg2+) and calcium (Ca2+). Divalent cations can impact PFAS partitioning to solids, especially to sediments, at higher concentrations. Sediment dwelling worms (Lumbriculus variegatus), epibenthic grazing snails (Physella acuta), and sediment-dwelling filter-feeding bivalves (Elliptio complanata) were selected due to their unique foraging modes. Microcosms were composed of synthetic sediment, culture water, macroinvertebrates, and PFAS and consisted of a 28-day exposure period. L. variegatus had significantly higher PFAS bioaccumulation than P. acuta and E. complanata, likely due to higher levels of interactions with and ingestion of the contaminated sediment. “High Mg2+” (7.5 mM Mg2+) and “High Ca2+” (7.5 mM Ca2+) conditions generally had statistically higher bioaccumulation factors (BAF) than the “Reference Condition” (0.2 mM Ca2+ and 0.2 mM Mg2+) for PFAS with perfluorinated chain lengths greater than eight carbons. Long-chain PFAS dominated the PFAS profiles of the macroinvertebrates for all groups of compounds studied (PFCA, PFSA, and FTS). These results indicate that the study organism has the greatest impact on bioaccumulation, although divalent cation concentration had observable impacts between organisms depending on the environmental conditions. Elevated cation concentrations in the microcosms led to significantly greater bioaccumulation in the test organisms compared to the experimental reference conditions for long-chain PFAS.

Bioaccumulation of per- and polyfluoroalkyl substances by freshwater benthic macroinvertebrates: Impact of species and sediment organic carbon content

Per- and polyfluoroalkyl substances (PFAS) in aquatic environments have caused global concern due to their persistence, toxicity, and potential bioaccumulation of some compounds. As an important compartment of the aquatic ecosystem, sediment properties impact PFAS partitioning between aqueous and solid phases, but little is known about the influence of sediment organic carbon content on PFAS bioaccumulation in benthic organisms. In this study, three freshwater benthic macroinvertebrates – worms (Lumbriculus variegatus), mussels (Elliptio complanata) and snails (Physella acuta) – were exposed for 28 days to PFAS spiked synthetic sediment equilibrated with a synthetic surface water. Using microcosms, sediment organic carbon content – 2%, 5% and 8% – was manipulated to assess its impact on PFAS bioaccumulation. Worms were found to have substantially greater PFAS bioaccumulation compared to mussels and snails. The bioaccumulation factors (BAFs) and biota sediment accumulation factors (BSAFs) in worms were both one to two magnitudes higher than in mussels and snails, likely due to different habitat-specific uptake pathways and elimination capacities among species. In these experiments, increasing sediment organic carbon content decreased the bioaccumulation of PFAS to benthic macroinvertebrates. In worms, sediment organic carbon content was hypothesized to impact PFAS bioaccumulation by affecting PFAS partitioning and sediment ingestion rate. Notably, the BSAF values of 8:2 fluorotelomer sulfonic acid (FTS) were the largest among 14 PFAS for all species, suggesting that the benthic macroinvertebrates probably have different metabolic mechanisms for fluorotelomer sulfonic acids compared to fish evaluated in published literature. Understanding the impact of species and sediment organic carbon on PFAS bioaccumulation is key to developing environmental quality guidelines and evaluating potential ecological risks to higher trophic level species.

Exposure of Midge Larvae (Chironomus riparius) to Graphene Oxide Leads to Development Alterations.

Despite the fast-growing use and production of graphene-based nanomaterials (GBMs), data concerning their effects on freshwater benthic macroinvertebrates are scarce. This study aims to investigate the effects of graphene oxide (GO) on the midge Chironomus riparius. Mortality, growth inhibition, development delay and teratogenicity, assessed using mentum deformity analysis, were investigated after a 7-day static exposure of the first instar larvae under controlled conditions. The collected data indicated that the survival rate was not impacted by GO, whereas chronic toxicity following a dose-dependent response occurred. Larval growth was affected, leading to a significant reduction in larval length (from 4.4 to 10.1%) in individuals reaching the fourth instar at any of the tested concentrations (from 0.1 to 100 mg/L). However, exposure to GO is not associated with an increased occurrence of mouthpart deformities or seriousness in larvae. These results highlight the suitability of monitoring the larval development of C. riparius as a sensitive marker of GO toxicity. The potential ecological consequences of larval size decrease need to be considered for a complete characterization of the GO-related environmental risk.

Behaviors and biochemical responses of macroinvertebrate Corbicula fluminea to polystyrene microplastics

Microplastic, a well-documented emerging contaminant, is widespread in aquatic environments resulting from the production and fragmentation of large plastics items. The knowledge about the chronic toxic effects and behavioral toxicity of microplastics, particularly on freshwater benthic macroinvertebrates, is limited. In this study, adult Asian clams (Corbicula fluminea) were exposed to gradient microplastic solutions for 42 days to evaluate behavioral toxicity and chronic biotoxicity. The results showed that microplastics caused behavior toxicity, oxidative stress, and tissue damage in high-concentration treatments. Siphoning, breathing, and excretion was significantly inhibited (p < 0.05) at high-concentration treatments, suggesting that high-concentration microplastics induced behavioral toxicity in C. fluminea. Malondialdehyde content, superoxide dismutase, catalase, and glutathione reductase activities were significantly enhanced (p < 0.05) and the acetylcholinesterase was significantly inhibited (p < 0.05) throughout the exposure period in high-concentration treatments. Enzymes associated with energy supply were significantly higher at high-concentration microplastics treatments on D7 and D21. However, they recovered to a normal level on D42. The instability of the enzymes indicated that high-concentration microplastics induced oxidative stress and disorder in neurotransmission and energy supply. The gills of C. fluminea in treatments underwent cilia degeneration, which indicated that microplastics caused tissue damage in the gills. The analysis of integrated biomarker response values revealed that high-concentration microplastics led to long-term effects on the health of C. fluminea. In conclusion, continuous exposure to microplastics (10 mg L−1) would damage physical behavior and the antioxidant system of C. fluminea.

Thiophilicity is a determinant of bioaccumulation in benthic fauna

Aquatic contamination can settle into sediments, where it complexes with organic matter and becomes bioavailable. The resulting bioaccumulation of these contaminants by benthic fauna poses a serious threat due to the potential for trophic transfer. This paper offers an insight into the heterogenous accumulation behavior of different elements, and the consequences for ecological risk. In this study, we present field quantification of sediment-associated bioaccumulation factors (BAFS) in freshwater benthic macroinvertebrates. 17 elements were quantified using ICP-MS in sediment and Asellus aquaticus and Gammarus sp. samples. Previously published reports of contaminant concentrations in freshwater and marine sediments and benthic fauna were likewise analyzed to provide a complementary picture of bioaccumulation across contaminants and taxa. We demonstrate that the BAFS correlates strongly with the thiophilicity of the elemental contaminants, as defined by (Kepp, 2016), for all strata examined. These findings support the hypothesis that thiol-mediated processes, such as that of metallothionein, play a larger role in bioaccumulation than typically afforded. In conclusion, we demonstrate the potential for the thiophilic scale to act as a predictor of accumulation potential.

Elevated levels of per- and polyfluoroalkyl substances (PFAS) in freshwater benthic macroinvertebrates from the Hudson River Watershed

Per- and polyfluoroalkyl substances (PFAS) are contaminants of global concern due to their persistence and associated negative health effects. Considerable attention has been given to monitoring PFAS in the aquatic environment, however, few investigations have done so using freshwater benthic macroinvertebrates (BMIs). As these bottom-dwelling animals are known to bioconcentrate exogenous pollutants to a high degree, studying their PFAS levels may provide a more integrated view of PFAS contamination in the aquatic environment. In this study, BMIs, sediment, and surface water were collected from two streams in the Hudson River Watershed (one historically-impacted by PFAS) and analyzed for 44 PFAS using liquid chromatography–tandem mass spectrometry (LC–MS/MS). Orbitrap high-resolution mass spectrometry (HRMS) was used to confirm the identities of quantitated analytes. Across all matrices, 17 analytes were detected with PFOA dominating in surface water and PFOS in sediment/BMIs. PFOS bioaccumulation factors (BAFs) were approximately one order of magnitude higher than those of PFOA and ranged from 857 to 5151 L kg−1 across different BMI taxa. While PFAS concentrations in surface water and sediment were not excessively high, elevated levels were still measured in most BMI taxa. This observation suggests that the extent of PFAS contamination in a local system may be severely underestimated if only surface water and sediment are used for monitoring. Moreover, these findings have relevance for human exposure assessment considering BMIs are the primary food source of many fish.

Microplastics in freshwater sediments: Effects on benthic invertebrate communities and ecosystem functioning assessed in artificial streams

The high levels of microplastics (MPs) found in freshwaters, particularly in riverine sediments, may impose a threat to the macroinvertebrate communities with possible consequences at ecosystem-level. The present study aimed to assess the effects of a mixture of different sizes of polyethylene microplastics (PE-MPs) on the composition and structure of macroinvertebrate communities and key-functions, such as primary production and leaf litter decomposition. MPs were mixed in the sediment at three different concentrations (0.1, 1, and 10 g kg-1) already found in freshwater sediments to enhance the relevance of the work. After eight days of exposure to PE-MPs, the observed changes in macroinvertebrate community structure were mostly due to the reduction in the abundance of deposit-feeders and grazers that were reduced by ca 31-50% and 34-39%, in the two highest MPs concentrations respectively, in comparison with the control treatment after 8 days of exposure. MPs internal concentrations were detected only in organisms exposed to plastic particles within artificial streams with chironomids and mayflies presenting higher MPs internal levels (average of 115 particles/individual found in chironomids, 166/individual for Baetis sp. and 415 particles/individual for Ephemerella sp.) suggesting higher ingestion of plastic microparticles. Nevertheless, the alterations in the community structure did not translate into impairments in the functional endpoints analysed, leaf litter decomposition and primary production, that were expected due to possible sub-lethal effects (e.g., feeding inhibition) on detritivores and grazers.This study represents one of the few assessments of MPs effects on freshwater benthic macroinvertebrate community structure and the first that simultaneously considered ecosystem-level functional endpoints. Further research combining different microplastics and longer exposure periods are needed to raise knowledge on potential ecological consequences of MPs to freshwaters.

Preliminary indoor evidences of microplastic effects on freshwater benthic macroinvertebrates

Plastics are to date considered one of the main detrimental drivers for the health of aquatic ecosystems, both in marine and inland waters. Regarding the latter habitat, it seems surprising how the plastic effects on benthic invertebrates are neglected since macroinvertebrates have a long tradition in the water quality assessment activities. In this context, we propose timely indoor observations on the exposure of caddisfly Odontocerum albicorne and mayfly Ephemera danica to various microplastic polymers (ABS, PET, PP, PS, PVDF). Three different experimental designs were performed on caddisflies and mayflies by exposing their larvae to natural and microplastic substrates. Our findings highlighted how microplastics affected both caddisflies in rebuilding its own case (after having removed the natural one) and mayflies burrowing. Particularly, all caddisflies rebuilt cases using the microplastic polymers provided instead of natural items only. Moreover, we provide the first evidence that mayflies burrow mainly in microplastic substrates rather than in natural ones. Our research highlights that macroinvertebrate larvae would use naturally occurring microplastics and this could be of particular concern in freshwaters with high contamination by plastics. Indeed, larvae appear to not necessarily perceive microplastics as a direct stressor. Further studies ought to be conducted to understand the chronic perturbation on larvae fitness and for example, on drift behaviour. Also, further investigations are needed to understand the potentialities of using plastics by benthic macroinvertebrates.

Relationship of taxonomic error to frequency of observation.

Biological nomenclature is the entry point to a wealth of information related to or associated with living entities. When applied accurately and consistently, communication between and among researchers and investigators is enhanced, leading to advancements in understanding and progress in research programs. Based on freshwater benthic macroinvertebrate taxonomic identifications, inter-laboratory comparisons of >900 samples taken from rivers, streams, and lakes across the U.S., including the Great Lakes, provided data on taxon-specific error rates. Using the error rates in combination with frequency of observation (FREQ; as a surrogate for rarity), six uncertainty/frequency classes (UFC) are proposed for approximately 1,000 taxa. The UFC, error rates, FREQ each are potentially useful for additional analyses related to interpreting biological assessment results and/or stressor response relationships, as weighting factors for various aspects of ecological condition or biodiversity analyses and helping set direction for taxonomic research and refining identification tools.

Toxicity of polystyrene nanoplastics in dragonfly larvae: An insight on how these pollutants can affect bentonic macroinvertebrates

Although nanoplastics (NPs) are known to be toxic to several groups of animals, the effects of such a toxicity on freshwater benthic macroinvertebrate communities remain unknown. Thus, the aim of the current study is to test the hypothesis that polystyrene nanoplastics (PS NPs) (34 μg/L - 48 h of exposure) lead to biochemical damage in Aphylla williamsoni larvae. Data have evidenced high bioaccumulation factor in the analyzed individuals; this finding indicates that, similar to sediments, water is also part of aquatic systems and favors PS NPs retention in dragonfly larvae. Despite the lack of evidence about the interference of these pollutants in the nutritional status of the analyzed animals, their bioaccumulation was associated with REDOX imbalance featured by concomitant increase in the number of evaluated oxidative stress biomarkers (nitric oxide and lipid peroxidation) and antioxidants (antioxidant activity against the DPPH radical and the superoxide dismutase enzyme). On the other hand, the reduced acetylcholinesterase activity observed in larvae exposed to PS NPs has suggested the neurotoxic effect of these pollutants, with potential impact on their nerve and neuromuscular functions. Therefore, the current study is pioneer in showing that PS NPs can affect the health of the investigated larvae, even at small concentrations, for short exposure-time; this outcome reinforces the ecotoxicological risk of these pollutants for freshwater benthic macroinvertebrates.

How can interspecific interactions in freshwater benthic macroinvertebrates modify trace element availability from sediment?

This study aimed to assess how bioturbation by freshwater benthic macroinvertebrates with different biological traits alone or in combination could modify trace elements (TE) fate between sediment and water, and if water TE concentration and animal TE content impair their body stores. Three macroinvertebrate species were exposed to TE contaminated sediment for 7 days: the omnivorous Echinogammarus berilloni (Amphipoda), the sediment feeding Tubifex tubifex (Oligochaeta) and the filter feeding Pisidium sp. (Bivalvia). Treatments were one without invertebrates (control), two with amphipods or mussels alone, and the combinations amphipod-mussel, and amphipod-mussel-worms. Water TE concentration increased significantly in 2 or 3 species mesocosms, concerning mainly Rare Earth Elements, Cr, U and Pb, known to be associated to the colloidal phase. By contrast, water soluble TE were not affected by animals. For both, amphipods and mussels, TE body content increased with the number of coexisting species. For amphipods, this increase concerned both, soluble and colloid-associated TE, possibly due to intense contact and feeding from sediment and predation on tubificids. TE bioaccumulation in mussel was less important and characterized by soluble TE, with water filtration as most plausible uptake route. Protein, triglyceride and Whole Body Energy Budget increased in amphipods with the number of coexisting species (probably by feeding on mussels' feces and tubificids) whereas triglycerides declined in mussels (presumably filtration was disturbed by amphipods). This study highlights interspecific interactions as key drivers explaining both: TE bioturbation, depending on their water solubility or colloidal association, and the exposure/contamination of species through another species activity.

Insights into the sediment toxicity of personal care products to freshwater oligochaete worms using Fourier transform infrared spectroscopy

Personal care products (PCPs) are ubiquitous in the environment due to their wide use in daily life. However, there are insufficient sediment toxicity data of PCPs under ecologically relevant conditions. Here we used Fourier transform infrared spectroscopy (FTIR) to investigate the sediment toxicity of triclosan (TCS) and galaxolide (HHCB) to two freshwater benthic macroinvertebrates, Limnodrilus hoffmeisteri and Branchiura sowerbyi, in microcosms containing a diverse biological community. Exposure to 8 µg TCS/g and 100 µg HHCB/g dry weight (dw) sediment induced significant biochemical alterations in the L. hoffmeisteri tissue. 8 µg TCS/g primarily affected proteins and nucleic acid while 100 µg HHCB/g mainly affected proteins and lipids of L. hoffmeisteri. However, 0.8 µg TCS/g and 30 µg HHCB/g did not cause significant subcellular toxicity to L. hoffmeisteri. In contrast, exposure of B. sowerbyi to 30 µg HHCB/g led to significant biochemical changes, including proteins, polysaccharides and lipids. Therefore, B. sowerbyi was more sensitive to sediment-associated HHCB than L. hoffmeisteri. Such effects were significantly enhanced when the HHCB concentration increased to 100 µg/g dw where death of B. sowerbyi occurred. These results demonstrate the application of FTIR spectroscopy to sediment toxicity testing of chemicals to benthic invertebrates with biochemical alterations as endpoints that are more sensitive than standard toxic endpoints (e.g., survival and growth).

Comparing Freshwater Benthic Macroinvertebrate Communities in Forest and Urban Streams of the Coastal Ecological Region of Cameroon

In this study conducted in the coastal zone of Cameroon, biological indices and functional feeding groups of benthic macroinvertebrates were used to assess the health status of two urban streams. For a better diagnosis, two streams located in coastal forest zone were used as a reference. Benthic macroinvertebrates were sampled monthly over a 3-month period (from May to July 2017) in six urban stations and six forest stations. Measurements of the physicochemical variables were done simultaneously. Physicochemical analysis revealed that urban streams are strongly polluted with high content of decaying organic matters, while forest streams are slightly polluted as indicated by the Principal Component Analysis. Concerning benthic macroinvertebrates, urban streams are poorly diversified with the proliferation of taxa tolerant to water pollution and belonging to the functional feeding groups of collectors-gatherers. Inversely, forest streams are more diversified and dominated by sensitive taxa, most belonging to the functional feeding groups of predators and shredders. These marked differences between biological indices and feeding mode of benthic macroinvertebrates in forest and urban rivers confirm the reliability of benthic macroinvertebrates as good indicators of freshwater ecosystem in the coastal zone of Cameroon.

How does experimental selective timber harvesting affect invertebrate diversity across different spatial scales in subtropical streams?

In forestry, selective timber harvesting best management practices (BMPs) are widely adopted to mitigate the effects of clearfell harvesting on stream ecosystems. However, there have been surprisingly few studies experimentally assessing the effects of selective harvesting on freshwater benthic macroinvertebrate assemblages and how anthropogenic disturbances impact biodiversity across multiple spatial scales. We assessed how selective timber-harvesting BMPs affected alpha and beta diversity of stream macroinvertebrates when measured across three spatial scales – between harvested impact and unharvested control streams, among pools within streams, and among samples within pools. Using a multiple paired, Before-After Control-Impact design of two pairs of streams draining unharvested control and harvested impact catchments, we sampled stream macroinvertebrates bimonthly over two years before and after selective timber harvesting. Effects of selective harvesting varied between ‘impact’ streams and among different spatial scales. At the whole-stream scale, harvesting did not impact either alpha or beta diversity in both pairs of control-impact streams. Within streams, harvesting temporarily reduced beta diversity in streams with increasingly intermittent flow, yet caused brief increases in beta diversity in increasingly permanently flowing streams. At the finest spatial scale, harvesting had transient variable effects on within-pool beta diversity in one pair of experimental streams but not the other. The absence of impacts of selective harvesting on alpha diversity at all three spatial scales, and the only transient impacts on beta diversity suggest that these BMPs are sufficient to allow timber harvesting while simultaneously preserving headwater stream biodiversity. Our study provides a basis for developing monitoring programs to assess the relative impacts and consequences of anthropogenic disturbances on multi-species assemblages across different spatial scales. Our findings suggest fine scale (within-stream) beta diversity may be a more sensitive indicator of catchment disturbances than comparisons of alpha and beta diversity at broader scales among streams.

Assessing alpha and beta diversities of benthic macroinvertebrates and their environmental drivers between watersheds with different levels of habitat transformation in Japan

Little is known about differences in species diversity among ecological communities subject to different levels of human-caused habitat transformation and how this disturbance contributes to diversity through symbiotic dependencies with the environment in freshwater ecosystems. We estimated α and β diversities of benthic macroinvertebrates and relationships between diversity and environmental variables in Ado River (natural) and Yasu River (intermediately disturbed) watersheds, Japan. Alpha diversity was consistently slightly higher in the natural river watershed than in the intermediately disturbed one, but the spatial distribution was not equivalent. The opposite pattern was found for β diversity. Significant differences in environmental variables existed between the two river watersheds, with especially high chlorophyll-a concentrations detected in the intermediately disturbed watershed. Alpha diversity was not correlated with specific environmental variables, whereas water temperature and chlorophyll-a concentrations were the two most significant environmental variables influencing β diversity across sites in the two watersheds. These results suggest that diversity patterns in freshwater benthic macroinvertebrates are differentially influenced by levels of human-caused habitat transformation, especially that intermediately disturbed habitats may benefit species turnover, and further understanding how they relate to environmental variables is essential for protecting local to regional diversity and can provide useful information for conservation planning to maximise biodiversity at the watershed scale.

Ingestion and Chronic Effects of Car Tire Tread Particles on Freshwater Benthic Macroinvertebrates.

Micronized particles released from car tires have been found to contribute substantially to microplastic pollution, triggering the need to evaluate their effects on biota. In the present study, four freshwater benthic macroinvertebrates were exposed for 28 days to tread particles (TP; 10–586 μm) made from used car tires at concentrations of 0, 0.1, 0.3, 1, 3, and 10% sediment dry weight. No adverse effects were found on the survival, growth, and feeding rate of Gammarus pulex and Asellus aquaticus, the survival and growth of Tubifex spp., and the number of worms and growth of Lumbriculus variegatus. A method to quantify TP numbers inside biota was developed and here applied to G. pulex. In bodies and faces of G. pulex exposed to 10% car tire TP, averages of 2.5 and 4 tread particles per organism were found, respectively. Chemical analysis showed that, although car tire TP had a high intrinsic zinc content, only small fractions of the heavy metals present were bioavailable. PAHs in the TP-sediment mixtures also remained below existing toxicity thresholds. This combination of results suggests that real in situ effects of TP and TP-associated contaminants when dispersed in sediments are probably lower than those reported after forced leaching of contaminants from car tire particles.

Microplastic Effect Thresholds for Freshwater Benthic Macroinvertebrates.

Now that microplastics have been detected in lakes, rivers, and estuaries all over the globe, evaluating their effects on biota has become an urgent research priority. This is the first study that aims at determining the effect thresholds for a battery of six freshwater benthic macroinvertebrates with different species traits, using a wide range of microplastic concentrations. Standardized 28 days single species bioassays were performed under environmentally relevant exposure conditions using polystyrene microplastics (20–500 μm) mixed with sediment at concentrations ranging from 0 to 40% sediment dry weight (dw). Microplastics caused no effects on the survival of Gammarus pulex, Hyalella azteca, Asellus aquaticus, Sphaerium corneum, and Tubifex spp. and no effects were found on the reproduction of Lumbriculus variegatus. No significant differences in growth were found for H. azteca, A. aquaticus, S. corneum, L. variegatus, and Tubifex spp. However, G. pulex showed a significant reduction in growth (EC10 = 1.07% sediment dw) and microplastic uptake was proportional with microplastic concentrations in sediment. These results indicate that although the risks of environmentally realistic concentrations of microplastics may be low, they still may affect the biodiversity and the functioning of aquatic communities which after all also depend on the sensitive species.

Utilisation rationnelle de fertilisants organiques pour la production de macroinvertébrés benthiques d’eau douce en pisciculture

Description du sujet. Cette étude détermine les conditions optimales d’utilisation de déjections de lapin dans la production de macroinvertébrés benthiques pour une production de poisson à faible cout. Objectifs. L’objectif de cette étude est la détermination de la dose optimale de déjections pour une production plurispécifique de macroinvertébrés d’eau douce. Méthode. Pour ce faire, six traitements dont un témoin ont reçu respectivement 0 %, 10 %, 25 %, 50 %, 75 % et 100 % de déjections par rapport au volume total de substrat. Les cultures ont été réalisées dans des seaux qui ont chacun reçu au total 10 dm3 de substrat, 16 dm3 d’eau de forage et 4 dm3 d’eau d’étang. La densité initiale d’ensemencement est de 6 ind·dm-2 d’Odonates et d’Annélides (soit 3 ind·dm-3, pour une profondeur de 0,5 m), 8 ind·dm-2 de Chironomidae (soit 4ind·dm-3) et 10 ind·dm-2 de Mollusques (soit 5 ind·dm-3). Les paramètres physico-chimiques et biologiques de la culture ont été suivis tout au long des 63 jours d’expérimentation. Résultats. Les densités les plus élevées de macroinvertébrés benthiques sont obtenues avec le traitement T2 (192 ± 1,20 ind·dm-2 de Chironomidae, 52 ± 0,8 ind·dm-2 de Mollusques, 6 ± 0,33 ind·dm-2 d’Odonates et 4 ± 0,33 ind·dm-2 d’Annélides soit, 96 ± 1,20 ind·dm-3 de Chironomidae, 26 ± 0,8 ind·dm-3 de Mollusques, 3 ± 0,33 ind·dm-3 d’Odonates et 2 ± 0,33 ind·dm-3 d’Annélides). La biomasse la plus élevée des Chironomidae et des Mollusques est obtenue avec T2, soit respectivement 728,1 ± 2,23 mg matière sèche·dm-2 et 699,98 ± 22,49 mg matière sèche·dm-2 (ou encore respectivement 364,05 ± 2,23 mg matière sèche·dm-3 et 349,99 ± 22,49 mg matière sèche·dm-3). Conclusions. Au regard de ces résultats, le traitement T2 (140 g de matière sèche·dm-2, soit 75 g de déjections de lapin sèches·dm-3 de substrat) constituerait la dose à recommander pour une production plurispécifique optimale de macroinvertébrés benthiques d’eau douce.